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To evaluate the effect of platelet rich fibrin (PRF) combining with Bio-oss in treating Class II furcation involvement.Thirty patients who had Class Ⅱ furcation involvement in the mandibular first molar were included. They were all free of systemic disease. After initial periodontal therapy, they were randomly divided into 2 groups. In the experimental group, PRF combining with Bio-oss were placed in the areas with furcation defect and covered with PRF. In the control group, only flap surgery was performed. All patients were followed up for 6 months after operation. The efficacy was evaluated with clinical parameters and cone-beam computed tomography (CBCT). The data were analyzed with SPSS 2.0 software package.Periodontal indexes including probing depth (PD), clinical attachment loss (CAL), horizontal probing depth (HPD) significantly decreased in both groups after operation (P0.05); alveolar bone significantly increased in the experimental group (P0.05).The clinical effect of PRF combined with Bio-oss on Class II furcation involvement are remarkable.

On the basis of analyzing the development status of the wireless communication technique, a new method to analyze the wireless communication reliability is proposed by using the Received Signal Strength Indication (RSSI). A wireless network model based on module CC2530PA is built, which is composed with a coordinator and a few terminal nodes. By analyzing the data received coordinator and sent terminal nodes, the relations of SER, PER and "pseudo-loss" phenomenon whit RSSI are studied, the model of relation between PER and RSSI is built, analyzing the reliability of wireless communication and threshold value of received data via the model. The research results have very high practical value and will promote the development of wireless communication in the future.

The aim of the present study was to evaluate the clinical value of 125I particles implantation in the treatment of recurrent bladder cancer. The study is a retrospective analysis of 32 patients with recurrent bladder cancer treated between May 2010 and January 2010. Of these, 16 cases (chemotherapy group) received conventional chemotherapy. A total of 16 patients (125I group) received radiotherapy with 125I particles, followed by conventional chemotherapy. By guidance of B ultrasound, 125I radioactive particles were implanted. All 32 patients were relieved after treatment, and the tumors were significantly reduced after 2 months. However, the tumors in the 125I group were significantly smaller than those in the chemotherapy group (P

The aim of the present study was to evaluate the clinical value of 125I particles implantation in the treatment of recurrent bladder cancer. The study is a retrospective analysis of 32 patients with recurrent bladder cancer treated between May 2010 and January 2010. Of these, 16 cases (chemotherapy group) received conventional chemotherapy. A total of 16 patients (125I group) received radiotherapy with 125I particles, followed by conventional chemotherapy. By guidance of B ultrasound, 125I radioactive particles were implanted. All 32 patients were relieved after treatment, and the tumors were significantly reduced after 2 months. However, the tumors in the 125I group were significantly smaller than those in the chemotherapy group (P

This paper proposes a vision orientation system based on OpenCV to solve the orientation problem that glass curtain wall cleaning robots meet with at work. The system applies edge detection and the Hough transform to the images captured by the monocular camera, then judges whether the robot has reached glass edges according to the results of histogram comparison between the target image and the template image. Finally the system sends corresponding orders to the robot for path selection and orientation.

In order to remove the hidden danger resulted by distortion of weak surrounding rock in tunnel, the GM(1,1) of the distortion data of weak surrounding rock is used to predict the distortion trend of weak rock vault in the next phase. According to the actual data, a GM(1,1) of the distortion is built, and it is shown that the predict result of GM(1, 1) is accurate, and the calamity can be reduced by the way.

Some patients who have heterotrophic ossification (HO) in a postburn scar are occasionally seen in the clinic. The occurrence of HO following hip and abdominal surgery is common in males, whereas females are frequently affected following burns. The HO of skin grafts in burn scars of the superior body parts is uncommon, especially on the face. We report a recent case of a male patient in whom HO arose on skin grafts on his chin. The patient presented with a history of a painless chin mass for 48 years. Computed tomography scan suggested that the mass was located within subcutaneous tissue away from the lower jaw. The density of the mass was almost the same as the cortical bone of the lower jaw. The mass was completely resected under general anesthesia. It was a rectangular mass of 3× 0.5× 0.3 cm. Histological examination gave the diagnosis of HO, which was also confirmed by immunohistochemical stainings for vimentin. Recurrence was not found in this case by clinical and radiologic follow-up at 1 year after surgery.

CRD method and three-bench method with temporary invert, which are applied to shallow buried and large cross-section tunnel engineering, are discussed, and simulated by FLAC2D so as to study the variation feature of displacement field, surrounding rock disturbance and internal supporting force caused by the excavation. According to the simulation results, it is proved that CRD method, with higher safety, can control tunnel collapse effectively while three-bench method quickens construction schedule. Therefore, it is recommended that the two methods are used for excavating tunnels alternately if possible so that it will increase economic benefit.

We investigated whether baseline vessel status evaluated by magnetic resonance angiography (MRA) can be the foremost factor to classify acute ischemic stroke patients into subgroups for thrombolytic therapy within 3-6 hours of symptom onset.Acute ischemic stroke patients beyond 3 hours after symptom onset were examined by stroke magnetic resonance imaging (MRI) (diffusion- and perfusion-weighted imaging, and MRA) before and after thrombolysis treatment within 24-48 hours. Stroke MRI was used to classify acute ischemic stroke patients into subgroups and select optimal patients for thrombolytic treatment. Clinical scores were compared to determine whether there were significant differences among subgroups.The difference in day 90 modified Rankin scale (mRS) between treated salvageable and untreated salvageable patients with recombinant tissue plasminogen activator (rt-PA) was remarkably statistically significant (p=0.02). Treated salvageable patients had more favorable clinical outcomes as compared with the untreated salvageable patients. Patients who did not have baseline artery occlusion were associated with more favorable clinical outcomes than untreated salvageable patients (p0.001). The difference between treated salvageable and patients without artery occlusion in 90 day mRS score was not statistically significant (p=0.058).Baseline vessel status evaluated by MRA may be used as the first factor ahead of mismatch to categorize acute ischemic stroke patients into subgroups. Patients who do not have initial vessel occlusion may not need thrombolytic therapy.

This study was aimed to investigate the antiproliferative effect of escopoletin on CAL 27 and CAL 33 oral squamous cancer cell lines. MTT assay and flow cytometry were used for the analysis of escopoletin effect on cell viability and apoptosis, respectively. Western blot analysis was used for the examination of cyclin and cyclin-dependent kinase expression after treatment of CAL 33 cells with escopoletin. The results showed a marked decrease in the viability of CAL 27 and CAL 33 cell lines after 48 h of escopoletin treatment. Treatment of CAL 33 cells with escopoletin led to the induction of apoptosis and arrest of cell cycle at G0/G1 phase. In the cells treated with escopoletin cyclin D1 and E expression was reduced and CDK1 expression was inactivated. The above findings suggest that escopoletin exhibits inhibitory effect on the oral squamous cancer through induction of apoptosis and arrest of cell cycle. Therefore escopoletin can be a promising candidate for the prevention of oral squamous cancer.

To investigate the differentiation and osteogenic activity of Naringin-induced bone marrow stromal cells (BMSCs) in dogs.BMSCs were separated and cultured in vitro and identified by FCM. Then different concentration of Naringin (1×10⁻⁵, 1×10⁻⁶, 1×10⁻⁷, 1×10⁻⁸ and 1×10⁻⁹ mol/L) were added to cell culture media to induce BMSCs. The effect of Naringin on BMSCs was evaluated respectively by CCK-8 method and measuring the activity of alkaline phosphatase (ALP). The formation of nodules of calcium was detected by von Kossa staining. The data was analyzed with SPSS20.0 software package.The result of cell-surface marker displayed that the expression of CD34 and CD45 were negative while the expression of CD90 was positive. The values were 0.126%, 0.075% and 95.4%, respectively. Naringin could obviously promote cell proliferation, which exhibited the best effect on proliferation and osteogenic differentiation at concentration of 10-6 mol/L. Calcium nodule (von Kossa) staining was positive.Naringin-induced bone marrow stromal cells can be differentiated into osteoblasts. Naringin at the concentration of 10-6 mol/L can enhance the proliferation and osteogenic differentiation of BMSCs.

To evaluate the clinical effect of modified casting post with zirconia crown in restoration of anterior teeth.A total of 45 anterior teeth (32 patients) were treated with modified gold-alloy post-cores with zirconia crowns. All roots were greatly tilted to the labial side. Marginal integrity, anatomic form, surface, color and the gingival situation after restorations were assessed at 6 and 12 months with the California Dental Association (CDA) quality assessment system.The clinical outcome of 44 zirconia crowns achieved rank A (97.8%), while the gingival situation in one patient (2.2%) reached rank B.Modified gold-alloy post-core with zirconia crown contributes to the aesthetic effect of the anterior teeth which need to change the angle.

To establish the model of alveolar ridge preservation after tooth extraction for dental implant replacement, and to observe the effect of tissue engineered bone on osseointegration.Isolated BMSCs were expanded and osteogenically induced in vitro. The tissue engineering complex was constructed with BMSCs/A-PCPC in vitro. Six extraction sockets, with three on each side, were created in the mandibles of four Beagle dogs by extracting the second, third and fourth premolars. BMSCs/A-PCPC were placed on one side of the extraction sockets, while autogenous bone, A-PCPC and nothing were placed on the other side as control. X-ray and CT scans were conducted 1day, 4 and 12 weeks after operation to detect the change of the alveolar ridge. The bone of sockets were harvested at 8-week post-implantation and subject to histological for evaluating. SPSS17.0 software package was used for data analysis.Radiographs demonstrated higher radiodensity in group of complex than in simple materials group, autogenous bone group after 4 weeks. Hard tissue biopsy at 12-week showed that bone activity of BMSCs/A-PCPC complex was better than the other groups. Spiral CT analysis showed that alveolar ridge of each group experienced a certain degree of absorption. At 12-week, the alveolar ridge height reduction values in A-PCPC group was smaller than in A-PCPC group, autogenous bone group and blank group (P0.05).The BMSCs/A-PCPC complex is favorable for preservation of alveolar ridge.

To evaluate the measurement accuracy by multi-slice spiral CT(MSCT) and dental software as a tool for preoperative design before tooth implantations.Twenty volunteers with marker template underwent MSCT. The diameter of the front teeth crowns in the bucco-lingual direction were measured by MSCT using a standard dental software package. Postoperatively, the same distances were clinically measured using a sliding caliper. The consistency between the two measurements was analyzed by paired t test using SPSS17.0 software package.The difference of measurements was -0.04-0.21mm. There was no significant difference between the CT and the manual measurement of the crown diameter in the bucco-lingual direction(P0.05).The results demonstrate that MSCT and marker template promises to be a valuable method for the measurement of jaw implant regions.

There exist bigger error of fault model and few examining data in traction power supply system. A novel way to model fault for few data is advanced, its parameter is estimated by maximum likelihood criterion and the model is optimized via least square law. The model solves effectively the problem that modeling few data in traction power supply system relies big swatch theory, and the model of fault data of the contact net in railway is built. By fitting result, it is proved that the precision of fitting data is bigger improved.

Because there exists the ambiguity in complex systems, and there is the limitation in traditional fault tree, a novel fault tree analysis theory is introduced. In the theory, the probability grey number, which can express the event's subjective ambiguity and objective ambiguity, is introduced to express the degree and probability that the components go wrong, dynamic envelope is applied to score the relation among components, and a new logic gate, Grey-gate, is advanced for expressing the effect of system reliability when the components go wrong. Finally, the theory of fault diagnosis is applied to analyze the fault effect of the system with software and hardware.

To study the osteogenic capability of the construct combined dog's bone stromal cells with active porous calcium phosphate cement (A-PCPC) in nude mice in vivo.Isolated bone marrow stromal cells (BMSCs) were expanded and osteogenically induced in vitro. Their osteogenic phenotype was evaluated by cytochemistry. The tissue engineering complex was constructed with BMSCs/A-PCPC in vitro. After SEM scanning, the complex of BMSCs/A-PCPC was implanted into the subcutaneous tissue of the nude mice as experimental group, and A-PCPC as control group. The engineered bone was harvested 2,4,8weeks post-implantation and processed for HE staining, then evaluated by histology and histomorphometry.Cytochemistry showed alkaline phosphate activity, Von Kossa staining proved the formation of mineralization nodules. Scanning electron microscopy showed the cells adhered to the inner surface of the A-PCPC. HE staining showed a small group of woven bone formation 2 weeks later in the experimental group, while the formation of bone less in the control group. Woven bone turned into trabecular bone gradually at 4 weeks in the experimental group, while the control group showed a large number of bone-like tissue. Histomorphometry showed more mature bone in the experimental group than the control group at 8 weeks.The A-PCPC/BMSCs composites show good osteogenetic activity and could promote mineralization of the immature bone. It can be used as the bone tissue engineering scaffolds. Supported by Innovation Fund for Science and Technology Development of Pudong New District(Grant No. PKJ2009-Y19).

To construct siRNA-VHL expression vector and detect the effect of VHL gene interference on BMSCs.According to the dog's VHL gene sequences, four pairs of siRNA oligo were designed and synthesized. Using vector cloning kit reorganization, four pairs of double-stranded siRNA were inserted into the expression vector (pcDNA™ 6.2-GW/EmGFPmiR) and 4 siRNA expression plasmids (SR144-1,SR144-2,SR144-3,SR144-4) were constructed. With the vector universal primers, colony PCR was screened. The positive clones were sequenced to verify whether the sequence of insert fragments in recombinant clones was consistent with oligo sequences designed or not. Interference vector transiently transfected the BMSCs. qPCR and Western blot were used to detect the gene silencing effect. In order to improve transfection efficiency of siRNA-VHL as well as the effect of the VHL gene silencing, pLenti-mi-VHL was constructed.Through sequencing the plasmids cloned, the fragment sequences inserted in recombinant clones were consistent with the designed oligo sequences. After 24 h and 48 h transfection of BMSCs cells by plasmids, SR144-4 showed the best effect of interference by qPCR and Western blot. Through comparing the sequencing results, the inserted fragment sequences were completely correct and the pLenti-mi-VHL was successfully constructed.The siRNA-VHL expression vector for BMSCs is successfully constructed and applicable for further experiments. Supported by Research Fund of Science and Technology Commission of Shanghai Municipality (Grant No.9411954800) and Foundation for Open Project from Shanghai Key Laboratory of Stomatology (Grant No.S30206).

To observe the biocompatibility of new biomaterials porous calcium phosphate (CPC) and ectopic bone formation of CPC with bone marrow stromal cells (BMSCs).The BMSCs were cultured from Beagle dog and combined with the porous CPC with the best concentration after transfect green fluorescent protein (GFP). The adhesion and growth of BMSCs on CPC were observed under inversion, fluorescence and scanning electron microscopy. The ectopic bone formation were observed at the 8th week after CPC and BMSCs were implanted subcutaneously into nude mice.When BMSCs with CPC were cultured at the 1st day, cells were climbing out from CPC with normal morphology. At the 7th day cells can be seen protruding pseudopods, secretion of matrix. Bone formation could be seen histomorphologically at the 8th week.Porous CPC has good biocompatibility and is an ideal scaffold material for bone tissue engineering.

To investigate the stress distribution of 2 kinds of distal cantilevered fixed prostheses supported by 4 or 6 dental implants in edentulous mandible with three-dimensional finite element method.The first design was 4 implants supported fixed prostheses with 6mm distal cantilever; the second design was 6 implants supported fixed prostheses with 6mm distal cantilever. Vertical force of 100 N was applied to the distal cantilever of fixed prostheses.The bone stress was concentrated at the cortex of mandible. The maximum bone stress was at the cortex near the second implants from the point that the vertical force was applied. The maximum implant stress was at the terminal and medium implant-prostheses interface.The bone around the terminal implant is easily to absorb and the terminal and medium implants are easily to fracture. The maximum stress of the implants and prostheses of two kinds of design are almost same(239Mpa). Two kinds of design can be applied in the clinic. 6 mm distal cantilevered fixed prostheses supported by 6 implants have the less bone stress than 4 mm distal cantilevered fixed prostheses in edentulous mandible.

Qualitative simulation, now, has been a powerful tool to solve incomplete knowledge problems. During qualitative simulation, Chattering variable is one of the important causes that QSIM creates intractable branch, and identifying chattering variables and eliminating the chattering with available method is a key technology of improving operation rate and predicting accuracy of QSIM. We studied carefully the "Higher-Order Derivative Constraints" theory of Kuipers, and found that there are some problems in the theory. So we advanced a series of improved methods to identify chattering variables and to eliminate chatter of variables. Meanwhile we tried to use effectual system knowledge and qualitative information implicating in QDE to eliminate the chatter of variables.

Objective To investigate the effects of miR-25-3p on the occurrence, development and proliferation of tongue squamous cell carcinoma cells. Methods To establish tongue squamous cell carcinoma cell line Tca8113 that stably and highly express miR-25-3p using recombinant retroviral vector-mediated gene transfer method. The proliferation of transfected Tca8113 was detected by thiazolyl blue tetrazolium bromide (MTT) and cell colony formation assays. cyclinD1, p21 cip1 and p27 kip1 mRNA expressions in the transfected Tca-8113 were detected by quantitative PCR. cyclinD1, p21, p27 kip1 , AKT, p-AKT, FOXO1 and p-FOXO1 expressions in the transfected Tca8113 were detected by western blot analysis. In addition, miR-25-3p expression in the tongue squamous cell carcinoma cell line and tissue specimen was also detected by quantitative PCR. Results Quantitative PCR showed that miR-25-3p expression in the tongue squamous cell carcinoma cell lines and tissue specimen was significantly lower than that in the adjacent tissue. MTT and cell colony formation assays showed that after miR-25-3p overexpression, the proliferation of transfected Tca8113 was obviously attenuated. Western blot analysis and quantitative PCR showed that after miR-25-3p overexpression, p21 cip1 and p27 kip1 expressions were upregulated, while cyclinD1, AKT, FOXO1 expressions were downregulated, and AKT and FOXO1 phosphorylation was inactivated in the transfected Tca8113 cells. Conclusions MiR-25-3p inhibited the proliferation of tongue squamous cell carcinoma cells and regulated cell cycle-related protein expression, playing an important role in the occurrence and development of squamous cell carcinoma of the tongue.