9 results on '"Yueshan Lei"'
Search Results
2. p53 regulates autophagic activity in senescent rat mesenchymal stromal cells
- Author
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Yong Zheng, Chenghua Hu, Chengjun Hu, and Yueshan Lei
- Subjects
0301 basic medicine ,Senescence ,Aging ,Retinoblastoma Protein ,Biochemistry ,Rats, Sprague-Dawley ,ATG12 ,03 medical and health sciences ,Endocrinology ,stomatognathic system ,Autophagy ,Genetics ,Animals ,Phosphorylation ,RNA, Small Interfering ,Molecular Biology ,Cells, Cultured ,Cellular Senescence ,PI3K/AKT/mTOR pathway ,Gene knockdown ,biology ,TOR Serine-Threonine Kinases ,Mesenchymal stem cell ,Retinoblastoma protein ,Mesenchymal Stem Cells ,Cell Biology ,Cell biology ,030104 developmental biology ,Gene Expression Regulation ,Gene Knockdown Techniques ,biology.protein ,Cancer research ,Tumor Suppressor Protein p53 ,Cell aging - Abstract
The tumor suppressor protein p53 is an important player in the regulation of cell senescence, its functions are largely carried out by modulating its downstream genes. Emerging evidence has suggested that senescence and autophagy appear to be regulated by overlapping signaling pathways. Furthermore, autophagy markers have been observed in senescent cells. In this study, we sought to explore the effects of the expression pattern and function of p53 on the activity of autophagy and replicative senescence in bone marrow derived mesenchymal stromal cells (BMSCs). We found that more than 85% of BMSCs stained positive for SA-β-gal at passage 6 (senescent BMSCs) with increased expressions of senescence related genes (p16(ink4a) and p21(waf1)). These results were accompanied by the up-regulation of p53, down-regulation of mammalian target of rapamycin (mTOR) and phosphorylation of Rb. Senescent BMSCs displayed an increased monodansylcadaverine (MDC) staining and autophagy related genes (LC3 and atg12) level compared with BMSCs at passage 2. Knockdown of p53 alleviated the senescent state and reduced autophagic activity during the progression of BMSC senescence, which was accompanied by significantly up-regulated levels of mTOR and phosphorylation of Rb. These results demonstrate that autophagy increases when BMSCs enter the replicative senescence state, and p53 contributes a crucial role in the up-regulation of autophagy in this state.
- Published
- 2016
3. A Rare Variation of the Testicular Blood Supply by the Deferential Artery
- Author
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Xin He, Yueshan Lei, Yong Zheng, and Qianqian Yuan
- Subjects
endocrine system ,medicine.medical_specialty ,urogenital system ,business.industry ,Deferential artery ,Variation ,urologic and male genital diseases ,Surgery ,Umbilical artery ,Variation (linguistics) ,Internal medicine ,medicine ,Cardiology ,Blood supply ,Anatomy ,business - Abstract
Any compression of testicular arteries may lead to loss of spermatogenesis and gonadal hormone production, existence of the variational arteries is accountable in cases of vasoligation, orchidopexy and other surgical approach on them. Anomalies of the testicular blood arteries were observed during dissection of the pelvic cavity in a 68-year-old male cadaver. This report describes a very rare case of lack of testicular arteries. For the blood supply to the testis, thick deferential arteries form some vascular winding and loops and course accompanied by deferent duct to the testis. This case report would serve as ray of light for knowledge of the possible variations of the testicular arteries during surgical procedures.
- Published
- 2016
4. The role of Snf5 in the osteogenic differentiation potential during replicative senescence of rat mesenchymal stromal cells
- Author
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Yueshan Lei, Nana Han, Yong Zheng, Chengjun Hu, and Shangrong Wu
- Subjects
0301 basic medicine ,Senescence ,Aging ,Stromal cell ,Rats, Sprague-Dawley ,03 medical and health sciences ,Histone H3 ,stomatognathic system ,Osteogenesis ,Animals ,Transcription factor ,Cellular Senescence ,Gene knockdown ,Chemistry ,Mesenchymal stem cell ,Cell Differentiation ,Mesenchymal Stem Cells ,SMARCB1 Protein ,Antigens, Differentiation ,Cell biology ,Rats ,RUNX2 ,030104 developmental biology ,embryonic structures ,Stem cell ,Developmental Biology - Abstract
The osteogenic capacities of bone marrow-derived stromal cells (BMSCs) diminish during replicative senescence, and these changes affect the success of therapeutic application of BMSCs. In this study, we sought to explore the molecular mechanisms underlying the osteogenic differentiation capacities that occur during replicative senescence. It is well known that Oct4 is a key transcription factor essential for maintaining differentiation capacities of the stem cells. In this study, we found that BMSCs at passage 6 (replicative senescent BMSCs) showed marked decreases in the osteogenic differentiation potential and the level of Oct4. These were accompanied by reduced levels of Snf5 and histone H3 lysine-4 trimethylation (H3K4me3) in the Oct4 promoter. In BMSCs at passage 2, knockdown of Snf5 diminished expression of Oct4 and disrupted the up-regulation of alkaline phosphatase (ALP) and runt-related transcription factor 2 (Runx2) after osteogenic differentiation induction, which was accompanied by a reduction in Snf5 and H3K4me3 binding to the Oct4 promoter. These findings indicate that the decreased level of Snf5 binding to the promoter region of the Oct4 gene down-regulated the expression of Oct4, which may be the mechanism underlying the decline in osteogenic capacities in replicative senescent BMSCs.
- Published
- 2017
5. Humanistic Value Development Through Extracurricular Activities in Anatomical Education.
- Author
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Liu He, Congli Zhu, Yueshan Lei, Chengjun Hu, Qin Pan, Yan Tang, Yihao Tian, and Yong Zheng
- Subjects
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HUMANISTIC education , *HUMAN anatomy , *EDUCATIONAL benefits , *BLENDED learning , *STUDENT interests - Abstract
Human Anatomy is the first medical specialization course for students of clinical medicine and cultivates the virtues of medical students, helping them to establish the humanitarian values. Therefore, integrating humanistic values education into the course of human anatomy is crucial. In anatomy courses for clinical students, online previews, group-based clinical problembased learning, and theoretical lectures are used in anatomical education which is integrated with extracurricular activities for the education of human values, such as classical medical stories, group-based humanistic value cultivation, a memorial ceremony for "silent mentors", the morphological drawing and basic experimental skills competitions, and public welfare activities. After a year of implementation and investigation analysis, it was shown that students had a higher willingness to participate in the curriculum activities in this blended learning system, and these activities could stimulate students' interest in learning, which is beneficial to the cultivation of professional and humanistic values. [ABSTRACT FROM AUTHOR]
- Published
- 2024
- Full Text
- View/download PDF
6. HPV16 infection regulates RASSF1A transcription mediated by p53
- Author
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Yihao Tian, Yueshan Lei, Chengjun Hu, and Hongxin Xu
- Subjects
Male ,endocrine system ,Cancer Research ,Transcription, Genetic ,Uterine Cervical Neoplasms ,Apoptosis ,Biology ,medicine.disease_cause ,Biochemistry ,Testicular Neoplasms ,Downregulation and upregulation ,RNA interference ,Transcription (biology) ,Cell Line, Tumor ,Genetics ,medicine ,Humans ,E2F1 ,Promoter Regions, Genetic ,Molecular Biology ,Human papillomavirus 16 ,Gene knockdown ,Tumor Suppressor Proteins ,Papillomavirus Infections ,Oncogene Proteins, Viral ,Cell cycle ,female genital diseases and pregnancy complications ,Repressor Proteins ,Protein Transport ,Gene Expression Regulation ,Oncology ,Cancer research ,Molecular Medicine ,Female ,Tumor Suppressor Protein p53 ,Carcinogenesis ,Protein Binding - Abstract
Human papillomavirus (HPV) 16 infection and RASSF1A expression play important roles in tumor development and progression. However, the precise mechanisms underlying their concerted function in the development of reproductive system tumors still remain to be elucidated. In the present study, we showed that HPV16-E6 selectively upregulates RASSF1A expression via degradation of p53, which interacts with the RASSF1A promoter and regulates apoptosis. Overexpression of p53 triggered a decrease in endogenous RASSF1A in SiHa cells, accompanied by apoptosis. Similarly, knockdown of endogenous HPV16-E6 in SiHa cells with RNA interference (RNAi) led to downregulation of RASSF1A mediated by p53 and the subsequent induction of apoptosis. These findings collectively suggest that HPV16 infection regulates p53-mediated RASSF1A expression and suppresses apoptosis. Moreover, RASSF1A may form an element of the negative autoregulatory feedback loops that act on the HPV16 response and are involved in p53-dependent apoptosis. Our results provide novel insights into the cellular mechanism of tumor development, and present a starting point for the development of novel strategies in cancer treatment and effective diagnosis.
- Published
- 2013
7. Inhibition of autophagy alleviates the senescent state of rat mesenchymal stem cells during long-term culture
- Author
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Liu He, Chengjun Hu, Nana Han, Ru-Hong Zhuo, Yong Zheng, and Yueshan Lei
- Subjects
Senescence ,Cancer Research ,Cell ,Vacuole ,Ubiquitin-Activating Enzymes ,Biology ,Biochemistry ,Autophagy-Related Protein 7 ,ATG12 ,Rats, Sprague-Dawley ,Genetics ,medicine ,Autophagy ,Animals ,Molecular Biology ,Cells, Cultured ,Cellular Senescence ,Cyclin-Dependent Kinase Inhibitor p16 ,Mesenchymal stem cell ,Mesenchymal Stem Cells ,Cell cycle ,beta-Galactosidase ,Cell biology ,Rats ,medicine.anatomical_structure ,Oncology ,p21-Activated Kinases ,Small Ubiquitin-Related Modifier Proteins ,Molecular Medicine ,Microtubule-Associated Proteins - Abstract
Following a limited number of cell divisions, mesenchymal stem cells (MSCs) undergo senescence, and these senescent cells maintain metabolic modification and remain viable for long periods. Autophagy, an intracellular bulk degradation process, provides a survival effect for cells under stress. In this study, the effect of autophagy on senescent MSCs was analyzed. Following serial passaging, rat MSCs underwent replicative senescence, characterized by positive staining for senescence-associated β-galactosidase (SA-β-gal), and increased expression levels of p16 and p21. During MSC senescence, the levels of autophagic activity were increased, a greater number of autophagic vacuoles were observed in senescent MSCs by transmission electron microscopy, acridine orange staining was elevated and the expression levels of autophagy‑related proteins (microtubule‑associated protein 1A/1B‑light chain 3-II, Atg7 and Atg12) were increased. The role of autophagy in MSC senescence was further investigated through pharmacological inhibition of autophagy with bafilomycin A1 and 3-methyladenine. Inhibition of autophagy by pharmacological means reduced the rate of positive staining for SA-β-gal and the expression levels of senescence‑related proteins. In conclusion, these findings suggest that autophagy is activated during senescence and the autophagic activity may be a requirement for maintaining the senescent state of MSCs.
- Published
- 2013
8. Responses of vascular smooth muscle cells to estrogen are dependent on balance between ERK and p38 MAPK pathway activities
- Author
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Congli Zhu, Qiao Wang, Yun Zou, Yueshan Lei, Bei Cheng, Chengjun Hu, and Jian Song
- Subjects
MAPK/ERK pathway ,medicine.medical_specialty ,Vascular smooth muscle ,MAP Kinase Signaling System ,p38 mitogen-activated protein kinases ,Myocytes, Smooth Muscle ,Estrogen receptor ,p38 Mitogen-Activated Protein Kinases ,Muscle, Smooth, Vascular ,Rats, Sprague-Dawley ,Internal medicine ,medicine ,Animals ,Extracellular Signal-Regulated MAP Kinases ,Cells, Cultured ,biology ,business.industry ,Kinase ,MEK inhibitor ,Estrogens ,Cell biology ,Rats ,Enzyme Activation ,Endocrinology ,Mitogen-activated protein kinase ,biology.protein ,Phosphorylation ,Female ,Cardiology and Cardiovascular Medicine ,business - Abstract
Objective To investigate the mechanisms underlying the dual effects of estrogen on vascular smooth muscle cells (VSMC). Methods MTT assay, ELISA, flow cytometry and Western analysis were used to investigate the effects of 17β-estradiol (E 2 ) on proliferation, apoptosis, cell cycle progression, ERK and p38 activities of subcultured rat VSMC with or without chemical block of MEK or p38 kinases. Results E 2 -promoted VSMC proliferation was accompanied with an increased phosphorylation of ERK1/2, which could be blocked by MEK inhibitor U0126; the E 2 -induced VSMC apoptosis, which appeared mainly in the G2/M phase, was related with the activation of p38 and could be blocked by p38 inhibitor SB203580. More interestingly, MEK inhibition in E 2 -treated VSMC led to an enhanced p38 phosphorylation and a shift of apoptosis from G2/M phase-predominant to G0/G1 phase-predominant; whereas block of p38 increased the E 2 -induced ERK1/2 phosphorylation and proliferation of the VSMC. This reciprocal phenomenon was related with cross-talk between ERK and p38 pathways which might be mediated by MKP-1 and PP2A. The effects of E 2 on proliferation and apoptosis, and their related pathways could be separately induced by the specific agonists of estrogen receptor (ER) α and β alone and inhibited or eliminated by the ER blocker ICI 182,780. Conclusion The dual effects of estrogen on VSMC involve concurrent activations of ERK and p38 pathways by ER α and β respectively, and the fates of VSMC are determined by the dynamic balance between these two pathways.
- Published
- 2007
9. A Rare Variation of the Testicular Blood Supply by the Deferential Artery.
- Author
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Yong Zheng, Qianqian Yuan, Xin He, and Yueshan Lei
- Subjects
- *
ARTERIAL surgery , *DONOR blood supply , *DISSECTION , *SPERMATOGENESIS , *COMPRESSION therapy ,ARTERIAL abnormalities - Abstract
Any compression of testicular arteries may lead to loss of spermatogenesis and gonadal hormone production, existence of the variational arteries is accountable in cases of vasoligation, orchidopexy and other surgical approach on them. Anomalies of the testicular blood arteries were observed during dissection of the pelvic cavity in a 68-year-old male cadaver. This report describes a very rare case of lack of testicular arteries. For the blood supply to the testis, thick deferential arteries form some vascular winding and loops and course accompanied by deferent duct to the testis. This case report would serve as ray of light for knowledge of the possible variations of the testicular arteries during surgical procedures. [ABSTRACT FROM AUTHOR]
- Published
- 2016
- Full Text
- View/download PDF
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