23 results on '"Yueh-Feng Wu"'
Search Results
2. Integrative Analysis of Myofibroblast Transformation During Corneal Fibrosis.
- Author
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Yun-Ling Pai, Yueh-Feng Wu, and Sung-Jan Lin
- Published
- 2022
- Full Text
- View/download PDF
3. Adaptive 3D cell segmentation and tracing algorithm using convex separation and histogram information for vivo images.
- Author
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Ya-Hsin Cheng, Tzu-Chieh Lin, Jian-Jiun Ding, Yueh-Feng Wu, and Sung-Jan Lin
- Published
- 2017
- Full Text
- View/download PDF
4. Targeting ER protein TXNDC5 in hepatic stellate cell mitigates liver fibrosis by repressing non-canonical TGFβ signalling
- Author
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Chen-Ting Hung, Tung-Hung Su, Yen-Ting Chen, Yueh-Feng Wu, You-Tzung Chen, Sung-Jan Lin, Shuei-Liong Lin, and Kai-Chien Yang
- Subjects
Liver Cirrhosis ,Mice ,Thioredoxins ,Liver ,Gastroenterology ,Hepatic Stellate Cells ,Hepatocytes ,Protein Disulfide-Isomerases ,Animals ,Humans ,Carbon Tetrachloride ,Fibrosis - Abstract
Background and objectivesLiver fibrosis (LF) occurs following chronic liver injuries. Currently, there is no effective therapy for LF. Recently, we identified thioredoxin domain containing 5 (TXNDC5), an ER protein disulfide isomerase (PDI), as a critical mediator of cardiac and lung fibrosis. We aimed to determine if TXNDC5 also contributes to LF and its potential as a therapeutic target for LF.DesignHistological and transcriptome analyses on human cirrhotic livers were performed. Col1a1-GFPTg, Alb-Cre;Rosa26-tdTomato and Tie2-Cre/ERT2;Rosa26-tdTomato mice were used to determine the cell type(s) where TXNDC5 was induced following liver injury. In vitro investigations were conducted in human hepatic stellate cells (HSCs). Col1a2-Cre/ERT2;Txndc5fl/fl (Txndc5cKO) and Alb-Cre;Txndc5fl/fl (Txndc5Hep-cKO) mice were generated to delete TXNDC5 in HSCs and hepatocytes, respectively. Carbon tetrachloride treatment and bile duct ligation surgery were employed to induce liver injury/fibrosis in mice. The extent of LF was quantified using histological, imaging and biochemical analyses.ResultsTXNDC5 was upregulated markedly in human and mouse fibrotic livers, particularly in activated HSC at the fibrotic foci. TXNDC5 was induced by transforming growth factor β1 (TGFβ1) in HSCs and it was both required and sufficient for the activation, proliferation, survival and extracellular matrix production of HSC. Mechanistically, TGFβ1 induces TXNDC5 expression through increased ER stress and ATF6-mediated transcriptional regulation. In addition, TXNDC5 promotes LF by redox-dependent JNK and signal transducer and activator of transcription 3 activation in HSCs through its PDI activity, activating HSCs and making them resistant to apoptosis. HSC-specific deletion of Txndc5 reverted established LF in mice.ConclusionsER protein TXNDC5 promotes LF through redox-dependent HSC activation, proliferation and excessive extracellular matrix production. Targeting TXNDC5, therefore, could be a potential novel therapeutic strategy to ameliorate LF.
- Published
- 2021
5. Intravital multiphoton microscopic imaging platform for ocular surface imaging
- Author
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Yueh-Feng Wu, Chia-Yi Wang, Tsung-Lin Yang, Hsin-Yuan Tan, Sung-Jan Lin, and Po-Nien Tsao
- Subjects
0301 basic medicine ,Genetically modified mouse ,Cell type ,Conjunctiva ,Corneal Stroma ,Diagnostic Techniques, Ophthalmological ,Limbus Corneae ,Green fluorescent protein ,Cornea ,Mice ,03 medical and health sciences ,Cellular and Molecular Neuroscience ,Imaging, Three-Dimensional ,0302 clinical medicine ,In vivo ,medicine ,Animals ,Corneal epithelium ,Chemistry ,Epithelium, Corneal ,eye diseases ,Sensory Systems ,Epithelium ,Cell biology ,Ophthalmology ,Microscopy, Fluorescence, Multiphoton ,030104 developmental biology ,medicine.anatomical_structure ,030221 ophthalmology & optometry ,sense organs - Abstract
The purpose of this study is to provide an intravital noninvasive multiphoton microscopic platform for long-term ocular imaging in transgenic fluorescent mice with subcellular resolution. A multiphoton microscopic system with tunable laser output was employed. We designed a mouse holder incorporated with stereotaxic motorized stage for in vivo three-dimensional imaging of ocular surface in 3 transgenic mouse line with fluorescent protein (FP) expression to visualize distinct structures. With our imaging platform and the expression of FPs, we obtained the three-dimensional images across the whole cornea from epithelium to endothelium and in conjunctiva with subcellular resolution in vivo. Specified EGFP expression in corneal epithelium of K5-H2B-EGFP mice helped to identify both corneal and limbal epithelial cells while ubiquitous nuclear FP expression in R26R-GR mice allowed us to visualized nuclei of all cell types. Universal membrane-localized FP in mT/mG mice outlined all cell boundaries, nerve fibers, and capillaries. The simultaneously collected second harmonic generation signals from collagenous stroma provided architectural contrast. Time-lapsed recording enabled monitoring the mitotic activity of corneal epithelial cells and limbal epithelial cells. We developed an intravital multiphoton microscopic stereotaxic imaging platform and showed that, by incorporating FP-expressing transgenic mice, this platform enables in vivo 4-dimensional ophthalmic study at subcellular resolution.
- Published
- 2019
6. Targeting ER protein TXNDC5 in hepatic stellate cell mitigates liver fibrosis by repressing non-canonical TGFβ signalling.
- Author
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Chen-Ting Hung, Tung-Hung Su, Yen-Ting Chen, Yueh-Feng Wu, You-Tzung Chen, Sung-Jan Lin, Shuei-Liong Lin, and Kai-Chien Yang
- Subjects
KUPFFER cells ,LIVER cells ,HEPATIC fibrosis ,PANCREATIC cysts ,FIBROSIS ,CELL receptors ,EXTRACELLULAR matrix proteins - Published
- 2022
- Full Text
- View/download PDF
7. Endoplasmic reticulum protein TXNDC5 promotes renal fibrosis by enforcing TGF-β signaling in kidney fibroblasts
- Author
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Shuei-Liong Lin, Kai-Chien Yang, Yen-Ting Chen, Sung-Jan Lin, Wen-Chih Chiang, Pei Yu Jhao, Yueh Feng Wu, and Chen Ting Hung
- Subjects
0301 basic medicine ,Nephrology ,medicine.medical_specialty ,Cardiac fibrosis ,Kidney ,Cell Line ,Transforming Growth Factor beta1 ,03 medical and health sciences ,Mice ,0302 clinical medicine ,Thioredoxins ,Downregulation and upregulation ,Fibrosis ,Internal medicine ,medicine ,Renal fibrosis ,Animals ,Mice, Knockout ,medicine.diagnostic_test ,business.industry ,General Medicine ,Fibroblasts ,medicine.disease ,Endoplasmic Reticulum Stress ,Activating Transcription Factor 6 ,Up-Regulation ,030104 developmental biology ,medicine.anatomical_structure ,030220 oncology & carcinogenesis ,Cancer research ,Commentary ,Kidney Diseases ,Renal biopsy ,business ,Kidney disease ,Signal Transduction - Abstract
Renal fibrosis, a common pathological manifestation of virtually all types of chronic kidney disease (CKD), often results in diffuse kidney scarring and predisposes to end-stage renal disease. Currently, there is no effective therapy against renal fibrosis. Recently, our laboratory identified an ER-resident protein, thioredoxin domain containing 5 (TXNDC5), as a critical mediator of cardiac fibrosis. Transcriptome analyses of renal biopsy specimens from patients with CKD revealed marked TXNDC5 upregulation in fibrotic kidneys, suggesting a potential role of TXNDC5 in renal fibrosis. Employing multiple fluorescence reporter mouse lines, we showed that TXNDC5 was specifically upregulated in collagen-secreting fibroblasts in fibrotic mouse kidneys. In addition, we showed that TXNDC5 was required for TGF-β1-induced fibrogenic responses in human kidney fibroblasts (HKFs), whereas TXNDC5 overexpression was sufficient to promote HKF activation, proliferation, and collagen production. Mechanistically, we showed that TXNDC5, transcriptionally controlled by the ATF6-dependent ER stress pathway, mediated its profibrogenic effects by enforcing TGF-β signaling activity through posttranslational stabilization and upregulation of type I TGF-β receptor in kidney fibroblasts. Using a tamoxifen-inducible, fibroblast-specific Txndc5 knockout mouse line, we demonstrated that deletion of Txndc5 in kidney fibroblasts mitigated the progression of established kidney fibrosis, suggesting the therapeutic potential of TXNDC5 targeting for renal fibrosis and CKD.
- Published
- 2020
8. Fibroblast-enriched endoplasmic reticulum protein TXNDC5 promotes pulmonary fibrosis by augmenting TGFβ signaling through TGFBR1 stabilization
- Author
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Shuei-Liong Lin, Shen-Chuan Lo, Yu-Shan Lin, Y.-H. Lee, Frank-Leigh Lu, Kai-Chien Yang, Sung-Jan Lin, Tzu-Pin Shentu, Pei-Chen Wu, Chen-Ting Hung, Ying-Chun Shih, Robert D. Guzy, Chau-Chung Wu, Wan-Lin Wu, Yen-Ting Chen, Tzu-Han Lee, Anne I. Sperling, Ru-Ting Huang, Ming-Yi You, Yueh-Feng Wu, Chiung-Nien Chen, Yun Fang, Po-Nien Tsao, Chih-Fan Yeh, Yi-Shuan Tseng, and Tzu-Hung Lin
- Subjects
0301 basic medicine ,Male ,Protein Folding ,Molecular biology ,Pulmonary Fibrosis ,Receptor, Transforming Growth Factor-beta Type I ,General Physics and Astronomy ,02 engineering and technology ,Idiopathic pulmonary fibrosis ,chemistry.chemical_compound ,Mice ,Thioredoxins ,Pulmonary fibrosis ,Protein disulfide-isomerase ,lcsh:Science ,Mice, Knockout ,Multidisciplinary ,Protein Stability ,021001 nanoscience & nanotechnology ,Endoplasmic Reticulum Stress ,Up-Regulation ,medicine.anatomical_structure ,0210 nano-technology ,Signal Transduction ,Science ,Protein Disulfide-Isomerases ,Bleomycin ,General Biochemistry, Genetics and Molecular Biology ,Article ,Transforming Growth Factor beta1 ,03 medical and health sciences ,Downregulation and upregulation ,medicine ,Animals ,Humans ,Respiratory tract diseases ,Lung ,business.industry ,Endoplasmic reticulum ,General Chemistry ,medicine.disease ,Idiopathic Pulmonary Fibrosis ,Mice, Inbred C57BL ,Disease Models, Animal ,030104 developmental biology ,chemistry ,Unfolded protein response ,Cancer research ,lcsh:Q ,business ,Gene Deletion - Abstract
Pulmonary fibrosis (PF) is a major public health problem with limited therapeutic options. There is a clear need to identify novel mediators of PF to develop effective therapeutics. Here we show that an ER protein disulfide isomerase, thioredoxin domain containing 5 (TXNDC5), is highly upregulated in the lung tissues from both patients with idiopathic pulmonary fibrosis and a mouse model of bleomycin (BLM)-induced PF. Global deletion of Txndc5 markedly reduces the extent of PF and preserves lung function in mice following BLM treatment. Mechanistic investigations demonstrate that TXNDC5 promotes fibrogenesis by enhancing TGFβ1 signaling through direct binding with and stabilization of TGFBR1 in lung fibroblasts. Moreover, TGFβ1 stimulation is shown to upregulate TXNDC5 via ER stress/ATF6-dependent transcriptional control in lung fibroblasts. Inducing fibroblast-specific deletion of Txndc5 mitigates the progression of BLM-induced PF and lung function deterioration. Targeting TXNDC5, therefore, could be a novel therapeutic approach against PF., Pulmonary fibrosis is a major public health problem with unclear mechanism and limited therapeutic options. Here the authors show that a fibroblast-enriched endoplasmic reticulum protein, TXNDC5, promotes pulmonary fibrosis by stabilizing TGFBR1 and show the potential of TXNDC5 as a therapeutic target against pulmonary fibrosis.
- Published
- 2020
9. A Custom Multiphoton Microscopy Platform for Live Imaging of Mouse Cornea and Conjunctiva
- Author
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Yueh-Feng Wu, Rai-Teng Ye, Ming-Kai Pan, Sung-Jan Lin, and Hsin-Yuan Tan
- Subjects
Conjunctiva ,Materials science ,Stromal cell ,General Immunology and Microbiology ,General Chemical Engineering ,General Neuroscience ,Intravital Imaging ,eye diseases ,General Biochemistry, Genetics and Molecular Biology ,Cornea ,Mice ,medicine.anatomical_structure ,Multiphoton fluorescence microscope ,Microscopy, Fluorescence, Multiphoton ,In vivo ,Live cell imaging ,Microscopy ,medicine ,Animals ,sense organs ,Biomedical engineering - Abstract
Conventional histological analysis and cell culture systems are insufficient to simulate in vivo physiological and pathological dynamics completely. Multiphoton microscopy (MPM) has become one of the most popular imaging modalities for biomedical study at cellular levels in vivo, advantages include high resolution, deep tissue penetration and minimal phototoxicity. We have designed an MPM imaging platform with a customized mouse eye holder and a stereotaxic stage for imaging ocular surface in vivo. Dual fluorescent protein reporter mouse enables visualization of cell nuclei, cell membranes, nerve fibers, and capillaries within the ocular surface. In addition to multiphoton fluorescence signals, acquiring second harmonic generation (SHG) simultaneously allows for the characterization of collagenous stromal architecture. This platform can be employed for intravital imaging with accurate positioning across the entire ocular surface, including cornea and conjunctiva.
- Published
- 2020
10. 3-D Cell Segmentation by Improved V-Net Architecture Using Edge and Boundary Labels
- Author
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Jian-Jiun Ding, Yueh-Feng Wu, Sung-Jan Lin, Ping-Hung Chen, and Chieh-Sheng Chang
- Subjects
Computer science ,business.industry ,Deep learning ,media_common.quotation_subject ,020208 electrical & electronic engineering ,Boundary (topology) ,020206 networking & telecommunications ,Pattern recognition ,02 engineering and technology ,Image segmentation ,Net (mathematics) ,Convolutional neural network ,0202 electrical engineering, electronic engineering, information engineering ,Contrast (vision) ,Segmentation ,Enhanced Data Rates for GSM Evolution ,Artificial intelligence ,business ,media_common - Abstract
Cell image segmentation is an important topic since it benefits for medical research and diagnosis. It is more challenging than other segmentation problems because cells have similar colors and their boundaries are not always obvious. In recent years, deep learning based methods, including the V-net, play an important role in image segmentation. In this paper, we proposed several techniques to further improve the performance of the V-net for cell segmentation. First, in addition to (i) cells and (ii) background, we add two labels: (iii) the edge between cells and background and (iv) the edges among cells. Since the properties of cell edges are quite different from those of cell bodies and background, adding these labels are helpful for improving the performance. Moreover, several morphology-based post-processing algorithms are applied for boundary refinement. With these techniques, the accuracies of cell segmentation can be much improved and the cells with poor contrast can still be well segmented.
- Published
- 2019
11. Long-Term Intravital Imaging of the Cornea, Skin, and Hair Follicle by Multiphoton Microscope
- Author
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Yueh-Feng, Wu, Hsin-Yuan, Tan, and Sung-Jan, Lin
- Subjects
Cornea ,Imaging, Three-Dimensional ,Microscopy, Fluorescence, Multiphoton ,Intravital Microscopy ,Animals ,Mice, Transgenic ,Epidermis ,Hair Follicle ,Skin - Abstract
Multiphoton microscopy allows long-term direct visualization of cells in live animals due to its low photodamage. When coupled with fluorescence protein targeting and second harmonic generation signals from natural collagen as contrast, multiphoton microscopy enables intravital tracing of cells while providing structural information from the extracellular matrix. Compared with conventional histological analysis, it can bring new insight into the cell dynamics in stem cell research. Here, we demonstrate cell imaging and tracing at a single cell resolution in the cornea, skin, and hair follicles using multiphoton microscopy in transgenic mice of which specific cell populations are tagged with fluorescent proteins.
- Published
- 2019
12. Long-Term Intravital Imaging of the Cornea, Skin, and Hair Follicle by Multiphoton Microscope
- Author
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Hsin-Yuan Tan, Yueh-Feng Wu, and Sung-Jan Lin
- Subjects
0301 basic medicine ,Microscope ,integumentary system ,Chemistry ,Cell ,Resolution (electron density) ,Hair follicle ,Fluorescence ,law.invention ,Extracellular matrix ,03 medical and health sciences ,030104 developmental biology ,0302 clinical medicine ,medicine.anatomical_structure ,law ,Cornea ,medicine ,Biophysics ,sense organs ,Stem cell ,030217 neurology & neurosurgery - Abstract
Multiphoton microscopy allows long-term direct visualization of cells in live animals due to its low photodamage. When coupled with fluorescence protein targeting and second harmonic generation signals from natural collagen as contrast, multiphoton microscopy enables intravital tracing of cells while providing structural information from the extracellular matrix. Compared with conventional histological analysis, it can bring new insight into the cell dynamics in stem cell research. Here, we demonstrate cell imaging and tracing at a single cell resolution in the cornea, skin, and hair follicles using multiphoton microscopy in transgenic mice of which specific cell populations are tagged with fluorescent proteins.
- Published
- 2019
13. Chidamide-induced ROS accumulation and miR-129-3p-dependent cell cycle arrest in non-small lung cancer cells
- Author
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Bing-Yen Wang, Hui-Yi Chang, Chu-Chyn Ou, Jiunn-Liang Ko, Yueh-Feng Wu, and Peng-Ju Chien
- Subjects
Telomerase ,Cell cycle checkpoint ,Lung Neoplasms ,medicine.drug_class ,Pharmaceutical Science ,Aminopyridines ,Antineoplastic Agents ,Flow cytometry ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Chidamide ,Carcinoma, Non-Small-Cell Lung ,Cell Line, Tumor ,Drug Discovery ,medicine ,Humans ,Telomerase reverse transcriptase ,030304 developmental biology ,Pharmacology ,A549 cell ,0303 health sciences ,medicine.diagnostic_test ,Chemistry ,Histone deacetylase inhibitor ,Transfection ,Cell Cycle Checkpoints ,Gene Expression Regulation, Neoplastic ,Histone Deacetylase Inhibitors ,MicroRNAs ,Complementary and alternative medicine ,A549 Cells ,030220 oncology & carcinogenesis ,Benzamides ,Cancer research ,Molecular Medicine ,Reactive Oxygen Species - Abstract
Background Epigenetic therapy is a promising popular treatment modality for various cancers. Histone modification and miRNA should not be underestimated in lung cancer. This study aimed to investigate whether chidamide, a histone deacetylase inhibitor (HDACi), which inhibits telomerase activity and induces cell cycle arrest, influences ROS and miRNA production in non-small cell lung cancer (NSCLC) cells. Methods H1355 and A549 were treated with chidamide. The analysis of DNA content was measured by FACSCalibur equipped with a 488 nm laser. H1355 cells were transfected with miR-129-3p mimic by Lipofectamine2000. Telomerase activity was performed on the telomeric repeat amplification protocol (TRAP) assay. Detection of thymidylate synthase (TS), p21, p53, pRB, and β-actin, were performed by western blot analysis. Results Our data showed that expression of TS, p21, and pRB were altered in the presence of chidamide by PCR and western blot. Using BrdU-incorporation analysis, we found that chidamide induced G1 arrest through the regulation of the TS gene by miR-129-3p. Chidamide was shown to suppress telomerase activity in the TRAP assay and reduced the expression of human telomerase reverse transcriptase (hTERT) by PCR and q-PCR in H1355 and A549 cells. Chidamide increased the generation of reactive oxygen species (ROS) by flow cytometry. N-acetyl cysteine (NAC), a ROS scavenger, attenuated chidamide-induced telomerase activity inhibition. Conclusion Chidamide repressed telomerase activity through ROS accumulation and cell cycle arrest by miR-129-3p upregulation in both H1355 and A549 cells. This is the first study to demonstrate that chidamide induces miR-129-3p upregulation and ROS accumulation, leading to cell cycle arrest.
- Published
- 2018
14. Cell Segmentation Algorithm Using Double Thresholding with Morphology-Based Techniques
- Author
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Yueh-Feng Wu, Jian-Jiun Ding, Sung-Jan Lin, and Chieh-Sheng Chang
- Subjects
Computer science ,business.industry ,Cell segmentation ,Morphology (biology) ,Pattern recognition ,Artificial intelligence ,business ,Thresholding - Abstract
Cell segmentation has attracted increasing interests since it is helpful for medical research and automatic diagnosis. In this paper, we proposed a method that can well segment cells by applying two stages of thresholds, a global one and an adaptive one, with morphology operations to overcome overlapping problems of cells.
- Published
- 2018
15. Enhancing hair follicle regeneration by nonablative fractional laser: Assessment of irradiation parameters and tissue response
- Author
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Tsung-Hua Tsai, Hsien-Yi Chiu, Shiou-Han Wang, Sung-Jan Lin, Sabrina Mai-Yi Fan, Yueh-Feng Wu, and Pei-Shan Wu
- Subjects
medicine.medical_specialty ,Pathology ,integumentary system ,Chemistry ,Regeneration (biology) ,Fractional laser ,Inflammation ,Dermatology ,Laser ,Hair follicle ,law.invention ,Proinflammatory cytokine ,medicine.anatomical_structure ,law ,medicine ,Surgery ,Irradiation ,medicine.symptom ,Stem cell - Abstract
Background and Objective Identification of methods to enhance anagen entry can be helpful for alopecia. Recently, nonablative laser has been proposed as a potential treatment for alopecia. However, how the laser parameters affect stem cell activity, hair cycles and the associated side effects have not been well characterized. Here we examine the effects of irradiation parameters of 1,550-nm fractional laser on hair cycles. Study Design/Materials and Methods The dorsal skin of eight-week-old female C57BL/6 mice with hair follicles in synchronized telogen was shaved and irradiated with a 1,550-nm fractional erbium-glass laser (Fraxel RE:STORE (SR1500) Laser System, Solta Medical, U.S.A.) with varied beam energies (5–35 mJ) and beam densities (500–3500 microthermal zones/cm2). The cutaneous changes were evaluated both grossly and histologically. Hair follicle stem cell activity was detected by BrdU incorporation and changes in gene expression were quantified by real-time PCR. Results Direct thermal injury to hair follicles could be observed early after irradiation, especially at higher beam energy. Anagen induction in the irradiated skin showed an all-or-non change. Anagen induction and ulcer formation were affected by the combination of beam energy and density. The lowest beam energy of 5 mJ failed to promote anagen entry at all beam densities tested. As beam energy increased from 10 mJ to 35 mJ, we found a decreasing trend of beam density that could induce anagen entry within 7–9 days with activation of hair follicle stem cells. Beam density above the pro-regeneration density could lead to ulcers and scarring followed by anagen entry in adjacent skin. Analysis of inflammatory cytokines, including TNF-α, IL-1β, and IL-6, revealed that transient moderate inflammation was associated with anagen induction and intense prolonged inflammation preceded ulcer formation. Conclusion To avoid side effects of hair follicle injury and scarring, appropriate combination of beam energy and density is required. Parameters outside the therapeutic window can result in either no anagen promotion or ulcer formation. Lasers Surg. Med. 47:331–341, 2015. © 2015 Wiley Periodicals, Inc.
- Published
- 2015
16. Visible red light enhances physiological anagen entry in vivo and has direct and indirect stimulative effects in vitro
- Author
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Sabrina Mai-Yi Fan, Yi-Shuan Sheen, Chih-Chieh Chan, Shiou-Hwa Jee, Sung-Jan Lin, and Yueh-Feng Wu
- Subjects
integumentary system ,Cell growth ,Dermatology ,Biology ,Outer root sheath ,Cell biology ,Paracrine signalling ,Dermal papillae ,medicine.anatomical_structure ,Hair cycle ,Immunology ,medicine ,Fibroblast Growth Factor 7 ,Extracellular ,Surgery ,Keratinocyte - Abstract
Background and Objectives Hair follicles are located at the interface of the external and internal environments and their cycling has been shown to be regulated by intra- and extra-follicular factors. The aim of this study is to examine whether or how hair follicles respond to visible light. Study Design/Materials and Methods We examined the effect of 3 mW red (630 nm, 1 J/cm2), 2 mW green (522 nm, 1 J/cm2), and 2 mW blue light (463 nm, 1 J/cm2) on telogen in mice for 3 weeks. The photobiologic effects of red light on cell proliferation of outer root sheath keratinocytes and dermal papilla cells were studied in vitro. Results We found that red light accelerated anagen entry faster than green and blue light in mice. Red light irradiation stimulated the proliferation of both outer root sheath keratinocytes and dermal papilla cells in a dose-dependent manner by promoting cell cycle progression. This stimulative effect was mediated via extracellular signal-regulated kinase phosphorylation in both cells. In a co-culture condition, dermal papilla cells irradiated by red light further enhanced keratinocyte proliferation, suggesting enhanced epithelial-mesenchymal interaction. In search for factors that mediated this paracrine effect, we found fibroblast growth factor 7 was upregulated in both mRNA and protein levels. The stimulative paracrine effect on keratinocytes was significantly inhibited by neutralizing antibody against fibroblast growth factor 7. Conclusions These results suggest that hair follicles respond to visible light in vivo. Red light may promote physiological telogen to anagen transition by directly stimulating outer root sheath keratinocytes and indirectly by enhancing epithelial-mesenchymal interaction in vitro. Lasers Surg. Med. 47:50–59, 2015. © 2014 Wiley Periodicals, Inc.
- Published
- 2014
17. Adaptive 3D cell segmentation and tracing algorithm using convex separation and histogram information for vivo images
- Author
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Yueh-Feng Wu, Ya-Hsin Cheng, Tzu-Chieh Lin, Sung-Jan Lin, and Jian-Jiun Ding
- Subjects
0301 basic medicine ,business.industry ,Segmentation-based object categorization ,Computer science ,Cell segmentation ,Scale-space segmentation ,Pattern recognition ,Image segmentation ,Thresholding ,03 medical and health sciences ,030104 developmental biology ,Histogram ,Path (graph theory) ,Shortest path problem ,Segmentation ,Computer vision ,Artificial intelligence ,business ,Algorithm - Abstract
In biology and medicine, it is critical to study the amount, distribution, movement, status, and behavior of cells. To do this, one should well segment cells from three dimensional (3D) cell images in prior and precisely extract the moving path of each cell. In this work, an advanced 3D cell segmentation and tracing algorithm is proposed. It applies local histograms to perform adaptive thresholding. Moreover, based on the observation that a cell is always nearly convex, the technique of shortest path segmentation is applied to disconnect the over-merged cells. Furthermore, the overlapping cell areas between two adjacent layers are adopted to refine segmentation results. Then, the moving path of a cell can be traced by determining the cell with the shortest distance and the most similar characteristics in the next 3D image. With the proposed algorithm, 3D cell images can be accurately segmented and each cell can be well traced.
- Published
- 2017
18. Chidamide alleviates TGF-β-induced epithelial-mesenchymal transition in lung cancer cell lines
- Author
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Sheng-Hao Lin, Bing-Yen Wang, Jiunn-Liang Ko, Peng-Ju Chien, Yueh-Feng Wu, Ching-Hsiung Lin, and Jeremy J.W. Chen
- Subjects
0301 basic medicine ,Epithelial-Mesenchymal Transition ,Lung Neoplasms ,medicine.drug_class ,Bisulfite sequencing ,Cell ,Aminopyridines ,Gene Expression ,Antineoplastic Agents ,Biology ,Epigenesis, Genetic ,03 medical and health sciences ,chemistry.chemical_compound ,Antigens, CD ,Transforming Growth Factor beta ,Chidamide ,Genetics ,medicine ,Humans ,Epithelial–mesenchymal transition ,Phosphorylation ,Histone H3 acetylation ,Promoter Regions, Genetic ,Molecular Biology ,Histone deacetylase inhibitor ,General Medicine ,DNA Methylation ,Cadherins ,Histone H3 deacetylation ,Molecular biology ,Gene Expression Regulation, Neoplastic ,Histone Deacetylase Inhibitors ,030104 developmental biology ,medicine.anatomical_structure ,chemistry ,A549 Cells ,Cancer cell ,Benzamides ,Cancer research ,Drug Screening Assays, Antitumor ,Protein Processing, Post-Translational - Abstract
Transforming growth factor-β (TGF-β)-induced epithelial–mesenchymal transition is a critical process in the initiation of metastasis of various types of cancer. Chidamide is a class I histone deacetylase inhibitor with anti-tumor activity. This study investigated the effects of chidamide on TGF-β-mediated suppression of E-cadherin expression in adenocarcinomic lung epithelial cells and the molecular mechanisms involved in these effects. Western blot analysis, confocal microscopy, Quantitative methyl-specific PCR and bisulfite sequencing were used to evaluate the effects of different treatments on chidamide ameliorating TGF-β induced-E-cadherin loss. H3 acetylation binding to the promoter of E-cadherin was detected by chromatin immunoprecipitations (CHIP). We found that chidamide reduced the level of lung cancer cell migration observed using a Boyden chamber assay (as an indicator of metastatic potential). Chidamide inhibited TGF-β-induced SMAD2 phosphorylation and attenuated TGF-β-induced loss of E-cadherin expression in lung cancer cells by Western blotting and confocal microscopy, respectively. Quantitative methyl-specific PCR and bisulfite sequencing revealed that TGF-β-enhanced E-cadherin promoter methylation was ameliorated in cells treated with chidamide. We demonstrated that histone H3 deacetylation within the E-cadherin promoter was required for TGF-β-induced E-cadherin loss; cell treatment with chidamide increased the H3 acetylation detected by CHIP. Taken together, our results demonstrate that TGF-β suppressed E-cadherin expression by regulating promoter methylation and histone H3 acetylation. Chidamide significantly enhanced E-cadherin expression in TGF-β-treated cells and inhibited lung cancer cell migration. These findings indicate that chidamide has a potential therapeutic use due to its capacity to prevent cancer cell metastasis.
- Published
- 2015
19. Enhancing hair follicle regeneration by nonablative fractional laser: Assessment of irradiation parameters and tissue response
- Author
-
Yueh-Feng, Wu, Shiou-Han, Wang, Pei-Shan, Wu, Sabrina Mai-Yi, Fan, Hsien-Yi, Chiu, Tsung-Hua, Tsai, and Sung-Jan, Lin
- Subjects
Inflammation ,Mice, Inbred C57BL ,Cicatrix ,Stem Cells ,Skin Ulcer ,Animals ,Cytokines ,Regeneration ,Alopecia ,Female ,Laser Therapy ,Real-Time Polymerase Chain Reaction ,Hair Follicle - Abstract
Identification of methods to enhance anagen entry can be helpful for alopecia. Recently, nonablative laser has been proposed as a potential treatment for alopecia. However, how the laser parameters affect stem cell activity, hair cycles and the associated side effects have not been well characterized. Here we examine the effects of irradiation parameters of 1,550-nm fractional laser on hair cycles.The dorsal skin of eight-week-old female C57BL/6 mice with hair follicles in synchronized telogen was shaved and irradiated with a 1,550-nm fractional erbium-glass laser (Fraxel RE:STORE (SR1500) Laser System, Solta Medical, U.S.A.) with varied beam energies (5-35 mJ) and beam densities (500-3500 microthermal zones/cm(2) ). The cutaneous changes were evaluated both grossly and histologically. Hair follicle stem cell activity was detected by BrdU incorporation and changes in gene expression were quantified by real-time PCR.Direct thermal injury to hair follicles could be observed early after irradiation, especially at higher beam energy. Anagen induction in the irradiated skin showed an all-or-non change. Anagen induction and ulcer formation were affected by the combination of beam energy and density. The lowest beam energy of 5 mJ failed to promote anagen entry at all beam densities tested. As beam energy increased from 10 mJ to 35 mJ, we found a decreasing trend of beam density that could induce anagen entry within 7-9 days with activation of hair follicle stem cells. Beam density above the pro-regeneration density could lead to ulcers and scarring followed by anagen entry in adjacent skin. Analysis of inflammatory cytokines, including TNF-α, IL-1β, and IL-6, revealed that transient moderate inflammation was associated with anagen induction and intense prolonged inflammation preceded ulcer formation.To avoid side effects of hair follicle injury and scarring, appropriate combination of beam energy and density is required. Parameters outside the therapeutic window can result in either no anagen promotion or ulcer formation.
- Published
- 2014
20. Visible red light enhances physiological anagen entry in vivo and has direct and indirect stimulative effects in vitro
- Author
-
Yi-Shuan, Sheen, Sabrina Mai-Yi, Fan, Chih-Chieh, Chan, Yueh-Feng, Wu, Shiou-Hwa, Jee, and Sung-Jan, Lin
- Subjects
Keratinocytes ,Fibroblast Growth Factor 7 ,Light ,Dermis ,In Vitro Techniques ,Mice, Inbred C57BL ,Mice ,Random Allocation ,Animals ,Female ,Hair Follicle ,Biomarkers ,Cell Proliferation ,Hair - Abstract
Hair follicles are located at the interface of the external and internal environments and their cycling has been shown to be regulated by intra- and extra-follicular factors. The aim of this study is to examine whether or how hair follicles respond to visible light.We examined the effect of 3 mW red (630 nm, 1 J/cm(2)), 2 mW green (522 nm, 1 J/cm(2)), and 2 mW blue light (463 nm, 1 J/cm(2)) on telogen in mice for 3 weeks. The photobiologic effects of red light on cell proliferation of outer root sheath keratinocytes and dermal papilla cells were studied in vitro.We found that red light accelerated anagen entry faster than green and blue light in mice. Red light irradiation stimulated the proliferation of both outer root sheath keratinocytes and dermal papilla cells in a dose-dependent manner by promoting cell cycle progression. This stimulative effect was mediated via extracellular signal-regulated kinase phosphorylation in both cells. In a co-culture condition, dermal papilla cells irradiated by red light further enhanced keratinocyte proliferation, suggesting enhanced epithelial-mesenchymal interaction. In search for factors that mediated this paracrine effect, we found fibroblast growth factor 7 was upregulated in both mRNA and protein levels. The stimulative paracrine effect on keratinocytes was significantly inhibited by neutralizing antibody against fibroblast growth factor 7.These results suggest that hair follicles respond to visible light in vivo. Red light may promote physiological telogen to anagen transition by directly stimulating outer root sheath keratinocytes and indirectly by enhancing epithelial-mesenchymal interaction in vitro.
- Published
- 2014
21. Acute urinary retention as a manifestation of emphysematous cystitis
- Author
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Chung-Cheng Kao, Yueh-Feng Wu, Yi-Wei Tsuei, and Jiunn-Bin Hung
- Subjects
Aged, 80 and over ,Emphysema ,Male ,medicine.medical_specialty ,Urinary retention ,business.industry ,Urology ,General Medicine ,Cystoscopy ,Urinary Retention ,medicine.disease ,Anti-Bacterial Agents ,Klebsiella pneumoniae ,Emphysematous cystitis ,Cystitis ,Emergency Medicine ,medicine ,Humans ,medicine.symptom ,business ,Therapeutic Irrigation - Published
- 2014
22. An efficient method for isolation of hair papillae and follicle epithelium from human scalp specimens
- Author
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Hsien-Ching Chiu, Chun‐Hsiang Chang, and Yueh-Feng Wu
- Subjects
Pathology ,medicine.medical_specialty ,Histocytological Preparation Techniques ,Isolation (health care) ,Dermatology ,Anatomy ,Biology ,Epithelium ,Follicle ,medicine.anatomical_structure ,Scalp ,medicine ,Humans ,Hair - Published
- 1993
23. Endoplasmic reticulum protein TXNDC5 promotes renal fibrosis by enforcing TGF-β signaling in kidney fibroblasts.
- Author
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Yen-Ting Chen, Pei-Yu Jhao, Chen-Ting Hung, Yueh-Feng Wu, Sung-Jan Lin, Wen-Chih Chiang, Shuei-Liong Lin, Kai-Chien Yang, Chen, Yen-Ting, Jhao, Pei-Yu, Hung, Chen-Ting, Wu, Yueh-Feng, Lin, Sung-Jan, Chiang, Wen-Chih, Lin, Shuei-Liong, and Yang, Kai-Chien
- Subjects
- *
RENAL fibrosis , *ENDOPLASMIC reticulum , *FIBROBLASTS , *FIBROSIS , *HEART fibrosis , *KIDNEYS - Abstract
Renal fibrosis, a common pathological manifestation of virtually all types of chronic kidney disease (CKD), often results in diffuse kidney scarring and predisposes to end-stage renal disease. Currently, there is no effective therapy against renal fibrosis. Recently, our laboratory identified an ER-resident protein, thioredoxin domain containing 5 (TXNDC5), as a critical mediator of cardiac fibrosis. Transcriptome analyses of renal biopsy specimens from patients with CKD revealed marked TXNDC5 upregulation in fibrotic kidneys, suggesting a potential role of TXNDC5 in renal fibrosis. Employing multiple fluorescence reporter mouse lines, we showed that TXNDC5 was specifically upregulated in collagen-secreting fibroblasts in fibrotic mouse kidneys. In addition, we showed that TXNDC5 was required for TGF-β1-induced fibrogenic responses in human kidney fibroblasts (HKFs), whereas TXNDC5 overexpression was sufficient to promote HKF activation, proliferation, and collagen production. Mechanistically, we showed that TXNDC5, transcriptionally controlled by the ATF6-dependent ER stress pathway, mediated its profibrogenic effects by enforcing TGF-β signaling activity through posttranslational stabilization and upregulation of type I TGF-β receptor in kidney fibroblasts. Using a tamoxifen-inducible, fibroblast-specific Txndc5 knockout mouse line, we demonstrated that deletion of Txndc5 in kidney fibroblasts mitigated the progression of established kidney fibrosis, suggesting the therapeutic potential of TXNDC5 targeting for renal fibrosis and CKD. [ABSTRACT FROM AUTHOR]
- Published
- 2021
- Full Text
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