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Derris erythrocosta Boonprajan & Sirich., sp. nov., a new species of the genus Derris Lour. (Fabaceae) was discovered from Peninsular, Thailand. The taxon was considered as a distinct species in terms of integrative taxonomy approach. The overall morphology demonstrated that this species most resembled D. pubipetala. According to the macro-morphological and leaves micro-morphological studies, this taxon has several autapomorphies distinctively different from other Derris species, e.g., the presence of reddish midribs of the mature leaflets, sparsely hairy filaments, prominent hairs at base of anthers, and presence of glandular trichomes along the midrib. Additionally, HPLC fingerprints from phytochemical study of this species were also distinct. Results from molecular phylogenetic analyses strongly supported and clearly confirmed its status as a new of the genus Derris.

Work toward completion of the Annonaceae treatment for the Flora of Thailand revealed 18 species previously unrecorded for the country, six of them in the genus Fissistigma. In addition, several species previously placed in synonymy are re-instated, for which we propose three new combinations in the genera Mitrella, Monoon, and Sphaerocoryne.

The Tribe Wisterieae (Zhu 1994), founded on the single genus Wisteria, is emended and recircumscribed based on morphology and data from nuclear ITS and ndhJ-trnF, matK and rbcL chloroplast DNA sequences. This newly enlarged tribe comprises 36 species and 9 infraspecific taxa within 13 described genera. Six genera are new, two are reinstated and five were previously placed in Tribe Millettieae. The genus Adinobotrys is also reinstated comprising two species including the new combination A.vastus. Other reinstated genera include Whitfordiodendron, with four species, and Padbruggea, with three species, including the reinstatement of P.filipes and the new combination P.filipesvar.tomentosa. The existing genera Afgekia, Callerya, Endosamara (with the new combination E.racemosavar.pallida), Sarcodum and Wisteria, with the new combinations W.frutescenssubsp.macrostachya are evaluated. The new genera comprise three Australasian species in Austrocallerya: A.australis, A.megasperma and A.pilipes; Wisteriopsis with five species from east Asia has six new combinations: W.japonica, W.kiangsiensis, W.championii, W.eurybotrya, W.reticulata and W.reticulatavar.stenophylla. Two species comprise the new Thai genus Kanburia: K.tenasserimensis and K.chlorantha. Nanhaia comprises the two species: N.fordii and N.speciosa and the monotypic genera Sigmoidala and Serawaia are based respectively on the species S.kityana and S.strobilifera. Lectotypes are designated for the names Adinobotrysfilipes, A.myrianthus, Millettiabonatiana, Millettiabracteosa, Millettiachampionii, Millettiacinerea, Millettiadielsiana, Millettiakityana, M.maingayi, Millettianitida, Millettiaoocarpa, Millettiapurpurea, M.reticulata, M.reticulatavar.stenophylla, Padbruggeadasyphylla, Pterocarpusaustralis, Robiniaracemosa, Whitfordiodendronscandens, W.sumatranum and Wisteriapallida. A neotype is designated for the name Millettialeiogyna.

Within the legume family, the taxonomic status of subtribe Glycyrrhizinae of tribe Galegeae and of the genus Adinobotrys has been re-assessed. Based on genome skimming data, we conducted phylogenomic analyses of the inverted repeat-lacking clade within subfamily Papilionoideae. The results support the sister relationship between Glycyrrhizeae and Adinobotrys. Glycyrrhizeae is resurrected based on Glycyrrhiza and Glycyrrhizopsis, and a new tribe, Adinobotryeae, is proposed to accommodate Adinobotrys.

&lsquo, Mahat&rsquo, is a well-known medicinal plant utilized in Thailand. The Thai name &lsquo, has been used in many scientific articles for years. However, it is, unpredictably, a homonym of two scientific names in Flora of Thailand, i.e. A. lacucha and A. thailandicus. Additionally, both species are complex due to their high morphological variation. This causes difficulties in species identification especially when this Thai name is referred to as the scientific name for research publication, quality control of pharmaceutical raw materials, and registration of pharmaceutical products. In this study, we scrutinized the taxonomy of &lsquo, by detailed examination of its morphology and distribution, including molecular and qualitative phytochemical studies. Leaf surfaces were inspected using scanning electron microscopy. The phylogeny of both species was studied using DNA sequences of nuclear and plastid regions. Chromatographic fingerprints, focusing on the major active compound oxyresveratrol, were identified using high-performance liquid chromatography. According to our current study, phylogenetic evidence showed that some samples of both species were clustered together in the same clade and phytochemical fingerprints were almost identical. These results are valuable data for taxonomic revision in the near future and reveal the possible utilization of A. thailandicus as a new material source of oxyresveratrol in the pharmaceutical industry.

The inverted repeat-lacking clade (IRLC) is one of the most derived clades within the subfamily Papilionoideae of the legume family, and includes various economically important plants, e.g., chickpeas, peas, liquorice, and the largest genus of angiosperms, Astragalus. Tribe Wisterieae is one of the earliest diverged groups of the IRLC, and its generic delimitation and spatiotemporal diversification needs further clarifications. Based on genome skimming data, we herein reconstruct the phylogenomic framework of the IRLC, and infer the inter-generic relationships and historical biogeography of Wisterieae. We redefine tribe Caraganeae to contain Caragana only, and tribe Astragaleae is reduced to the Erophaca-Astragalean clade. The chloroplast capture scenario was hypothesized as the most plausible explanation of the topological incongruences between the chloroplast CDSs and nuclear ribosomal DNA trees in both the Glycyrrhizinae-Adinobotrys-Wisterieae clade and the Chesneyeae-Caraganeae-Hedysareae clade. A new name, Caragana lidou L. Duan & Z.Y. Chang, is proposed within Caraganeae. Thirteen genera are herein supported within Wisterieae, including a new genus, Villosocallerya L. Duan, J. Compton & Schrire, segregated from Callerya. Our biogeographic analyses suggest that Wisterieae originated in the late Eocene and its most recent common ancestor (MRCA) was distributed in continental southeastern Asia. Lineages of Wisterieae remained in the ancestral area from the early Oligocene to the early Miocene. By the middle Miocene, Whitfordiodendron and the MRCA of Callerya-Kanburia-Villosocallerya Clade became disjunct between the Sunda area and continental southeastern Asia, respectively; the MRCA of Wisteria migrated to North America via the Bering land bridge. The ancestor of Austrocallerya and Padbruggea migrated to the Wallacea-Oceania area, which split in the early Pliocene. In the Pleistocene, Wisteria brachybotrys, W. floribunda and Wisteriopsis japonica reached Japan, and Callerya cinerea dispersed to South Asia. This study provides a solid phylogenomic for further evolutionary/biogeographic/systematic investigations on the ecologically diverse and economically important IRLC legumes.

The Tribe Wisterieae (Zhu 1994), founded on the single genus Wisteria, is emended and recircumscribed based on morphology and data from nuclear ITS and ndhJ-trnF, matK and rbcL chloroplast DNA sequences. This newly enlarged tribe comprises 36 species and 9 infraspecific taxa within 13 described genera. Six genera are new, two are reinstated and five were previously placed in Tribe Millettieae. The genus Adinobotrys is also reinstated comprising two species including the new combination A.vastus. Other reinstated genera include Whitfordiodendron, with four species, and Padbruggea, with three species, including the reinstatement of P.filipes and the new combination P.filipesvar.tomentosa. The existing genera Afgekia, Callerya, Endosamara (with the new combination E.racemosavar.pallida), Sarcodum and Wisteria, with the new combinations W.frutescenssubsp.macrostachya are evaluated. The new genera comprise three Australasian species in Austrocallerya: A.australis, A.megasperma and A.pilipes; Wisteriopsis with five species from east Asia has six new combinations: W.japonica, W.kiangsiensis, W.championii, W.eurybotrya, W.reticulata and W.reticulatavar.stenophylla. Two species comprise the new Thai genus Kanburia: K.tenasserimensis and K.chlorantha. Nanhaia comprises the two species: N.fordii and N.speciosa and the monotypic genera Sigmoidala and Serawaia are based respectively on the species S.kityana and S.strobilifera. Lectotypes are designated for the names Adinobotrysfilipes, A.myrianthus, Millettiabonatiana, Millettiabracteosa, Millettiachampionii, Millettiacinerea, Millettiadielsiana, Millettiakityana, M.maingayi, Millettianitida, Millettiaoocarpa, Millettiapurpurea, M.reticulata, M.reticulatavar.stenophylla, Padbruggeadasyphylla, Pterocarpusaustralis, Robiniaracemosa, Whitfordiodendronscandens, W.sumatranum and Wisteriapallida. A neotype is designated for the name Millettialeiogyna.

Derris solorioides is described as a new species and illustrated. This species is only the second calciphilous and true-paniculate species of Derris ever recorded. The species was found in isolated and protected limestone areas surrounded by agricultural areas in Nakhon Sawan province, North-Central Thailand. It is characterized by its rather smaller flowers but with more ovules than other species of Derris, and 1-winged pods showing a dark-coloured pericarp around the seeds without thickening of the pericarp. The characters of the pods are similar to those found in Solori, a genus once synonymized with Derris and, therefore, the epithet ‘solorioides’ was assigned. This species appeared to be a distinct taxon in the molecular phylogeny, separate from its morphologically highly similar species, D. marginata. It is also a member of a lineage of Derris consisting of species with a deviating type of inflorescence: intermediate forms and true panicles, which is quite uncommon in this genus. The relationship with its closely related species is discussed, and a key to the species of Derris in the ‘deviating type of inflorescence’ clade is presented.

Aim The historical biogeography of three Palaeotropical legume genera, Aganope, Brachypterum and Derris, was investigated with the aim of (1) evaluating competing hypotheses on the origins of Palaeotropical intercontinental disjunctions (PIDs), and (2) inferring spatio-temporal diversification patterns in tropical Southeast Asia. Location Palaeotropics. Methods Plastid (trnL–F IGS, psbA–trnH IGS and trnK–matK ORF) and nuclear ribosomal (ITS/5.8S) DNA sequence data, covering the geographical distribution of all three genera, were analysed using an uncorrelated-rates relaxed molecular clock model. Ancestral areas were reconstructed using a likelihood approach implementing the dispersal–extinction–cladogenesis model (Lagrange) and a Bayesian approach to dispersal–vicariance analysis (S-DIVA). Results A wide ancestral distribution in Africa and Asia was inferred for the Aganope stem and crown groups, with a vicariance event between Africa and Asia in the early Miocene. The Southeast Asian mainland was inferred as the ancestral area for both the Brachypterum and the Derris crown groups. The reconstructions indicated numerous dispersal events westwards to India, and eastwards across Wallace's Line to New Guinea from the middle Miocene onwards. Two dispersal events from Asia to Africa, in the Miocene–Pliocene in Brachypterum and in the Pliocene–Pleistocene in Derris, were deduced. Main conclusions The PID in Aganope is likely to be the result of vicariance, caused by climatic deterioration subsequent to the Middle Miocene Climatic Optimum. The inferred PIDs in Brachypterum and Derris in the middle Miocene to Pliocene–Pleistocene are consistent with long-distance dispersal. The biogeographical patterns of Brachypterum and Derris are similar to patterns identified in other Southeast Asian plant taxa, and highly congruent with geological events in Southeast Asia facilitating dispersal from the early Miocene onwards. Preadaptation to several environmental conditions and habitats including mangrove swamps, and high dispersal capabilities by hydrochory may explain the wide distributions of some species and frequent dispersal across oceanic water bodies separating western and eastern Malesia.

PREMISE OF THE STUDY Palaeotropic Derris-like taxa (family Fabaceae, tribe Millettieae) comprise 6-9 genera. They are well known as important sources of rotenone toxin, which are used as organic insecticide and fish poison. However, their phylogenetic relationships and classification are still problematic due to insufficient sampling and high morphological variability. METHODS Fifty species of palaeotropic Derris-like taxa were sampled, which is more than in former studies. Three chloroplast genes (trnK-matK, trnL-F IGS, and psbA-trnH IGS) and nuclear ribosomal ITS /5.8S were analyzed using parsimony and Bayesian methods. KEY RESULTS Parsimony and Bayesian analyses of individual and combined markers show more or less similar tree topologies (only varying in terminal branches). The old-world monophyletic genera Aganope, Brachypterum, and Leptoderris are distinct from Derris s.s., and their generic status is here confirmed. Aganope may be classified into two or three subgeneric taxa. Paraderris has to be included in Derris s.s. to form a monophyletic group. The genera Philenoptera, Deguelia, and Lonchocarpus are monophyletic and distinct from each other and clearly separate from Derris s.s. Morphologically highly similar species of Derris s.s. are shown to be unrelated. Our study shows that previous infrageneric classifications of Derris are incorrect. Paraderris elliptica may contain several cryptic lineages that need further investigation. CONCLUSIONS The concept of the genus Derris s.s. should be reorganized with a new generic circumscription by including Paraderris but excluding Brachypterum. Synapomorphic morphological features will be examined in future studies, and the status of the newly defined Derris and its closely related taxa will be formalized.

Callerya chlorantha and C. tenasserimensis, here illustrated and described as new to science, are found in dry deciduous or bamboo forest in Kanchanaburi and Ratchaburi provinces in Thailand. Callerya chlorantha, characterized by having pale green flowers that are rare in the genus, grows on limestone. This species is considered as Data Deficient (DD) at this time because there are not yet sufficient data about its distribution, abundance or threats. Callerya tenasserimensis has glabrous leaves and stems and rather small, purple or maroon flowers with golden-brown hairs on the calyx and abaxial side of the standard petal. The conservation status of this species is assessed as Vulnerable (VU).

In this revision of the Thai species of the genus Derris and the morphologically similar genera, Aganope and Paraderris, all currently recognized species are enumerated. Two new species, Derris glabra Sirichamorn and Derris pseudomarginata Sirichamorn, are described and illustrated. Keys to the genera and species are provided, together with descriptions and notes for all taxa.