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14. Sterilized, freeze-dried amniotic membrane : a useful substrate for ocular surface reconstruction.

Author

Nakamura, Takahiro, Yoshitani, Makoto, Rigby, Helen, Fullwood, Nigel J., Ito, Wakana, Inatomi, Tsutomu, Sotozono, Chie, Nakamura, Tatsuo, Shimizu, Yasuhiko, Kinoshita, Shigeru, Nakamura, Takahiro, Yoshitani, Makoto, Rigby, Helen, Fullwood, Nigel J., Ito, Wakana, Inatomi, Tsutomu, Sotozono, Chie, Nakamura, Tatsuo, Shimizu, Yasuhiko, and Kinoshita, Shigeru

Abstract

PURPOSE. To examine the feasibility of using sterilized, freeze-dried amniotic membrane (FD-AM) as a substrate for cultivating autologous corneal epithelial cells for ocular surface reconstruction. METHODS. Human AM deprived of amniotic epithelial cells by incubation with EDTA was freeze dried, vacuum packed, and sterilized with -irradiation. The resultant FD-AM was characterized for its physical, biological, and morphologic properties by stretch stress tests, immunohistochemistry, electron microscopy, and cell culture. In addition, 3 weeks after an ocular surface injury, the conjunctivalized corneal surfaces of eyes in eight rabbits were surgically reconstructed by transplantation of autologous cultivated corneal epithelial cells on FD-AM. RESULTS. A stretch stress test revealed no significant differences between sterilized FD-AM and cryopreserved AM. Immunohistochemistry for several extracellular matrix molecules and electron microscopic analysis of FD-AM revealed that the process of drying and irradiation did not affect its biological and morphologic properties. The corneal epithelial cells cultivated on FD-AM had four to five stratified, well-differentiated cell layers. Corneas that were grafted with the cultivated corneal epithelial cells on FD-AM were clear and were all epithelialized at 10 days after surgery. CONCLUSIONS. The sterilized, freeze-dried AM retained most of the physical, biological, and morphologic characteristics of cryopreserved AM; consequently, it is a useful biomaterial for ocular surface reconstruction.

Published
2004

17. IN SITU TISSUE ENGINEERING OF THE TRACHEA

Author

Nakamura, Tatsuo, primary, Kawanami, Eriko, additional, Fukuda, Seijunn, additional, Ueda, Hiroki, additional, Kimura, Dai, additional, Kaino, Kenji, additional, Kobayashi, Eizaburou, additional, Yoshitani, Makoto, additional, Toba, Toshinari, additional, Shimizu, Yasuhiko, additional, Kanemaru, Shinnichi, additional, Itoh, Tadao, additional, Nakahara, Taka, additional, Hori, Yoshito, additional, and Itoi, Shinnichi, additional

Published
2002
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23. NOVEL TRACHEAL PROSTHESIS AND IN SITU TISSUE ENGINEERING TO ENHANCE TISSUE REGENERATION ON IT

Author

Nakamura, Tatsuo, primary, Kawanami, Eriko, additional, Ueda, Hiroki, additional, Fukuda, Seijun, additional, Itoi, Shin-ichi, additional, Shigeno, Keiji, additional, Inoue, Masatoshi, additional, Yoshitani, Makoto, additional, Yu, Liu, additional, Toba, Toshinari, additional, Hori, Yoshio, additional, Noguchi, Tetsuya, additional, Lynn, Andrew K, additional, Kanemaru, Shin-ich, additional, Kojima, Hisayoshi, additional, and Shimizu, Yasuhiko, additional

Published
2001
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27. Regeneration of canine peroneal nerve with the use of a polyglycolic acid–collagen tube filled with laminin‐soaked collagen sponge: a comparative study of collagen sponge and collagen fibers as filling materials for nerve conduits

Author

Toba, Toshinari, primary, Nakamura, Tatsuo, additional, Shimizu, Yasuhiko, additional, Matsumoto, Kazuya, additional, Ohnishi, Katsunori, additional, Fukuda, Seijun, additional, Yoshitani, Makoto, additional, Ueda, Hiroki, additional, Hori, Yoshio, additional, and Endo, Katsuaki, additional

Published
2001
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28. Sterilized, freeze-dried amniotic membrane: a useful substrate for ocular surface reconstruction.

Author

Nakamura T, Yoshitani M, Rigby H, Fullwood NJ, Ito W, Inatomi T, Sotozono C, Nakamura T, Shimizu Y, and Kinoshita S

Subjects
Animals, Cell Culture Techniques methods, Coculture Techniques, Extracellular Matrix Proteins metabolism, Feasibility Studies, Freeze Drying, Humans, Immunohistochemistry, Microscopy, Electron, Scanning, Rabbits, Plastic Surgery Procedures, Sterilization, Amnion ultrastructure, Cell Transplantation methods, Corneal Diseases surgery, Epithelial Cells transplantation, Epithelium, Corneal cytology
Abstract

Purpose: To examine the feasibility of using sterilized, freeze-dried amniotic membrane (FD-AM) as a substrate for cultivating autologous corneal epithelial cells for ocular surface reconstruction., Methods: Human AM deprived of amniotic epithelial cells by incubation with EDTA was freeze dried, vacuum packed, and sterilized with gamma-irradiation. The resultant FD-AM was characterized for its physical, biological, and morphologic properties by stretch stress tests, immunohistochemistry, electron microscopy, and cell culture. In addition, 3 weeks after an ocular surface injury, the conjunctivalized corneal surfaces of eyes in eight rabbits were surgically reconstructed by transplantation of autologous cultivated corneal epithelial cells on FD-AM., Results: A stretch stress test revealed no significant differences between sterilized FD-AM and cryopreserved AM. Immunohistochemistry for several extracellular matrix molecules and electron microscopic analysis of FD-AM revealed that the process of drying and irradiation did not affect its biological and morphologic properties. The corneal epithelial cells cultivated on FD-AM had four to five stratified, well-differentiated cell layers. Corneas that were grafted with the cultivated corneal epithelial cells on FD-AM were clear and were all epithelialized at 10 days after surgery., Conclusions: The sterilized, freeze-dried AM retained most of the physical, biological, and morphologic characteristics of cryopreserved AM; consequently, it is a useful biomaterial for ocular surface reconstruction.

Published
2004
Full Text
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