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18 results on '"Yu, Jiangliu"'

11. Biosynthesis of gold nanoparticles by the extreme bacterium Deinococcus radiodurans and an evaluation of their antibacterial properties

Author

Li,Jiulong, Li,Qinghao, Ma,Xiaoqiong, Tian,Bing, Li,Tao, Yu,Jiangliu, Dai,Shang, Weng,Yulan, and Hua,Yuejin

Subjects
International Journal of Nanomedicine
Abstract

Jiulong Li,1,* Qinghao Li,1,* Xiaoqiong Ma,2,* Bing Tian,1 Tao Li,1 Jiangliu Yu,1 Shang Dai,1 Yulan Weng,1 Yuejin Hua1 1Key Laboratory for Nuclear-Agricultural Sciences of Chinese Ministry of Agriculture and Zhejiang Province, Institute of Nuclear-Agricultural Sciences, Zhejiang University, 2Central Laboratory, The First Affiliated Hospital of Zhejiang Chinese Medical University, Hangzhou,China *These authors contributed equally to this work Abstract: Deinococcus radiodurans is an extreme bacterium known for its high resistance to stresses including radiation and oxidants. The ability of D. radiodurans to reduce Au(III) and biosynthesize gold nanoparticles (AuNPs) was investigated in aqueous solution by ultraviolet and visible (UV/Vis) absorption spectroscopy, electron microscopy, X-ray diffraction (XRD), dynamic light scattering (DLS), Fourier transform infrared spectroscopy (FTIR) and X-ray photoelectron spectroscopy (XPS). D. radiodurans efficiently synthesized AuNPs from 1mM Au(III) solution in 8h. The AuNPs were of spherical, triangular and irregular shapes with an average size of 43.75nm and a polydispersity index of 0.23 as measured by DLS. AuNPs were distributed in the cell envelope, across the cytosol and in the extracellular space. XRD analysis confirmed the crystallite nature of the AuNPs from the cell supernatant. Data from the FTIR and XPS showed that upon binding to proteins or compounds through interactions with carboxyl, amine, phospho and hydroxyl groups, Au(III) may be reduced to Au(I), and further reduced to Au(0) with the capping groups to stabilize the AuNPs. Biosynthesis of AuNPs was optimized with respect to the initial concentration of gold salt, bacterial growth period, solution pH and temperature. The purified AuNPs exhibited significant antibacterial activity against both Gram-negative (Escherichia coli) and Gram-positive (Staphylococcus aureus) bacteria by damaging their cytoplasmic membrane. Therefore, the extreme bacterium D. radiodurans can be used as a novel bacterial candidate for efficient biosynthesis of AuNPs, which exhibited potential in biomedical application as an antibacterial agent. Keywords: Deinococcus radiodurans, gold nanoparticles, gold ions, reduction, antibacterial&nbsp

Published
2016

17. DqsIR quorum sensing-mediated gene regulation of the extremophilic bacterium D einococcus radiodurans in response to oxidative stress.

Author

Lin, Lin, Dai, Shang, Tian, Bing, Li, Tao, Yu, Jiangliu, Liu, Chengzhi, Wang, Liangyan, Xu, Hong, Zhao, Ye, and Hua, Yuejin

Subjects
QUORUM sensing, DEINOCOCCUS radiodurans, ENZYMES, GENE expression, MICROBIOLOGY of extreme environments
Abstract

Here, we show that AHLs can be employed by Deinococcus radiodurans, which belongs to the unique phylum Deinococcus-Thermus and is known for its cellular resistance to environmental stresses. An AHL-mediated quorum-sensing system (DqsI/DqsR) was identified in D. radiodurans. We found that under non-stress conditions, the AHL level was 'shielded' by quorum quenching enzymes, whereas AHLs accumulated when D. radiodurans was exposed to oxidative stress. Upon exposure to H2O2, AHL synthetic enzymes (DqsI) were immediately induced, while the expression of quorum-quenching enzymes began to increase approximately 30 min after exposure to H2O2, as shown by time-course analyses of gene expression. Both dqsI mutant (DMDqsI) and dqsR mutant (MDqsR) were more sensitive to oxidative stress compared with the wild-type strain. Exogenous AHLs (5 μM) could completely restore the survival fraction of DMDqsI under oxidative stress. RNA-seq analysis showed that a number of genes involved in stress-response, cellular cleansing, and DNA repair had altered transcriptional levels in MDqsR. The DqsR, acting as a regulator of quorum sensing, controls gene expression along with AHLs. Hence, the DqsIR-mediated quorum sensing that mediates gene regulation is an adaptive strategy for D. radiodurans in response to oxidative stresses and is conserved in the extremophilic Deinococcus bacteria. [ABSTRACT FROM AUTHOR]

Published
2016
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18. [Screening of specific miRNA in early gastric cancer].

Author

Han J, Yu J, and Ling Z

Subjects
Down-Regulation, Humans, Oligonucleotide Array Sequence Analysis, Up-Regulation, Gene Expression Regulation, Neoplastic, MicroRNAs genetics, Stomach Neoplasms genetics
Abstract

Objective: To examine the micro-RNA (mirna) expression profile in tissues of early gastric cancer and to screen the specific mirna associated with gastric cancer., Methods: Gene chip technology was used to detect the expression of mirna in early gastric cancer tissues and adjacent normal tissues., Results: Compared to adjacent normal tissues, a total of 36 mirnas were down-regulated, such as mir-9-1, mir-103 and mir-141, while 12 mirnas were up-regulated, such as mir-196a, mir-142-3p and mir-25, etc., Conclusion: Abnormal mirna expression level in early gastric cancer tissues may be associated to the development of gastric cancer.

Published
2014

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