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Objective. To explore the effects and satisfaction of comfort nursing plus psychological nursing in the clinical nursing of neurology patients. Methods. In this prospective study, 90 neurology patients admitted to our hospital from January 2019 to January 2020 were recruited and randomized into a control group and an experimental group with 45 cases in each group. The control group received routine care, and the experimental group received comfort care plus psychological care. The comfort scores and nursing satisfaction of the two groups were compared. The Hospital Anxiety and Depression Scale (HAD) was used to assess the emotional state of patients before and after the intervention. The Exercise of Self-care Agency Scale (ESCA) was used to assess the patient’s self-care ability after the intervention. Results. The comfort scores of the experimental group were higher than those of the control group ( P < 0.05 ). The experimental group showed significantly higher satisfaction than the control group ( P < 0.05 ). Patients in the experimental group had lower HAD scores after intervention than those in the control group ( P < 0.05 ). After the intervention, the experimental group had higher ESCA scores and a higher Barthel index than the control group ( P < 0.05 ). Conclusion. Comfort nursing plus psychological care improves nursing satisfaction and self-care ability of neurology patients and relieves their negative emotions.

PurposeIt is an important style classification way to sort out suits according to the button arrangement. However, since the different dressing ways of suit cause the buttons to be easily occluded, the traditional identification methods are difficult to identify the details of suits, and the recognition accuracy is not ideal. The purpose of this paper is to solve the problem of fine-grained classification of suit by button arrangement. Taking men's suits as an example, a method of coordinate position discrimination algorithm combined faster region-based convolutional neural network (R-CNN) algorithm is proposed to achieve accurate batch classification of suit styles under different dressing modes.Design/methodology/approachThe detection algorithm of suit buttons proposed in this paper includes faster R-CNN algorithm and coordinate position discrimination algorithm. Firstly, a small sample base was established, which includes six suit styles in different dressing states. Secondly, buttons and buttonholes in the image were marked, and the image features were extracted by the residual network to identify the object. The anchors regression coordinates in the sample were obtained through convolution, pooling and other operations. Finally, the position coordinate relation of buttons and buttonholes was used to accurately judge and distinguish suit styles under different dressing ways, so as to eliminate the wrong results of direct classification by the network and achieve accurate classification.FindingsThe experimental results show that this method could be used to accurately classify suits based on small samples. The recognition accuracy rate reaches 95.42%. It can effectively solve the problem of machine misjudgment of suit style due to the cover of buttons, which provides an effective method for the fine-grained classification of suit style.Originality/valueA method combining coordinate position discrimination algorithm with convolutional neural network was proposed for the first time to realize the fine-grained classification of suit style. It solves the problem of machine misreading, which is easily caused by buttons occluded in different suits.

During rapid proliferation and metabolism, tumor cells show a high dependence on methionine. The deficiency of methionine exhibits significant inhibition on tumor growth, which provides a potential therapeutic target in tumor therapy. Herein, ClO2-loaded nanoparticles (fluvastatin sodium&metformin&bupivacaine&ClO2@CaSiO3@MnO2-arginine-glycine-aspatic acid (RGD) (MFBC@CMR) NPs) were prepared for synergistic chlorine treatment and methionine-depletion starvation therapy. After outer layer MnO2 was degraded in the high glutathione (GSH) tumor microenvironment (TME), MFBC@CMR NPs released metformin (Me) to target the mitochondria, thus interfering with the tricarboxylic acid (TCA) cycle and promoting the production of lactate. In addition, released fluvastatin sodium (Flu) by the NPs acted on monocarboxylic acid transporter 4 (MCT4) in the cell membrane to inhibit lactate leakage and induce a decrease of intracellular pH, further prompting the NPs to release chlorine dioxide (ClO2), which then oxidized methionine, inhibited tumor growth, and produced large numbers of Cl- in the cytoplasm. Cl- could enter mitochondria through the voltage-dependent anion channel (VDAC) channel, which was opened by bupivacaine (Bup). The disruption of Cl- homeostasis promotes mitochondrial damage and membrane potential decline, leading to the release of cytochrome C (Cyt-C) and apoptosis inducing factor (AIF) and further inducing cell apoptosis. To sum up, the pH-regulating and ClO2-loaded MFBC@CMR nanoplatform can achieve cascade chlorine treatment and methionine-depletion starvation therapy toward tumor cells, which is of great significance for improving the clinical tumor treatment effect.

Hydrogen peroxide (H2O2) is a remarkably strong oxidant, and its vapour ([H2O2]g) has further advantages, such as a low cost and good light transmission. However, there has been very little research on its removal through gas-phase advanced oxidation (GPAO). In the present study, the photochemical oxidation of a gas that contains a series of benzene derivatives using ultraviolet (UV) irradiation and [H2O2]g was investigated in a transparent bag made of fluorinated ethylene propylene (FEP). UV and [H2O2]g barely reduced the pollutant within 5 h when used alone, and the reactant was also stable. When the pollutant concentration was high (248 to 756 mg/m3) and the residence time was short (3 s) compared with related studies on the removal of benzene, toluene and xylene, the apparent removal rate by UV/[H2O2]g/(powder active carbon, PAC) was higher than when other methods (UV/[H2O2]g, UV/[H2O2]g/TiO2 and UV/[H2O2]g/ZnO), were used. However, it was found that the mineralization by UV/[H2O2]g significantly decreased, which in turn decreased the conductivity after the reaction. Increasing the pollutant concentration and the pH of the H2O2 had a negative effect on the treatment, but the UV radiation had a positive effect at powers of up to 40 W. In addition, the characteristic absorbance of three benzene derivatives showed that the key structure of the pollutant molecules was damaged during GPAO. Supplementary Information The online version contains supplementary material available at 10.1007/s11356-021-16920-w.

High temperatures are a major threat to plant growth and development, leading to yield losses in crops. Calcium-dependent protein kinases (CPKs) act as critical components of Ca2+ sensing in plants that transduce rapid stress-induced responses to multiple environmental stimuli. However, the role of CPKs in plant thermotolerance and their mechanisms of action remain poorly understood. To address this issue, tomato (Solanum lycopersicum) cpk28 mutants were generated using a CRISPR-Cas9 gene-editing approach. The responses of mutant and wild-type plants to normal (25°C) and high temperatures (45°C) were documented. Thermotolerance was significantly decreased in the cpk28 mutants, which showed increased heat stress-induced accumulation of reactive oxygen species (ROS) and levels of protein oxidation, together with decreased activities of ascorbate peroxidase (APX) and other antioxidant enzymes. The redox status of ascorbate and glutathione were also modified. Using a yeast two-hybrid library screen and protein interaction assays, we provide evidence that CPK28 directly interacts with cytosolic APX2. Mutations in APX2 rendered plants more sensitive to high temperatures, whereas the addition of exogenous reduced ascorbate (AsA) rescued the thermotolerance phenotype of the cpk28 mutants. Moreover, protein phosphorylation analysis demonstrated that CPK28 phosphorylates the APX2 protein at Thr-59 and Thr-164. This process is suggested to be responsive to Ca2+ stimuli and may be required for CPK28-mediated thermotolerance. Taken together, these results demonstrate that CPK28 targets APX2, thus improving thermotolerance. This study suggests that CPK28 is an attractive target for the development of improved crop cultivars that are better adapted to heat stress in a changing climate.

Objectives To identify novel genetic loci associated with systemic lupus erythematosus (SLE) and to evaluate potential genetic differences between ethnic Chinese and European populations in SLE susceptibility. Methods A new genome-wide association study (GWAS) was conducted from Jining, North China, on 1506 individuals (512 SLE cases and 994 matched healthy controls). The association results were meta-analysed with existing data on Chinese populations from Hong Kong, Guangzhou and Central China, as well as GWAS results from four cohorts of European ancestry. A total of 26 774 individuals (9310 SLE cases and 17 464 controls) were included in this study. Results Meta-analysis on four Chinese cohorts identifies KLF2 as a novel locus associated with SLE [rs2362475; odds ratio (OR) = 0.85, P=2.00E-09]. KLF2 is likely an Asian-specific locus as no evidence of association was detected in the four European cohorts (OR = 0.98, P =0.58), with evidence of heterogeneity (P=0.0019) between the two ancestral groups. Meta-analyses of results from both Chinese and Europeans identify STAB2 (rs10082873; OR= 0.89, P=4.08E-08) and DOT1L (rs4807205; OR= 1.12, P=8.17E-09) as trans-ancestral association loci, surpassing the genome-wide significance. Conclusions We identified three loci associated with SLE, with KLF2 a likely Chinese-specific locus, highlighting the importance of studying diverse populations in SLE genetics. We hypothesize that DOT1L and KLF2 are plausible SLE treatment targets, with inhibitors of DOT1L and inducers of KLF2 already available clinically.

Nitric oxide (NO) gas treatment offers a promising strategy for tumor therapy; however, its practical application is still limited due to its poor efficacy and biotoxicity which were caused by gas leakage during blood delivery. Herein, a nano-platform (CMH-OBN) composed of chlorin e6-melanin-hyaluronic acid nanoparticles (Ce6-MNP-HA, CMH) and oxidized bletilla striata polysaccharide microcapsules (Oxi-BSP) carrying NO donors was prepared for responsive and cascaded release of NO, reactive oxygen species (ROS) and its secondary metabolite reactive nitrogen species (RNS) in tumor sites. Melanin not only endowed CMH with good photothermal properties, but also helped Ce6 to produce a large number of ROS under near-infrared (NIR) irradiation. OBN microcapsules, which were sensitive to ROS, can release NO donors under the stimulation of ROS released by CMH nanoparticles under NIR irradiation and can further release NO in the tumor microenvironment (TME) with high expression of glutathione (GSH). NO could further up-regulate soluble guanylate cyclase-cyclic guanosine monophosphate (sGC-cGMP) signal pathways to relieve hypoxia, thus further enhancing the photodynamic therapy (PDT). Moreover, the cascaded release of ROS and NO could produce RNS with higher lethality, which could sequentially initiate the cellular apoptotic procedure and promote immunotherapy by activating T cells at the tumor sites. More interestingly, the CMH-OBN nano-platform could supply magnetic resonance imaging (MRI) and infrared photothermal imaging guidance for tumor therapy. In conclusion, the development of a CMH-OBN nano-platform provides a satisfactory demonstration by combining NO therapy with photothermal therapy (PTT), PDT and immunotherapy for the treatment of cancer.

Changes in leaf length, width, area, weight, chlorophyll and carotenoids contents, and photosynthetic variables with different leaf positions were investigated in fruit cucumber. Plants were grown on rockwool slabs in an environmentally controlled greenhouse and irrigated by drip fertigation. Leaf measurements were conducted from the first to the 15th leaf (the oldest to the youngest). The results showed that fresh weight per unit leaf area decreased from the second to the 15th leaf. Changes in cucumber leaf length, width, and area followed quadratic models from the first to the 15th leaf. The quadratic models of leaf length, width, and area fit the measurements well, with R2 values of 0.925, 0.951, and 0.955, respectively. The leaf chlorophyll a and b and carotenoid contents increased from the oldest leaf (first leaf) to the youngest leaf and decreased after reaching the highest values. Changes in the net photosynthetic rate (Pn) also followed the quadratic model from the first to the 15th leaf, with R2 values of 0.975. The leaf transpiration rate (Tr) increased from the first to the 14th leaf. Our results revealed patterns in leaf growth and photosynthetic changes at different leaf positions in fruit cucumber and improved our understanding of the growth and development of fruit cucumber in the greenhouse production system.

Adequate greenhouse environmental management is very important for improving resource use efficiency and increasing vegetable yield. The objective of this study was to explore suitable climate and cultivation management for cucumber to achieve high yield and build optimal yield models in semi-closed greenhouses. A fruit cucumber cultivar Deltastar was grown over 4 years in greenhouse and weekly data of yields (mean, highest and lowest) and environmental variables, including total radiation, air temperature, relative humidity, and carbon dioxide (CO2) concentration were collected. Regression analyses were applied to develop the relationships and build best regression models of yields with environmental variables using the first 2 years of data. Data collected in years 3 and 4 were used for model validation. Results showed that total radiation, nutrient, temperature, CO2 concentration, and average nighttime relative humidity had significant correlations with cucumber yields. The best regression models fit the mean, lowest, and highest yields very well with R2 values of 0.67, 0.66, and 0.64, respectively. Total radiation and air temperature had the most significant contributions to the variations of the yields. Our results of this study provide useful information for improving greenhouse climate management and yield forecast in semi-closed greenhouses.

Dysfunction of the calcium balancing system and disruption of calcium distribution can induce abnormal intracellular calcium overload, further causing serious damage and even cell death, which provides a potential therapeutic approach for tumor treatment. Herein, a nano-platform, which includes UCNPs-Ce6@RuR@mSiO

Hydrogen peroxide (H2O2) has the remarkable characteristics of strong oxidation, in which its vapour ([H2O2]g)has further advantages, such as economize and good light transmission. But there is very rare research about its degradation effect in Gas-Phase Advanced Oxidation (GPAO). In the present study, the photochemical oxidation for gas containing benzene series, using ultraviolet (UV) irradiation and [H2O2]g, was investigated in a transparent bag made of fluorinated ethylene propylene (FEP). Only UV or [H2O2]g hardly reduced the pollutant in 5 hours in which the reactor was also stable. With the high pollutant concentration (248 to 756 mg/m3) and short residence time (3S) compared with the related studies, studying the degradation of benzene, toluene and xylene, the apparent rate by UV/[H2O2]g/(powder active carbon, PAC) was higher compared with the different way (UV/[H2O2]g, UV/[H2O2]g/TiO2 and UV/[H2O2]g/ZnO), but it was found that the terminal degradation by UV/[H2O2]g significantly decreased, in which the conductivity decreased after reaction. The effects of increasing pollutant concentration and H2O2 pH were negative for the treament, but the UV radiated power positive till 40W. In addition, the characteristic absorbance for three benzene series showed that the key structure of pollutant molecule were damaged in GPAO.

Considering the authoritative engineering education certification standards is of great significance to formulate a curriculum system that meets the actual needs of engineering talents. However, the existing research on intelligent optimization of curriculum system pays little attention to certification standards. In this paper, a mathematical model of course selection and balance problem is established with the optimization goal of the support degree of curriculum system based on Washington Accord Graduate Attribute and the balance of academic load between semesters, and a two-stage improved genetic firefly algorithm(GAFA) is proposed to solve the model. Finally, the feasibility and effectiveness of the proposed model and algorithm are verified by taking the actual training requirements and course data as examples.

Microplastic pollution in marine environments and organisms has received a great deal of international attention. However, the long-term field studies of microplastics are rare. Here, we assessed annual variation in microplastic abundance in the Maowei Sea, a classic mariculture bay in southern China, and analyzed the long-term accumulation in oyster tissues. U-shaped time trends of microplastics in water were observed from January to December in 2018 in the estuarine region, inner bay, and mouth bay sites, representing an inverse relationship with the local rainfall patterns. The common microplastic particles in Maowei Sea are PET/PE fibers, and polystyrene foams, which are mainly related to textile pollution and fishery activities. After one year of continuous monitoring, we did not find accumulation of microplastics in the whole soft tissues of oyster after 10% KOH digestion. No significant correlation of microplastic abundances between water and oysters was observed. The microplastic abundance in oyster was correlated with some environmental variables (i.e. salinity, pH, nutrients and total organic carbon) of the surrounding water following Spearman correlation analysis. The microplastic levels in oysters could probably be influenced by the environmental variables.

Countries throughout the whole world, including China, are working together to curb the greenhouse effect, but the effects seem very limited in spite of the fact that various low-carbon development strategies have been adopted, particularly in industrial enterprises. Therefore, carbon emissions caused by the public should be taken seriously, and the public should be encouraged to engage in behavior that limits carbon emissions. Therefore, this article introduces a new incentive mechanism called the Carbon Generalized System of Preferences (CGSP), which was first introduced in Guangdong Province, China. It is believed that this new mechanism matches the role of leadership in Guangdong in the urbanization and economic development of China by addressing the small sources of greenhouse gases (GHGs) and by issuing carbon coins. Compared with Chinese Certified Emission Reduction (CCER), the development scope, management level, and novel criteria of CGSP are very different but relatively easy for the public to accept. The CGSP shows that the network platform, reduced carbon emissions, and urban pilots are all compatible with the goals of the nation and city, and they promote the CGSP in different ways. Because of its consistency with ecological civilization in China, the prospect of the CGSP is bright; however, there are some challenges, such as policy and economic factors, combined with pollution control.

Dysfunction of the calcium balancing system and disruption of calcium distribution can induce abnormal intracellular calcium overload, further causing serious damage and even cell death, which provides a potential therapeutic approach for tumor treatment. Herein, a nano-platform, which includes UCNPs-Ce6@RuR@mSiO2 @PL-HA NPs (UCRSPH) and SA-CaO2 nanoparticles, is prepared for improving the tumor micro-environment (TME), Ca2+ signal disturbance as well as enhanced photodynamic tumor therapy (PDT). UCRSPH combined with SA-CaO2 can alter TME and relieve hypoxia of the tumor to realize self-reinforcing PDT under near-IR irradiation (980 nm). The ruthenium red (RuR) in the UCRSPH NPs can be released to the cytoplasm after endocytosis of the nanoparticles, target Ca2+ channel proteins on the endoplasmic reticulum and mitochondria, sarcoplasmic reticulum Ca2+ -ATPase (SERCA), and mitochondrial calcium uniporter (MCU). The combined participation of nanoparticles and RuR promotes Ca2+ imbalance and cytoplasmic calcium overload with the assistance of CaO2 , and provides tumor cells higher sensitivity to PDT. Furthermore, the nano-platform also provides fluorescence imaging and calcification computed tomography imaging for in vivo treatment guidance. In conclusion, this image-guided nano-platform show potential for highly specific, efficient combined therapy against tumor cells with minimal side-effects to normal cells by integrating TME improvement, self-reinforcing PDT, and Ca2+ signal disturbance.

To investigate the responses of plant photosynthesis and chlorophyll fluorescence to excessive sulfur fumigation stress (Sulfur) alleviated by pre-treated salt in the soil (Salt), seedlings of a tomato cultivar (Solanum lycopersicum L. ‘Money Maker’) were exposed to the Sulfur with or without the Salt treatment for 15 h. Leaf fresh weight, dry weight, chlorophyll (Chl) content, carotenoid (Car) content, photosynthesis and chlorophyll fluorescence parameters were measured. The results showed that the Sulfur treatment significantly decreased leaf fresh weight, dry weight, Chl and Car contents, net photosynthetic rate (Pn), stomatal conductance (Gs), transpiration rate (Tr), maximum quantum efficiency of photosystem II (PSII) photochemistry (Fv/Fm), actual photochemical efficiency of PSII (ФPSII), photochemical quenching coefficient (qP), electron transport rate (ETR), and the effective quantum yield of PSII photochemistry (F′v/F′m). In addition, the Sulfur treatment significantly increased intercellular CO2 concentration (Ci) and non-photochemical quenching coefficients (qN). Plants in soil pre-treated with salt clearly experienced less damage from sulfur toxicity, and these plants were able to retain higher leaf mass per area, greater total Chl and Car contents and photosynthetic activities, and to maintain the integrity of their chlorophyll fluorescence systems than fumigated plants growing in soil that was not pre-treated. Reduced leaf photosynthesis in the Sulfur treatment was due to non-stomatal limitation, and to disturbance of the chlorophyll fluorescence system which needs more energy consumption for thermal dissipation. Our results showed that a cross-tolerance exists between the salt stress and extreme sulfur fumigation stress, and plants pre-treated with salt provided some protection against excessive sulfur toxicity.

The current study aims at comparatively investigating the effect of apparent density on flame retardancy, thermal degradation and combustion behaviors of rigid polyurethane foam (RPUF), RPUF/ expandable graphite (EG) and RPUF/ ammonium polyphosphate (APP). A series of RPUF, RPUF/EG and RPUF/APP samples with different apparent densities (30, 60 and 90 kg/m3) were prepared. The flame retardancy, thermal degradation, and combustion behaviors of each sample were investigated. Limiting oxygen index (LOI) results indicated that increasing apparent density was beneficial to the flame retardancy of all foam systems. The effect of apparent density on the enhancement of flame retardancy followed the sequence of RPUF &lt, RPUF/APP &lt, RPUF/EG. Thermogravimetric analysis (TGA) results showed that an increase in the apparent density can cause more weight loss in the first degradation stage and less weight loss in the second degradation stage for all foam systems. The combustion behaviors also showed significant differences. The samples with a higher apparent density showed a longer duration of heat release and higher total heat release (THR). The findings in this study demonstrated that apparent density played an important role in flame retardancy, thermal degradation, and combustion behaviors of RPUF, which must be paid more attention in the studies of flame-retardant RPUF.

Hypothesis Organic foamy materials possess good thermal insulation properties and inorganic materials are non-combustible. Hence, it is possible to develop a kind of organic-inorganic lightweight thermal insulation materials with excellent fire safety. Experiments Hollow glass microsphere (HGM), as one kind of lightweight noncombustible inorganic material, was chosen as the filling material. Phenolic resin (PR), as the flame retardant polymeric material, was used as binding material. A series of HGM/PR composites with various PR/HGM mass ratio were prepared. Properties, such as apparent density, microstructure, mechanical strength, thermal conductivity, burning behavior and flame retardancy of the specimens were determined, respectively. Findings The results show that the surface of HGM particles is coated by a layer of cured PR and the HGM powder is glued together firmly from the scanning electron microscope (SEM) images. With the increase of PR/HGM mass ratio, both of apparent density and mechanical strength of HGM/PR composites increase, but thermal conductivity and limiting oxygen index (LOI) values decrease, all of the specimens still possess high LOI value (>50%). What’s more, no flaming combustion (merely partial carbonization) and hardly any smoke can be observed during the burning process, which indicates the HGM/PR composites possess excellent flame retardant property and fire safety.

Mercury contamination is a serious problem in the Hg mining area of Xiushan County, Chongqing, southwestern China. The concentrations of total mercury (THg) and methylmercury (MeHg) in paddy soil, surface water, and rice (grain) samples were determined to investigate the regional distribution of Hg contamination. Simultaneously, gaseous elemental Hg (GEM) in ambient air near the sampling sites was measured. The total Hg concentrations in surface water were highly elevated, ranging from 13 to 2390 ng/L, and the total MeHg concentrations varied between 0.17 and 1.1 ng/L. The dissolved Hg and MeHg concentrations in surface water ranged from 4.7 to 470 ng/L and 0.14–0.35 ng/L, respectively. High THg and MeHg concentrations were also obtained in paddy soils from mining areas, ranging from 0.45 to 68 μg/g and 0.13–4.8 ng/g, respectively. Similar to the high concentrations in water and soil, the THg concentration in rice (grain) ranged from 4.7 to 550 ng/g and MeHg from 2.9 to 26 ng/g. Elevated Hg concentrations in rice, as a staple food of local residents, confirmed that rice consumption could be a vital pathway for MeHg exposure to native people. Humic acid and fulvic acid had significant correlations with soil MeHg, implying that they have important roles that influence MeHg production in soil. The spatial distribution characteristics of Hg and MeHg pollution in the local environment indicated their origins from historic Hg mining sites in the Xiushan area.