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19. Transcriptomic responses to aluminum stress in soybean roots

Author

You, Jiangfeng, Zhang, Hongmei, Liu, Ning, Gao, Lingling, Kong, Lingnan, and Yang, Zhenming

Subjects
Root-crops -- Genetic aspects -- Research, Aluminum compounds -- Research, Soybean -- Genetic aspects -- Research, Genetic transcription -- Research, Soil acidity -- Research, Biological sciences
Abstract

Aluminum (Al) toxicity is the primary limitation to crop production and plant growth in acid soils. Soybean has multiple mechanisms of Al resistance including the complexing and exclusion of Al in root apices by Al-induced citrate secretion. Microarray analysis is available for the identification of genes in soybean. In the present study, Affymetrix soybean genome array was used to identify the Al-induced differentially expressed genes in Al-resistant genotype Jiyu 70. With a cutoff of >2.0-fold (p < 0.05) between non Al-treated and Al-treated root apices, 561 genes were upregulated and 78 genes were downregulated when roots were exposed to 30 µmol/L Al[Cl.sub.3] for 4 h. Quantitative real-time PCR was used to test the microarray data. The analysis showed that nearly half of the Al-responsive genes were of unknown biological function. A higher proportion of genes related to transcription regulation and cell wall processes were observed in Al-induced up- and downregulated genes, respectively. Some genes homologous to the citrate transporter MATE family gene or [C.sub.2][H.sub.2] family transcription factor gene, STOP1, were detected in our analysis. Some genes related to lignin deposition were upregulated, which might be related to Al-induced root elongation inhibition. Key words: aluminium, microarray, gene expression, acid soil. La toxicite de l'aluminium (Al) est la plus importante contrainte a la production vegetale et a la croissance des plantes dans les sols acides. Le soya possede plusieurs mecanismes de resistance a la toxicite de l'Al incluant la complexation et l'exclusion de l'Al dans les apex racinaires par secretion de citrate induite par l'Al. Des puces a ADN sont disponibles pour identifier les genes chez le soya. Dans ce travail, les auteurs ont employe une puce soya Affymetrix pour identifier les genes induits par l'Al chez le genotype resistant a l'Al Jiyu 70. En employant des seuils de 2x pour l'ampleur des changements d' expression (p Mots-cles : aluminium, puce a ADN, expression des genes, sol acide. [Traduit par la Redaction], Introduction Acid soils (pH < 5.5) are the most important limitation to the yield production in many crops, which cover about 30% of the world's arable soils and up to [...]

Published
2011
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26. Severe Acute Respiratory Syndrome Associated Coronavirus Is Detected in Intestinal Tissues of Fatal Cases

Author

Shi, Xueying, Gong, Encong, Gao, Dongxia, Zhang, Bo, Zheng, Jie, Gao, Zifen, Zhong, Yanfeng, Zou, Wanzhong, Wu, Bingquan, Fang, Weigang, Liao, Songlin, Wang, Shenglan, Xie, Zhigang, Lu, Min, Hou, Lin, Zhong, Haohao, Shao, Hongquan, Li, Ning, Liu, Congrong, Pei, Fei, Yang, Jingping, Wang, Yuping, Han, Zhihui, Shi, Xiaohong, Zhang, Qianying, You, Jiangfeng, Zhu, Xiang, and Gu, Jiang

Published
2005

28. Assessing How the Aluminum-Resistance Traits in Wheat and Rye Transfer to Hexaploid and Octoploid Triticale

Author

Ryan, Peter R., Dong, Dengfeng, Teuber, Felix, Wendler, Neele, Mühling, Karl H., Liu, Jie, Xu, Muyun, Moreno, Naike Salvador, You, Jiangfeng, Maurer, Hans-Peter, Horst, Walter J., Delhaize, Emmanuel, Ryan, Peter R., Dong, Dengfeng, Teuber, Felix, Wendler, Neele, Mühling, Karl H., Liu, Jie, Xu, Muyun, Moreno, Naike Salvador, You, Jiangfeng, Maurer, Hans-Peter, Horst, Walter J., and Delhaize, Emmanuel

Abstract

The mechanisms of aluminum (Al) resistance in wheat and rye involve the release of citrate and malate anions from the root apices. Many of the genes controlling these processes have been identified and their responses to Al treatment described in detail. This study investigated how the major Al resistance traits of wheat and rye are transferred to triticale (x Tritosecale Wittmack) which is a hybrid between wheat and rye. We generated octoploid and hexaploid triticale lines and compared them with the parental lines for their relative resistance to Al, organic anion efflux and expression of some of the genes encoding the transporters involved. We report that the strong Al resistance of rye was incompletely transferred to octoploid and hexaploid triticale. The wheat and rye parents contributed to the Al-resistance of octoploid triticale but the phenotypes were not additive. The Al resistance genes of hexaploid wheat, TaALMT1, and TaMATE1B, were more successfully expressed in octoploid triticale than the Al resistance genes in rye tested, ScALMT1 and ScFRDL2. This study demonstrates that an important stress-tolerance trait derived from hexaploid wheat was expressed in octoploid triticale. Since most commercial triticale lines are largely hexaploid types it would be beneficial to develop techniques to generate genetically-stable octoploid triticale material. This would enable other useful traits that are present in hexaploid but not tetraploid wheat, to be transferred to triticale.

Published
2018

31. Assessing How the Aluminum-Resistance Traits in Wheat and Rye Transfer to Hexaploid and Octoploid Triticale

Author

Ryan, Peter R., primary, Dong, Dengfeng, additional, Teuber, Felix, additional, Wendler, Neele, additional, Mühling, Karl H., additional, Liu, Jie, additional, Xu, Muyun, additional, Salvador Moreno, Naike, additional, You, Jiangfeng, additional, Maurer, Hans-Peter, additional, Horst, Walter J., additional, and Delhaize, Emmanuel, additional

Published
2018
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36. Monoclonal Antibodies Against Human Tumor Metastasis Suppressor Gene–1 (TMSG-1): Preparation, Characterization, and Application

Author

Han Zhihui, Pei Fei, Ning Junyu, You Jiangfeng, Yang Shaomin, Cui Xianglin, Wang Yu-ping, Zheng Jie, Wang Jieliang, and Yang Jingpin

Subjects
medicine.drug_class, Blotting, Western, Immunology, Monoclonal antibody, Epitope, Metastasis, Mice, Antigen, Sphingosine N-Acyltransferase, Tumor Cells, Cultured, Genetics, medicine, Animals, Humans, Metastasis suppressor, Hybridomas, biology, Tumor Suppressor Proteins, Antibodies, Monoclonal, Membrane Proteins, medicine.disease, Immunohistochemistry, Molecular biology, Cancer cell, biology.protein, Antibody
Abstract

To better understand the molecular mechanism of metastasis, the monoclonal antibody against tumor metastasis suppressor gene-1 (TMSG-1, one novel gene) was prepared, characterized, and applied in estimating the metastatic potential of human tumors. A dominant epitope, TMSG-1(15)--derived from TMSG-1--was automatically synthesized based on the Fmoc method, and the hapten was conjugated to Imject Maleimide activated mcKLH as a carrier protein. The antigen solution was used to immunize BALB/C mice. Hybridomas were generated and screened by ELISA for specific monoclonal antibodies, and their characterization was performed by Western blotting and immunohistochemical staining. The hybridoma cell line secreting anti-TMSG-1 antibody, designated C8, was established after primary ELISA screening and consequent rapid limited dilution. C8 was IgM in isotyping. The competitive inhibition assay showed that the antibody was TMSG-1 specific. Furthermore, in Western blotting, a protein band of about 45 kD was detected with nonmetastatic variant PC-3M-2B4 and PG-LH7 cells, but not with the isogenetic metastatic variant PC-3M-1E8 and PG-BE1 cells. Immunohistochemistry method showed that positive staining presented in the cytomembrane and cytoplasm of 2B4 and LH7 cells, while 1E8 and BE1 cells were non-reactive. The sections from paraffin-embedded blocks of human cancer (52 cases of breast carcinoma and 41 cases of colon cancer) were stained, showing strongly positive in non-metastatic tumor and weakly positive or negative in metastatic tumor; the strongly positive rate of TMSG-1 expression in human cancers were, respectively, 36%, 7.4%, 52.4%, and 35% in non-metastatic and metastatic breast cancer and non-metastatic and metastatic colon cancer (p = 0.02). The monoclonal antibody developed against synthetic peptide was TMSG-1 specific, and it has promise in Western blot and immunohistochemistry for detecting the TMSG-1 expression of cancer cells and tissues. It may provide an important tool in the study of TMSG-1 expression and function in both experimental and clinical studies. Furthermore, our tests confirmed that TMSG-1 protein has excellent inverse correlation to tumor metastasis potential, with the molecular weight of 45 kD (supporting the encoded protein containing 380 amino acids) and localization in cytomembrane and cytoplasm of tumor cell.

Published
2004

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