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This study is the first to report the presence of PCV4 in dogs worldwide, and the first complete genome sequence was obtained from a dog affected with diarrhea. Three genotypes of PCV4 strains (PCV4a, PCV4b, and PCV4c) were determined, as supported by specific amino acid markers (V239L for open reading frame 1 [ORF1] and N27S R28G and M212L for ORF2).

Two new triterpenoid saponins named lonimacranthoide IV ( 1 ) and lonimacranthoide V ( 2 ) were isolated from the flower buds of Lonicera macranthoides Hand.-Mazz. (Caprifoliaceae). They have hederagenin as aglycone. Lonimacranthoide IV ( 1 ) is a rare chlorogenic acid ester acylated on the C-23 of hederagenin. Lonimacranthoide IV is a new sulfated triterpenoid saponin. The structures of the saponins were established by chemical and spectral methods.

To study the chemical constituents of Suaeda glauca.The chemical constituents were isolated and purified with several separation and purification techniques. Their structures were identified by physicochemical properties and various spectroscopic methods.Ten compounds were isolated from the ethyl acetate fraction as lignoceric acid (1), β-amyrin-n-nonyl ether(2), β-sitosterol(3), β-daucosterol(4), quercetin(5), luteolin(6), luteolin-7-O-β-D-glucoside(7), isorhamnetin(8), scopoletin (9) and stigmasterol(10).Compounds 1, 2, 6, 7, 8, 9 and 10 are isolated from Suaeda genus for the first time and compounds 3 - 5 are isolated from this plant for the first time.

To study chemical constituents of the roots of Lonicera macranthoides.The chemical constituents were isolated and purified by means of several chromatographic techniques and their structures were elucidated by spectroscopic methods.Seven compounds were isolated and identified as ribenol (1), excoecarin C (2), 18-hydroxy-13-epi-manoyloxide (3), asiatic acid (4), oleanolic acid (5), β-sitosterol (6) and β-daucosterol (7).Compounds 1-4 are obtained from this genus for the first time. Compound 5 is obtained from this plant for the first time. All the compounds are found from the roots of Lonicera mac- ranthoides for the first time.

A new polyacetylene glycoside, (1,3Z,11E)-tridecatriene-7,9-diyne-5-hydroxyl-6-O-β- d -glucopyranoside, was isolated from the rhizomes of Atractylodes lancea (Thunb.) DC. Its structure was elucidated on the basis of spectral analysis.

To study the water-soluble chemical constituents of the flower buds of Lonicera macranthoides.The chemical constituents were isolated and purified by means of chromatographic techniques and their structures were elucidated by spectroscopic methods.Six compounds were isolated and identified as trans-linalool-3,7-oxide-6-O-beta-D-glucopyranoside(1), secol oganoside(2), secoxyloganin(3),chlorogenic acid(4), caffeic acid(5), sucrose(6).Compound 1 is isolated from nature as a single compound for the first time, compounds 2-3 are isolated from this plant for the first time.

To study the chemical constituents of the Bulbs of Lycoris longituba.The chemical constituents were isolated and purified by means of chromatographic techniques and their structures were elucidated by spectroscopic methods.Six compounds were isolated and identified as Crinine (1), Narciclasine (2), Squamigine (3), 11-Hydroxyvittatine (4), Pancracine (5), Lycorine (6).Compounds 1 - 6 are isolated from this plant for the first time.

To study the chemical constituents of Salicornia europaea.The chemical constituents were isolated and purified by means of several chromatographic techniques and their structures were elucidated by spectroscopic methods.Five compounds were isolated and identified as syringaresinol 4-O-beta-D-glucopyranoside(1), icariside B2(2), erythro-1-(4-O-beta-D-glucopyranosyl-3,5-dimethyoxyphenyl)-2-syringaresinoxyl-propane-1,3-diol(3), longifloroside B(4), 7-O-beta-D-glucopyranosyl-6-methoxychromone(5).Compounds 1-4 are obtained from this genus for the first time.

To study the chemical constituents of the aerial part of Bupleurum chinense.Dried aerial parts of Bupleurum chinense were extracted with EtOH, the chemical constituents in the extract were isolated and their structures were identified by physical and chemical properties and spectral analysis.Five compounds were obtained and identified as 1-O-caffeoylglycerol (1), esculetin (2), scopoletin (3), kaempferol-3-O-beta-D-rutinoside (4) and alpha-spinastero (5).Compounds 1-3 are obtained from Bupleurum chinense for the first time.