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In order to improve the accuracy of the evaluation results of multiperception intelligent wearable devices, the mathematical statistical characteristics based on speech, behavior, environment, and physical signs are proposed; first, the PCA feature compression algorithm was used to reduce the dimension of these features, and the differences among different training samples were compared and analyzed; then, three weak classifiers are designed using the logistic regression algorithm, and finally, a strong classifier with higher prediction accuracy is designed according to the boosting decision fusion method and ensemble learning idea. The results showed that the accuracy of the logistic regression model trained with the feature data of voice PCA was 0.964, but the recall rate and crossover results were significantly reduced to 0.844 and 0.846, respectively. The accuracy, accuracy and recall of the decision fusion model based on the boosting method and integrated learning are 0.969, and the prediction accuracy of K-folds cross-validation is also as high as 0.956; the superposition fusion results of three weak classifiers achieve a better classification effect.

Inflammation plays a critical role in the pathogenesis of endometriosis. We aimed to study the proinflammatory effect of Chemerin chemokine-like receptor 1 (CMKLR1) in patients with endometriosis. Sixty patients with endometriosis and 50 healthy controls were recruited in this study for the collection of endometrial samples and peritoneal fluid. The expression levels of CMKLR1, IL-6, MCP-1, and TNF-α in peritoneal fluid and endometrial tissues were detected by ELISA, qRT-PCR, and immunohistochemical staining. Human endometrial stromal cells (HESCs) were used to measure the Chemerin-induced CMKLR1 activation and inflammatory responses. CMKLR1 level was significantly up-regulated in peritoneal fluid and endometrial tissues in patients with endometriosis. Interestingly, CMKLR1 overexpression positively correlated with pro-inflammatory cytokines and chemokine in both peritoneal fluid and ectopic endometrium. Chemerin treatment increased the expression of CMKLR1, and aggravated inflammatory responses in HESCs. CMKLR1 is up-regulated in peritoneal fluid and endometrial tissues, and promotes the inflammatory responses in of endometriosis.

本文报道1例发生于新生儿的颈部巨大先天性纤维肉瘤。患儿男，第1胎，第1产，胎龄36+4周，单胎。因“出生后发现颈部巨大包块，气管插管辅助呼吸7 h”120急诊转入成都市妇女儿童中心医院。术前CT加三维重建查示：颈部正中巨大不规则低密度灶包块，大小12.0 cm×8.1 cm×7.1 cm，口咽明显受压闭塞，考虑脉管畸形、淋巴管畸形。在全身麻醉下行肿物全切除手术。病理检查（大体标本）示，肿瘤表面光滑，切面奶油状，术后病理诊断为间叶源性肿瘤，根据形态为婴儿先天性纤维肉瘤。术后家长放弃治疗自动出院，随访患儿于出院数天后死亡。.

Cardiac patches are biomaterials that can be used for transplantation and repair of damaged myocardium by combining seed cells with the ability to form cardiomyocytes and suitable scaffold materials. On the one hand, they provide temporary support to the infarcted area, and on the other hand, they repair the damaged myocardium by delivering cells or bioactive factors to integrate with the host, which have gradually become a hot research topic in recent years. This paper summarizes the structural properties of natural myocardium and reviews the recent research progress of cardiac patches, including the seed cells and scaffold materials used in patch preparation, as well as the main methods of scaffold preparation and the structure properties of various scaffolds. In addition, a comprehensive analysis of the problems faced in the clinical implementation of cardiac patches is presented. Finally, we look forward to the development of cardiac patches and point out that precisely tunable anisotropic tissue engineering scaffolds close to natural myocardial tissue will become an important direction for future research.

The association between periodontal disease and systemic disease has become a research hotspot.

Functional composite materials (FCMs) play an important role in many areas including the aerospace, and transportation industries as well as in domestic appliances. In this study, two types of FCMs were prepared using nanocarbon-coated glass fibers (NCGFs) and glass fiber cloths (NCGFCs) as reinforcements and epoxy resin as the matrix. Nanocarbon coatings of glass fiber cloths have good electrical conductivity, providing the FCMs with the electrical and thermal conductivity and electromagnetic shielding performance. Moreover, an increase in NCGF content and number of NCGFC layers improves the conductive, thermal and electromagnetic shielding properties of the FCMs. The electrical conductivity of the FCM containing 20 wt.% NCGFs reaches 6.68 × 10−6 S/m. The electromagnetic shielding properties of FCM containing both NCGF and NCGFC exceeds 20 dB. Overall, this study provides a new method for constructing fiber weave shielding composites and expands the application field of glass fibers reinforced composites.

The exceptional adhesive properties of polydopamine (PDA) and its ability to perfectly fix various biomolecules and metal ions are conducive for the development of appropriate modifications of implant surfaces. Over the recent years, several combinations of different substrates and surface modification molecules and/or ions have been tested effectively with PDA. In addition, a number of these combinations were proved to have good biocompatibility and specific functions. Therefore, this article summarizes the latest developments in implant surface modification using PDA, in various fields ranging from orthopedics and dentistry to corneal and cardiovascular implants. In addition to osteogenesis, the antibacterial and anti-inflammatory functions of PDA-coated surfaces are also discussed. These studies suggest that PDA, which has strong adhesive properties and good biocompatibility, has potential for use in the surface modification of a variety of functional implants.

Hypoxia can promote the progression and metastasis of ovarian cancer, while the underlying mechanisms are still unclear. Hypoxia culture or CoCl2 induced-oxygen deprivation condition could promote SKOV3 cells to express cyclooxygenase-2 (COX2). Luciferase assay indicates that hypoxia-inducible factor 1α (HIF1α) could bind directly with the promoter region of COX2 to promote the transcription. COX2 over-expressed SKOV3 cells show up-regulated stemness-related markers expression, proinflammatory gene expression, and increased tumor sphere formation. The inflammatory molecules (interleukin-6, C-X-C motif chemokine ligand 12, interleukin-1B, interleukin-10, and C-C motif chemokine ligand 2) and COX2 expression show positive correlations in the Cancer Genome Atlas data. COX2 over-expression could promote SKOV3 cell proliferation in the subcutaneous tumor model and metastasis in the transfer model. In conclusion, hypoxia-induced HIF-1α mediated COX2 expression could promote the proliferation, inflammation, and metastasis of ovarian cancer.

Nowadays, dynamic mixers are widely used in industry. But dynamic mixers have problems like low mixing efficiency and high mixing resistance. Therefore, many scholars are committed to improving the rotor inside the dynamic mixer to improve its working performance. In this paper, a new structure of spiral rotor with chamfer and perforation was designed for promoting the performance of the dynamic mixing equipment. We measured the pH value to find which rotor’s working performance is better. On this basis, a numerical simulation was conducted on the mixing angle of the two-phase fluid to explore the best working environment of the dynamic mixer. Experimental studies proved that the mixing effect of the new structure rotor was better. The simulation results showed that the mixing effect of the two-phase flow was better when it merged at 135°.

The emergence of single-cell RNA sequencing enables simultaneous sequencing of thousands of cells, making the analysis of cell population heterogeneity more efficient. In recent years, single-cell RNA sequencing has been used in the investigation of heterogeneous cell populations, cellular developmental trajectories, stochastic gene transcriptional kinetics, and gene regulatory networks, providing strong support in life science research. However, the application of single-cell RNA sequencing in the field of oral science has not been reviewed comprehensively yet. Therefore, this paper reviews the development and application of single-cell RNA sequencing in oral science, including fields of tissue development, teeth and jaws diseases, maxillofacial tumors, infections, etc., providing reference and prospects for using single-cell RNA sequencing in studying the oral diseases, tissue development, and regeneration.

In order to solve the application restrictions of deterministic-based topology optimization methods arising from the omission of uncertainty factors in practice, and to realize the calculation cost control of reliability-based topology optimization. In consideration of the current reliability-based topology optimization methods of continuum structures mainly based on performance indexes model with a power filter function. An efficient probabilistic reliability-based topology optimization model that regards mass and displacement as an objective function and constraint is established based on the first-order reliability method and a modified economic indexes model with a composite exponential filter function in this study. The topology optimization results obtained by different models are discussed in relation to optimal structure and convergence efficiency. Through numerical examples, it can be seen that the optimal layouts obtained by reliability-based models have an increased amount of material and more support structures, which reveals the necessity of considering uncertainty in lightweight design. In addition, the reliability-based modified model not only can obtain lighter optimal structures compared with traditional economic indexes models in most circumstances, but also has a significant advantage in convergence efficiency, with an average increase of 44.59% and 64.76% compared with the other two reliability-based models. Furthermore, the impact of the reliability index on the results is explored, which verifies the validity of the established model. This study provides a theoretical reference for lightweight or innovative feature-integrated design in engineering applications.

Antibacterial activity is one of the most vital characteristics for Titanium (Ti) dental implants. Coating antibacterial material onto Ti surfaces is an effective approach to enhance their intrinsic antibacterial ability. However, a cost-effective but efficient coating strategy for realizing this objective still remains challenging. In this study, we proposed a novel implant surface modification strategy for coating silver nanoparticles onto the porous Ti surface via a facile electron beam evaporation (EBE) approach. Porous Ti surfaces were firstly prepared by sand-blasting large grit acid-etching (SLA) process. Then, the silver nanoparticles coating thickness on the porous Ti surface was adjusted and optimized by altering the duration of EBE process. Consequently, composite porous Ti surfaces with different silver thicknesses were synthesized. Polished Ti (PT) surface without SLA or EBE process was also prepared as the controlled blank group. The surface characterizations were analyzed by SEM, AFM, and XPS. After that, the antibacterial properties of all groups were tested with bacteria counting method, bacterial viability test, live/dead bacterial staining, and SEM examination. Results show that silver nanoparticles were uniformly distributed on the porous Ti surfaces after the SLA and EBE processes. After being incorporated with silver nanoparticles, the composite surfaces successfully inhibited the growth of Escherichia coli (E. coli) and Staphylococcus aureus (S. aureus). The antibacterial ratio (AR) values of SLA-Ag groups increased with the increasing silver thickness and are significantly higher than those of PT and SLA groups. Therefore, by the SLA and EBE processes, the composite porous Ti surfaces modified with silver nanoparticles coatings demonstrate superior antibacterial property compared with pure Ti surfaces, which is highly promising for enhancing the antibacterial functions of dental implants.

In view of the main contradiction between traditional topology optimization for basic theory research and the actual requirements of comprehensive performance for modern industrial products, a feature-integrated structural optimization design method with an appropriate reliability, a light weight, and excellent mechanical properties for continuum structures was established based on the reliability-based topology optimization of modified minimum weight with a displacement constraint (RBTO-MMWDC) model. Taking hollow slab structures as an example, a graphical user interface based on the optimization code was developed for practical applications. Furthermore, the topological layout of $\beta =3.0$ was selected for geometric model reconstruction and mechanical property evaluations were performed using the finite element method (FEM) and experiments. Compared with those of the traditional structure, the compression resistance and bending resistance properties of the optimized structure are improved by 29% and 75%, respectively, and the first-order natural frequency is increased by 101%. In addition, the results of the experiments are consistent with the simulation in terms of tendency. The bending resistance strength and stiffness of the optimized structure are improved by 61% and 55%, respectively. This study provides a theoretical reference and feasible solution for the feature-integrated design of continuum structures in engineering applications.

Poly(methyl methacrylate) (PMMA)/nano-silica (nano-SiO2) nanocom-posite film with 256 layers containing different amounts of nano-SiO2 was manufactured by a new type of micro-nano multilayer co-extrusion technology. The structure, morphology and mechanical properties of PMMA/nano-SiO2 nanocomposite films were investigated through Fourier transform infrared spectroscopy (FTIR), scanning electron microscope (SEM) and tensile test Besides, molecular dynamics simulation ^J^ was adopted to study the dispersion and content effect on mechanical properties of PMMA/nano-SiO2 nanocomposite film. It is demonstrated that the tensile strength and the elongation at break of the composites improved with increasing nano-SiO2 content from 0 to 5 wt%, which was in good agreement with the Molecular Dynamics simulation. And the nanofiller dispersion in the multilayer nanocomposite film was better than that in the single-layer film with equivalent thickness under the effect of torsion lamination. Overall, the best performance was found for the nanocomposites of PMMA with nano-SiO2 content of 3.5 wt%.

Primary Sjögren's syndrome (pSS) is an autoimmune disease that primarily affects exocrine glands and is characterized by sicca syndrome and systemic manifestation. Mounting evidence indicates that circadian clocks are involved in the onset and progression of autoimmune diseases, including rheumatic arthritis, multiple sclerosis, and systemic lupus erythematosus. However, few studies have reported the expression of clock genes in pSS. There is no ideal therapeutic method for pSS, the management of pSS is mainly palliative, aims to alleviate sicca symptoms. Melatonin is a neuroendocrine hormone mainly secreted by the pineal gland that plays an important role in the maintenance of the circadian rhythm and immunomodulation. Hence, this study aimed to analyse the circadian expression profile of clock genes in pSS, and further evaluate the therapeutic potential of melatonin in pSS. We discovered a distinct clock gene expression profile in the salivary glands of pSS patients and pSS animal model. More importantly, melatonin administration improved the hypofunction of the salivary glands, inhibited inflammatory development, and regulated clock gene expression in animal model of pSS. Our study suggested that the pathogenesis of pSS might correlate with abnormal expression of circadian genes, and that melatonin might be a potential candidate for prevention and treatment of pSS.

Background Excessive inflammatory reactions participate in primary Sjögren’s syndrome (pSS) progression. In addition, biological clock genes have been detected in the salivary glands, which indicates that clock genes regulate the growth and development of the salivary glands as well as the quality and quantity of saliva secretion. Melatonin is an amine hormone secreted by the pineal gland that has many physiological functions, such as regulating immunity and correcting disorder in the biological clock rhythm. The purpose of this study was to clarify the correlation between pSS and the biological clock rhythm and explore the possibility of applying melatonin to treat pSS. Methods Melatonin (10 mg/kg/d or 15 mg/kg/d) or vehicle was administered to NOD/Ltj mice by intraperitoneal injection for 4 weeks. Clock gene expression levels in labial gland biopsy specimens from pSS patients and submandibular gland specimens from mice were measured by Western blotting (WB) and RT-PCR. The salivary flow rate of mice was measured at 12, 14, and 16 weeks. The severity of lymphocyte infiltration in the salivary glands was analysed by haematoxylin and eosin (H&E) staining. Enzyme-linked immunosorbent assay (ELISA) and immunohistochemical staining were used to detect the expression levels of related inflammatory factors in mice. The percentages of Th17, Th2, and Treg cells were analysed by flow cytometry. Results There was a distinct expression profile for clock genes in pSS patients compared with controls. Continuous melatonin administration improved salivary gland function in NOD/Ltj mice, with decreased lymphocyte infiltration in the submandibular glands and reduced related inflammatory factor expression in the serum and salivary glands. Melatonin treatment skewed T cells towards the Treg and Th2 subsets while suppressing Th17 responses. Additionally, melatonin administration regulated clock gene expression in NOD/Ltj mice. Conclusion pSS pathogenesis and progression are correlated with abnormal circadian gene expression. Melatonin improves the hypofunction of the salivary glands and inhibits the inflammatory development of pSS in NOD/Ltj mice. This study provides a theoretical basis and potential approach for the clinical prevention and treatment of pSS.

The human antigen R (HuR) could play an essential role in stabilizing the mRNAs of many tumor-associated genes. Little research is performed to investigate the relevant mechanism mediated by HuR to promote the progress of ovarian cancer. The Cancer Genome Atlas (TCGA) dataset was retrieved to calculate the correlation between HuR and translocase of inner mitochondrial membrane 44 (TIMM44) expression. HuR expression plasmid, TIMM44 expression plasmid, siRNA HuR, and TIMM44 siRNAs were further transfected into A2780 and SKOV3 cells. The 3'UTR of TIMM44 fragment was cloned into the back of Renilla luciferase in the pSicheck2 dual fluorescent reporter to indicate the interaction between HuR and TIMM44. Cell count and MTT assay were performed to assay the proliferation ability of A2780 and SKOV3 cells. High-level HuR expression in 56 ovarian cancer patients recruited in Zibo Central Hospital was positively correlated with metastasis status and poor prognosis revealed by Kaplan-Meier analysis. Both HuR and TIMM44 can promote the proliferation of SKOV3 and A2780 cells. A high correlation of HuR and TIMM44 expression was testified in the TCGA data. Luciferase reporter assay confirmed that HuR could bind to TIMM44 to maintain the mRNA stability. TIMM44 siRNA administration inhibited the proliferation of SKOV3 cells, which could not be rescued. All of these indicate that the main function of HuR on ovarian cancer proliferation is mediated by TIMM44 through mRNA stability regulation, and HuR/TIMM44 complex can be used as a target to inhibit the proliferation of ovarian cancer cells.

A novel surface plasmon resonance (SPR) immunosuppression chip was developed for the reproducible and label-free detection of amantadine (AM) in animal-derived foods. Under the optimal conditions, the proposed SPR immunochip presented a good signal response to AM in the concentration range of 0.05–25.0 ng mL−1, exhibiting high sensitivity (IC50 4.0 ng mL−1) and a low detection limit (IC15 1.3 ng mL−1) and cross-reactivity (rimantadine 16%; others

Background: Endometrial cancer is a common cause of death in gynecological malignancies. Cisplatin is a clinically chemotherapeutic agent. However, drug-resistance is the primary cause of treatment failure. Objective: Emodin is commonly used clinically to increase the sensitivity of chemotherapeutic agents, yet whether Emodin promotes the role of Cisplatin in the treatment of endometrial cancer has not been studied. Method: CCK-8 kit was utilized to determine the growth of two endometrial cancer cell lines, Ishikawa and HEC-IB. The apoptosis level of Ishikawa and HEC-IB cells was detected by Annexin V / propidium iodide double-staining assay. ROS level was detected by DCFH-DA and NADPH oxidase expression. Expressions of drug-resistant genes were examined by real-time PCR and Western blotting. Results: Emodin combined with Cisplatin reduced cell growth and increased the apoptosis of endometrial cancer cells. Co-treatment of Emodin and Cisplatin increased chemosensitivity by inhibiting the expression of drugresistant genes through reducing the ROS levels in endometrial cancer cells. In an endometrial cancer xenograft murine model, the tumor size was reduced and animal survival time was increased by co-treatment of Emodin and Cisplatin. Conclusion: This study demonstrates that Emodin enhances the chemosensitivity of Cisplatin on endometrial cancer by inhibiting ROS-mediated expression of drug-resistance genes.

Electro-responsive materials are of promising applications in microdevices. A dioctyl terephthalate (DOTP) plasticized polyvinyl chloride (PVC) gel actuator driven by unilateral electrodes was proposed, with which the dynamical creeping deformation of the gel around the anode by electro-stimulation was investigated by in situ Raman spectroscopy. The obtained results reveal that the intensity of the band at 1612 cm−1 was linearly correlated to the ratio of DOTP in PVC gel. The plasticizer concentration evolved after an external electro-stimulation was applied, which indicates the migration of plasticizer around the anode. The migration of the plasticizers to the two sides of the anode consequently drives the deformation of the PVC gel. The memory effect of deformation is due to the residuals of the plasticizers out of their original positions after the removal of electro-stimulation. The understanding of the dynamical deformation of PVC gel in response to electro-stimulations may be helpful for the designing of novel electric actuators.

Objective High mobility group box 3 (HMGB3) plays an important role in the development of various cancer. This study aims to explore whether HMGB3 regulates cervical cancer (CC) progression and elucidate the underlying mechanism. Methods HMGB3 expression in clinical patients' tumor samples were determined by real-time quantitative polymerase chain reaction (qRT-PCR) and western blot. HMGB3 overexpression/knockdown were used to investigate its function. Cell apoptosis and cycle were detected by Annexin V/PI staining and flow cytometry. In vivo tumor model was made by subcutaneous injection of HeLa cells transfected with shRNAs targeting HMGB3 (sh-HMGB31) into the flank area of nude mice. Western blot was used to detect the levels of β-catenin, c-Myc, and matrix metalloproteinase-7 (MMP-7) in Hela and CaSki cells transfected with sh-HMGB3 or shRNAs targeting β-catenin. Results Both messenger RNA and protein levels of HMGB3 were upregulated in CC tissues from patients. High expression level of HMGB3 had positive correlation with serosal invasion, lymph metastasis, and tumor sizes in CC patient. Functional experiments showed that HMGB3 could promote CC cell proliferation both in vitro and in vivo. The expression levels of c-Myc and MMP-7 were increased, resulting in regulating cell apoptosis, cell cycle, and activating Wnt/β-catenin pathway. Conclusions Our data indicated that HMGB3 may serve as an oncoprotein. It could be used as a potential prognostic marker and represent a promising therapeutic strategy for CC treatment.

The exact expression profile and potential involvement of miR-625-5p in the tumor biology of cervical carcinoma are still elusive. In this study, we aimed to analyze the expression status and possible involvements of miR-625-5p in both clinical tissue samples and cell culture of cervical carcinoma. The relative expression levels of miR-625-5p and NF-κB transcript were determined by real-time polymerase chain reaction. Cell proliferation was measured using Cell Counting Kit-8. The protein levels of Cyclin D1, CDK4, NF-κB, and GAPDH were examined by Western blotting. The regulatory effects of miR-625-5p on NF-κB and MALAT1 were interrogated by luciferase reporter assay. We demonstrated that miR-625-5p was downregulated and predicted better survival in cervical carcinoma. Ectopic over-expression of miR-625-5p inhibited cell growth via targeting NF-κB. We further identified MALAT1 as the competitive endogenous long non-coding RNA for miR-625-5p, and over-expression of MALAT1 attenuated the inhibitory effect of miR-625-5p on NF-κB signaling in cervical carcinoma. Our study characterized the suppressive expression of miR-625-5p in cervical carcinoma and unraveled the importance of MALAT1/miR-625-5p/NF-κB signaling in this disease.