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2. Technical note: improved ancient DNA purification for PCR using ion-exchange columns

Author

Ae Ja Park, Ki-Won Park, Ariunaa Togloom, Dashtseveg Tumen, Gavaachimed Lkhagvasuren, Kwon-Jong Yoo, Jee-Hye Choi, Keun-Cheol Kim, Kwang-Ho Lee, Maengseok Noh, Kijeong Kim, Eunhee Jeon, Jae Hyun Kim, Minsoo Lee, Kyung Yong Kim, and Youn-Ock Cho

Subjects
Mitochondrial DNA, Chromatography, Ion exchange, Extraction (chemistry), DNA, Biology, Chromatography, Ion Exchange, Molecular biology, DNA extraction, Polymerase Chain Reaction, Bone and Bones, law.invention, chemistry.chemical_compound, Ancient DNA, chemistry, law, Anthropology, Forensic Anthropology, Humans, Anatomy, Amelogenin, Polymerase chain reaction
Abstract

A novel method of ancient DNA (aDNA) purification was developed using ion-exchange columns to improve PCR-amplifiable DNA extraction from ancient bone samples. Thirteen PCR-resistant ancient bone samples aged 500-3,300 years were tested to extract aDNA using a recently reported, silica-based aDNA extraction method and an ion-exchange column method for the further purification. The PCR success rates of the aDNA extracts were evaluated for the amplification ability of the fragments of mitochondrial DNA, a high-copy DNA, and amelogenin, a low-copy DNA. The results demonstrate that the further purification of silica-based aDNA extracts using ion-exchange columns considerably improved PCR amplification. We suggest that the ion-exchange column-based method will be useful for the improvement of PCR-amplifiable aDNA extraction, particularly from the poorly preserved, PCR-resistant, ancient samples.

Published
2008

3. Development of Ancient DNA Isolation Method for Improved PCR Amplification

Author

Youn Ock Cho, Kyung Yong Kim, Minsoo Lee, Gavaachimed Lkhagvasuren, Eun Hee Jeon, Jae Hyun Kim, Ki Won Park, Ae Ja Park, Kijeong Kim, Kwang Ho Lee, Keun Cheol Kim, Jee Hye Choi, Ariunaa Togloom, Maengseok Noh, Kwon Jong Yoo, Dashtseveg Tumen, and Jong Dae Kim

Subjects
Polymerase chain reaction optimization, Ancient DNA, law, Primer dimer, Multiple displacement amplification, Biology, Ligase chain reaction, Polymerase cycling assembly, DNA extraction, Molecular biology, Polymerase chain reaction, law.invention
Published
2007
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4. Comparison between Morphological Sex and Genotype Sex of Uzbekistan Ancient Bones Using Improved Amelogenin PCR Amplication Method

Author

Ae Ja Park, Kwang Ho Lee, Gavaachimed Lkhagvasuren, Jee Hye Choi, Samardin Mustafokulov, Jong Dae Kim, Eunhee Jeon, Ki Won Park, Jae Hyun Kim, Ariunaa Togloom, Kijeong Kim, Keun Cheol Kim, Kwon Jong Yoo, Na Yung Min, Youn Ock Cho, Kyung Yong Kim, Sang In Chung, Minsoo Lee, Maengseok Noh, and Won Bok Lee

Subjects
Genetics, Ancient DNA, Genotype, Amelogenin, Biology
Published
2007
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5. Technical Note: Improved Ancient DNA Purification for PCR Using Ion-Exchange Columns.

Author

Kijeong Kim, Kyung-Yong Kim, Eunhee Jeon, Togloom, Ariunaa, Youn-Ock Cho, Min-Soo Lee, Lkhagvasuren, Gavaachimed, Jee-Hye Choi, Tumen, Dashtseveg, Ae Ja Park, Keun-Cheol Kim, Ki-Won Park, Jae-Hyun Kim, Maengseok Noh, Kwon-Jong Yoo, and Kwang-Ho Lee

Subjects
DNA, POLYMERASE chain reaction, BONES, GENE amplification, SILICA, IONS
Abstract

The article reports on ion-exchange columns used for the improved ancient deoxyribonucleic acid (aDNA) purification for polymerase chain reaction (PCR). Thirteen PCR-resistant ancient bone samples were tested to extract aDNA using a silica-based method and an ion-exchange column method to prove aDNA purification's effectivity on PCR-amplifiable DNA extraction. The results show that further purification of silica-based aDNA extracts using ion-exchange columns improved PCR amplification.

Published
2008
Full Text
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