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Purpose: to investigate the level anti-pertussis IgM, IgG, IgA in pregnant women in the I — II — III trimesters. Materials and methods. A total of 288 pregnant women aged. 17 — 43 years (in the I — II — III trimesters) were examined. A serums samples tested for IgM, IgG, IgA against pertussis by ELISA of RIDASCREEN (Germany). Results. Anti-pertussis IgG concentration below the low limit of detection occurred in 75,7% of pregnant women. The majority of seropositive among pregnant women was in the age group 26 — 35 years (82,9%). The proportion of seronegative among pregnant women increased. with increasing pregnancy periods, as well as depending on age. In III trimester IgG were detected in 18,2% of pregnant women. The most seronegative among pregnant women were in the III trimester and at the age of over 36 years old. The detection of IgM, IgG and IgA made it possible to detect the active infection process in 11 (3,9%) pregnant women by elevated level of IgA. High IgA level only in combination with IgM was in serum sample from 1 pregnant woman, high IgA level in combination with IgG at negative values of IgM was in serum samples from 8 pregnant woman. Only IgA in serum sample from 2 pregnant women (I and III trimesters of pregnancy) were detected. This is probably due to the presence of whooping cough or mucosal contamination with B.pertussis (persisting IgA). Conclusion. The serological studies have shown the need to develop algorithms for protecting newborns — from, the moment pregnant women are registered, to the onset of childbirth. These algorithms will provide information about on the presence of whooping cough and will help prevent infection of the newborn.

The aim of the work was to characterize the radiation incidents that took place in the territory of the Voronezh region. Materials and methods. To prepare this article the stock data of the radiological laboratory of the Center for Hygiene and Epidemiology in the Voronezh Region for 2009-2018 was used. All measurements in the investigation of radiation incidents were carried out with the MKS-AT1117M dosimeter-radiometer which passed the state calibration. The equivalent dose rate of gamma radiation (μSv/h) was determined. Results. For the last 10 years, 8 radiation incidents were registered on the territory of the Voronezh region, which were related to domestic clocks - 4 (measurements according to citizens), the detection of orphan sources of ionizing findings - 2, scrap metal - 1 and a thermometer - 1 (during customs inspection). According to the classification of emergencies, the radiation incidents are local, i.e. do not go beyond the territory of the facility, according to the international scale of nuclear and radiological events, INES refer to the incident (anomalies) with the level 1 “minor problems with the safety of components, a radioactive source of low activity level”. All interactions in the investigation of radioactive incidents involving specialists from the radiological laboratory of the Center for Hygiene and Epidemiology in the Voronezh region with other services showed a good level of rapid response.

The purpose of the study was to estimate the doses to the Voronezh region population from natural sources of ionizing radiation and the technologically altered background in the territory of Voronezh Region. The data of forms of state statistical observation No. 4-DOZ “Information on the doses of population exposure due to natural and technologically altered background” for 20102017 and the radiation and hygienic passport of the territory of the Voronezh Region were used. The average annual per caput effective dose due to all types of ionizing radiation remains stable with a slight upward trend and lies in the range from 2.925 (2010) to 3.656 mSv (2017). Natural sources are the main dose-forming factors for the population. Their annual contribution to the annual effective dose ranges from 74.96 to 83.65%. The leading contribution to the total dose from natural sources is the exposure due to the inhalation of radon isotopes: it ranges from 37.6 to 51.1%. In second place,there is the share of external exposure from sources of terrigenous origin, which ranges from 21.2 to 28.9% of the total dose. The average annual effective dose of natural exposure to humans varies from 2,355 to 2,980 mSv / year, the exposure from radon – from 0,83 to 1,65 mSv / year. The dose from technogenic-altered radiation background, including global radioactive fallout due to atmospheric nuclear tests and due to past radiation accidents are insignificant (0,062 mSv / year). Its annual contribution to the total dose is less than 2%. Based on the results of the assessment of the indicators characterizing the level of exposure of sources of ionizing radiation to natural and technogenic-altered radiation background, no excess of radiation safety standards has been recorded. The situation associated with exposure to ionizing radiation sources in the Voronezh region has been described as safe for the last 8 years.

The purpose of the study was to assess the doses of personnel and the population at the expense of all the main activities and sources of radiation in the territory of the Voronezh region. The data of the forms of state statistical supervision No. 1-DOZ “Information on the doses of personnel from persons under normal use of technogenic sources of ionizing radiation”, No. 3-DOZ “Information on radiation doses of patients during X-ray radiology studies”), No. 4-DOZ “Information on radiation doses of the population due to natural and technogenically altered background” for 2010-2016 and the radiation and hygienic passport of the territory of the Voronezh Region. Based on the results of monitoring the radiation situation, the situation associated with the impact of ionizing radiation sources in the Voronezh Region has been characterized as safe for the past 7 years. The average annual effective dose per 1 inhabitant due to all ionizing radiation remains stable with a slight upward trend and lies in the range from 2.925 (2010) to 3.399 mSv (2016). In the structure of the collective dose of the population of the Voronezh region, the dose from natural sources is 83.65%, from medical sources – 16.06%, from technogenically changed background radiation, including global fallout and accident at the Chernobyl nuclear power plant – 0.18%, from the activities of enterprises using Sources of ionizing radiation – 0.11%. The average annual effective dose of natural exposure to humans varies from 0.660 to 0.704 mSv / year, natural radiation from radon from 0.832 to 1.465 mSv / year. The average effective dose from medical research for the procedure for the study period was 0.27-0.40 mSv and tends to decrease due to the introduction of modern low-dose medical diagnostic equipment. On the territory of the Voronezh region, there were no population groups with an effective radiation dose exceeding 5 mSv / year. Gamma-background in the region in 2010-2016. Did not exceed the natural level and amounted to 0.08 – 0.16 μSv / h. The excess of hygienic standards for the effects of ionizing radiation sources on humans has not been recorded.

The aim of the study was personnel dose assessment due to the normal operations with the artificial radiation sources. The article is based on the data from the Unified System of Individual Dose Control and Voronezh Region’s radiation-hygienic passport. The data from No.1-DOZ “Information on personnel exposure doses under normal operation of technogenic ionizing radiation sources” and over a period of 2006-2010 years were analyzed. In 2006-2015, the number of organizations, which submitted form No.1-DOZ “Information on personnel exposure doses under normal operation of technogenic ionizing radiation sources”, increased from 175 to 229. In amount of the radiation facilities, Novovoronezh Nuclear Power Plant is the first. Novovoronezh NPP has 1512 sources, which amounts to 51,9% from all sources in Voronezh Region (2915). Health care facilities have 869 radiation sources or 29,8%. X-ray machines are the main part of these sources (844 health care facilities or 97,1% of all medical sources). Industrial sources occupy third place with 305 facilities or 10,5% of all considered sources. In 2015, according to the data from Voronezh Region’s radiation-hygienic passport, the number of “A” group personnel were 4237, the number of “B” group personnel were 2341. The average individual dose for personnel was over the range from 0.66 to 2.02 mSv. Collective dose was from 4.16 to 11.79 man-sieverts per year. The increase of number of the radiation sources has attended with the decrease of individual and collective doses. The most likely it is related to using the modern facilities. In 2015, the maximum value of the average individual dose of “A” group personnel was registered in Voronezh regional hospital (6.17 mSv y–1). There are medical facilities with unsealed and sealed sources in this hospital. In 2006-2015, the average individual doses of personnel of all radiation facilities that use radiation sources in Voronezh region were significantly below the dose limits established by the Radiation Safety Standards NRB-99/2009.

The study aimed at radiation contamination impact assessment due to the 1986 Chernobyl accident in Voronezh Region territory more than 600 kilometers away from the ground zero. The major Chernobyl accident impact assessment indicators were the characteristics of 137Cs and 90Sr radionuclides’ soil surface contamination (Ci/km2) as well as the average annual effective dose of critical population group ( mSv/year) over 1986–2014. The Population oncological morbidity indicators were analyzed (all malignant neoplasms, including those in thyroid gland, lymphatic and hematopoietic tissue) in the territories contrastingly differing on the levels of radiation factor impact. The study covered the period of 2010–2014.It was established that for Voronezh Region territories referred to as the post- Chernobyl radioactively contaminated zone over 29 years period the maximum soil surface contamination by 137Cs and 90Sr radionuclides reduced by 1.90 and 1.91 times (from 3,15 Ci/km2 to 1,66 Ci/km2 and from 0,063 Ci/km2 to 0,0033 Ci/km2, respectively).Currently the relationship was not found between the radioactive contamination density in Voronezh Region and the levels of malignant neoplasms for the local residents.The present situation related to radiation factor impact on Voronezh Region territories remains stable and safe. Mindful of the indicators results the assessment of ionizing sources impact did not identify any exceeding the normative values.

Different species of small mammals (SM) (539 total), cached in Voronezh region, were examined for antigen and DNA of Francisella tularensis. The basic species of SM involved in circulation of F. tularensis were revealed. The features of the biotopic distribution of infected SM on the territory of the natural foci were shown. The diversity of species SM ensures long operation and epizootic activity of the natural foci of tularemia.

Aim. Improvement of monitoring and prognosis of epidemic manifestations of natural foci of tularemia on the territory of Voronezh region using immune-serological and molecular-genetic study of main carriers of the disease. Materials and methods. 539 small mammals captured during summer period of 2011 in 4 districts ofNorth-Eastern part ofVoronezh region were studied. Animal organs were studied by serologic (search for Francisella tularensis antigens) and molecular-biologic (detection of F. tularensis DNA) methods. Tularemia antigen was detected using passive hemagglutination reaction (PHAR) with erythrocytic tularemia immunoglobulin diagnosticum. Real-time polymerase chain reaction (RT-PCR) was applied for detection of tularemia causative agent DNA. Results. Complex study revealed epizootic activity of natural foci of tularemia in the examined territory. F. tularensis antigen and/or DNA were detected in 82 objects (15.2%). Use of RT-PCR allowed to additionally detect samples with relatively low content of F. tularensis DNA substrate, when antigen was not detected in samples. High sensitivity and specificity of the RT-PCR was ensured by inclusion of specific probes (tul4-PR2 and ISFTu2P). Conclusion. The results obtained give evidence on functioning and epizootic activity of natural foci of tularemia in Voronezh region that requires constant monitoring of the territory and prophylaxis measures, first of all vaccination of risk groups by live tularemia vaccine.