69 results on '"Yoshifumi Iwamoto"'
Search Results
2. A parallel object-oriented total architecture: A-NET.
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Takanobu Baba, Tsutomu Yoshinaga, Tohru Iijima, Yoshifumi Iwamoto, Masahiro Hamada, and Mitsuru Suzuki
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- 1990
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Catalog
3. Electrical Network of Single-Crystalline Metal Oxide Nanoclusters Wired by π-Molecules
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Tomoyuki Akutagawa, Katsuya Ishiguro, Ryo Tsunashima, Chisato Kato, Yusuke Baba, Sadafumi Nishihara, Katsuya Ichihashi, Yu-Fei Song, Katsuya Inoue, and Yoshifumi Iwamoto
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Materials science ,Inorganic chemistry ,Cationic polymerization ,General Chemistry ,General Medicine ,Catalysis ,Nanoclusters ,chemistry.chemical_compound ,Delocalized electron ,Crystallography ,chemistry ,Polyoxometalate ,Molecule ,Pyridinium ,Single crystal ,Tetrathiafulvalene - Abstract
In a mixed-valence polyoxometalate, electrons are usually delocalized within the cluster anion because of low level of inter-cluster interaction. Herein, we report the structure and electrical properties of a single crystal in which mixed-valence polyoxometalates were electrically wired by cationic π-molecules of tetrathiafulvalene substituted with pyridinium. Electron-transport characteristics are suggested to represent electron hopping through strong interactions between cluster and cationic π-molecules. more...
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- 2014
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4. Expression of Syndecan-2, -4, and Fibroblast Growth Factor Receptor Type 1 in Human Periodontal Ligament Fibroblasts and Down-regulation of These Membrane Proteins during Maturation in Culture
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Masayuki Morishita, Mitsuhide Noshiro, Yoshifumi Iwamoto, M.A.H Bachchu, Atsushi Shimazu, and Yukio Kato
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0301 basic medicine ,Periodontal Ligament ,Basic fibroblast growth factor ,Down-Regulation ,Biology ,Syndecan 1 ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,stomatognathic system ,Humans ,Regeneration ,Periodontal fiber ,RNA, Messenger ,Receptor, Fibroblast Growth Factor, Type 1 ,General Dentistry ,Cells, Cultured ,Membrane Glycoproteins ,Reverse Transcriptase Polymerase Chain Reaction ,Fibroblast growth factor receptor 2 ,Fibroblast growth factor receptor 1 ,Receptor Protein-Tyrosine Kinases ,Cell Differentiation ,030206 dentistry ,Fibroblast growth factor receptor 4 ,Fibroblasts ,Fibroblast growth factor receptor 3 ,Alkaline Phosphatase ,Blotting, Northern ,Receptors, Fibroblast Growth Factor ,Molecular biology ,carbohydrates (lipids) ,030104 developmental biology ,chemistry ,Fibroblast growth factor receptor ,Fibroblast Growth Factor 2 ,Proteoglycans ,Syndecan-4 ,Syndecan-2 ,Cell Division - Abstract
Syndecans are transmembrane heparan sulfate proteoglycans. They are known to interact with basic fibroblast growth factor (bFGF), and it has been suggested that they play important roles in the growth, morphology, and migration of a variety of cell types. We examined the expression of syndecans and fibroblast growth factor receptor type 1 (FGFR1) in periodontal ligament (PDL) cells, because these membrane proteins may play roles in the control of growth and differentiation during regeneration of PDL. Reverse-transcription/polymerase chain-reaction (RT-PCR) showed that PDL cells expressed syndecan-2 and -4 mRNAs. This was confirmed by sequence analysis of the PCR products. When PDL cells were maintained for 25 days, alkaline phosphatase (ALPase) activity gradually increased and reached a maximal level on day 20. Northern blotting analysis showed that PDL cells expressed 2.3-kb syndecan-2, 2.6-kb syndecan-4, and 2.8-kb FGFR1 mRNAs throughout the entire culture period, whereas no syndecan-1 mRNA was detectable by this method. Maximal levels of syndecan-2, -4, and FGFR1 mRNAs were observed on day 5. However, their levels were markedly decreased on days 20 and 25. Accordingly, the inhibitory effect of bFGF on ALPase activity was less on day 20 than on day 5. When PDL cells were pre-treated with heparitinase, a mitogenic response of PDL cells to bFGF was decreased. These observations indicate that PDL cells express syndecan-2, -4, and FGFR1 mRNAs, and that those levels are changed with the increase in ALPase activity in culture. The reductions in syndecan-2, -4, and FGFR1 levels may be involved in the control of growth and differentiation of PDL cells during development and regeneration. more...
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- 1999
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5. An Analytical Study on Gender Differences in Self‐Reported Oral Health Care and Problems of Japanese Employees
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Hisako Sasahara, Makoto Kawamura, F. A. C. Wright, Sookja Suh, Yoshifumi Iwamoto, and Yukiko Yamasaki
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Gerontology ,medicine.medical_specialty ,business.industry ,Public Health, Environmental and Occupational Health ,Psychological intervention ,Alternative medicine ,Preventive Dentistry ,Occupational safety and health ,Test (assessment) ,Tooth mobility ,Dental floss ,Medicine ,Oral health care ,business ,Demography - Abstract
An Analytical Study on Gender Differences in Self-Reported Oral Health Care and Problems of Japanese Employees: Makoto KAWAMURA, et al. Department of Preventive Dentistry, Hiroshima University School of Dentistry—The purpose of this survey was to clarify gender differences in self-reported oral health care and problems of Japanese employees. A 60-item questionnaire named Dental Checker® was used in a project on oral health in the working place. Subjects were 77,845 Japanese employees (males, 52,345; females, 25,500). The Mantel-Haenszel chi-square test was used to clarify gender differences among respondents. Of great significance was a finding that 81.8% of females felt uncomfortable when they slept without brushing their teeth, as contrast with 58.8% of males (P more...
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- 1999
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6. Comparison of health behaviour and oral/medical conditions in non-insulin-dependent (type II) diabetics and non-diabetics
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Setsuko Fukuda, Kunio Kawabata, Makoto Kawamura, and Yoshifumi Iwamoto
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Adult ,Male ,medicine.medical_specialty ,Health Status ,Health Behavior ,Dental Plaque ,Dentistry ,Oral Health ,Disease ,Severe periodontitis ,Tooth mobility ,Tooth Loss ,Gingivitis ,Diabetes mellitus ,Internal medicine ,Weight management ,medicine ,Humans ,Periodontal Pocket ,Dental Calculus ,Periodontitis ,General Dentistry ,Fatigue ,Aged ,business.industry ,Calculus (dental) ,Feeding Behavior ,Middle Aged ,Oral Hygiene ,medicine.disease ,Diabetes Mellitus, Type 2 ,Case-Control Studies ,Female ,Periodontal Index ,Tooth Mobility ,medicine.symptom ,Energy Intake ,business - Abstract
One hundred and two dentate patients with type II diabetes mellitus and 98 non-diabetic subjects were examined for oral conditions and metabolic state. Self-reported health behaviour was analysed. From factor analysis four factors emerged: general health behaviour (GHB), perceived fatigue (PF), diet control (DC) and regular diet (RD). In diabetics PF, DC and RD were significantly higher than that in non-diabetics. Patients with diabetes were more likely to control their disease through a programme of decreased kilojoule intake leading to weight management. However, they tended to tire. The mean gingivitis index was significantly higher (p more...
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- 1998
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7. The effects of oestrogen on osteocalcin production by human periodontal ligament cells
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Yoshifumi Iwamoto, Atsushi Shimazu, T Yamamura, M.A.H Bachchu, and Masayuki Morishita
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Adult ,Male ,endocrine system ,medicine.medical_specialty ,Time Factors ,Periodontal Ligament ,Osteocalcin ,Cell Culture Techniques ,Immunoenzyme Techniques ,stomatognathic system ,Internal medicine ,polycyclic compounds ,medicine ,Humans ,Sandwich enzyme immunoassay ,Periodontal fiber ,skin and connective tissue diseases ,General Dentistry ,Cells, Cultured ,Dose-Response Relationship, Drug ,Estradiol ,biology ,Chemistry ,Cell Biology ,General Medicine ,In vitro ,Culture Media ,Endocrinology ,Otorhinolaryngology ,biology.protein ,Female ,hormones, hormone substitutes, and hormone antagonists - Abstract
The purpose was to investigate the effects of oestradiol on the function of periodontal ligament (PDL) cells by measuring the production of osteocalcin in vitro. Cells were obtained from the healthy periodontal ligament of teeth extracted from two males and two females for orthodontic reasons. Serum-free medium was used when testing the effects of oestradiol on PDL cells. The amount of osteocalcin in the culture medium was analysed by two-step sandwich enzyme immunoassay in the presence or absence of oestradiol. It was shown that oestradiol enhanced the production of osteocalcin by PDL cells in a time- and dose-dependent manner. PDL cells obtained from both male and female donors were affected by oestradiol. It thus appears that oestradiol is one of the factors important for PDL cells to express their function. more...
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- 1998
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8. Comparison of the Effectiveness of Electric versus Manual Toothbrushes on Clinical Condition in Initial Treatment and Maintenance Therapy
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Yoshifumi Iwamoto, Hisako Sasahara, Kunio Kawabata, Masayuki Morishita, Fumie Nakata, and Makoto Kawamura
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Maintenance therapy ,business.industry ,Anesthesia ,Medicine ,Initial treatment ,business - Abstract
電動歯ブラシは, プラーク除去や歯肉炎の改善に対して手用歯ブラシと同等の効果があることが報告されている。しかし電動歯ブラシを4週間から8週間と, 短期間使用した時の効果についての報告は多いが, 1年以上の長期間にわたって使用した場合の効果についての報告は少ない。そこで今回は, 初期治療およびメインテナンスの期間中も電動歯ブラシを使用した場合の臨床的パラメーターに対する効果について検討する目的で研究を行った。初期治療において電動歯ブラシ (インタープラーク®,IP) によるブラッシング指導を受け, 引き続きメインテナンスの期間中もIPを使用している患者23名をIP群とした。同様に初期治療およびメインテナンスを受けている患者で, 手用歯ブラシを使用している患者 (14名) を手用歯ブラシ群とし, 両群について初期治療終了後, 3ヵ月毎にリコールを行い, 6カ月, 12カ月および24カ月後にPCR, PD, およびBOPなどの臨床診査を行った。その結果, IP群, 手用歯ブラシ群ともに初期治療によりPCR, PDおよびBOPなどの臨床的パラメーターは改善され, メインテナンスの期間中も良好に推移した。以上の結果より, IPは初期治療およびメインテナンスを継続している患者が長期間使用した場合でも, 手用歯ブラシと同等の効果があることが示された。 more...
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- 1997
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9. Comparison of the Effectiveness of Toothbrushing Instruction Using Electric Versus Manual Toothbrushes
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Kunio Kawabata, Masayuki Morishita, Hisako Sasahara, Yoshifumi Iwamoto, Makoto Kawamura, and Fumie Nakata
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business.industry ,Medicine ,business - Published
- 1996
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10. Immunohistochemical Study of γδ T Cells in Human Gingival Tissues
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Masami Fukunaga, Masayuki Morishita, Takashi Takata, Kazuko Kawahara, Makoto Kawamura, and Yoshifumi Iwamoto
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Pathology ,medicine.medical_specialty ,medicine.diagnostic_test ,Chemistry ,Gingival tissue ,Connective tissue ,medicine.disease_cause ,Inflammatory cell infiltration ,Gingival epithelium ,medicine.anatomical_structure ,Biopsy ,medicine ,Periodontics ,Immunohistochemistry ,Irritation - Abstract
The distribution and the density of γδ T cells in human gingival tissues were examined immunohistochemically in biopsy samples obtained from 20 subjects. Few γδ T cells were observed in gingival tissue free from inflammatory cell infiltration, but were found, albeit in low numbers, in association with inflammatory cell infiltration, especially T cells. This relationship with T cells was confirmed statistically. The ratios of 78 T cells to T cells in the epithelia and in the connective tissue were calculated in the sections in which more than 500 CD3-positive cells were identified. Seven of eight such epithelial specimens showed a ratio of less than 1% and one less than 2% (mean ± SD; 0.8% ± 0.4). In the connective tissue, 8 of 13 such specimens showed less than 1%, three less than 2%, one 3%, and one 7% (1.4% ± 1.9). These results suggest that basically γδ T cells are not resident cells in the gingival epithelium such as comprise the first defense line against exogenous irritation. They may play some role... more...
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- 1995
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11. A Study on the Expression of Heat Shock Protein Genes in the Human Periodontal Ligament Fibroblasts
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Masami Fukunaga, Yoshifumi Iwamoto, Masayuki Morishita, Kiyoko Tsujimura, Kazuko Kawahara, and Keiko Tsuruda
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Periodontitis ,Chemistry ,business.industry ,Heat shock protein ,medicine ,Dentistry ,Periodontal fiber ,medicine.disease ,business ,Molecular biology ,Gene - Abstract
熱ショック蛋白質 (hsp) と歯周炎との関わりを探る第一歩として, ヒト歯根膜由来線維芽細胞PDL) におけるhsp遺伝子発現をRT-PCR法を用いて検討した。 (Hsp70, hsp60遺伝子ともに無刺激下でもPDLにおける発現が確認された。また, hsp70は, 42℃, 1時間のheatshock処理およびActinoobacillus actinomycetemcomitans, E. coliの破砕液の24時間刺激により, その発現の増強が認められたが, Streptococcus sanguis, Lactobacillus caseiの破砕液刺激では変化は認められなかった。また, hsp60については上記の刺激によっても発現の増強は認められなかった。今回の結果から, 細菌破砕液がhsp70発現を誘導し得るストレスとなり, また歯周病原性細菌による感染の際に歯周組織のhsp70の発現量が増大する可能性が推察された。本論文の要旨は, 第37回秋季日本歯周病学会 (1994年10月6日) において発表した。 more...
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- 1995
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12. Effects of Sex Hormones on Production of Prostaglandin E2by Human Peripheral Monocytes
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Masayuki Morishita, Yoshifumi Iwamoto, and Masaharu Miyagi
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Adult ,Male ,medicine.medical_specialty ,Lipopolysaccharide ,Prostaglandin ,Biology ,Monocytes ,chemistry.chemical_compound ,Internal medicine ,medicine ,Humans ,Testosterone ,Prostaglandin E2 ,Incubation ,Progesterone ,Analysis of Variance ,Estradiol ,Prostaglandins E ,Radioimmunoassay ,Gingivitis ,In vitro ,Endocrinology ,chemistry ,Periodontics ,Female ,lipids (amino acids, peptides, and proteins) ,Hormone ,medicine.drug - Abstract
The effects of sex hormones on the vitro production of prostaglandin (PG) E2 by monocytes were investigated. Monocytes were obtained from heparinized peripheral blood of healthy adults and incubated for 24 hours with lipopolysaccharide (LPS) and sex hormones. After incubation, the medium was assayed for PGE2 by means of radioimmunoassay. PGE2 production by monocytes was enhanced by progesterone. Estradiol reduced PGE2 production at 0.4 ng/ml, but enhanced it at 20 ng/ml. Testosterone reduced PGE2 production. The reduced PGE2 production by monocytes treated with 0.4 ng/ml of estradiol was restored to the control level by addition of progesterone at 20 ng/ml. These results suggest that sex hormones may modulate gingival inflammation mediated by PGE2. more...
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- 1993
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13. Relationship between CPITN and oral health behaviour in Japanese adults
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Makoto Kawamura, Yoshifumi Iwamoto, Hisako Sasahara, Kunio Kawabata, Fac Wright, and K. Konishi
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Adult ,Male ,Toothbrushing ,Oral Hygiene Index ,Health Behavior ,Bleeding on probing ,Dentistry ,Oral health ,Oral hygiene ,Sex Factors ,Japan ,Sex factors ,Surveys and Questionnaires ,Humans ,Medicine ,Dental Calculus ,General Dentistry ,Periodontal Diseases ,business.industry ,Dental health ,Age Factors ,Health behaviour ,Middle Aged ,Oral Hygiene ,Regression Analysis ,Positive relationship ,Female ,Periodontal Index ,medicine.symptom ,business - Abstract
Many studies on toothbrushing have concentrated on clinically diagnosing plaque and measuring periodontal status as indicators of oral health behaviour. From a behavioural point of view, however, the more important objective is the health behaviour itself. To investigate the relationship of oral health behaviour to periodontal status, 517 urban employees in Japan (249 men and 268 women aged 20-59 years) responded to a 20-item dental health behaviour questionnaire, entitled the HU-DBI, and had their periodontal conditions examined using the CPITN. Only 1 per cent were found with a healthy periodontium (Code 0), and 9 per cent had bleeding on probing (Code 1). Calculus (Code 2) was the most prevalent condition with 51 per cent of subjects having this code as the worst condition, followed by shallow pockets (Code 3) in 30 per cent, and deep pockets (Code 4) in 9 per cent of the sample. The mean HU-DBI score was 4.2 (out of 12). Females had somewhat higher scores than males (4.4 vs. 4.0, p < 0.05). CPITN had a negative relationship with the HU-DBI (r = -0.26, p < 0.001), and a positive relationship with age (r = 0.40, p < 0.001). Similar relationships were observed in each gender. These data demonstrate the relationship of age with periodontal status, and periodontal status with oral health behaviour. A two-dimensional matrix of HU-DBI score by CPITN may provide a simple and effective means of identifying low and high risk individuals. more...
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- 1993
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14. Maternal and Child Dental Health Care at a Public Health Center. II. Relationship between Caries Increment and Life Style in 1.5-year-old Children with and without Caries
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Hisako Sasahara, Yoshifumi Iwamoto, Masaharu Miyagi, Kunio Kawabata, Masayuki Morishita, and Makoto Kawamura
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medicine.medical_specialty ,Pediatrics ,Multivariate analysis ,business.industry ,Life style ,Dental health ,Public health ,Caries prevalence ,Tooth brushing ,medicine ,Screening method ,business ,Breast feeding ,Demography - Abstract
The purpose of this study was to extract the factors which affect the caries increment between 1.5 and 3 years of age by dividing the subjects into two groups, one with and the other without caries at 1.5 years of age.The subjects were 1641 children who received a 3-year-old dental health examination at a public health center in Hiroshima City between April 1990 and November 1991.Eight factors associated with caries prevalence at 3 years of age were examined using multivariate analysis. These factors were ‘Family type’, ‘Order of birth’, ‘Interruption of breast feeding’, ‘Bottle feeding at 1.5 years of age’, ‘Regularity of between-meal snacks’, ‘Drinking sugary beverages’, ‘Watching TV at meals’ and ‘Tooth brushing with mother's help’.The results were as follows.1) The factors related to caries increment from 1.5 to 3 years of age for the children without caries at 1.5 years of age were ‘Drinking sugary beverages’, ‘Interruption of breast feeding’, ‘Watching TV at meals’ and ‘Bottle feeding at 1.5 years of age’, and for the children with caries at 1.5 years of age the factor was ‘Tooth brushing with mother's help’.2) We suggest that more efficient dental health guidance should be given at a 1.5-year-old dental health examination.3) The screening method with the eight variables was valid for prediction of caries increment in the children with caries, but not in the children without caries. more...
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- 1993
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15. Changes in Attitude toward Dental Health Behavior and Periodontal Health in Mothers with 18-month-old Children
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Makoto Kawamura, Tatsuji Yamamura, Hisako Sasahara, Masaharu Miyagi, and Yoshifumi Iwamoto
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medicine.medical_specialty ,business.industry ,Dental health ,Medicine ,business ,Psychiatry - Published
- 1993
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16. Maternal and Child Dental Health Care at a Public Health Center. I. Relationship between Life Environment in 1.5-year-olds and Caries Prevalence in 3-year-olds
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Masaharu Miyagi, Masayuki Morishita, Hisako Sasahara, Kitamoto J, Yoshifumi Iwamoto, Makoto Kawamura, Kunio Kawabata, and Nagao M
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Tooth brushing ,Pediatrics ,medicine.medical_specialty ,Multivariate analysis ,business.industry ,Dental health ,Environmental health ,Public health ,Screening method ,Medicine ,Caries prevalence ,business ,Breast feeding - Abstract
The purpose of this study was to extract the factors which affect the caries increment between 1.5 and 3 years of age by dividing the subjects into two groups, one with and the other without caries at 1.5 years of age.The subjects were 1641 children who received a 3-year-old dental health examination at a public health center in Hiroshima City between April 1990 and November 1991.Eight factors associated with caries prevalence at 3 years of age were examined using multivariate analysis. These factors were ‘Family type’, ‘Order of birth’, ‘Interruption of breast feeding’, ‘Bottle feeding at 1.5 years of age’, ‘Regularity of between-meal snacks’, ‘Drinking sugary beverages’, ‘Watching TV at meals’ and ‘Tooth brushing with mother's help’.The results were as follows.1) The factors related to caries increment from 1.5 to 3 years of age for the children without caries at 1.5 years of age were ‘Drinking sugary beverages’, ‘Interruption of breast feeding’, ‘Watching TV at meals’ and ‘Bottle feeding at 1.5 years of age’, and for the children with caries at 1.5 years of age the factor was ‘Tooth brushing with mother's help’.2) We suggest that more efficient dental health guidance should be given at a 1.5-year-old dental health examination.3) The screening method with the eight variables was valid for prediction of caries increment in the children with caries, but not in the children without caries. more...
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- 1992
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17. Effects of Sex Hormones on Chemotaxis of Human Peripheral Polymorphonuclear Leukocytes and Monocytes
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Masaharu Miyagi, Hitoshi Aoyama, Masayuki Morishita, and Yoshifumi Iwamoto
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Adult ,Male ,medicine.medical_specialty ,Time Factors ,Nafoxidine ,Neutrophils ,Monocytes ,Clomiphene ,chemistry.chemical_compound ,Sex hormone-binding globulin ,Cell Movement ,Internal medicine ,medicine ,Humans ,Testosterone ,Progesterone ,Estradiol ,biology ,Chemistry ,hemic and immune systems ,Chemotaxis ,N-Formylmethionine leucyl-phenylalanine ,In vitro ,Peripheral ,N-Formylmethionine Leucyl-Phenylalanine ,Chemotaxis, Leukocyte ,Endocrinology ,biology.protein ,Periodontics ,Female ,Hormone ,medicine.drug - Abstract
The effects of sex hormones on the in vitro chemotaxis of polymorphonuclear leukocytes (PMNs) and monocytes were investigated using fMLP as the chemoattractant. PMNs, monocytes, and plasma were obtained from heparinized peripheral blood of healthy adults. Chemotaxis of PMNs or monocytes treated with sex hormones were tested using 48-well chemotaxis microchambers. The correlation between sex hormone levels in plasma and the chemotactic ability of PMNs from the same donor was also investigated. The chemotaxis of PMNs was enhanced by progesterone, while it was reduced by estradiol. Random migration of PMNs was also enhanced by progesterone and reduced by estradiol. The effect of estradiol on PMN chemotaxis was inhibited by addition of antiestrogens or progesterone. Testosterone did not have a measurable effect on PMN chemotaxis. A significant positive correlation was found between the concentration of progesterone in plasma of females and PMN chemotactic ability in vitro. For males, there was no significant relationship between plasma levels of sex hormones and PMN chemotactic ability. Estradiol and testosterone levels in plasma did not correlate with PMN chemotactic ability. Sex hormones had no effect on the chemotaxis of monocytes. These results suggest that the altered PMN chemotaxis associated with gingival inflammation may be due to the effects of sex hormones. more...
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- 1992
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18. Analysis of oestrogen receptor mRNA by reverse transcriptase-polymerase chain reaction in human periodontal ligament cells
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Masayuki Morishita, Atsushi Shimazu, and Yoshifumi Iwamoto
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Adult ,Pathology ,medicine.medical_specialty ,Periodontal Ligament ,Breast Neoplasms ,Adenocarcinoma ,Biology ,Polymerase Chain Reaction ,law.invention ,stomatognathic system ,Osteogenesis ,law ,Tumor Cells, Cultured ,medicine ,Humans ,Periodontal fiber ,RNA, Messenger ,Oestrogen receptor ,skin and connective tissue diseases ,General Dentistry ,Polymerase chain reaction ,Southern blot ,Messenger RNA ,Osteoblasts ,Cell Differentiation ,RNA-Directed DNA Polymerase ,Cell Biology ,General Medicine ,medicine.disease ,Molecular biology ,Reverse transcriptase ,Gene Expression Regulation, Neoplastic ,Blotting, Southern ,Real-time polymerase chain reaction ,Gene Expression Regulation ,Receptors, Estrogen ,Otorhinolaryngology ,Female - Abstract
Periodontal ligament (PDL) cells have osteoblast-like features and are capable of differentiating into osteogenic cells. As human osteoblasts express oestrogen receptor mRNA, it is possible that PDL cells do so also, but findings have been conflicting. To determine whether they do express oestrogen receptor mRNA, the reverse transcriptase-polymerase chain reaction was performed with two different primers. Cells were obtained from a healthy periodontal ligament of premolar extracted for orthodontic reasons. The human breast adenocarcinoma cell-line MCF7 was used as a positive control. Expression of oestrogen receptor mRNA was detected in PDL cells with one of the primers but with less intensity than in MCF7 cells. Southern hybridization confirmed these results. These findings suggest that PDL cells express oestrogen receptor mRNA at low levels. more...
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- 1999
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19. The clinical effect of lysozyme-containing chewing gum on gingivitis
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Takeshi Watanabe, Hitoshi Aoyama, Yoshifumi Iwamoto, Makoto Kawamura, Masaki Shibata, and Hisako Sasahara
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Meal ,business.industry ,Significant difference ,Dentistry ,Placebo ,Chewing gum ,Placebo group ,Double blind ,Gingivitis ,chemistry.chemical_compound ,chemistry ,Medicine ,medicine.symptom ,Lysozyme ,business - Abstract
The purpose of the present study was to investigate the effect of the chewing gum containing egg-white lysozyme on gingivitis. Sixteen adults aged 18 to 26 years, who exhibited clinically slight gingivitis, were chosen for the study based on preliminary screening. The volunteers were randomly assigned to two groups of eight. They were directed to chew a piece of chewing gum containing 1% egg-white lysozyme or placebo for 5 minutes after each meal during 2 weeks. The experiments were performed in a double blind method. Oral examinations (probing depth, gingival score, bleeding score and plaque score) were carried out on day 1, day 8, and day 15 of the experimental period. All the subjects were instructed to continue their habitual toothbrushing. At the end of the experimental period, the volunteers answered a questionnaire about their subjective feelings and what they thought of the gum. The results were as follows.1) The probing depth, the bleeding score, and the plaque score in both groups decreased at the end of the period.2) The experimental group had a significantly larger decreace in the probing depth and plaque score than the placebo group, but there was not a significant difference in the gingival score between the two groups.3) Concerning the size, hardness, thickness, and taste of the chewing gum, most of the subjects of both groups judged them appropriate and favorable. With respect to the mouth condition, 6 of the placebo group noticed no change, while 4 of the experimental group answered that they felt a decrease in gingival bleeding.These results indicate that the lysozyme-containing chewing gum may be effective in the maintenance of oral health and that this gum may be acceptable to patients with gingivitis. more...
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- 1990
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20. Autoantibodies to REG, a beta-cell regeneration factor, in diabetic patients
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Takako Akiyama, Iwao Takahashi, Takayuki Ikeda, Akiyo Yamauchi, Hiroshi Okamoto, Shin Takasawa, Yasuko Uchigata, Nausheen J. Shervani, Kei Nakagawa, Yoshifumi Iwamoto, H. Takada, Koji Nata, and Naoya Noguchi more...
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Adult ,Male ,medicine.medical_specialty ,Adolescent ,Clinical Biochemistry ,chemical and pharmacologic phenomena ,Type 2 diabetes ,medicine.disease_cause ,Biochemistry ,Autoimmunity ,Islets of Langerhans ,immune system diseases ,Internal medicine ,Diabetes mellitus ,Lithostathine ,medicine ,Diabetes Mellitus ,Humans ,Age of Onset ,Child ,Aged ,Autoantibodies ,Glycoproteins ,Autoimmune disease ,Type 1 diabetes ,biology ,business.industry ,Calcium-Binding Proteins ,Autoantibody ,Type 2 Diabetes Mellitus ,Infant ,hemic and immune systems ,General Medicine ,Middle Aged ,medicine.disease ,Recombinant Proteins ,Endocrinology ,Diabetes Mellitus, Type 1 ,Bromodeoxyuridine ,Diabetes Mellitus, Type 2 ,Child, Preschool ,Immunology ,biology.protein ,Female ,Antibody ,business ,Cell Division - Abstract
Background Regenerating gene (Reg) product, Reg, acts as an autocrine/paracrine growth factor for beta-cell regeneration. The presence of autoimmunity against REG may affect the operative of the regenerative mechanisms in beta cells of Type 1 and Type 2 diabetes patients. We screened sera from Type 1 and Type 2 diabetes subjects for anti-REG autoantibodies, searched for correlations in the general characteristics of the subjects with the presence of anti-REG autoimmunity, and tested the attenuation of REG-induced beta-cell proliferation by the autoanitibodies. Material and methods We examined the occurrence of anti-REG autoantibodies in patients’ sera (265 Type 1, 368 Type 2 diabetes patients, and 75 unrelated control subjects) by Western blot analysis, and evaluated inhibitory effects of the sera on REG-stimulated beta-cell proliferation by a 5′-Bromo-2′-deoxyuridine (BrdU) incorporation assay in vitro. Results Anti-REG autoantibodies were found in 24·9% of Type 1, 14·9% of Type 2 and 2·7% of control subjects (P = 0·0004). There were significant differences between the autoantibody positive and negative groups in the duration of disease in the Type 1 subjects (P = 0·0035), and the age of onset in the Type 2 subjects (P = 0·0274). The patient sera containing anti-REG autoantibodies significantly attenuated the BrdU incorporation by REG (35·6 ± 4·06% of the control), whereas the nondiabetic sera without anti-REG autoantibodies scarcely reduced the incorporation (88·8 ± 5·10%). Conclusion Anti-REG autoantibodies, which retard beta-cell proliferation in vitro, are found in some diabetic patients. Thus, autoimmunity to REG may be associated with the development/acceleration of diabetes in at least some patients. more...
- Published
- 2004
21. Inside Back Cover: Electrical Network of Single-Crystalline Metal Oxide Nanoclusters Wired by π-Molecules (Angew. Chem. Int. Ed. 42/2014)
- Author
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Ryo Tsunashima, Katsuya Ichihashi, Yu-Fei Song, Katsuya Ishiguro, Katsuya Inoue, Tomoyuki Akutagawa, Yusuke Baba, Sadafumi Nishihara, Chisato Kato, and Yoshifumi Iwamoto
- Subjects
Materials science ,Oxide ,Nanoparticle ,Molecular electronics ,Nanotechnology ,General Chemistry ,Catalysis ,law.invention ,Nanoclusters ,Metal ,chemistry.chemical_compound ,chemistry ,law ,visual_art ,Electrical network ,visual_art.visual_art_medium ,Molecule ,Cover (algebra) - Published
- 2014
- Full Text
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22. Innenrücktitelbild: Electrical Network of Single-Crystalline Metal Oxide Nanoclusters Wired by π-Molecules (Angew. Chem. 42/2014)
- Author
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Ryo Tsunashima, Yu-Fei Song, Katsuya Ichihashi, Yoshifumi Iwamoto, Chisato Kato, Yusuke Baba, Tomoyuki Akutagawa, Katsuya Ishiguro, Sadafumi Nishihara, and Katsuya Inoue
- Subjects
Metal ,chemistry.chemical_compound ,chemistry ,law ,visual_art ,Electrical network ,visual_art.visual_art_medium ,Oxide ,Molecule ,Nanotechnology ,General Medicine ,Nanoclusters ,law.invention - Published
- 2014
- Full Text
- View/download PDF
23. The validity and reproducibility of an oral rating index as a measurement of gingival health care and oral hygiene level in adults
- Author
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Makoto Kawamura, Hisako Sasahara, Yoshifumi Iwamoto, Chiaki Inoue, and Setsuko Fukuda
- Subjects
Adult ,Male ,Index (economics) ,Oral Hygiene Index ,viruses ,Bleeding on probing ,Dentistry ,Gingival Pocket ,Oral hygiene ,Gingivitis ,Japan ,Gingival health ,medicine ,Humans ,Aged ,Periodontitis ,Observer Variation ,Reproducibility ,Analysis of Variance ,business.industry ,Reproducibility of Results ,biochemical phenomena, metabolism, and nutrition ,Middle Aged ,medicine.disease ,Periodontics ,Health education ,Female ,medicine.symptom ,Periodontal Index ,business - Abstract
The purpose of this study was to investigate the validity and reproducibility of an oral rating index (ORI) as a conceptual measurement of “gingival health care” level in adults. The study was conducted on 163 patients who received medical examinations and attended for dental checkups at a hospital. The score of the ORI was recorded as excellent (+2), good (+1), questionable (0), poor (−1) or very poor (−2). The validity of the ORI was investigated by comparing it with: Jackson's gingivitis index (GI); Greene & Vermillion's debris index (DI) and calculus index (CI); probing pocket depth (PPD); and percentage of bleeding on probing (%BOP). The intra- and inter-examiner reproducibility of the ORI was assessed on a flat screen using patients' color transparencies of the mouth. The ORI had significantly negative correlations with the GI, DI, CI, and PPD (p more...
- Published
- 2000
24. Present state of dental health knowledge, attitudes/behaviour and perceived oral health of Japanese employees
- Author
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Makoto Kawamura and Yoshifumi Iwamoto
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Adult ,Male ,Toothbrushing ,medicine.medical_specialty ,Health Knowledge, Attitudes, Practice ,business.product_category ,Adolescent ,Health Behavior ,Oral Health ,Disease ,Health Promotion ,Oral health ,Dental Caries ,Occupational safety and health ,Dental Devices, Home Care ,stomatognathic system ,Japan ,Surveys and Questionnaires ,Toothache ,Medicine ,Humans ,Dental Care ,Workplace ,General Dentistry ,Dentures ,Occupational Health ,Periodontal Diseases ,Aged ,Toothpaste ,business.industry ,Dental health ,Age Factors ,Middle Aged ,Patient Acceptance of Health Care ,stomatognathic diseases ,Health promotion ,Family medicine ,Gingival Diseases ,Health Education, Dental ,Health education ,Female ,medicine.symptom ,business ,Gingival Hemorrhage ,Attitude to Health - Abstract
The aim of this survey was to assess the present state of dental health knowledge, attitudes/behaviour and perceived oral health of Japanese employees. A 60-item questionnaire was used in a dental health project in the work place. The subjects comprised 77,845 employees, 76 per cent of whom reported delaying a dental visit until they had toothache, with about 60 per cent delaying even when they discovered a decayed tooth. The majority did not regard decayed teeth as a disease and only a minority reported regular dental visits. About three quarters reported bleeding gums on brushing, although more than half had never been taught professionally how to clean their teeth and less than 5 per cent flossed daily. More than half believed that false teeth were inevitable in old age, and that their teeth were getting worse despite daily brushing. About 70 per cent of the employees thought that it was impossible to prevent gum disease with toothbrushing alone, and nearly half believed a toothpaste with fluoride was effective in preventing periodontal disease. Reorientation of oral health care in Japan, therefore, is urgently needed and dental services have to be provided for the implementation of systematic oral health promotion for employees in the workplace. more...
- Published
- 2000
25. Estradiol enhances the production of mineralized nodules by human periodontal ligament cells
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Afzal Hossain Bachchu, Tatsuji Yamamura, Atsushi Shimazu, Masayuki Morishita, and Yoshifumi Iwamoto
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Adult ,Male ,Adolescent ,medicine.drug_class ,Periodontal Ligament ,Connective tissue ,Anthraquinones ,Culture Media, Serum-Free ,Andrology ,Calcification, Physiologic ,stomatognathic system ,medicine ,Periodontal fiber ,Animals ,Humans ,Regeneration ,Coloring Agents ,Cells, Cultured ,Estradiol ,Chemistry ,Regeneration (biology) ,Anatomy ,medicine.disease ,Alkaline Phosphatase ,Staining ,Culture Media ,medicine.anatomical_structure ,Estrogen ,Spectrophotometry ,Periodontics ,Alkaline phosphatase ,Cattle ,Female ,Fetal bovine serum ,Calcification - Abstract
A primary objective in the treatment of periodontal disease is the regeneration of the mineralized and soft connective tissue. PDL cells produce mineralized nodules in vitro which is one of the important functions of PDL cells for regenerative therapy. The purpose of this study was to investigate the effects of estradiol on mineralized nodule formation by human PDL cells. PDL cells were obtained from healthy donors and maintained in DMEM with 10% fetal bovine serum. Serum-free medium was used when the effects of estradiol were tested. ALP activity in the supernatant of cells disrupted by sonication was analyzed spectrophotometrically. The formation of mineralized nodules was assessed by staining the PDL cells with alizarine red and counting the number of nodules. at Estradiol 20 ng/ml significantly enhanced the ALP activity and mineralized nodule formation compared to the control. These results suggested that estrogen status may modify the regenerative activity of periodontal tissue. more...
- Published
- 1999
26. Effects of sex hormones on production of interleukin-1 by human peripheral monocytes
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Masaharu Miyagi, Yoshifumi Iwamoto, and Masayuki Morishita
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Adult ,Lipopolysaccharides ,Male ,Salmonella typhimurium ,medicine.medical_specialty ,Indomethacin ,Inflammation ,Enzyme-Linked Immunosorbent Assay ,Biology ,Bone resorption ,Dinoprostone ,Monocytes ,Mediator ,Risk Factors ,Internal medicine ,medicine ,Humans ,Cyclooxygenase Inhibitors ,Testosterone ,Prostaglandin E2 ,Periodontitis ,Cells, Cultured ,Progesterone ,Dose-Response Relationship, Drug ,Estradiol ,Interleukin ,Dose–response relationship ,Endocrinology ,Periodontics ,Female ,medicine.symptom ,medicine.drug ,Hormone ,Interleukin-1 - Abstract
Interleukin-1 (IL-1) is a potent mediator of inflammation and is known to induce bone resorption. We studied the effects of sex hormones on the function of human monocytes and demonstrated that prostaglandin E2 (PGE2) production was enhanced by progesterone and estradiol. As PGE2 has been shown to suppress the production of IL-1 by monocytes, it was speculated that sex hormones also modify the production of IL-1 by regulating PGE2 production. Thus, the effects of sex hormones on the production of IL-1 from human peripheral monocytes and the influence of PGE2 were investigated.Mononuclear leukocytes were obtained from 22 healthy adults. Progesterone, 17-beta estradiol (estradiol), and testosterone were used as representative sex hormones. Monocytes were incubated at 37 degrees C in air with 5% CO2 for 24 hours in RPMI 1640 medium with sex hormones at the designated concentrations. LPS (Salmonella typhimurium) was used to stimulate the monocytes at a concentration of 10 microg/ml. The concentrations of IL-1alpha and IL-1beta in the medium were determined by enzyme-linked immunosorbent assay kits. The concentration of PGE2 was determined using a direct radio-immunoassay kit. Indomethacin was used to inhibit the synthesis of PGE2 and eliminate its effect on the production of IL-1.Estradiol at concentrations of 0.04 ng/ml or more significantly reduced both IL-1alpha and IL- 1beta production. Progesterone also reduced IL-1alpha and IL-1beta production significantly at concentrations of 0.1 ng/ml or more and 0.02 ng/ml or more, respectively. The reductions in IL- 1alpha and IL-1beta production by sex hormones were not affected by addition of indomethacin.Estradiol and progesterone inhibited the production of IL-1 from human peripheral monocytes. The inhibition was not the result of enhanced production of PGE2. Under conditions in which sex hormone levels are low, monocytes produce IL- more readily in response to stimulation by LPS than high levels of such hormones. Low concentrations of sex hormones may be considered as one of the risk factors for periodontitis. more...
- Published
- 1999
27. Relationship between mothers' gingival condition and caries experience of their 3-year-old children
- Author
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Hisako Sasahara, Makoto Kawamura, Kunio Kawabata, and Yoshifumi Iwamoto
- Subjects
Adult ,Family Health ,Male ,Analysis of Variance ,Chi-Square Distribution ,business.industry ,DMF Index ,Dentistry ,Mothers ,Dental Caries ,Middle Aged ,Oral Hygiene ,Gingivitis ,stomatognathic diseases ,Japan ,Child, Preschool ,Community health ,Prevalence ,Medicine ,Humans ,Female ,Periodontal Index ,business ,Caries experience ,General Dentistry - Abstract
Objectives. The main aim of this study was to investigate the relationship between mothers’ gingival condition (an assumed indicator of their self-care level) and the prevalence and severity of dental caries of their children. Sample and Methods. Subjects comprised 1471 pairs of mothers and their children who attended for the 3-year-old dental check-up at a community health centre in Hiroshima. The mothers’ gingival condition was scored as ‘excellent (+2)’, ‘good (+1)’, ‘questionable (0)’, ‘poor (−1)’ or ‘very poor (−2)’ according to the criteria of the Oral Rating Index (ORI). Caries experience of the children was recorded using the WHO caries diagnostic criteria for dmft. Results. The percentage of caries-free children was 51·3%, and the mean dmft score was 2·61. The percentages of caries-free were higher and the mean dmft level were lower in the children of mothers with better gingival condition compared to the children of mothers with worse gingival condition. Conclusions. Mothers’ gingival condition was associated with the prevalence and severity of dental caries of their children. more...
- Published
- 1999
28. In vitro activity of tetracyclines, macrolides, quinolones, clindamycin and metronidazole against periodontopathic bacteria
- Author
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Widowati, Kenji Okuda, H. Suginaka, Yoichiro Miyake, Keiko Tsuruda, and Yoshifumi Iwamoto
- Subjects
Ofloxacin ,medicine.drug_class ,Antibiotics ,Antitrichomonal Agents ,Minocycline ,Microbial Sensitivity Tests ,Quinolones ,Aggregatibacter actinomycetemcomitans ,Prevotella intermedia ,Microbiology ,stomatognathic system ,Anti-Infective Agents ,Metronidazole ,medicine ,Naphthyridines ,Porphyromonas gingivalis ,Antibacterial agent ,biology ,Chemistry ,Clindamycin ,Tetracycline ,biology.organism_classification ,Anti-Bacterial Agents ,Erythromycin ,stomatognathic diseases ,Actinobacillus ,Periodontics ,Miocamycin ,Rokitamycin ,medicine.drug ,Fluoroquinolones - Abstract
We re-evaluated several antibiotics including newer ones, for their in vitro killing activity, as well as their inhibitory activity, against clinical isolates of periodontopathic bacteria. Tetracyclines were active against Porphyromonas gingivalis, and were highly active against Prevotella intermedia, but demonstrated only a low killing activity against Actinobacillus actinomycetemcomitans. Rokitamycin, a new macrolide, and clindamycin were highly active against P. gingivalis and P. intermedia, but showed very weak killing activity against A. actinomycetemcomitans. Quinolones demonstrated excellent bactericidal activity against A. actinomycetemcomitans, and good inhibitory and bactericidal activity against P. gingivalis and P. intermedia. Metronidazole had an activity almost equivalent to quinolones against P. gingivalis and P. intermedia; but it was the least active against A. actinomycetemcomitans. more...
- Published
- 1995
29. Effects of fluoride intake on the mineral content, acid solubility and resorption caused by experimental periodontitis of rat alveolar bone
- Author
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Masaharu Miyagi, Yoshifumi Iwamoto, Masayuki Morishita, Makoto Kawamura, and Keiko Tsuruda
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Male ,medicine.medical_specialty ,Pathology ,Alveolar Bone Loss ,Bone resorption ,chemistry.chemical_compound ,Fluorides ,Internal medicine ,medicine ,Alveolar Process ,Animals ,Solubility ,Rats, Wistar ,Periodontitis ,General Dentistry ,Dental alveolus ,Phosphorus ,Cell Biology ,General Medicine ,medicine.disease ,Resorption ,Rats ,Fluoride intake ,Endocrinology ,Otorhinolaryngology ,chemistry ,Distilled water ,Calcium ,Fluoride - Abstract
Adult rats were given either distilled water or drinking water containing 100 parts/10(6) of fluoride. The alveolar bone of rats given fluoride for 90 days showed an increased mineral content and decreased acid solubility compared to the bone of rats given distilled water. Experimental periodontitis was initiated in both groups after 110 days of treatment to cause alveolar bone resorption. Fourteen days later, the rats were killed and it was found that the alveolar bone resorption caused by experimental periodontitis was significantly smaller in the rats given fluoride in their drinking water than in those given distilled water. The findings suggest that fluoride intake might have a protective effect on rapidly progressing alveolar bone resorption. more...
- Published
- 1994
30. Behavioral study on dental caries. Part I. Factor analysis of personalities of mother and child, and types of dental caries
- Author
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Makoto Kawamura, Yoshifumi Iwamoto, Hitoshi Aoyama, and Kazunori Tsuchida
- Subjects
business.industry ,Behavioral study ,Medicine ,Dentistry ,business - Abstract
“齲蝕予防は育児である”という言葉があるように, 乳幼児期における齲蝕には, 家庭環境, 特に母親が大きな影響力を持っていると言われている. 本研究では, 4~6歳の幼稚園児とその母親153組を対象に, 母子の性格と齲蝕との関連性について検討した。母子の性格検査は, 母親に対しては“YG性格検査用紙”を, 園児に対しては高木・坂本の“幼児・児童性格診断検査用紙”を使用して行った。また, 園児の齲蝕罹患状況については, 厚生省の分類に従い齲蝕罹患型 (O・A・B・C型) に分けたものを用いて以下に示す結果を得た。1) 因子分析法を適用し, 母子の性格特性 (上記性格検査の22項目) が, 4つの共通因子によってほぼ説明しうることがわかった。第1因子は子供安定性の因子, 第2因子は母親消極性の因子, 第3因子は母親不安定・不適応性の因子, 第4因子は子供社会性の因子と名付けられた。2) 母親消極性の因子 (第2因子), 子供社会性の因子 (第4因子) と齲蝕罹患型との関連性は認められなかった。一方子供安定性の因子 (第1因子) については, さらに検討が必要と思われた。3) 性格的にはむしろ問題と思われる不安定・不適応性 (第3因子) の高い母親の場合, かえって齲蝕予防の実践が効果的に行われていることが示唆された。 more...
- Published
- 1985
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31. Studies on evaluation of masticatory function during initial treatment of periodontal disease. A method for the measurement of biting force
- Author
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Yoshifumi Iwamoto, Masayuki Morishita, and Hisako Iwasaki
- Subjects
Periodontal disease ,business.industry ,Initial treatment ,Dentistry ,Medicine ,business ,Masticatory force - Published
- 1986
- Full Text
- View/download PDF
32. Studies on Iodide Peroxidase in Human Parotid Saliva
- Author
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Tatsuo Watanabe, Akira Tsunemitsu, Aiki Yasutake, and Yoshifumi Iwamoto
- Subjects
Saliva ,biology ,Biochemistry ,Chemistry ,Iodide Peroxidase ,biology.protein ,Halogenation ,Parotid saliva ,Tyrosine ,Peroxidase - Published
- 1977
- Full Text
- View/download PDF
33. Dental behavioral science. Part I. The assessment of oral health status by factor analysis
- Author
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Yoshifumi Iwamoto and Makoto Kawamura
- Subjects
Male ,Gerontology ,business.industry ,Age Factors ,Behavioural sciences ,Oral Health ,Oral health ,Oral Hygiene ,Japan ,Environmental health ,Humans ,Medicine ,Female ,Dental Health Surveys ,business - Published
- 1984
- Full Text
- View/download PDF
34. Bacteriological study of gingivitis in pubertal children. II. Subgingival microflora cultivable on the nonselective blood agar medium
- Author
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Masaharu Miyagi, Yoshifumi Iwamoto, Hitoshi Aoyama, Masayuki Morishita, and Keiko Tsuruda
- Subjects
Agar plate ,stomatognathic diseases ,Gingivitis ,biology ,Actinomyces spp ,medicine ,Actinomyces naeslundii ,Bacteroides ,medicine.symptom ,Capnocytophaga ,biology.organism_classification ,Actinomyces israelii ,Microbiology - Abstract
The present study involved cultural and microscopic characterization of the subgingival microorganisms of pubertal children with gingivitis. Samples were obtained from eight children 12-14 years of age. Gram-positive organisms made up 56% of the isolates and included mainly Actinomyces israelii, Actinomyces naeslundii. Gram-negative anaerobic rods constituted 25% of the isolates and Bacteroides intermedius (9.7%) was most predominant species. The selective media permitted significantly lower recovery of Actinomyces spp. and Capnocytophaga spp. than nonselective blood agar medium. However, sixty-nine percents of isolates were cultured on the selective media employed in the present study. These results suggest that the subgingival microflora of gingivitis in pubertal children may be similar to those of adult gingivitis and that the use of these six selective media makes it possible to enumerate predominant cultivable microorganisms in subgingival area of pubertal children with gingivitis. more...
- Published
- 1989
- Full Text
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35. Periodontal health in high school. Survey of periodontal disease in junior high school
- Author
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Hitoshi Aoyama, Hisako Iwasaki, Yoshifumi Iwamoto, Makoto Kawamura, Masayuki Morishita, Kazunori Tsuchida, and Masaharu Miyagi
- Subjects
medicine.medical_specialty ,Periodontal disease ,business.industry ,Family medicine ,medicine ,Physical therapy ,business ,School survey - Published
- 1986
- Full Text
- View/download PDF
36. Field Work on the New Caries Activity Test Solution, S-3105
- Author
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Masana Shinmoto, Tatsuo Watanabe, Kyoji Toda, Masakazu Nakamura, and Yoshifumi Iwamoto
- Subjects
Azides ,Sucrose ,DMF Index ,business.industry ,Dentistry ,Dental Caries ,Caries activity ,Test (assessment) ,Drug Combinations ,Child, Preschool ,Test score ,Oxazines ,Dental Caries Activity Tests ,Humans ,Medicine ,Sodium Azide ,business ,Test solution - Abstract
A caries activity test solution, S-3105 developed by Nakamura et al., was examined in the field.Two hundred and thirty pre-school children, ranging in age from 3 to 5 years, were studied. Three dentists examined decayed, missing, and filled teeth, and debris score. Buccal plaque was sampled by swabbing with cotton, and then incubated in the solution at 37°C for 30 min. The color change was estimated as the test score. Then the relationship between oral status and the test was statistically analyzed.The test score showed positive significant correlations with d ratio, dmf, caries severity index (CSI), and ΔCSI.This solution seemed useful for caries activity tests in the field. more...
- Published
- 1980
- Full Text
- View/download PDF
37. Fluorine in food. I. Determination of total fluoride in powdered milk for babies
- Author
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Yoshifumi Iwamoto, Masaharu Miyagi, Naoya Tsubakida, and Takeshi Watanabe
- Subjects
chemistry.chemical_compound ,Chemistry ,Environmental chemistry ,Fluorine ,chemistry.chemical_element ,Food science ,Fluoride - Abstract
The purpose of this study was to determine total fluoride in powdered milk for babies. We determined fluoride in the diffusates of ashed samples using an ion-specific electrode.The 74 blanks observed in this study contained 0.10±0.10μg of fluoride (M±SD). Mean recovery for analysis determined by adding 0.1-1.0μg of fluoride was 94%. Coefficients of variance in recovery test and in repeated analysis ranged from 5 to 13%, 4 to 8%, respectively.The total fluoride content of powdered milk ranged from 0.22 to 0.40ppm. more...
- Published
- 1989
- Full Text
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38. [Untitled]
- Author
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Makoto KAWAMURA, Hisako IWASAKI, Masayuki MORISHITA, Tatsuo WATANABE, Takao MAKISHIMA, Kiyoshi MAEKAWA, and Yoshifumi IWAMOTO
- Published
- 1983
- Full Text
- View/download PDF
39. Study of a New Caries Activity test. In vitro Test of the Test Solution S-3105
- Author
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Yoichi Yamamoto, Masakazu Nakamura, and Yoshifumi Iwamoto
- Subjects
Azides ,Sucrose ,In vitro test ,Dental Plaque ,Dentistry ,Dental Caries ,In Vitro Techniques ,Streptococcus mutans ,chemistry.chemical_compound ,Oxazines ,Dental Caries Activity Tests ,Humans ,Food science ,Sodium Azide ,business.industry ,Resazurin ,Caries activity ,Acid production ,Test (assessment) ,Highly sensitive ,Drug Combinations ,chemistry ,Child, Preschool ,Sodium azide ,business ,Test solution - Abstract
Many caries activity tests have been devised and developed, but they have some disadvantage for routine use. In the present study, we attempted to develop a new caries activity test which everyone could use conveniently and rapidly.Various test solutions which were composed of pH indicators, carbohydrates, and anti-microbial agents were prepared. The test solutions were inoculated with cariogenic microorganisms such as St. mutans and pH change or color change of test solutions were measured.Among the test solutions, S-3105, which was composed of resazurin, sucrose, and sodium azide, could detect the acid production of cariogenic microorganisms clearly in 30 min. Color change and pH change of the test solution S-3105 were parallel and the color changed from bluish violet to reddish violet and then to pink. S-3105 was highly sensitive to St. mutans and L. casei but not to other microoraganisms.In the experiment using human dental pleque, a statistically significant correlation was found between the test results of S-3105 and Cariostat or the Snyder test. Also, in a clinical study with 27 children (3 to 5 years old), significant correlations were found between the test results and caries indices such as def (%) and caries severity index (CSI) as well as the results of Cariostat.From the above results, the test solution S-3105 was found to be able to detect the acid production of cariogenic microorganisms visually in 30 min. This test solution might be useful for diagnosis of caries activity and might also be used advantageously for educational and motivational purposes. more...
- Published
- 1980
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40. Inhibition of coenzyme Q10-enzymes, succinoxidase and NADH-oxidase, by adriamycin and other quinones having antitumor activity
- Author
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Thomas H. Porter, Inge L. Hansen, Karl Folkers, and Yoshifumi Iwamoto
- Subjects
Naphthacenes ,Macromolecular Substances ,Ubiquinone ,Cellular respiration ,Daunorubicin ,Biophysics ,Pharmacology ,Biochemistry ,Electron Transport ,Structure-Activity Relationship ,chemistry.chemical_compound ,polycyclic compounds ,medicine ,NADH, NADPH Oxidoreductases ,Doxorubicin ,Colloids ,Molecular Biology ,Phospholipids ,Lapachol ,chemistry.chemical_classification ,Coenzyme Q10 ,Alanine ,Antibiotics, Antineoplastic ,biology ,Myocardium ,Succinate dehydrogenase ,Quinones ,Cell Biology ,Mitochondria, Muscle ,Succinate Dehydrogenase ,carbohydrates (lipids) ,Kinetics ,Aminoglycosides ,Enzyme ,chemistry ,Coenzyme Q – cytochrome c reductase ,biology.protein ,medicine.drug - Abstract
Summary Adriamycin, carminomycin, and daunorubicin inhibit the coenzyme Q 10 -enzymes, succinoxidase and NADH-oxidase. Adriamycin 14-octanoate, which is more lipoidal than adriamycin, was the most effective inhibitor of the anthracyclines for both enzymes, and was 1/12 as effective as the standard inhibitor, 6-ω-cyclohexylpentyl-5-hydroxy-2,3-dimethoxy-1,4-benzoquinone, of coenzyme Q 10 for NADH-oxidase. Lapachol and dichloroallyl lawsone inhibited succinoxidase, and the latter of all quinones was second only to the standard for inhibition. These data indicate that the antitumor activities of adriamycin could possibly be partly due to inhibition of CoQ 10 -enzymes in electron transfer processes of cell respiration in addition to intercalation within DNA helices. more...
- Published
- 1974
- Full Text
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41. [Untitled]
- Author
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Tatsuo WATANABE, Kyoji TODA, Masayuki MORISHITA, Masana SHINMOTO, and Yoshifumi IWAMOTO
- Published
- 1982
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42. Absence of coenzyme Q10 as an intrinsic component of aldehyde oxidase
- Author
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John T. Romeo, Karl Folkers, Yoshifumi Iwamoto, and Vattana Pinphanichakarn
- Subjects
chemistry.chemical_classification ,Coenzyme Q10 ,Binding Sites ,Ubiquinone ,Biophysics ,Cell Biology ,Aldehyde Oxidoreductases ,Biochemistry ,Chromatography, DEAE-Cellulose ,chemistry.chemical_compound ,Enzyme ,Liver ,chemistry ,Coenzyme Q – cytochrome c reductase ,Animals ,Chromatography, Thin Layer ,Rabbits ,Molecular Biology ,Aldehyde oxidase ,Protein Binding - Abstract
Many investigators have purified an aldehyde oxidase from mammalian livers, and described reactions of this enzyme with diverse substrates. Coenzyme Q 10 was unambiguously identified and found present in some of these enzyme preparations. It was considered that coenzyme Q 10 might participate in the functionality of this enzyme, but the validity of the intrinsic association of coenzyme Q 10 was questioned. We have similarly purified aldehyde oxidase from rabbit livers. No coenzyme Q 10 could be detected under controlled conditions for detecting the presence of coenzyme Q 10 . It is concluded that coenzyme Q 10 may be a contaminant of some aldehyde oxidase preparations, and that it is not intrinsic for the functionality of this enzyme. more...
- Published
- 1974
- Full Text
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43. Correlations Between Salivary Protease and Supragingival or Subgingival Calculus Index
- Author
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Kyoji Toda, Masayuki Morishita, Yoshifumi Iwamoto, and Tatsuo Watanabe
- Subjects
Adult ,Male ,0301 basic medicine ,Saliva ,Adolescent ,Gingival and periodontal pocket ,medicine.medical_treatment ,education ,Dentistry ,Microbiology ,03 medical and health sciences ,0302 clinical medicine ,medicine ,Humans ,Periodontal Pocket ,Dental Calculus ,Child ,General Dentistry ,Protease ,Chemistry ,business.industry ,Calculus (dental) ,Subgingival calculus ,030206 dentistry ,Hydrogen-Ion Concentration ,Middle Aged ,medicine.disease ,stomatognathic diseases ,030104 developmental biology ,Female ,Periodontal Index ,business ,Peptide Hydrolases - Abstract
Significant positive correlations between protease activities in saliva and supragingival/subgingival calculus indices, periodontal pocket depth, or P-M-A index were confirmed. When the partial correlation was calculated, the subgingival calculus index correlated well with the sediment protease at pH 8.5, and the supragingival calculus index showed a significant correlation with the supernate protease at pH 4.5. more...
- Published
- 1982
- Full Text
- View/download PDF
44. Behavioral dental science. Part VIII. The dentist's rating and adolescent's perceptions of oral health
- Author
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Hisako Sasahara, Kazunori Tsuchida, Hitoshi Aoyama, Yoshifumi Iwamoto, Nagao M, and Makoto Kawamura
- Subjects
Adolescent ,Attitude of Health Personnel ,business.industry ,Dental health ,media_common.quotation_subject ,Dentists ,Dentistry ,Oral Health ,Cognition ,Oral health ,Test (assessment) ,stomatognathic diseases ,Perception ,Health Education, Dental ,Humans ,Oral examination ,Medicine ,business ,Attitude to Health ,media_common - Abstract
The purpose of this study was twofold. The first objective was to measure the periodontal status and perceptions of oral health in adolescents. The second objective was to determine the effects that a program of dental health education would have on their periodontal status and perceptions of oral health. The dental health education with a 90-minute lecture had been given to eleventh-grade students (352) in the previous year. The dental health education program was not carried out in tenth-grade students (442) yet. The oral examination was performed by a dentist for both grades. The examination time was about ten seconds per person. The students' periodontal status was scored as "excellent (+2)," "good (+1)," "questionable (0)," "poor (-1)," or "very poor (-2)" according to the criteria of an Oral Rating Index (ORI) System proposed by the present authors. The level of students' perceptions was assessed by the cognition score of our dental health test (10 items concerning dental attitudes and behavior). Main results were as follows: 1. Only 6% of the 10-th grade students were judged as having "excellent (+2)" oral health. Thirty-six percent were "very poor (-2)" or "poor (-1)" periodontal status. 2. The mean cognition score and the mean ORI score of the 11-th grade students were significantly greater for each sex group than those of the 10-th grade students. 3. The cognition score showed a clear relationship to the ORI score in each grade and in all. The behavioral component of the cognition score was more closely related to the ORI score. These findings suggest that the dental health education contributes to the improvement of periodontal status as well as that of perceptions. Furthermore, the ORI may be utilized for a screening system to evaluate adolescents' periodontal status quickly and effectively. more...
- Published
- 1988
- Full Text
- View/download PDF
45. Dental health service in adults. I. Tooth-brushing habits and periodontal condition evaluated by CPITN
- Author
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Yuzo Fujita, Jiro Tsujikawa, Koji Konishi, Ryo Nakamura, Teruo Matsumura, Kenji Kusunoki, Yoko Ogawa, Yoshifumi Iwamoto, and Tatsuo Watanabe
- Subjects
Orthodontics ,Periodontal tissue ,business.industry ,Dental health ,Dentistry ,medicine.disease ,Dental examinations ,stomatognathic diseases ,stomatognathic system ,Age groups ,Dental examination ,Oral and maxillofacial pathology ,medicine ,Christian ministry ,Once daily ,business - Abstract
The present investigation was designed to develop effective dental health practices in adults at their place of work. Their periodontal condition was examined by CPITN, and the relationships between it and their brushing habits, frequency of dental examination, and subjective symptoms of their gingiva were determined in both male and female subjects. The results are as follows:1. Females needed less dental treatment of their periodontal tissues than males; the difference was statistically significant in the age groups of 30-40 and 45-65.2. In all age groups, females brushed their teeth more times daily than males: females brushed an average of over three times daily, and few of them brushed less than once daily. The difference between females and males was statistically significant. The frequency of daily brushing by all of the adults in the present survey was higher than that found by the Ministry of Health and Welfare dental disease survey conducted in 1981.3. In all age groups the periodontal condition was better in subjects who brushed three times a day than in those who brushed only once daily. These results suggest the importance of good tooth-brushing habits for a good periodontal condition.4. Employees in businesses that provided frequent dental examinations had healtheir periodontal tissues than those in businesses that provided only one dental examination. This tendency was the same even when the data were categorized by sex or region.5. There was a relationship between the condition of periodontal tissues and subjective symptoms of gingiva obtained from questionaires. The periodontal tissue of participants who described subjective gingival symptoms was in worse condition than that of those who never felt any gingival abnormality, and the difference in periodontal condition between the two groups was significant.These findings suggest that dental health practices conducted regularly are important for adults. more...
- Published
- 1987
- Full Text
- View/download PDF
46. Coenzyme Q. 167. Antimetabolites of coenzyme Q. 19. Synthesis and bioassays of new dimethoxyalkylmercapto-1,4-benzoquinones
- Author
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Thomas H. Porter, Yoshifumi Iwamoto, Ronald J. Wikholm, Conny Bogentoft, and Karl Folkers
- Subjects
Chemistry ,Drug Discovery ,Molecular Medicine ,Organic chemistry ,Bioassay - Published
- 1974
- Full Text
- View/download PDF
47. Purification and Amino Acid Analysis of Human Parotid Saliva Lysozyme
- Author
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Ryo Nakamura, Tatsuo Watanabe, Akira Tsunemitsu, and Yoshifumi Iwamoto
- Subjects
0301 basic medicine ,Serine ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,stomatognathic system ,Humans ,Parotid Gland ,Amino Acids ,Tyrosine ,Saliva ,General Dentistry ,Chromatography ,Chemistry ,Tryptophan ,030206 dentistry ,Glutamic acid ,Electrophoresis, Disc ,Molecular Weight ,stomatognathic diseases ,Electrophoresis ,030104 developmental biology ,Biochemistry ,Muramidase ,Specific activity ,Ultracentrifuge ,Lysozyme ,Ultracentrifugation - Abstract
Lysozyme from human parotid saliva was purified to the point where it was judged, by the criteria of ultracentrifugation and disk electrophoresis, to be homogeneous. The parotid lysozyme had a specific activity 3.5 times greater than that of hen egg-white lysozyme and a composition that was different, especially in regard to tyrosine, serine, and glutamic acid content. The molecular weight was determined to be around 14,300. When tryptophan residues were modified with N-bromosuccinamide, no lytic activity was found, which indicates that the tryptophan residues may play an important role in the maintenance of the parotid lysozyme activity. more...
- Published
- 1970
- Full Text
- View/download PDF
48. Nucleotidase Activity of the Purified Acid Phosphomonoesterase from Human Parotid Saliva
- Author
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Tatsuo Watanabe, Ryo Nakamura, Yoshifumi Iwamoto, and Akira Tsunemitsu
- Subjects
Ions ,chemistry.chemical_classification ,Chromatography ,Nucleotidase activity ,Esterases ,Mercury ,Hydrogen-Ion Concentration ,Tartrate ,Sodium tartrate ,Metal ,Fluorides ,chemistry.chemical_compound ,Enzyme ,chemistry ,Nucleotidases ,Nucleotidase ,visual_art ,Sodium fluoride ,visual_art.visual_art_medium ,Humans ,Parotid Gland ,Parotid saliva ,Saliva ,Tartrates ,General Dentistry - Abstract
Purified acid phosphomonoesterase from human parotid saliva has been characterized as an acid nucleotidase of broad specificity. The enzyme exhibited a pH optimum at 5.8 with 5'-AMP and was not metal dependent. Activity was inhibited greatly by mercuric ions, sodium fluoride, and sodium tartrate. The tartrate inhibition was competitive. more...
- Published
- 1971
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49. STUDIES ON PHOSPHOMONOESTERASE IN HUMAN PAROTID SALIVA
- Author
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Tatsuo Watanabe, Ryo Nakamura, Akira Tsunemitsu, and Yoshifumi Iwamoto
- Subjects
Chemistry ,Phosphomonoesterase ,Physiology ,Parotid saliva - Published
- 1968
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- View/download PDF
50. Effect of a protease derived from the oral bacterium Bacteroides loescheii on the inhibition of calcium-phosphate precipitation by human parotid saliva
- Author
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Tatsuo Watanabe, Masayuki Morishita, Tokumoto K, and Yoshifumi Iwamoto
- Subjects
Calcium Phosphates ,Proteases ,Microorganism ,medicine.medical_treatment ,chemistry.chemical_element ,Biology ,Calcium ,Microbiology ,stomatognathic system ,Mole ,medicine ,Bacteroides ,Humans ,Parotid Gland ,Saliva ,General Dentistry ,Protease ,Chromatofocusing ,Calculus (dental) ,Cell Biology ,General Medicine ,medicine.disease ,biology.organism_classification ,stomatognathic diseases ,Otorhinolaryngology ,chemistry ,Biochemistry ,Bacteria ,Peptide Hydrolases - Abstract
A protease from the culture fluid of this microorganism was purified and characterized using DEAE-Sephadex and chromatofocusing and classified as a thiol protease with a mol. wt estimated as 27,500. It abolished the inhibitory activity of parotid saliva on the precipitation of calcium phosphate from supersaturated solutions. Such proteases may contribute to dental calculus formation. more...
- Published
- 1986
- Full Text
- View/download PDF
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