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Mutations in the cystic fibrosis transmembrane conductance regulator (CFTR) gene cause cystic fibrosis (CF), a multiorgan disease that is characterized by diverse metabolic defects. However, other than specific CFTR mutations, the factors that influence disease progression and severity remain poorly understood. Aberrant metabolite levels have been reported, but whether CFTR loss itself or secondary abnormalities (infection, inflammation, malnutrition, and various treatments) drive metabolic defects is uncertain. Here, we implemented comprehensive arteriovenous metabolomics in newborn CF pigs, and the results revealed CFTR as a bona fide regulator of metabolism. CFTR loss impaired metabolite exchange across organs, including disruption of lung uptake of fatty acids, yet enhancement of uptake of arachidonic acid, a precursor of proinflammatory cytokines. CFTR loss also impaired kidney reabsorption of amino acids and lactate and abolished renal glucose homeostasis. These and additional unexpected metabolic defects prior to disease manifestations reveal a fundamental role for CFTR in controlling multiorgan metabolism. Such discovery informs a basic understanding of CF, provides a foundation for future investigation, and has implications for developing therapies targeting only a single tissue., Introduction Cystic fibrosis (CF) is an autosomal recessive disease caused by loss-of-function mutations in the gene encoding the CF transmembrane conductance regulator (CFTR) anion channel (1-4). The clinical phenotype is [...]

Understanding the coupled continuum pipe‐flow framework for modeling contaminant transport in karst systems is critical for protecting water resources therein. This study simulated point and non‐point source nitrate concentration in a karst spring and investigated the results generated from the flow and transport model with and without discrete conduit flow. CFPv2 and CMT3D models were integrated to address the changes in nitrate concentration at a monthly scale in a karst spring, and the results were compared with that from an equivalent porous media (EPM) model with high‐hydraulic conductivity (K) zones set in the main karstified area to represent conduits. The results show that the CFPv2+CMT3D model is able to describe well the recession of nitrate concentration in spring discharge, and the relatively larger deviation (slower nitrate recession) from the observed trend for the EPM model is probably a result of the limitation of utilizing high hydraulic conductivity cells to represent conduit. Moreover, simulated hydraulic heads in poorly karstified areas from the two models both show slight differences from the observations (the head RMSE values of calibration/validation for CFPv2 and MODFLOW models are 0.16 m/0.25 m and 0.26 m/0.17 m, respectively), indicating the inclusion of conduits may not affect the simulation considerably, and the lower the proportion of karstic area, the slight effects brought from the inclusion of conduits in the model. For highly karstified areas, the CFPv2+CMT3D model could provide more accurate results (head RMSE of calibration/validation for CFPv2 and MODFLOW are 0.22 m/0.06 and 0.52 m/0.47 m, respectively), showing the coupled continuum pipe‐flow framework may be more appropriate for applying to highly and maturely karstified areas where the variations in the behavior of flow and contaminant transport are more affected by turbulent flow regime.

Objective To investigate the effect of quality control circle (QCC)activity on standard prevention of venous thromboembolism (VTE)among hospitalized pregnant women. Methods Totally 88 hospitalized pregnant women were recruited in this study, in which 44 cases were hospitalized before implementation of QCC activity and another 44 cases were hospitalized after implementation of QCC activity. The rate of standard VTE prevention, VTE awareness, compliance rate of function exercise, rate of sufficient daily intake of water and satisfaction with nursing service were measures before and after implementation of QCC activity. Results The rate of standard VTE prevention increased from 54. 55%(24/44)before implementation of QCC activity to 81. 82%(36/44)after implementation of QCC activity. Higher score of VTE knowledge assessment, rate of function exercise and sufficient daily intake of water were observed after implementation of QCC activity compared with those before implementation of QCC activity(P＜0. 01). Nursing satisfaction rate was increased significantly after implementation of QCC activity(P＜0. 05). Conclusion The QCC activity is potentially effective to improve hospitalized pregnant women’s awareness and behavior of standard VTE prevention, therefore reducing the risk of VTE during pregnancy. (目的 探讨品管圈活动在提高住院孕产妇静脉血栓栓塞(VTE)规范预防率中的应用效果。方法 选取 2021年1月—6月妇产科收治的88位孕产妇为研究对象, 其中实施品管圈活动前收治44例, 活动开展后收治44例。比较活动前和活动后孕产妇VTE规范预防率、VTE相关知识掌握情况、功能锻炼执行率、每日充足饮水达标率及护理满意度。结果 住院孕产妇VTE规范预防率由活动前的54. 55%(24/44)提高至活动后的81. 82%(36/44)。品管圈活动后, 孕产妇VTE知识掌握情况、功能锻炼执行率、每日充足饮水达标率均高于活动前(P＜0. 01); 住院孕产妇对护理满意度高于活动前(P＜0. 05)。结论 对住院孕产妇开展品管圈活动, 可提升其对VTE相关知识的认知程度, 提高VTE规范预防率, 对降低VTE 发生风险具有积极意义。)