1. Phenotype and function of monocyte-derived dendritic cells in neonates born to Hepatitis B virus-positive mothers
- Author
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Yong mei Shen, Xiao hong Shi, Xiao yan Yan, Y L Feng, Jian Guo, Su ping Wang, Xiu juan Yang, Bo Wang, Yi Gao, Yue se Yuan, Yu lan Qiu, Jun ni Wei, and Yan Hao
- Subjects
Hepatitis B virus ,CD86 ,medicine.diagnostic_test ,T cell ,Biology ,medicine.disease_cause ,Mixed lymphocyte reaction ,Phenotype ,Flow cytometry ,medicine.anatomical_structure ,Immunology ,medicine ,CD80 ,Function (biology) - Abstract
Hepatitis B Virus (HBV) infection in infancy or early childhood leads to high rate of persistent infection (25-90%). The immunological basis of high rate of viral persistence in vertically acquired HBV infections is not completely understood. Dendritic cells (DCs) are one of the most potent antigen-presenting cells (APC) and play pivotal roles in the enhancement or regulation of antiviral immune reactions. Aim of the present study was to investigate whether an HBV-infected maternal environment might influence the infants’ DC phenotype and function. Monocyte-derived DC (MoDC) of neonates born to HBsAg-positive mothers were studied phenotypically by Flow Cytometry (FCM) and functionally by mixed lymphocyte reaction (MLR) and enzyme-linked immunosorbent assay (ELISA). An electron microscope was used to analyze the morphological changes of MoDC. MoDC from neonates whose maternal HBV DNA was>5×107 copies/ml showed a reduced surface expression of CD80, CD86, and HLA-DR as compared to that in neonates whose maternal HBV DNA was negative (CD80: t=3.238, P=0.002; CD86: t=3.543, P=0.001; HLA-DR: t=2.785, P=0.008). T-cell proliferation assays also showed an impaired allostimulatory capacity in comparison to that in neonates whose maternal HBV DNA was negative, especially in the cultures at a DC: T cell ratios of 1:5 and 1:10 (t=-5.442, P
- Published
- 2020