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Fish are generally classified as herbivorous, carnivorous, omnivorous, or filter-feeders according to feeding habits, including the feeding method and food content. The feeding habits of fish are reflected in their digestive organ, mainly the intestine. The intestine tract is the main site for digestion and nutritional uptake, and it is regarded as a key organ in fish nutrition. Reportedly, fish gut microbiota contributes to digestion and affect gastrointestinal tract development as well as the overall growth of the fish. The disruption of the intestinal microbiota induced by their feeding habit via diet usually affects the digestive functions of the host through disturbance in bacterial digestive enzyme production. Feeding habits determine the feed composition, which shapes the microbial communities in fish. For example, diet has been reported as a dominant cause of variation in the microbiota composition of rainbow trout. In contrast, feeding habit, which is regulated by many factors, including intrinsic and external factors, such as appetite, structure of the digestive tract, and feed palatability, is an important area of research in the intestinal microflora study. The feeding habit of fishes is one of the main factors affecting the differential colonization of fish intestinal flora. For example, the feeding habit increases the colonization of fish intestinal flora in the order omnivorous > herbivorous > carnivorous. It is also known that feeding habits influence intestinal digestion and structure. However, the influence of feeding habit on proximate composition, digestive enzyme secretion, and intestinal histomorphology is currently not well-known in fish. On the contrary, the feeding habit affects the composition and diversity of the intestinal microflora of fish. The composition and diversity of the microbiota in Carassius auratus, Ctenopharyngodon idella, Siniperca chuatsi, and Schizothorax grahami from the same environment have also not previously been studied. The present study aimed to compare and analyze the differences in the composition and diversity of the intestinal microflora of four fish species, investigating the relationship between their feeding habits and intestinal microflora as well. This study collected 12 intestine samples from each of the four fishes with different feeding habits, including omnivorous (C. auratus), herbivorous (C. idella), filter-feeder (S. chuatsi), and carnivorous (S. grahami), from the same pond. The composition and diversity of the microbial communities were determined by using high-throughput sequencing of 16S rRNA. Meanwhile, this study investigated the effects of feeding habits on the composition and diversity of the intestinal microflora of fish and predicted the potential functions of specific microflora with different nutrients. The results showed that the diversity of the intestinal microflora in fish was significantly affected by feeding habit (P < 0.05), such that higher levels occurred in the order omnivorous (C. auratus) > herbivorous (C. idella) > filter-feeder (S. grahami) > carnivorous (S. chuatsi). Proteobacteria and Firmicutes were the common dominant flora among the four fishes, although there were differences at the genera and species levels. For example, Acinetobacter and Bacteroides species were the most dominant flora in S. chuatsi and C. idella, respectively. Function prediction showed that the intestinal tract of S. chuatsi was mainly gram-negative. The disease resistance potential of C. idella was slightly higher than that of the other three fish species. Acinetobacter johnsonii, Acinetobacter lwoffii, and Pseudomonas stutzeri might help the host to digest protein, while Bacteroidetes may help the host to digest cellulose. In conclusion, feeding habit is one of the main factors affecting the differential colonization of fish intestinal flora. Analyzing the correlation between feeding habits and dominant intestinal flora as well as exploring the function of specific flora, could lay a theoretical foundation for studying the microbial effect of fish nutrition metabolism.

Immunotherapy has emerged as the primary treatment modality for patients with advanced Hepatocellular carcinoma (HCC). However, its clinical efficacy remains limited, benefiting only a subset of patients, while most exhibit immune tolerance and face a grim prognosis. The infiltration of immune cells plays a pivotal role in tumor initiation and progression. In this study, we conducted an analysis of immune cell infiltration patterns in HCC patients and observed a substantial proportion of CD8+T cells. Leveraging the weighted gene co-expression network analysis (WGCNA), we identified 235 genes associated with CD8+T cell and constructed a risk prediction model. In this model, HCC patients were stratified into a high-risk and low-risk group. Patients in the high-risk group exhibited a lower survival rate, predominantly presented with intermediate to advanced stages of cancer, displayed compromised immune function, showed limited responsiveness to immunotherapy, and demonstrated elevated expression levels of the Notch signaling pathway. Further examination of clinical samples demonstrated an upregulation of the Notch1+CD8+T cell exhaustion phenotype accompanied by impaired cytotoxicity and cytokine secretion functions that worsened with increasing Notch activation levels. Our study not only presents a prognostic model but also highlights the crucial involvement of the Notch pathway in CD8+T cell exhaustion—a potential target for future immunotherapeutic interventions.

The study aimed to investigate the potential of traditional Chinese medicine (TCM) in reducing the risk of macrovascular invasion (MVI) in Chinese patients with hepatocellular carcinoma (HCC). This retrospective analysis involved 2,267 HCC patients treated at our hospital. Propensity score (PS) matching was used to compare TCM users (n = 485) with non-users (n = 485) in terms of age, Barcelona Clinic Liver Cancer (BCLC) staging, type of treatment, and AFP. The impact of TCM on the hazard ratio (HR) of MVI was evaluated using a Cox multivariate regression model. The efficacy of TCM therapy on MVI was further examined using the log-rank test. The analysis revealed that TCM medication was a significant protective factor for MVI in HCC patients, as evidenced by the Cox analysis (adjusted HR = 0.496, 95% CI: 0.387–0.635, p < 0.001). After PS matching, the Kaplan-Meier curve demonstrated a lower occurrence rate of MVI in TCM users compared to non-users. The study findings suggest that TCM treatment has the potential to decrease the incidence of MVI in HCC patients, irrespective of etiology, BCLC staging, liver function, or treatment type. Notably, as the use of TCM increased, the percentage of MVI in patients showed a gradual decrease, indicating the potential of TCM therapy as a successful strategy for preventing MVI.

Abstract We explore the potential of the adjoint‐state tsunami inversion method for rapid and accurate near‐field tsunami source characterization using S‐net, an array of ocean bottom pressure gauges. Compared to earthquake‐based methods, this method can obtain more accurate predictions for the initial water elevation of the tsunami source, including potential secondary sources, leading to accurate water height and wave run‐up predictions. Unlike finite‐fault tsunami source inversions, the adjoint method achieves high‐resolution results without requiring densely gridded Green's functions, reducing computation time. However, optimal results require a dense instrument network with sufficient azimuthal coverage. S‐net meets these requirements and reduces data collection time, facilitating the inversion and timely issuance of tsunami warnings. Since the method has not yet been applied to dense, near‐field data, we test it on synthetic waveforms of the 2011 Mw 9.0 Tohoku earthquake and tsunami, including triggered secondary sources. The results indicate that with a static source model without noise, using the first 5 min of the waveforms yields a favorable performance with an average accuracy score of 93%, and the largest error of predicted wave amplitudes ranges between −5.6 and 1.9 m. Using the first 20 min, secondary sources were clearly resolved. We also demonstrate the method's applicability using S‐net recordings of the 2016 Mw 6.9 Fukushima earthquake. The findings suggest that lower‐magnitude events require a longer waveform duration for accurate adjoint inversion. Moreover, the estimated stress drop obtained from inverting our obtained tsunami source, assuming uniform slip, aligns with estimations from recent studies.

ABSTRACTThe control of Ostrinia furnacalis, a major pest of maize in Xinjiang, is challenging owing to the occurrence of resistant individuals. Entomopathogenic fungi (EPF) are natural insect regulators used as substitutes for synthetic chemical insecticides. The fungus Aspergillus nomius is highly pathogenic to O. furnacalis; however, its virulence characteristics have not been identified. This study aimed to analyse the lethal efficacy, mode of infection on the cuticle, and extracellular enzyme activity of A. nomius against O. furnacalis. We found that the mortality and mycosis of O. furnacalis were dose-dependent when exposed to A. nomius and varied at different life stages. The egg-hatching and adult emergence rates decreased with an increase in conidial suspension. The highest mortality (83.33%, 7 d post-infection [DPI]) and mycosis (74.33%, 7 DPI) and the lowest mortality response (8.52 × 103 conidia mL−1) and median lethal time (4.91 d) occurred in the 3rd instar larvae of O. furnacalis. Scanning electron microscopy indicated that numerous conidia germination and infection structure formation may have contributed to the high pathogenicity of A. nomius against O. furnacalis. There were significant correlations between O. furnacalis mortality and the activities of extracellular protease, lipase, and chitinase of A. nomius. This study revealed the infection process of the highly pathogenic A. nomius against O. furnacalis, providing a theoretical basis and reference for strain improvement and field application of EPF.

Multiple lines of evidence indicate that the 2023 Mw 7.8 Kahramanmaraş (Türkiye) earthquake started on a splay fault, then branched bilaterally onto the nearby East Anatolian Fault (EAF). This rupture pattern includes one feature previously deemed implausible, called backward rupture branching: rupture propagating from the splay fault onto the SW EAF segment through a sharp corner (with an acute angle between the two faults). To understand this feature, we perform 2.5-D dynamic rupture simulations considering a large set of possible scenarios. We find that both subshear and supershear ruptures on the splay fault can trigger bilateral ruptures on the EAF, which themselves can be either subshear, supershear, or a mixture of the two. In most cases, rupture on the SW segment of the EAF starts after rupture onset on its NE segment: the SW rupture is triggered by the NE rupture. Only when the EAF has initial stresses very close to failure can its SW segment be directly triggered by the initial splay-fault rupture, earlier than the activation of the NE segment. These results advance our understanding of the mechanisms of multi-segment rupture and the complexity of rupture processes, paving the way for a more accurate assessment of earthquake hazards.

Abstract Fish feeding habit determines the digestive tract structure and intestinal microflora. However, the relationship between feeding habit, digestive intestinal morphology, and microbial diversity of omnivorous, herbivorous, plankton feeder, and carnivorous fish from the same environment has not been compared. This study compared the digestive enzyme activities, intestinal morphology, and intestinal microflora of omnivorous (Carassius auratus), herbivorous (Ctenopharyngodon idellus), carnivorous (Siniperca chuatsi), and plankton feeder (Schizothorax grahami) fishes and predicted the potential functions of specific microflora on different nutrients. Twelve intestine samples were collected from each of the four fishes from Dianchi Lake. The composition and diversity of microbial communities were determined by using high‐throughput sequencing of 16S rDNA. The results showed that the carnivorous fish (S. chuatsi) had higher trypsin and pancrelipase activities in the hepatopancreas and enteropeptidase in the intestine, but lower amylase activities in the intestine. The carnivorous fish intestine had more microvilli branches and complex structures than other fish species in the order carnivorous > herbivorous > plankton feeder > omnivorous. The intestinal microflora diversity was higher in the omnivorous fish and followed the order omnivorous > herbivorous > plankton feeder > carnivorous. Acinetobacter species and Bacteroides species were the most dominant flora in the carnivorous and herbivorous fishes, respectively. Acinetobacter species and Pseudomonas species might help the host to digest protein, while Bacteroidetes species may help the host to digest cellulose. Taken together, feeding habit determines the digestive enzyme activities, intestinal tissue morphology, and differential colonization of fish intestinal flora. The knowledge obtained is useful in feed formulation and feeding practices for the studied fish species.

Abstract Objective To identify the key drugs of Yangyin Fuzheng Jiedu prescription (YFJP) and investigate their therapeutic effects against hepatocellular carcinoma (HCC) and the potential mechanism using network pharmacology. Methods The H22 tumor‐bearing mouse model was established. Thirty male BALB/c mice were divided randomly into five groups. The mice were orally treated with either disassembled prescriptions of YFJP or saline solution continuously for 14 days. The mice were weighed every 2 days during treatment and the appearance of tumors was observed by photographing. The tumor inhibition rate and the spleen and thymus indexes were calculated. Hematoxylin and eosin and immunohistochemical staining were performed to observe the histological changes and tumor‐infiltrating lymphocytes. Cell apoptosis was determined by terminal deoxynucleotidyl transferase‐mediated dUTP nick‐end labeling staining. The proportion of CD8+ T cells and the expression of programmed cell death protein 1 (PD‐1), T cell immunoglobulin domain and mucin domain‐3 (Tim‐3), and T cell immunoreceptor with Ig and ITIM domains (TIGIT) were analyzed using flow cytometry. The production of serum cytokines was detected using the Milliplex® MAP mouse high sensitivity T cell panel kit. The active components of the key drugs and HCC‐related target proteins were obtained from the corresponding databases. The putative targets for HCC treatment were screened by target mapping, and potential active components were screened by constructing a component‐target network. The interactive targets of putative targets were obtained from the STRING database to construct the protein–protein interaction network. Gene ontology (GO) and Kyoto encyclopedia of genes and genomes pathway enrichment analyses were performed based on potential targets. The gene–gene inner and component‐target‐pathway networks were constructed and analyzed to screen the key targets. Western blotting was used to evaluate the protein expression of the key targets in the tumor‐bearing mouse model. The binding activity of the key targets and compounds was verified by molecular docking. Results Among the three disassembled prescriptions of YFJP, the Fuzheng prescription (FZP) showed significant antitumor effects and inhibited weight loss during the treatment of H22 tumor‐bearing mice. FZP increased the immune organ index and the levels of CD8+ and CD3+ T cells in the spleen and peripheral blood of H22 tumor‐bearing mice. FZP also reduced the expression of PD‐1, TIGIT, and TIM3 in CD8+ T cells and the production of IL‐10, IL‐4, IL‐6, and IL‐1β. Network pharmacology and experimental validation showed that the key targets of FZP in the treatment of HCC were PIK3CA, TP53, MAPK1, MAPK3, and EGFR. The therapeutic effect on HCC was evaluated based on HCC‐related signaling pathways, including the PIK3‐Akt signaling pathway, PD‐L1 expression, and PD‐1 checkpoint pathway in cancer. GO enrichment analysis indicated that FZP positively regulated the molecular functions of transferases and kinases on the cell surface through membrane raft, membrane microarea, and other cell components to inhibit cell death and programmed cell death. Conclusion FZP was found to be the key disassembled prescription of YFJP that exerted antitumor and immunoregulatory effects against HCC. FZP alleviated T cell exhaustion and improved the immunosuppressive microenvironment via HCC‐related targets, pathways, and biological processes.

Conjunctival melanoma (CM) is a rare and fatal malignant eye tumor. In this study, we deciphered a novel anti-CM mechanism of a natural tetracyclic compound named as cucurbitacin B (CuB). We found that CuB remarkably inhibited the proliferation of CM cells including CM-AS16, CRMM1, CRMM2 and CM2005.1, without toxicity to normal cells. CuB can also induce CM cells G2/M cell cycle arrest. RNA-seq screening identified KIF20A, a key downstream effector of FOXM1 pathway, was abolished by CuB treatment. Further target identification by activity-based protein profiling chemoproteomic approach revealed that GRP78 is a potential target of CuB. Several lines of evidence demonstrated that CuB interacted with GRP78 and bound with a Kd value of 0.11 μmol/L. Furthermore, ATPase activity evaluation showed that CuB suppressed GRP78 both in human recombinant GRP78 protein and cellular lysates. Knockdown of the GRP78 gene significantly induced the downregulation of FOXM1 and related pathway proteins including KIF20A, underlying an interesting therapeutic perspective. Finally, CuB significantly inhibited tumor progression in NCG mice without causing obvious side effects in vivo. Taken together, our current work proved that GRP78–FOXM1–KIF20A as a promising pathway for CM therapy, and the traditional medicine CuB as a candidate drug to hinder this pathway.

ABSTRACT: Bovine tuberculosis (bTB) caused by Mycobacterium bovis is an important zoonotic disease. This infection is difficult to control because of the limited ability of the tuberculin skin test (TST) and ancillary IFN-γ release assay to detect all infected animals. In this study, we aimed to develop an efficient assay based on the enzyme-linked immunospot (ELISpot) technique for the diagnosis of bTB, with IFN-γ monoclonal antibodies 3E9 and Bio-labeled 6F8 used as capture and detection antibodies, respectively. As expected, there were significantly more M. bovis-specific spot-forming units (SFU) in bTB-infected cattle than in healthy cattle when an M. bovis-specific antigen, CFP-10-ESAT-6 fusion protein (CE protein), was used. The M. bovis IFN-γ ELISpot assay demonstrated a high level of agreement (90.83%) with the BOVIGAM ELISA test (Thermo Fisher Scientific) for detecting bTB. Furthermore, 3 of 109 cattle tested negative by both the TST and the BOVIGAM ELISA tests, but positive by the ELISpot assay (TST− ELISA− ELISpot+). During subsequent long-term monitoring, these 3 cattle became TST+ ELISA+ ELISpot+. These results suggest that the M. bovis IFN-γ ELISpot assay we established could detect infected cattle earlier than the BOVIGAM ELISA test.

ABSTRACT Bacterial defense barriers, such as DNA methylation-associated restriction-modification (R-M) and the CRISPR-Cas system, play an important role in bacterial antimicrobial resistance (AMR). Recently, a novel R-M system based on DNA phosphorothioate (PT) modification has been shown to be widespread in the kingdom of Bacteria as well as Archaea. However, the potential role of the PT R-M system in bacterial AMR remains unclear. In this study, we explored the role of PT R-Ms in AMR with a series of common clinical pathogenic bacteria. By analyzing the distribution of AMR genes related to mobile genetic elements (MGEs), it was shown that the presence of PT R-M effectively reduced the distribution of horizontal gene transfer (HGT)-derived AMR genes in the genome, even in the bacteria that did not tend to acquire AMR genes by HGT. In addition, unique gene variation analysis based on pangenome analysis and MGE prediction revealed that the presence of PT R-M could suppress HGT frequency. Thus, this is the first report showing that the PT R-M system has the potential to repress HGT-derived AMR gene acquisition by reducing the HGT frequency. IMPORTANCE In this study, we demonstrated the effect of DNA PT modification-based R-M systems on horizontal gene transfer of AMR genes in pathogenic bacteria. We show that there is no apparent association between the genetic background of the strains harboring PT R-Ms and the number of AMR genes or the kinds of gene families. The strains equipped with PT R-M harbor fewer plasmid-derived, prophage-derived, or integrating mobile genetic element (iMGE)-related AMR genes and have a lower HGT frequency, but the degree of inhibition varies among different bacteria. In addition, compared with Salmonella enterica and Escherichia coli, Klebsiella pneumoniae prefers to acquire MGE-derived AMR genes, and there is no coevolution between PT R-M clusters and bacterial core genes.

By performing a conformal optical mapping of an input beam from Cartesian to log-polar coordinates, we propose a method to generate vortex beam. By tilting the incident beam, a phase gradient is attached and evolves into a vortex phase due to the optical transformation. Thus, the topological charge of the generated vortex beam is continuously adjustable. With this method, vortex beams with integer and fractional orders are generated. The purity of the generated vortex beam is theoretically analyzed, as well as the possible effects of phase misalignment on the output vortex. The continuously tunable vortex beam achieved by this method is expected to be used in information processing and optical routing in optical communications.

Hepatocellular carcinoma (HCC) is a severe cancer endangering human health. We constructed a novel glycometabolism-related risk score to predict prognosis and immunotherapy strategies in HCC patients. The HCC data sets were obtained from the Cancer Genome Atlas (TCGA) and the Gene Expression Omnibus (GEO) database, and the glycometabolism-related gene sets were obtained from the Molecular Signature Database. The least absolute contraction and selection operator (LASSO) regression model was used to construct a risk score based on glycometabolism-related genes. A simple visual nomogram model with clinical indicators was constructed and its effectiveness in calibration, accuracy, and clinical value was evaluated. We also explored the correlation between glycometabolism-related risk scores and molecular pathways, immune cells, and functions. Patients in the low-risk group responded better to anti-CTLA-4 immune checkpoint treatment and benefited from immune checkpoint inhibitor (ICI) therapy. The study found that glycometabolism-related risk score can effectively distinguish the prognosis, molecular and immune-related characteristics of HCC patients, and may provide a new strategy for individualized treatment.

Introduction: Among all immune cells, natural killer (NK) cells play an important role as the first line of defense against tumor. The purpose of our study is to observe whether the NK cell counts can predict the overall survival of patients with hepatocellular carcinoma (HCC). Methods: To develop a novel model, from January 2010 to June 2015, HCC patients enrolled in Beijing Ditan hospital were divided into training and validation cohort. Cox multiple regression analysis was used to analyze the independent risk factors for 1-year, 3-year and 5-year overall survival (OS) of patients with HCC, and the nomogram was used to establish the prediction model. In addition, the decision tree was established to verify the contribution of NK cell counts to the survival of patients with HCC. Results: The model used in predicting overall survival of HCC included six variables (namely, NK cell counts, albumin (ALB) level, alpha-fetoprotein (AFP) level, portal vein tumor thrombus (PVTT), tumor number and treatment). The C-index of nomogram model in HCC patients predicting 1-year, 3-year and 5-year overall survival was 0.858, 0.788 and 0.782 respectively, which was higher than tumor–lymph node–metastasis (TNM) staging system, Okuda, model for end-stage liver disease (MELD), MELD-Na, the Chinese University Prognostic Index (CUPI) and Japan Integrated Staging (JIS) scores (p < 0.001). The decision tree showed the specific 5-year OS probability of HCC patients under different risk factors, and found that NK cell counts were the third in the column contribution. Conclusions: Our study emphasizes the utility of NK cell counts for exploring interactions between long-term survival of HCC patients and predictor variables.

Abstract Background Yangyin Fuzheng Jiedu Prescription (YFJP) is a traditional Chinese medicine (TCM) indicated for the treatment of hepatocellular carcinoma (HCC). Its potential targets and molecular mechanisms are not clear. Therefore, this study intends to explore the molecular mechanism of YFJP based on network pharmacology analysis and in vitro validation. Methods and results Through univariate and multivariate analyses and survival analysis in HCC patients with or without YFJP treatment we found that drinking alcohol, alfafeto protein ≥ 400 ng/l, baseline portal vein tumor thrombus and total bilirubin level ≥ 18.8 μM) were independent risk factors for poor prognosis, while red blood cell count ≥ 4 × 109/l and TCM treatment were independent protective factors. Besides, YFJP prolonged the cumulative survival of HCC patients. Using online pharmacological methods, we obtained 58 relevant compounds and molecular 53 targets. By using scratch test, Transwell assay, EdU assay, and TUNEL staining, we found that YFJP-containing serum repressed the migration, invasion and proliferation of HCC cells in vitro, and induced cell apoptosis. Moreover, YFJP diminished the gene expression of TP53, CCND1, p-EGFR, EGF, VEGFA, JUN, IL6, COX-2, AKT1, and MAPK1 in HCC cells, but elevated the expression of ESR1 and CASP3. Conclusions Taken together, results showed that YFJP attenuated HCC progression through mediating effects on HCC-related genes.

The wide usage of 3D mesh models greatly increases the importance of an effective matching algorithm for them. In this paper, we propose a novel 3D model matching algorithm. Firstly, vertices on the input 3D mesh models are mapped to 1D space by employing Isomap. A pose-invariant feature set is then constructed from the vertices in 1D space. Finally, the similarity between any two 3D models can be computed by comparing their feature sets. Experimental results show that the algorithm is not only invariant to translation, rotation, scaling, but also invariant to different poses of 3D models. Additionally, the algorithm is robust to noise.

We experimentally realize fractal-like photonic lattices by use of the cw-laser-writing technique, thereby observing distinct compact localized states (CLSs) associated with different flatbands in the same lattice setting. Such triangle-shaped lattices, akin to the first generation Sierpinski lattices, possess a band structure where singular non-degenerate and nonsingular degenerate flatbands coexist. By proper phase modulation of an input excitation beam, we demonstrate not only the simplest CLSs but also their superimposition into other complex mode structures. Our experimental and numerical results are corroborated by theoretical analysis. Furthermore, we show by numerical simulation a dynamical oscillation of the flatband states due to beating of the CLSs that have different eigenenergies. These results may provide inspiration for exploring fundamental phenomena arising from the interplay of fractal structure, flatband singularity, and real-space topology.

In this paper, the waste polyethylene terephthalate (PET) was glycolysed by trimethylolpropane with zinc acetate as catalyst. The effects of different content glycolysis product of waste PET on the appearance, viscosity, particle size and molecular weight of autoxidative water-reducible alkyd resins and the corresponding film adhesion, flexibility, impact resistance, gloss, hardness and chemical resistance were studied. Meanwhile, experimental results were compared with commercial water-reducible alkyd and water-reducible alkyd without the glycolysis product of waste PET. The results show that the maximum concentration of PET in autoxidative water-reducible alkyd resins can reach 8.5 wt%, and the molecular weight, particle size and viscosity of water-reducible alkyd resin do not change much with the increase of PET concentration. The introduction of PET resulted in the viscosity of water-reducible alkyd resins being greater than that of water-reducible alkyd resin without PET; this is mainly because PET contains harder terephthalic acid monomer units. However, the particle size of water-reducible alkyd resins with waste PET is significantly lower than that of the water-reducible alkyd resin without PET; this is due to PET-free water-reducible alkyd resin containing more pentaerythritol with greater steric hindrance. In addition, the hardness of the water-reducible alkyd resin paint film (PET content is 8.5%) reaches 1H, which is higher than the hardness (HB) of the water-reducible alkyd resin paint film without PET and the commercial alkyd resin paint film, while the physical properties and chemical resistance of the former are comparable to those of the latter two kinds of paint films. Therefore, the use of waste PET in water-borne coatings systems not only reduces the cost of coatings, but also opens up a new market for recycled PET, which may contribute a promising method for management of waste PET.

Immunotherapy has emerged as the primary treatment modality for patients with advanced Hepatocellular carcinoma (HCC). However, its clinical efficacy remains limited, benefiting only a subset of patients, while most exhibit immune tolerance and face a grim prognosis. The infiltration of immune cells plays a pivotal role in tumor initiation and progression. In this study, we conducted an analysis of immune cell infiltration patterns in HCC patients and observed a substantial proportion of CD8+T cells. Leveraging the weighted gene co-expression network analysis (WGCNA), we identified 235 genes associated with CD8+T cell and constructed a risk prediction model. In this model, HCC patients were stratified into a high-risk and low-risk group. Patients in the high-risk group exhibited a lower survival rate, predominantly presented with intermediate to advanced stages of cancer, displayed compromised immune function, showed limited responsiveness to immunotherapy, and demonstrated elevated expression levels of the Notch signaling pathway. Further examination of clinical samples demonstrated an upregulation of the Notch1+CD8+T cell exhaustion phenotype accompanied by impaired cytotoxicity and cytokine secretion functions that worsened with increasing Notch activation levels. Our study not only presents a prognostic model but also highlights the crucial involvement of the Notch pathway in CD8+T cell exhaustion--a potential target for future immunotherapeutic interventions. [ABSTRACT FROM AUTHOR]

We recently found that SspABCD, catalyzing single-stranded (ss) DNA phosphorothioate (PT) modification, coupled with SspE provides protection against phage infection. SspE performs both PT-simulated NTPase and DNA-nicking nuclease activities to damage phage DNA, rendering SspA-E a PT-sensing defense system.

Abstract Background Control of Mycobacterium tuberculosis (Mtb) infection requires CD4+ T-cell responses and major histocompatibility complex class II (MHC II) presentation of Mtb antigens (Ags). Dendritic cells (DCs) are the most potent of the Ag-presenting cells and are central to the initiation of T-cell immune responses. Much research has indicated that DCs play an important role in anti-mycobacterial immune responses at early infection time points, but the kinetics of Ag presentation by these cells during these events are incompletely understood. Results In the present study, we evaluated in vivo dynamics of early Ag presentation by murine lymph-node (LN) DCs in response to Mycobacterium bovis bacillus Calmette–Guérin (BCG) Ag85A protein. Results showed that the early Ag-presenting activity of murine DCs induced by M. bovis BCG Ag85A protein in vivo was transient, appearing at 4 h and being barely detectable at 72 h. The transcription levels of CIITA, MHC II and the expression of MHC II molecule on the cell surface increased following BCG infection. Moreover, BCG was found to survive within the inguinal LN DC pool, representing a continuing source of mycobacterial Ag85A protein, with which LN DCs formed Ag85A peptide-MHCII complexes in vivo. Conclusions Our results demonstrate that a decrease in Ag85A peptide production as a result of the inhibition of Ag processing to is largely responsible for the short duration of Ag presentation by LN DCs during BCG infection in vivo.