1. 5-(3′,4′-Dihydroxyphenyl-γ-valerolactone), a Major Microbial Metabolite of Proanthocyanidin, Attenuates THP-1 Monocyte-Endothelial Adhesion
- Author
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Charles M.C. Lee, Jung Han Yoon Park, Ki Won Lee, Ji Seung Kim, Seung Min Han, Yun Sil Oh, Chang Yong Lee, and Jong Hun Kim
- Subjects
0301 basic medicine ,Cell Communication ,atherosclerosis prevention ,monocyte-endothelial adhesion ,Umbilical vein ,Monocytes ,lcsh:Chemistry ,chemistry.chemical_compound ,THP1 cell line ,proanthocyanidin microbial metabolite ,Phosphorylation ,Promoter Regions, Genetic ,lcsh:QH301-705.5 ,Spectroscopy ,5-(3′,4′-dihydroxyphenyl-γ-valerolactone) ,NF-kappa B ,Catechin ,General Medicine ,Computer Science Applications ,medicine.anatomical_structure ,Biochemistry ,Tumor necrosis factor alpha ,Metabolic Networks and Pathways ,Signal Transduction ,Vascular Cell Adhesion Molecule-1 ,Biology ,Protective Agents ,Catalysis ,Article ,Cell Line ,Inorganic Chemistry ,03 medical and health sciences ,medicine ,Cell Adhesion ,Humans ,Proanthocyanidins ,Physical and Theoretical Chemistry ,Cell adhesion ,Molecular Biology ,030109 nutrition & dietetics ,Tumor Necrosis Factor-alpha ,Monocyte ,Organic Chemistry ,Endothelial Cells ,IκBα ,030104 developmental biology ,lcsh:Biology (General) ,lcsh:QD1-999 ,chemistry ,Gene Expression Regulation ,Procyanidin A1 - Abstract
Several metabolomics of polymeric flavan-3-ols have reported that proanthocyanidins are extensively metabolized by gut microbiota. 5-(3′,4′-dihydroxyphenyl)-γ-valerolactone (DHPV) has been reported to be the major microbial metabolite of proanthocyanidins. We demonstrated that DHPV has stronger prevention effect on tumor necrosis factor (TNF)-α-stimulated adhesion of THP-1 human monocytic cells to human umbilical vein endothelial cells compared to its potential precursors such as procyanidin A1, A2, B1 and B2, (+)catechin, (−)epicatechin and its microbial metabolites such as 3-(3,4-dihydroxyphenyl)propionic acid and 2-(3,4-dihydroxyphenyl)acetic acid. Mechanism study showed that DHPV prevents THP-1 monocyte-endothelial cell adhesion by downregulating TNF-α-stimulated expressions of the two biomarkers of atherosclerosis such as vascular cell adhesion molecule-1 and monocyte chemotactic protein-1, activation of nuclear factor kappa B transcription and phosphorylation of I kappa-B kinase and IκBα. We suggested that DHPV has higher potentiality in prevention of atherosclerosis among the proanthocyanidin metabolites.
- Published
- 2017
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