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Along with long-term evolution, the plant cell wall generates lignocellulose and other anti-degradation barriers to confront hydrolysis by fungi. Lytic polysaccharide monooxygenase (LPMO) is a newly defined oxidase in lignocellulosic degradation systems that significantly fuels hydrolysis. LPMO accepts electrons from wide sources, such as cellobiose dehydrogenase (CDH), glucose-methanol-choline (GMC) oxidoreductases, and small phenols. In addition, the extracellular cometabolic network formed by cosubstrates improves the degradation efficiency, forming a stable and efficient lignocellulose degradation system. In recent years, using structural proteomics to explore the internal structure and the complex redox system of LPMOs has become a research hotspot. In this review, the diversity of LPMOs, catalytic domains, carbohydrate binding modules, direct electron transfer with CDH, cosubstrates, and degradation networks of LPMOs are explored, which can provide a systematic reference for the application of lignocellulosic degradation systems in industrial approaches.

The southeastern rock base sea area is the most abundant wind resource area, and it is also the mainstream construction site of offshore wind farms (OWFs) in China. The weathered residual soil is the main seabed component in the rock base area, which is the important bearing stratum of the offshore wind turbine foundation. Previous studies on the mechanical properties of seabed materials and bearing characteristics of the pile foundations in OWFs have mainly focused on the submarine soil-based seabed, resulting in a lack of direct reference for the construction of offshore wind power in the rocky seabed. Therefore, the mechanical properties of weathered residual soil and the bearing behaviors of monopile foundations are mainly investigated in this study. Firstly, dynamic triaxial tests are conducted on the weathered residual soil, and experiments analyze insight into the evolution law of the hysteresis curve, cumulative strain, and stiffness attenuation. Then, the horizontal loading behaviors of monopile foundations in residual soil are analyzed by numerical simulations; more critically, the service performances under wind and wave coupling loads are evaluated, which provide a direct theoretical basis for the construction and design of offshore wind turbine foundations in rock base seabeds. [ABSTRACT FROM AUTHOR]

Diabetes management applications effectively help patients monitor blood glucose levels and adjust diet and exercise plans. However, most diabetes management apps, including the SugarShift application developed in 2023, use generalized designs that often fail to consider the unique cognitive and physiological characteristics of elderly users, thereby impacting their acceptance and usage. This paper proposes an enhancement for the SugarShift application by introducing a mode specifically designed for elderly users. This proposed enhancement aims to directly involve elderly patients so as to meet their needs better through a participatory design approach. A heuristic evaluation conducted with six experts identified 126 usability issues and 179 heuristic violations in the current version, which has led to the recommendation to develop optimization strategies tailored for elderly users. Subsequent usability testing with 28 elderly patients and six experts emphasized the importance of interface simplicity, logical operation, and interaction quality in enhancing user experience. These factors improve usability, facilitate information processing, and reduce cognitive load. Despite the overall usability of SUS scores, significant challenges still need to be addressed in information display, system feedback, and user interaction. Recommendations for future enhancements include simplifying data entry and presentation, improving readability, and providing timely feedback to enhance usability and user satisfaction, ultimately increasing user retention. [ABSTRACT FROM AUTHOR]

The investigation of the interplay between complex coacervate microdroplets and amphiphilic molecules offers valuable insights into the processes of prebiotic compartmentalization on the early Earth and presents a promising avenue for future advancements in biotechnology. Herein, the interaction between complex coacervate microdroplets and amphiphilic molecule (decanoic acid) is systematically investigated by varying charge strengths of negatively charged polyelectrolytes (DNA and PAA) and positively charged polyelectrolytes (PDDA and DEAE‐Dextran). It is found that the interaction between amphiphilic molecule and complex coacervate microdroplets depended on the delicate balance between the interaction between decanoic acid and polyelectrolyte and the interaction between two polyelectrolytes. The different spatial distribution of amphiphilic molecule can result in differences in the internal microenvironment, which can further alter the uptake or exclusion of small molecules and biomolecules with different charges and polarities and functional biological process. [ABSTRACT FROM AUTHOR]

The common practice of defining operational settings for Heating, Ventilation and Air Conditioning (HVAC) systems in buildings is to use fixed set points, which assume occupants have same and static comfort requirements. However, thermal comfort varies from person to person and also changes due to climatic variations or acclimation, making it dynamic. In addition, thermal comfort in transient conditions are different from the steady state conditions, which makes the prediction of thermal comfort more difficult. Thus, thermal comfort has to be monitored over time. In this paper, we present a novel infrared thermography based technique to monitor an individual’s thermoregulation performance and thermal comfort levels by measuring the skin temperature on several points on human face, which has a high density of blood vessels and is not usually covered by clothing. Unlike other methods, our method requires no continuous user input or interaction. Our results demonstrate that the monitored facial points behave differently under the heat and cold stresses and it can be explained based on the underlying vascular territories. We define two heuristics to describe the thermoneutral zone based on the observed behaviors and estimate thermal comfort for individuals with 95% confidence level. Considerable variations are observed in the thermoregulation performance and uncomfortably cool conditions metrics between the males and females. Females’ thermoregulation system responses are less sensitive to the perception of warm conditions. However, similar behaviors are observed for uncomfortably cool conditions across genders.

Combining various sources to create a complementary system plays a key role in utilizing clean energy sources economically and mitigating air pollution during the heating season in Northern China. However, there is a lack of unified and reasonable design methods for such systems, resulting in the excessive capacity of equipment and the waste of energy. In this work, a unified design method is proposed to solve this problem. A generalized structure and its mathematical model are firstly established, enabling transient simulations on the TRNSYS platform. Then, a preliminary screening criterion for the system composition a general operation strategy is proposed. Finally, the system configuration is optimized by using the genetic algorithm. The method is successfully applied in a demonstration project in China. The results show that the coupling system consisting of a biomass boiler (384 kW), an air-source heat pump (430 kW) and a ground-source heat pump (369 kW) is the most economical, and the annual cost is 26.7% lower than that of a single-equipment system. Additionally, the sensitive factors that strongly affect the optimization results are explored. The establishment of the generalized structure and its mathematical model enables the quick calculation and convenient comparison of various schemes, and simplifies the complicated optimization problem of the capacity optimization of each piece of equipment. The proposed design method can reduce the annual cost to a minimum value, and thus it provides a theoretical basis for the large-scale application of clean energy sources for heating. [ABSTRACT FROM AUTHOR]