Search

A novel missense variant (NM_005327.7: c.99CG, p.Ile33Met) was discovered in 3-hydroxyacyl-CoA dehydrogenase (HADH), which is involved in congenital hyperinsulinism (CHI). This variant may be damaging or deleterious, as assessed using protein prediction software. This study aimed at the impact of this variant on islets and if it caused the leu-sensitive insulin secretion. The adenoassociated virus containing the HADH missense variant (p.Ile33Met), wild-type (WT) HADH or empty vector (EV) was constructed, and the rats were infected with it. Three weeks after the transfection, 15 rats were dissected to observe the effect of the variant on the islet tissue. Then we treated the remaining rats with leucine or sodium carboxymethyl cellulose (CMC-Na) by gavage and drew blood from the rat tail vein to detect the variations in blood glucose, serum insulin and serum glucagon. Further, we dissected the rats to observe the fluctuation of insulin and glucagon contents in pancreatic islets under the combined action of leucine and p.Ile33Met. Insulin and glucagon were observed in the islet tissue under an inverted fluorescence microscope, serum insulin and glucagon were detected by ELISA, and the blood glucose value was determined using a Roche glucometer. The positive area and average gray value of islet fluorescence pictures were analysed using the software Image J (USA). Rats expressing p.Ile33Met showed significantly higher insulin and glucagon content, as well as the islet area, compared to WT and EV rats. Moreover, after intragastric administration of leucine, the serum insulin content of the variant rats increased but the blood sugar level decreased significantly. Meanwhile, there was an appreciable decrease in the insulin content in rat pancreatic islet tissues. Our results suggest that the variant NM_005327.7: c.99CG promotes the proliferation of pancreatic islets, enhances the secretion of insulin, and induces leu-sensitive hyperinsulinaemia.

Objective To investigate the potentiality of D3-creatine dilution method to be used in detection of skeletal muscle mass, so as to provide a new method for skeletal muscle mass detection in mice. Methods Four weeks, 10 weeks and 13 months old male C57BL/6 mice (10 each) were used in the present study. All mice were fed with a single dose of D3-creatine (2mg/kg) after testing body composition by EchoMRITM. Urine was collected at 24h, 48h and 72h after creatine administration, and analyzed by liquid chromatography-tandem mass spectrometry mass spectrometry (LC-MS/MS) that was used to determine the creatine pool size. The relationship between creatine pool size and the skeletal muscle mass determined by EchoMRITM was analyzed. Results Both lean body mass and total creatine pool size were aged mice > adult mice > juvenile mice. There was a significant correlation (r=0.687, P=0.000) between the lean body mass measured by EchoMRITM and the total creatine pool size calculated by D3-creatine dilution method. Conclusion The D3-creatine dilution method may be used to determine the skeletal muscle mass of mice. DOI: 10.11855/j.issn.0577-7402.2017.07.10

For the large number of sampling points and high requirement of stabilization in ultrasonic testing,the dynamic performance of 5-dof manipulator was taken into consider: Firstly, the D-H notation and Newton-Euler were applied to set up the kinematic and dynamic equation for trajectory planning. Then the 4-3-4 polynomial which is continuous in displacement, velocity and acceleration, was used for continuous trajectory planning in joints space. Finally the trajectory based on time optimization with the constraint of velocity and acceleration including torque of actuator was validated.

With the rapid development of computer technology and wireless network communication technology, video encoding technology will be more and more widely applied in the limited resources of the wireless network. Due to the large amount of data of the video transmission , transmission quality of transmit video on the wireless network are varied with different compression parameters, network parameters and network conditions. Simulation results show that the transmission of video over wireless networks, must be based on the current network conditions, choosing the suitable GOP length and quantitative parameters to get the high image quality. In specific applications, network topology, network bandwidth, routing technology and transmission of packet segmentation scheme and other factors will affect the quality of service for video services.

Yabuki & Matsu'ura's inversion method determines fault slip distribution from geodetic data utilizing the maximum likelihood method with Akaike's Bayesian Information Criterion (ABIC) under the smoothness constraint. We use this method to estimate the co-seismic slip distribution of the 2011 Tohoku-Oki Mw9.0 earthquake on a curved fault from GPS observation data. The result indicates that the maximum slip is 35 m, strong slips are distributed in depths shallower than 30 km above the earthquake focus, and the biggest ruptures are concentrated at a depth about 20 km. The seismic moment is about 3.63×1022 N·m, corresponding to a moment magnitude Mw9.0. Our simulation results confirm that the Yabuki & Matsu'ura's inversion method is suitable for faults with dip angle less than 40°. Finally, we compute the far-field deformations in Northeast China caused by the 2011 Tohoku-Oki Mw9.0 earthquake using spherical dislocation theory of Sun et al. Our theoretical predictions agree well with the observed co-seismic displacement from China GPS Observation Network.

To test the different contrctile responses of extracellular nucleotides, such as ATP, UTP and nucleotide uridine adenosine tetraphosphate (Up4A) in gastric longitudinal muscle (LM) and circular muscle (CM). Examined the effect of P2X and P2Y receptor antagonists (in this study, we used IP5I and suramin) and cyclooxygenase inhibitor (indomethacin) on Up4A induced contractile responses in LM and CM.The rats were sacrificed and the stomachs were opened to gain LM and CM. Using organ bath system to assess contrctile responses of smooth muscle.Up4A could induce contractile responses in both CM and LM, which were similar with ATP and UTP. IP5 did not attenuate Up4A could induce contractions in both LM and CM, but suramin and indomethacin significantly inhibited Up4A contraction in CM, but not in LM.Our results suggest that extracellular nucleosides and their inhibitors induce different responses between LM and CM.

To study the pathologic feature of sudden cardiac death in Yunnan province and to investigate the role of myocarditis.During the period from 1991 to 2006, there were 29 cases of sudden cardiac death with autopsy performed. Fourteen of these cases were diagnosed to have myocarditis based on Dallas criteria and World Heart Federation's consensus. The clinical and pathologic findings were reviewed. The cardiac conduction system was examined in details by serial sectioning in 3 cases.Fourteen cases suffered with myocarditis, which accounted for 48% of all cases of sudden cardiac death studied. The age of the deceased ranged from 8 to 68 years (mean = 30 years), with male-to-female ratio equaled to 9:5. Lymphocytic myocarditis and neutrophil myocarditis were the two major types, affecting 11 and 3 cases, respectively. The inflammatory infiltrates were often patchy rather than diffuse. The inflammatory foci were detected only in 8% to 42% (average = 20%) of the paraffin sections of the heart tissue. These lesions were usually located in the lateral wall of left ventricle and occasionally in interventricular septum and right ventricular wall. Myocardial injury was mild in most cases while patchy myocytolysis or coagulation necrosis was observed only in a few cases. Most of the lesions were relatively new and histologic evidence of myocardial repairing sometimes coexisted. Pericarditis and subacute endocarditis were also identified in 4 and 1 cases, respectively. Atrioventricular node was involved by myocarditis in 1 of the 3 cases examined for cardiac conduction system. Two cases showed gross evidence of cardiac dilatation (either left ventricle or biventricular). Respiratory tract and pulmonary infection was present in 5 cases.Myocarditis represents one of the major pathologic changes of sudden cardiac death occurring in Yunnan province. The inflammation is usually focal. Further studies are required for delineation of possible etiologies which may include virus, bacteria or exogenous toxin.

To understand the epidemiologic and clinical characteristics of the "Yunnan unknown-cause sudden cardiac death (YUSCD)".Cases of YUSCD occurred in 2002-2004 were investigated with cross-sectional study. Clinical manifestation was observed and analyzed.The YUSCD areas were mountainous and most of the YUSCD cases appeared between June and August. Most of the YUSCD cases were young farmers and showed family/village clustering nature. Most of the patients died quickly with only few recovered. The overall incidence of YUSCD was 1.83% with mortality as 1.51%. The fatality rate of YUSCD appeared to be 78.1%.The YUSCD cases had a clustering feature along with time and area. The clinical manifestation of YUSCD could not be specifically identified, making the clinical diagnosis and treatment difficult when practicing in the fields that called for further studies on epidemiology, clinical work and etiology.

To identify the epidemiological and clinical features of unexpected sudden cardiac deaths (SUD) in Yunnan.Choosing the old SUD cases from Xiangyun, Heqing, Nanjian and Dayao counties and using the standardized verbal autopsy Form, we interviewed the family members of the cases, witnesses and doctors as well as reviewing their medical files to get relative information.We identified 116 SUDs in 21 villages from 1984 to 2004. The village-specific annually standardized incidence rates were ranged from 0.2/1000 to 8.9/1000 (median = 0.8/1000). 66% and 29% of the SUDs occurred in July and August respectively. The incidence rates of SUD were higher (1.6/1000, chi(2) = 16, P0.01) in 10 - 39 year-olds, and higher in females than in males (RR = 1.6, 95% CI: 1.1 - 2.3). Seventy percent of SUD occurred in families having clustering nature and 60% of the additional cases in the family were occurred within 24 hours (median = 20 hours) after the first SUD identified in the family. SUD occurred in 23 families followed the first affected family in a village during the same season. In these 23 families, 61% of the first SUD occurred within 8 days after the first SUD in the first affected family. 68% and 66% of the SUDs did not have any complaints or signs during the last 3 weeks or from 3 weeks to 2 days prior to the onset of the disease. 63% of the SUDs had cardiac symptoms within the last 2 days prior to the onset with major symptoms as dizziness, nausea, faintness, unconsciousness, weakness and palpitation. The median duration from acute onset to death was 2 hours.The extreme time-space clustering of SUD in families and in villages suggested that the risk factors occurred in specific time and location. Familial clustered SUD cases had common exposure pattern. Sudden onset of acute cardiac symptoms often followed by sudden death. Epidemiological study on new cases was necessary to identify risk factors and to develop hypothesis for causation. In July 2005, we instituted a special SUD surveillance system for all the affected counties together with 10 counties which had no reported cases.

Vilmorin23 is an internationally used differential host variety for studies on the interactions between wheat stripe rust and wheat. It contains the stripe rust resistance gene YrV23 and is potentially an important source of stripe rust resistance worldwide. SSR analysis was performed on the wheat NIL Taichuang 29*6/YrV23 carrying the resistant gene YrV23 against stripe rust, Vilmorin 23 and its recurrent parent Taichung 29. Fifty pairs of SSR primers on wheat chromosome 2B were screened and a reproducible polymorphic DNA fragment amplified by Xwmc356 was found. Genetic linkage was tested on 150 segregating F2 plants. It showed that the microsatellite marker Xwmc356 was linked to the resistance gene YrV23 with a genetic distance of 9.4 cM.

The synthesis, crystal structures, fluorescent properties and chirality of Zn(II) coordination complexes with two kinds of salen-type Schiff based ligands are reported. One of them is a chiral molecule and exhibits an unusually intense orange–red visible fluorescent emission in the present of THF, while the other is silent in photoluminescent emission.

Two kinds of tetranuclear Zn(II) molecular clusters with the designed aroylhydrazone Schiff base ligand (H3333L), [Zn4(HL)4]·3(DMF)·3(H2O) (1) and [Zn4(HL)2(CH3COO)4]·2(DMF)·2(CH3OH) (2), have been synthesized and studied. Molecular cluster 1 displayed a Zn4O4 boat-shaped core and 2 presented a discrete linear tetranuclear Zn(II) structure. The luminescent properties of the two molecular clusters have been investigated, which exhibit a wide range of photo-luminescence in different organic solvents.

INTRODUCTION: Since the late 1970's, time-space clusters of sudden unexplained death (SUD) in northwest Yunnan, China have alarmed the public and health authorities. From 2006-2009, we initiated enhanced surveillance for SUD to identify a cause, and we warned villagers to avoid eating unfamiliar mushrooms. METHODS: We established surveillance for SUD, defined as follows: sudden onset of serious, unexplained physical impairment followed by death in

To investigate the clinical features of unexpected sudden death (SUD) clustered in families in Yunnan province.This retrospective study analyzed the clinical features of SUD occurred between July to September 2005 in 7 families in Yunnan province.All 16 SUD patients shared common clinical features such as fatigue and repeated syncope and one group of SUD patients (n = 8 from 4 families) presented with the gastric intestinal tract manifestations including nausea, vomiting, abdominal pain and diarrhea with suspected dietary history and abnormal laboratory enzyme findings (GOT/GPT, CK/CKMB, LDH/LDH1 etc.). In SUD patients without gastric intestinal tract manifestations (n = 8 from 3 families), there were no clear symptoms before death and repeated ventricular tachycardia and ventricular fibrillation were recorded in one survivor. There was no clear evidence for the involvements of hereditary and infectious factors for observed SUD.The reason for the unexpected sudden death clustered in 7 families in Yunnan remains unclear. Repeated syncope and fatigue served as the common clinical features in the presence or absence of gastric intestinal tract manifestations in all SUD cases. Further studies are needed to clarify the pathology and detailed clinical manifestations of SUD occurred in this area.