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Hinged multi-body systems are gaining popularity in the field of ocean engineering. Their performance is commonly evaluated using numerical simulations, but comparisons with experimental data are required to ensure the accuracy of the computational tools. However, there is a dearth of experimental studies on the motion performance of hinged multi-body systems, particularly those involving more than two hinged floating bodies. This study aims to fill this gap in experimental data for hinged multi-body systems beyond two bodies. The rectangular box was chosen as the test model due to its stable hydrodynamic properties and ease of numerical modelling. Five identical boxes were prefabricated and subsequently tested in the pool in a sequence ranging from one to five boxes to capture the motion performance. Additionally, a numerical programme based on potential flow theory was developed for mutual validation with the experimental data. Firstly, the physical properties of each box were determined through equations calculation and a free decay test, enabling the acquisition of all parameters for conducting numerical simulations. Then, the response amplitude operator (RAO) curves for the heave and pitch motion of a single box were depicted, and the results indicated that the resonant frequency in pitch direction obtained from the regular wave test was consistent with that obtained from the free decay test. Finally, the motion RAO curves of hinged multi-body systems were presented and analysed. The agreement between the measured and computed results confirms the suitability of the experimental data presented in this study as benchmark data for validating numerical simulations. [ABSTRACT FROM AUTHOR]

To minimize energy expenditure for each individual, animals adopt distinctive formations, such as fish schooling, "V" formation by flying birds, and single-file formation by waterfowls. The phenomenon of ducklings following their mothers in a single-file configuration has been revealed by the mechanisms of wave-riding and wave-passing. Drawing inspiration from this phenomenon, an investigation is undertaken on ships moving in a single-file formation. The objective is to quantify how much energy can be saved in different configurations. In this study, a three-dimensional boundary element method incorporating linear free-surface boundary conditions is used to obtain the wave drag and wave patterns. It is found that when constructive wave interference occurs in a two-ship formation, the wave resistance of the trailing ship increases and the leading ship experiences a decrease in its wave drags, especially when the two ships are in close proximity. Mutual benefit arises when destructive wave interference occurs between two ships. In addition, increasing the size of the trailing vessel facilitates the effect of wave-riding by the leading ship, but this effect becomes less pronounced as the speed increases. In a multi-ship formation configuration, changing the size of the leading ship will have a localized effect on the wave-passing, but the fleet will eventually tend to a dynamic equilibrium. When the position of the first trailing vessel is changed, there is similarly a localized effect on the wave-passing. Adjusting the first trailing ship to the position of the constructive wave interference is not favorable to reducing its own drag but enhances the wave-riding effect of its close follower. Finally, to achieve wave-passing, the trailing ship does not necessarily have to occupy an optimum position. This can still be accomplished if the trailing ship moves backward by an integer multiple of wavelength. [ABSTRACT FROM AUTHOR]

Ship hydrodynamics in a constant waterway have been well studied. With a coordinate system fixed on the moving ship, the boundary value problem (BVP) is usually treated as a steady one. However, the hydrodynamics of a ship moving in confined waterways with abrupt changes in width or depth are complex due to their unsteady nature. The studies on such unsteady problems are insufficient. In the engineering practice, the hydrodynamic unsteadiness can be fully reflected by a scenario when a ship enters a lock. Prior studies have predominantly focused on predicting the ships' hydrodynamic forces without considering the unsteady terms on the free-surface boundary conditions. Obviously, such steady or quasi-steady methods overlooked the crucial unsteady phenomena on free water surface. To address this gap, the present study introduces a novel three-level difference scheme to discretize the free surface condition, preserving unsteady terms while maintaining temporal continuity of cells on the free surface. With the implementation of such fully unsteady BVP, we observed some interesting unsteady free surface motions, which were not well documented in the existing literature. To verify our new observations, as well as to validate the numerical method proposed in this study, two physical model tests were designed and conducted in a towing tank: a submerged ellipsoid enters a deep lock at relatively high speeds, and a box enters a shallow and narrow lock at very low speeds. The discussion is highlighted on the unsteady waves in front of the moving bodies, as well as the unsteady resistance induced by such waves. [ABSTRACT FROM AUTHOR]

West Nile virus (WNV), an arthropod-borne flavivirus, can cause severe symptoms, including encephalitis, and death, posing a threat to public health and the economy. However, there is still no approved treatment or vaccine available for humans. Here, we developed a novel vaccine platform based on a classical insect-specific flavivirus (cISF) YN15-283-02, which was derived from Culicoides. The cISF-WNV chimera was constructed by replacing prME structural genes of the infectious YN15-283-02 cDNA clone with those of WNV and successfully rescued in Aedes albopictus cells. cISF-WNV was nonreplicable in vertebrate cells and nonpathogenic in type I interferon receptor (IFNAR)-deficient mice. A single-dose immunization of cISF-WNV elicited considerable Th1-biased antibody responses in C57BL/6 mice, which was sufficient to offer complete protection against lethal WNV challenge with no symptoms. Our studies demonstrated the potential of the insect-specific cISF-WNV as a prophylactic vaccine candidate to prevent infection with WNV. [ABSTRACT FROM AUTHOR]