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Landslides induced by reservoir inundation are common in Southwest China, negatively influencing hydropower stations. The Wunonglong hydropower station dam was constructed in the upper reaches of the Lancang River, accordingly causing the water level at the Lajinshengu slope to increase by 30 m. A tension crack with a visible depth of 8 m was observed in the upper sector of the Lajinshengu slope after reservoir impoundment for 170 d. In the following days, numerous cracks appeared on the surface of the slope, and the maximum displacement of the slope reached 3.22 m. Then, a large-scale active deformation body within the Lajinshengu slope formed with an area of 2.62 × 105 m2 and a volume of 1.65 × 107 m3. Detailed field investigations, on-site monitoring, and centrifugal model tests were carried out to analyze the surface features, deformation characteristics, and failure mechanism of the Lajinshengu slope. The results show that the slope is an ancient landslide, divided into two parts (i.e. zone A and zone B) by the gully. Zone B is a traction landslide caused by the displacement of zone A. The long-term inundation weakens the soft rock at the slope foot, intensifying the toppling of bedrock and consequently triggering the sliding of the overburden in zone A. The failure mode of the Lajinshengu slope is a typical case of toppling-sliding failure, and the underlying rock toppling drives the overlying sliding. In addition, early identification methods for toppling deformation covered by overburdened soil were proposed based on monitoring data and deformation signs.

IntroductionInvasive species can cause ecological and economic damage in various areas, including nature reserves. The invasion risks of aquatic invasive vertebrates in nature reserves, however, remain unclear since this group often hides under the water and is frequently neglected in ecological surveys based on traditional methods.MethodsEnvironmental DNA (eDNA) provides a promising alternative way to conduct biodiversity surveys in aquatic ecosystems. Here, we collected aquatic eDNA samples from eight nature reserves in Guangdong Province, China to mainly investigate the diversity of aquatic invasive vertebrates and inform their invasion risks in these nature reserves.Results and discussionWe detected a total of 104 aquatic vertebrate species belonging to three classes (Actinopteri, Amphibia and Reptilia), 12 orders, 32 families, and 71 genera, among which nine were invasive species (8.65% of all aquatic vertebrates detected), i.e., Coptodon zillii, Sarotherodon galilaeus, Oreochromis niloticus, Oreochromis tanganicae, Gambusia affinis, Clarias gariepinus, Chelydra serpentina, Trachemys scripta elegans, and Rana catesbeiana. Surprisingly, 55.56% of these aquatic invasive vertebrates (i.e., five species) were found in at least 75.00% samples, and both C. zillii and S. galilaeus were detected in all samples (100%), suggesting that most invasive species were widely distributed in these nature reserves. In addition, all aquatic invasive vertebrate species ranked very high (top 66 of aquatic vertebrates detected) regarding their relative abundance of sequences, and three of the top 10 species with the highest number of sequences were invasive species (i.e., C. zillii, S. galilaeus, and O. niloticus), suggesting high population size of these invasive vertebrates. Moreover, we also detected 16 endangered/threatened species (15.38% of all vertebrates detected), which demonstrated notable overlaps of geographic distribution with invasive species. The reality of high abundance, wide geographical distribution and overlaps with the endangered/threatened species indicated considerable risks of aquatic invasive vertebrates in nature reserves in Guangdong Province, which calls for urgent needs for effective management. Our study would provide fundamental insights for the formulation of effective management measures to reduce losses caused by invasive species and promote the protection of endangered/threatened species in nature reserves.

Primary toppling usually occurs in layered rock slopes with large anti-dip angles. In this paper, the block toppling evolution was explored using a large-scale centrifuge system. Each block column in the layered model slope was made of cement mortar. Some artificial cracks perpendicular to the block column were prefabricated. Strain gages, displacement gages, and high-speed camera measurements were employed to monitor the deformation and failure processes of the model slope. The centrifuge test results show that the block toppling evolution can be divided into seven stages, i.e. layer compression, formation of major tensile crack, reverse bending of the block column, closure of major tensile crack, strong bending of the block column, formation of failure zone, and complete failure. Block toppling is characterized by sudden large deformation and occurs in stages. The wedge-shaped cracks in the model incline towards the slope. Experimental observations show that block toppling is mainly caused by bending failure rather than by shear failure. The tensile strength also plays a key factor in the evolution of block toppling. The simulation results from discrete element method (DEM) is in line with the testing results. Tensile stress exists at the backside of rock column during toppling deformation. Stress concentration results in the fragmented rock column and its degree is the most significant at the slope toe.

Abstract With wave-particle decomposition, a unified gas-kinetic wave-particle (UGKWP) method has been developed for multiscale flow simulations. With the variation of the cell Knudsen number, the UGKWP method captures the transport process in all flow regimes without the kinetic solver’s constraint on the numerical mesh size and time step being determined by the kinetic particle mean free path and particle collision time. In the current UGKWP method, the cell Knudsen number, which is defined as the ratio of particle collision time to numerical time step, is used to distribute the components in the wave-particle decomposition. The adaptation of particles in the UGKWP method is mainly for the capturing of the non-equilibrium transport. In this aspect, the cell Knudsen number alone is not enough to identify the non-equilibrium state. For example, in the equilibrium flow regime with a Maxwellian distribution function, even at a large cell Knudsen number, the flow evolution can be still modelled by the Navier-Stokes solver. More specifically, in the near space environment both the hypersonic flow around a space vehicle and the plume flow from a satellite nozzle will encounter a far field rarefied equilibrium flow in a large computational domain. In the background dilute equilibrium region, the large particle collision time and a uniform small numerical time step can result in a large local cell Knudsen number and make the UGKWP method track a huge number of particles for the far field background flow in the original approach. But, in this region the analytical wave representation can be legitimately used in the UGKWP method to capture the nearly equilibrium flow evolution. Therefore, to further improve the efficiency of the UGKWP method for multiscale flow simulations, an adaptive UGKWP (AUGKWP) method is developed with the introduction of an additional local flow variable gradient-dependent Knudsen number. As a result, the wave-particle decomposition in the UGKWP method is determined by both the cell and gradient Knudsen numbers, and the use of particles in the UGKWP method is solely to capture the non-equilibrium flow transport. The current AUGKWP method becomes much more efficient than the previous one with the cell Knudsen number only in the determination of wave-particle composition. Many numerical tests, including Sod shock tube, normal shock structure, hypersonic flow around cylinder, flow around reentry capsule, and an unsteady nozzle plume flow, have been conducted to validate the accuracy and efficiency of the AUGKWP method. Compared with the original UGKWP method, the AUGKWP method achieves the same accuracy, but has advantages in memory reduction and computational efficiency in the simulation for flows with the co-existing of multiple regimes.

Introduction: Obesity has previously been correlated with an elevated risk of reproductive system diseases in women. The waist-hip ratio (WHR) has been shown to be correlated with visceral fat, making it one of the most commonly used indicators of abdominal obesity. However, little is known about the relationship between WHR and infertility. Therefore, the aim of this study was to evaluate the effect of the WHR on infertility in women of childbearing age. Methods: The study used cross-sectional data from women aged 20 to 45 who participated in the National Health and Nutrition Examination Survey (NHANES), which was conducted between 2017 and 2020. We collected details of their Waist Circumference, hip Circumference, fertility status and several other essential variables. We used multivariate logistic regression analysis and subgroup analyses to assess the association between WHR and infertility. Results: There were 976 participants, with 12.0% (117/976) who experienced infertility. After adjusting for potential confounding factors, our multivariate logistic regression analysis revealed that every 0.1 unit increase in WHR resulted in a more than 35% higher risk of infertility (OR (95% CI): 1.35 (1.01~1.81), P = 0.043). Compared to the group with WHR

Species distribution models (SDMs) have been widely used to predict potentially suitable habitats for invasive alien species (IAS) and evaluate invasion risk. However, SDMs have been discredited because they ignore the physiological processes by which species respond to their environment. Integrating physiological tolerance into the model is essential to improve the prediction accuracy of SDMs. Currently, this approach has not been applied in the study of the worldwide invasive species, the red-eared slider (Trachemys scripta elegans), which is one of the world’s 100 worst invasive species and is widespread in China. In this study, based on hatching experiments, we found that the embryo temperature tolerance range of the red-eared slider was between 21.8 °C and 33.1 °C. Further, we studied the effect of embryo temperature tolerance on the prediction of potential invasion areas for this alien reptile species. The high suitability area (530,214.71 km2) predicted by the SDM incorporating embryo temperature tolerance data were 20.9% smaller than that (641,107.60 km2) predicted by the SDM without considering embryo temperature tolerance. The difference between the two SDMs is primarily concentrated at the edges of the high suitability areas. The incorporation of embryo temperature tolerance data influenced the model's predictions by effectively reducing the extent of edges of the high suitability areas. High suitability areas for red-eared sliders are mainly concentrated in South China, Central China, and East China, with a few in North and Southwest China. There is almost no invasion risk in most of the northeast and northwest provinces of China. This study not only has theoretical significance for optimizing model predictions, but also provides an important scientific basis for prevention and risk assessment of invasion by red-eared sliders in China.

The fracture extension of rock bridges between structural surfaces in different parts of the slope leads to the damage of the anti-dipping laminated rock slope. In order to study the controlling effect of fracture extension of non-penetrating fractures in the slope on the evolution of the slope, the centrifugal model test of the anti-dipping laminated rock slope containing multiple groups of non-penetrating fractures is carried out with the right shoulder of the Miaowei Hydropower Station as the geological prototype to analyze the deformation characteristics of non-penetrating fractures in the anti-dipping laminated rock slope. The results show that the fractures of rock formations containing non-penetrating fractures within the slope finally show five types of fracture patterns, including inter-fracture bridge penetration, penetration between gently dipping fractures and the upper rock layer, penetration between steeply dipping fractures and the lower rock layer, penetration between steeply dipping fractures and the port of gently dipping fractures, and fracture of rock layers at non-fractures, with inter-fracture bridge penetration as the main fracture pattern. Based on fracture mechanics and combined with the principle of fracture superposition, it is found that the instability factor of the rock layer at the main fracture section is the smallest at 1/3 of the slope height and gradually becomes larger towards the foot and both sides of the slope top, while the stress intensity factor gradually decreases from 1/3 of the slope height to the foot and the top of the slope. The fracture extension of cracks controls the slope evolution and is greatly influenced by the crack rate and the tip stress field around the cracks. At the early stage of slope evolution, the compression and settlement of the back edge of the slope and the compression and shear damage of the local rock cracks are the main features, and the dip angle of the rock layers changes greatly, showing a trend of becoming larger from the upper part of the slope to the lower part of the slope. At the middle of the evolution, the fracture extension of the back edge of the slope forms the main fracture surface, and the angle of the rock layers in the middle and upper part of the slope changes greatly. At the end of the evolution, the number of fractures remains stable, and the main deformation feature is characterized by the redistribution of the location of fractured rock layers, the formation of secondary fracture surfaces, further compression between broken rock layers, and further destabilization of the slope body.

Summary: The critically endangered big-headed turtle (Platysternon megacephalum) is currently classified into three subspecies. However, the classification is still controversial and their evolutionary histories are still unclear. Here, multiple genetic analyses consistently revealed three phylogenetic groups with substantial genetic divergences and distinct demographic histories, suggesting three phylogenetic species (P. megacephalum, P. peguense, and Baise clade). Phylogeographical analyses revealed that the Red River plains and Guangxi basins are largely coincident with the boundaries between the three phylogenetic species, highlighting the key role of lowland areas in driving speciation in the big-headed turtle. The Baise clade is characterized by high-linkage disequilibrium but the lowest effective population size, indicating that the cryptic phylogenetic species is more vulnerable to human activities and environmental disturbance, and urgently needs more protection. Our findings provide fundamental insights into the taxonomy and scientific conservation of the family Platysternidae.

Abstract The big‐headed turtle (Platysternon megacephalum) is an endemic chelonian species in Asia. Unlike most other turtles in the world, P. megacephalum is characterized with eagle‐beak jaw, large head, and long tail. Although these unique characteristics are well recognized, the underlying genetic basis remains largely elusive. Here, we performed comparative genomic analysis between P. megacephalum and other representative species, aiming to reveal the genetic basis of the unique morphological features. Our results revealed that the eagle‐beak jaw is most likely enabled by combined effects of expansion of SFRP5, extraction of FGF11, and mutation of both ZFYVE16 and PAX6. Large head is supported by mutations of SETD2 and FGRF2 and copy number variations of six head circumference modulation‐related genes (TGFBR2, Twist2, Rdh10, Gas1, Chst11, and SNAP25). The long tail is probably involved in a genetic network comprising Gdf11, Lin 28, and HoxC12, two of which showed a consistent expression pattern with a model organism (mice). These findings suggest that expansion, extraction, and mutation of those genes may have profound effects on unique phenotypes of P. megacephalum.

The yellow pond turtle (Mauremys mutica) is widely cultured for food, medicine and for keeping as pets in China. The high risk of disease outbreaks and mortality caused by sudden sharp temperature drop events has significant negative effects on the aquaculture industry of M. mutica. However, the mechanism underlying the damage caused by cold stress is still unclear. To fill this gap, we performed transcriptome sequencing of M. mutica samples collected at three sampling time points (3h, 24h and 48h) during cold treatment, and at a recovery time point (96 h after the end of cold stress). The results showed that immunity and metabolism-related pathways (neuroactive ligand-receptor interactions, cytokine-cytokine receptor interactions and NOD-like receptor signaling pathways) were significantly enriched at both 24h and 48h. The expression of immunity and metabolism-related genes (BS2A1, PIWIL2 and Fads1) significantly decreased at all-time points compared to the control group. These results suggested that impaired immunity and depressed metabolism under cold stress may be the main cause of the massive cold-stress mortality of M. mutica. This study provides novel insights into the molecular mechanisms underlying mass disease and mortality caused by sudden sharp temperature drop events in M. mutica.

Both the undisturbed soil and remolded soil have initial structural properties, and the shear stress resisted by the initial structure of the soil is the structural strength of the soil. In order to study the initial structural strength of the uncemented coarse-grained soil, this paper defines the characteristics of the initial structural change points of the coarse-grained soil during the shearing process, and divides the coarse-grained soil into four structural types according to the content of the coarse-grained soils greater than 20 mm. Visual direct shear instrument direct shear tests are carried out on the coarse-grained soils with different structural characteristics, and the critical strength of the coarse-grained soils with different initial structures is determined through the numerical value and the change trend of the initial structure indexes of the coarse-grained soils during the shearing process. The results show that the shear strength of the coarse-grained soils with different initial structure types under the same normal stress and the same particle type are in the same order as the proportion of the structural strength, indicating that the particle structure strength plays a role in the whole process of the resisting shear stress. The average structural strength of the four initial structure types of the coarse-grained soils accountes for 36.27%, that is, the structural strength accountes for 36.27% of the peak shear strength. The arranged contact structure has the highest structural strength at 36.62%, followed by the mosaic structure 36.61%. The suspended dense structure is 35.99%, and the superimposed structure has the lowest proportion of the structural strength, which is 35.87%.

This study focuses on optimizing the foundation pit dewatering scheme using the foundation pit dewatering theory and the principles of multi-objective optimization. It explores the development of a multi-objective optimization model and efficient solution technology for foundation pit dewatering. This research focuses on the foundation pit dewatering project at the inverted siphon section of Xixiayuan canal head, specifically from pile number XZ0+326 to XZ0+500. It establishes an optimized mathematical model for foundation pit dewatering that incorporates three objectives. Additionally, a dewatering optimization program is developed by utilizing the MATLAB optimization toolbox and the multi-objective optimization algorithm program based on the NSGA-II algorithm (Gamultiobj). The multi-objective optimization mathematical model is solved, and a Pareto-optimal solution set with uniform distribution is obtained. The multi-objective optimization evaluation system based on AHP is constructed from the three aspects of dewatering cost, the impact of settlement on the environment, and the safety and stability of the foundation pit. The optimization scheme of the Pareto-optimal solution set is selected as the decision result to provide multiple feasible schemes for the dewatering construction of foundation pits. The optimization scheme is verified by using the GMS software. The simulation results demonstrate that the optimization scheme fulfills the requirements for water level and settlement control. Moreover, the developed optimization program efficiently solves the multi-objective optimization problem associated with foundation pit dewatering. Lastly, an evaluation system incorporating the NSGA-II algorithm and AHP is developed and utilized in the context of dewatering engineering in order to offer multiple viable optimal dewatering schemes.

Abstract Phosphoprotein enriched in astrocytes, 15 kDa (PEA-15) is a death-effector domain (DED) containing protein involved in regulating mitogen-activated protein kinase and apoptosis pathways. In this molecular dynamics study, we examined how phosphorylation of the PEA-15 C-terminal tail residues, Ser-104 and Ser-116, allosterically mediates conformational changes of the DED and alters the binding specificity from extracellular-regulated kinase (ERK) to Fas-associated death domain (FADD) protein. We delineated that the binding interfaces between the unphosphorylated PEA-15 and ERK2 and between the doubly phosphorylated PEA-15 and FADD are similarly composed of a scaffold that includes both the DED and the C-terminal tail residues of PEA-15. While the unphosphorylated serine residues do not directly interact with ERK2, the phosphorylated Ser-116 engages in strong electrostatic interactions with arginine residues on FADD DED. Upon PEA-15 binding, FADD repositions its death domain (DD) relative to the DED, an essential conformational change to allow the death-inducing signaling complex (DISC) assembly.

An obese mouse model induced by high-fat diet (HFD) feeding was used to reveal the role of piperine in modulating gut microbiota (GM). Piperine was administrated at 20 and 40 mg/kg body weight every day. As a result, piperine at 40 mg/kg significantly decreased body weight, liver weight, perirenal fat weight, and lowered serum triglycerides, total cholesterol, low-density lipoprotein cholesterol, and glucose levels in HFD-fed mice. Additionally, piperine significantly attenuated fatty liver and modulated hepatic mRNA expressions of SREBP-1c, SREBP2, and HMGCR. In perirenal fat, FAS, C/EBPα, MCP1, and IL-6 expressions were significantly downregulated by piperine. 16S rRNA sequencing revealed that piperine elevated GM diversity. The relative abundance of Muribaculaceae and Ruminococcaceae were significantly elevated, while Dubosiella and Enterorhabdus genera were suppressed by piperine. The Pearson correlation analysis showed that the altered phylotypes were highly correlated with obesity phenotypes. These findings suggest that piperine modulates energy homeostasis and inflammation to alleviate obesity associated with GM regulation.

Particle shape is one of the main factors that affect the compactness, mechanics and seepage of coarse-grained soil. In order to analyze the influence of particle shape on the shear characteristics of coarse-grained soil, the discrete element method is used to generate four different particle groups. The direct shear test simulation and macro-micro shear response research of coarse-grained soil are carried out. The influence of particle shape on shear stress-shear displacement and volume strain-shear displacement is obtained. The shear stress, strain characteristics and dilatancy characteristics of coarse-grained soil are analyzed. The influence of particle shape on coarse-grained soil on macro-micro scale is studied by analyzing the evolution law of macro-micro parameters such as shear zone thickness, particle rotation value, average contact number, porosity and contact force system. The results show that the self-locking effect of the special-shaped particles is greater than that of the pure round particles, and the shear strength of the sample tends to increase with the decreasing shape coefficient. The sample particles move under the action of external load, and the strain is mainly shown in the shear zone where the particles move violently and dilate greatly. The particle shape coefficient decreases, the initial average contact number of the sample increases, the internal friction angle "φ" increases, and the larger the increment of porosity in the shear band, the larger the dilatancy amplitude. During the shearing process, the strong chain gathers in the shearing zone and acts as a skeleton. With the decrease in the shape coefficient, the percentage of the force chain length in the range of 0 - 5 increases. The number of strong chains in the shear band increases with the decrease in the shape coefficient, and the peak value content ranges between 30% and 35%.

This study conducts a field indoor simulation test, SEM observation, and penetration test to determine the bearing capacity of the dynamic driving pile in the mudstone foundation. It comprehensively analyzes the variation laws of structure and strength of mudstone around piles after piling. Indeed, the strength of mudstone structure is significantly reduced from outside to inside. Therefore, the numerical simulation of piles in mudstone should consider the actual characteristics of soil damage around piles. The strength of mudstone after pile driving damage is measured, and the scatter diagram depicting the relationship between mudstone strength and pile side distance is produced. Then, the best-fitting curve of the relationship between the strength ratio and the distance ratio of the simulated pile driving test is established by the nonlinear fitting of multiple curves. A numerical simulation method is proposed to consider the damaged area and parameters surrounding the pile. The range of soil damage caused by pile driving in the mudstone foundation is determined to be two times that of the pile diameter. The disturbance area is divided into four parts on average, and the width of each part is 0.5d. The simulation results are compared to the conventional approach of uniform parameter assignment to prove the rationality of the method.

In large-scale water diversion projects, especially in the central and western regions of China, long-distance deep buried tunnels are generally involved. Therefore, it is essential to carry out field tests to obtain the permeability of the rock mass through which tunnels pass. However, the test holes of large-scale water diversion projects are basically located in mountain areas with complex hydrogeological conditions. Meanwhile, the test holes are far apart and large in depth. As a result, traditional pumping tests cannot meet the requirements. Therefore, the slug test was chosen as the main test method, and the calculation results of the water injection test, the water pressure test and the slug test are analyzed and compared. The calculation results of the three test methods are basically consistent. However, the water injection test and the water pressure test are difficult to implement at a large scale due to many environmental constraints, complex test equipment, long test periods and other factors. Furthermore, the Kipp model, the CBP model and the proposed HWS model, considering the effect of the finite thickness well-skin layer for the first time, were used to analyze and process the slug test data, respectively. The curve fitting effect of the Kipp model was the best, but the calculations were generally larger. The difference between the CBP model and the proposed HWS model is smaller in the calculation results; however, the curve fitting effect of the CBP model is the worst, and the CBP model needs to be further improved. The curve fitting effect of the proposed HWS model was between that of the Kipp model and the CBP model, and the proposed HWS model can be applied to the parameter calculations of the slug test with well-skin. In general, with reference to the criteria for the damping coefficient of the aquifer in the Kipp model, the Kipp model was applicable to the slug test for test holes without well-skin and an aquifer damping coefficient between 0.1 and 5.0. The CBP model was applicable to the slug test under the conditions of no well-skin and an aquifer damping coefficient greater than 2.0. The novel proposed HWS model was applicable to the slug test when the aquifer damping coefficient was greater than 1.0 under the conditions of no well-skin, positive well-skin and negative well-skin.

As important parameters for characterizing heat transfer, thermal property parameters of aquifers and rock-soil skeletons have important research significance in the development and utilization of geothermal resources. The slug heat test is inspired by the slug test, and the heat is instantaneously excited in the test well so as to change the temperature of test section in the test well instantaneously. Based on the thermal radial convection-dispersion theory and the principle of heat conservation, the theoretical model of the slug heat test is established, and the model is solved by Laplace transform and inverse transform to obtain multiple sets of standard curves under different conditions. The slug heat tests were conducted in the indoor model, the slug heat test data under different hydrodynamic conditions were fitted with the standard curves and the thermal property parameters, including effective thermal conductivity, stagnant thermal conductivity, thermal mechanical dispersion coefficient, thermal dispersive degree, thermal diffusivity, heat capacity of aquifer, heat capacity and thermal conductivity of rock-soil skeletons, were accurately obtained. The test results are in good agreement with the empirical values. Meanwhile, the effective thermal conductivity of the aquifer also clearly increases with the increase of flow rate. The excitation temperature difference had little effect on the effective thermal conductivity of the aquifer. At the same time, numerical simulation methods are used to establish a numerical model consistent with the indoor test model, and the numerical model is assigned with the thermal property parameters obtained from the indoor slug heat test, and the measured values of temperature changes in the test well during the slug heat test under different hydrodynamic and excitation strength conditions are compared with the simulated values for verification. The research results show that the slug heat test has the characteristics of high applicability, simple operation and rapid testing, and can effectively determine the thermal properties parameters of aquifers and rock-soil skeletons.

Based on the proposed calculation method for determining the different dip angle fracture permeability coefficients derived from the well flow vibration equation and the self-developed slug test system, research on the slug test in a field in fractured rock mass was carried out based on the underground water sealed cavern project of the National Huangdao Petroleum Reserve. The formation lithology of the test site was granite gneiss, and the fractures were not developed, which is conducive to the research of slug tests on fracture permeability. On the basis of obtaining the geological information of boreholes by using the self-developed slug test system, comparative research on the segmented slug test and conventional water pressure test was carried out. The test results show that the proposed slug test method and self-developed test system has good accuracy and applicability for determining the fracture permeability coefficient, equivalent permeability coefficient and rock mass permeability coefficient tensor, which is more convenient and efficient than other test methods.

Background Reeves’ Turtles (Mauremys reevesii) are economically important in aquaculture in China. Understanding the effects of incubation temperature and substrate moisture on embryos and hatchlings is of great significance for improving the artificial culture of M. reevesii. However, available studies have not yet determined the thermal and hydric optima for M. reevesii eggs, and the potential interaction between the two factors. Methods In this study, eggs of M. reevesii were incubated at five temperature levels (23, 26, 29, 32 and 35 °C, fluctuation range ± 0.5 °C). In each temperature level, there were three substrate moisture levels (1:0.5, 1:0.9 and 1:1.2, weight ratio of vermiculite to water). Thus, a total of 15 combinations of temperature and moisture were used to examine the effects of incubation temperature and substrate moisture on incubation duration, hatching success, hatchling phenotypes, post-hatching growth and hatchling survival. Results Substrate moisture did not significantly affect most development parameters (except incubation duration and carapace width of hatchlings). Eggs incubated at low moisture level (1:0.5) had a longer incubation duration and produced hatchlings with smaller carapace widths than those incubated at medium (1:0.9) or high (1:1.2) moisture levels. Incubation temperature had a significant effect on incubation duration, hatching success, hatchling phenotypes and hatchling survival. Incubation duration decreased as incubation temperature increased. Eggs incubated at 23, 26 and 29 °C showed higher hatching success than those incubated at 32 and 35 °C. Hatchlings incubated at 32 °C were smaller in body size and mass than those incubated at 23, 26 and 29 °C. At 12 months of age, incubation temperature had no long-lasting effect on body mass, but hatchlings incubated at 23 and 35 °C had lower survival rates than those incubated at 26, 29 and 32 °C. For the development of embryos and hatchlings, the interaction between incubation temperature and substrate moisture was not significant. Conclusions Our results indicate that incubation temperature has a significant influence on the development of embryos and hatchlings of M. reevesii, while substrate moisture only significantly affects the incubation duration and carapace width of hatchlings. The combination of an incubation temperature of 29 ± 0.5 °C and a substrate moisture level of 1:1.2 represented optimal incubation conditions in this experiment. Such incubation conditions are helpful in obtaining higher hatching success, shorter incubation duration and higher survival rates for this aquaculture species.

To investigate the effect of drag force on stability of residual soil slopes, a modified approach based on the limit equilibrium method is proposed by establishing a nonlinear coupled mathematical model to calculate the seepage velocities in soils and the runoff velocities at a fluid–solid interface. In this mathematical model, the Navier–Stokes equation and Brinkman-extended Darcy equation are used to describe the incompressible surface runoff and seepage in soils, respectively. Then, the drag force can be derived by adopting Newton's law of internal friction, and the factor of safety (FOS) of residual soil slopes considering drag force can be calculated based on the limit equilibrium method. The modified approach was applied to analyse the stability of rainfall-induced landslides in residual soils in Nanjiang, Sichuan, China, 2011. We found that the runoff depth can significantly control the FOS of the residual soil slopes and cause slope failures. The results of sensitivity analysis highlight the significance for considering drag force due to surface runoff when calculating the FOS of the residual soil slope. The FOS obtained from the modified approach is more reasonable, and this modified approach also can be used for evaluating or predicting rainfall-induced slope instability in residual soils.

We demonstrate a hundred micro-Joules level femtosecond laser system based on a compact and simple two-stage Yb:YAG single crystal fiber chirped pulse amplification system which delivers compressed power of 15.57 W, pulse width of 715 fs. The different amplification performance with different input seed power is experimentally studied. A maximum direct amplified power output of 44 W at 100 kHz is obtained for an input seed power of 12 W. To the best of our knowledge, this is the highest average power of femtosecond laser based on single crystal fiber at hundred micro-Joules energy level.

The carapace color of newborn big-headed turtles (Platysternon megacephalum) is polymorphic and usually consists of two phenotypes: yellowish brown and olive green. As the turtles grew, over the first year of life, its carapace gradually turned from yellowish brown to chestnut brown, or from olive green to dark brown, depending on the phenotype. Meanwhile, the turtle’s plastron remained an orange and black pattern and did not change much. In this study, we primarily used HE staining to observe the carapace color change with age in big-headed turtle juveniles. We took the carapace marginal scute tissues twice from the same turtles before and after the carapace color change. Histological observations show that in the marginal scutes of the four tested turtles with different carapace color phenotypes, melanin granules are all concentrated in the dermal layer underneath the dorsal corneous layer, but rarely on the ventral side. Melanin deposits in the dorsal corneous layer were found to increase as the corneous layers thickened, while the melanin deposits in the ventral corneous layer did not change significantly. However, there was no significant difference in melanin deposition in the epidermis and dermis of the carapace among the yellowish brown, chestnut brown, olive green, and dark brown big-headed turtles. The results of our study indicate that the carapace color darkening in big-headed turtles may not be due to changes in melanin content of the carapace, but is the result of melanin accumulation and superposition in the dorsal corneous layer.

We present as a case study the evolution of a series of participant-centered workshops designed to meet a need in the life sciences education community--the incorporation of best practices in the assessment of student learning. Initially, the ICABL (Inclusive Community for the Assessment of Biochemistry and Molecular Biology/BMB Learning) project arose from a grass-roots effort to develop material for a national exam in biochemistry and molecular biology. ICABL has since evolved into a community of practice in which participants themselves--through extensive peer review and reflection--become integral stakeholders in the workshops. To examine this evolution, this case study begins with a pilot workshop supported by seed funding and thoughtful programmatic assessment, the results of which informed evidence-based changes that, in turn, led to an improved experience for the community. Using participant response data, the case study also reveals critical features for successful workshops, including participant-centered activities and the value of frequent peer review of participants' products. Furthermore, we outline a train-the-trainer model for creating a self-renewing community by bringing new perspectives and voices into an existing core leadership team. This case study, then, offers a blueprint for building a thriving, evolving community of practice that not only serves the needs of individual scientist-educators as they seek to enhance student learning, but also provides a pathway for elevating members to positions of leadership.

As the world endures the coronavirus disease 2019 (COVID-19) pandemic, the conditions of 35 million vulnerable individuals struggling with substance use disorders (SUDs) worldwide have not received sufficient attention for their special health and medical needs. Many of these individuals are complicated by underlying health conditions, such as cardiovascular and lung diseases and undermined immune systems. During the pandemic, access to the healthcare systems and support groups is greatly diminished. Current research on COVID-19 has not addressed the unique challenges facing individuals with SUDs, including the heightened vulnerability and susceptibility to the disease. In this systematic review, we will discuss the pathogenesis and pathology of COVID-19, and highlight potential risk factors and complications to these individuals. We will also provide insights and considerations for COVID-19 treatment and prevention in patients with SUDs.

Phosphoprotein enriched in astrocytes, 15 KDa (PEA-15), a ubiquitously expressed small protein in all mammals, is known for decades for its potent interactions with various protein partners along distinct biological pathways. Most notable interacting partners of PEA-15 include extracellular signal-regulated kinase 1 and 2 (ERK1/2) in the mitogen activated protein kinase (MAPK) pathway, the Fas-associated death domain (FADD) protein involving in the formation of the death-inducing signaling complex (DISC), and the phospholipase D1 (PLD1) affecting the insulin sensitivity. However, the actual cellular functions of PEA-15 are still mysterious, and the question why this protein is expressed in almost all cell and tissue types remains unanswered. Here we synthesize the most recent structural, biological, and clinical studies on PEA-15 with emphases on its anti-apoptotic, anti-proliferative, and anti-inflammative properties, and propose a converged protective role of PEA-15 that maintains the balance of death and survival in different cell types. Under conditions that this delicate balance is unsustainable, PEA-15 may become pathological and lead to various diseases, including cancers and diabetes. Targeting PEA-15 interactions, or the use of PEA-15 protein as therapeutics, may provide a wider window of opportunities to treat these diseases.

Phosphoprotein enriched in astrocytes, 15 kDa (PEA-15) exerts its regulatory roles on several critical cellular pathways through protein−protein interactions depending on its phosphorylation states. It can either inhibit the extracellular signal-regulated kinase (ERK) activities when it is dephosphorylated or block the assembly of death-inducing signaling complex (DISC) and the subsequent activation of apoptotic initiator, caspase-8, when it is phosphorylated. Due to the important roles of PEA-15 in regulating these pathways that lead to opposite cellular outcomes (cell proliferation vs. cell death), we proposed a phosphostasis (phosphorylation homeostasis) model, in which the phosphorylation states of the protein are vigorously controlled and regulated to maintain a delicate balance. The phosphostasis gives rise to the protective cellular functions of PEA-15 to preserve optimum cellular conditions. In this article, using advanced multidimensional nuclear magnetic resonance (NMR) techniques combined with a novel chemical shift (CS)-Rosetta algorithm for de novo protein structural determination, we report a novel conformation of PEA-15 death-effector domain (DED) upon interacting with ERK2. This new conformation is modulated by the irregularly structured C-terminal tail when it first recognizes and binds to ERK2 at the d-peptide recruitment site (DRS) in an allosteric manner, and is facilitated by the rearrangement of the surface electrostatic and hydrogen-bonding interactions on the DED. In this ERK2-bound conformation, three of the six helices (α2, α3, and α4) comprising the DED reorient substantially in comparison to the free-form structure, exposing key residues on the other three helices that directly interact with ERK2 at the DEF-docking site (docking site for ERK, FxF) and the activation loop. Additionally, we provide evidence that the phosphorylation of the C-terminal tail leads to a distinct conformation of DED, allowing efficient interactions with Fas-associated death domain (FADD) protein at the DISC. Our results substantiate the allosteric regulatory roles of the C-terminal tail in modulating DED conformation and facilitating protein−protein interactions of PEA-15.

A laser master oscillator power amplifier (MOPA) system consisting of a fiber amplifier and a two-stage Yb:YAG single crystal fiber (SCF) is experimentally studied. The nonlinear stimulated Raman scattering (SRS) is avoided by limiting the output power of the fiber preamplifier to 600 mW. Due to the benefit from the low nonlinearity and high amplification gain of the SCF, a laser pulse duration of 16.95 ps and a high average power of 41.7 W at a repetition rate of 250 kHz are obtained by using a two-stage polarization controlled double-pass amplification of Yb:YAG SCF, corresponding to an output energy of 166.8 μJ and a peak power of 9.84 MW, respectively. The polarization controlled SCF amplification scheme achieved a gain as high as more than 69 times. During the amplification, the spectra gain narrowing effect and the polarization controlled four-pass amplification setup are also studied. The laser spectrum is narrowed from over 10 nm to less than 3 nm, and the pulse width is also compressed to hundreds of femtosecond by dechirping the laser pulse. This compact-sized, cost-effective laser source can be used in laser micromachining, or as the seeder source for generating much higher power and energy laser for scientific research. For some applications which need femtosecond laser, this laser source can also be compressed to femtosecond regime.

Protein conformational changes are commonly associated with the formation of protein complexes. The non-catalytic death effector domains (DEDs) mediate protein-protein interactions in a variety of cellular processes, including apoptosis, proliferation and migration, and glucose metabolism. Here, using NMR residual dipolar coupling (RDC) data, we report a conformational change in the DED of the phosphoprotein enriched in astrocytes, 15 kDa (PEA-15) protein in the complex with a mitogen-activated protein (MAP) kinase, extracellular regulated kinase 2 (ERK2), which is essential in regulating ERK2 cellular distribution and function in cell proliferation and migration. The most significant conformational change in PEA-15 happens at helices α2, α3, and α4, which also possess the highest flexibility among the six-helix bundle of the DED. This crucial conformational change is modulated by the D/E-RxDL charge-triad motif, one of the prominent structural features of DEDs, together with a number of other electrostatic and hydrogen bonding interactions on the protein surface. Charge-triad motif promotes the optimal orientation of key residues and expands the binding interface to accommodate protein-protein interactions. However, the charge-triad residues are not directly involved in the binding interface between PEA-15 and ERK2.

Nonequilibrium flows are commonly encountered in aerospace engineering applications such as spacecraft reentry, rocket launch, and satellite attitude control. Numerical simulations help greatly in the understanding of nonequilibrium flow, multiscale effects, and the dynamics in these applications. Coupling particle transport and collision within the gas evolution process, the unified gas-kinetic wave-particle (UGKWP) method has been developed for multiscale flow simulation, offering a strategy that strikes a balance between accuracy and efficiency. In the present study, the UGKWP method simulates challenging flow problems with large Knudsen number variations, including supersonic flow around a sphere, hypersonic flow around a space vehicle, nozzle plume into vacuum, and side-jet impingement on the hypersonic flow. The UGKWP accurately captures complex flow structures and has been verified through experimental data or direct simulation Monte Carlo results. Regarding computational cost, the UGKWP method requires only 60 GiB of memory to simulate the three-dimensional space vehicle with 560,593 cells under various flow conditions, which is manageable even on personal workstations. Given its excellent efficiency and accuracy, the UGKWP method shows its substantial benefits in simulating multiscale flow for aerospace engineering applications. [ABSTRACT FROM AUTHOR]