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The highest mortality rate among cancer patients is lung cancer. A large proportion of cancer patients are lung cancer patients. The incidence rate of lung cancer is the highest in the world. In recent years, long-chain non-coding RNA, or LncRNA, has become more and more closely related to cancer and has gradually attracted the attention of many cancer researchers. LncRNA is the proto-oncogene of cancer. We can find out the cause of cancer and put forward effective methods to inhibit the occurrence of cancer by studying LncRNA. This study aimed to investigate the effect of noncoding long-chain RNA16 on the proliferation and molecular mechanism of lung cancer cells. During the operation, the distance between the excised lung cancer block and the adjacent tissue is 2cm from the corresponding lung cancer block. Then, the total RNA in lung cancer cells and tissues is extracted with a Trizole reagent. Then, the expression profile of LncRNA in three cases of lung cancer and the corresponding adjacent tissue is identified by RNA chip technology. The LncRNA related to proliferation and the expression difference is significant through bioinformatics. The conclusion was that the A549 and H1299 groups of shLncRNA16 could significantly reduce the incidence by 64% and 40% percentage points compared with the experimental group, which indicates that LncRNA16 can be used as a potential treatment target for patients.

Objective: To investigate the feasibility of bacterial cellulose patch to repair and reconstruct rabbit tracheal defect, so as to lay the foundation for the development of bacterial cellulose artificial trachea., Methods: Thirty adult rabbits (weighing, 2.5-3.5 kg) were selected to establish full-thickness defect of the cervical trachea (1.0 cm×0.6 cm in size) which involved the anterior and side walls and 2-3 rings. Defect was repaired with sheet bacterial cellulose (1.2?cm in diameter and 6 layers) in experimental group ( n =15), and with autologous cervical fascia in control group ( n =15). The general condition of the animals was observed after operation; after 4, 8, and 12 weeks, the samples were obtained for measuring the tracheal stenosis degree, counting new microvessels, and observing lumen reconstruction situation by scanning electron microscope., Results: Three rabbits died of infection, tracheal stenosis, or asphyxia caused by airway secretions retention in 2 groups respectively, and the other rabbits survived to the end of experiment. Gross observation showed that reconstruction materials gradually adhered?to?adjacent?tissue and were enwrapped?by connective tissue, small blood vessels like nutrient vessel were seen in 2 groups; no significant difference was found in the tracheal lumen stenosis degree between 2 groups ( P > 0.05). Histological observation showed that the continuous growth of mucosal epithelial cells was observed near patch and anastomosis site with time, and cell morphology gradually turned mature in experimental group; while mucosal epithelial cells arranged loosely in control group. At each time point, new capillaries of experimental group were significantly more than those of control group ( P < 0.05). Scanning electron microscope observation showed the continuous epithelioid cells were observed at anastomosis site with time, and gradually grew into the middle of the patch in experimental group; while less and discontinuous epithelioid cells were observed in control group., Conclusions: Bacterial cellulose patch is feasible to reconstruct cervical tracheal defect in rabbits, and the new mucosa is formed early and completely, so it is expected to be used as artificial trachea material.