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2. Development of Magnetic Particle-Based Immunoassays and Their Applications in Diabetes Monitoring

Author

Chien-An Chen, Yu-Yin Tsai, Yi-Yen Yuan, Ting-Shou Chen, Jane S. C. Tsai, Cheng-Tai Chen, and Yi-Chau Huang

Subjects
chemistry.chemical_classification, Chromatography, biology, Polymer, Magnetic particle inspection, Horseradish peroxidase, Electronic, Optical and Magnetic Materials, chemistry.chemical_compound, Glycated albumin, chemistry, Diabetes monitoring, biology.protein, Magnetic nanoparticles, Glycated hemoglobin, Polystyrene, Electrical and Electronic Engineering
Abstract

In addition to blood glucose testing, monitoring of blood glycated proteins got considerable attention on the glycemic control of diabetes. Glycated albumin has been reported as a good complementary to or better indicator than glycated hemoglobin for the evaluation of short-term glycemic control. Herein, the development of magnetic particle-based immunoassays for blood glycated proteins is demonstrated. Spherical magnetic particles are fabricated composed of a polystyrene core covered with a thin layer of Fe3O4 and SiO2. The core-shell structure allow us to control a wide size range by using the polystyrene cores of different size, and the silica outer layer can be functionalized easily with silane coupling agents. Aminophenylboronic acid is covalently immobilized onto magnetic particles for the affinity isolation of glycated proteins, while horseradish peroxidase labeled antibodies are used for detection. The assays are optimized and applied successfully to the determination of glycated albumin in standard and human serum samples and show good potential as tools for diabetes monitoring.

Published
2014

3. Microfluidic device by using nano-magnetic beads conjugated antibodies for immunoassay

Author

Chung-Hsien Tsai, Chih-Hsiu Yin, Hsiao-Chung Tsai, Yuh-Jiuan Lin, Yu-Yin Tsai, and Hui-Ju Cho

Subjects
Cartridge, Materials science, Chromatography, medicine.diagnostic_test, Health professionals, Immunoassay, Microfluidics, Nano, medicine, Nanotechnology, Conjugated system, Biomedical equipment
Abstract

We developed a fast and precision immunoassay-based microfluidic assay system that consisted of a microfluidic analytic cartridge and immunoassay reagents for point-of-care testing (POCT). The nano-magnetic beads conjugated antibodies, HRP-labeled analog, and color reagent were all pre-loaded and packaged in a microfluidic analytic cartridge. The whole assay process was completed within 30 min without any additional washing or loading of reagents once the sample was injected in the microfluidic analytic cartridge. The performance of the immunoassay-based microfluidic assay system had been examined by using mouse IgG model. The results indicated that the dynamic range of the measurement could be approached to two orders with low deviation. The present study demonstrated that the performance of our immunoassay-based microfluidic assay system was suitable for POCT for healthcare professionals or patients.

Published
2009

4. Peritoneal dialysis - 1

Author

Fatma Eroglu, Claudio Pozzi, Hyunwook Kim, Claudio Ronco, Benjamin Zeier, Xueqing Yu, Dirk G. Struijk, Javier Ocaña, Roman Stankiewicz, Marilena Cara, Taner Camsari, Jun Wada, Susan Yung, Hung Shih-Yuan, Fu-Chang Hu, Michał Nowicki, Ni Gao, Denise E. Sampimon, Deirisa Lopes Barreto, Zulfikar Yilmaz, Martin Zeier, Johan V. Povlsen, Daniele Ciurlino, Zofia Wankowicz, Yu-Yin Tsai, Rungmei S. K. Marak, Renzo Scanziani, Chang Ming-Yu, Iraj Najafi, Xiao Yang, Kazumichi Matsushita, Show-Mei Nian, Tak Mao Chan, Chunyan Yi, Yung-Ming Chen, Manel Vera, Eduard Garcia, Cheng-Hsu Chen, Laura Buzzi, Jens Dam Jensen, Petar Kes, Hung-Chun Chen, Almudena Ortigosa, Zeljka Mustapic, Wing-Fai Pang, Paula López-Sánchez, Christian Thiede, Carlo Crepaldi, Vedat Schwenger, Marie-Luise Gross, Giovambattista Virga, Qingyu Kong, Aiping Gu, Jenq-Wen Huang, Manuel Corral, Gao Dan, Ching-Yuang Lin, Liang Xian-hui, Wim Van Biesen, Isao Araki, Tetsu Miyamoto, Ivana Jurić, Roberto Russo, Xiuqing Dong, Francisco Coronel, Erjola Likaj, Ramazan Cetinkaya, Wieslawa Lysiak-Szydlowska, Chi-Chih Hung, Uta Oelschlaegel, Cheuk-Chun Szeto, Shigeru Akagi, Yuji Takatori, Ulrike Haug, Chih-Kang Chiang, Julia Kerschbaum, Veysi Akpolat, Per Ivarsen, Qiang Yao, Elena Alberghini, Lee Yi-Jer, Claudio Musetti, Bengt Lindholm, Mei-Chuan Kuo, Paweł Stróżecki, Michael A. Rudnicki, Teresa Grzelak, Aiwu Lin, Catherine Chen, Lars P. Kihm, Young Chul Yoon, Masahito Tamura, Qing Zhang, Mihaela Petti, A. Lawerence, Dong Hyung Lee, Shih-Tin Huang, Jun Wu, Xinghui Lin, Beom Seok Kim, Luciana Bonfante, Elżbieta Marcinkowska, Mariapia Rodighiero, Marijke de Graaff, Silvia Furiani, June Fabian, Tatsuya Miyamoto, Elena Corchete, Myftar Barbullushi, Manabu Kamiyama, Anna Stefańska, Sandra Müller-Krebs, Shin Wook Kang, Hasan Kayabasi, Mercedes Velo, Enzo Corghi, Kai-Ming Chow, Zbigniew Heleniak, Nikolina Bašić-Jukić, Raymond T. Krediet, Constanze Meye, Min-Ju Wu, Zhao Zhanzheng, Ryota Serino, Pierluigi di Loreto, Nicole Ladeira, Monika Lichodziejewska-Niemierko, Nader Nouri-Majalan, Hoshang Sanadgol, Gülgün Oktay, Raj Kumar Sharma, Yoshiaki Doi, Beata Szary, Anupma Kaul, Katarzyna Osiewala, Ivano Baragetti, Shang-Jyh Hwang, Paul König, Kuo-Hsiung Shu, Kuan-Yu Hung, M. Emin Yilmaz, Francisco Maduell, Haeng Soon Jeong, Yumi Furuno, Maurizio Nordio, Andrea Wagner, Hameed Anijeet, Jacek Rysz, Hye Ran Kim, Xiaoyan Li, Philip Kam-Tao Li, Ilaria Serra, Zhaohui Ni, Ya-Wen Chuang, Ali Kemal Kadiroglu, Marta Arias, Vincenzo La Milia, Yucheng Yan, José Portolés, Jacek Manitius, Liu Zhangsuo, Sagren Naidoo, Snjezana Glavas-Boras, Aliyu Abdu, Bolesław Rutkowski, Chi-Hung Cheng, Juan M. López-Gómez, Chia-Te Liao, Kay Herbrig, Joanna Stachowska-Pietka, Mieko MIyazaki, Xin Wang, Peter Gross, Tun-Jun Tsai, Weiying Chen, Jacek Waniewski, Mario R. Korte, Efsun Kolatan, Dorota Bielińska − Ogrodnik, Keiichi Takiue, Tatsuya Shibata, Eun Young Kim, Isabel Devolder, Ho Li-Chun, Zahide Cavdar, Makoto Hiramatsu, Si Hyun Kim, Mayte Ribera, Marcia R. Gómez, Rafał Donderski, Sylwia Małgorzewicz, Shirin Malgas, Dede Sit, Dariusz Nowak, Hamidullah Uyanik, Tobias Drescher, Ming-Ju Wu, Annick Verleysen, Robert A. Stolarek, Junko Inoue, Aggrey Mweemba, Aleix Cases, Grzegorz Zelichowski, Krystyna Czyżewska, Ho Yung Lee, Nagahiro Sato, Yutaka Otsuji, Jeong Hwan Shin, Shoichiro Kojo, Jiaqi Qian, Yang Wook Kim, Jens Passauer, Johann Hausdorfer, Osman Yilmaz, Hirofumi Makino, Tae Ik Chang, Ahmet Duraku, Tzu-Hui Chen, Jianxiong Lin, Hitoshi Sugiyama, Magdalena Grajewska, J.M. Campsitol, Yeong Hoon Kim, Jung Tak Park, Raymond Vanholder, Chi-Bon Leung, Chen-Yen Kuo, Anniek Vlijm, Li Yan, Hsin-Hui Wang, Takashi Yamagishi, Jae Hyun Chang, Jin-Bor Chen, Antonio Alcaraz, Patricia de Sequera, Jolanta Fijalkowska, Wang Hsi-Hao, Anna Olszowska, Rammohan Bhat, Mizuya Fukasawa, Graham Paget, Jocelyn T Naicker, Saimir Seferi, Silvio Bertoli, Wei Fang, Gert Mayer, Ling Ye, Mustafa Keles, Su-Chu Lee, Masashi Suzuki, Kwan-Dun Wu, Saraladevi Naicker, Denise Sampimon, Shang-Chih Liao, Kyu Hun Choi, Bonnie Ching-Ha Kwan, Sarasadat Moghadasimousavi, Giorgio Vescovo, Adrianna Pedzik, Muhammad Imran, Weiming Zhang, Narutoshi Kabashima, Funda Saglam, Tae Hyun Yoo, Martin Bornhäuser, Tung-Min Yu, Ryoko Baba, Nestor Thereska, Sun Young Park, Masami Bessho, Sulen Sarioglu, Hamit Acemoglu, Hiroshi Morinaga, Kazushi Nakao, Abdullah Uyanik, Andrzej Werynski, Masayuki Takedda, Dong Ki Kim, Jeong Nyeo Lee, Bang-Gee Hsu, Bergadá E, Shouji Kudo, and Néstor Fontseré

Subjects
Transplantation, medicine.medical_specialty, Nephrology, business.industry, medicine.medical_treatment, medicine, Urology, business, Peritoneal dialysis
Published
2009

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