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1. Multi-stage progressive detection method for water deficit detection in vertical greenery plants

Author

Fei Deng, Xuan Liu, Peng Zhou, Jianglin Shen, and Yuanxiang Huang

Subjects
Vertical greening plant, Deep learning, Object detection, Multi-stage, Swin transformer, Self-attention, Medicine, Science
Abstract

Abstract Detecting the water deficit status of vertical greenery plants rapidly and accurately is a significant challenge in the process of cultivating and planting greenery plants. Currently, the mainstream method involves utilizing a single target detection algorithm for this task. However, in complex real-world scenarios, the accuracy of detection is influenced by factors such as image quality and background environment. Therefore, we propose a multi-stage progressive detection method aimed at enhancing detection accuracy by gradually filtering, processing, and detecting images through a multi-stage architecture. Additionally, to reduce the additional computational load brought by multiple stages and improve overall detection efficiency, we introduce a Swin Transformer based on mobile windows and hierarchical representations for feature extraction, along with global feature modeling through a self-attention mechanism. The experimental results demonstrate that our multi-stage detection approach achieves high accuracy in vertical greenery plants detection tasks, with an average precision of 93.5%. This represents an improvement of 19.2%, 17.3%, 13.8%, and 9.2% compared to Mask R-CNN (74.3%), YOLOv7 (76.2%), DETR (79.7%), and Deformable DETR (84.3%), respectively.

Published
2024
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2. A machine learning model based on clinical features and ultrasound radiomics features for pancreatic tumor classification

Author

Shunhan Yao, Dunwei Yao, Yuanxiang Huang, Shanyu Qin, and Qingfeng Chen

Subjects
pancreatic tumors, malignant, clinical features, radiomics features, machine learning, classification nomogram, Diseases of the endocrine glands. Clinical endocrinology, RC648-665
Abstract

ObjectiveThis study aimed to construct a machine learning model using clinical variables and ultrasound radiomics features for the prediction of the benign or malignant nature of pancreatic tumors.Methods242 pancreatic tumor patients who were hospitalized at the First Affiliated Hospital of Guangxi Medical University between January 2020 and June 2023 were included in this retrospective study. The patients were randomly divided into a training cohort (n=169) and a test cohort (n=73). We collected 28 clinical features from the patients. Concurrently, 306 radiomics features were extracted from the ultrasound images of the patients’ tumors. Initially, a clinical model was constructed using the logistic regression algorithm. Subsequently, radiomics models were built using SVM, random forest, XGBoost, and KNN algorithms. Finally, we combined clinical features with a new feature RAD prob calculated by applying radiomics model to construct a fusion model, and developed a nomogram based on the fusion model.ResultsThe performance of the fusion model surpassed that of both the clinical and radiomics models. In the training cohort, the fusion model achieved an AUC of 0.978 (95% CI: 0.96–0.99) during 5-fold cross-validation and an AUC of 0.925 (95% CI: 0.86–0.98) in the test cohort. Calibration curve and decision curve analyses demonstrated that the nomogram constructed from the fusion model has high accuracy and clinical utility.ConclusionThe fusion model containing clinical and ultrasound radiomics features showed excellent performance in predicting the benign or malignant nature of pancreatic tumors.

Published
2024
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3. Application of Split Residual Multilevel Attention Network in Speaker Recognition

Author

Jiji Wang, Fei Deng, Lihong Deng, Ping Gao, and Yuanxiang Huang

Subjects
Attention mechanisms, DTFA, speaker identification, split-ResNet, Electrical engineering. Electronics. Nuclear engineering, TK1-9971
Abstract

Current speaker recognition systems are mainly for the combined application of network architectures and attention mechanisms, however, lightweight networks are not able to extract frame-level features of speaker speech well, and deeper and wider networks also face the problems of slower inference and excessive number of parameters. To this end, we proposes Split-ResNet, a network structure for split residuals, which can obtain a combination of multiple receptive field at a finer-grained level, thus obtaining a variety of feature representations with different scale combinations and producing more informative and comprehensive multi-scale features. In addition we propose a dual time-frequency attention (DTFA) that enhances key features and suppresses unimportant features by focusing on features in the time and frequency domains and learning weights from the time and frequency channels, respectively. We finally tested the speaker recognition system using a combination of Split-ResNet and DTFA against other speaker recognition systems on the Voxceleb1-O test set. The test results show that the speaker recognition system proposed in this paper is 0.98%, 0.39%, 0.69% and 0.47% lower in EER compared with SpeechNAS, RawNet2, Y-vector and CNN+Transformer, respectively, proving that DTFA+Split-ResNet is a speaker recognition system with good speaker audio feature extraction capability and discriminative capability.

Published
2023
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4. Identification and characterization of heat-responsive lncRNAs in maize inbred line CM1

Author

Xiaolin Hu, Qiye Wei, Hongying Wu, Yuanxiang Huang, Xiaojian Peng, Guomin Han, Qing Ma, and Yang Zhao

Subjects
Maize, Heat stress, LncRNA, Target genes, Regulatory network, Biotechnology, TP248.13-248.65, Genetics, QH426-470
Abstract

Abstract Background Frequent occurrence of extreme high temperature is a major threat to crop production. Increasing evidence demonstrates that long non-coding RNAs (lncRNAs) have important biological functions in the regulation of the response to heat stress. However, the regulatory mechanism of lncRNAs involved in heat response requires further exploration and the regulatory network remains poorly understood in maize. Results In this research, high-throughput sequencing was adopted to systematically identify lncRNAs in maize inbred line CM1. In total, 53,249 lncRNAs (259 known lncRNAs and 52,990 novel lncRNAs) were detected, of which 993 lncRNAs showed significantly differential expression (DElncRNAs) under heat stress. By predicting the target genes, 953 common targets shared by cis- and trans-regulation of the DElncRNAs were identified, which exhibited differential expression between the control and the heat stress treatments. Functional annotation indicated that a number of important biological processes and pathways, including photosynthesis, metabolism, translation, stress response, hormone signal transduction, and spliceosome, were enriched for the common targets, suggesting that they play important roles in heat response. A lncRNA-mediated regulatory network was constructed to visualize the molecular response mechanism in response to heat stress, which represented the direct regulatory relationships of DElncRNAs, differentially expressed miRNAs, target genes, and functional annotations. Conclusions This study lays a foundation for further elucidation of the regulatory mechanism for the response to heat stress in the maize inbred line CM1. The findings provide important information for identification of heat-responsive genes, which will be beneficial for the molecular breeding in the cultivation of heat-tolerant maize germplasm.

Published
2022
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5. Edaravone Dexborneol Downregulates Neutrophil Extracellular Trap Expression and Ameliorates Blood-Brain Barrier Permeability in Acute Ischemic Stroke

Author

Yuanxiang Huang, Xiangjian Zhang, Cong Zhang, Wenting Xu, Wenshuo Li, Zixin Feng, Xianglan Zhang, and Keke Zhao

Subjects
Pathology, RB1-214
Abstract

Background. Our previous work has shown that inflammatory processes play a detrimental role in the pathophysiology of acute ischemic stroke (AIS). Neutrophil extracellular traps (NETs) have been recognized as a key contributor to the proinflammatory response in AIS and could aggravate blood-brain barrier (BBB) damage. Recently, experimental and clinical researches showed that Edaravone Dexborneol (Eda.B), which is comprised of two active ingredients, Edaravone and (+)-Borneol, was effective in treatment of AIS. However, it is not clear whether the effects of Eda.B against AIS are related to NETs and BBB permeability. Methods. Experiment 1 was to detect the effects of Eda.B in AIS patients. Serum samples of volunteers and AIS patients were collected before and 3 days after Edaravone Dexborneol treatment. Markers of NETs and occludin were detected by ELISA kit. Experiment 2 was to explore the effects of Eda.B on experimental stroke mice. Male C57BL/6 mice were subjected to distal middle cerebral artery occlusion (MCAO) and treated with vehicle, Eda.B, or DeoxyribonueleaseI (DNase I). After stroke, the neurobehavioral tests, infarct volume, and cerebral blood flow evaluation were determined. Leakage of Evans blue was to assess the integrity of BBB. Western blot, real-time quantitative polymerase chain reaction (RT-qPCR), and immunofluorescence were used to examine the expression of NETs and tight junction- (TJ-) associated proteins. Results. Eda.B significantly improved neurological function and cerebral blood flow but reduced infarct volume after experimental stroke. Eda.B downregulated level of NETs in serum samples of AIS patients and tissue samples of MCAO mouse cortex. Eda.B and DNase I alleviated BBB permeability by upregulating TJ-associated proteins. Conclusion. NETs are related to the early stage of AIS. Eda.B exerted neuroprotective effects and ameliorated BBB permeability after AIS.

Published
2022
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6. Identification and Characterization of the Core Region of ZmDi19-5 Promoter Activity and Its Upstream Regulatory Proteins

Author

Yang Zhao, Lijuan Xu, Yuanxiang Huang, Hongying Wu, Xingen Zhang, Xiaolin Hu, and Qing Ma

Subjects
maize, ZmDi19-5, drought stress, promoter, regulatory proteins, Biology (General), QH301-705.5, Chemistry, QD1-999
Abstract

Drought-induced 19 (Di19) family genes play important roles in plant growth, development, and environmental stress responses. However, little is known about this family in maize. The upstream regulatory network of Di19 genes remains poorly understood in plant stress response, especially. In this study, seven ZmDi19 genes were identified, and sequence alignment, gene structure, and phylogenetic analysis was conducted. According to the phylogenetic analysis, the ZmDi19-5 promoter was cloned and multiple putative stress-responsive cis-acting elements (CAEs) were found in the promoter region. The transient transformation assay indicated that firefly luciferase (LUC)-expressed activity driven by the ZmDi19-5 promoter can be significantly induced by drought stress. A 450 bp core region of ZmDi19-5 promoter was identified, and 28 upstream regulatory proteins were screened using yeast one-hybird (Y1H) system. According to the functional annotation, some genes were related to photosynthesis, light response, and water transport, which may suggest the important roles of these genes in drought response. Particularly, five members that may be involved in drought response exhibited strong binding activity to the core region of the ZmDi19-5 promoter. This study laid an important foundation for further revealing the molecular mechanisms and regulatory network of Di19 genes in drought stress response.

Published
2022
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7. Comparative Transcriptomics Reveals the Molecular Mechanism of the Parental Lines of Maize Hybrid An’nong876 in Response to Salt Stress

Author

Xingen Zhang, Jing Liu, Yuanxiang Huang, Hongying Wu, Xiaolin Hu, Beijiu Cheng, Qing Ma, and Yang Zhao

Subjects
maize, An’nong876, salt stress, RNA-seq, Biology (General), QH301-705.5, Chemistry, QD1-999
Abstract

Maize (Zeamays L.) is an essential food crop worldwide, but it is highly susceptible to salt stress, especially at the seedling stage. In this study, we conducted physiological and comparative transcriptome analyses of seedlings of maize inbred lines An’nong876 paternal (cmh15) and An’nong876 maternal (CM37) under salt stress. The cmh15 seedlings were more salt-tolerant and had higher relative water content, lower electrolyte leakage, and lower malondialdehyde levels in the leaves than CM37. We identified 2559 upregulated and 1770 downregulated genes between salt-treated CM37 and the controls, and 2757 upregulated and 2634 downregulated genes between salt-treated cmh15 and the controls by RNA sequencing analysis. Gene ontology functional enrichment analysis of the differentially expressed genes showed that photosynthesis-related and oxidation-reduction processes were deeply involved in the responses of cmh15 and CM37 to salt stress. We also found differences in the hormone signaling pathway transduction and regulation patterns of transcription factors encoded by the differentially expressed genes in both cmh15 and CM37 under salt stress. Together, our findings provide insights into the molecular networks that mediate salt stress tolerance of maize at the seedling stage.

Published
2022
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8. Efficacy and safety of human urinary kallidinogenase for acute ischemic stroke: a meta-analysis

Author

Yuanxiang Huang, Binglei Wang, Yue Zhang, Peize Wang, and Xiangjian Zhang

Subjects
Medicine (General), R5-920
Abstract

Objective Human urinary kallidinogenase (HUK) is a glycoprotein extracted from human urine that is used to treat stroke by triggering positive regulation of the kallikrein–kinin system. Our aim was to evaluate the efficacy and safety of HUK treatment for acute ischemic stroke. Methods We searched the online databases PubMed, Embase, Cochrane Library, Google Scholar, and China National Knowledge Infrastructure (CNKI) for papers published between January 2015 and December 2019. The quality of each trial was assessed using the Cochrane Reviewers’ Handbook. Randomized controlled trials of HUK in patients with acute ischemic stroke were included. Results Sixteen trials with 1326 participants were included. The HUK injection groups had more neurological improvement than the control groups in National Institutes of Health Stroke Scale scores (mean difference, –1.65; 95% confidence interval [CI], –2.12 to –1.71) and clinical efficacy (1.30; 95% CI, 1.21 to 1.41). Subgroup analysis indicated that age may influence heterogeneity. Eleven trials reported adverse effects and there were no significant differences between the control and HUK groups (risk difference, 0.01; 95% CI, –0.02 to 0.04). Conclusions HUK ameliorates neurological symptoms in stroke patients with few adverse effects. Further high-quality, large-scale randomized trials are needed to confirm these results.

Published
2020
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12. Study on prediction and optimization of gas–solid erosion on S-Zorb reactor distribution plate

Author

Guofu Ou, Yuanxiang Huang, Minghao Luo, Youjie Gu, and Haozhe Jin

Subjects
Mechanical Engineering, General Materials Science, Industrial and Manufacturing Engineering
Abstract

Adsorption desulfurization of catalytic gasoline (S Zorb) is an important desulfurization measure that is performed to meet the environmental protection requirements before the final product oil is sold in the market. The desulfurization reactor is a gas–solid two-phase flow environment composed of high-temperature and high-pressure hydrogen-oil mixed gas and sorbent particles; erosion prominently occurs on the reactor distribution plate. This study selects the typical gas–solid two-phase flow conditions and defines the erosion mechanism of the gas–solid two-phase flow environment for the plastic material of E347. Moreover, an S Zorb desulfurization reactor model is constructed, the CFD-DEM model is adopted to predict the wall erosion characteristics in a gas–solid two-phase flow environment, typical erosion laws are obtained via calculations. The erosion laws under the influence of variable parameters are studied based on the orthogonal test, the orthogonal test results show the best parameter combination, the parameter combination yields the maximum erosion rate and high erosion area that are 29.9% and 17.3%, respectively, lower than the existing values. Moreover, an optimum scheme of the inner structure parameters of the reactor is determined for reducing erosion rate and area.

Published
2023

13. Testosterone enhances mitochondrial complex V function in the substantia nigra of aged male rats

Author

Hui Zhao, Yunxiao Kang, Yu Wang, Geming Shi, Yuanxiang Huang, Tianyun Zhang, Guoliang Zhang, Wensheng Yan, Li Wang, Xiaoming Ji, and Rui Cui

Subjects
Male, medicine.medical_specialty, Aging, Substantia nigra, ATP5I, mitochondrial complex V, Adenosine Triphosphate, Downregulation and upregulation, Internal medicine, ATP5C1, medicine, Animals, Orchiectomy, aged male rats, Testosterone, Chemistry, Dopaminergic Neurons, Dopaminergic, Cell Biology, Mitochondrial Proton-Translocating ATPases, Rats, Substantia Nigra, Endocrinology, ATP5L, nervous system, testosterone, Research Paper
Abstract

Deficits in coordinated motor behavior and mitochondrial complex V activity have been observed in aged males. Testosterone supplementation can improve coordinated motor behavior in aged males. We investigated the effects of testosterone supplementation on mitochondrial complex V function in the substantia nigra (a brain region that regulates motor activity) in aged male rats. These rats exhibited diminished ATP levels, attenuated mitochondrial complex V activity, and reduced expression of 3 of the 17 mitochondrial complex V subunits (ATP6, ATP8 and ATP5C1) in the substantia nigra. Testosterone supplementation increased ATP levels, mitochondrial complex V activity, and ATP6, ATP8 and ATP5C1 expression in the substantia nigra of the rats. Conversely, orchiectomy reduced mitochondrial complex V activity, downregulated ATP6 and ATP8 expression, and upregulated ATP5C1, ATP5I and ATP5L expression in the substantia nigra. Testosterone replacement reversed those effects. Thus, testosterone enhanced mitochondrial complex V function in the substantia nigra of aged male rats by upregulating ATP6 and ATP8. As potential testosterone targets, these two subunits may to some degree maintain nigrostriatal dopaminergic function in aged males.

Published
2020

14. Identification and characterization of heat-responsive lncRNAs in maize inbred line CM1

Author

Xiaolin Hu, Qiye Wei, Hongying Wu, Yuanxiang Huang, Xiaojian Peng, Guomin Han, Qing Ma, and Yang Zhao

Subjects
MicroRNAs, Genetics, High-Throughput Nucleotide Sequencing, RNA, Long Noncoding, Zea mays, Heat-Shock Response, Biotechnology
Abstract

BackgroundFrequent occurrence of extreme high temperature is a major threat to crop production. Increasing evidence demonstrates that long non-coding RNAs (lncRNAs) have important biological functions in the regulation of the response to heat stress. However, the regulatory mechanism of lncRNAs involved in heat response requires further exploration and the regulatory network remains poorly understood in maize.ResultsIn this research, high-throughput sequencing was adopted to systematically identify lncRNAs in maize inbred line CM1. In total, 53,249 lncRNAs (259 known lncRNAs and 52,990 novel lncRNAs) were detected, of which 993 lncRNAs showed significantly differential expression (DElncRNAs) under heat stress. By predicting the target genes, 953 common targets shared bycis- andtrans-regulation of the DElncRNAs were identified, which exhibited differential expression between the control and the heat stress treatments. Functional annotation indicated that a number of important biological processes and pathways, including photosynthesis, metabolism, translation, stress response, hormone signal transduction, and spliceosome, were enriched for the common targets, suggesting that they play important roles in heat response. A lncRNA-mediated regulatory network was constructed to visualize the molecular response mechanism in response to heat stress, which represented the direct regulatory relationships of DElncRNAs, differentially expressed miRNAs, target genes, and functional annotations.ConclusionsThis study lays a foundation for further elucidation of the regulatory mechanism for the response to heat stress in the maize inbred line CM1. The findings provide important information for identification of heat-responsive genes, which will be beneficial for the molecular breeding in the cultivation of heat-tolerant maize germplasm.

Published
2021

15. [Progress in analysis methods for major mycotoxins in foodstuffs]

Author

Fengqi, Wu, Zhenfeng, Yue, Yi, Zhang, Yuanxiang, Huang, and Jinglan, Wen

Subjects
Aflatoxins, Food Contamination, Mycotoxins, Ochratoxins, Food Analysis
Abstract

The analysis of mycotoxins in foodstuffs is affected by the complexity of the matrix and the extremely low concentration levels. The development of sample pretreatment and analytical methods that enable highly selective enrichment as well as highly sensitive detection is of great significance for food safety. This paper reviews the recent progress in biotoxin analysis methods and summarizes the prospects and development of this field.

Published
2021

16. A Pareto-based collaborative multi-objective optimization algorithm for energy-efficient scheduling of distributed permutation flow-shop with limited buffers

Author

Leilei Meng, Yuanxiang Huang, Biao Zhang, Liang Gao, Chao Lu, and Jiajun Zhou

Subjects
Mathematical optimization, education.field_of_study, Job shop scheduling, Computer science, General Mathematics, Population, Pareto principle, Initialization, Flow shop scheduling, Industrial and Manufacturing Engineering, Computer Science Applications, Scheduling (computing), Permutation, Control and Systems Engineering, Metric (mathematics), education, Software
Abstract

Energy-efficient scheduling of distributed production systems has become a common practice among large companies with the advancement of economic globalization and green manufacturing. Nevertheless, energy-efficient scheduling of distributed permutation flow-shop problem with limited buffers (DPFSP-LB) does not receive adequate attention in the relevant literature. This paper is therefore the first attempt to study this DPFSP-LB with objectives of minimizing makespan and total energy consumption ( T E C ). To solve this energy-efficient DPFSP-LB, a Pareto-based collaborative multi-objective optimization algorithm (CMOA) is proposed. In the proposed CMOA, first, the speed scaling strategy based on problem property is designed to reduce T E C . Second, a collaborative initialization strategy is presented to generate a high-quality initial population. Third, three properties of DPFSP-LB are utilized to develop a collaborative search operator and a knowledge-based local search operator. Finally, we verify the effectiveness of each improvement component of CMOA and compare it against other well-known multi-objective optimization algorithms on instances. Experiment results demonstrate the effectiveness of CMOA in solving this energy-efficient DPFSP-LB. Especially, the CMOA is able to obtain excellent results on all problems regarding the comprehensive metric, and is also competitive to its rivals regarding the convergence metric.

Published
2022

17. Biphasic extraction of different polysaccharides from Radix Sophorae Tonkinensis by microwave-assisted aqueous two-phase extraction: Process optimization, structural characterization and mechanism exploration

Author

Yuyang Lin, Xuehao Wu, Ke Wang, Shiyu Zhou, Yuanxiang Huang, Xiujuan Xie, Chujun Fu, Laicheng Luo, Wei Zhang, and Huajun Fan

Subjects
chemistry.chemical_classification, Chromatography, Aqueous solution, 010405 organic chemistry, Extraction (chemistry), Filtration and Separation, 02 engineering and technology, 021001 nanoscience & nanotechnology, 01 natural sciences, High-performance liquid chromatography, 0104 chemical sciences, Analytical Chemistry, Gel permeation chromatography, chemistry.chemical_compound, Hydrolysis, chemistry, Monosaccharide, Response surface methodology, 0210 nano-technology, Derivatization
Abstract

A novel method for biphasic extraction and separation of different polysaccharides from Radix Sophorae Tonkinensis (RST) was developed by microwave-assisted aqueous two-phase extraction (MAATPE) in a one-step procedure. Using ethanol/Na2HPO4 system as a green extractant, the effects of the composition of the aqueous two-phase system (ATPS), extraction temperature and extraction time were investigated by single-factor experiments coupled to response surface methodology (RSM) through UV–vis analysis. Under the optimum conditions, extraction yields of two polysaccharides from top and bottom phases were 8.52 ± 0.21% (w/w) and 2.18 ± 0.08% (w/w), respectively. Compared with heating solvent extraction (HSE) and ultrasonic assisted extraction (UAE), MAATPE exhibited higher extraction efficiency. By means of Fourier transform infrared spectroscopy (FT-IR) and high-performance gel permeation chromatography (HPGPC), two polysaccharides obtained from both phases had the characteristics of homogenous heteropolysaccharides, but there were some differences in chemical structures and the molecular weight from each other. Both polysaccharides could be extracted respectively from RST to the top ethanol-rich phase and the bottom salt-rich phase due to their polarity difference. Furthermore, the chemical compositions of two polysaccharides were confirmed by high-performance liquid chromatography (HPLC) analysis after hydrolysis and derivatization, the molar ratios of monosaccharides were glucose: arabinose: galactose: rhamnose: mannose: glucuronic acid: aminogalactose = 63.70: 18.21: 7.00: 5.10: 3.13: 1.78: 1.07 for the polysaccharide in the top phase and 75.24: 3.79: 3.71: 3.37: 2.51: 1.43: 9.95 for those in the bottom phase, respectively. By means of extraction process analysis and scanning electron microscopy (SEM), the exportation of MAATPE mechanism revealed that combining microwave-assisted extraction (MAE) with aqueous two-phase extraction (ATPE) not only improved the extraction yields, but also obtained at least two different polysaccharides. Under microwave field, the ATPS could make the herb cell ruptured, and multiphase mass-transfer was accelerated. The proposed method is a rapid, simple and efficient alternative to biphasic extraction of diverse polysaccharides from natural plants.

Published
2018

18. Microwave-assisted aqueous two-phase extraction of alkaloids from Radix Sophorae Tonkinensis with an ethanol/Na2HPO4 system: Process optimization, composition identification and quantification analysis

Author

Laicheng Luo, Yuyang Lin, Shiyu Zhou, Huajun Fan, Yuanxiang Huang, Xuehao Wu, James Z. Tang, Xiujuan Xie, and Wei Zhang

Subjects
Aqueous solution, Ethanol, Chromatography, 010405 organic chemistry, Chemistry, 010401 analytical chemistry, Extraction (chemistry), 01 natural sciences, 0104 chemical sciences, Solvent, chemistry.chemical_compound, Yield (chemistry), Process optimization, Radix, Response surface methodology, Agronomy and Crop Science
Abstract

A rapid method for simultaneous extraction and separation of multiple alkaloids from Radix Sophorae Tonkinensis (RST) was developed by microwave-assisted aqueous two-phase extraction (MAATPE) using the aqueous two-phase extraction system (ATPS) of ethanol/Na2HPO4 as the extraction solvent. The effects of key factors on extraction yield were investigated by utilizing single-factor experiment coupled to response surface methodology (RSM). The regression model by RSM was significant (p

Published
2018

19. Atorvastatin alleviates microglia-mediated neuroinflammation via modulating the microbial composition and the intestinal barrier function in ischemic stroke mice

Author

Junmin Chen, Rong Chen, Cong Zhang, Lan Zhang, Guang Shi, Peipei Zhang, Xiangjian Zhang, Wenyan Shang, and Yuanxiang Huang

Subjects
0301 basic medicine, Atorvastatin, Gut flora, Pharmacology, Occludin, Biochemistry, Proinflammatory cytokine, Brain Ischemia, 03 medical and health sciences, Mice, 0302 clinical medicine, Physiology (medical), Medicine, Animals, cardiovascular diseases, Neuroinflammation, Barrier function, Ischemic Stroke, biology, Microglia, business.industry, biology.organism_classification, Transplantation, Mice, Inbred C57BL, Stroke, 030104 developmental biology, medicine.anatomical_structure, business, 030217 neurology & neurosurgery, medicine.drug
Abstract

Our previous work has shown that atorvastatin exerts anti-inflammatory properties in ischemic stroke, and recent studies have revealed that intestinal microbiota plays a vital role in the pathogenesis of stroke. However, it is not clear whether the anti-inflammatory effects of atorvastatin against ischemic stroke is related to gut function and microbiota. We report herein that atorvastatin significantly ameliorated the defects in sensorimotor behaviors and reduced microglia-mediated neuroinflammation by inhibiting proinflammatory polarization of microglia in the peri-infarct cortex of the mice with permanent middle cerebral artery occlusion (pMCAO). Moreover, atorvastatin reversed microbial composition (characterized by increased abundance of Firmicutes and Lactobacillus and decreased Bacteroidetes abundance), increased fecal butyrate level, promoted intestinal barrier function (elevated protein levels of claudin-1, occludin and mucoprotein 2), as well as regulated intestinal immune function (decreased MCP-1, TNF-α and increased IL-10). Atorvastatin also significantly reduced the level of circulating endotoxin (lipopolysaccharide-binding protein), which is a biomarker of leaky gut. Transplantation of fecal microbiota collected from atorvastatin treated mice potently attenuated neuroinflammation in pMCAO mice, and the anti-inflammatory effects of fecal microbiota transplantation were similar to those of oral atorvastatin administration. These results suggested that the atorvastatin-mediated restoration of gut microbiota, improvement of intestinal barrier function and regulation of intestinal immunity were involved in the anti-inflammatory function in stroke mice.

Published
2020

20. Pentoxifylline enhances antioxidative capability and promotes mitochondrial biogenesis for improving age-related behavioral deficits

Author

Tianyun Zhang, Hui Zhao, Geming Shi, Rui Cui, Yuanxiang Huang, Yu Wang, Guoliang Zhang, Wensheng Yan, Yunxiao Kang, Chunxiao Qi, and Xiaoming Ji

Subjects
Male, medicine.medical_specialty, Aging, mitochondrial biogenesis, Phosphodiesterase Inhibitors, Substantia nigra, Antioxidants, Pentoxifylline, Rats, Sprague-Dawley, chemistry.chemical_compound, Internal medicine, medicine, Animals, Cyclic adenosine monophosphate, NRF1, Phosphodiesterase inhibitor, Maze Learning, Organelle Biogenesis, Behavior, Animal, Chemistry, Dopaminergic, Brain, Cell Biology, TFAM, aged rats, Mitochondria, Rats, Endocrinology, pentoxifylline, Mitochondrial biogenesis, antioxidative capability, medicine.drug, Research Paper
Abstract

Pentoxifylline (PTX) is a non-specific phosphodiesterase inhibitor with pleiotropic effects that is routinely used to treat peripheral vascular disease. In this study, we tested whether PTX could also counteract the detrimental effects of aging in the brain. To accomplish that, we treated aged rats with PTX and measured resulting behavioral alterations as well as changes in dopaminergic neurochemical levels, oxidative balance markers, mitochondrial function, nuclear factor erythroid 2-related factor 2 (Nrf2), peroxisome proliferator activated receptor-gamma coactivator 1-alpha (PGC-1α) and downstream gene expression, and cyclic adenosine monophosphate (cAMP) content in the brain. The results demonstrated that PTX improved motor and cognitive deficits and restored levels of dopamine and its metabolites in the brains of aged rats. PTX also reduced malondialdehyde levels and increased the GSH/GSSG ratio, mitochondrial ATP, nuclear Nrf2, and cAMP levels, and upregulated PGC-1α, nuclear respiratory factor 1, and mitochondrial transcription factor A expression in the substantia nigra and hippocampus of aged rats. Thus, increased nuclear Nrf2 levels and upregulation of PGC-1α, which enhance antioxidative capability and promote mitochondrial biogenesis, may be responsible for PTX-induced amelioration of behavioral deficits in aged rats.

Published
2020

21. sj-pdf-2-imr-10.1177_0300060520943452 - Supplemental material for Efficacy and safety of human urinary kallidinogenase for acute ischemic stroke: a meta-analysis

Author

Yuanxiang Huang, Binglei Wang, Zhang, Yue, Peize Wang, and Xiangjian Zhang

Subjects
111199 Nutrition and Dietetics not elsewhere classified, Cardiology, 170199 Psychology not elsewhere classified, 111799 Public Health and Health Services not elsewhere classified, 110604 Sports Medicine, FOS: Health sciences, 110306 Endocrinology, 110308 Geriatrics and Gerontology, 111099 Nursing not elsewhere classified, 111708 Health and Community Services, 160807 Sociological Methodology and Research Methods, 111702 Aged Health Care, 111403 Paediatrics, 110904 Neurology and Neuromuscular Diseases, 110203 Respiratory Diseases, 110315 Otorhinolaryngology, FOS: Clinical medicine, 110319 Psychiatry (incl. Psychotherapy), FOS: Sociology, FOS: Psychology, 110599 Dentistry not elsewhere classified, 110323 Surgery, 110305 Emergency Medicine, 111599 Pharmacology and Pharmaceutical Sciences not elsewhere classified, 111299 Oncology and Carcinogenesis not elsewhere classified, 110314 Orthopaedics
Abstract

Supplemental material, sj-pdf-2-imr-10.1177_0300060520943452 for Efficacy and safety of human urinary kallidinogenase for acute ischemic stroke: a meta-analysis by Yuanxiang Huang, Binglei Wang, Yue Zhang, Peize Wang and Xiangjian Zhang in Journal of International Medical Research

Published
2020
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22. sj-pdf-1-imr-10.1177_0300060520943452 - Supplemental material for Efficacy and safety of human urinary kallidinogenase for acute ischemic stroke: a meta-analysis

Author

Yuanxiang Huang, Binglei Wang, Zhang, Yue, Peize Wang, and Xiangjian Zhang

Subjects
111199 Nutrition and Dietetics not elsewhere classified, Cardiology, 170199 Psychology not elsewhere classified, 111799 Public Health and Health Services not elsewhere classified, 110604 Sports Medicine, FOS: Health sciences, 110306 Endocrinology, 110308 Geriatrics and Gerontology, 111099 Nursing not elsewhere classified, 111708 Health and Community Services, 160807 Sociological Methodology and Research Methods, 111702 Aged Health Care, 111403 Paediatrics, 110904 Neurology and Neuromuscular Diseases, 110203 Respiratory Diseases, 110315 Otorhinolaryngology, FOS: Clinical medicine, 110319 Psychiatry (incl. Psychotherapy), FOS: Sociology, FOS: Psychology, 110599 Dentistry not elsewhere classified, 110323 Surgery, 110305 Emergency Medicine, 111599 Pharmacology and Pharmaceutical Sciences not elsewhere classified, 111299 Oncology and Carcinogenesis not elsewhere classified, 110314 Orthopaedics
Abstract

Supplemental material, sj-pdf-1-imr-10.1177_0300060520943452 for Efficacy and safety of human urinary kallidinogenase for acute ischemic stroke: a meta-analysis by Yuanxiang Huang, Binglei Wang, Yue Zhang, Peize Wang and Xiangjian Zhang in Journal of International Medical Research

Published
2020
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23. Combination of microwave-assisted extraction and ultrasonic-assisted dispersive liquid-liquid microextraction for separation and enrichment of pyrethroids residues in Litchi fruit prior to HPLC determination

Author

Huajun Fan, Ke Wang, Yuanxiang Huang, Shiyu Zhou, Yi Zhang, Yuyang Lin, Wei Zhang, and Xiujuan Xie

Subjects
Detection limit, Chromatography, Trace Amounts, Liquid Phase Microextraction, Chemistry, 010401 analytical chemistry, Extraction (chemistry), General Medicine, 010402 general chemistry, 01 natural sciences, High-performance liquid chromatography, 0104 chemical sciences, Analytical Chemistry, Solvent, chemistry.chemical_compound, Litchi, Chlorobenzene, Fruit, Pyrethrins, Ultrasonics, Response surface methodology, Microwaves, Enrichment factor, Chromatography, High Pressure Liquid, Food Science
Abstract

A novel method for simultaneous determination of pyrethroids residues in Litchi fruit has been developed by HPLC-UV detection using microwave-assisted extraction (MAE) coupled with ultrasonic-assisted dispersive liquid-liquid microextraction (UADLLME). Extraction conditions of MAE and UADLLME were respectively investigated by single-factor experiments and response surface methodology. Optimized experimental conditions included 310μL of chlorobenzene as extraction solvent, 1.3mL of ethanol as dispersive solvent and 3min of extraction time for UADLLME. In the case of MAE, extraction temperature of 70°C, extraction time of 4min and solvent-to-materials ratio of 40:1 were adopted. Results demonstrated that the proposed method had good performance with linearity of 0.0050-4.98mg/L, recovery of 83.3-91.5%, RSDs below 5.6% and detection limit (LOD) of 1.15-2.46μg/L for six pyrethroids, offering higher extraction efficiency and larger enrichment factor. MAE-UADLLME provided a sensitive and efficient alternative to determination of trace amounts of pesticides residues in food samples.

Published
2018

24. Preparation and application of aptamer-functionalized sorbent for the analysis of ultra-trace aflatoxin M1 and analogues in milk

Author

Huajun Fan, Yi Zhang, Yuanxiang Huang, Wu Shaojing, and Zhenfeng Yue

Subjects
Detection limit, Aflatoxin, Sorbent, Chromatography, Elution, Chemistry, Aptamer, 010401 analytical chemistry, Extraction (chemistry), 02 engineering and technology, 021001 nanoscience & nanotechnology, 01 natural sciences, 0104 chemical sciences, Analytical Chemistry, Adsorption, 0210 nano-technology, Colorimetric analysis, Spectroscopy
Abstract

A novel, efficient and specific aptamer-functionalized sorbent was successfully synthesized and applied in the analysis of ultra-trace aflatoxin M1 (AFM1) and its analogues in milk. By using a gold nanoparticle-based colorimetric analysis tool, we could determine that the AFM1-aptamer (M1-aptamer) recognized and bound AFM1 to generate an aptamer-target complex. After analysis and verification, the M1-aptamer on the surface of the carboxyl microspheres were completely modified via EDC/NHs amide formation. The effects of extraction pH, extraction buffer, concentration of Mg2+ and elution solvents were investigated by single-factor experiments to determine the most suitable analysis conditions. Using these conditions, the extraction performance of the M1-aptamer-functionalized sorbent could be studied and an analytical method for AFM1 and its analogues in milk could be established. The results of adsorption equilibrium as well as adsorption selectivity experiments indicated that the M1-aptamer offered a specific adsorption site to selectively adsorb aflatoxins exhibiting a dihydrofuran oxaphthalene scaffold. The adsorption capacity of the aptamer-functionalized sorbent was 233.1 μg/g. A studied method for the determination AFM1 in milk with low matrix effect demonstrated a good linear correlation in the range of 0 to 2.0 μg/L (R2 = 0.9982) and high detection sensitivity (detection limits of 7 ng/kg in milk and 21 ng/kg in milk power). Furthermore, excellent spiked recovery and good repeatability were obtained. The method delivered specific, accurate and reproducible results for enriching and detecting ultra-trace AFM1 and its analogues in milk.

Published
2021

25. LncRNAs down-regulate Myh1, Casr, and Mis18a expression in the Substantia Nigra of aged male rats

Author

Yannan Ma, Qiqing Guo, Shufeng Liu, Rui Cui, Xu Feng, Yuanxiang Huang, Xiaoming Ji, Geming Shi, Guoliang Zhang, and Yunxiao Kang

Subjects
Male, Aging, Future studies, Transcription, Genetic, Mrna expression, Down-Regulation, Substantia nigra, Biology, long non-coding RNAs, Male rats, Animals, aged male rats, KEGG, Messenger RNA, Myosin Heavy Chains, Gene Expression Profiling, RNA, Cell Biology, RNA, Circular, Cell biology, Rats, Blot, Substantia Nigra, RNA, Long Noncoding, messenger RNAs, Receptors, Calcium-Sensing, Research Paper, circular RNAs
Abstract

In this study, we used high-throughput RNA sequencing to identify mRNAs, long non-coding RNAs (lncRNAs) and circular RNAs (circRNAs) that are differentially expressed in the Substantia Nigra (SN) of aged and young rats. Gene Ontology and Kyoto Encyclopedia of Genes and Genomes pathway analyses were used to perform functional annotation of mRNAs that were either differentially expressed themselves (DEMs), targeted by differentially expressed lncRNAs (DELs), or the parents of differentially expressed circRNAs (DECs). A total of 112 DEMs, 163 DELs, and 98 DECs were found in the SN of aged rats. The down-regulated lncRNA NONRATT010417.2 targeted the down-regulated mRNA Myh1, while the down-regulated lncRNA NONRATT015586.2 and the up-regulated lncRNAs NONRATT000490.2 and NONRATT007029.2 all targeted the down-regulated mRNAs Casr and Mis18a. Western blots and RT-qPCR revealed that Myh1, Casr, and Mis18a protein and mRNA expression were significantly reduced in aged rats compared to young rats. This study improves our understanding of the transcriptional alterations underlying aging-related changes in the SN and provides a foundation for future studies of associated molecular mechanisms.

Published
2019

26. Microwave-assisted aqueous two-phase extraction of diverse polysaccharides from Lentinus edodes: Process optimization, structure characterization and antioxidant activity

Author

Wei Zhang, Hang Lin, Ke Wang, Chao Chen, Huiyun Zeng, Huajun Fan, Shiyu Zhou, Yuyang Lin, Yuanxiang Huang, and Pingfan Li

Subjects
Ammonium sulfate, DPPH, Chemical structure, Shiitake Mushrooms, 02 engineering and technology, Chemical Fractionation, Polysaccharide, Biochemistry, 03 medical and health sciences, chemistry.chemical_compound, Structural Biology, Microwaves, Molecular Biology, 030304 developmental biology, chemistry.chemical_classification, 0303 health sciences, Aqueous solution, Chromatography, biology, Extraction (chemistry), Monosaccharides, Temperature, Water, Fungal Polysaccharides, General Medicine, Free Radical Scavengers, 021001 nanoscience & nanotechnology, biology.organism_classification, Solvent, Molecular Weight, chemistry, Lentinus, Solvents, 0210 nano-technology
Abstract

Microwave-assisted aqueous two-phase extraction (MAATPE) was applied to simultaneous extraction of diverse polysaccharides from Lentinus edodes with aqueous two-phase extraction system (ATPS) of ethanol/ammonium sulfate as the biphasic extractant. Key factors in MAATPE process were optimized by response surface methodology, and the optimum conditions were concluded as follows: the ATPS composition of ethanol concentration 26.0% (w/w) and (NH4)2SO4 concentration 19.58% (w/w), extraction temperature 78.7 °C, extraction time 19.55 min and solid-to-solvent ratio 1:50. The extraction yields of the polysaccharides from the top phase (PTP) and the bottom phase (PBP) were 2.12 ± 0.21% and 11.16 ± 0.28%, respectively. By means of FT-IR, HPGPC and HPLC analysis, PTP and PBP were different from each other in chemical structure, their average molecular weight (MW) were respectively 119,533 Da and 3906 Da. Also, the mechanism of MAATPE process was explored by composition analysis and scanning electron microscopy. Both polysaccharides exhibited stronger antioxidant activities, EC50 values of scavenging DPPH, hydroxyl and superoxide radicals for PBP achieved 580 ± 3, 83.1 ± 3.6 and 91.1 ± 0.5 μg/mL as against 560 ± 4, 76.3 ± 2.2 and 89.3 ± 2.4 μg/mL of PTP, respectively. Compared with conventional methods using a mono-phase solvent, MAATPE can not only greatly improve extraction efficiency, but also selectively extract diverse polysaccharides.

Published
2019

27. Efficacy and safety of human urinary kallidinogenase for acute ischemic stroke: a meta-analysis

Author

Binglei Wang, Peize Wang, Xiangjian Zhang, Yuanxiang Huang, and Yue Zhang

Subjects
0301 basic medicine, acute ischemic stroke, China, Medicine (General), medicine.medical_specialty, Urinary system, Urology, Urine, Kallikrein kinin system, Biochemistry, Brain Ischemia, 03 medical and health sciences, R5-920, 0302 clinical medicine, Humans, Medicine, kallikrein–kinin system, Adverse effect, Stroke, Acute ischemic stroke, Ischemic Stroke, business.industry, Meta Analysis, Human urinary kallidinogenase, Biochemistry (medical), stroke treatment, Cell Biology, General Medicine, medicine.disease, United States, meta-analysis, Stroke treatment, 030104 developmental biology, Meta-analysis, adverse effects, business, Tissue Kallikreins, 030217 neurology & neurosurgery
Abstract

Objective Human urinary kallidinogenase (HUK) is a glycoprotein extracted from human urine that is used to treat stroke by triggering positive regulation of the kallikrein–kinin system. Our aim was to evaluate the efficacy and safety of HUK treatment for acute ischemic stroke. Methods We searched the online databases PubMed, Embase, Cochrane Library, Google Scholar, and China National Knowledge Infrastructure (CNKI) for papers published between January 2015 and December 2019. The quality of each trial was assessed using the Cochrane Reviewers’ Handbook. Randomized controlled trials of HUK in patients with acute ischemic stroke were included. Results Sixteen trials with 1326 participants were included. The HUK injection groups had more neurological improvement than the control groups in National Institutes of Health Stroke Scale scores (mean difference, –1.65; 95% confidence interval [CI], –2.12 to –1.71) and clinical efficacy (1.30; 95% CI, 1.21 to 1.41). Subgroup analysis indicated that age may influence heterogeneity. Eleven trials reported adverse effects and there were no significant differences between the control and HUK groups (risk difference, 0.01; 95% CI, –0.02 to 0.04). Conclusions HUK ameliorates neurological symptoms in stroke patients with few adverse effects. Further high-quality, large-scale randomized trials are needed to confirm these results.

Published
2020

28. Haloperidol ameliorates androgen-induced behavioral deficits in developing male rats.

Author

Chunxiao Qi, Xiaoming Ji, Guoliang Zhang, Yunxiao Kang, Yuanxiang Huang, Rui Cui, Shuangcheng Li, Huixian Cui, and Geming Shi

Subjects
HALOPERIDOL, NEUROBEHAVIORAL disorders, TESTOSTERONE, PROPIONATES, DOPAMINERGIC mechanisms
Abstract

The purpose of present study was to infer the potential effects of testosterone increase in some male-based childhood-onset neuropsychiatric disorders, such as Tourette syndrome. Thus, the influence of early postnatal androgen exposure upon the neurobehaviors and its possible neural basis were investigated in the study. Male pup rats received consecutive 14-day testosterone propionate (TP) subcutaneous injection from postnatal day (PND) 7. The TP treatment produced the hyperactive motor behavior and grooming behavior as well as the increased levels of dopamine, tyrosine hydroxylase and dopamine transporter in the mesodopaminergic system and the elevated levels of serotonin in the nucleus accumbens, without affecting the levels of glutamate, γ-aminobutyric acid, norepinephrine and histamine in the caudate putamen and nucleus accumbens of PND21 and PND49 rats. Dopamine D2 receptor antagonist haloperidol was administered to the early postnatal TP-exposed PND21 and PND49 male rats 30 min prior to open field test. Haloperidol significantly ameliorated the motor behavioral and grooming behavioral defects induced by early postnatal TP exposure. The results demonstrated that early postnatal androgen exposure significantly disturbed the brain activity of developing male rats via enhancing the mesodopaminergic activity. It was suggested that abnormal increments of testosterone levels during the early postnatal development might be a potential risk factor for the incidence of some male-based childhood-onset neuropsychiatric disorders by affecting the mesodopaminergic system. [ABSTRACT FROM AUTHOR]

Published
2018
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