35 results on '"Yun-Feng Lu"'
Search Results
2. On-spot preparation of EDTA solution for the treatment of band keratopathy: a case report
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Yan-Ting Li, Bin Chen, Tian-Hong Wu, Bo Jiang, and Yun-Feng Lu
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Ophthalmology ,RE1-994 - Published
- 2024
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3. Engineering Escherichia coli for high-yielding 2,5-Dimethylpyrazine synthesis from L-Threonine by reconstructing metabolic pathways and enhancing cofactors regeneration
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Xin-Xin Liu, Yao Wang, Jian-Hui Zhang, Yun-Feng Lu, Zi-Xing Dong, Chao Yue, Xian-Qing Huang, Si-Pu Zhang, Dan-Dan Li, Lun-Guang Yao, and Cun-Duo Tang
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2,5-dimethylpyrazine ,Metabolic engineering ,Microbial cell factories ,Aminoacetone oxidase ,Threonine transporter ,Whole-cell catalysis ,Biotechnology ,TP248.13-248.65 ,Fuel ,TP315-360 - Abstract
Abstract 2,5-Dimethylpyrazine (2,5-DMP) is important pharmaceutical raw material and food flavoring agent. Recently, engineering microbes to produce 2,5-DMP has become an attractive alternative to chemical synthesis approach. In this study, metabolic engineering strategies were used to optimize the modified Escherichia coli BL21 (DE3) strain for efficient synthesis of 2,5-DMP using L-threonine dehydrogenase (EcTDH) from Escherichia coli BL21, NADH oxidase (EhNOX) from Enterococcus hirae, aminoacetone oxidase (ScAAO) from Streptococcus cristatus and L-threonine transporter protein (EcSstT) from Escherichia coli BL21, respectively. We further optimized the reaction conditions for synthesizing 2,5-DMP. In optimized conditions, the modified strain can convert L-threonine to obtain 2,5-DMP with a yield of 2897.30 mg/L. Therefore, the strategies used in this study contribute to the development of high-level cell factories for 2,5-DMP. Graphical Abstract
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- 2024
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4. Two new phyllospheric species of Colacogloea (Colacogloeaceae, Pucciniomycotina) identified in China
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Yun-Feng Lu, Chun-Yue Chai, and Feng-Li Hui
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Botany ,QK1-989 - Abstract
During our ongoing survey of basidiomycetous yeasts associated with plant leaves in virgin forest, five Colacogloea strains were isolated in the Baotianman Nature Reserve, Henan Province, central China. Phenotypes from cultures and a phylogeny based on the internal transcribed spacer (ITS) regions and the D1/D2 domains of the large subunit (LSU) rRNA gene were employed to characterize and identify these isolates. As a result, two new species, namely Colacogloea celtidis sp. nov. and C. pararetinophila sp. nov., are introduced herein. In the phylogeny of combined ITS and LSU dataset, the new species C. celtidis sp. nov. formed a clade with the unpublished Colacogloea strain (KBP: Y-6832), and together these formed the sister group to C. armeniacae, while C. pararetinophila sp. nov. was retrieved as a sister to C. retinophila. A detailed description and illustration of both new species, as well as the differences between them and their closest relatives in the genus are provided. Results from the present study will add to our knowledge of the biodiversity of Colacogloea in China.
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- 2024
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5. Two new Erythrobasidium species inhabiting the phyllosphere discovered in the Baotianman Nature Reserve in China
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Yun-Feng Lu, Chun-Yue Chai, and Feng-Li Hui
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Basidiomycota ,Erythrobasidiaceae ,phylogenetic analysis ,orange-red yeast ,leaf ,Microbiology ,QR1-502 - Abstract
The genus Erythrobasidium is kind of species-scarce and undersampling basidiomycetes. Currently, only six species have been accepted into the genus and the diversity still remains incompletely understood. In this study, five Erythrobasidium strains were isolated in the surface of plant leaves collected from the Baotianman Nature Reserve, Henan Province, central China. Phylogenetic analyses of the small ribosomal subunit (SSU) rRNA gene, the internal transcribed spacer (ITS) region, the D1/D2 domain of the large subunit (LSU) rRNA gene, and the translation elongation factor 1-α (TEF1-α) gene coupled with morphological studies were employed to characterize and identify these isolates. As a result of these, two new species, namely E. turpiniae sp. nov. and E. nanyangense sp. nov., were delimited and proposed based on morphological and molecular evidence. A detailed description and illustration of both new species, as well as their differences with the close relatives in the genus are provided. An identification key for Erythrobasidium species is also provided. This study provides further insights into our understanding of Erythrobasidium species.
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- 2024
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6. High-level expression of an acetaldehyde dehydrogenase from Lactiplantibacillus plantarum and preliminary evaluation of its potential as a functional food additive
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Zhe Wang, Jian-Ju He, Xin-Xin Liu, Hong-Ling Shi, Yun-Feng Lu, Jun-Ye Shi, Yun-Chao Kan, Lun-Guang Yao, and Cun-Duo Tang
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Acetaldehyde dehydrogenase ,Alcohol tolerance ,Bioinformatic analysis ,Heterologous expression ,High-level expression ,Lactobacillus plantarum ,Biotechnology ,TP248.13-248.65 ,Biology (General) ,QH301-705.5 - Abstract
Background: Acetaldehyde dehydrogenase (ALDH) is a common oxidoreductase and one of the aldehyde dehydrogenases, which can convert toxic acetaldehyde to harmless acetyl CoA. Results: A novel acetaldehyde dehydrogenase (LpALDH) from Lactiplantibacillus plantarum was obtained by gene mining and then was analyzed by a series of bioinformatics software. Phylogenetic tree results showed that LpALDH was highly homologous with acetaldehyde dehydrogenase derived from Pediococcus sp., and their sequence similarity was 62.9%. Moreover, it was successfully expressed in Escherichia coli BL21, its expression level was 195 U/mL, which was about 600 times than that expressed in L. plantarum. After purification by affinity chromatography, the specific activity of reLpALDH was 1709 U/mg. Its temperature optimum was 35°C, and the optimal reaction pH was 8.0. Moreover, the purified LpALDH showed good stability in simulated gastrointestinal fluids, which indicated that it had great potential in functional food additive field. Conclusions: This research also laid a solid foundation for further molecular modification, optimal expression host selection and application of acetaldehyde dehydrogenases.How to cite: Wang Z, He J, Liu X, et al. High-level expression of an acetaldehyde dehydrogenase from Lactiplantibacillus plantarum and preliminary evaluation of its potential as a functional food additive. Electron J Biotechnol 2023;63. https://doi.org/10.1016/j.ejbt.2022.12.005.
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- 2023
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7. Expression of Novel L-Leucine Dehydrogenase and High-Level Production of L-Tert-Leucine Catalyzed by Engineered Escherichia coli
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Yuan-Yuan Jia, Yu-Li Xie, Lu-Lu Yang, Hong-Ling Shi, Yun-Feng Lu, Si-Pu Zhang, Cun-Duo Tang, Lun-Guang Yao, and Yun-Chao Kan
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leucine dehydrogenase ,feed additive ,expression ,biocatalysis ,formate dehydrogenase ,Biotechnology ,TP248.13-248.65 - Abstract
Leucine dehydrogenase (LDH) is a NAD+-dependent oxidoreductase, which can selectively catalyze α-keto acids to obtain α-amino acids and their derivatives. It plays a key role in the biosynthesis of L-tert-leucine (L-Tle). As a non-naturally chiral amino acid, L-Tle can be used as an animal feed additive, nutrition fortifier, which is a perspective and important building block in the pharmaceutical, cosmetic, and food additive industry. In this study, four hypothetical leucine dehydrogenases were discovered by using genome mining technology, using the highly active leucine dehydrogenase LsLeuDH as a probe. These four leucine dehydrogenases were expressed in Escherichia coli BL21(DE3), respectively, and purified to homogeneity and characterized. Compared with the other enzymes, the specific activity of PfLeuDH also shows stronger advantage. In addition, the highly selective biosynthesis of L-Tle from trimethylpyruvic acid (TMP) was successfully carried out by whole-cell catalysis using engineered E. coli cells as biocatalyst, which can efficiently coexpress leucine dehydrogenase and formate dehydrogenase. One hundred-millimolar TMP was catalyzed for 25 h, and the yield and space-time yield of L-Tle reached 87.38% (e.e. >99.99%) and 10.90 g L–1 day–1. In short, this research has initially achieved the biosynthesis of L-Tle, laying a solid foundation for the realization of low-cost and large-scale biosynthesis of L-Tle.
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- 2021
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8. Cloning, expression, and polymorphism of the ECI1 gene in various pig breeds
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Yun-feng LU, Ji-bao CHEN, Bo ZHANG, Qing-gang LI, Zhi-xiu WANG, Hao ZHANG, and Ke-liang WU
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ECI1 gene ,lipid deposition ,mRNA expression ,pig ,single-nucleotide polymorphism ,Western blotting ,Agriculture (General) ,S1-972 - Abstract
Abstract: The enzyme Δ3,Δ2-dienoyl-CoA isomerase (ECI1) plays a crucial role in the mitochondrial β-oxidation of fatty acids with a double-bond in odd and even positions. The ECI1 gene might be a qualified candidate for studies pertaining to lipid deposition and meat quality in swine. In the present study, ECI1 cDNA of the Tibetan pig was obtained by in silico cloning and verified by PCR analysis. Single-nucleotide polymorphisms (SNPs) of ECI1 were screened by PCR-sequencing and genotypes of those SNPs were tested by PCR-restriction fragment length polymorphism (PCR-RFLP) in Diannan small-ear pigs (DSP, n=40), Tibetan pigs (TP, n=60) and Yorkshire pigs (YP, n=30). The expression levels of ECI1 were analyzed by real-time quantitative PCR and Western blotting in tissues of the liver, backfat, and longissimus dorsi (LD) muscle of DSP (n=8), TP (n=8) and YP (n=8). Single factor linear correlation analysis was applied separately for each breed to evaluate correlations between ECI1 gene expression in the LD muscle and intramuscular fat (IMF) content. We obtained an ECI1 gene length of 1 401 bp from the cDNA that contained a full coding region of 909 bp. Three novel SNPs (g.42425337G>A; g.42424666A>G; and g.42422755A>G) were detected, and only g.42424666A>G exhibited three genotypes among the three breeds. The ECI1 expression levels in the LD muscle of DSP and TP were significantly higher than that of YP (P
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- 2017
- Full Text
- View/download PDF
9. Dynamic Change of Genetic Diversity in Conserved Populations with Different Initial Genetic Architectures
- Author
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Yun-feng LU, Hong-wei LI, Ke-liang WU, and Chang-xin WU
- Subjects
genetic diversity ,genetic architecture ,conservation ,simulation ,Agriculture (General) ,S1-972 - Abstract
Maintenance and management of genetic diversity of farm animal genetic resources (AnGR) is very important for biological, socioeconomical and cultural significance. The core concern of conservation for farm AnGR is the retention of genetic diversity of conserved populations in a long-term perspective. However, numerous factors may affect evolution of genetic diversity of a conserved population. Among those factors, the genetic architecture of conserved populations is little considered in current conservation strategies. In this study, we investigated the dynamic changes of genetic diversity of conserved populations with two scenarios on initial genetic architectures by computer simulation in which thirty polymorphic microsatellite loci were chosen to represent genetic architecture of the populations with observed heterozygosity (Ho) and expected heterozygosity (He), observed and mean effective number of alleles (Ao and Ae), number of polymorphic loci (NP) and the percentage of polymorphic loci (PP), number of rare alleles (RA) and number of non-rich polymorphic loci (NRP) as the estimates of genetic diversity. The two scenarios on genetic architecture were taken into account, namely, one conserved population with same allele frequency (AS) and another one with actual allele frequency (AA). The results showed that the magnitude of loss of genetic diversity is associated with genetic architecture of initial conserved population, the amplitude of genetic diversity decline in the context AS was more narrow extent than those in context AA, the ranges of decline of Ho and Ao were about 4 and 2 times in AA compared with that in AS, respectively, the occurrence of first monomorphic locus and the time of change of measure NP in scenario AA is 20 generations and 23 generations earlier than that in scenario AS, respectively. Additionally, we found that NRP, a novel measure proposed by our research group, was a proper estimate for monitoring the evolution of genetic diversity in a closed conserved population. Our study suggested that current managements of conserved populations should emphasize on initial genetic architecture in order to make an effective and feasible conservation scheme.
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- 2013
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10. Porous copper cluster-based MOF with strong cuprophilic interactions for highly selective electrocatalytic reduction of CO2 to CH4
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Long-Zhang Dong, Yun-Feng Lu, Rui Wang, Jie Zhou, Yu Zhang, Lei Zhang, Jiang Liu, Shun-Li Li, and Ya-Qian Lan
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General Materials Science ,Electrical and Electronic Engineering ,Condensed Matter Physics ,Atomic and Molecular Physics, and Optics - Published
- 2022
11. Predesign of Catalytically Active Sites via Stable Coordination Cluster Model System for Electroreduction of CO 2 to Ethylene
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Yi-Rong Wang, Jing-Jing Liu, Ya-Qian Lan, Yu Zhang, Long-Zhang Dong, Lei Zhang, Yun-Feng Lu, Ru-Xin Yang, Jiang Liu, and Shun-Li Li
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Ethylene ,Molecular model ,chemistry.chemical_element ,General Chemistry ,General Medicine ,Electrocatalyst ,Copper ,Catalysis ,chemistry.chemical_compound ,Crystallography ,chemistry ,Cluster (physics) ,Molecule ,Faraday efficiency - Abstract
Purposefully designing the well-defined catalysts for the selective electroreduction of CO2 to C2 H4 is an extremely important but challenging work. In this work, three crystalline trinuclear copper clusters (Cu3 -X, X=Cl- , Br- , NO3- ) have been designed, containing three active Cu sites with the identical coordination environment and appropriate spatial distance, delivering high selectivity for the electrocatalytic reduction of CO2 to C2 H4 . The highest faradaic efficiency of Cu3 -X for CO2 -to-C2 H4 conversion can be adjusted from 31.90 % to 55.01 % by simply replacing the counter anions (NO3- , Cl- , Br- ). The DFT calculation results verify that Cu3 -X can facilitate the C-C coupling of identical *CHO intermediates, subsequently forming molecular symmetrical C2 H4 product. This work provides an important molecular model system and a new design perspective for electroreduction of CO2 to C2 products with symmetrical molecular structure.
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- 2021
12. A well-defined dual Mn-site based metal–organic framework to promote CO2 reduction/evolution in Li–CO2 batteries
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Xin Huang, Jian-Hui Wang, Lei Zhang, Jiang Liu, Shun-Li Li, Ya-Qian Lan, Yu Zhang, Yun-Feng Lu, and Long-Zhang Dong
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Battery (electricity) ,Materials science ,Metals and Alloys ,General Chemistry ,Pyrazole ,Decomposition ,Catalysis ,Cathode ,Surfaces, Coatings and Films ,Electronic, Optical and Magnetic Materials ,law.invention ,Hysteresis ,chemistry.chemical_compound ,chemistry ,Chemical engineering ,law ,Materials Chemistry ,Ceramics and Composites ,Metal-organic framework ,Low voltage - Abstract
A series of Li–CO2 battery cathode materials are reported based on metal–organic frameworks with dual-metal sites containing a metalloporphyrin and a metal-coordinated pyrazole. MnTPzP-Mn demonstrates a low voltage hysteresis of 1.05 V at 100 mA g−1 and good stability of 90 cycles at 200 mA g−1. Among them, the Mn-coordinated pyrazole site can promote the effective decomposition of Li2CO3, and the Mn-metalloporphyrin site contributes to the activation of CO2. This is the first example of using a crystalline cathode material with a well-defined structure to reveal natural catalytic sites for CO2 reduction/evolution reactions under aprotic conditions in Li–CO2 batteries.
- Published
- 2021
13. Improving Catalytic Performance of Lactobacillus Harbinensis D-Mandelate Dehydrogenase Toward D-O-Chloromandelic Acid by Laboratory Evolution
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Cunduo Tang, Xiang Zhang, Hong-Ling Shi, Xin-Xin Liu, Hong-Yan Wang, Yun-Feng Lu, Si-Pu Zhang, Yun-Chao Kan, and Lun-Guang Yao
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History ,Polymers and Plastics ,Business and International Management ,Industrial and Manufacturing Engineering - Published
- 2022
14. Predesign of Catalytically Active Sites via Stable Coordination Cluster Model System for Electroreduction of CO
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Yun-Feng, Lu, Long-Zhang, Dong, Jiang, Liu, Ru-Xin, Yang, Jing-Jing, Liu, Yu, Zhang, Lei, Zhang, Yi-Rong, Wang, Shun-Li, Li, and Ya-Qian, Lan
- Abstract
Purposefully designing the well-defined catalysts for the selective electroreduction of CO
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- 2021
15. A well-defined dual Mn-site based metal-organic framework to promote CO
- Author
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Long-Zhang, Dong, Yu, Zhang, Yun-Feng, Lu, Lei, Zhang, Xin, Huang, Jian-Hui, Wang, Jiang, Liu, Shun-Li, Li, and Ya-Qian, Lan
- Abstract
A series of Li-CO2 battery cathode materials are reported based on metal-organic frameworks with dual-metal sites containing a metalloporphyrin and a metal-coordinated pyrazole. MnTPzP-Mn demonstrates a low voltage hysteresis of 1.05 V at 100 mA g-1 and good stability of 90 cycles at 200 mA g-1. Among them, the Mn-coordinated pyrazole site can promote the effective decomposition of Li2CO3, and the Mn-metalloporphyrin site contributes to the activation of CO2. This is the first example of using a crystalline cathode material with a well-defined structure to reveal natural catalytic sites for CO2 reduction/evolution reactions under aprotic conditions in Li-CO2 batteries.
- Published
- 2021
16. Expression of Novel
- Author
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Yuan-Yuan, Jia, Yu-Li, Xie, Lu-Lu, Yang, Hong-Ling, Shi, Yun-Feng, Lu, Si-Pu, Zhang, Cun-Duo, Tang, Lun-Guang, Yao, and Yun-Chao, Kan
- Subjects
leucine dehydrogenase ,feed additive ,biocatalysis ,expression ,Bioengineering and Biotechnology ,formate dehydrogenase ,Original Research - Abstract
Leucine dehydrogenase (LDH) is a NAD+-dependent oxidoreductase, which can selectively catalyze α-keto acids to obtain α-amino acids and their derivatives. It plays a key role in the biosynthesis of L-tert-leucine (L-Tle). As a non-naturally chiral amino acid, L-Tle can be used as an animal feed additive, nutrition fortifier, which is a perspective and important building block in the pharmaceutical, cosmetic, and food additive industry. In this study, four hypothetical leucine dehydrogenases were discovered by using genome mining technology, using the highly active leucine dehydrogenase LsLeuDH as a probe. These four leucine dehydrogenases were expressed in Escherichia coli BL21(DE3), respectively, and purified to homogeneity and characterized. Compared with the other enzymes, the specific activity of PfLeuDH also shows stronger advantage. In addition, the highly selective biosynthesis of L-Tle from trimethylpyruvic acid (TMP) was successfully carried out by whole-cell catalysis using engineered E. coli cells as biocatalyst, which can efficiently coexpress leucine dehydrogenase and formate dehydrogenase. One hundred-millimolar TMP was catalyzed for 25 h, and the yield and space-time yield of L-Tle reached 87.38% (e.e. >99.99%) and 10.90 g L–1 day–1. In short, this research has initially achieved the biosynthesis of L-Tle, laying a solid foundation for the realization of low-cost and large-scale biosynthesis of L-Tle., Graphical Abstract Genome mining of Leucine dehydrogenase and one-pot synthesis of L-tert-leucine from trimethylpyruvic acid.
- Published
- 2021
17. Process Optimization of Ethanol Production from Propionic-Acid Pretreated Wheat Straw by Plackett-Burman Design Combined with Box-Behnken Response Surface Methodology with Immobilized Yeast
- Author
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Yuan Lingxiang, Yun-Feng Lu, and Long Tian
- Subjects
Biomaterials ,Plackett–Burman design ,Renewable Energy, Sustainability and the Environment ,Chemistry ,Bioengineering ,Ethanol fuel ,Process optimization ,Response surface methodology ,Straw ,Pulp and paper industry ,Box–Behnken design ,Yeast - Published
- 2018
18. Directed evolution of formate dehydrogenase and its application in the biosynthesis of L-phenylglycine from phenylglyoxylic acid
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Yun-Feng Lu, Fang-Hui Bai, Zhen-Hua Zhang, Tian-Tian Yang, Yao Lunguang, Tang Cunduo, Yu-Li Xie, Si-Pu Zhang, Hong-Ling Shi, and Kan Yunchao
- Subjects
Phenylglyoxylic acid ,biology ,Stereochemistry ,Process Chemistry and Technology ,NADH regeneration ,Dehydrogenase ,Formate dehydrogenase ,Leucine dehydrogenase ,Catalysis ,Cofactor ,chemistry.chemical_compound ,chemistry ,biology.protein ,Formate ,NAD+ kinase ,Physical and Theoretical Chemistry - Abstract
Formate dehydrogenase (FDH) is a d -2‑hydroxy acid dehydrogenase, and catalyzes the oxidation of formate to carbon dioxide, coupled with reduction of NAD+ to NADH that plays a key role in the process of NADH regeneration. In order to obtain high activity formate dehydrogenase mutants, the formate dehydrogenase CbFDHC23S was used as the parent to conduct two rounds of directed evolution, and a mutant M2 was obtained which specific activity was about 4 times more than the parent and was more suitable for coenzyme regeneration under physiological conditions. Then, the molecular mechanism of temperature characteristic and catalytic efficiency change was preliminarily elucidated by computer-aided method. Finally, an engineered E. coli strain was established to co-express formate dehydrogenase and l -leucine dehydrogenase and enantioselectively transform phenylglyoxylic acid to give l - phenylglycine (e.e. >99%), the yield and space-time yield of l - phenylglycine can reach 90.46% and 82.07 g·L−1·d−1. This study laid a theoretical foundation for the green biosynthesis of food additives such as chiral alcohols and amino acid derivatives catalyzed by FDH coupling to enhance the regeneration capacity of NADH, reduce the regeneration cost of NADH, and achieve high efficiency and low cost.
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- 2021
19. Cloning, expression, and polymorphism of the ECI1 gene in various pig breeds
- Author
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Qing-gang Li, Hao Zhang, Yun-feng Lu, Bo Zhang, Ji-bao Chen, Zhixiu Wang, and Ke-liang Wu
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pig ,0301 basic medicine ,lipid deposition ,Agriculture (General) ,Single-nucleotide polymorphism ,Plant Science ,Biology ,Molecular cloning ,Biochemistry ,Western blotting ,S1-972 ,03 medical and health sciences ,Food Animals ,Complementary DNA ,Gene expression ,Genotype ,Gene ,Ecology ,mRNA expression ,single-nucleotide polymorphism ,ECI1 gene ,Molecular biology ,030104 developmental biology ,Real-time polymerase chain reaction ,Animal Science and Zoology ,Intramuscular fat ,Agronomy and Crop Science ,Food Science - Abstract
The enzyme Δ3,Δ2-dienoyl-CoA isomerase (ECI1) plays a crucial role in the mitochondrial β-oxidation of fatty acids with a double-bond in odd and even positions. The ECI1 gene might be a qualified candidate for studies pertaining to lipid deposition and meat quality in swine. In the present study, ECI1 cDNA of the Tibetan pig was obtained by in silico cloning and verified by PCR analysis. Single-nucleotide polymorphisms (SNPs) of ECI1 were screened by PCR-sequencing and genotypes of those SNPs were tested by PCR-restriction fragment length polymorphism (PCR-RFLP) in Diannan small-ear pigs (DSP, n=40), Tibetan pigs (TP, n=60) and Yorkshire pigs (YP, n=30). The expression levels of ECI1 were analyzed by real-time quantitative PCR and Western blotting in tissues of the liver, backfat, and longissimus dorsi (LD) muscle of DSP (n=8), TP (n=8) and YP (n=8). Single factor linear correlation analysis was applied separately for each breed to evaluate correlations between ECI1 gene expression in the LD muscle and intramuscular fat (IMF) content. We obtained an ECI1 gene length of 1 401 bp from the cDNA that contained a full coding region of 909 bp. Three novel SNPs (g.42425337G>A; g.42424666A>G; and g.42422755A>G) were detected, and only g.42424666A>G exhibited three genotypes among the three breeds. The ECI1 expression levels in the LD muscle of DSP and TP were significantly higher than that of YP (P
- Published
- 2017
20. Design and research of underwater hydraulic driven diamond chain saw cutting machine
- Author
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Yun-Feng Lu, Zhi-Ming Wang, Shao-Rong Yang, and Yue Yu
- Subjects
Materials science ,Cutting tool ,Tension (physics) ,engineering ,Diamond ,Mechanical engineering ,Metre ,Rotational speed ,Edge (geometry) ,engineering.material ,Underwater ,Dynamic load testing - Abstract
The hydraulic driven chain saw is a cutting tool for underwater operation. The hydraulic driven chain saw was designed, the hydraulic adjusting device was designed, the hydraulic chain saw experimental platform was designed, the sawing efficiency and power consumption experiments were carried out, and the diamond saw chain wear experiment was carried out. The experimental results show that:When cutting C25 standard reinforced concrete floor plate, the tension of tight edge of diamond saw chain is large; the dynamic load of diamond saw chain has the greatest influence on the tension of diamond saw chain, and the dynamic load is proportional to the rotation speed of chain wheel, the pitch of diamond saw chain, the weight per meter of diamond saw chain and the length of tight edge, among which the rotation speed of chain wheel has an effect on it The noise is the largest; the power consumption of cutting concrete is 2.85kw, the efficiency of sawing concrete is 0.93m2/h, and the sawing area of diamond saw chain per meter is 16.7m2/m.
- Published
- 2021
21. The composition, pharmacological effects, related mechanisms and drug delivery of alkaloids from Corydalis yanhusuo
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Jia-hua Feng, Kang Chen, Si-yu Shen, Yun-feng Luo, Xi-hong Liu, Xin Chen, Wei Gao, and Yu-ru Tong
- Subjects
Corydalis yanhusuo ,Papaveraceae ,Alkaloids ,Pharmacological effects ,Traditional Chinese medicine ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Corydalis yanhusuo W. T. Wang, also known as yanhusuo, yuanhu, yanhu and xuanhu, is one of the herb components of many Chinese Traditional Medicine prescriptions such as Jin Ling Zi San and Yuanhu-Zhitong priscription. C. yanhusuo was traditionally used to relieve pain and motivate blood and Qi circulation. Now there has been growing interest in pharmacological effects of alkaloids, the main bioactive components of C. yanhusuo. Eighty-four alkaloids isolated from C. yanhusuo are its important bioactive components and can be characterized into protoberberine alkaloids, aporphine alkaloids, opiate alkaloids and others and proper extraction or co-administration methods modulate their contents and efficacy. Alkaloids from C. yanhusuo have various pharmacological effects on the nervous system, cardiovascular system, cancer and others through multiple molecular mechanisms such as modulating neurotransmitters, ion channels, gut microbiota, HPA axis and signaling pathways and are potential treatments for many diseases. Plenty of novel drug delivery methods such as autologous red blood cells, self-microemulsifying drug delivery systems, nanoparticles and others have also been investigated to better exert the effects of alkaloids from C. yanhusuo. This review summarized the alkaloid components of C. yanhusuo, their pharmacological effects and mechanisms, and methods of drug delivery to lay a foundation for future investigations.
- Published
- 2023
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- View/download PDF
22. [Community structure and leaf trait diversity in a vulnerable species, Phoebe chekiangensis (Lauraceae).]
- Author
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Yun Feng, Lu, Nan Cai, Pei, Ya Jun, Zhu, Zhi Liang, Bai, An Na, Yang, Jun Hong, Zhang, Lu Huan, Lou, and Zai Kang, Tong
- Subjects
Plant Leaves ,China ,Lauraceae ,Phenotype ,Climate Change ,Genetic Variation ,Biodiversity - Abstract
Phoebe chekiangensis, as one original species of 'Nanmu with golden tint', harbors limited natural distribution regions, which is recognized as a vulnerable species in China under nationwide protection. Under the background of global climate change, it is of scientific significance to study the community structure and diversity of the natural populations of P. chekiangensis. In this study, community structure, species diversity and the phenotypic variation leaf traits in the communities of thirteen residue natural populations were analyzed. A total of 235 vascular species from 162 genera and 87 families were found in 16 plots within 13 natural populations, including 221 seed plants from 151 genera and 79 families. The species diversity in tree layer of community in Kaihua, Zhejiang and Linan, Zhejiang was significantly lower than that of other communities due to severe disturbance, while intermediate disturbance promoted the species diversity of shrub layer in community in Jianning, Fujian, and slight disturbance was beneficial to natural regeneration of P. chekiangensis. Foliage phenotype was important for species identification within Phoebe genus. There existed substantial variation in foliage phenotypic diversity among and within populations. The mean coefficient of variation was 17.2%, ranging from 10.4% to 27.5%. The variation was greater among populations (53.6%) than within populations (17.0%). Mean phenotypic differentiation coefficient was 75.1% among populations, indicating that the variance among populations was the main source for the phenotypic variation of the species. Results from the cluster analysis indicated that the 13 natural populations were divided into two distinct groups based on the Euclidean distance (10 cm), with stochastic variation.浙江楠为商品材“金丝楠木”的原植物之一,天然分布区窄,属国家二级保护渐危种,在全球气候变化背景下,研究其天然种群所在森林群落的结构特征及其多样性具有重要意义.本研究以浙江楠天然分布区内13个典型种群为对象,研究其所在群落的群落结构特征、物种多样性和叶片表型变异特征.结果表明: 浙江楠天然种群所在群落的结构复杂,物种多样性较高,13个群落的16块样地内共有维管束植物87科162属235种,其中种子植物79科151属221种;重度干扰导致浙江开化、浙江临安等群落的乔木层物种多样性指数显著低于其他群落,而中度干扰提高了福建建宁群落灌木层物种多样性,轻度干扰则利于浙江楠种群自然更新.叶片表型作为楠木属植物重要的分类依据,叶片表型性状在种群间、种群内均存在丰富变异,平均变异系数为17.2%,变异幅度为10.4%~27.5%.种群间的变异(53.6%)大于种群内的变异(17.0%),平均表型分化系数为75.1%,种群间变异是浙江楠叶片表型变异的主要来源.基于欧式距离(10 cm)将13个天然种群划分为两大类群,但变异呈随机性.
- Published
- 2018
23. Microbial Oligo‐α‐1,6‐Glucosidase: Current Developments and Future Perspectives
- Author
-
Yun-Feng Lu, Tang Cunduo, Dong Zixing, Kan Yunchao, and Lunguang Yao
- Subjects
Biochemistry ,Chemistry ,Organic Chemistry ,Current (fluid) ,Food Science - Published
- 2019
24. Cyberlindnera xishuangbannaensis f.a., sp. nov., a yeast isolated from rotting wood
- Author
-
Xiao-Jing Liu, Yun-Feng Lu, Jun Zheng, and Feng-Li Hui
- Subjects
0301 basic medicine ,China ,Rainforest ,Phylogenetic tree ,Strain (chemistry) ,Species name ,MycoBank ,General Medicine ,Sequence Analysis, DNA ,Ribosomal RNA ,Biology ,Microbiology ,Wood ,Yeast ,03 medical and health sciences ,030104 developmental biology ,Botany ,DNA, Ribosomal Spacer ,Saccharomycetales ,Internal transcribed spacer ,DNA, Fungal ,Mycological Typing Techniques ,Gene ,Ecology, Evolution, Behavior and Systematics ,Phylogeny - Abstract
Three strains representing a novel species of yeast were isolated from samples of rotting wood collected from Xishuangbanna Tropical Rainforest in Yunnan Province, PR China. Phylogenetic analysis based on the concatenated sequences of the internal transcribed spacer (ITS) region and the D1/D2 domains of the large subunit (LSU) rRNA gene revealed that the novel species is a member of the genus Cyberlindnera, although the formation of ascospores was not observed. The novel species was related most closely to the type strain of the species Candida pattaniensis, but they had a 0.5 % sequence divergence (3 substitutions, 0 gap) in the D1/D2 domain and a 5.4 % sequence divergence (21 substitutions, 10 gaps) in the ITS region. The novel species could also be differentiated from the closely related species by some biochemical and physiological characteristics. The species name Cyberlindnera xishuangbannaensis f.a., sp. nov. is proposed to accommodate these strains, with NYNU 16752T (=CICC 33163T=CBS 14692T) designated as the type strain. The MycoBank number is MB 822199.
- Published
- 2017
25. Ogataea neixiangensis sp. nov. and Ogataea paraovalis f.a., sp. nov., two methanol-assimilating yeast species isolated from rotting wood
- Author
-
Min Wang, Jun Zheng, Yun-Feng Lu, and Feng-Li Hui
- Subjects
0301 basic medicine ,China ,030106 microbiology ,Central china ,Biology ,Microbiology ,03 medical and health sciences ,Intergenic region ,RNA, Ribosomal, 16S ,Botany ,Internal transcribed spacer ,DNA, Fungal ,Mycological Typing Techniques ,Ecology, Evolution, Behavior and Systematics ,Phylogeny ,MycoBank ,Methanol ,General Medicine ,Sequence Analysis, DNA ,Ribosomal RNA ,Spores, Fungal ,Wood ,Yeast ,Spore ,RNA, Ribosomal ,Saccharomycetales ,Taxonomy (biology) - Abstract
Four yeast strains were isolated from rotting wood samples collected in the Baotianman Nature Reserve in Henan Province, central China. The sequences of the D1/D2 domains of the large subunit rRNA gene and the internal transcribed spacer regions showed that these four strains represent two different undescribed yeast species belonging to the Ogataea clade. Ogataea neixiangensis sp. nov. produces two to four hat-shaped ascospores per ascus, and its closest relative among recognized species is Candida nitratophila. Ogataea paraovalis f.a., sp. nov. is closely related to Candida ovalis but the formation of ascospores was not observed on various sporulation media. The type strain of O. neixiangensis sp. nov. (MycoBank number MB 820697) is NYNU 16951T (=CICC 33166T=CBS 14695T), and the type strain of O. paraovalis f.a., sp. nov. (MycoBank number MB 820698) is NYNU 167106T (=CICC 33168T=CBS 14697T).
- Published
- 2017
26. Dynamic Change of Genetic Diversity in Conserved Populations with Different Initial Genetic Architectures
- Author
-
Chang-xin Wu, Ke-liang Wu, Yun-feng Lu, and Hong-wei Li
- Subjects
Conservation genetics ,Genetics ,Genetic diversity ,education.field_of_study ,Ecology ,Agriculture (General) ,Population ,conservation ,genetic diversity ,Plant Science ,Biology ,simulation ,genetic architecture ,Biochemistry ,Genetic architecture ,S1-972 ,Food Animals ,Genetic distance ,Animal Science and Zoology ,Genetic variability ,Gene pool ,Allele ,education ,Agronomy and Crop Science ,Food Science - Abstract
Maintenance and management of genetic diversity of farm animal genetic resources (AnGR) is very important for biological, socioeconomical and cultural significance. The core concern of conservation for farm AnGR is the retention of genetic diversity of conserved populations in a long-term perspective. However, numerous factors may affect evolution of genetic diversity of a conserved population. Among those factors, the genetic architecture of conserved populations is little considered in current conservation strategies. In this study, we investigated the dynamic changes of genetic diversity of conserved populations with two scenarios on initial genetic architectures by computer simulation in which thirty polymorphic microsatellite loci were chosen to represent genetic architecture of the populations with observed heterozygosity (Ho) and expected heterozygosity (He), observed and mean effective number of alleles (Ao and Ae), number of polymorphic loci (NP) and the percentage of polymorphic loci (PP), number of rare alleles (RA) and number of non-rich polymorphic loci (NRP) as the estimates of genetic diversity. The two scenarios on genetic architecture were taken into account, namely, one conserved population with same allele frequency (AS) and another one with actual allele frequency (AA). The results showed that the magnitude of loss of genetic diversity is associated with genetic architecture of initial conserved population, the amplitude of genetic diversity decline in the context AS was more narrow extent than those in context AA, the ranges of decline of Ho and Ao were about 4 and 2 times in AA compared with that in AS, respectively, the occurrence of first monomorphic locus and the time of change of measure NP in scenario AA is 20 generations and 23 generations earlier than that in scenario AS, respectively. Additionally, we found that NRP, a novel measure proposed by our research group, was a proper estimate for monitoring the evolution of genetic diversity in a closed conserved population. Our study suggested that current managements of conserved populations should emphasize on initial genetic architecture in order to make an effective and feasible conservation scheme.
- Published
- 2013
27. [Study of Retrieving the Sandy Surface Roughness Land Based on the Bidirectional Reflectance Model]
- Author
-
Yun-feng, Lu and Yun-sheng, Zhao
- Abstract
In the semiarid area, the structural nature of sandy land is changed due to wind erosion. Furthermore, assessing the changes in the composition and surface roughness in several spatial and temporal scales is significant for the wind erosion model calculations. As a noninvasive approach, remote sensing can be used to improve the study of sandy surface in time and space. In order to characterize the surface structure using the reflectance of sandy land, we analysis the effects of the changes of sandy surface structure on the bidirectional reflectance distribution basing on the multi-angular and hyperspectral measurements in the field; the measured sandy land samples are taken from nature, but the surface structures are artificial: one type is the direction of sand furrows is parallel to the incident direction, the other is the direction of sand furrows is perpendicular to the incident direction. At the same time, we analyzed the effects of surface structure on the bidirectional reflectance factor of sand land and we also retrieve the sandy surface roughness using the reflectance model parameter. The results suggest that both types of sand furrows will influence the distribution of reflectance of sandy land surface, for example, the backward scattering of sandy land increased when the direction of sand furrows is perpendicular to the incident direction, and the range of backward scattering of sandy land expended when the direction of sand furrows is parallel to the incident direction. When we compared the measured reflectance with the modeled results basing on the reflectance model, it is found that the reflectance model can be used to simulate the reflectance property of sandy land surface and prove that the parameter of model is useful for retrieving the surface roughness. This research not only presents the sample for quantifying the structural information of sandy land by the reflectance measurements, but also shows valuable reference for the research of intrinsic optical property of sandy land and the reversion of the texture of sandy land. In other words, this paper can also help the scientists understand the effect of the structural information on the optical properties of sandy land.
- Published
- 2016
28. Study on Cold Extrusion Technology of 20Cr Bevel Gears
- Author
-
Yun Feng Lu and Shao Rong Yang
- Subjects
Engineering ,business.product_category ,business.industry ,Mold ,General Engineering ,medicine ,Bevel gear ,Mechanical engineering ,Extrusion ,medicine.disease_cause ,business - Abstract
In this paper we present the cold extrusion shaping technology of 20Cr bevel gears. First, we analyzed a series of processing and treatment referring to bevel gear cold extrusion technology and calculated the cold extrusion pressures used for mold design; then, we designed and cold extruded two- or three-ring composite cold extrusion dies with its precise guiding device; and last, we experimentally tested our designed and extruded bevel gear. The results showed that 20Cr bevel gears shaped with cold extrusion have qualified properties and satisfy application requirements within the required error range. This research is of significance in actual cold extrusion production.
- Published
- 2012
29. Application of Functional Nanomaterials in Food Safety
- Author
-
Hua-Qin Chu and Yun-Feng Lu
- Subjects
Chemistry ,business.industry ,Nanotechnology ,General Medicine ,Food safety ,business ,Nanomaterials - Published
- 2010
30. Astragaloside alleviates alcoholic fatty liver disease by suppressing oxidative stress
- Author
-
Zhi‐Bin Jiang, Jie Gao, Yi‐Hui Chai, Wen Li, Yun‐Feng Luo, and Yun‐Zhi Chen
- Subjects
alcoholic fatty liver disease ,astragaloside ,lipid accumulation ,oxidative stress ,Medicine (General) ,R5-920 - Abstract
Abstract Alcoholic fatty liver disease (AFLD) is the most common liver disease and can progress to fatal liver cirrhosis and carcinoma, affecting millions of patients worldwide. The functions of astragaloside on the cardiovascular system have been elucidated. However, its role in AFLD is unclear. Ethanol‐treated AML‐12 cells were used as a cell model of alcoholic fatty liver. Real‐time quantitative reverse transcription‐PCR and Western blotting detected genes and proteins expressions. Reactive oxygen species (ROS), triglyceride, total cholesterol, low‐density lipoprotein, albumin, ferritin, bilirubin, superoxide dismutase, aspartate aminotransferase (AST), and alanine aminotransferase (ALT) were examined using commercial kits. Lipid accumulation was assessed by Oil red O staining. MTT and flow cytometry measured cell viability and apoptosis. JC‐1 was used to analyze mitochondrial membrane potential. A rat model of AFLD was established by treating rats with ethanol. Astragaloside suppressed ethanol‐induced lipid accumulation, oxidative stress, and the production of AST and ALT in AML‐12 cells. Ethanol induced TNF‐α and reduced IL‐10 expression, which were reversed by astragaloside. Ethanol promoted Bax expression and cytochrome C release and inhibited Bcl‐2 and ATP expression. Astragaloside hampered these apoptosis effects in AML‐12 cells. Impaired mitochondrial membrane potential was recovered by astragaloside. However, all these astragaloside‐mediated beneficial effects were abolished by the ROS inducer pyocyanin. Ethanol‐induced activation of NF‐κB signaling was suppressed by astragaloside in vitro and in vivo, suggesting that astragaloside inhibited oxidative stress by suppressing the activation of NF‐κB signaling, thus improving liver function and alleviating AFLD in rats. Our study elucidates the pharmacological mechanism of astragaloside and provides potential therapeutic strategies for AFLD.
- Published
- 2021
- Full Text
- View/download PDF
31. A New Constructive Cost Model for Software Testing Project Management
- Author
-
Yong-feng Yin and Yun-feng Lu
- Subjects
Software development process ,Test strategy ,Computer science ,business.industry ,Software construction ,Systems engineering ,Software development ,Software performance testing ,Software verification and validation ,Software engineering ,business ,System integration testing ,Software project management - Abstract
Software testing is becoming a relatively independent activity of software development process. To estimate the cost of software testing is an important part of project management. The researchers have focused on the software cost and treated software testing cost as a part of the total software development cost for several years. Few researchers consider software testing as a separate process to estimate its’ cost. This paper proposed a new constructive cost model for software testing project management (CCMST) which contains the main cost divers of software testing. The cost drivers included in this model is more comprehensive than former models. Application case study proved the model is usable and valid.
- Published
- 2013
32. Whole-genome resequencing reveals candidate mutations for pig prolificacy.
- Author
-
Wen-Ting Li, Meng-Meng Zhang, Qi-Gang Li, Hui Tang, Li-Fan Zhang, Ke-Jun Wang, Mu-Zhen Zhu, Yun-Feng Lu, Hai-Gang Bao, Yuan-Ming Zhang, Qiu-Yan Li, Ke-Liang Wu, and Chang-Xin Wu
- Subjects
NUCLEOTIDE sequencing ,GENETIC mutation ,SWINE ,MAMMAL fertility ,BIOLOGICAL variation ,MORPHOGENESIS - Abstract
Changes in pig fertility have occurred as a result of domestication, but are not understood at the level of genetic variation. To identify variations potentially responsible for prolificacy, we sequenced the genomes of the highly prolific Taihu pig breed and four control breeds. Genes involved in embryogenesis and morphogenesis were targeted in the Taihu pig, consistent with the morphological differences observed between the Taihu pig and others during pregnancy. Additionally, excessive functional non-coding mutations have been specifically fixed or nearly fixed in the Taihu pig.We focused attention on an oestrogen response element (ERE) within the first intron of the bone morphogenetic protein receptor type-1B gene (BMPR1B) that overlaps with a known quantitative trait locus (QTL) for pig fecundity. Using 242 pigs from 30 different breeds, we confirmed that the genotype of the ERE was nearly fixed in the Taihu pig. ERE function was assessed by luciferase assays, examination of histological sections, chromatin immunoprecipitation, quantitative polymerase chain reactions, and western blots. The results suggest that the ERE may control pig prolificacy via the cis-regulation of BMPR1B expression. This study provides new insight into changes in reproductive performance and highlights the role of non-coding mutations in generating phenotypic diversity between breeds. [ABSTRACT FROM AUTHOR]
- Published
- 2017
- Full Text
- View/download PDF
33. A non-labeled DNA biosensor based on light addressable potentiometric sensor modified with TiO2 thin film
- Author
-
Xiaolin Zong, Yun-feng Lu, Ping Wang, Xiaoling Wu, and Chunsheng Wu
- Subjects
Light ,Ultraviolet Rays ,Inorganic chemistry ,Analytical chemistry ,Biosensing Techniques ,Light-addressable potentiometric sensor ,medicine.disease_cause ,General Biochemistry, Genetics and Molecular Biology ,chemistry.chemical_compound ,medicine ,General Pharmacology, Toxicology and Pharmaceutics ,Thin film ,Oligonucleotide Array Sequence Analysis ,chemistry.chemical_classification ,Titanium ,General Veterinary ,Propylamines ,Chemistry ,Biomolecule ,Hydrolysis ,Nucleic Acid Hybridization ,General Medicine ,Equipment Design ,Silanes ,Oxygen ,Covalent bond ,Silanization ,Titanium dioxide ,Potentiometry ,Fluorescein ,Biosensor ,Ultraviolet ,Fluorescein-5-isothiocyanate ,Biotechnology - Abstract
Titanium dioxide (TiO(2)) thin film was deposited on the surface of the light addressable potentiometric sensor (LAPS) to modify the sensor surface for the non-labeled detection of DNA molecules. To evaluate the effect of ultraviolet (UV) treatment on the silanization level of TiO(2) thin film by 3-aminopropyltriethoxysilane (APTS), fluorescein isothiocyanate (FITC) was used to label the amine group on the end of APTS immobilized onto the TiO(2) thin film. We found that, with UV irradiation, the silanization level of the irradiated area of the TiO(2) film was improved compared with the non-irradiated area under well-controlled conditions. This result indicates that TiO(2) can act as a coating material on the biosensor surface to improve the effect and efficiency of the covalent immobilization of biomolecules on the sensor surface. The artificially synthesized probe DNA molecules were covalently linked onto the surface of TiO(2) film. The hybridization of probe DNA and target DNA was monitored by the recording of I-V curves that shift along the voltage axis during the process of reaction. A significant LAPS signal can be detected at 10 micromol/L of target DNA sample.
- Published
- 2009
34. Metabolic Engineering of Saccharomyces cerevisiae for High-Level Friedelin via Genetic Manipulation
- Author
-
Hai-Yun Gao, Huan Zhao, Tian-Yuan Hu, Zhou-Qian Jiang, Meng Xia, Yi-Feng Zhang, Yun Lu, Yuan Liu, Yan Yin, Xiao-Chao Chen, Yun-Feng Luo, Jia-Wei Zhou, Jia-Dian Wang, Jie Gao, Wei Gao, and Lu-Qi Huang
- Subjects
friedelin ,triterpenes ,Saccharomyces cerevisiae ,genome ,CRISPR/Cas9 ,optimized medium ,Biotechnology ,TP248.13-248.65 - Abstract
Friedelin, the most rearranged pentacyclic triterpene, also exhibits remarkable pharmacological and anti-insect activities. In particular, celastrol with friedelin as the skeleton, which is derived from the medicinal plant Tripterygium wilfordii, is a promising drug due to its anticancer and antiobesity activities. Although a previous study achieved friedelin production using engineered Saccharomyces cerevisiae, strains capable of producing high-level friedelin have not been stably engineered. In this study, a combined strategy was employed with integration of endogenous pathway genes into the genome and knockout of inhibiting genes by CRISPR/Cas9 technology, which successfully engineered multiple strains. After introducing an efficient TwOSC1T502E, all strains with genetic integration (tHMG1, ERG1, ERG20, ERG9, POS5, or UPC2.1) showed a 3.0∼6.8-fold increase in friedelin production compared with strain BY4741. Through further double knockout of inhibiting genes, only strains GD1 and GD3 produced higher yields. Moreover, strains GQ1 and GQ3 with quadruple mutants (bts1; rox1; ypl062w; yjl064w) displayed similar increases. Finally, the dominant strain GQ1 with TwOSC1T502E was cultured in an optimized medium in shake flasks, and the final yield of friedelin reached 63.91 ± 2.45 mg/L, which was approximately 65-fold higher than that of the wild-type strain BY4741 and 229% higher than that in ordinary SD-His-Ura medium. It was the highest titer for friedelin production to date. Our work provides a good example for triterpenoid production in microbial cell factories and lays a solid foundation for the mining, pathway analysis, and efficient production of valuable triterpenoids with friedelin as the skeleton.
- Published
- 2022
- Full Text
- View/download PDF
35. Responsive Binary Composite Hydrogel Opals.
- Author
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Zhong-Wei Niu, Dan Li, Zhen-Zhong Yang, Zhi-Bing Hu, Yun-Feng Lu, and Charles C. Han
- Published
- 2003
- Full Text
- View/download PDF
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