31 results on '"Yu Han Gao"'
Search Results
2. Targeting central nervous system extracellular vesicles enhanced triiodothyronine remyelination effect on experimental autoimmune encephalomyelitis
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Yun Xiao, Jing Tian, Wen-Cheng Wu, Yu-Han Gao, Yu-Xin Guo, Sheng-Jiao Song, Rui Gao, Li-Bin Wang, Xiao-Yu Wu, Yuan Zhang, and Xing Li
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Extracellular vesicles ,Neuroinflammation ,Multiple sclerosis ,Drug delivery ,Experimental autoimmune encephalomyelitis ,T3 ,Materials of engineering and construction. Mechanics of materials ,TA401-492 ,Biology (General) ,QH301-705.5 - Abstract
The lack of targeted and high-efficiency drug delivery to the central nervous system (CNS) nidus is the main problem in the treatment of demyelinating disease. Extracellular vesicles (EVs) possess great promise as a drug delivery vector given their advanced features. However, clinical applications are limited because of their inadequate targeting ability and the “dilution effects” after systemic administration. Neural stem cells (NSCs) supply a plentiful source of EVs on account of their extraordinary capacity for self-renewal. Here, we have developed a novel therapeutic system using EVs from modified NSCs with high expressed ligand PDGF-A (EVPs) and achieve local delivery. It has been demonstrated that EVPs greatly enhance the target capability on oligodendrocyte lineage. Moreover, EVPs are used for embedding triiodothyronine (T3), a thyroid hormone that is critical for oligodendrocyte development but has serious side effects when systemically administered. Our results demonstrated that systemic injection of EVPs + T3, versus EVPs or T3 administration individually, markedly alleviated disease development, enhanced oligodendrocyte survival, inhibited myelin damage, and promoted myelin regeneration in the lesions of experimental autoimmune encephalomyelitis mice. Taken together, our findings showed that engineered EVPs possess a remarkable CNS lesion targeting potential that offers a potent therapeutic strategy for CNS demyelinating diseases as well as neuroinflammation.
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- 2022
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3. Trends in burden of multidrug-resistant tuberculosis in countries, regions, and worldwide from 1990 to 2017: results from the Global Burden of Disease study
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Ze-Jin Ou, Dan-Feng Yu, Yuan-Hao Liang, Wen-Qiao He, Yong-Zhi Li, Ya-Xian Meng, Hu-Sheng Xiong, Min-Yi Zhang, Huan He, Yu-Han Gao, Fei Wu, and Qing Chen
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Multidrug-resistant tuberculosis ,Global burden of disease ,Age-standardized rate ,Estimated annual percentage change ,Epidemiological trend ,Infectious and parasitic diseases ,RC109-216 ,Public aspects of medicine ,RA1-1270 - Abstract
Abstract Background Antituberculosis-drug resistance is an important public health issue, and its epidemiological patterns has dramatically changed in recent decades. This study aimed to estimate the trends of multidrug-resistant tuberculosis (MDR-TB), which can be used to inform health strategies. Methods Data were collected from the Global Burden of Disease study 2017. The estimated annual percentage changes (EAPCs) were calculated to assess the trends of MDR-TB burden at global, regional, and national level from 1990 to 2017 using the linear regression model. Results Globally, the age-standardized rate (ASR) of MDR-TB burden including incidence, prevalence, death and disability-adjusted life years (DALYs) had pronounced increasing trends from 1990 to 1999, with the EAPCs were 17.63 [95% confidence interval (CI): 10.77–24.92], 17.57 (95% CI 11.51–23.95), 21.21 (95% CI 15.96–26.69), and 21.90 (95% CI 16.55–27.50), respectively. Particularly, the largest increasing trends were seen in areas and countries with low and low-middle sociodemographic index (SDI). However, the trends in incidence, prevalence, death and DALYs of MDR-TB decreased globally from 2000 to 2017, with the respective EAPCs were − 1.37 (95% CI − 1.62 to − 1.12), − 1.32 (95% CI − 1.38 to − 1.26), − 3.30 (95% CI − 3.56 to − 3.04) and − 3.32 (95% CI − 3.59 to − 3.06). Decreasing trends of MDR-TB were observed in most regions and countries, particularly that of death and DALYs in Slovenia were − 18.96 (95% CI − 20.82 to − 17.06) and -19.35 (95% CI − 21.10 to − 17.55), respectively. Whereas the pronounced increasing trends of MDR-TB occurred in Papua New Guinea, Singapore, and Australia. Conclusions The ASR of MDR-TB showed pronounced decreasing trends from 2000 to 2017. However, the MDR-TB burden remains a substantial challenge to the TB control globally, and requires effective control strategies and healthcare systems.
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- 2021
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4. Composition of gut and oropharynx bacterial communities in Rattus norvegicus and Suncus murinus in China
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Wen-qiao He, Yi-quan Xiong, Jing Ge, Yan-xia Chen, Xue-jiao Chen, Xue-shan Zhong, Ze-jin Ou, Yu-han Gao, Ming-ji Cheng, Yun Mo, Yu-qi Wen, Min Qiu, Shu-ting Huo, Shao-wei Chen, Xue-yan Zheng, Huan He, Yong-zhi Li, Fang-fei You, Min-yi Zhang, and Qing Chen
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Rattus norvegicus ,Suncus murinus ,Bacterial composition ,Next-generation sequencing ,Veterinary medicine ,SF600-1100 - Abstract
Abstract Background Rattus norvegicus and Suncus murinus are important reservoirs of zoonotic bacterial diseases. An understanding of the composition of gut and oropharynx bacteria in these animals is important for monitoring and preventing such diseases. We therefore examined gut and oropharynx bacterial composition in these animals in China. Results Proteobacteria, Firmicutes and Bacteroidetes were the most abundant phyla in faecal and throat swab samples of both animals. However, the composition of the bacterial community differed significantly between sample types and animal species. Firmicutes exhibited the highest relative abundance in throat swab samples of R. norvegicus, followed by Proteobacteria and Bacteroidetes. In throat swab specimens of S. murinus, Proteobacteria was the predominant phylum, followed by Firmicutes and Bacteroidetes. Firmicutes showed the highest relative abundance in faecal specimens of R. norvegicus, followed by Bacteroidetes and Proteobacteria. Firmicutes and Proteobacteria had almost equal abundance in faecal specimens of S. murinus, with Bacteroidetes accounting for only 3.07%. The family Streptococcaceae was most common in throat swab samples of R. norvegicus, while Prevotellaceae was most common in its faecal samples. Pseudomonadaceae was the predominant family in throat swab samples of S. murinus, while Enterobacteriaceae was most common in faecal samples. We annotated 33.28% sequences from faecal samples of S. murinus as potential human pathogenic bacteria, approximately 3.06-fold those in R. norvegicus. Potential pathogenic bacteria annotated in throat swab samples of S. murinus were 1.35-fold those in R. norvegicus. Conclusions Bacterial composition of throat swabs and faecal samples from R. norvegicus differed from those of S. murinus. Both species carried various pathogenic bacteria, therefore both should be closely monitored in the future, especially for S. murinus.
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- 2020
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5. Study of circulating Th17 cells and associated cytokines in myasthenia gravis
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Yu-han GAO, Hai-qiang JIN, Hong-jun HAO, Yong-an SUN, and Feng GAO
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myasthenia gravis ,t-lymphocytes ,interleukins ,reverse transcriptase polymerase chain reaction ,enzyme-linked immunosorbent assay ,Neurology. Diseases of the nervous system ,RC346-429 - Abstract
Objective To investigate the expression of helper T cell 17 (Th17) cells and associated cytokines in patients with myasthenia gravis (MG) and its correlations to the level of anti-acetylcholine receptor (AChR) antibodies. Methods Thirty patients with MG including 16 patients with ocular myasthenia gravis (OMG) and 14 patients with general myasthenia gravis (GMG) were enrolled in the study from September 2016 to December 2017. Flow cytometry was used to calculate the percentage of circulating Th17 cells in the peripheral blood mononuclear cell (PBMC). Real-time reverse transcription polymerase chain reaction (RT-PCR) was used to examine the expression of interleukin-17A (IL-17A) retinoid-related orphan receptor γ (RORγ) mRNA. Enzyme-linked immunosorbent assay (ELISA) was used to detect IL-17A, IL-6, IL-23 and anti-AChR antibodies. Results The percentage of circulating Th17 cells (t = - 3.312, P = 0.002; t = - 2.286, P = 0.030), the expression of the IL-17A level (t = - 4.282, P = 0.004; t = - 2.788, P = 0.007) and RORγ mRNA (t = 4.408, P = 0.001; t = 1.991, P = 0.049) in GMG and OMG group were higher than those in control group with statistical significance. IL-23 was also higher in the GMG group than that in the control group (t = -2.267, P = 0.031). The percentage of Th17 and level of IL-17A were positively correlated with the anti-AChR antibodies (r = 0.851, P = 0.012; r = 0.743, P = 0.025; respectively). Conclusions The high level of Th17 cells and IL-17A may be closely related to the etiology of MG. It must be verified in the future. DOI:10.3969/j.issn.1672-6731.2019.10.014
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- 2019
6. Characterization of Prismatic Slip in SiC Crystals by Chemical Etching Method
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Shan Shan Hu, Shuai Fang, Ya Fei Liu, Qian Yu Cheng, Hong Yu Peng, Ze Yu Chen, Yu Han Gao, Chao Gao, Balaji Raghothamachar, and Michael Dudley
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Mechanics of Materials ,Mechanical Engineering ,General Materials Science ,Condensed Matter Physics - Abstract
In 4H-silicon carbide crystals, basal plane slip is the predominant deformation mechanism. However, prismatic slip is often observed in single crystals grown by the physical vapor transport method as the diameter expands to 6 inches or larger. Thermal modeling has shown that occurrence of prismatic slip is attributed to increased radial thermal gradients. While X-ray topography can be used to characterize the presence and extent of prismatic slip, the feasibility of using the chemical etching method to assess the extent of prismatic slip in an industrial setting is investigated. The distribution of scallop shaped etch pits oriented along the directions that correspond to prismatic dislocations, correlate well with the results of the thermal model that predicts the occurrence of prismatic slip dislocations. This capability of the etch pit method to characterize prismatic slip can be used to manage radial thermal gradients during PVT growth.
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- 2023
7. Role of Plant-Derived Natural Compounds in Experimental Autoimmune Encephalomyelitis: A Review of the Treatment Potential and Development Strategy
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Yu-Xin Guo, Yuan Zhang, Yu-Han Gao, Si-Ying Deng, Li-Mei Wang, Cui-Qin Li, and Xing Li
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multiple sclerosis ,anti-inflammatory ,BBB ,neuroprotective ,nature product ,Therapeutics. Pharmacology ,RM1-950 - Abstract
Multiple sclerosis (MS) is an autoimmune disease of the central nervous system that is mainly mediated by pathological T-cells. Experimental autoimmune encephalomyelitis (EAE) is a well-known animal model of MS that is used to study the underlying mechanism and offers a theoretical basis for developing a novel therapy for MS. Good therapeutic effects have been observed after the administration of natural compounds and their derivatives as treatments for EAE. However, there has been a severe lag in the research and development of drug mechanisms related to MS. This review examines natural products that have the potential to effectively treat MS. The relevant data were consulted in order to elucidate the regulated mechanisms acting upon EAE by the flavonoids, glycosides, and triterpenoids derived from natural products. In addition, novel technologies such as network pharmacology, molecular docking, and high-throughput screening have been gradually applied in natural product development. The information provided herein can help improve targeting and timeliness for determining the specific mechanisms involved in natural medicine treatment and lay a foundation for further study.
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- 2021
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8. Comfort level discussion for prosthetic sockets with different fabricating processing conditions
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Cheung-Hwa Hsu, Chao-Hui Ou, Wei-Lun Hong, and Yu-Han Gao
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Prosthetic socket ,Interface pressures ,Rapid prototyping ,Gait analysis ,Medical technology ,R855-855.5 - Abstract
Abstract Background In the past, manufacture of prosthetic socket by using traditional handmade method not only consumed research time but also required a special assembly approach. Recently, reverse engineering and rapid prototype technology have grown up explosively, and thus, provide a choice to fabricate prosthetic socket. Methods Application 3D computer aided design and manufacturing (computer-aided design/computer-aided engineering) tools approach the surface shape stump data is digitized and can be easily modified and reused. Collocation investigates gait parameters of prosthetic socket, and interface stress between stump and socket with different processing conditions. Meanwhile, questionnaire was utilized to survey satisfaction rating scale, comfort level, of subjects using this kind of artificial device. Results The main outcome of current research including gait parameters, stress interface and satisfaction rating scale those would be an informative reference for further studies in design and manufacture as well as clinical applications of prosthetic sockets. Conclusions This study found that, regardless of the method used for socket fabrication, most stress was concentrated in tibia end pressure-relief area. This caused discomfort in the area of tibia end to the participant wearing prosthesis. This discomfort was most evident in case when the prosthetic socket was fabricated using RE and RP.
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- 2018
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9. Responses of Soil Abiotic Properties and Microbial Community Structure to 25-Year Cucumber Monoculture in Commercial Greenhouses
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Yu-Han Gao, Xiao-Hong Lu, Rong-Jun Guo, Jian-Jun Hao, Zuo-Qing Miao, Li Yang, and Shi-Dong Li
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monoculture ,rhizosphere microbial community ,soil fertility ,salinization ,cucumber ,Agriculture (General) ,S1-972 - Abstract
Cucumber monoculture could cause soil salinization and acidification, soilborne diseases, and eventually yield loss. However, after a 25-year monoculture in Wafangdian county, Liaoning province, China, cucumber yields have remained satisfactory. In the present study, we investigated how the soil abiotic and biotic properties, in addition to soil rhizosphere microbial community structure in the greenhouses, could still sustain plant growth after such long-term monoculture production. Soil organic carbon, total nitrogen, and available nitrogen were accumulated significantly after monoculture. Such increasing soil fertility was accompanied by increased soil microbial abundance, which reshaped soil microbial community structure. Both bacterial and fungal diversity, including observed and estimated richness, Heip evenness, and Shannon diversity, decreased significantly, while the monoculture period had a greater influence on fungal diversity than on bacterial diversity. Although the high accumulation of soil nutrients increased soil salinity and acidity, the abundance of potential plant pathogenic fungi did not show an obvious increase. These results indicated that maintaining soil abiotic and biotic properties using organic fertilizers and balanced chemical fertilizers, especially improving potassium fertilizer application, could be useful measurements for the sustainable development of greenhouse vegetable production. In addition, appropriate management strategies should be considered to reduce the potential risk of soil salinization.
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- 2021
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10. A targeted extracellular vesicles loaded with montelukast in the treatment of demyelinating diseases
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Yun, Xiao, Yuan, Zhang, Yu-Han, Gao, Zhuo-Hua, Zhao, Jin, He, Rui, Gao, Yu-Xin, Guo, Li-Bin, Wang, and Xing, Li
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Cyclopropanes ,Male ,Receptor, Platelet-Derived Growth Factor alpha ,Biophysics ,Acetates ,In Vitro Techniques ,Sulfides ,Exosomes ,Ligands ,Biochemistry ,Cuprizone ,Extracellular Vesicles ,Mice ,Drug Delivery Systems ,Phagocytosis ,Animals ,Regeneration ,Cell Lineage ,Molecular Biology ,Myelin Sheath ,Neurons ,Oligodendrocyte Precursor Cells ,Stem Cells ,Cell Differentiation ,Myelin Basic Protein ,Cell Biology ,Mice, Inbred C57BL ,Disease Models, Animal ,Oligodendroglia ,Quinolines ,Demyelinating Diseases - Abstract
The main pathological characteristics of demyelinating diseases are central nervous system (CNS) myelin damage, and the differentiation of oligodendrocyte precursor cells is the therapeutic target of myelin repair. Previous studies have found that a large number of platelet-derived growth factor receptor α(PDGFRα) positive oligodendrocyte progenitor cells (OPCs) accumulate in the lesion area of myelin injury, and differentiation is blocked. However, the therapeutic effects of drugs currently used clinically on OPCs differentiation and myelin repair are limited. The main reason is that it is difficult to reach the effective concentration of the drug in the lesion area. Therefore, efficiently delivering into the CNS lesion area is of great significance for the treatment of MS. Natural exosomes have good biocompatibility and are ideal drug carriers. The delivery of drugs to lesion areas can be achieved by giving the exosomes armed targeting ligand. Therefore, in this study, combining exosomes with PDGFA helps them accumulate in OPCs in vitro and in vivo. Further, load montelukast into exosomes to achieve targeted therapy for cuprizone-induced demyelination animal model. The implementation of this research will help provide effective treatments for demyelinating diseases and lay a theoretical foundation for its application in the clinical treatment of different demyelinating diseases.
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- 2022
11. Real time PCR quantification of Sclerotium rolfsii in chilli tissue and soil
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Yu-Han GAO, Wu MIAO, Rong-Jun GUO, and Shi-Dong LI
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chilli root rot ,specific primer pair ,pathogen identification ,population detection ,population determination ,Plant culture ,SB1-1110 - Abstract
Root rot disease caused by Sclerotium rolfsii is one of the important factors limiting chilli production in southern China. Rapid methods of detection and identification of S. rolfsii are required for disease prediction and management. Herein, we adapted a four-step real time quantitative PCR method to detect S. rolfsii using new designed primer pair S301S/S301A. By using this assay, S. rolfsii was detected in ten soil samples and eight plant samples at concentrations as low as 10-7 g mycelium/g soil or plant, both with and without typical symptoms, while tissue-culturing method could only detect the fungus in two samples. This rapid and quantitative method is efficient for detection and determination of S. rolfsii population in soil and plants.
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- 2015
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12. Rhizosphere Inhibition of Cucumber Fusarium Wilt by Different Surfactin- excreting Strains of Bacillus subtilis
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Ke Jia, Yu-Han Gao, Xiao-Qin Huang, Rong-Jun Guo, and Shi-Dong Li
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colonization ,Fusarium oxysporum f. sp. cucumerinum ,real-time PCR ,surfactin ,Plant culture ,SB1-1110 - Abstract
Bacillus subtilis B006 strain effectively suppresses the cucumber fusarium wilt caused by Fusarium oxysporum f. sp. cucumerinum (Foc). The population dynamics of Foc, strain B006 and its surfactin over-producing mutant B841 and surfactin-deficient mutant B1020, in the rhizosphere were determined under greenhouse conditions to elucidate the importance of the lipopeptides excreted by these strains in suppressing Foc. Results showed that B. subtilis strain B006 effectively suppressed the disease in natural soil by 42.9%, five weeks after transplanting, whereas B841 and B1020 suppressed the disease by only 22.6% and 7.1%, respectively. Quantitative PCR assays showed that effective colonization of strain B006 in the rhizosphere suppressed Foc propagation by more than 10 times both in nursery substrate and in field-infected soil. Reduction of Foc population at the cucumber stems in a range of 0.96 log₁₀ ng/g to 2.39 log₁₀ ng/g was attained at the third and the fifth weeks of B006 treatment in nursery substrate. In field-infected soil, all three treatments with B. subtilis suppressed Foc infection, indicated by the reduction of Foc population at a range of 2.91 log₁₀ ng/g to 3.36 log₁₀ ng/g at the stem base, one week after transplanting. This study reveals that the suppression of fusarium wilt disease is affected by the effective colonization of the surfactin-producing B. subtilis strain in the rhizosphere. These results improved our understanding of the biocontrol mechanism of the B. subtilis strain B006 in the natural soil and facilitate its application as biocontrol agent in the field.
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- 2015
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13. Treatment with anacardic acid modulates dendritic cell activation and alleviates the disease development of autoimmune neuroinflammation in mice
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Yu-Han Gao, Yuan Zhang, Yu-Xin Guo, Jia-Qi Wang, Meng-Yuan Gao, Zhuo-Hua Zhao, Rui Gao, Ya-Nan Sun, Li-Bin Wang, and Xing Li
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Mice, Inbred C57BL ,Mice ,Encephalomyelitis, Autoimmune, Experimental ,Spinal Cord ,Neuroinflammatory Diseases ,Biophysics ,Animals ,Cell Biology ,Dendritic Cells ,Molecular Biology ,Biochemistry ,Anacardic Acids - Abstract
Anacardic acid (AA) is a phenolic acid extract found in a number of plants, crops, and fruits. It exhibits a wide range of biological activities. This study displayed that AA effectively alleviated EAE, a classical mouse model of multiple sclerosis. AA administered to the EAE greatly decreased inflammatory cell infiltration to the CNS and protected the myelin integrity in the white matter of the spinal cord. AA could block lipopolysaccharide-induced DC activation. inhibited the polarization of 2D2 mice-derived T cells by inhibiting the DCs activity. Immunoblot results indicated that the phosphorylation of NF-κB is significantly suppressed in AA-treated DCs. This work displayed that AA possessed a potential anti-inflammatory therapeutic effect for the treatment of autoimmune disease.
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- 2022
14. Cholesterol metabolism: Towards a therapeutic approach for multiple sclerosis
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Yu-Han Gao and Xing Li
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Cellular and Molecular Neuroscience ,Cell Biology - Published
- 2023
15. [Role of Imaging in Early Therapeutic Response Assessment for Neuroendocrine Neoplasm with Liver Metastasis:from Anatomical to Functional View]
- Author
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Yu-Han, Gao, Xuan, Wang, and Hua-Dan, Xue
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Diagnostic Imaging ,Neuroendocrine Tumors ,Liver Neoplasms ,Humans ,Neoplasm Metastasis - Abstract
Liver metastasis is not rare during the course of neuroendocrine neoplasms.The methods for treating neuroendocrine neoplasm with liver metastasis(NENLM)are diversifying,which exposes the limitations of the early therapeutic response assessment based on only morphological changes.The emerging imaging biomarkers can sensitively describe changes in response to treatment from the functional level,providing new ideas for the therapeutic response evaluation of NENLM.In this paper,we reviewed the status quo and the latest research progress of imaging assessment for early therapeutic response of NENLM,aiming to provide reference for assessing the response and further exploring the treatment-related biomarkers.
- Published
- 2022
16. Role of Plant-Derived Natural Compounds in Experimental Autoimmune Encephalomyelitis: A Review of the Treatment Potential and Development Strategy
- Author
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Li-Mei Wang, Cuiqin Li, Si-Ying Deng, Yu-Han Gao, Yu-Xin Guo, Yuan Zhang, and Xing Li
- Subjects
0301 basic medicine ,Drug ,media_common.quotation_subject ,Mini Review ,RM1-950 ,multiple sclerosis ,Neuroprotection ,nature product ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Triterpenoid ,medicine ,Pharmacology (medical) ,media_common ,anti-inflammatory ,Autoimmune disease ,Pharmacology ,Natural product ,Mechanism (biology) ,business.industry ,Multiple sclerosis ,Experimental autoimmune encephalomyelitis ,neuroprotective ,medicine.disease ,030104 developmental biology ,chemistry ,Therapeutics. Pharmacology ,business ,Neuroscience ,BBB ,030217 neurology & neurosurgery - Abstract
Multiple sclerosis (MS) is an autoimmune disease of the central nervous system that is mainly mediated by pathological T-cells. Experimental autoimmune encephalomyelitis (EAE) is a well-known animal model of MS that is used to study the underlying mechanism and offers a theoretical basis for developing a novel therapy for MS. Good therapeutic effects have been observed after the administration of natural compounds and their derivatives as treatments for EAE. However, there has been a severe lag in the research and development of drug mechanisms related to MS. This review examines natural products that have the potential to effectively treat MS. The relevant data were consulted in order to elucidate the regulated mechanisms acting upon EAE by the flavonoids, glycosides, and triterpenoids derived from natural products. In addition, novel technologies such as network pharmacology, molecular docking, and high-throughput screening have been gradually applied in natural product development. The information provided herein can help improve targeting and timeliness for determining the specific mechanisms involved in natural medicine treatment and lay a foundation for further study.
- Published
- 2021
17. Responses of Soil Abiotic Properties and Microbial Community Structure to 25-Year Cucumber Monoculture in Commercial Greenhouses
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Jian-Jun Hao, Shi-Dong Li, Li Yang, Rong-Jun Guo, Yu-Han Gao, Zuo-Qing Miao, and Xiao-Hong Lu
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Soil salinity ,rhizosphere microbial community ,Plant Science ,salinization ,03 medical and health sciences ,monoculture ,lcsh:Agriculture (General) ,030304 developmental biology ,Abiotic component ,0303 health sciences ,Rhizosphere ,soil fertility ,04 agricultural and veterinary sciences ,Soil carbon ,lcsh:S1-972 ,Microbial population biology ,Agronomy ,040103 agronomy & agriculture ,0401 agriculture, forestry, and fisheries ,Environmental science ,Species evenness ,Monoculture ,Soil fertility ,Agronomy and Crop Science ,cucumber ,Food Science - Abstract
Cucumber monoculture could cause soil salinization and acidification, soilborne diseases, and eventually yield loss. However, after a 25-year monoculture in Wafangdian county, Liaoning province, China, cucumber yields have remained satisfactory. In the present study, we investigated how the soil abiotic and biotic properties, in addition to soil rhizosphere microbial community structure in the greenhouses, could still sustain plant growth after such long-term monoculture production. Soil organic carbon, total nitrogen, and available nitrogen were accumulated significantly after monoculture. Such increasing soil fertility was accompanied by increased soil microbial abundance, which reshaped soil microbial community structure. Both bacterial and fungal diversity, including observed and estimated richness, Heip evenness, and Shannon diversity, decreased significantly, while the monoculture period had a greater influence on fungal diversity than on bacterial diversity. Although the high accumulation of soil nutrients increased soil salinity and acidity, the abundance of potential plant pathogenic fungi did not show an obvious increase. These results indicated that maintaining soil abiotic and biotic properties using organic fertilizers and balanced chemical fertilizers, especially improving potassium fertilizer application, could be useful measurements for the sustainable development of greenhouse vegetable production. In addition, appropriate management strategies should be considered to reduce the potential risk of soil salinization.
- Published
- 2021
- Full Text
- View/download PDF
18. [Comparison of Plasma Components between Frozen Plasma and Fresh Frozen Plasma]
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Ran-Ran, Qin, Rui-Qin, Hou, Hong-Yan, Yang, Yin-Ping, Cui, Yu-Han, Gao, Wen-Qin, Tian, and Kai-Yan, Liu
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Plasma ,Beijing ,Humans ,Blood Coagulation Tests ,Blood Coagulation ,Blood Coagulation Factors - Abstract
To compare the plasma components of frozen plasma (FP) and fresh frozen plasma (FFP).Twenty samples of FP and 20 samples of FFP from Beijing Red Cross Blood Center were randomly selected. Immediately after plasma melting, 12 plasma components including coagulation factor, fibrinolytic system and anticoagulation protein were detected, including activated partial thromboplastin time (APTT), prothrombin time (PT), coagulation factor Ⅷ (FⅧ) activity, coagulation factor Ⅴ (FⅤ) activity, fibrinogen(FIB) level, ADAMTS-13 activity, von Willebrand factor(vWF) activity, D-dimer (D-dimer, DD), fibrin degradation products (FDP), antithrombin (AT), protein C (PC), and protein S (PS). All these coagulation components between the two types of plasma were compared and analyzed.Compared with FFP, APTT in FP was significantly prolonged(t=3.428, P0.01), and PT was also significantly prolonged(z=-2.140, P0.05), and FⅧ activity was decreased (t=-3.372, P0.01), but all in the reference range, and PS activity was decreased(t=-2.458,P<0.05), there was no statistical difference in the other part between two types of plasma(P0.05).FP can substitute FFP in the treatment of some diseases, although it is lack of some coagulation factors and anticoagulation protein.普通冰冻血浆与新鲜冰冻血浆部分血浆组分的比较.比较普通冰冻血浆(FP)和新鲜冰冻血浆(FFP)中血浆组分的差异.随机选择北京市红十字血液中心提供的FP和FFP各20份,血浆融化后即刻分别检测凝血因子、纤溶系统及抗凝蛋白指标等12种血浆组分,即活化的部分凝血活酶时间(APTT)、凝血酶原时间(PT)、凝血因子Ⅷ(FⅧ)活性、凝血因子Ⅴ(FⅤ)活性、纤维蛋白原(FIB)水平、血管性血友病因子裂解蛋白酶(ADAMTS-13)活性、血管性血友病因子(vWF)活性、D-二聚体(D-dimer, DD)、纤维蛋白降解产物(fibrin degradation products, FDP)、抗凝血酶(antithrombin , AT)、蛋白C(protein C, PC)、蛋白S(protein S, PS),并进行比较分析.与FFP相比,FP中APTT明显延长(t=3.428, P0.01),PT延长(z=-2.140, P0.05),FⅧ 活性明显降低(t=-3.372, P0.01),但均在参考区间内;PS 活性降低(t=-2.458,P<0.05);两种血浆中其余组分的差异无统计学意义(P0.05).与FFP相比,FP缺乏某些凝血因子和抗凝蛋白,但可替代FFP应用于部分疾病的治疗.
- Published
- 2021
19. Additional file 1 of Trends in burden of multidrug-resistant tuberculosis in countries, regions, and worldwide from 1990 to 2017: results from the Global Burden of Disease study
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Ze-Jin Ou, Yu, Dan-Feng, Liang, Yuan-Hao, Wen-Qiao He, Li, Yong-Zhi, Ya-Xian Meng, Xiong, Hu-Sheng, Zhang, Min-Yi, He, Huan, Yu-Han Gao, Wu, Fei, and Chen, Qing
- Abstract
Additional file 1. The supplementary figures and tables of trends in MDR-TB from 1990 to 2017.
- Published
- 2021
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20. Targeting central nervous system extracellular vesicles enhanced triiodothyronine remyelination effect on experimental autoimmune encephalomyelitis
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Yu-Han Gao, Yu-Xin Guo, Jing Tian, Li-Bin Wang, Sheng-Jiao Song, Xiao-Yu Wu, Rui Gao, Yuan Zhang, Xing Li, Wen-Cheng Wu, and Yun Xiao
- Subjects
QH301-705.5 ,Biomedical Engineering ,Article ,Biomaterials ,Multiple sclerosis ,Myelin ,Neuroinflammation ,medicine ,Demyelinating disease ,Biology (General) ,Remyelination ,Materials of engineering and construction. Mechanics of materials ,Experimental autoimmune encephalomyelitis ,business.industry ,Extracellular vesicles ,medicine.disease ,T3 ,Oligodendrocyte ,Neural stem cell ,medicine.anatomical_structure ,Drug delivery ,TA401-492 ,Cancer research ,Systemic administration ,business ,Biotechnology - Abstract
The lack of targeted and high-efficiency drug delivery to the central nervous system (CNS) nidus is the main problem in the treatment of demyelinating disease. Extracellular vesicles (EVs) possess great promise as a drug delivery vector given their advanced features. However, clinical applications are limited because of their inadequate targeting ability and the “dilution effects” after systemic administration. Neural stem cells (NSCs) supply a plentiful source of EVs on account of their extraordinary capacity for self-renewal. Here, we have developed a novel therapeutic system using EVs from modified NSCs with high expressed ligand PDGF-A (EVPs) and achieve local delivery. It has been demonstrated that EVPs greatly enhance the target capability on oligodendrocyte lineage. Moreover, EVPs are used for embedding triiodothyronine (T3), a thyroid hormone that is critical for oligodendrocyte development but has serious side effects when systemically administered. Our results demonstrated that systemic injection of EVPs + T3, versus EVPs or T3 administration individually, markedly alleviated disease development, enhanced oligodendrocyte survival, inhibited myelin damage, and promoted myelin regeneration in the lesions of experimental autoimmune encephalomyelitis mice. Taken together, our findings showed that engineered EVPs possess a remarkable CNS lesion targeting potential that offers a potent therapeutic strategy for CNS demyelinating diseases as well as neuroinflammation., Graphical abstract Schematic representation of EVPs + T3 treatment ameliorated disease development and promoted myelin regeneration.Image 1, Highlights • NSC-derived EV-PDGFA dramatically increased targeting efficiency to the lineage of OLGs and the demyelinated area in the CNS. • EVPs-T3 exert the therapeutic ability in the lesion suppressed the disease development and protected myelin loss. • EVPs-T3 increased numbers of OLGs in the lesion and TEM data evidenced that EVPs-T3 promotes myelin regeneration in vivo.
- Published
- 2020
21. General Steps and Technical Principles of Electric Vehicle(EV) Charging Facility Planning
- Author
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Yu-Han Gao, Mengyao Tian, Chang Diao, Jia-Cheng Sun, Lei Lu, Yong-Cheng Ping, and Hao-Hao Shen
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Computer Science::Robotics ,Mathematical optimization ,business.product_category ,Distribution (number theory) ,Markov chain ,Facility planning ,Hardware_GENERAL ,Simple (abstract algebra) ,Computer science ,Genetic algorithm ,Electric vehicle ,business - Abstract
This paper summarizes the general steps and methods of electric vehicle charging facility planning, and puts forward a simple and feasible model. Markov prediction based on the travel rules of electric vehicle users to obtain the spatiotemporal distribution of charging demand; The minimum comprehensive cost model is established and solved by genetic algorithm. Finally, the technical principles of electric vehicle charging facility planning are summarized.
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- 2020
22. Research on Charging Load Modeling and Influence on Distribution Network of Household Electric Vehicle
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Yong-Cheng Ping, Lei Lu, Chang Diao, Jia-Cheng Sun, Chen-Chen Gou, Jia-Bin Gu, Yu-Han Gao, Hao-Hao Shen, and Shu Jiachi
- Subjects
Distribution system ,Electric power system ,Offset (computer science) ,business.product_category ,Distribution networks ,Hardware_GENERAL ,Computer science ,Load modeling ,Monte Carlo method ,Electric vehicle ,business ,Automotive engineering ,Voltage - Abstract
The impact of large-scale access of electric vehicles on the power distribution network of electric power systems has become increasingly prominent. To solve the problems brought by electric vehicle charging to the distribution system, it is particularly important to predict the charging load of electric vehicles. Based on the travel chain theory, this paper models the charging demand of household electric vehicles. Considering the uncertainty of charging demand in space and time, combined with the Monte Carlo method, taking the typical IEEE33 node power distribution system as an example, this paper simulates the impact of electric vehicle charging load access on the distribution network under different penetration rates and different access modes. The research results show that the charging load of household electric vehicles is usually concentrated in residential areas, and the impact on the overall load characteristics of the distribution network and the node voltage offset is more significant as the penetration rate of electric vehicles increases
- Published
- 2020
23. Additional file 2 of Composition of gut and oropharynx bacterial communities in Rattus norvegicus and Suncus murinus in China
- Author
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Wen-Qiao He, Xiong, Yi-Quan, Ge, Jing, Chen, Yan-Xia, Chen, Xue-Jiao, Xue-Shan Zhong, Ze-Jin Ou, Yu-Han Gao, Cheng, Ming-Ji, Mo, Yun, Wen, Yu-Qi, Qiu, Min, Huo, Shu-Ting, Chen, Shao-Wei, Zheng, Xue-Yan, He, Huan, Li, Yong-Zhi, You, Fang-Fei, Zhang, Min-Yi, and Chen, Qing
- Abstract
Additional file 2: Table S1.The summary of samples collection, grouping and sequencing results.
- Published
- 2020
- Full Text
- View/download PDF
24. Draft Genome Sequence of Bacillus velezensis B6, a Rhizobacterium That Can Control Plant Diseases
- Author
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Rong-jun Guo, Yu-Han Gao, and Shi-dong Li
- Subjects
0301 basic medicine ,Genetics ,Whole genome sequencing ,Strain (biology) ,food and beverages ,Biology ,Genome ,Plant disease ,03 medical and health sciences ,030104 developmental biology ,Bacteriocin ,Prokaryotes ,Molecular Biology ,Gene ,Bacillus velezensis - Abstract
The draft genome of Bacillus velezensis strain B6, a rhizobacterium with good biocontrol performance isolated from soil in China, was sequenced. The assembly comprises 32 scaffolds with a total size of 3.88 Mb. Gene clusters coding either ribosomally encoded bacteriocins or nonribosomally encoded antimicrobial polyketides and lipopeptides in the genome may contribute to plant disease control.
- Published
- 2018
25. An Encoder Used in an Ultra High-Speed Folding and Interpolating ADC
- Author
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Dong Bing Fu, Yong Lu Wang, Ru Zhang Li, Yu Han Gao, and Zheng Ping Zhang
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Comparator ,Computer science ,Quantization (signal processing) ,General Engineering ,Folding (DSP implementation) ,Gray code ,Synchronization (alternating current) ,Sampling (signal processing) ,Hardware_INTEGRATEDCIRCUITS ,Code (cryptography) ,Electronic engineering ,Bit error rate ,Binary code ,Hardware_ARITHMETICANDLOGICSTRUCTURES ,Encoder - Abstract
High speed encoder is the key element of high speed analog-to-digital converter (ADC). Therefor the type of encoder, the type of code, bubble error suppression and bit synchronization must be taken into careful consideration especially for folding and interpolating ADC. To reduce the bubble error which may resulted from the circuit niose, comparator metastability and other interference, the output of quantizer is first encoded with gray code and then converted to binary code. This high speed encoder is verified in the whole time-interleaved ADC with 0.18 Bi-CMOS technology, the whole ADC can achieve a SNR of 45 dB at the sampling rate of 5GHz and input frequency of 495MHz, meanwhile a bit error rate (BER) of less than 10-16 is ensured by this design.
- Published
- 2014
26. An 8-bit 5-Gsample/s Time-Interleaved Analog-to-Digital Converter Used for Optical Communication
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Rong Bin Hu, Yong Lu Wang, Rong Ke Ye, Yu Han Gao, Guang Bin Chen, Zheng Ping Zhang, Can Zhu, and Lei Zhang
- Subjects
Effective number of bits ,Time interleaved ,law ,Computer science ,Hardware_INTEGRATEDCIRCUITS ,General Engineering ,8-bit ,Electronic engineering ,Optical communication ,Analog-to-digital converter ,Successive approximation ADC ,Hardware_ARITHMETICANDLOGICSTRUCTURES ,law.invention - Abstract
In this paper, we present an 8-bit 5 Gsample/s time-interleaved analog-to-digital converter (TI ADC). A 4-phase low jitter clock is designed to control four 1.25 Gsample/s sub-ADCs which is implemented using folding and interpolating architecture. Digital calibration is used to adjust the offset error and gain error of sub-ADCs. Meanwhile, serial peripheral interface (SPI) is adopted to adjust mismatch of gain and sample time between sub-ADCs. The whole TI ADC is designed using a 0.18m SiGe BiCMOS process. The whole ADC has a SNR of about 45 dB at the input frequency of 495 MHZ and an equivalent ENOB of 7.2 bits.
- Published
- 2013
27. [Spatial distribution pattern of soil nitrogen in Huanghuadianzi watershed and related affecting factors]
- Author
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Long, Li, Yun-feng, Yao, Fu-cang, Qin, Yu-han, Gao, and Mei-li, Zhang
- Subjects
China ,Soil ,Spatial Analysis ,Nitrogen ,Altitude ,Agriculture ,Forests ,Grassland - Abstract
This research was conducted in Huanghuadianzi watershed in Aohan, Chifeng, Inner Mongolia. Geostatistic was used to study the spatial distribution of soil nitrogen and their affecting factors. The results showed that the soil nitrogen contents in all layers distributed as an island shape, and the high value areas were mainly distributed in the northwest of the watershed as an obvious fertile island shape, while the low value areas were mainly distributed in the south of the watershed. Nitrogen was mainly concentrated in the surface soil, and its content decreased with the increase of soil depth. The soil nitrogen content at first increased then decreased with the altitude, decreased with the slope, and showed the order of shady slopesemi-shady slopesemi-sunny slopesunny slope in different aspects. The average soil nitrogen contents in different land use types ranked as croplandwoodlandgrassland.
- Published
- 2015
28. Research of Aviation Image Multiplication Based on the Fractal Interpolation Algorithm
- Author
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Zhi Yong An, Tong Pan, Yu Han Gao, and Qiu Shui Yu
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Geometrical optics ,business.industry ,Aviation ,Stairstep interpolation ,General Medicine ,Linear interpolation ,Image (mathematics) ,Fractal ,Multiplication ,Computer vision ,Artificial intelligence ,business ,Mathematics ,Interpolation - Abstract
The resolution of aerial images was low, because the aviation aerospace camera was far from the scenery when shooting. In order to see the object of the aviation images clearly,the aviation images should be enlarged to improve the resolution . As the complexity and irregularity of the aerial images, the texture features is lost and considerable error is caused by using the traditional methods. In this article, the fractal interpolation has been used to enlarge the images, which can not only overcome the shortcoming of the linear interpolation, but also maintain the fine texture features of the original images, which is helpful to obtain higher accuracy than the traditional interpolation.
- Published
- 2010
29. English Semantic Recognition Based on Abstract Meaning Representation and Vector Space Model
- Author
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Yu Han Gao
- Subjects
History ,business.industry ,Computer science ,Representation (arts) ,computer.software_genre ,Experimental research ,Computer Science Applications ,Education ,Focus (linguistics) ,Development (topology) ,Information system ,Vector space model ,Robot ,Artificial intelligence ,business ,computer ,Natural language processing ,Meaning (linguistics) - Abstract
With the development and application of computer science and technology innovation, artificial intelligence industry has achieved sharp development, and its technology has been widely used in many fields, such as natural language processing, robot manufacturing, semantic recognition, information system development, etc. In terms of natural language processing, the recognition and understanding of human language based on artificial intelligence has become the focus of research. And as semantic recognition and processing play an important part of natural language processing, they have achieved satisfactory results in the Abstract Meaning Representation (AMR) and Vector Space Model (VSM) development and application. Based on this, this paper takes the English recognition as the research object, adopts the scientific research methods such as literature analysis, practice investigation and experimental research, further analyzes English semantic recognition based on AMR and VSM from the aspects of semantic recognition difficulty, principles of AMR and VSM and its application in English recognition, as well as the practical applications. The author hopes to provide references for future research.
- Published
- 2018
30. Power distribution network design of current-steering, nyquist rate DA converters.
- Author
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Yu-Han Gao, Lin-Tao Liu, and Ru-Zhang Li
- Published
- 2011
- Full Text
- View/download PDF
31. Chip and package co-design for high performance mixed IC.
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Yu-Han Gao, Lin-Tao Liu, Liang Chen, Xing-Fa Huang, Ru-Zhang Li, and Ming-Yuan Xu
- Published
- 2010
- Full Text
- View/download PDF
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