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1. Subcritical Water-Carbon Dioxide Pretreatment of Oil Palm Mesocarp Fiber for Xylooligosaccharide and Glucose Production

Author

Norlailiza Ahmad, Mohd Rafein Zakaria, Mohd Zulkhairi Mohd Yusoff, Shinji Fujimoto, Hiroyuki Inoue, Hidayah Ariffin, Mohd Ali Hassan, and Yoshihoto Shirai

Subjects
oil palm mesocarp fiber, subcritical H2O-CO2, pretreatment, xylooligosaccharides, glucose, Organic chemistry, QD241-441
Abstract

The present work aimed to investigate the pretreatment of oil palm mesocarp fiber (OPMF) in subcritical H2O-CO2 at a temperature range from 150–200 °C and 20–180 min with CO2 pressure from 3–5 MPa. The pretreated solids and liquids from this process were separated by filtration and characterized. Xylooligosaccharides (XOs), sugar monomers, acids, furans and phenols in the pretreated liquids were analyzed by using HPLC. XOs with a degree of polymerization X2–X4 comprising xylobiose, xylotriose, xylotetraose were analyzed by using HPAEC-PAD. Enzymatic hydrolysis was performed on cellulose-rich pretreated solids to observe xylose and glucose production. An optimal condition for XOs production was achieved at 180 °C, 60 min, 3 MPa and the highest XOs obtained was 81.60 mg/g which corresponded to 36.59% of XOs yield from total xylan of OPMF. The highest xylose and glucose yields obtained from pretreated solids were 29.96% and 84.65%, respectively at cellulase loading of 10 FPU/g-substrate.

Published
2018
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2. Subcritical Water-Carbon Dioxide Pretreatment of Oil Palm Mesocarp Fiber for Xylooligosaccharide and Glucose Production

Author

Mohd Ali Hassan, Yoshihoto Shirai, Norlailiza Ahmad, Mohd Zulkhairi Mohd Yusoff, Hidayah Ariffin, Shinji Fujimoto, Mohd Rafein Zakaria, and Hiroyuki Inoue

Subjects
0106 biological sciences, Chemical Phenomena, 020209 energy, Phytochemicals, Oligosaccharides, Pharmaceutical Science, Glucuronates, 02 engineering and technology, Cellulase, Arecaceae, Xylose, 01 natural sciences, subcritical H2O-CO2, Article, Analytical Chemistry, oil palm mesocarp fiber, lcsh:QD241-441, chemistry.chemical_compound, lcsh:Organic chemistry, 010608 biotechnology, Enzymatic hydrolysis, Drug Discovery, 0202 electrical engineering, electronic engineering, information engineering, Xylobiose, Fiber, Physical and Theoretical Chemistry, glucose, Cellulose, Sugar, Chromatography, biology, Hydrolysis, Organic Chemistry, Water, Carbon Dioxide, Hydrogen-Ion Concentration, pretreatment, Xylan, chemistry, Chemistry (miscellaneous), biology.protein, xylooligosaccharides, Molecular Medicine, Sugars, Xylooligosaccharide
Abstract

The present work aimed to investigate the pretreatment of oil palm mesocarp fiber (OPMF) in subcritical H2O-CO2 at a temperature range from 150&ndash, 200 &deg, C and 20&ndash, 180 min with CO2 pressure from 3&ndash, 5 MPa. The pretreated solids and liquids from this process were separated by filtration and characterized. Xylooligosaccharides (XOs), sugar monomers, acids, furans and phenols in the pretreated liquids were analyzed by using HPLC. XOs with a degree of polymerization X2&ndash, X4 comprising xylobiose, xylotriose, xylotetraose were analyzed by using HPAEC-PAD. Enzymatic hydrolysis was performed on cellulose-rich pretreated solids to observe xylose and glucose production. An optimal condition for XOs production was achieved at 180 &deg, C, 60 min, 3 MPa and the highest XOs obtained was 81.60 mg/g which corresponded to 36.59% of XOs yield from total xylan of OPMF. The highest xylose and glucose yields obtained from pretreated solids were 29.96% and 84.65%, respectively at cellulase loading of 10 FPU/g-substrate.

Published
2018

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