1. Evidence of a Pluripotent Human Embryonic Stem Cell Line Derived from a Cloned Blastocyst
- Author
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Byeong Chun Lee, Sun Jong Kim, Sung Keun Kang, Jong Hyuk Park, Ky Young Park, Curie Ahn, Shin Yong Moon, Woo Suk Hwang, Jung Hye Hwang, Eu Gene Lee, Jose B. Cibelli, Eul Soon Park, Hyun Yong Jeon, Ja Min Koo, and Young June Ryu
- Subjects
Male ,Pluripotent Stem Cells ,KOSR ,Nuclear Transfer Techniques ,Cloning, Organism ,Parthenogenesis ,Mice, SCID ,Embryoid body ,Biology ,Cell Line ,Genomic Imprinting ,Mice ,Ovarian Follicle ,Testicular Neoplasms ,Culture Techniques ,Animals ,Humans ,Cell potency ,reproductive and urinary physiology ,Genetics ,Multidisciplinary ,Oocyte Donation ,Reverse Transcriptase Polymerase Chain Reaction ,Teratoma ,Cell Differentiation ,Embryo, Mammalian ,DNA Fingerprinting ,Embryonic stem cell ,Culture Media ,Cell biology ,Blastocyst ,Tandem Repeat Sequences ,Karyotyping ,Somatic cell nuclear transfer ,Female ,Stem cell ,Biomarkers ,Adult stem cell ,Human embryonic stem cell line - Abstract
Somatic cell nuclear transfer (SCNT) technology has recently been used to generate animals with a common genetic composition. In this study, we report the derivation of a pluripotent embryonic stem (ES) cell line (SCNT-hES-1) from a cloned human blastocyst. The SCNT-hES-1 cells displayed typical ES cell morphology and cell surface markers and were capable of differentiating into embryoid bodies in vitro and of forming teratomas in vivo containing cell derivatives from all three embryonic germ layers in severe combined immunodeficient mice. After continuous proliferation for more than 70 passages, SCNT-hES-1 cells maintained normal karyotypes and were genetically identical to the somatic nuclear donor cells. Although we cannot completely exclude the possibility that the cells had a parthenogenetic origin, imprinting analyses support a SCNT origin of the derived human ES cells.
- Published
- 2004
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