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Abstract China is one of the largest contributors to global greenhouse gas (GHG) emissions, and the livestock sector is a major source of non-CO2 GHG emissions. Mitigation of GHG emissions from the livestock sector is beneficial to the sustainable development of the livestock sector in China. This study investigated the provincial level of GHG emissions from the livestock sector between 2000 and 2020 in China, to determine the driving factors affecting the provincial-level GHG emissions from the livestock sector, based on the logarithmic mean Divisia index (LMDI) model, which took into account of technological progress, livestock structure, economic factor, and agricultural population. Moreover, a gray model GM (1, 1) was used to predict livestock GHG emissions in each province until 2030 in China. The results showed that the GHG of Chinese livestock sector was decreased from 195.1 million tons (MT) CO2e in 2000 to 157.2 MT CO2e in 2020. Henan, Shandong, and Hebei provinces were the main contributors to the reduction in Chinese livestock GHG emissions, with their livestock GHG emissions reduced by 60.1%, 53.5% and 45.5%, respectively, in 2020 as compared to 2000. The reduction in GHG emissions from the Chinese livestock sector can be attributed to two main factors: technological progress and the shrinking of the agricultural laborers. In contrast, the agricultural economic development model with high input and high emissions showed a negative impact on GHG emission reduction in China’s livestock sector. Furthermore, the different livestock structure in each province led to different GHG reduction effects on the livestock sector. Under the gray model GM (1,1), the GHG emissions of the livestock sector will be reduced by 33.7% in 2030 as compared with 2020 in China, and the efficiency factor will account for 76.6% of the positive effect of GHG reduction in 2030. The eastern coastal region will be the main contributor to the reduction of GHG emissions from the Chinese livestock sector in 2030. Moreover, recommendations (such as upgrading livestock management methods and promoting carbon emission mitigation industries) should be proposed for the environmentally sustainable development of the livestock sector in the future.

Abstract Resistance to immune checkpoint therapy (ICT) presents a growing clinical challenge. The tumor microenvironment (TME) and its components, namely tumor-associated macrophages (TAMs) and cancer-associated fibroblasts (CAFs), play a pivotal role in ICT resistance; however, the underlying mechanisms remain under investigation. In this study, we identify expression of TNF-Stimulated Factor 6 (TSG-6) in ICT-resistant pancreatic tumors, compared to ICT-sensitive melanoma tumors, both in mouse and human. TSG-6 is expressed by CAFs within the TME, where suppressive macrophages expressing Arg1, Mafb, and Mrc1, along with TSG-6 ligand Cd44, predominate. Furthermore, TSG-6 expressing CAFs co-localize with the CD44 expressing macrophages in the TME. TSG-6 inhibition in combination with ICT improves therapy response and survival in pancreatic tumor-bearing mice by reducing macrophages expressing immunosuppressive phenotypes and increasing CD8 T cells. Overall, our findings propose TSG-6 as a therapeutic target to enhance ICT response in non-responsive tumors.

In recent years, cancer, especially primary liver cancer (including hepatocellular carcinoma and intrahepatic cholangiocarcinoma), has posed a serious threat to human health. In the field of liver cancer, exosomes play an important role in liver cancer initiation, metastasis and interaction with the tumor microenvironment. Exosomes are a class of nanoscale extracellular vesicles (EVs)secreted by most cells and rich in bioactive molecules, including RNA, proteins and lipids, that mediate intercellular communication during physiological and pathological processes. This review reviews the multiple roles of exosomes in liver cancer, including the initiation, progression, and metastasis of liver cancer, as well as their effects on angiogenesis, epithelial-mesenchymal transformation (EMT), immune evasion, and drug resistance. Exosomes have great potential as biomarkers for liver cancer diagnosis and prognosis because they carry specific molecular markers that facilitate early detection and evaluation of treatment outcomes. In addition, exosomes, as a new type of drug delivery vector, have unique advantages in the targeted therapy of liver cancer and provide a new strategy for the treatment of liver cancer. The challenges and prospects of exosome-based immunotherapy in the treatment of liver cancer were also discussed. However, challenges such as the standardization of isolation techniques and the scalability of therapeutic applications remain significant hurdles.

The Gross Domestic Product (GDP) per capita is one of the most widely used socioeconomic indicators, serving as an integral component for climate change impact analysis. However, a national scale assessment may induce considerable bias because it conceals any internal variations within a country. The lack of a long-term sub-national scale GDP data is a substantive hinderance. Leveraging the close relationship between nighttime lights and GDP, we address this gap by developing a novel methodological framework in two steps. First, under the modeling philosophy of spatial statistics, we developed a novel approach based on deep and machine learning techniques to establish a complex mapping between two inconsistent nighttime lights (NTL) datasets: the Defense Meteorological Satellite Program’s Operational Linescan System (DMSP) and the National Polar-Orbiting Partnership’s Visible Infrared Imaging Radiometer Suite (VIIRS). The models achieve accuracies ranging from 0.945 to 0.980 (correlation coefficients). By taking the estimations ensemble of the two techniques, the time series of DMSP data was extended to 2021. Next, a novel modeling strategy based on multi-layer perceptron was developed to derive the non-linear relationship between NTL and GDP per capita at sub-national scale to alleviate scale effects at this granularity, while explicitly capturing regional heterogeneity effect. The trained models achieve average accuracies of 0.967, 0.959, and 0.959 on the training, validation, and test sets, respectively. We evaluate the developed dataset at the global, national, and sub-national scales from various perspective, and the results offer solid evidence on the reliability of the estimated economic data. By linking to historical global climate change data, we quantify global economic losses attributed to extreme heat to demonstrate how the estimated GDP data can be useful in the climate change impact analysis.

Tender bamboo shoots undergo rapid senescence that influences their quality and commercial value after harvest. In this study, the tender sweet bamboo shoots (‘Wensun’) were packed by a passive modified atmosphere packaging (PMAP) to inhibit the senescence process, taking polyethylene package as control. The increase in CO2 and the decrease in O2 gas concentrations in the headspace atmosphere of the packages were remarkably modified by PMAP treatments. The modified gas atmosphere packaging inhibited the changes in firmness, as well as the content of cellulose, total pectin, and lignin in the cell walls of bamboo shoots. The enzymatic activities of cellulase, pectinase, and polygalacturonase that act on cell wall polysaccharides, and phenylalanine ammonia lyase, cinnamyl alcohol dehydrogenase, peroxidase, and laccase regulating the lignin biosynthesis were modified by PMAP treatment different from control during storage. The expression levels of the lignin biosynthesis genes PePAL3/4, PeCAD, Pe4CL5, PeC4H, PeCCOAOMT, PeCOMT, cellulose synthase PeCESA1, and related transcription factors PeSND2, PeKNAT7, PeMYB20, PeMYB63, and PeMYB85 were clearly regulated. These results suggest that PMAP efficiently retards the changes in lignin and cell wall polysaccharides, thus delaying the senescence of tender sweet bamboo shoots during storage.

【Objective】Fresh bamboo shoots are bamboo shoot products that have not been blanched or pickled, with a crispy taste and refreshing flavor. However, fresh bamboo shoots breathe vigorously, which are prone to dehydration and wilting, making them difficult to keep fresh, thus affecting their sales. Spontaneous modified atmosphere packaging reduces the respiratory intensity of packaged fruits and vegetables by regulating the gas composition of the internal environment. Studying the effects of different modified atmosphere packaging treatments on the postharvest storage quality and lignification of fresh bamboo shoots could provide references for the selection of fresh-keeping packaging for fresh bamboo shoots.【Method】The peeled fresh bamboo shoots were packed in the spontaneous modified atmosphere preservation bag (thickness 20μm, O2transmission rate 11 643 cm3/m2·d·atm, water vapor transmission rate 62.586 g/m2·d), and the vacuum bag made of nylon was used as control. The packaged fresh bamboo shoots were stored in the cold storage to study the quality change and the difference of lignification degree during storage.【Result】Compared with traditional vacuum preservation techniques, spontaneous modified atmosphere packaging could significantly reduce the respiratory intensity of fresh bamboo shoots during storage by creating a low oxygen and low carbon dioxide(O2 < 5%, CO2 < 5%) environment inside the bag through the respiration of the bamboo shoots themselves and the gas permeability of the membrane material. The peak intensity was only 46.93% of that of vacuum treatment. After 21 days of storage, modified atmosphere packaging significantly inhibited the browning and membrane lipid peroxidation of bamboo shoots. The brightness of the core of packaged bamboo shoots decreased by only 6.17% compared with that upon harvest, while that of vacuum packaging decreased by 17.74%. The content of malondialdehyde in vacuum packaged bamboo shoots was 2.93 times that in modified atmosphere packaging. In terms of lignification, the lignification rates of bamboo shoots in two types of packaging began to increase after 14 days of storage. The activities of key enzymes for lignification of bamboo shoots (phenylalanine ammonia lyase, peroxidase, cinnamon dehydrogenase) peaked in vacuum packaging, and lignification intensified; however, there was no peak in the related enzyme activity of bamboo shoots in the modified atmosphere packaging, and the enzyme activity increased slowly, delaying the lignification of bamboo shoot and maintaining the crisp taste of the bamboo shoots. After 21 days of storage, the lignin content of packaged bamboo shoots decreased by 15.01% compared to vacuum packaged bamboo shoots, effectively extending the freshness period of fresh bamboo shoots.【Conclusion】The low oxygen and low carbon dioxide gas environment in the spontaneous modified atmosphere packaging bag is conducive to the storage of fresh bamboo shoots, and the quality and nutrient content are significantly better than those of vacuum packaging treatment. It has a good market prospect for fresh bamboo shoots.

During the operation of a shaped hole seed-metering device, poor seed-filling quality and inconsistent seed-casting points lead to poor seed spacing uniformity, especially in a one-chamber double-row seed-metering device. To solve this problem, a soybean double-row seed-metering device with double-beveled seed guide groove was designed to ensure a high single-seed rate and seed-casting point consistency. Through the theoretical analysis of the working process of the seed-metering device, dynamic and kinematic models of the seeds were established, and the main structural parameters of the seed discharge ring, triage convex ridge, shaped hole, and seed guide groove were determined. The main factors affecting the seeding performance were obtained as the following: the inclination angle of the triage convex ridge, the radius of the shaped hole, and the depth of the seed guide groove. A single-factor test was carried out by discrete element simulation to obtain the inclination angle of the triage convex ridge α3 = 29°, the radius of the shaped hole r1 = 4.16–4.5 mm, and the depth of the seed guide groove l1 = 0.49–1.89 mm. A two-factor, five-level, second-order, orthogonal rotation combination test was conducted to further optimize the structural parameters of the seed-metering device. The two test factors were the radius of the shaped hole and the depth of the seed guide groove, and the evaluation indices were the qualified rate, replay rate, and missed seeding rate. The results showed that the optimal combinations of the structural parameters were the radius of the shaped hole r1 = 4.33 mm and the depth of the seed guide groove l1 = 1.20 mm. Subsequent bench testing demonstrated that the seed discharge’s qualified rate was above 94% at operating speeds of 6–10 km/h, and the seeding performance was stable. The final results of the soil trench test showed that the seed-metering device exhibited a qualified rate of 93.31%, replay rate of 2.04%, and missed seeding rate of 4.65% at an operating speed of 8 km/h. This research outcome may serve as a valuable reference and source of inspiration for the innovative design of precision seed-metering devices.

Dynamic disasters seriously threaten the safety and effectiveness in deep mining. The mechanism of stick-slip instability occurrence on the deep coal-rock structure was studied in this paper through analyzing the occurrence condition of stick-slip in the weak surface and discussing the friction constitutive of the coal-rock structure. The result indicated that the grinding slip and the soft interlayer formation occurred in weak plane in the coal-rock structure in the process of diagenesis and strata movement, which is more likely to satisfy the stick-slip condition and then trigger dynamic disaster. Based on the mechanism study of coal-rock structure failure caused by stick-slip, it is proposed that the horizontal compression pulse caused by local stick-slip in coal seam could lead the layer-crack and fly out with the broken fragments. At the same time, it is pointed out that the scattering effect of the plane compression pulse in the propagation process induces the serial sliding instability of the coal-rock structure in many places, forming a large range of dynamic disasters. By means of numerical simulation of coal bump accident in a roadway in Yima Coal Mines, the dynamic process of sliding-slip instability of coal and rock mass is inverted, and the mechanism of sliding-slip instability of coal and rock structure is verified.

ABSTRACT During high-speed seeding operations, collisions between seeds and seed guide tubes are very significant, resulting in poor seed spacing uniformity. To solve this problem, a high-speed seed guide device with conical spiral airflow was designed, which can use high-speed positive pressure airflow to move seeds smoothly. To improve the uniformity of the airflow field in the device, a single-factor test was carried out to determine the reasonable range of each factor. Then, the four-factor and five-level central composite test was carried out. The four main factors were screw pitch, cone angle, width of gap, and angle of air-tube, and the evaluation index was the mean variance of airflow velocity. The test results showed that the optimal parameters of the high-speed seed guide device with conical spiral airflow were a screw pitch of 24.98 mm, cone angle of 7.47/55, width of gap of 5.31 mm, and angle of air-tube of 25.09º. The verification test showed that the relative error between the verification test value and the predicted value was less than 5%, indicating that the model had high reliability. This research can provide references for the research of high-speed seed guide technology and the innovative design of seed guide devices.

Fresh puffer fish (Takifugu obscurus) are susceptible to microbial contamination and have a very short shelf-life of chilled storage. Hence, this study aimed to evaluate the effects of plasma-activated lactic acid (PALA) on microbiota composition and quality attributes of puffer fish fillets during chilled storage. The results showed that PALA treatment effectively reduced the growth of bacteria and attenuated changes in physicochemical indicators (total volatile basic nitrogen, pH value, K value, and biogenic amines) of puffer fish fillets. Additionally, insignificant changes were observed in lipid oxidation during the first 8 days (p > 0.05). Illumina-MiSeq high-throughput sequencing revealed that PALA effectively inhibited the growth of Pseudomonas in puffer fish fillets and maintained the diverse characteristics of the microbial community. In combination with sensory analysis, PALA extended the shelf life of puffer fish fillets for 4 days, suggesting that PALA could be considered a potential fish fillet preservation method.

Abstract Background The social disparities in obesity may originate in early life or in adulthood, and the associations of socioeconomic position (SEP) with obesity could alter over time. It is unclear how lifetime-specific and life-course SEP influence adult obesity development in China. Methods Based on the China Health and Nutrition Survey (CHNS), three SEP-related indicators, including the father’s occupational position and the participant’s education and occupational position, were obtained. The life-course socioeconomic changes and a cumulative SEP score were established to represent the life-course SEP of the participants in the study. The growth mixture modeling was used to identify BMI trajectories in adulthood. Multinomial logistic regression was adopted to assess the associations between SEP and adult BMI trajectories. Results A total of 3,138 participants were included in the study. A positive correlation was found between the paternal occupational position, the participants’ occupational position, education, and obesity in males, whereas an inverse correlation was observed among females. Males who experienced social upward mobility or remained stable high SEP during the follow-up had 2.31 and 2.52-fold risks of progressive obesity compared to those with a stable-low SEP. Among females, stable high SEP in both childhood and adulthood was associated with lower risks of progressive obesity (OR = 0.63, 95% CI: 0.43–0.94). Higher risks of obesity were associated with the life-course cumulative SEP score among males, while the opposite relationship was observed among females. Conclusions The associations between life-course SEP and BMI development trajectories differed significantly by gender. Special emphasis should be placed on males experiencing upward and stable high socioeconomic change.

Objective: To reveal the microbial community and its function succession principles of puffer fish (Takifugu obscurus), and to identify the dominant microbial genera on puffer fish during refrigerated storage. Methods: High-throughput sequencing was employed to investigate the microbial composition of puffer fish during refrigeration (4 ℃, 8 d), the community function was predicted by Tax4fun2, and the total volatile basic-nitrogen (TVB-N), pH and organoleptic values of puffer fish were measured as well. Results: The high-throughput sequencing demonstrated that during refrigeration the bacterial community of puffer fish succeed significantly, which were mainly composed of genera Acinetobacter, Lactobacillus, Aeromonas and Pseudomonas at the initial stage, while at the last stage composed almost of genera Pseudomonas (>91.7%), which was considered as the dominant genera on puffer fish. The α-diversity index of the community was higher at initial stage, while decreased during the storage. The microbial community function prediction results showed that the relative abundance of the amino-acid metabolism pathway and the cellular communication pathway increased at the last stage of the storage. As for the physicochemical properties, TVB-N, pH and total microbial counts increased while sensory scores decreased during the storage. Conclusion: The microbial community and its functional profile changed significantly during refrigeration, Pseudomonas was the dominant genera of puffer fish, and the above conclusion would provide theoretic guidelines for developing novel preserving technology for puffer fish.

Abstract Green manufacture of steroid precursors from diosgenin by microbial replacing multistep chemical synthesis has been elusive. It is currently limited by the lack of strain and degradation mechanisms. Here, we demonstrated the feasibility of this process using a novel strain Mycolicibacterium sp. HK‐90 with efficiency in diosgenin degradation. Diosgenin degradation by strain HK‐90 involves the selective removal of 5,6‐spiroketal structure, followed by the oxygenolytic cleavage of steroid nuclei. Bioinformatic analyses revealed the presence of two complete steroid catabolic gene clusters, SCG‐1 and SCG‐2, in the genome of strain HK‐90. SCG‐1 cluster was found to be involved in classic phytosterols or cholesterol catabolic pathway through the deletion of key kstD1 gene, which promoted the mutant m‐∆kstD1 converting phytosterols to intermediate 9α‐hydroxyandrostenedione (9‐OHAD). Most impressively, global transcriptomics and characterization of key genes suggested SCG‐2 as a potential gene cluster encoding diosgenin degradation. The gene inactivation of kstD2 in SCG‐2 resulted in the conversion of diosgenin to 9‐OHAD and 9,16‐dihydroxy‐pregn‐4‐ene‐3,20‐dione (9,16‐(OH)2‐PG) in mutant m‐ΔkstD2. Moreover, the engineered strain mHust‐ΔkstD1,2,3 with a triple deletion of kstDs was constructed, which can stably accumulate 9‐OHAD by metabolizing phytosterols, and accumulate 9‐OHAD and 9,16‐(OH)2‐PG from diosgenin. Diosgenin catabolism in strain mHust‐ΔkstD1,2,3 was revealed as a progression through diosgenone, 9,16‐(OH)2‐PG, and 9‐OHAD to 9α‐hydroxytestosterone (9‐OHTS). So far, this work is the first report on genetically engineered strain metabolizing diosgenin to produce 21‐carbon and 19‐carbon steroids. This study presents a promising biosynthetic platform for the green production of steroid precursors, and provide insights into the complex biochemical mechanism of diosgenin catabolism.

Phthalides are a class of unique compounds such as ligustilide, butylphthalide and butyldenephthalide, which have shown to possess multiple bioactivities in new drug research and development. Phthalides are naturally distributed in different plants that have been utilized as herbal treatments for various ailments with a long history in Asia, Europe and North America. Their extensive biological activity has led to a dramatic increase in the study of phthalide compounds in recent years. This review summarizes the latest research progress of plant-derived phthalides, and a total of 133 phthalide compounds are described based on the characteristics of chemical structures. Pharmacological properties of plant-derived phthalides are associated with hemorheological improvement, vascular function modulation and central nervous system protection. Potential treatments for a variety of diseases mainly including cardio-cerebrovascular disorders and neurological complications such as Alzheimer's disease are also concluded. In addition, key metabolic pathways have been clearly elucidated. Further investigations on the molecular mechanisms involved in biological activity are recommended for offering new insights into profound comprehension of phthalide applications.

AbstractLife skills (LS) training programs help individuals develop their mental health and stress-related attitudes. This study aims to build a versatile physical education program (PEP) to promote LS and examine its effectiveness. The subjects were first-year Japanese university students. We conducted the LS survey in April (pre-semester) and August (post-semester) 2019. To analyze the difference in LS subscales scores depending on the PEP at the pre-semester/post-semester, we conducted two-way ANOVA repeated measures with the Semester-Sports item and examined simple main effects. As a result, there were significant interactions on the subscales “leadership (F (2,285) = 8.20, P

Abstract The combination of PD-1 blockade with neoadjuvant chemotherapy (NAC) has achieved unprecedented clinical success in non-small cell lung cancer (NSCLC) compared to NAC alone, but the underlying mechanisms by which PD-1 blockade augments the effects of chemotherapy remain incompletely elucidated. Single-cell RNA sequencing was performed on CD45+ immune cells isolated from surgically resected fresh tumors of seven NSCLC patients receiving NAC or neoadjuvant pembrolizumab and chemotherapy (NAPC). Multiplex fluorescent immunohistochemistry was performed on FFPE tissues before and after NAC or NAPC from 65 resectable NSCLC patients, and results were validated with GEO dataset. NAC resulted in an increase only of CD20+ B cells, whereas NAPC increased the infiltration of CD20+ B cells, CD4+ T cells, CD4+CD127+ T cells, CD8+ T cells, CD8+CD127+ and CD8+KLRG1+ T cells. Synergistic increase in B and T cells promotes favorable therapeutic response after NAPC. Spatial distribution analysis discovered that CD8+ T cells and their CD127+ and KLRG1+ subsets were in closer proximity to CD4+ T/CD20+ B cells in NAPC versus NAC. GEO dataset validated that B-cell, CD4, memory, and effector CD8 signatures correlated with therapeutic responses and clinical outcomes. The addition of PD-1 blockade to NAC promoted anti-tumor immunity through T and B cells recruitment in the tumor microenvironment and induced tumor-infiltrating CD8+ T cells skewed toward CD127+ and KLRG1+ phenotypes, which may be assisted by CD4+ T cells and B cells. Our comprehensive study identified key immune cell subsets exerting anti-tumor responses during PD-1 blockade therapy and that may be therapeutically targeted to improve upon existing immunotherapies for NSCLC.

The thermos-structural materials with high strength and high temperature water-oxygen corrosion resistance are urgently needed in aero-space fields. SiC whisker reinforced SiC matrix (SiCw/SiC) composites are the ideal candidate materials for their excellent mechanical properties and thermostability. However, the poor resistance to water-oxygen corrosion ability of SiC matrix limits their further application. In this work, a novel SiCw/SiC–Si–Y composites were first prepared by infiltrating silicon-yttrium (Si–Y) alloy into SiCw/C preform via reactive melt infiltration method. The as-obtained composites not only show the excellent mechanical properties (σF = 363 MPa, KⅠC = 9.14 MPa m1/2), and more importantly after water-oxygen corrosion of 20 h at such temperature as high as 1500 °C, the strength and toughness retention of the composites can reach 106.1% and 103.6%, respectively. The SiCw/SiC–Si–Y composites developed in this paper have a great potential as a new high-performance thermos-structural material for aero-space applications.

Abstract Using goof as water storage space plays a vital role in the ecological environment and economic development of arid mining areas, while the rock strength and the stability of coal pillars in underground water reservoirs are closely related to creep process. In this work, triaxial creep-seepage tests were conducted for coal samples to develop new insights into the creep behavior and permeability evolution. The results showed that the creep deformation and permeability evolution of coal samples exhibit three stages, namely, the compaction hardening stage before the stress threshold, volumetric compaction stage, and volumetric dilatancy stage. The coal permeability decreases first and then increases with the creep strain and it is well correlated with the variation of volumetric strain.

Background: Abnormal levels of monosaccharides in blood have been linked to tumorigenesis. In this study, a novel high-performance liquid chromatography (HPLC) method was established for the simultaneous determination of free mannose and glucose in the serum.Methods: The serum was directly derivatized by 1-phenyl-3-methyl-5-pyrazolone under alkaline conditions using L-rhamnose as an internal standard. The chromatographic separation was then performed on a Poroshell EC-C18 chromatographic column (4.6 × 100 mm, particle size 2.7 μm, Agilent) with gradient elution using NH4Ac-HAc and acetonitrile as the mobile phases. The method was thereafter validated according to international guidelines. The serum samples obtained from 200 healthy individuals and 200 ovarian cancer (OC) patients were analyzed for free mannose and glucose.Results: The method was found to be reproducible for quantification within 20 min and included online sample purification. The method displayed excellent linearity in the concentration range (for mannose: 0.5–500 μg/mL; glucose: 0.5–1500 μg/mL). The precision, recovery, and stability met the FDA bioanalytical method validation acceptance criteria. Overall, the measurement of glucose content by HPLC correlated well with the different enzymatic methods. Ovarian cancer mannose levels in the serum were significantly higher in the advanced stage (61.22 μmol/L, p < 0.0001) than those in healthy volunteers and early-stage patients (44.51 μmol/L versus 50.09 μmol/L, p < 0.0001). The AUC for the ratio of serum free glucose to mannose (G/M) was 0.98 (p < 0.0001), with a sensitivity of 91.46% and a specificity of 98.50%, which served as a biomarker for OC diagnosis.Conclusion: We report a simple, repeatable, and attractive analytical method by HPLC, which can be used for quantitative estimation of free mannose and glucose simultaneously in human serum. Our results indicate that the serum level of mannose could be used as a potential biomarker of ovarian cancer.

The application of hydrogen-rich water (HRW) can extend the shelf life of fruits and vegetables. This study aims to investigate the effects of HRW on the quality of fresh-cut Gastrodia elata during low-temperature (4°C) storage. The results indicate that HRW treatment inhibited the decrease in weight loss and the increase in respiration rate, and also slowed down the decrease in total acidity and total soluble solids. HRW treatment could reduce the generation of superoxide anions and hydrogen peroxide, enhance the activity and expression of antioxidant-related enzymes such as superoxide dismutase, catalase, ascorbate peroxidase, glutathione reductase, monodehydroascorbate reductase, and dehydroascorbate reductase, and increase the content of non-enzymatic antioxidant substances, ascorbic acid and glutathione, while decreasing the activity and expression of peroxidase and polyphenol oxidase. Furthermore, HRW treatment not only inhibited the reduction in ADP and ATP content and energy charge but also attenuated the increase in AMP content. It also slowed down the decrease in the activities of H+-ATPase, succinate dehydrogenase, Ca2+-ATPase, and cytochrome C oxidase. In conclusion, these results demonstrate that HRW treatment enhances the energy levels and effectively mitigates oxidative damage, thereby preserving postharvest quality of fresh-cut G. elata.

Background: Neoadjuvant immunotherapy, the focus of current research and treatment modality for long-term survival, has become one of the main options in supporting primary treatment interventions in early NSCLC. Methods: This was a retrospective analysis of patients with locally resectable NSCLC who received the neoadjuvant drug pembrolizumab combined with chemotherapy and underwent surgical resection. Pathological responses, PFS and OS in the whole sample and subgroups were analyzed. Results: Of the 61 patients included in this retrospective analysis, 31 (50.82%) achieved a pCR, and 38 (62.30%) obtained an MPR. Patients with a pCR had significantly higher OS than the non-pCR group (HR = 0.093, P = 0.0227); patients with an MPR also had significantly elevated OS compared with the non-MPR group (HR = 0.05357, P = 0.0169). Patients with lymph node metastasis after surgery had significantly reduced OS (HR = 0.01607, p = 0.0004) and PFS (HR = 0.08757, p = 0.0004) than those without lymph node metastasis. There was no significant difference in OS and PFS between squamous cell carcinomas (SCC) group and adenocarcinomas (AD) group. No significant differences in OS and PFS were found between patients administered 2 and 3 cycles of neoadjuvant therapy before surgery, between those administered ≤5 and > 5 cycles of adjuvant therapy post-surgery, and between patients with TPS 0.05). Conclusion: Neoadjuvant immunochemotherapy with pembrolizumab plus chemotherapy in non-small cell lung cancer is safe and tolerable. Both pCR and MPR were closely associated with OS and PFS, reflecting a good response of tumor tissues to drug therapy. Lymph node metastasis after surgery was a poor prognostic factor, reducing OS and PFS.

Quercetin (QR) is a natural flavonol compound widely distributed in the plant kingdom with extensive pharmacological effects. To find the potential clinical indications of QR, 156 differentially expressed genes (DEGs) regulated by QR were obtained from the Gene Expression Omnibus database, and new potential pharmacological effects and clinical indications of QR were repurposed by integrating compounds with similar gene perturbation signatures and associated-disease signatures to QR based on the Connectivity Map and Coexpedia platforms. The results suggested QR has mainly potential therapeutic effects on multiple sclerosis (MS), osteoarthritis, type 2 diabetes mellitus, and acute leukemia. Then, MS was selected for subsequent animal experiments as a representative potential indication, and it found that QR significantly delays the onset time of classical MS model animal mice and ameliorates the inflammatory infiltration and demyelination in the central nervous system. Combined with network pharmacology technology, the therapeutic mechanism of QR on MS was further demonstrated to be related to the inhibition of the expression of inflammatory cytokines (TNF-α, IL-6, IL-1β, IFN-γ, IL-17A, and IL-2) related to TNF-α/TNFR1 signaling pathway. In conclusion, this study expanded the clinical indications of QR and preliminarily confirmed the therapeutic effect and potential mechanism of QR on MS.

Breast cancer stands as the most prevalent malignancy globally. Despite the array of treatment options, its mortality rate continues to rise annually. Thus, reevaluation of factors directly linked to breast cancer emergence is imperative, alongside the development of more effective preventive measures. Estrogen levels, profoundly tied to reproduction, play a pivotal role in breast cancer development. Speculation centers on the potential of breastfeeding to mitigate cancer risk in women. However, the precise mechanism remains elusive. Breastfeeding is a modifiable reproductive factor extensively studied. Studies highlight a direct connection between lack of breastfeeding and breast cancer emergence, potentially linked to DNA methyltransferase expression alteration, aberrant methylation levels, pregnancy-associated plasma protein-A, cellular microenvironment, and oncogenes. This study reviews recent mechanisms underlying breastfeeding’s role in reducing breast cancer incidence.

Abstract Objectives A classification system for endocervical adenocarcinoma (ECA) based on high‐risk human papillomavirus (HPV) status has been established; however, the immunohistochemical markers distinguishing HPV‐independent and HPV‐associated ECAs have not been fully described. Here, we aimed to characterize ECA immunopathological features. Methods We evaluated the immunohistochemical profile of CLDN18, CDX2, PAX8, p16, p53, and CEA in 60 ECAs comprising 10 HPV‐independent ECAs and 50 HPV‐associated ECAs. Both the membranous and nuclear expression levels of CLDN18 were analyzed. Results Membranous CLDN18 (CLDN18 [M]) was found to be expressed in the mucinous epithelium of all HPV‐independent ECAs, including eight gastric‐type ECAs (G‐ECAs), one endometrioid ECA, and one clear cell ECA, but no nuclear CLDN18 (CLDN18 [N]) expression was detected in HPV‐independent ECAs. Among HPV‐associated ECAs, CLDN18 (M) expression levels in intestinal‐type (I‐ECAs) and usual‐type ECAs (U‐ECAs) were significantly different from those in invasive stratified mucin‐producing (iSMILE) carcinomas (p = 0.036). Positive CLDN18 (M) staining was present in 55.6% (5/9) of intestinal‐type and 39.4% (13/33) of usual‐type ECAs and was not present in iSMILE ECAs. Silva pattern C cancers expressed higher levels of CLDN18 (M) than Silva pattern A and B cancers (p = 0.004), whereas the CLDN18 (N) expression levels in cancers showing Silva pattern A were significantly higher than those in cancers exhibiting Silva patterns B and C (p

Abstract Chromosomes occupy discrete spaces in the interphase cell nucleus, called chromosome territory. The structural and functional relevance of chromosome territory remains elusive. We fused chromosome 15 and 17 in mouse haploid embryonic stem cells (haESCs), resulting in distinct changes of territories in the cognate chromosomes, but with little effect on gene expression, pluripotency and gamete functions of haESCs. The karyotype-engineered haESCs were successfully implemented in generating heterozygous (2n = 39) and homozygous (2n = 38) mouse models. Mice containing the fusion chromosome are fertile, and their representative tissues and organs display no phenotypic abnormalities, suggesting unscathed development. These results indicate that the mammalian chromosome architectures are highly resilient, and reorganization of chromosome territories can be readily tolerated during cell differentiation and mouse development.

Cancer metastasis is a major cause of cancer-related mortality. Strategies to reduce metastases are needed especially in lung cancer, the most common cause of cancer mortality. We previously reported increased ubiquitin-specific peptidase 18 (USP18) expression in lung and other cancers. Engineered reduction of USP18 expression repressed lung cancer growth and promoted apoptosis. This deubiquitinase (DUB) stabilized targeted proteins by removing the complex interferon-stimulated gene 15 (ISG15). This study explores if the loss of USP18 reduced lung cancer metastasis. USP18 knock-down in lung cancer cells was independently achieved using small hairpin RNAs (shRNAs) and small interfering RNAs (siRNAs). USP18 knock-down reduced lung cancer growth, wound-healing, migration, and invasion versus controls (P

Background and objective At present, robotic surgery is widely used in thoracic surgery, which has higher maneuverability, precision, and stability, especially for small space complex operations and reconstructive surgery. The advantages of robotic lung segment resection under full orifice artificial pneumothorax are obvious. Methods Based on a large number of clinical practices, we established a set of surgical methods for 4-arm robotic lung segment resection under a port-only artificial pneumothorax. 98 cases of robotic lung segment resection were performed with this method from January 2019 to August 2022. The clinical experience was summarized. Results Robotic lung segment resection under port-only artificial pneumothorax has obvious advantages in the anatomy of lung segment vessels and bronchi. It is characterized by less bleeding, shorter operation time, adequate exposure, and flexible operation. Conclusion This surgical model we propose optimizes the operation mode and technique of lung segment resection, makes each step procedural, reduces collateral damage, and is easy to learn and master, which is believed to cure more lung cancer patients with less trauma.

The development of renewable clean energy wind power is very rapid. However, the strong randomness and weak dispatchability of wind power bring significant challenges to wind power operation and maintenance, such as grid connection, power dispatch, and unit combination. At this stage, the combined model based on signal decomposition-prediction technology plays an essential role in ultra-short-term deterministic wind power prediction, promoting the development of the wind power industry. With the development of artificial intelligence technology, this paper discusses and summarizes the latest research progress on the critical technologies of the combined signal decomposition-prediction technology model to improve the prediction accuracy for the problem of high demand for ultra-short-term power prediction accuracy. Data decomposition from two perspectives of decomposition techniques reduces input data complexity and feature extraction by eliminating redundant information. The prediction techniques are from two perspectives: prediction methods to demonstrate specific laws and parameter search to determine the optimal parameters. Three perspectives evaluated prediction performance: vertical error evaluation, horizontal error evaluation, and prediction error evaluation by analyzing the above standard methods in detail, including the principle, idea, application examples, advantages and disadvantages, key points, and application notes of each technique. And the prediction performance of the current combined models is compared extensively. Finally, the future research trends of wind power ultra-short term are discussed and foreseen to provide a reference for the development of ultra-short-term power prediction technology, prediction performance improvement, and safe and stable grid operation.

Background: Migraine is a common neurovascular disorder with typical throbbing and unilateral headaches, causing a considerable healthcare burden on the global economy. This research aims to prepare chitosan-alginate (CS-AL) nanoparticles (NPs) containing Foshousan oil (FSSO) and investigate its potential therapeutic effects on the treatment of migraine.Methods: FSSO-loaded CS-AL NPs were prepared by using the single emulsion solvent evaporation method. Lipopolysaccharide (LPS)-stimulated BV-2 cells and nitroglycerin (NTG)-induced migraine mice were further used to explore anti-migraine activities and potential mechanisms of this botanical drug.Results: FSSO-loaded CS-AL NPs (212.1 ± 5.2 nm, 45.1 ± 6.2 mV) had a well-defined spherical shape with prolonged drug release and good storage within 4 weeks. FSSO and FSSO-loaded CS-AL NPs (5, 10, and 15 μg/mL) showed anti-inflammatory activities in LPS-treated BV-2 cells via reducing the levels of pro-inflammatory cytokines such as tumor necrosis factor-α (TNF-α), interleukin-1β (IL-1β), interleukin-6 (IL-6), and nitric oxide (NO), but elevating interleukin-10 (IL-10) expressions. Moreover, FSSO-loaded CS-AL NPs (52 and 104 mg/kg) raised pain thresholds against the hot stimulus and decreased acetic acid-induced writhing frequency and foot-licking duration in NTG-induced migraine mice. Compared with the model group, calcitonin gene-related peptide (CGRP) and NO levels were downregulated, but 5-hydroxytryptamine (5-HT) and endothelin (ET) levels were upregulated along with rebalanced ET/NO ratio, and vasomotor dysfunction was alleviated by promoting cerebral blood flow (CBF) in the FSSO-loaded CS-AL NPs (104 mg/kg) group.Conclusion: FSSO-loaded CS-AL NPs could attenuate migraine via inhibiting neuroinflammation in LPS-stimulated BV-2 cells and regulating vasoactive substances in NTG-induced migraine mice. These findings suggest that the FSS formula may be exploited as new phytotherapy for treating migraine.

Water reducing agent as an important admixture. It is used to decrease the initial water content in cement paste, improving its fluidity. Polycarboxylic acid water reducing agent (PCE) is a high-performance type with advantages such as low dosage, high water reduction rate, environmental friendliness, and a simple synthesis process. In this study, the third monomer (TPEG-SAA-SHES) was synthesized by modifying isopentenol polyoxyethylene ether (TPEG) with sodium hydroxyethyl sulfonate (SHES). Subsequently, a modified ether polycarboxylic acid-based water reducing agent (PCE-S) was synthesized with TPEG and acrylic acid (AA). The experimental conditions for the synthesis of PCE-S were optimized through orthogonal tests. The synthesized PCE-S showed a certain degree of improvement in the initial net slurry flow compared to PCE, with a maximum enhancement of 19.64%. The adsorption increased by 18.25% when the TSS dosage was 5%. PCE-S enhances the hydration products of cement, increasing the compactness of the structure. The product is environmentally friendly, safe, and durable, effectively reducing the dosage of additives and helping to cut costs. It lays the foundation for the next industrialized mass production.

Neighbor distinguishing colorings of graphs represent powerful tools for solving the channel assignment problem in wireless communication networks. They consist of two forms of coloring: neighbor distinguishing edge coloring, and neighbor distinguishing total coloring. The neighbor distinguishing edge (total) coloring of a graph G is an edge (total) coloring with the requirement that each pair of adjacent vertices contains different color sets. The neighbor distinguishing edge (total) chromatic number of G is the smallest integer k in cases where a neighbor distinguishing edge (total) coloring exists through the use of k colors in G. The maximum average degree of G is the maximum of the average degree of its non-empty subgraphs. In this paper, we characterize the neighbor distinguishing edge (total) chromatic numbers of graphs with a maximum average degree less than four by means of the discharging method.

The widespread use of pharmaceuticals and personal care products (PPCPs) in many fields has brought convenience to human lives but has also caused unavoidable environmental pollution issues. In particular, the resistance gene problem resulting from accumulating antibiotics that cannot be fully absorbed by biological individuals has been a concern; thus, it is urgent to find efficient technologies to boost the degradation efficiency of antibiotics in the environment. Here, an ε-MnO2 catalyst was prepared by a novel droplet-interface-drying method and utilized as a Fenton-like catalyst for efficiently degrading ciprofloxacin (CIP). The ε-MnO2 shell was formed preferentially at the gas–liquid interface and then continued to decompose into ε-MnO2 with abundant O vacancies in the air-insulated microcavity. The XPS result confirms that this particular preparation method can regulate the content of O vacancies in the material. Compared with ε-MnO2 samples obtained by the direct drying method (ε-MnO2-B), the catalytic performance of ε-MnO2 prepared by the droplet-interface-drying method (ε-MnO2-P) is significantly improved. By activating peroxymonosulfate (PMS) with the ε-MnO2-P catalyst, the CIP degradation efficiency can reach 84.1%. The detection and analysis of reactive oxygen species (ROS) in the ε-MnO2-P/PMS oxidation system confirms that ·OH, SO4·− and 1O2 are the main ROS for CIP degradation. This study highlights the creation of miniature hypoxic space to regulate the content of O vacancies in ε-MnO2, providing a new idea for the synthesis of other O-vacancy-rich materials.

Abstract The combination of immune checkpoint inhibitors (ICIs) with chemotherapy (chemoimmunotherapy) in the neoadjuvant setting have achieved favorable clinical benefits in non-small cell lung cancer (NSCLC), but the mechanism of clinical responses remain unclear. We provide a rich resource of 186,477 individual immune cells from 48 samples of four treatment-naive and eight neoadjuvant chemoimmunotherapy treated IIIA NSCLC patients (responders versus non-responders) by single-cell RNA-seq and TCR-seq. We observed the synergistic increase of B cells and CD4+ T cells were associated with a positive therapeutic response of neoadjuvant chemoimmunotherapy. B cell IgG subclasses IgG1 and IgG3 played a critical role in anti-tumor immune response in tumor lesions, and this process was driven by increased IL-21 secreted by infiltrated T follicular helper (Tfh) cells after neoadjuvant chemoimmunotherapy. Furthermore, we uncovered several critical events for positive clinical outcomes, including the diminished activated TNFRSF4+ regulatory T cells (Tregs), increased LAMP3+ dendritic cells (DCs), and the expansion of intratumoral CD4+ T clones and peripheral C3-Cytotoxic CD8+ T clones. A validation cohort of 26 treatment-naive and 30 neoadjuvant chemoimmunotherapy treated IIIA/ IIIB NSCLC patients verified these findings. In total, our comprehensive study of the single-cell profile of immune cells provides insights into mechanisms underlying anti-PD-1-based therapies and identified potential predictive factors and therapeutic targets for improving the efficiency of neoadjuvant chemoimmunotherapy in NSCLC.

Abstract Background Trisporic acids are considered to be key regulators of carotenoid biosynthesis and sexual reproduction in zygomycetes, but the mechanisms underlying this regulation have not been fully elucidated. Results In this study, the relationships between trisporic acids and lycopene synthesis were investigated in Blakeslea trispora. The lycopene concentration in single fermentation by the (−) strain with the addition of 24 μg/L trisporic acids was slightly higher than that observed in mated fermentation. After transcriptomic analysis, a steroid 5α-reductase-like gene, known as SR5AL in B. trispora, was first reported. 5α-Reductase inhibitors reduced lycopene biosynthesis and downregulated the expression of sex determination and carotenoid biosynthesis genes. Overexpression of the SR5AL gene upregulated these genes, regardless of whether trisporic acids were added. Conclusion These findings indicated that the SR5AL gene is a key gene associated with the response to trisporic acids.

In artificial neural networks, the fourth passive element memristor can be utilized as an electronic synapse that serves as the interface between neurons. The artificial neuron composed of the memristor bridge synapse not only has the characteristics of low power consumption and high integration but also has a more simplified circuit and weight change conditions. Particularly, it has the ability of bionic intelligent information processing. This paper established two novel synaptic structures on the basis of memristor bridges (type 1 and type 2) and then synthetically analyzed how to realize the artificial neuron perceptron. Herein, the artificial synapses (type 1 and type 2) have the following characteristics: continuous changes in synaptic weights (positive, negative, and zero) and memory properties. Among them, the type 2 memristor bridge has the advantage of a wider range of weight updates for the synaptic circuit, which can realize the function of the artificial neuron perceptron with less error. This work lays the foundation for the future exploitation of artificial intelligence.

Abstract Background To determine the efficacy and safety of umbilical cord-derived mesenchymal stem cells (UC-MSCs) in Chinese adults with type 2 diabetes mellitus (T2DM). Methods In this single-center, double-blinded, randomized, placebo-controlled phase II trial, 91 patients were randomly assigned to receive intravenous infusion of UC-MSCs (n = 45) or placebo (n = 46) three times with 4-week intervals and followed up for 48 weeks from October 2015 to December 2018. The primary endpoint was the percentage of patients with glycated hemoglobin (HbA1c) levels of

Deep shale gas (3500–4500 m) is the important replacement field of shale gas production growth in the future China. The research on key parameters of deep shale-gas reservoir is critical to determine its basic geological characteristics and establish a suitable development mode. In order to clarify the adsorption characteristics and controlling factors of deep shale gas in Longmaxi Formation, the main tests such as high-pressure methane adsorption, low-temperature nitrogen and carbon dioxide adsorption coupled with the adsorption fitting model and comparative analysis were conducted. The results show that the adsorption isotherms of deep shale gas also have a downward trend in spite of the higher pressure, and there is no obvious difference in adsorption characteristics, which is mainly due to the similar characteristics of microscopic pore-structure between deep shale and shallower shale. It is found that different adsorption models can well fit the experimental adsorption curve of deep shale gas, but the absolute adsorption capacity converted from excess adsorption capacity shows the same fitting result, i.e., DA-LF model > DR model > Langmuir model. Furthermore, DR model based on micropore filling theory is more suitable for characterizing the adsorption law of deep shale gas combined with the correlation analysis between pore structure and adsorbed-gas capacity. In addition, TOC is the key material factor controlling the adsorption capacity, and specific surface area of micropore is the key spatial factor. Compared to shallower shale, the deep shale shows higher siliceous content, lower calcite content, lower TOC content and lower adsorbed-gas content (the proportion of adsorbed-gas is about 30%).

The application of melatonin (MT) has been shown to improve the quality during the storage of fruits and vegetables. The primary objective of this study is to investigate the effects of MT on the quality of fresh-cut Gastrodia elata during low-temperature (4 °C) storage. The results indicated that MT treatment not only suppressed the respiratory rate and malondialdehyde content but also slowed down the decline in total acidity and total soluble solids, effectively inhibiting microbial growth and enhancing the product safety of fresh-cut G. elata. The treatment with MT reduced the superoxide anions and hydrogen peroxide production, as well as inhibiting the activity and expression of peroxidase and polyphenol oxidase. Additionally, it led to increased activity and the expression of antioxidant-related enzymes, including superoxide dismutase, catalase, ascorbate peroxidase, glutathione reductase, monodehydroascorbate reductase, and dehydroascorbate reductase, while also resulting in elevated levels of ascorbic acid and glutathione. Furthermore, the treatment with MT induced an increase in the total phenolic and flavonoid content of fresh-cut G. elata and enhanced the activity and expression of key enzymes involved in the phenylpropanoid pathway (phenylalanine ammonia-lyase, cinnamate-4-hydroxylase, 4-coumarate: CoA ligase). In summary, MT enhances the antioxidant capacity by activating both the ROS metabolism and phenylpropanoid pathway, thus maintaining the quality of fresh-cut G. elata.

Memristors have been favored in artificial intelligence, neural networks, and chaotic circuits, especially in neural synapses because of their unique advantages such as resistance variability, nonvolatile nature, and nanometer size. Benefits such as integration scale and low power consumption contribute toward simulating the biological synaptic function. Compared with memory association circuits using traditional CMOS transistors, memristors will reduce the complexity of the circuit and the power consumption. Therefore, it is greatly promising to use memristors as synapses to construct neural networks to mimic human brain functions. This paper successfully establishes a recognition circuit based on memristors to recognize some characteristics (size, color, shape, and smooth) of fruits, which is a learning function. After a few seconds, the output signal voltage drops, and this is a forgetting function. Through the establishment of a recognition circuit, the neural network and human complex behavior were simulated. This work lays the foundation for further research of human neural networks.

Background: Renshen-Fuzi herb pair (RS-FZ) is often used in the clinical treatment of heart failure (HF) and has a remarkable therapeutic effect. However, the mechanism of RS-FZ for treating HF remains unclear. In our study, we explored the mechanism of RS-FZ for treating HF.Methods: Evaluation of RS-FZ efficacy by cardiovascular pharmacology. Moreover, Global metabolomics profiling of the serum was detected by UPLC-QTOF/MS. Multivariate statistics analyzed the specific serum metabolites and corresponding metabolic pathways. Combining serum metabolomics with network pharmacology, animal experiments screened and validated the critical targets of RS-FZ intervention in HF.Results: RS-FZ significantly ameliorated myocardial fibrosis, enhanced cardiac function, and reduced the serum HF marker (brain natriuretic peptide) level in rats with HF. Through topological analysis of the “Metabolite-Target-Component” interaction network, we found that 79 compounds of RS-FZ directly regulated the downstream specific serum metabolites by acting on four critical target proteins (CYP2D6, EPHX2, MAOB, and ENPP2). The immunohistochemistry results showed that RS-FZ observably improved the expression of CYP2D6 and ENPP2 proteins while decreasing the expression of EPHX2 and MAOB proteins dramatically.Conclusion: The integrated cardiovascular pharmacological assessment with serum metabolomics revealed that RS-FZ plays a crucial role in the treatment of HF by intervening in CYP2D6, EPHX2, MAOB, and ENPP2 target proteins. It provides a theoretical basis for RS-FZ for treating HF.

Altered glycan levels in serum have been associated with increased risk of cancer. In this study, we have developed and validated a HPLC-based method to analyze monosaccharide composition (D-mannose, Glucosamine, Galactosamine, Glucuronic acid, D-glucose, D-galactose, D-xylose, L-fucose) in human serum, with L-rhamnose, being used as internal standard. Monosaccharides obtained from hydrolyzed serum samples were derivatized by 1-Phenyl-3-methyl-5-pyrazolone. A ZORBAX XDB-C18 column(150×4.6mm) was used for chromatographic separation with 100 mM ammonium acetate buffer (NH4Ac-HAc, PH=5.5, solvent A), acetonitrile (ACN, solvent B) as a mobile phase. The calibration standard curves for the eight monosaccharides showed good linearity over the range of 2.5-500μg/mL with R2 > 0.995. The relative standard deviation values for intra-day and inter-day precision were ≤ 5.49%. Recovery was 69.01-108.96%. We observed that this column exhibited high specificity and selectivity to separate monosaccharides from serum. This method was then applied to quantitatively analyze the serum monosaccharide levels in 30 patients with endometrial cancer and 30 matched healthy controls. Statistical analysis indicated that the serum monosaccharide levels were significantly higher in patients compared with healthy controls (P value< 0.0001). Overall, we report here a simple, reliable, low-cost, and reproducible HPLC method for the separation and quantification monosaccharides in the human serum, which has potential value to serve as a screening marker for endometrial cancer.

ObjectivesTo examine the impact of demographic, socioeconomic, and behavioral factors on the development of cardiometabolic multimorbidity and mortality in Chinese elders.MethodsData from the Chinese Longitudinal Healthy Longevity Survey (CLHLS) 2002–2018 was used in the study. Cardiometabolic multimorbidity was defined as the presence of two or more cardiometabolic disorders, such as hypertension, diabetes, cardiovascular disease (CVD), heart disease, or stroke. Cox regression model and multi-state Markov model were developed to evaluate the association of the study factors with the progression of cardiometabolic conditions and mortality. The outcomes included three states (first cardiometabolic disease, cardiometabolic multimorbidity, and all-cause mortality) and five possible transitions among the three states.ResultsOf the 13,933 eligible individuals, 7,917 (56.8%) were female, and 9,540 (68.50%) were over 80 years old. 2,766 (19.9%) participants had their first cardiometabolic disease, 975 (7.0%) participants suffered from cardiometabolic multimorbidity, and 9,365 (67.2%) participants died. The progression to cardiometabolic multimorbidity was positively associated with being female (HR = 1.42; 95%CI, 1.10 − 1.85), living in the city (HR = 1.41; 95%CI, 1.04 − 1.93), overweight (HR = 1.43; 95%CI, 1.08 − 1.90), and obesity (HR = 1.75; 95% CI, 1.03 − 2.98). A higher risk for the first cardiometabolic disease was associated with being female (HR = 1.26; 95% CI, 1.15 − 1.39), higher socioeconomic status (SES, HR = 1.17; 95%CI, 1.07 − 1.28), lack of regular physical activity (HR = 1.13; 95%CI, 1.04 − 1.23), smoking (HR = 1.20; 95%CI, 1.08 − 1.33), ≤ 5 h sleep time (HR = 1.15; 95%CI, 1.02 − 1.30), overweight (HR = 1.48; 95% CI, 1.32 − 1.66), and obesity (HR = 1.34; 95%CI, 1.06 − 1.69). It also should be noted that not in marriage, lower SES and unhealthy behavioral patterns were risk factors for mortality.ConclusionThis study emphasized the importance of lifestyle and SES in tackling the development of cardiometabolic conditions among Chinese elders and provided a reference for policy-makers to develop a tailored stage-specific intervention strategy.

Twin-row ridge cultivation is widely used in soybean planting in northern China. In order to find the optimal parameters of soybean agronomy, the twin-row planter with subsoiling was designed. Field experiments were conducted to evaluate the effects of plant arrangements and cluster densities on soybean growth and grain yield under different tillage treatments. The experiment used a randomised complete block design consisting of 20 treatments in a 2×2×5 factorial arrangement. Two tillage treatments were inter-row subsoiling and no subsoiling. Each tillage treatment included the combination of plant arrangements (side-by-side arrangement and triangular arrangement) and cluster densities (one, two, three, four, and five plants). The variables measured included soil moisture content, seedling emergence, biomass accumulation and allocation, and grain yield. We have concluded that the performance of inter-row subsoiling treatment was much better than that of no subsoiling treatment. Meanwhile, the triangular arrangement and two plants per cluster were the best choices for soybean biomass accumulation and grain yield in northern China. This study provided a reference for the innovative design of the twin-row planter with subsoiling and the optimisation of soybean agronomy.

An appropriate contact mechanics model and parameters are key to achieving accurate results in discrete element analyzis. This is necessary to predict the process of contact collision between the soil and maize seed during deposition. In this paper, the contact process between maize seed and soil is analyzed using the maize seed variety (Liangyu 99) and maize-sowing field soil (with three different moisture contents) as research objects. Based on this, the contact process between maize seeds and soil has been analyzed, on the basis of which a mechanical model suitable for simulating the contact process between maize seeds and soil has been explored, and the selection of parameters between heterogeneous particles (maize seed and soil particles) has been investigated. The results showed that adhesion forces have a significant effect on the collision process between seed and soil particles. While the presence of tangential adhesion force can be replaced by increasing the static and rolling friction coefficients, the normal adhesion force cannot be compensated in this way. The Edinburgh Elasto-Plastic Adhesive (EEPA) model is selected in this paper to describe the contact between seed and soil particles. The significance of the input parameters in the EEPA model is investigated using the Plackett–Burman test. The parameters between soil and seed particles are optimized using the central composite design, and the optimal parameter combinations are obtained. The relative error between the simulation and test result of the slope test for the three soil moisture contents is within 5.4%, validating the accuracy of the calibrated parameters.

Cracks in building facades are inevitable due to the age of the building. Cracks found in the building facade may be further exacerbated if not corrected immediately. Considering the extensive size of some buildings, there is definitely a need to automate the inspection routine to facilitate the inspection process. The incorporation of deep learning technology for the classification of images has proven to be an effective method in many past civil infrastructures like pavements and bridges. There is, however, limited research in the built environment sector. In order to align with the Smart Nation goals of the country, the use of Smart technologies is necessary in the building and construction industry. The focus of the study is to identify the effectiveness of deep learning technology for image classification. Deep learning technology, such as Convolutional Neural Networks (CNN), requires a large amount of data in order to obtain good performance. It is, however, difficult to collect the images manually. This study will cover the transfer learning approach, where image classification can be carried out even with limited data. Using the CNN method achieved an accuracy level of about 89%, while using the transfer learning model achieved an accuracy of 94%. Based on this, it can be concluded that the transfer learning method achieves better performance as compared to the CNN method with the same amount of data input.

The epitaxial growth of high-index Fe films on MgO(113) substrates is successfully achieved using direct current (DC) magnetron sputtering, despite the significant lattice constant mismatch between Fe and MgO. X-ray diffraction (XRD) analysis is employed to characterize the crystal structure of Fe films, revealing an Fe(103) out-of-plane orientation. Furthermore, our investigation reveals that the Fe[010] direction is parallel to the MgO[11¯0] direction within the films plane. These findings provide valuable insights into the growth of high-index epitaxial films on substrates with large lattice constant mismatch, thereby contributing to the advancement of research in this field.

The prevalence of liver disease has been increasing worldwide. Moreover, the burden of end-stage liver disease, including cirrhosis and liver cancer, is high because of high mortality and suboptimal treatment. The pathological process of liver disease includes steatosis, hepatocyte death, and fibrosis, which ultimately lead to cirrhosis and liver cancer. Clinical and preclinical evidence indicates that non-neoplastic liver diseases, particularly cirrhosis, are major risk factors for liver cancer, although the mechanism underlying this association remains unclear. Yes-associated protein (YAP) and transcriptional coactivator with PDZ-binding motif (TAZ) are transcriptional activators that regulate organ size and cancer development. YAP and TAZ play important roles in liver development, regeneration, and homeostasis. Abnormal YAP and TAZ levels have also been implicated in non-neoplastic liver diseases (e.g., non-alcoholic fatty liver disease, alcoholic liver disease, liver injury, and liver fibrosis). Here, we review recent findings on the roles of YAP and TAZ in non-neoplastic liver diseases and discuss directions for future research. This review provides a basis for the study of non-neoplastic liver diseases.

For accurate estimation of the thermal characteristics of AlGaN/GaN high electron mobility transistors on diamond, the anisotropic thermal conductivities of polycrystalline diamond (PCD) and GaN with their thickness dependences are included in the finite element simulation model, of which the PCD thermal conductivity kPCD is calculated through detailed analyses of the grain sizes in the directions parallel and vertical to the interface and relative phonon-grain boundary scatterings. In addition, the GaN thermal conductivity kGaN is the result of an approximate solution of the phonon Boltzmann transport equation. To investigate the effects of anisotropic GaN and PCD thermal conductivity, four cases of thermal conductivity models are compared: anisotropic kGaN and kPCD, anisotropic kGaN with constant kPCD, constant kGaN with anisotropic kPCD, and constant kGaN and kPCD. Then the maximum channel temperature simulated from the four above-mentioned models and the errors relative to the case of anisotropic kGaN and kPCD are investigated with respect to thermal boundary resistance, GaN layer thickness, gate pitch, heat source width, and power dissipation. The results show that the combination of anisotropic kPCD and constant kGaN overestimates the maximum channel temperature rise, and using constant kPCD and anisotropic kGaN underestimates the maximum channel temperature rise for almost all the simulations in the text. However, the channel temperature stemming from constant kPCD and kGaN is generally closer to the case with anisotropic PCD and GaN thermal conductivity.

Abstract Background CITED4 belongs to the CBP/p300‐interacting transactivator with glutamic acid and aspartic acid‐rich tail (CITED) family which is induced by various cytokines and participates in cytokine‐induced proliferation and differentiation. CITED4 is induced by HB‐EGF in lung cancer cells. However, it is unclear whether and how CITED4 contributes to the invasion and metastasis of lung adenocarcinoma (ADC). Methods CITED4 expression in lung adenocarcinoma and its association with disease‐free survival (DFS) and overall survival were analyzed based on a cohort of 261 patients. The roles of CITED4 were validated via loss‐of‐function and gain‐of‐function experiments. The relationship between CITED4 and CLDN3 was validated by immunohistochemistry, Western blotting, and luciferase reporter assays. The function of the CITED4‐CTNNB1‐CLDN3 complex was fully validated and described. Results CITED4 expression was significantly upregulated in ADC tissues and cells and a predictor for DFS. Downregulation of CITED4 attenuated the proliferation and invasion, whereas CITED4 overexpression enhanced these effects. Overexpression and knockdown of CITED4 resulted in the upregulation and downregulation of CLDN3, respectively. Moreover, CITED4 downregulation suppressed CLDN3‐mediated ADC cell metastasis in vivo. CITED4 was highly expressed and positively correlated with CLDN3. Mechanistically, CITED4 interacted with CTNNB1 and functioned synergistically to enhance CLDN3 transcription. Importantly, CITED4 induced ADC invasion via a CLDN3‐dependent pathway. CITED4 determined the level of CLDN3, which in turn affected the sensitivity of tumors to Clostridium perfringens enterotoxin treatment. Conclusions The CITED4‐CTNNB1‐CLDN3 axis plays a key role in the invasion and metastasis of ADC and provides a novel therapeutic target for lung cancer treatment.