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Your search keyword '"Yousefi, Yasamin"' showing total 14 results
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14 results on '"Yousefi, Yasamin"'

6. Enhancing Temozolomide (TMZ) chemosensitivity using CRISPR-dCas9-mediated downregulation of O6-methylguanine DNA methyltransferase (MGMT).

Author

Yousefi, Yasamin, Nejati, Reza, Eslahi, Atiye, Alizadeh, Farzaneh, Farrokhi, Shima, Asoodeh, Ahmad, and Mojarrad, Majid

Abstract

Purpose: Glioblastoma (GBM) stands out as the most prevalent and aggressive intracranial tumor, notorious for its poor prognosis. The current standard-of-care for GBM patients involves surgical resection followed by radiotherapy, combined with concurrent and adjuvant chemotherapy using Temozolomide (TMZ). The effectiveness of TMZ primarily relies on the activity of O6-methylguanine DNA methyltransferase (MGMT), which removes alkyl adducts from the O6 position of guanine at the DNA level, thereby counteracting the toxic effects of TMZ. Method: In this study, we employed fusions of catalytically-inactive Cas9 (dCas9) to DNA methyltransferases (dCas9-DNMT3A) to selectively downregulation MGMT transcription by inducing methylation at MGMT promoter and K-M enhancer. Result: Our findings demonstrate a significant reduction in MGMT expression, leading to intensified TMZ sensitivity in the HEK293T cell line. Conclusion: This study serves as a proof of concept for the utilization of CRISPR-based gene suppression to overcome TMZ resistance and enhance the lethal effect of TMZ in glioblastoma tumor cells. [ABSTRACT FROM AUTHOR]

Published
2024
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13. Periodontal Abscess

Author

Yousefi Y, Meldrum J, and Jan AH

Abstract

While less common than an endodontic abscess, a periodontal abscess is the third most frequent dental emergency requiring immediate intervention due to their rapid onset of pain. A periodontal abscess is described as a localized accumulation of pus within the gingival wall of a periodontal pocket. More prevalent in patients with previous periodontal pockets, it develops rapidly, destroying periodontal tissues and depicting clear symptoms. If the tooth is associated with multiple abscesses, it may have a “hopeless prognosis.” Two etiologies explain the development of this entity: periodontitis-related and non-periodontitis-related. Abscesses of periodontitis-related origin usually appear as an exacerbation of the untreated periodontal disease or during periodontal treatment. Abscesses of non-periodontitis-related origin frequently develop due to the impaction of foreign objects, such as a piece of dental floss, or abnormalities of the root anatomy. The treatment of periodontal abscesses includes drainage, mechanical debridement, and mouth rinses, reserving antibiotic therapy for some cases., (Copyright © 2022, StatPearls Publishing LLC.)

Published
2022

14. Maxillary Fracture

Author

Meldrum J, Yousefi Y, and Jenzer AC

Abstract

Maxillary fractures are one of the most common emergencies presenting in the acute setting [1]. Due to the complex anatomy within this region and the proximity to vital structures, including the brain, early diagnosis and precise treatment planning are of paramount importance. Numerous treatment methods are well-practiced globally, and these aim to restore the patient’s quality of life., (Copyright © 2021, StatPearls Publishing LLC.)

Published
2021

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