1. Synergistic effects of loxoprofen and glycine on the micturition reflex in conscious rats
- Author
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Masaru Yoshizumi, Masahito Kawatani, Toshie Nimura, Mikako Saito, Yumiko Fukiya, and Kazumasa Matsumoto-Miyai
- Subjects
Consciousness ,media_common.quotation_subject ,Urinary system ,Glycine ,Urination ,Pharmacology ,Inhibitory postsynaptic potential ,General Biochemistry, Genetics and Molecular Biology ,Reflex ,medicine ,Animals ,media_common ,Micturition reflex ,medicine.diagnostic_test ,biology ,Dose-Response Relationship, Drug ,Phenylpropionates ,Chemistry ,Drug Administration Routes ,Cystometry ,Drug Synergism ,General Medicine ,Loxoprofen ,Rats ,biology.protein ,Female ,Cyclooxygenase ,medicine.drug - Abstract
We examined the inhibitory effects of loxoprofen, a cyclooxygenase inhibitor, and glycine, a major inhibitory neurotransmitter, on the micturition reflex in conscious rats and hypothesized that these drugs would interact synergistically to inhibit micturition. Voiding behaviors were assessed using a metabolic cage. Oral loxoprofen decreased the urinary frequency, and only a high dose(10 mg/kg) significantly reduced the voided volume. With cystometry, intravenous loxoprofen(0.1-3 mg/kg) and glycine (30 and 100 mg/kg) prolonged the intercontraction intervals (ICI) in adose-dependent manner, but did not change the maximum voiding pressure (MVP) in conscious rats. The combination of loxoprofen (3 mg/kg) and glycine (100 mg/kg) strongly prolonged the ICI more than with either drug alone. The lowest dose of loxoprofen (0.1 mg/kg) and glycine(30 mg/kg) did not affect either the ICI or the MVP, but their combination resulted in a significant increase in the ICI. These results suggest that the combined administration of loxoprofen and glycine produced a synergistic inhibitory effect on the micturition reflex.
- Published
- 2014