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Additional file 16. Review history.

Additional file 2: Fig S2. α-Diversity analysis (Shannon index) among the GX.DG, GX.SG, HB, and NC groups. Experimental description references to Fig. 2.

Additional file 9: Fig S8. Copy numbers of B. pseudolongum and B. animalis in feces. (A) Survival rate of mice infected with a LD50 dose of GX virus (n=20) or equivoluminal PBS (NC group, n=10). (B and C) On day 2 and 10 post-infection, fecal samples were collected, and the DNA was extracted respectively. After 15 days infection, the pre-extracted DNA of fecal samples were divided into two groups based on whether the corresponding mice died or survived from the infection. Then, qRT-PCR was performed to detect the copy numbers of B. pseudolongum(B) and B. animalis(C). The data are presented as the mean ± SD. Statistics for the copy number was two-way ANOVA. *P

Additional file 8: Fig S7. LDA effect size (LEfSe) comparison of gut microbes at species level in the GX.SG and GX.DG groups. Experimental description references to Fig. 3. (A–E) LEfSe analysis of gut microbes collected on days 5 (A), 8 (B), 9 (C), 10 (D), and 11 (E) post-infection.

Additional file 5: Fig S5. Classification of bacterial genera based on their variation trends in the four mouse groups. Experimental description references to Fig. 2. (A) Bacteria whose abundance increased only in the GX.DG group. (B) Bacteria whose abundance first increased and then decreased only in the GX.DG group. (C) Bacteria whose abundance increased in both the GX.DG and GX.SG groups. (D) Bacteria whose abundance decreased only in the GX.DG group. (E) Bacteria whose abundance increased in the GX.DG, GX.SG, and HB groups, with the greatest increase in the HB group.

Additional file 4: Fig S4. Bacterial phyla showing significant differences in abundance, other than those shown in Fig. 2c. Experimental description references to Fig. 2. The data are presented as the relative abundance of specific microbiota at the phylum level in indicated groups and were analyzed by Wilcoxon-Mann-Whitney test, at *P

Additional file 6 Fig S6. Rarefaction curves. The rarefaction analysis involved random sampling with replacement and estimation of the total number of genes that were identified in the samples. The values represent that the curve in all group is near smooth when the sample number are great enough with few new genes detected.

Additional file 3: Fig S3. Principal coordinate analysis (PCoA) of the weighted UniFrac distances among the GX.DG, GX.SG, HB, and NC groups at four infection stages. Experimental description references to Fig. 2. Sample clustering at stage 4 (days 9-15) revealed differences between the GX.DG group and the other three groups.