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ObjectiveTo summarize the clinical characteristics and treatment options together with the maternal and neonatal prognoses in women with different degrees of thrombocytopenia of unknown causes during pregnancy.Materials and MethodsOne hundred twenty-nine cases meeting the inclusion and exclusion criteria were retrospectively analyzed. Patients were divided into group A (50*109/L) and group B (50*109/L to 100*109/L) according to the lowest level of platelet count during pregnancy. Patients were divided into those found to have thrombocytopenia in the relatively early, middle, and late stages according to the detection period of maternal thrombocytopenia during pregnancy.ResultsThere were 72 cases in group A, and 57 cases in group B. There existed statistically significant differences in terms of the proportion of primipara, the proportion with a history of thrombocytopenia, and the median length of pregnancy between the two groups (p p p ConclusionPatients with platelet counts below 50*109/L were mostly primipara with a history of thrombocytopenia, most often detected at a relatively early stage of pregnancy, and continued pregnancy might lead to aggravation of the disease. Combination therapy was required for patients with platelet counts below 30*109/L to maintain the platelet counts within a safe range. Cesarean delivery was selected to terminate the pregnancies, and platelet counts should be raised above 50*109/L before surgery. Close monitoring was required for those with platelet counts above 30*109/L. There was no direct correlation between the maternal and neonatal platelet counts.

Background Unexplained recurrent spontaneous abortion (URSA) frustrates women of childbearing age profoundly, and effective therapies are particularly important. Objective To compare the efficacy of heparin combined with aspirin and aspirin alone for URSA. Search strategy Electronic databases (PubMed, Medline, Web of Science, Clinical key and Cochrane Library) were searched for relevant studies from database inception to August 2019. Selection criteria Studies of women of childbearing age with at least two consecutive abortions were included. Data collection and analysis Relevant items were extracted, tabulated, and subjected to STATA for data analysis. Study women were divided into group A (taking heparin plus aspirin) and group B (taking aspirin alone). The primary outcome was the rate of live birth. Main results Women from eight randomized controlled trials were included: 493 in group A and 501 in group B. The number of live births was significantly higher in group A (P=0.003). The result remained the same in subgroup analysis by presence of antiphospholipid antibodies. Among women who had a live birth, gestational age at delivery tended to be older in group B (P=0.054). No differences in birthweight or intrauterine growth restriction were observed. Adverse effects were sporadically reported. Conclusion Among women with URSA, heparin combined with aspirin increased the live birth rate as compared with aspirin alone. There was a beneficial tendency of taking aspirin-only to prolong gestation week.

The spindle and kinetochore-associated complex subunit 1(SKA1) is a newly discovered gene, which has been associated with mitosis and tumorigenesis. However, its role insalivary adenoid cystic carcinoma (SACC) is still unknown, and the invasive and metastatic mechanism in SACC is still unclear. To explore the molecular mechanism of SKA1 in the process of malignant proliferation and metastasis in adenoid cystic cancer (ACC) cells, we employed lentivirus-mediated short hairpin RNA to knockdown SKA1 in SACC-83 cells. The results demonstrated that the lentivirus-mediated shRNA-targeting SKA1 lead to a significant down-regulation of SKA1 expression. Knockdown of SKA1 inhibited cell proliferation, cell invasion, migration and the cell cycle arrest. Furthermore, knockdown of SKA1 reduced the Ndc80, CDK4, Cyclin D1, Cyclin E1, Cyclin B1 and matrix metalloproteinase-9 (MMP-9) protein expression, but increased the p27 protein expression. These findings indicated that SKA1 might be a promising target for cancer gene therapy in human ACC.

Background: It has been found that L-carnitine ameliorated cisplatin-induced acute kidney injury (AKI) in rats. However, the detailed role of L-carnitine in improving the renal urinary concentration function in cisplatin-induced AKI is not fully understood. Methods: In this study, 30 Sprague-Dawley rats were divided randomly into 5 groups: control, cisplatin (CIS), L-carnitine (CAR), L-carnitine plus cisplatin (CAR + CIS), and cisplatin plus L-carnitine (CIS + CAR) groups. Cisplatin (7 mg/kg) and L-carnitine (300 mg/kg) were injected intraperitoneally. Urine (24 h) and blood samples were collected to analyze renal urinary concentrating function. Immunoblotting, confocal laser microscopy, and enzyme-linked immunosorbent assays were used to assess the level and localization of the water channel aquaporin (AQP) 2, and levels of stimulatory G protein α subunit (GSα protein), arginine vasopressin (AVP) receptor 2, adenylyl cyclase and serum AVP. Results: Renal urinary concentrating function was improved by L-carnitine in rats with cisplatin-induced AKI. AQP2 expression, which decreased after cisplatin treatment, was improved by L-carnitine in different regions of the kidney. Moreover, our data indicated that L-carnitine could increase AQP2 accumulation at the apical plasma membranes of the renal-collecting ducts. Finally, intervention with L-carnitine effectively improved the expression of AQP2 upstream signaling proteins, such as GSα protein, adenylyl cyclase, and serum AVP levels in rats with cisplatin-induced AKI. Conclusion: L-carnitine resolves the cisplatin-induced urinary concentration defect, which may occur by increasing AVP/cyclic adenosine monophosphate/AQP2 levels, indicating the potential use of L-carnitine to ameliorate the renal urinary concentration effect in cancer patients treated with cisplatin.

Surgical decompression and conservative treatment are routinely used in the treatment of patients with malignant infarction of the middle cerebral artery (MIMCA). However, efficacy and safety are controversial. The purpose of this study is to systematically compare the clinical outcomes between surgical decompression and conservative treatment in patients with MIMCA.This study retrieved available academic randomized controlled trials (RCTs) comparing the clinical outcomes between surgical decompression and conservative treatment in patients with MIMCA from the databases of ScienceDirect, Cochrane Library, Embase, and PubMed. The references of previous reviews and related clinical studies were manually checked to retrieve potential literature that was not involved in our electronic search results.A total of 7 RCTs were included in the study. The overall number of participants in the surgical decompression group was 165, whereas it was 173 in the conservative treatment group. The results revealed that the surgical decompression group was related to a lower incidence of 1-year death (odds ratio [OR], 0.192; 95% confidence interval [CI], 0.119-0.309; P0.001), 1-year death in patients60 years of age (OR, 0.202; 95% CI, 0.097-0.421; P0.001), 1-year death in patients60 years of age (OR, 0.145; 95% CI, 0.069-0.301; P0.001), 1-year death in patients treated within 48 hours of stroke onset (OR, 0.159; 95% CI, 0.090-0.282; P0.001), and modified Rankin Scale (mRS) score ≤3 (OR, 2.082; 95% CI, 1.185-3.658; P0.001).Based on current evidence for patients with MIMCA, decompressive surgery not only is a life-saving therapy but also reduces the incidence of mortality without increasing the risk of severe disability.

Objective To reproduce a model of gentamicin induced apoptosis of human kidney epithelial cell (HK2), to observe the expression of death-associated protein 5 (DAP5) in HK2 cells, and to explore the relationship between PI3K/Akt/ mTOR signal pathway and DAP5. Methods HK2 cells were cultured with 3.2mg/ml gentamicin. Cell apoptosis was determined by DNA agarose gel electrophoresis, the apoptotic rate was determined with flow cytometry. Protein expressions of DAP5, Akt and p-Akt were assessed with Western blotting. Rapamycin was used to block PI3K/Akt/mTOR signal pathway in HK2 cell apoptosis model, and the change in DAP5 expression was observed. Results HK2 apoptosis appeared 24h after gentamicin treatment, and the apoptotic rate was found to be increased with the prolongation of gentamicin treatment. The apoptotic rates were 5.9%, 23.0% and 49.9%, respectively, at 24h, 36h and 72h after gentamicin treatment (P

Considering the characteristics and control requirements of assembly line, we develop a virtual simulation system which combines virtual reality and electrical control technology. The system can be used in system monitoring, debugging and staff training. The electrical control program is written by software of Rockwell based on virtual PLC, which realizes the application of velocity curve in linear type and Cosine-linear type on the object of virtual simulation through the RSTestStand software to build 3D virtual simulation model of the automatic assembly system of manipulator. The motion visual effect and monitor curve on the object of virtual simulation can indicate that the control system works well. It can also reduce mechanical arm joint motion and force effectively, through the start and stop, acceleration and deceleration of the manipulator movement and optimization of the speed using cosine timing scheme in uniform conversion.

In this paper, a multi-disciplinary optimization frame considering weld fatigue constraints is constructed based on kriging surrogate model in order to improve the fatigue reliability of vehicle structures. Under this frame, kriging surrogate model is used to construct weight, strength, stiffness, frequency, fatigue life responses for optimization, which improves effectively the computing efficiency. For weld fatigue analysis, master S-N curve method in AMSE criterion is used for reliable fatigue life. The curb girders of a high-speed train are taken as an example, a light-weight optimization model considering static strengthen and weld fatigue constrains is constructed and solved by use of submodelling technology under the proposed optimization frame. After optimization, the weight of the curb girders is reduced about 33% from 1.73 ton to 1.16 ton. These results show the proposed optimization method is effective, and provide the preference for the light-weight design of high-speed trains.

Because traditional keyboard quality manual inspection is inefficient and low reliability for subjectivity, machine vision based automated inspection system was developed. Keyboards fine difference in position and rotation angles existing in captured images influenced image matching. Hough transform was applied to detect the edges of keyboards for their image calibration. Many small image templates for each key and a global template for entire keyboard were set up for automated inspecting character printing quality with moderate difference sufferance. Two CCD cameras were used for image capturing to deal with keyboard big area and software was developed on HexSight Image Kit. Experimental results showed that keyboard quality can be effectively inspected with better performance.

In SMT solder paste deposit 3D measurement based on PMP, conventional phase unwrapping method suffered from shadow, noise and holes. Considering practical engineering condition, phase unwrapping method can be improved using 2D image information: basic principle method was applied to smooth area, quality-guided unwrapping and least-square method were taken in shadow or break area for phase unwrapping respectively. Experimental results show that desired phases can be correctly and quickly unwrapped in proposed method.

Based on the experiential data from the operation of Chongqing No.2 light rail for six years, and combined with the deep analysis for the tire wear characteristic of the straddle type monorail vehicle, this paper presents an expert system of detection and management for the tire wear of straddle type monorail vehicle. Firstly, this paper will analyze the wear characteristic of the straddle type monorail vehicle tire. Secondly, this paper will give the basic design idea of the expert system. And finally the design method of the expert system will be presented with the use of access software and visual basic language. Practices show that the expert system presented in this paper can meet the actual need of detection and management for the tire wear of the straddle type monorail vehicle, and is effective for reducing repair cost and increasing the economic benefit for operation of urban railway traffic.

This paper mainly uses image pre-processing and feature extraction to calculate the invariant moment of image, and ultimately realizes the image pattern recognition based on ART-2 neural network. Experimental results show that ART-2 neural network has high recognition rate. It also solves the contradiction between network's plasticity and stability, when new recognition model appears.

The surface brightness values in different directions are different, the measurement of the existing optical gloss meter only once can not simultaneously achieve measurement from different directions, for this phenomenon using orthogonal pairs of optical measurement method, two groups of measurements should be averaged. Measuring probe is made of a 2kHz pulse modulation circuit, silicon photovoltaic cells, I / V conversion, high-pass filter, such as precision rectifier circuit. Measurement probe output signal by the dual analog switch and the ADC into the computer. System software using VB, can realize to show the average computation, numerical analysis and results of statistical functions. Semiconductor laser light source eliminates the need of optical collimating lens. Measurement of laser modulation frequency is 2kHz, much higher than the environmental stray light, using techniques based on frequency domain interference with satisfactory light scattering resisting effect,the test shows that the error caused by stray light is less than 1.5% ,when the instrument works open-ended in the light path.

Human Skin Color(HSC) features have been widely used in video moving human positioning. However, in complex background video sequences, due to illumination changes or other moving objects which have similar HSC regions, the effect of moving human positioning is not satisfactory. A new method of moving human positioning applied on complex background video sequences is presented in this paper. Firstly, brightness information of the video sequence images is detected and analyzed based on HSV color model. Secondly, adopt the multi frame subtraction method to extract the moving object regions from motionless background. Then, the regions with distinctive HSC features are separated from other moving objects using the data fusion model of HSC and brightness information. Finally, identify human object among regions with HSC features according to the prior knowledge of human. The experimental results show that the method provided in this paper is effective in moving human positioning of complex background video, and has the strong illumination change adaptability and anti-jamming ability.

The LBAs (Lewis acid-assisted Bronsted acid catalysis) is proposed as possible mechanistic process in the simple FeCl3-catalyzed Michael reactions of chalcones with active methylene compounds in organic solvents. And iron salts were found to be effective promoters in the asymmetric Michael addition of 4-hydroxycoumarin to α,β-unsaturated ketone, which resulted in excellent yield and high level of enantioselectivity (up to 91% ee) in the presence of low catalytic amount of iron and simple chiral primary amine.

Highly efficient hydroamination reactions of sulfonamides, carboxamides, and carbamates with unactivated olefins catalyzed by simple and inexpensive zirconium salts under mild reaction conditions were presented for the practical preparation of various amines. These processes gave good to excellent yields of the addition products in Markovnikov addition fashion.

Iron–palladium is a superior bimetallic catalyst in the presence of acetylacetone (Acac) with remarkable synergistic effect for the Michael-type Friedel–Crafts reactions of indoles with chalcones. This catalytic system has the advantages of mild reaction conditions, smaller amount of metal salts, high yields of the desired products and operational simplicity, which make it a useful and promising process for the synthesis of indole derivatives. Copyright © 2009 John Wiley & Sons, Ltd.

A 69-year-old man, who had been dialyzed using a permanent central venous catheter for 2 years, presented with Henoch-Schonlein purpura and positive perinuclear anti-neutrophil cytoplasmic antibody (p-ANCA). He was diagnosed with catheter-related infection by Staphylococcus aureus. After administration of antibiotic and steroid therapy, purpura disappeared and p-ANCA gradually became negative. This case supports the conclusion that infection can be pathogenesis of the vasculitis, including ANCA-positive HSP. Additionally, impregnation of catheters with antibiotics can be an effective treatment for catheter infections.

In this article, we present the case of a man with uremia. Laboratory testing revealed thrombocytopenia, erythrocyte fragmentation, elevated lactate dehydrogenase, and malignant hypertension, manifestations that are similar to thrombotic microangiopathy (TMA). Thromboasthenia, manifested as a decrease in the platelet aggregation rate, was also noted. Regular hemodialysis (3 times per week) improved the patient’s thrombocytopenia and thromboasthenia. This case supports the conclusion that uremic toxin, which can be removed by hemodialysis, inhibits the quantity and quality of platelets. We believe that the platelet aggregation rate can be a useful tool in distinguishing uremia from TMA.

In machine vision inspection with area array camera, inspecting windows placement needs to be optimized. It aims to minimize the number of windows while covering whole inspected objects. A knowledge inductive search approach is proposed in this work to randomly search optimal window positions with partly prior knowledge direction and partly posteriori knowledge induction. Experimental results show that this approach uses the least number of windows.

The reaction of 1,3-dicarbonyl compounds with chalcones is investigated in the presence of FeCl3.

The reliability and distance of Power Line Communication (PLC) are the most important factors, which limit the application of PLC in practice seriously. This paper discusses the specialties and the methods of building up the PLC networks, uses an improved Dynamic Ant Colony System algorithm (DACS) in dynamic routing of PLC networks, and the multi-path storage technology is given. The improved DACS enhanced the accuracy and efficiency of dynamic routing in two aspects, that is the best tours and the worst tours. A dynamic penalty on worst tours will open up chances for ants to navigate, limit intentions and provide cautions in an ant's decision to move. And ants can get better search guidance by concentrating only the best tours from all groups. The simulation and experimentation indicate that the improved DACS provides an effective dynamic routing method for the expansion of PLC. It is an effective method of improving the reliability of PLC.

Iron–palladium is a superior bimetallic catalyst in the presence of acetylacetone (Acac) with remarkable synergistic effect for the Michael-type Friedel–Crafts reactions of indoles with chalcones. This catalytic system has the advantages of mild reaction conditions, smaller amount of metal salts, high yields of the desired products and operational simplicity, which make it a useful and promising process for the synthesis of indole derivatives. Copyright © 2009 John Wiley & Sons, Ltd.

Hemagglutinin gene of subtype H5 avian influenza virus was amplified by polymerase chain reaction to construct expression cassette containing FPV early, late promoter and SV40 polyA tail. Then delivery vector was constructed by subcloning hemagglutinin gene of subtype H5 and GFP gene into fowlpox virus recombinant arm. The delivery vector and Lipid were transfected into CEF cells preinfected with FPV 282E4 strain virus. Recombinant fowlpox virus expressing the green fluorescence protein and hemagglutinin gene was screened and plaques were purified in CEF cell. After a second cotransfection with Cre recombinase plasmid, a recombinant virus only including hemagglutinin gene was gained. The immunofluorescent assay and replication efficiency of virus proved the recombinant could replicate steadily and express subtype H5 hemagglutinin gene. Two groups of 8-day-old SPF chickens were vaccinated with rFPVH5 by the wing-web method at the dosage of 10(5) PFU and 2 x 10(5) PFU respectively. After 28 days,antibodies titer was tested by HI. The results showed that the recombinant fowlpox virus could activate high antibody response.

Water containing Oscillatoria agardii was cultured under controlled conditions and harvested in the late log growth phase. The objective was to determine: the contribution of algae cells and algae extracellular organic matter (EOM) to the disinfection by-products formation potential (DBPFP), and the effects of preozonation including ozone dosage and preozonation time on DBPFP of algae cells and EOM and mechanism of these effects. The results show that the main trihalomethanes from both Oscillatoria cells and EOM are chloroform and bromodichloromethane, and that the main haloacetic acids are dichloroacetic acid and trichloroacetic acid. HAAFP from algae cells and EOM themselves or after preozonation followed by coagulation is more than THMFP, which shows that more attention should be paid to the control of HAA in the treatment of algae containing water. DBPFP of EOM is reduced by preozonation, and DBPFP clearly decreases with time. Compared with traditional coagulation, the dosage of 0.975 mg/L preozonation with reaction time of 10 minntes followed by coagulation can decrease DBPFP of EOM by 31% and decrease HAAFP by 52.6%, but increase THMFP by 12.5% under this experiment's condition. This result shows the major reason preozonation can control the DBPFP of EOM is that it can control HAAFP effectively. At the same time, the DBPFP of algae cells is significantly increased by preozonation, and this increase is almost a linear function of preozonation time. It can be concluded that in the real water treatment case most of algae cells should be removed intact before preozonation to control the DBPFP.

To establish the diagnostic criteria of intima-media thickening (IMT) of the carotid artery, femoral artery and thoracic aorta in normal subjects and evaluate the effects of drugs on IMT in patients with atherosclerosis with high-frequency ultrasound imaging.259 normal subjects and 102 patients with atherosclerosis (AS) were studied. 134 normal subjects were examined by transesophegeal echocardiography. 102 patients with AS were randomly divided into 4 groups:simvastatin group, probucol group, captopril group, and placebo group. The values of IMT, including IMT of the normal segment (IMT(A)) and the maximal IMT of each artery (IMT(B)) of the carotid and femoral arteries, and thoracic aorta, the mean maximal IMT of the 4 arteries (IMT(C)), and the single maximal IMT among the 4 arteries (IMT(D)) were measured. The examinations were repeated in 3 years after treatments.In carotid, and femoral arteries and thoracic aorta, IMT increased with aging. The IMT values of the carotid and femoral arteries and thoracic aorta were 0.63 mm +/- 0.15 mm, 0.68 mm +/- 0.21 mm, 1.02 mm +/- 0.22 mm respectively with the upper limits of 0.93 mm, 1.10 mm, and 1.46 mm respectively. IMT(A) increased 0.023 mm per year. The progression value of IMT(D) in all therapeutic groups were significantly lower than that of the control group (all P0.05).It is suggested that the IMT0.93 mm,1.10 mm, and1.46 mm is be defined as indicators of IMT in carotid and femoral arteries and thoracic aorta respectively. Drug therapy can decrease the progression of atherosclerosis but has no effects on the IMT in normal segment of arteries. High frequency ultrasound technique is reliable in monitoring the progression or regression of atherosclerosis in peripheral arteries.

Interaction between cytotoxic T lymphocyte-associated antigen-4 (CTLA4, CD152) and B7 molecules (B7-1 and B7-2) is of importance in the cellular events of lymphocyte, including antigen-specific T-cell activation and induction of autoreactive T-cell. We describe here the first introduction of a murine soluble CTLA4 gene, CTLA4Ig, to Mm1 cells, a macrophagic cell line. CTLA4Ig was successfully expressed on Mml cells and the expressed CTLA4Ig was found to be functionally active in their binding to B7 molecules by flow cytometry and immunofluorescence studies. The biological activity of CTLA4Ig from the transfected Mm1 cells was studied and showed inhibitory activity on mixed lymphocyte culture. A high CTLA4Ig producing macrophagic cell line was obtained. As Mm1 cells were regarded as difficult for gene transfection and there had so far been no report on expression of CTLA4Ig gene on Mm1 cells, these results suggested that the CTLA4Ig expressing Mm1 cells could be useful for analysis of CTLA4 and B7 molecule interaction in both macrophage and T-cell.