25 results on '"Yuhong Ye"'
Search Results
2. Effects of a novel ANLN E841K mutation associated with SRNS on podocytes and its mechanism
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Li Lin, Yuhong Ye, Haidong Fu, Weizhong Gu, Manli Zhao, Jingmiao Sun, Zhongkai Cao, Guoping Huang, Yi Xie, Fei Liu, Lu Li, Qiuyu Li, Jianhua Mao, and Lidan Hu
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ANLN mutation ,SRNS ,Podocyte ,Podocyte-specific knockout mouse ,Medicine ,Cytology ,QH573-671 - Abstract
Abstract Background Steroid-resistant nephrotic syndrome (SRNS) is characterized by unrelieved proteinuria after an initial 4–8 weeks of glucocorticoid therapy. Genes in podocytes play an important role in causing SRNS. Objective This study aimed to report a pathogenic mutation in SRNS patients and investigate its effects on podocytes, as well as the pathogenic mechanism. Methods We screened out a novel mutation by using whole-exon sequencing in the SRNS cohort and verified it via Sanger sequencing. Conservative analysis and bioinformatic analysis were used to predict the pathogenicity of the mutation. In vitro, stable podocyte cell lines were constructed to detect the effect of the mutation on the function of the podocyte. Moreover, an in vivo mouse model of podocyte ANLN gene knockout (ANLN podKO) was used to confirm clinical manifestations. Transcriptome analysis was performed to identify differential gene expression and related signaling pathways. Results ANLN E841K was screened from three unrelated families. ANLN E841K occurred in the functional domain and was predicted to be harmful. The pathological type of A-II-1 renal biopsy was minimal change disease, and the expression of ANLN was decreased. Cells in the mutation group showed disordered cytoskeleton, faster cell migration, decreased adhesion, increased endocytosis, slower proliferation, increased apoptosis, and weakened interaction with CD2 association protein. ANLN podKO mice exhibited more obvious proteinuria, more severe mesangial proliferation, glomerular atrophy, foot process fusion, and increased tissue apoptosis levels than ANLN flox/flox mice after tail vein injection of adriamycin. Upregulated differentially expressed genes in cells of the mutation group were mainly enriched in the PI3K-AKT pathway. Conclusion The novel mutation known as ANLN E841K affected the function of the ANLN protein by activating the PI3K/AKT/mTOR/apoptosis pathway, thus resulting in structural and functional changes in podocytes. Our study indicated that ANLN played a vital role in maintaining the normal function of podocytes. Video Abstract
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- 2023
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3. Comprehensive overview of genotype distribution and prevalence of human papillomavirus in cervical lesions
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Yang Liu, Chengquan Zhao, Tiannan Wang, Yuhong Ye, Terrel Jones, and Xianxu Zeng
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Gynecology and obstetrics ,RG1-991 - Abstract
Across cervical squamous and glandular lesions, a spectrum of human papillomavirus (HPV) genotypes has been identified. This review aims to provide a comprehensive summary detailing the distribution and profile of HPV genotypes detected in cervical lesions, leveraging insights from histological and cytological findings. High-risk HPV (HR-HPV) genotypes exhibit varying degrees of oncogenic potential, with HPV16 and HPV18 identified as the most prevalent and oncogenic types. The distribution of HR-HPV genotypes varies among different degrees of the cervical lesions and varies between squamous and glandular neoplasia. HPV16 is predominantly associated with severe lesions (precancers and carcinomas), while HPV18 demonstrates a significantly higher prevalence in endocervical as compared with squamous neoplasia. The distribution of HR-HPV in severe squamous lesions is complex, involving many HR-HPV genotypes in addition to HPV16, while the distribution of HR-HPV genotypes in endocervical glandular lesions is mainly limited in HPV18 and HPV16.Large datasets from China have identified the three most common HR-HPV genotypes in this population as stratified by diagnostic category: HPV52, HPV16, HPV58 in histologically negative cases and cervical intraepithelial neoplasia 1 (CIN1); HPV16, HPV52, HPV58 in CIN2/3; HPV16, HPV58, HPV52 or HPV18 in squamous cell carcinoma (SCC); HPV16, HPV18 and HPV52 in endocervical adenocarcinoma in situ (AIS), invasive adenocarcinoma, as well as mixed squamous and glandular lesions. HPV33 is the fourth most common HPV type in CIN2/3 and SCC, while HPV45 occurs more commonly in AIS and adenocarcinoma, compared with squamous lesions. The prevalence and distribution of multiple HR-HPV coinfections vary across different cervical diseases. The clinical significance and pathogenesis of these multiple HR-HPV infections remain uncertain, although recent two large studies demonstrate that multiple HR-HPV infections are not associated with cumulatively higher risk of high-grade cervical squamous lesion development, suggesting competitive and/or cooperative interactions among HPV genotypes. Extensive HPV genotyping aids in risk assessment and optimising clinical approaches for women with mild abnormalities in Pap cytology. Women with atypical squamous cells of undetermined significance (ASC-US) and low-grade squamous intraepithelial lesion (LSIL) Pap test results and with the infection of some HR-HPV genotypes carry a very low risk of high-grade cervical lesions. HPV genotyping can allow for risk stratification and triage optimisation for these HR-HPV-positive women. Women with atypical glandular cell (AGC) Pap test results showed a specific HPV genotyping pattern and extended HPV genotyping may be helpful for the clinical management of AGCs. Continual advancements in clinical guidelines integrating extended genotyping would increase diagnostic accuracy and refine strategies in clinical management.
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- 2024
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4. A Clinicopathological and Molecular Analysis of Sellar/Suprasellar Neurocytoma Mimicking Pituitary Adenoma
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Lifeng Zhang, Weiwei Fu, Limei Zheng, Fangling Song, Yupeng Chen, Changzhen Jiang, Zhen Xing, Chengcong Hu, Yuhong Ye, Sheng Zhang, Xiaorong Yan, and Xingfu Wang
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neurocytoma ,extraventricular neurocytoma ,sellar and suprasellar region ,pituitary tumor ,clincopathology ,Diseases of the endocrine glands. Clinical endocrinology ,RC648-665 - Abstract
ObjectiveTo investigate the clinicopathological characteristics, molecular genetic characteristics and prognosis of extraventricular neurocytoma located in the sellar/suprasellar region.MethodsSeven archived tumor samples derived from 4 patients with neurocytoma in the sellar/suprasellar region were collected from the First Affiliated Hospital of Fujian Medical University and the Affiliated Hospital of Qingdao University and retrospectively analyzed for clinical manifestations, imaging features, and histopathological features. Neuronal and pituitary biomarkers and molecular features were detected in these tumor tissues by immunohistochemistry and FISH or Sanger sequencing. The related literature was reviewed.ResultsThree patients were female, while 1 was male, with an average age of 35.5 years (range: 27 to 45 years). The initial manifestations were mainly headache and blurred vision in both eyes. The first MRI examination showed marginally enhancing masses in the intrasellar or intra- to suprasellar region. The diagnosis of pituitary adenomas was based on imaging features. The levels of pituitary hormones were normal. Histologically, the tumor cells were arranged in a sheet-like, monotonous architecture and were uniform in size and shape with round to oval, exquisite and hyperchromatic nuclei, which densely packed close to one another and were separated only by a delicate neuropil background. There was no evident mitosis, necrosis or microvascular proliferation. The three cases of recurrent tumors were highly cellular and showed increased mitotic activity. Immunohistochemically, the tumor cells were positive for syn, CR, CgA, and vasopressin and were focally positive for NeuN, TTF-1, NF, CK8, vimentin, and S100 proteins. Other markers, including IDH1, BRAF VE1, Olig-2, and EMA, were negative. Pituitary transcription factors and anterior pituitary hormones were negative. Molecular genetic testing showed that the tumor cells lacked IDH gene mutations, LOH of 1p/19q, MYCN amplification, and EGFR alteration. With a median follow-up of 74.5 months (range 23 to 137 months), 3 patients relapsed at 11, 50, and 118 months after the initial surgery.ConclusionThe morphological features and immunophenotypes of neurocytoma in the sellar/suprasellar region are similar to those of classic central neurocytoma. The prognosis is relatively good. Gross-subtotal resection and atypical subtype may be related to tumor recurrence.
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- 2022
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5. Case report: a Chinese girl with dent disease 1 and turner syndrome due to a hemizygous CLCN5 gene mutation and Isochromosome (Xq)
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Yuhong Ye, Jingjing Wang, Xiaofang Quan, Ke Xu, Haidong Fu, Weiyue Gu, and Jianhua Mao
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Dent disease 1 ,CLCN5 ,Turner syndrome ,I(X)(q10) ,LMWP ,Diseases of the genitourinary system. Urology ,RC870-923 - Abstract
Abstract Background Female Dent disease 1 patients with low-molecular-weight proteinuria (LMWP) due to CLCN5 gene mutation were rarely reported, and these cases that the people were also with Turner syndrome (TS) were even hardly documented before. Case presentation Here we report a 3-year and 11-month old Chinese girl with short stature who had a karyotype of 46,X,i(X)(q10) and a de novo pathogenic variant in the CLCN5 gene on the short arm of X chromosome. Laboratory examinations showed that the patient had LMWP, hypercalciuria, hypophosphatemia, delayed bone age, and genital dysplasia. Conclusion The combination of i(X)(q10) and CLCN5 mutation causes the deletion of the wild-type CLCN5 allele that results in Dent-1 and TS. To the best of our knowledge, this is the first case that a female CLCN5 mutation hemizygote is diagnosed with Dent-1 and Turner syndrome due to isochromosome X. Also, our case has indicated that the prevalence of the situation may be largely underestimated because of the mild signs of females with Dent-1.
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- 2020
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6. Protein phosphatase 2A modulates podocyte maturation and glomerular functional integrity in mice
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Xiujuan Zhu, Yuhong Ye, Chengxian Xu, Cunji Gao, Yingying Zhang, Jing Zhou, Weiqiang Lin, and Jianhua Mao
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PP2A ,Dephosphorylation ,Podocyte ,Proteinuria ,Glomerulopathy ,YB-1 ,Medicine ,Cytology ,QH573-671 - Abstract
Abstract Background Protein phosphorylation & dephosphorylation are ubiquitous cellular processes that allow for the nuanced and reversible regulation of protein activity. Protein phosphatase 2A (PP2A) is a multifunction phosphatase that is well expressed in all cell types of kidney during early renal development, though its functions in kidney remains to be elucidated. Methods PP2A conditional knock-out mice was generated with PP2A fl/fl mice that were crossed with Podocin-Cre mice. The phenotype of Pod-PP2A–KO mice (homozygous for the floxed PP2A allele with Podocin-Cre) and littermate PP2A fl/fl controls (homozygous for the PP2A allele but lacking Podocin-Cre) were further studied. Primary podocytes isolated from the Pod-PP2A-KO mice were cultured and they were then employed with sing label-free nano-LC − MS/MS technology on a Q-exactive followed by SIEVE processing to identify possible target molecular entities for the dephosphorylation effect of PP2A, in which Western blot and immunofluorescent staining were used to analyze further. Results Pod-PP2A–KO mice were developed with weight loss, growth retardation, proteinuria, glomerulopathy and foot process effacement, together with reduced expression of some slit diaphragm molecules and cytoskeleton rearrangement of podocytes. Y box protein 1 (YB-1) was identified to be the target molecule for dephosphorylation effect of PP2A. Furthermore, YB-1 phosphorylation was up-regulated in the Pod-PP2A–KO mice in contrast to the wild type controls, while total and un-phosphorylated YB-1 both was moderately down-regulated in podocytes from the Pod-PP2A-KO mice. Conclusion Our study revealed the important role of PP2A in regulating the development of foot processes and fully differentiated podocytes whereas fine-tuning of YB-1 via a post-translational modification by PP2A regulating its activity might be crucial for the functional integrity of podocytes and glomerular filtration barrier. Graphic abstract
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- 2019
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7. Value of contrast-enhanced ultrasound for detection of synovial vascularity in experimental rheumatoid arthritis: an exploratory study
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Hui Liu, Chao Huang, Shuqiang Chen, Qing Zheng, Yuhong Ye, Zhen Ye, and Guorong Lv
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Medicine (General) ,R5-920 - Abstract
Objective This study aimed to examine the associations between contrast-enhanced ultrasound (CEUS) imaging and synovial hypervascularity and synovitis score in a rabbit model of antigen-induced arthritis (AIA), compared with power Doppler ultrasound (PDUS). Methods We investigated 50 knee joints in 25 AIA rabbits (AIA group), and 10 knee joints in five sham-injected rabbits (control group). PDUS and CEUS images were evaluated at the 8 th week. Ultrasound-guided synovial biopsies were targeted in the area with hypervascularity, and synovial microvessel density (MVD) was evaluated by immunohistochemical staining of CD31. Results The PDUS score was significantly higher in the AIA group (2.61 ± 0.78) compared with the control group (0.50 ± 0.53). CEUS in the AIA group revealed a fast-in/slow-out pattern of contrast enhancement. MVD revealed by CD31+ vessel count and the synovitis score were significantly higher in the AIA group compared with the control group. In the AIA group, CEUS findings showed a better correlation with MVD revealed by CD31+ and synovitis score than PDUS findings. Conclusion CEUS is superior to PDUS for estimating synovial hypervascularity and hyperplasia in experimental rheumatoid arthritis.
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- 2019
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8. Histological characterisation and prognostic evaluation of 62 gastric neuroendocrine carcinomas
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Yujie Deng, Xiaohui Chen, Yuhong Ye, Xi Shi, Kunshou Zhu, Liming Huang, Sheng Zhang, Mingang Ying, and Xuede Lin
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neuroendocrine carcinoma ,stomach ,prognosis ,Ki-67 ,chromogranin A ,Medicine - Abstract
Aim of the study: To determine the significance of expression of synaptophysin, chromogranin A, and Ki-67 and their association with clinicopathological parameters, and to find out the possible prognostic factors in gastric neuroendocrine carcinoma (G-NEC). Material and methods: We investigated the immunohistochemical features and prognosis of 62 G-NECs, and evaluated the association among expressions of synaptophysin, chromogranin A, and Ki-67, clinicopathological variables, and outcome. Results : Chromogranin A expression was found more commonly in small-cell NECs (9/9, 100%) than in large-cell NECs (27/53, 51%) (p = 0.008). No statistical significance was found in Ki-67 (p = 0.494) or synaptophysin (p > 0.1) expression between NEC cell types. Correlation analyses revealed that Ki-67 expression was significantly associated with mid-third disease of stomach (p = 0.005) and vascular involvement (p = 0.006), and hada trend of significant correlation with tumour relapse (p = 0.078). High expression of chromogranin A was significantly associated with histology of small-cell NECs (p = 0.008) and lesser tumour greatest dimension (p = 0.038). The prognostic significance was determined by means of Kaplan-Meier survival estimates and log-rank tests, and as a result, early TNM staging and postoperative chemotherapy were found to be correlated with longer overall survival (p < 0.05). Univariate analysis revealed associations between poor prognosis in NECs and several factors, including high TNM staging (p = 0.048), vascular involvement (p = 0.023), relapse (p = 0.004), and microscopic/macroscopic residual tumour (R1/2, p < 0.001). In a multivariate analysis, relapse was identified as the sole independent prognostic factor. Conclusions : No significant correlation between survival and expression of synaptophysin, chromogranin A, or Ki-67 has been determined in G-NECs. Our study indicated that early diagnosis, no-residual-tumour resection, and postoperative chemotherapy were possible prognostic factors.
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- 2016
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9. Scalable and Controllable Synthesis of Pt‐Ni Bunched‐Nanocages Aerogels as Efficient Electrocatalysts for Oxygen Reduction Reaction
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Yuanyuan Zheng, Amanda Schramm Petersen, Hao Wan, René Hübner, Jiangwei Zhang, Jianlan Wang, Haoyuan Qi, Yuhong Ye, Chaolun Liang, Jing Yang, Zhiming Cui, Yuezhong Meng, Zhikun Zheng, Jan Rossmeisl, and Wei Liu
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Renewable Energy, Sustainability and the Environment ,General Materials Science - Published
- 2023
10. PP2A protects podocytes against Adriamycin-induced injury and epithelial-to-mesenchymal transition via suppressing JIP4/p38-MAPK pathway
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Haidong Fu, Xiujuan Zhu, Zhihong Lu, Yuhong Ye, and Jianhua Mao
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MAPK/ERK pathway ,biology ,Chemistry ,p38 mitogen-activated protein kinases ,Clinical Biochemistry ,Biomedical Engineering ,Bioengineering ,macromolecular substances ,Cell Biology ,Protein phosphatase 2 ,Cell biology ,Podocyte ,Nephrin ,medicine.anatomical_structure ,Apoptosis ,embryonic structures ,biology.protein ,medicine ,Original Article ,Epithelial–mesenchymal transition ,Protein kinase A ,Biotechnology - Abstract
Protein phosphatase 2A (PP2A) is one of the major protein serine/threonine phosphatases (PPPs) with regulatory effects on several cellular processes, but its role and function in Adriamycin (ADR)-treated podocytes injury needs to be further explored. Mice podocytes were treated with ADR and PP2A inhibitor (okadaic acid, OA). After transfection, cell apoptosis was detected by flow cytometry. Expressions of podocytes injury-, apoptosis- and epithelial-to-mesenchymal transition (EMT)- and JNK-interacting protein 4/p38-Mitogen-Activated Protein Kinase (JIP4/p38-MAPK) pathway-related factors were measured using quantitative real-time polymerase chain reaction (qRT-PCR) and Western blot as needed. Interaction between PP2A and JIP4/MAPK pathway was confirmed using co-immunoprecipitation (Co-Ip) assay. In podocytes, ADR inhibited PP2A, Nephrin and Wilms' tumor (WT) 1 expressions yet upregulated apoptosis and Desmin expression, and suppressing PP2A expressionenhanced the effects. PP2A overexpression reversed the effects of ADR on PP2A and podocyte injury-related factors expressions and apoptosis of podocytes. JIP4 was the candidate gene interacting with both PP2A and p38-MAPK pathway, and PP2A overexpression alleviated the effects of ADR on p38-MAPK pathway-related factors expressions. Additionally, in ADR-treated podocytes, PP2A suppression enhanced the effects of ADR, yet silencing of JIP4 reversed the effects of PP2A suppression on regulating p38-MAPK pathway-, apoptosis- and EMT-related factors expressions and apoptosis, with upregulations of B-cell lymphoma-2 (Bcl-2) and E-cadherin and down-regulations of Bcl-2 associated protein X (Bax), cleaved (C)-casapse-3, N-cadherin, Vimentin and Snail. PP2A protects ADR-treated podocytes against injury and EMT by suppressing JIP4/p38-MAPK pathway, showing their interaction in podocytes.
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- 2021
11. Case report: a Chinese girl with dent disease 1 and turner syndrome due to a hemizygous CLCN5 gene mutation and Isochromosome (Xq)
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Jianhua Mao, Yuhong Ye, Weiyue Gu, Ke Xu, Xiaofang Quan, Jingjing Wang, and Haidong Fu
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0301 basic medicine ,medicine.medical_specialty ,Pediatrics ,Hypophosphatemia ,Turner syndrome ,Hypercalciuria ,Isochromosome ,Case Report ,Dent Disease ,030105 genetics & heredity ,Kidney ,Nephrolithiasis ,lcsh:RC870-923 ,Short stature ,Bone and Bones ,03 medical and health sciences ,Chloride Channels ,Internal medicine ,LMWP ,medicine ,Humans ,X chromosome ,Hemizygote ,I(X)(q10) ,Bone Development ,biology ,business.industry ,CLCN5 ,Ovary ,Uterus ,Dent disease 1 ,Genetic Diseases, X-Linked ,Bone age ,medicine.disease ,lcsh:Diseases of the genitourinary system. Urology ,Isochromosomes ,Proteinuria ,030104 developmental biology ,Nephrology ,Child, Preschool ,Mutation ,biology.protein ,Female ,medicine.symptom ,business - Abstract
Background Female Dent disease 1 patients with low-molecular-weight proteinuria (LMWP) due to CLCN5 gene mutation were rarely reported, and these cases that the people were also with Turner syndrome (TS) were even hardly documented before. Case presentation Here we report a 3-year and 11-month old Chinese girl with short stature who had a karyotype of 46,X,i(X)(q10) and a de novo pathogenic variant in the CLCN5 gene on the short arm of X chromosome. Laboratory examinations showed that the patient had LMWP, hypercalciuria, hypophosphatemia, delayed bone age, and genital dysplasia. Conclusion The combination of i(X)(q10) and CLCN5 mutation causes the deletion of the wild-type CLCN5 allele that results in Dent-1 and TS. To the best of our knowledge, this is the first case that a female CLCN5 mutation hemizygote is diagnosed with Dent-1 and Turner syndrome due to isochromosome X. Also, our case has indicated that the prevalence of the situation may be largely underestimated because of the mild signs of females with Dent-1.
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- 2020
12. Long noncoding RNA EIF1AX-AS1 promotes endometrial cancer cell apoptosis by affecting EIF1AX mRNA stabilization
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Chengyu Lv, Jiandong Sun, Yuhong Ye, Zihang Lin, Hua Li, Yue Liu, Kaien Mo, Weiwei Xu, Weitao Hu, Eman Draz, and Shie Wang
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Cancer Research ,Apoptosis ,General Medicine ,Internal Ribosome Entry Sites ,Xenograft Model Antitumor Assays ,Endometrial Neoplasms ,Gene Expression Regulation, Neoplastic ,Oncology ,Cell Line, Tumor ,Humans ,Female ,RNA, Long Noncoding ,RNA, Messenger ,Cell Proliferation - Abstract
Long noncoding RNAs (lncRNAs) have been found to play an important role in the occurrence and development of endometrial carcinoma (EC). Here, using RNA sequencing analysis, we systemically screened and identified the lncRNA eukaryotic translation initiation factor 1A, X-linked (EIF1AX)-AS1, which is aberrantly downregulated in clinical EC tissues and closely correlated with tumor type. EIF1AX-AS1 markedly inhibited EC cell proliferation and promoted apoptosis in vitro and in vivo. Mechanistically, EIF1AX-AS1 interacts with EIF1AX mRNA and poly C binding protein 1 (PCBP1), which promote EIF1AX mRNA degradation. Intriguingly, by interacting with internal ribosome entry site-related protein Y-box binding protein 1 (YBX-1), EIF1AX promotes c-Myc translation through the internal ribosome entry site pathway. c-Myc promotes EIF1AX transcription and thus forms a feed-forward loop to regulate EC cell proliferation. Taken together, these data reveal new insights into the biology driving EC proliferation and highlights the potential of lncRNAs as biomarkers for prognosis and future therapeutic targets for cancer.
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- 2022
13. XPO1-Mediated EIF1AX Cytoplasmic Relocation Promotes Tumor Migration and Invasion in Endometrial Carcinoma
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Yuhong Ye, Chengyu Lv, Jiandong Sun, Zihang Lin, Yue Liu, Yuxiu Huang, Yupeng Chen, Hua Li, Xiuli Lian, Xia Jiang, Sheng Zhang, and Shie Wang
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Cytoplasm ,Aging ,Article Subject ,Eukaryotic Initiation Factor-1 ,Receptors, Cytoplasmic and Nuclear ,Cell Biology ,General Medicine ,Biochemistry ,Endometrial Neoplasms ,Endometrium ,Cell Line, Tumor ,Endometrial Hyperplasia ,Humans ,Female ,Cell Proliferation - Abstract
Dysregulation of eukaryotic translation initiation factor 1A, X-linked (EIF1AX), has been implicated in the pathogenesis of some cancers. However, the role of EIF1AX in endometrial carcinoma (EC) remains unknown. We investigated the EIF1AX expression in EC patients and assessed its tumorigenesis-associated function and nucleocytoplasmic transport mechanism in vitro and in vivo. The results indicated that the cytoplasmic EIF1AX expression showed a gradual increase when going from endometrium normal tissue, simple endometrial hyperplasia, complex endometrial hyperplasia, and endometrial atypical hyperplasia to EC, while vice versa for the nuclear EIF1AX expression. In addition, the cytoplasmic EIF1AX expression was positively correlated with histologic type, high International Federation of Gynecology and Obstetrics (FIGO) grade, advanced FIGO stage, deeper infiltration, high Ki67 index, and shorter recurrence-free survival in EC patients. In vitro, short hairpin RNA-mediated EIF1AX depletion or SV40NLS-mediated EIF1AX import into the nucleus in multiple human EC cells potently suppressed cell migration and invasion, epithelial-mesenchymal transition, and lung metastasis. Moreover, exportin 1 induced the transport of EIF1AX from the nucleus to the cytoplasm that could be inhibited by leptomycin B treatment or the mutation in the EIF1AX location sequence. These results demonstrate that cytoplasmic EIF1AX may play a key role in the incidence and promotion of EC, and thus, targeting EIF1AX or its nucleocytoplasmic transport process may offer an effective new therapeutic approach to EC.
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- 2022
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14. LncRNA EIF1AX-AS1 Promotes Endometrial Cancer Cell Apoptosis via Interacting With PCBP1 to Facilitate EIF1AX mRNA Destabilization
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Chengyu Lv, Jiandong Sun, Yuhong Ye, Zihang Lin, Hua Li, Yue Liu, Kaien Mo, Weiwei Xu, Weitao Hu, Eman Draz, and Shie Wang
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- 2021
15. Longitudinal Changes in Knee Joint Synovial Vascularity in a Rabbit Model of Rheumatoid Arthritis: Quantification Using Power Doppler Ultrasound and Contrast-Enhanced Ultrasound
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Zhen Ye, Hui Liu, Qing Zheng, Shuqiang Chen, Yuhong Ye, Chao Huang, and Guorong Lyu
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Male ,Time Factors ,Acoustics and Ultrasonics ,Knee Joint ,Biophysics ,Arthritis ,Contrast Media ,030218 nuclear medicine & medical imaging ,Arthritis, Rheumatoid ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Vascularity ,Biopsy ,medicine ,Animals ,Radiology, Nuclear Medicine and imaging ,030203 arthritis & rheumatology ,Radiological and Ultrasound Technology ,medicine.diagnostic_test ,Neovascularization, Pathologic ,business.industry ,Ultrasound ,Synovial Membrane ,Ultrasonography, Doppler ,medicine.disease ,Image Enhancement ,Vascular endothelial growth factor ,Disease Models, Animal ,chemistry ,Rheumatoid arthritis ,Rabbits ,medicine.symptom ,business ,Nuclear medicine ,Contrast-enhanced ultrasound - Abstract
We studied a rabbit model of rheumatoid arthritis (RA) to examine the time course of changes in synovial neovascularization based on quantitative power Doppler ultrasound and contrast-enhanced ultrasound (CEUS). Twenty-five male New Zealand rabbits were in the ovalbumin-induced arthritis (OIA) group, and 5 were in the control group. Both rear knee joints of all rabbits were examined using conventional US and CEUS over 16 weeks. The knee synoviums of OIA rabbits were sampled by US-guided biopsy, and expression of CD31 and vascular endothelial growth factor (VEGF) was determined by immunohistochemistry. The correlation of joint damage based on multimodal US with microvessel density (CD31 positivity) and VEGF expression at different times was analyzed. OIA rabbits had increased synovial expression of CD31 and VEGF from weeks 6 to 12 (p < 0.01). During the early stage of CEUS enhancement, "dot enhancement" was more common at weeks 6 and 8, and "stripe enhancement" was more common at weeks 12 and 16 (p < 0.05). There were significant positive correlations of synovial CD31 and VEGF expression with power Doppler image grade, CEUS grade and peak intensity (p < 0.05 for all). Thus, OIA rabbits mimicked early-stage RA at 6 to 8 weeks, middle-stage RA at 8 to 12 weeks and late-stage RA at 12 to 16 weeks. Power Doppler image grade, CEUS grade and peak intensity, especially when combined with CD31 expression data, accurately characterized the extent of synovial vascularization in a rabbit model of RA. Increased vascularity based on CEUS may have value for the early diagnosis of RA.
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- 2020
16. Juvenile Granulosa Cell Tumors of the Ovary
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Ru Qian, Yuhong Ye, Chengyu Lv, Pengcheng Wang, Yupeng Chen, Shie Wang, and Songhua Xu
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Adult ,Pathology ,medicine.medical_specialty ,Ovary ,03 medical and health sciences ,0302 clinical medicine ,Medicine ,Precocious puberty ,Neoplasm ,Humans ,Yolk sac ,Ovarian follicle ,Child ,Granulosa Cell Tumor ,Ovarian Neoplasms ,030219 obstetrics & reproductive medicine ,business.industry ,Infant, Newborn ,Forkhead Transcription Factors ,General Medicine ,Abdominal distension ,medicine.disease ,Forkhead box L2 ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Mutation ,Immunohistochemistry ,Female ,medicine.symptom ,business - Abstract
Objective To explore the clinical and pathologic features of ovarian juvenile granulosa cell tumors (JGCTs). Methods Clinical data, histopathologic observations, immunohistochemical results, FOXL2 mutation status, and follow-up information of 7 JGCT cases were studied. Results The patients most commonly presented with abdominal distension and pain (5 cases), followed by precocious puberty (1 case) and a pelvic mass (1 case). Six patients had stage I disease, and 1 had stage IV disease. The microscopic examinations typically showed lobular growth punctuated by variably sized and shaped follicles. Rare features included a reticular-cystic appearance mimicking a yolk sac tumor (2 cases), a lobular appearance similar to a sclerosing stromal tumor (1 case), strands and cords (1 case), pseudopapillary appearance (2 cases), spindle cell appearance (1 case), microcystic appearance (1 case), hobnail cells (1 case), and rhabdomyoid cells (1 case). No FOXL2 mutation was encountered. After a median follow-up of 53 months, only 1 patient with a strongly diffuse TP53-positive tumor died of the disease, and 2 successfully had babies. Conclusions JGCT is a rare neoplasm with a wide morphologic spectrum and is easily confused with other tumors. Familiarity with the characteristics, rare atypical appearances, and immunohistochemical results may aid in obtaining a correct diagnosis.
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- 2020
17. Utilization of longitudinal ultrasound measurements to quantify changes in knee joint synovial vascularity in a rabbit model of rheumatoid arthritis
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Shuqiang Chen, Qing Zheng, Yuhong Ye, Hui Liu, Zhen Ye, Chao Huang, and Guorong Lyu
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Vascularity ,business.industry ,Rheumatoid arthritis ,Ultrasound ,Rabbit model ,Medicine ,medicine.symptom ,Knee Joint ,business ,Nuclear medicine ,medicine.disease - Abstract
Objective: This study of rabbits with ovalbumin-induced arthritis (OIA), a model of rheumatoid arthritis (RA), examined the time course of changes in synovial neovascularization based on imaging from power Doppler ultrasound (PDUS) and contrast-enhanced ultrasound (CEUS).Methods: 25 male New Zealand rabbits were in the OIA group and 5 were in the control group. Both rear knee joints of all rabbits were examined using conventional US and CEUS over 16 weeks. The knee synovia of OIA rabbits were sampled by US-guided biopsy, and the expression of CD31 and VEGF were determined by immunohistochemistry. The correlation of microvessel density (CD31 positivity) and VEGF at different times was analyzed using multimodal US.Results: OIA rabbits had increased synovial expression of CD31 and VEGF from week 6 to 12 (PPPConclusions: OIA rabbits mimicked early-stage RA at 4 to 8 weeks, middle-stage RA at 8 to 12 weeks, and late-stage RA at 12 to 16 weeks. PDI, CEUS, and PI, especially when combined with CD31 expression, accurately characterized the extent of synovial vascularization. Increased vascular morphology based on CEUS may have value for the early diagnosis of RA.
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- 2020
18. Value of contrast-enhanced ultrasound for detection of synovial vascularity in experimental rheumatoid arthritis: an exploratory study
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Guorong Lv, Shuqiang Chen, Zhen Ye, Qing Zheng, Hui Liu, Yuhong Ye, and Chao Huang
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Male ,musculoskeletal diseases ,medicine.medical_specialty ,Medicine (General) ,Knee Joint ,Biopsy ,Contrast Media ,Biochemistry ,Arthritis, Rheumatoid ,angiogenesis ,03 medical and health sciences ,0302 clinical medicine ,Vascularity ,R5-920 ,antigen-induced arthritis ,microvessel density ,Synovitis ,medicine ,Animals ,Rheumatoid arthritis ,Ultrasonography ,030203 arthritis & rheumatology ,business.industry ,Synovial Membrane ,Biochemistry (medical) ,Ultrasound ,Cell Biology ,General Medicine ,Hypervascularity ,Pre-Clinical Research Reports ,PDUS ,medicine.disease ,Antigen induced arthritis ,030220 oncology & carcinogenesis ,Microvessels ,Rabbit model ,cardiovascular system ,CEUS ,Rabbits ,Radiology ,medicine.symptom ,business ,Contrast-enhanced ultrasound - Abstract
Objective This study aimed to examine the associations between contrast-enhanced ultrasound (CEUS) imaging and synovial hypervascularity and synovitis score in a rabbit model of antigen-induced arthritis (AIA), compared with power Doppler ultrasound (PDUS). Methods We investigated 50 knee joints in 25 AIA rabbits (AIA group), and 10 knee joints in five sham-injected rabbits (control group). PDUS and CEUS images were evaluated at the 8th week. Ultrasound-guided synovial biopsies were targeted in the area with hypervascularity, and synovial microvessel density (MVD) was evaluated by immunohistochemical staining of CD31. Results The PDUS score was significantly higher in the AIA group (2.61 ± 0.78) compared with the control group (0.50 ± 0.53). CEUS in the AIA group revealed a fast-in/slow-out pattern of contrast enhancement. MVD revealed by CD31+ vessel count and the synovitis score were significantly higher in the AIA group compared with the control group. In the AIA group, CEUS findings showed a better correlation with MVD revealed by CD31+ and synovitis score than PDUS findings. Conclusion CEUS is superior to PDUS for estimating synovial hypervascularity and hyperplasia in experimental rheumatoid arthritis.
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- 2019
19. High ALDH1A1 expression indicates a poor prognosis in gastric neuroendocrine carcinoma
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Sheng Zhang, Yuhong Ye, Yupeng Chen, Pengcheng Wang, and Xingfu Wang
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Adult ,Male ,0301 basic medicine ,Oncology ,medicine.medical_specialty ,Multivariate analysis ,Lymphovascular invasion ,Aldehyde Dehydrogenase 1 Family ,Pathology and Forensic Medicine ,03 medical and health sciences ,0302 clinical medicine ,Stomach Neoplasms ,Cancer stem cell ,Cell Line, Tumor ,Internal medicine ,Biomarkers, Tumor ,medicine ,Humans ,Lymph node ,Survival analysis ,Aged ,biology ,business.industry ,Gastric Neuroendocrine Carcinoma ,Retinal Dehydrogenase ,Cell Biology ,Aldehyde Dehydrogenase ,Middle Aged ,Prognosis ,Carcinoma, Neuroendocrine ,Gene Expression Regulation, Neoplastic ,ALDH1A1 ,030104 developmental biology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,biology.protein ,Immunohistochemistry ,Female ,business - Abstract
Objective To evaluate the expression and prognostic significance of ALDH1A1 in gastric neuroendocrine carcinoma. Materials and methods Immunohistochemical stains of ALDH1A1 were evaluated in 67 cases of gastric neuroendocrine carcinoma. The findings were correlated with clinicopathologic variables and overall survival. Results Immunohistochemistry revealed positive cytoplasmic immunoreactivity in 35 of 67 (52.2%) tumors and strongly positive immunoreactivity in 14 of 67 (20.9%). Strongly positive ALDH1A1 expression, but not positive staining, was significantly associated with lymph node status, lymphovascular invasion, and ki-67 index (P = 0.039, 0.045, and 0.045, respectively). Kaplan‐Meier survival curves and log-rank tests showed significantly poorer prognoses in cases of high ALDH1A1 expression compared to cases of low ALDH1A1 expression or the negative control group (MST, 17 vs. 52 months; P = 0.026). Multivariate analysis showed that high ALDH1A1 expression, lymph node metastasis, and lymphovascular invasion had significant associations with decreased overall survival (P = 0.029, 0.008, and 0.005, respectively). Conclusions High ALDH1A1 expression may be a prognostic indicator of survival in patients with gastric neuroendocrine carcinoma.
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- 2018
20. Protein phosphatase 2A modulates podocyte maturation and glomerular functional integrity in mice
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Jianhua Mao, Chengxian Xu, Yingying Zhang, Weiqiang Lin, Xiujuan Zhu, Yuhong Ye, Jing Zhou, and Cunji Gao
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Male ,Phosphatase ,Kidney Glomerulus ,lcsh:Medicine ,Podocyte ,macromolecular substances ,Biochemistry ,environment and public health ,YB-1 ,Gene Expression Regulation, Enzymologic ,Dephosphorylation ,Gene Knockout Techniques ,Mice ,Western blot ,medicine ,Glomerulopathy ,Animals ,Humans ,Protein phosphorylation ,Protein Phosphatase 2 ,lcsh:QH573-671 ,Phosphorylation ,Molecular Biology ,Cytoskeleton ,medicine.diagnostic_test ,Chemistry ,lcsh:Cytology ,Podocytes ,Research ,lcsh:R ,Body Weight ,Cell Biology ,Protein phosphatase 2 ,Cell biology ,PP2A ,enzymes and coenzymes (carbohydrates) ,Proteinuria ,medicine.anatomical_structure ,embryonic structures ,Slit diaphragm ,Female ,Y-Box-Binding Protein 1 - Abstract
Background Protein phosphorylation & dephosphorylation are ubiquitous cellular processes that allow for the nuanced and reversible regulation of protein activity. Protein phosphatase 2A (PP2A) is a multifunction phosphatase that is well expressed in all cell types of kidney during early renal development, though its functions in kidney remains to be elucidated. Methods PP2A conditional knock-out mice was generated with PP2A fl/fl mice that were crossed with Podocin-Cre mice. The phenotype of Pod-PP2A–KO mice (homozygous for the floxed PP2A allele with Podocin-Cre) and littermate PP2A fl/fl controls (homozygous for the PP2A allele but lacking Podocin-Cre) were further studied. Primary podocytes isolated from the Pod-PP2A-KO mice were cultured and they were then employed with sing label-free nano-LC − MS/MS technology on a Q-exactive followed by SIEVE processing to identify possible target molecular entities for the dephosphorylation effect of PP2A, in which Western blot and immunofluorescent staining were used to analyze further. Results Pod-PP2A–KO mice were developed with weight loss, growth retardation, proteinuria, glomerulopathy and foot process effacement, together with reduced expression of some slit diaphragm molecules and cytoskeleton rearrangement of podocytes. Y box protein 1 (YB-1) was identified to be the target molecule for dephosphorylation effect of PP2A. Furthermore, YB-1 phosphorylation was up-regulated in the Pod-PP2A–KO mice in contrast to the wild type controls, while total and un-phosphorylated YB-1 both was moderately down-regulated in podocytes from the Pod-PP2A-KO mice. Conclusion Our study revealed the important role of PP2A in regulating the development of foot processes and fully differentiated podocytes whereas fine-tuning of YB-1 via a post-translational modification by PP2A regulating its activity might be crucial for the functional integrity of podocytes and glomerular filtration barrier. Graphic abstract Electronic supplementary material The online version of this article (10.1186/s12964-019-0402-y) contains supplementary material, which is available to authorized users.
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- 2019
21. Additional file 1: of Protein phosphatase 2A modulates podocyte maturation and glomerular functional integrity in mice
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Xiujuan Zhu, Yuhong Ye, Chengxian Xu, Cunji Gao, Yingying Zhang, Zhou, Jing, Weiqiang Lin, and Jianhua Mao
- Abstract
Figure S1. Representative image of podocytes seeded on plates coated with collagen type I and laminin from PP2A-KO mice, monitored by live cell imaging for 30, 60 and 120 minutes. No obvious differences in cell spreading were observed from PP2Aâ KO mice when compared with control. (DOCX 570 kb)
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- 2019
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22. Entanglement generation and protection for two atoms in the presence of two parallel mirrors
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Hongwei Yu, Yuhong Ye, and Jiawei Hu
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Physics ,Steady state ,Physics and Astronomy (miscellaneous) ,010308 nuclear & particles physics ,Scalar (mathematics) ,Quantum Physics ,Free space ,Quantum entanglement ,01 natural sciences ,Separable space ,Wavelength ,Quantum mechanics ,0103 physical sciences ,Thermal ,010306 general physics ,Quantum - Abstract
We study, in the framework of open quantum systems, the entanglement generation of two atoms in between two parallel mirrors in a thermal bath of quantum scalar fields. We find that the presence of mirrors plays an important role in entanglement generation and protection. The entanglement dynamics is crucially dependent on the geometric configurations of the two-atom system with respect to the mirrors, and the ranges of temperature and interatomic separation within which entanglement can be generated are significantly changed compared with those in a free space. In particular, when the atomic transition wavelength is larger than twice the distance between the two mirrors, the atoms behave as if they were isolated from the environment and the entanglement can persist in the steady state if the atoms are initially entangled and no entanglement can be created if they are initially separable, no matter how the atoms are placed with respect to the mirrors and to each other. This is in sharp contrast to the fact that in a free space, steady-state entanglement is possible only when the two atoms are placed extremely close to each other, while in the presence of one mirror, it is possible when the two atoms placed extremely close to the mirror.
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- 2021
23. Boosting Both Electrocatalytic Activity and Durability of Metal Aerogels via Intrinsic Hierarchical Porosity and Continuous Conductive Network Backbone Preservation
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Yujing Sun, Jing Zhou, Xubing Lu, Amare Aregahegn Dubale, Yi Li, Yuhong Ye, Dongyang Lou, Zhikun Zheng, Navpreet K. Sethi, Zhao Jin, Jing Yang, Wei Liu, Yuanyuan Zheng, and Honglei Wang
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Metal ,Backbone network ,Materials science ,Renewable Energy, Sustainability and the Environment ,visual_art ,visual_art.visual_art_medium ,Fuel cells ,General Materials Science ,Nanotechnology ,Porosity ,Durability ,Electrical conductor - Published
- 2020
24. Ependymoma with cartilaginous metaplasia might have more aggressive behavior: a case report and literature review
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Xingfu Wang, Xueyong Liu, Yupeng Chen, Sheng Zhang, and Yuhong Ye
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Ependymoma ,Male ,Cancer Research ,Pathology ,medicine.medical_specialty ,medicine.medical_treatment ,Biology ,Fatal Outcome ,Metaplasia ,medicine ,Humans ,Fourth Ventricle ,Glial fibrillary acidic protein ,Brain Neoplasms ,Cartilage ,Cartilaginous metaplasia ,General Medicine ,medicine.disease ,Radiation therapy ,medicine.anatomical_structure ,Oncology ,Child, Preschool ,biology.protein ,Neurology (clinical) ,Osseous metaplasia ,Epithelial Membrane Antigen ,medicine.symptom - Abstract
Ependymoma with cartilaginous metaplasia with or without bone formation is exceedingly rare. Only eight cases have been reported in the literature. We report a case of ependymoma with cartilaginous and osseous metaplasia in a 5-year-old boy. Microscopically, the tumor was composed of neoplastic ependymal tissue and mature cartilage and bone. Immunohistochemically, glial fibrillary acidic protein and epithelial membrane antigen were positive for ependymoma cells but negative for cartilage and bone. Recurrence occurred after 15-month follow-up. The patient deteriorated rapidly and died after 1 month. Reviewing 8 reported cases and our latest case, we found that 3 cases of ependymoma with cartilaginous metaplasia were treated with radiotherapy. Six cases had recurrence from 6 months to 8 years and 2 cases died on the day of operation. These findings suggest that ependymoma with cartilaginous metaplasia might have more aggressive clinical behavior.
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- 2011
25. Histological characterisation and prognostic evaluation of 62 gastric neuroendocrine carcinomas.
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Deng, Yujie, Xiaohui Chen, Yuhong Ye, Xi Shi, Kunshou Zhu, Liming Huang, Sheng Zhang, Mingang Ying, and Xuede Lin
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NEUROENDOCRINE tumors ,GASTRIC diseases ,SYNAPTOPHYSIN ,CHROMOGRANINS ,HISTOPATHOLOGY ,GENETICS ,PROGNOSIS - Abstract
Aim of the study: To determine the significance of expression of synaptophysin, chromogranin A, and Ki-67 and their association with clinicopathological parameters, and to find out the possible prognostic factors in gastric neuroendocrine carcinoma (G-NEC). Material and methods: We investigated the immunohistochemical features and prognosis of 62 G-NECs, and evaluated the association among expressions of synaptophysin, chromogranin A, and Ki-67, clinicopathological variables, and outcome. Results: Chromogranin A expression was found more commonly in smallcell NECs (9/9, 100%) than in largecell NECs (27/53, 51%) (p = 0.008). No statistical significance was found in Ki-67 (p = 0.494) or synaptophysin (p > 0.1) expression between NEC cell types. Correlation analyses revealed that Ki-67 expression was significantly associated with mid-third disease of stomach (p = 0.005) and vascular involvement (p = 0.006), and had a trend of significant correlation with tumour relapse (p = 0.078). High expression of chromogranin A was significantly associated with histology of small-cell NECs (p = 0.008) and lesser tumour greatest dimension (p = 0.038). The prognostic significance was determined by means of Kaplan-Meier survival estimates and log-rank tests, and as a result, early TNM staging and postoperative chemotherapy were found to be correlated with longer overall survival (p < 0.05). Univariate analysis revealed associations between poor prognosis in NECs and several factors, including high TNM staging (p = 0.048), vascular involvement (p = 0.023), relapse (p = 0.004), and microscopic/macroscopic residual tumour (R1/2, p < 0.001). In a multivariate analysis, relapse was identified as the sole independent prognostic factor. Conclusions: No significant correlation between survival and expression of synaptophysin, chromogranin A, or Ki-67 has been determined in G-NECs. Our study indicated that early diagnosis, no-residual-tumour resection, and postoperative chemotherapy were possible prognostic factors. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
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