Search

Your search keyword '"Yung Lee Suen"' showing total 3 results
Start Over Author "Yung Lee Suen"
3 results on '"Yung Lee Suen"'

1. DHA-rich marine microalga Schizochytrium mangrovei possesses anti-ageing effects on Drosophila melanogaster

Author

Jieqiong Huangfu, Jin Liu, Cheng Peng, Yung Lee Suen, Mingfu Wang, Yue Jiang, Zhen-Yu Chen, and Feng Chen

Subjects
Ageing, Microalgae, Schizochytrium mangrovei, Drosophila melanogaster, Stress-defense, Nutrition. Foods and food supply, TX341-641
Abstract

Docosahexaenoic acid (DHA) plays important roles in the prevention of ageing and ageing-related diseases. In this work, the anti-ageing effect of Schizochytrium mangrovei (SM), a marine microalga with high contents of DHA was investigated for the first time. In vitro results showed that SM enhanced the vitality of healthy pheochromocytoma (PC12) cells, whereas it had no protective effect under H2O2-induced oxidative stress. Meanwhile, SM exerted neuroprotective actions in vivo: the supplementation of SM significantly extended the lifespan of both wild type and SODn108 mutant flies Drosophila melanogaster, and ameliorated the age-related decline of locomotor function. This lifespan-extending activity was associated with the mRNA up-regulation of endogenous stress-defense genes such as CuZn-superoxide dismutase (SOD1) and Mn-superoxide dismutase (SOD2), as well as the down-regulation of Methuselah (MTH) gene. Therefore, it was concluded that the anti-ageing capability of DHA-rich SM was mediated through the cooperation with endogenous stress-defense system rather than the direct interaction with oxidative free radicals.

Published
2013
Full Text
View/download PDF

3. Enhanced production of fatty acids and astaxanthin in Aurantiochytrium sp. by the expression of Vitreoscilla hemoglobin

Author

Yung Lee Suen, Feng Chen, Hongmei Tang, and Junchao Huang

Subjects
chemistry.chemical_classification, Expression vector, Zeocin, Metabolite, Fatty Acids, Wild type, Fatty acid, Heterologous, Truncated Hemoglobins, General Chemistry, Biology, Xanthophylls, chemistry.chemical_compound, chemistry, Biochemistry, Bacterial Proteins, Metabolic Engineering, Astaxanthin, Fermentation, Vitreoscilla, General Agricultural and Biological Sciences, Stramenopiles, Biotechnology
Abstract

Dissolved oxygen is a critical factor for heterotrophic cell growth and metabolite production. The aim of this study was to investigate the effects of an oxygen-involved protein on cell growth and fatty acid and astaxanthin production in the biologically important thraustochytrid Aurantiochytrium sp. The hemoglobin of the Vitreoscilla stercoraria (VHb) gene was fused upstream with a zeocin resistance gene (ble) and driven by the Aurantiochytrium tubulin promoter. The expression construct was introduced into two strains of Aurantiochytrium sp. by electroporation. Transgenic Aurantiochytrium sp. strains MP4 and SK4 expressing the heterologous VHb achieved significantly higher maximum biomass than their corresponding controls in microaerobic conditions. Furthermore, the transformants of Aurantiochytrium sp. SK4 produced 44% higher total fatty acid and 9-fold higher astaxanthin contents than the wild type control in aerobic conditions. The present study highlights the biotechnological application of VHb in high-cell density fermentation for enhanced biomass production as well as high-value metabolites.

Published
2014

Catalog

Books, media, physical & digital resources
See catalog results