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Abstract Molecular ferroelectrics have garnered significant attention due to their structural tunability, low synthesis temperature, and high flexibility. Herein, we successfully synthesized imidazole perchlorate (ImClO4) single crystals and high-quality, highly-oriented thin films on Si substrates. These films demonstrated a high inverse piezoelectric coefficient of 55.7 pm/V. Two types of domain bands were observed: type-I bands tilted ~60° relative to the horizontal axis, and type-II bands positioned perpendicular to the horizontal axis. Under a + 20 V bias, type-I bands showed a reduction and detachment of 180° domain walls to form a needle-like domain. It extended toward the band boundary after applying −20 V bias, which grew along the boundary upon contact. In contrast, type-II bands showed straight domain wall motion and displayed a higher piezoresponse than type-I bands. The growth of high quality molecular ferroelectric thin films on Si substrates paves the way for the development of on-chip devices.

Red pepper (Capsicum annuum var. conoides) is commonly used for dried pepper production in China, and the drying process, particularly the during duration, profoundly affects its quality. The findings indicate that prolonged exposure to high temperatures during thermal drying results in significant darkening, an evident decrease in red and yellow tones, and gradual transformation of the pepper's microscopic structure from granular to compact, along with 88% reduction in moisture content and 81% decrease in thickness. The capsaicinoid content increased, resulting in a 4.3-fold increase in spiciness after drying compared to that of fresh pepper. The pepper aroma shifts from fruity, choking, and grassy to herb, dry wood, and smoky. Compounds such as 2-Acetylfuran, furfural, 2-methylfuran, 1-methylpyrrole, 2-methylpyrazine, and 2,5-dimethylpyrazine exhibited positive correlations with drying time, whereas ethyl 2-methylpropanoate, ethyl butanoate, ethyl 2-methylbutanoate, ethyl hexanoate, and 3-methylbutyl butanoate showed negative correlations, indicating their potential as markers for monitoring thermal drying processes.

IntroductionThe increasing penetration of distributed generation (e.g., solar power and wind power) in the energy market has caused unpredictable disturbances in power systems and accelerated the application of intelligent control, such as reinforcement learning (RL), in automatic generation control (AGC). However, traditional RL cannot ensure constraint safety during training and frequently violates the constraints (e.g., frequency limitations), further threatening the safety and stability of grid operation.MethodsTo address the safety issue, we propose a novel safe RL framework that combines expert experiences with the RL controller to achieve imitation-based RL. This method allows an initialized safe policy by imitating expert experiences to prevent random explorations at the beginning. Specifically, we first formulate the AGC problem mathematically as a Markov decision process. Then, the imitation mechanism is developed atop a soft actor–critic RL algorithm.Results and discussionFinally, numerical studies are conducted with an IEEE 39-bus network, which show that the proposed method satisfies the frequency control performance standard better and improves the RL training efficiency.

Unsustainable straw treatment methods detrimentally affect the environment and ecology. Aerobic fermentation (AE) and anaerobic fermentation (AN) are environmentally friendly treatments that better utilise straw resources. In this study, high-throughput sequencing was used to investigate the effects of AE and AN on nutrient content and microbial community structure during tomato straw fermentation. Nitrate nitrogen, available phosphorus, available potassium, and fulvic acid contents following AE were 1250.04 mg/kg, 80.34 %, 161.39 %, and 49.31 %, respectively, which were higher than those following AN. Ammonium nitrogen, humic acid, and humic substance levels following AN were 309.07 %, 31.18 %, and 17.38 %, respectively, which were higher than those following AE. Firmicutes (24.76 %) and Actinobacteria (12.93 %) were more abundant following AE, whereas Proteobacteria (33.82 %) and Bacteroidetes (33.82 %) exhibited higher abundance following AN. AE more effectively eliminated pathogenic bacteria (22.01%–0.26 %) and encouraged stronger interactions between dominant bacterial genera. Redundancy and Mantel test analyses revealed that electrical conductivity and temperature were the most important environmental factors affecting bacterial communities in AE and AN, respectively. AE had a stronger effect on effective nutrient release from tomato straw, implying its greater application potential as a fertiliser. Overall, our study provides a theoretical basis for the optimisation of fermentation methods and processes.

Abstract In recent years, interdisciplinary research has become increasingly popular within the scientific community. The fields of materials science and chemistry have also gradually begun to apply the machine learning technology developed by scientists from computer science. Graph neural networks (GNNs) are new machine learning models with powerful feature extraction, relationship inference, and compositional generalization capabilities. These advantages drive researchers to design computational models to accelerate material property prediction and new materials design, dramatically reducing the cost of traditional experimental methods. This review focuses on the principles and applications of the GNNs. The basic concepts and advantages of the GNNs are first introduced and compared to the traditional machine learning and neural networks. Then, the principles and highlights of seven classic GNN models, namely crystal graph convolutional neural networks, iCGCNN, Orbital Graph Convolutional Neural Network, MatErials Graph Network, Global Attention mechanism with Graph Neural Network, Atomistic Line Graph Neural Network, and BonDNet are discussed. Their connections and differences are also summarized. Finally, insights and prospects are provided for the rapid development of GNNs in materials science at the atomic scale.

Polyvinylidene fluoride (PVDF) and its copolymers present extensive application prospects, especially in the field of wearable electronics. However, utilizing nanofillers for enhanced β‐phase and piezoelectric properties faces challenges like noncontinuous interfaces, poor compatibility between nanofillers and PVDF matrix, and the requirement of high‐voltage polarization, hindering extensive domain alignment on a large scale. Herein, a method is proposed to synthesize high‐performance PVDF composites by introducing hydroxylated barium titanate (H@BTO) nanoparticles and a directional freeze‐drying method to enhance β‐phase content and piezoelectric properties without polarization. Molecular dynamics simulations reveal robust binding interactions between Ba and F atoms along with OH surface terminations on H@BTO, facilitating hydrogen bonding within the PVDF matrix, resulting in dipole alignment and increased spontaneous polarization. The composite film achieves an 86.69% β phase content and a piezoelectric coefficient of ≈14.49 pm V−1 without electric polarization. The freeze‐drying PVDF‐H@BTO composite film paired with a PA6 membrane is used to fabricate triboelectric nanogenerator, demonstrating a current density of ≈107.5 mA m−2 and an output voltage of ≈832 V. Results demonstrate that the utilization of strong binding interactions between various atoms, the hydroxyl anchoring effect, and directional freeze‐drying method as a strategy holds promising prospects for synthesizing high‐performance piezoelectric composites.

Tetracyclines (TCs) are the most commonly antimicrobial agents that used in livestock production worldwide. It is important to supervise tetracyclines residues in food for environmental monitoring and food safety. In this study, a novel, label-free chemiluminescence (CL) assay without antibody was established. Fe3O4 NPs could facilitate the CL interaction between luminol and H2O2. Interestingly, TCs could enhance the catalytic ability of Fe3O4 NPs and result in a further amplification of the CL intensity. The CL intensity varied linearly with the concentration of tetracycline (TC), oxytetracycline (OTC), chlortetracycline (CTC), and ranging from 10–2400, 10–2800, and 5–2100 nmol/L, respectively; The limits of detection were 4 nmol/L for TC, 6 nmol/L for OTC, and 2 nmol/L for CTC. This CL strategy was applied successfully in testing three TCs residues in milk, eggs and honey samples with more sensitive results, which provided an alternative strategy for monitoring the correct use of TCs.

Abstract Electric field-induced second harmonic generation allows electrically controlling nonlinear light-matter interactions crucial for emerging integrated photonics applications. Despite its wide presence in materials, the figures-of-merit of electric field-induced second harmonic generation are yet to be elevated to enable novel device functionalities. Here, we show that the polar skyrmions, a topological phase spontaneously formed in PbTiO3/SrTiO3 ferroelectric superlattices, exhibit a high comprehensive electric field-induced second harmonic generation performance. The second-order nonlinear susceptibility and modulation depth, measured under non-resonant 800 nm excitation, reach ~54.2 pm V−1 and ~664% V−1, respectively, and high response bandwidth (higher than 10 MHz), wide operating temperature range (up to ~400 K) and good fatigue resistance (>1010 cycles) are also demonstrated. Through combined in-situ experiments and phase-field simulations, we establish the microscopic links between the exotic polarization configuration and field-induced transition paths of the skyrmions and their electric field-induced second harmonic generation response. Our study not only presents a highly competitive thin-film material ready for constructing on-chip devices, but opens up new avenues of utilizing topological polar structures in the fields of photonics and optoelectronics.

Objective: Based on software engineering principles, we designed and implemented a medical device data dictionary system that aligns with data integration and industry standards to fulfill the data management needs of medical institutions and healthcare professionals. Materials and Methods: Utilizing the web-based framework alongside open-source technologies such as Spring Boot, Spring Security, Apache MyBatis, Vue, and Element UI, along with database technologies such as MySql, MongoDB, and Redis, we analyze the basic elements of medical device data dictionary system and incorporate layered architecture technology, design the system architecture, functional workflows and database model required for the dictionary system, and conduct professional testing and trial evaluation of the system. Results and Analysis: In this study, we constructed a web-based open medical device data dictionary system that supports front-end and back-end operations. Using a ventilator as a sample for non-integrity assessment, the results of the system utility test showed that the system is functionally complete, satisfies the compatibility requirements of mainstream browsers and poses no significant risks or vulnerabilities to system operation. Conclusion: A medical device data dictionary that characterizes life cycle usage attributes based on their structural and functional properties was constructed. It provides a unified and standardized base for the management and analysis of medical device data, and helps realize the interaction and exchange of medical device data between disparate medical information systems.

The design of LDMOS (Lateral double diffused metal oxide semiconductor) devices with CFP (Contact field plate) has been of great significance in recent years, according to its advantages of low resistance and high switch efficiency. In this paper, this ultra-low $R_{\mathrm{ on,sp}}$ (Specific on-resistance) LDMOS device is simulated, designed, and fabricated. The effects on FOM (Figures-of-merits) characters from physical dimensions, including field plate length ${L}$ , field plate thickness ${H}$ , and slot contact width ${W}$ , have been analyzed and discussed. The best device structure is proposed through simulation, and a related fabrication process is introduced correspondingly. Finally, the electrical measurement results show that $R_{\mathrm{ on,sp}}$ can achieve as low as 6.9m $\Omega \cdot $ mm2 when the source-drain ${BV}$ (Breakdown voltage) arrives at 34.1V, which is improved by 47.1% compared with conventional devices. Furthermore, the TLP (Transmission line pulse) test results indicate that the device owns an ideal SOA (Safe operation area) from both typical devices (W $=\,\,10~\mu \text{m}$ ) and very large devices (W = 2mm).

【Objective】The study presents a systematic review of data on the primary features and turnover of control varieties in regional trials of rice varieties conducted in Guangdong Province. It aims to clarify the progress made in rice breeding in Guangdong and to provide references for rice breeding practices in the double-season rice area of South China.【Method】Based on the related data of control varieties used in regional trials of rice varieties from 2006 to 2022, statistics on variations in control varieties were conducted, and the significant traits such as yield, quality traits and disease resistance of these varieties were analyzed.【Result】Throughout the 16-year regional trial period, a total of 31 control varieties were utilized, the majority of which demonstrated high yield, extensive adaptability and resistance to rice blast disease. Fifteen of the control varieties could be promoted and planted outside Guangdong Province, eight conventional rice control varieties have been widely planted in Guangdong Province, and three hybrid rice control varieties have been recognized as super rice by the Ministry of Agriculture and Rural Affairs. The average yields of the conventional rice control varieties in early and late seasons of 2022 were 7 176.1±243.6 kg/hm2 and 6 500.9±117.08 kg/hm2, respectively. While the hybrid rice control varieties had average yields of 7 282.0±358.6 kg/hm2 and 6 835.8±175.2 kg/hm2 in the early and late seasons, respectively. Twenty-seven control varieties exhibited resistance and moderate resistance to rice blast, while eight control varieties demonstrated resistance and moderate resistance to leaf blight. On average, control varieties were replaced every five years, with the recently replaced control varieties exhibiting enhancements in yield and quality. The average yields of the control varieties increased by 301.8-417.0 kg/hm2 in the early season and by 778.20-966.15 kg/hm2 in the late season during the 16-year trials. The overall quality of the control variety in 2022 was significantly enhanced compared to that in 2006. Specifically, the grain length-breadth ratio increased from 2.76 to 3.25, the average chalkiness decreased from 13.4% to 9.6%, the gel consistency increased from 57.5 mm to 66.2 mm, and the head rice rate increased from 58.06% to 60.51%. The average amylase content of the control variety decreased to 1.2%-5.6% from 2012.【Conclusion】The study analyzes the use of control varieties in regional trials of rice varieties conducted in Guangdong Province from 2006 to 2021. The findings suggest that the selected control varieties demonstrate outstanding overall quality in Guangdong Province. With the improvement of rice production levels, the control varieties with higher yield and better quality are replaced in time and the best ones are selected from excellent varieties in the process of approval. In this way, the yield of new variety is steadily increased and the quality traits are improved, which is more in line with the requirements of production development

The xyloglucan endotransglucosylase/hydrolase (XTH) family is an important multigene family in plants that plays a key role in cell wall reconstruction and stress tolerance. However, the specific traits of XTH genes and their expression patterns under different stresses have not been systematically studied in melon. In this study, based on the genomic data of Cucumis melon, 29 XTH genes were identified; most of these genes contain two conserved domains (Glyco_hydro_16 and XET_C domains). Based on neighbor-joining phylogenetic analysis, the CmXTHs were divided into four subfamilies, I/II, IIIA, and IIIB, which are distributed across nine chromosomes of melon. Collinearity analysis showed that the melon XTH genes have an evolutionary history consistent with three species: Arabidopsis, tomato, and cucumber. The promoter regions of the CmXTH genes contain numerous cis-acting elements, which are associated with plant growth, hormonal response, and stress responses. RNA-Seq analysis indicated that CmXTH genes exhibit different expression patterns under drought and salt stress treatments, suggesting that this gene family plays an important role under abiotic stress. This study provides a theoretical basis for further studies on the molecular function of XTH genes in melon.

Circulating tumor cells (CTCs) are detected in approximately 30% of metastatic non-small-cell lung cancer (NSCLC) cases using the CellSearch system, which relies on EpCAM immunomagnetic enrichment and Cytokeratin detection. This study evaluated the effectiveness of immunomagnetic enrichment targeting oncofetal chondroitin sulfate (ofCS) using recombinant VAR2CSA proteins (rVAR2) to improve the recovery of different NSCLC cell lines spiked into lysed blood samples. Four NSCLC cell lines—NCI-H1563, A549, NCI-H1792, and NCI-H661—were used to assess capture efficiency. The results demonstrated that the combined use of anti-EpCAM antibody and rVAR2 significantly enhanced the capture efficiency to an average of 88.2% compared with 40.6% when using only anti-EpCAM and 56.6% when using only rVAR2. These findings suggest that a dual-marker approach using anti-EpCAM and rVAR2 can provide a more robust and sensitive method for CTC enrichment in NSCLC, potentially leading to better diagnostic and prognostic outcomes.

Red and blue light have significant effects on plant growth; however, most of the current studies have focused on common horticultural crops such as cucumber and tomato, and there are fewer studies on how red and blue light affect the growth of melon seedlings. Therefore, in this study, we used melon (Cucumis melo L.) as the experimental material to investigate the effects of red and blue light ratios on the photomorphogenesis and photosynthesis of melon seedlings. Five red and blue light ratios were set at a fixed light intensity 200 μmol·m−2·s−1, including R:B = 1:9, R:B = 3:7, R:B = 1:1, R:B = 7:3, and R:B = 9:1. The results showed that with the increase in red light ratios, melon seedling height, stem diameter, total leaf area and stomatal pore size of melon seedlings increased, while the upper epidermis, palisade tissue, spongy tissue, leaf thickness, and stomatal density showed a decreasing trend. Under the R:B = 7:3 treatment, melon seedlings were optimized in all morphological indexes and had higher photosynthetic efficiency; these results indicated that the growth of melon seedlings could be regulated by adjusting the ratio of red to blue light, thus promoting the morphogenesis of melon seedlings.

Abstract With the development of distributed generation and power electronics technology, DC microgrids have made certain progress with their advantages of simple structure, economic efficiency, and easy control. Considering factors such as the geographical location of some residential areas, microgrids with fuel cells that can operate independently are also receiving increasing attention. In this paper, an online control method named virtual hydrogen consumption is proposed based on an independent DC microgrid which solves the problem of electro‐hydrogen conversion successfully. By solving the overall consumption function, the analytical solution is further obtained to realize the online control. Based on the results of RT‐LAB platform, the proposed method can maintain the output of fuel cell system in its high efficiency range with a lower volatility.

Porous Ti–6Al–4V alloys have been widely applied in industry, serving as structural or functional materials. However, the conventional method of producing porous Ti–6Al–4V by metal injection molding (MIM) with a space holder has several drawbacks, such as long debinding time, sintering furnace contamination, uncontrolled porosity and pore size, poor mechanical properties and process instability. This study presents a novel method of fabricating porous Ti–6Al–4V by MIM without space holder that can overcome these challenges. The porosity, average pore size, tensile strength, and compressive strength were studied by scanning electron microscope characterization, optical micrograph analysis, and mechanical testing. Flexible changes in porosity (17 %–74 %), pore size (14 μm–84 μm), and tensile strength (15 MPa–457 MPa) could be achieved by adjusting the powder size and sintering temperature. A higher porosity can be achieved as compared to the previous studies. The relationships among particle size, sintering temperature, porosity, and tensile strength are investigated by multiple regression models, which facilitate the tailored design of porous Ti–6Al–4V alloys for industrial applications and enhance the combination of experimental and theoretical methods in the design of porous alloys using MIM.

We present the dynamical equation model of the axially moving system, which is expressed through one partial differential equation (PDE) and two ordinary differential equations (ODEs) obtained using the extended Hamilton's principle. In the case of large acceleration/deceleration axially moving system with system parameters uncertainty and input saturation limitation, the combination of Lyapunov theory, S-curve acceleration and deceleration (Sc A/D) and adaptive control techniques adopts auxiliary systems to overcome the saturation limitations of the actuator, thus achieving the purpose of vibration suppression and improving the quality of vibration control. Sc A/D has better flexibility than that of constant speed to ensure the operator performance and diminish the force of impact by tempering the initial acceleration. The designed adaptive control law can avoid the control spillover effect and compensate the system parameters uncertainty. In practice, time-varying boundary interference and distributed disturbance exist in the system. The interference observer is used to track and eliminate the unknown disturbance of the system. The control strategy guarantees the stability of the closed-loop system and the uniform boundedness of all closed-loop states. The numerical simulation results test the effectiveness of the proposed control strategy.

Fermented soybeans are popular among many for their rich soy sauce-like aroma. However, the precise composition of this aroma remains elusive, with key aroma compounds unidentified. In this study, we screened the candidate genes ilvA and serA in BJ3-2 based on previous multi-omics data, and we constructed three mutant strains, BJ3-2-ΔserA, BJ3-2-ΔilvA, and BJ3-2-ΔserAΔilvA, using homologous recombination to fermented soybeans with varying intensities of soy sauce-like aroma. Our objective was to analyze samples that exhibited different aroma intensities resulting from the fermented soybeans of BJ3-2 and its mutant strains, thereby exploring the key flavor compounds influencing soy sauce-like aroma as well analyzing the effects of ilvA and serA on soy sauce-like aroma. We employed quantitative descriptive sensory analysis (QDA), gas chromatography–olfactometry–mass spectrometry (GC-O-MS), relative odor activity value analysis (rOAV), principal component analysis (PCA), orthogonal partial least squares-discriminant analysis (OPLS-DA), and partial least squares regression analysis (PLSR). QDA revealed the predominant soy sauce-like aroma profile of roasted and smoky aromas. GC-MS detected 99 volatile components, predominantly pyrazines and ketones, across the four samples, each showing varying concentrations. Based on rOAV (>1) and GC-O, 12 compounds emerged as primary contributors to soy sauce-like aroma. PCA and OPLS-DA were instrumental in discerning aroma differences among the samples, identifying five compounds with VIP > 1 as key marker compounds influencing soy sauce-like aroma intensity levels. Differential analyses of key aroma compounds indicated that the mutant strains of ilvA and serA affected soy sauce-like aroma mainly by affecting pyrazines. PLSR analysis indicated that roasted and smoky aromas were the two most important sensory attributes of soy sauce-like aroma, with pyrazines associated with roasted aroma and guaiacol associated with smoky aroma. In addition, substances positively correlated with the intensity of soy sauce-like aroma were verified by additional experiments. This study enhances our understanding of the characteristic flavor compounds in soy sauce-like aroma ferments, provides new perspectives for analyzing the molecular mechanisms of soy sauce-like aroma formation, and provides a theoretical framework for the targeted enhancement of soy sauce-like aroma in various foods.

Soybean flavor is considered to be essential for the aroma quality of fermented soybeans (FS) with Bacillus subtilis BJ3-2 (BJ3-2) at 37 °C. However, the key aroma compounds of the soybean flavor must be further elucidated. In this study, two candidate genes (sdaAA and katX) of BJ3-2 involved in the control of soybean flavor production were screened using prior multi-omics data. FS samples with BJ3-2, BJ3-2ΔsdaAA, BJ3-2ΔkatX, and BJ3-2ΔsdaAAΔkatX were analyzed by quantitative descriptive sensory analysis (QDA), gas chromatography–olfactometry–mass spectrometry (GC-O-MS), relative odor activity values (ROAV), and aroma addition experiments. The QDA revealed that the aroma profile of the soybean flavor in FS consisted of “sweaty”, “smoky”, “beany”, “roasted”, and “sweet” attributes. A total of 20 aroma-active compounds were detected, and 13 of them with ROAV > 1 were identified as key aroma compounds. Moreover, aroma addition experiments were conducted to further confirm the key aroma compounds of soybean flavor. Among them, 2-methylbutyric acid, 2,3,5-trimethylpyrazine, and guaiacol contributed higher aroma intensity values and ROAV, resulting in “sweaty”, “roasted”, and “smoky” attributes of soybean flavor in FS, respectively, while 1-octen-3-ol was associated with the “beany” attribute. These findings provide novel insights into the aroma attributes of soybean flavor in FS and a new strategy for revealing the key aroma compounds in fermented foods.

Light quality not only directly affects the photosynthesis of green plants but also plays an important role in regulating the development and movement of leaf stomata, which is one of the key links for plants to be able to carry out normal growth and photosynthesis. By sensing changes in the light environment, plants actively regulate the expansion pressure of defense cells to change stomatal morphology and regulate the rate of CO2 and water vapor exchange inside and outside the leaf. In this study, Cucumis melo was used as a test material to investigate the mitigation effect of different red, blue, and green light treatments on short-term drought and to analyze its drought-resistant mechanism through transcriptome and metabolome analysis, so as to provide theoretical references for the regulation of stomata in the light environment to improve the water use efficiency. The results of the experiment showed that after 9 days of drought treatment, increasing the percentage of green light in the light quality significantly increased the plant height and fresh weight of the treatment compared to the control (no green light added). The addition of green light resulted in a decrease in leaf stomatal conductance and a decrease in reactive oxygen species (ROS) content, malondialdehyde MDA content, and electrolyte osmolality in the leaves of melon seedlings. It indicated that the addition of green light promoted drought tolerance in melon seedlings. Transcriptome and metabolome measurements of the control group (CK) and the addition of green light treatment (T3) showed that the addition of green light treatment not only effectively regulated the synthesis of abscisic acid (ABA) but also significantly regulated the hormonal pathway in the hormones such as jasmonic acid (JA) and salicylic acid (SA). This study provides a new idea to improve plant drought resistance through light quality regulation.

Abstract In ferroelectrics, complex interactions among various degrees of freedom enable the condensation of topologically protected polarization textures. Known as ferroelectric solitons, these particle-like structures represent a new class of materials with promise for beyond-CMOS technologies due to their ultrafine size and sensitivity to external stimuli. Such polarization textures have scarcely been demonstrated in multiferroics. Here, we present evidence for ferroelectric solitons in (BiFeO3)/(SrTiO3) superlattices. High-resolution piezoresponse force microscopy and Cs-corrected high-angle annular dark-field scanning transmission electron microscopy reveal a zoo of topologies, and polarization displacement mapping of planar specimens reveals center-convergent/divergent topological defects as small as 3 nm. Phase-field simulations verify that some of these structures can be classed as bimerons with a topological charge of ±1, and first-principles-based effective Hamiltonian computations show that the coexistence of such structures can lead to non-integer topological charges, a first observation in a BiFeO3-based system. Our results open new opportunities in multiferroic topotronics.

This study aimed to enhance the use of male sterility in pepper to select superior hybrid generations. Transcriptomic and proteomic analyses of fertile line 1933A and nucleic male sterility line 1933B of Capsicum annuum L. were performed to identify male sterility-related proteins and genes. The phylogenetic tree, physical and chemical characteristics, gene structure characteristics, collinearity and expression characteristics of candidate genes were analyzed. The study identified 2,357 differentially expressed genes, of which 1,145 and 229 were enriched in the Gene Ontology and Kyoto Encyclopedia of Genes and Genomes databases, respectively. A total of 7,628 quantifiable proteins were identified and 29 important proteins and genes were identified. It is worth noting that the existence of CaPRX genes has been found in both proteomics and transcriptomics, and 3 CaPRX genes have been identified through association analysis. A total of 66 CaPRX genes have been identified at the genome level, which are divided into 13 subfamilies, all containing typical CaPRX gene conformal domains. It is unevenly distributed across 12 chromosomes (including the virtual chromosome Chr00). Salt stress and co-expression analysis show that male sterility genes are expressed to varying degrees, and multiple transcription factors are co-expressed with CaPRXs, suggesting that they are involved in the induction of pepper salt stress. The study findings provide a theoretical foundation for genetic breeding by identifying genes, metabolic pathways, and molecular mechanisms involved in male sterility in pepper.

Abstracts: Appropriate management of agricultural organic waste (AOW) presents a significant obstacle in the endeavor to attain sustainable agricultural development. The proper management of AOW is a necessity for sustainable agricultural development. This can be done skillfully by incorporating microbial agents in the composting procedure. In this study, we isolated relevant bacteria strains from tomato straw AOW, which demonstrated efficient degradation of lignocellulose without any antagonistic effects in them. These strains were then combined to create a composite microbial agent called Zyco Shield (ZS). The performance of ZS was compared with a commercially effective microorganism (EM) and a control CK. The results indicate that the ZS treatment significantly prolonged the elevated temperature phase of the tomato straw pile, showing considerable degradation of lignocellulosic material. This substantial degradation did not happen in the EM and CK treatments. Moreover, there was a temperature rise of 4–6 ℃ in 2 days of thermophilic phase, which was not the case in the EM and CK treatments. Furthermore, the inoculation of ZS substantially enhanced the degradation of organic waste derived from tomato straw. This method increased the nutrient content of the resulting compost and elevated the enzymatic activity of lignocellulose-degrading enzymes, while reducing the urease enzyme activity within the pile. The concentrations of NH4+-N and NO3--N showed increases of (2.13% and 47.51%), (14.81% and 32.17%) respectively, which is again very different from the results of the EM and CK treatments. To some extent, the alterations observed in the microbial community and the abundance of functional microorganisms provide indirect evidence supporting the fact that the addition of ZS microbial agent facilitates the composting process of tomato straw. Moreover, we confirmed the degradation process of tomato straw through X-ray diffraction, Fourier infrared spectroscopy, and by scanning electron microscopy to analyze the role of ZS microbial inoculum composting. Consequently, reinoculation compost strains improves agricultural waste composting efficiency and enhances product quality.

Abstract Solution growth of single-crystal ferroelectric oxide films has long been pursued for the low-cost development of high-performance electronic and optoelectronic devices. However, the established principles of vapor-phase epitaxy cannot be directly applied to solution epitaxy, as the interactions between the substrates and the grown materials in solution are quite different. Here, we report the successful epitaxy of single-domain ferroelectric oxide films on Nb-doped SrTiO3 single-crystal substrates by solution reaction at a low temperature of ~200 oC. The epitaxy is mainly driven by an electronic polarization screening effect at the interface between the substrates and the as-grown ferroelectric oxide films, which is realized by the electrons from the doped substrates. Atomic-level characterization reveals a nontrivial polarization gradient throughout the films in a long range up to ~500 nm because of a possible structural transition from the monoclinic phase to the tetragonal phase. This polarization gradient generates an extremely high photovoltaic short-circuit current density of ~2.153 mA/cm2 and open-circuit voltage of ~1.15 V under 375 nm light illumination with power intensity of 500 mW/cm2, corresponding to the highest photoresponsivity of ~4.306×10−3 A/W among all known ferroelectrics. Our results establish a general low-temperature solution route to produce single-crystal gradient films of ferroelectric oxides and thus open the avenue for their broad applications in self-powered photo-detectors, photovoltaic and optoelectronic devices.

Every year, a significant amount of pepper stalks are wasted due to low utilization. The ash produced from pepper stalks contains a significant amount of alkaline salts, which are food additives that can enhance the quality of noodles. Therefore, utilizing natural pepper straw ash to improve the quality of noodles shows promising development prospects. In this study, pepper straw ash leachate (PSAL) was extracted and added to noodles. The quality of the noodles gradually improved with the addition of PSAL, with the best effect observed at a concentration of 18% (PSAL mass/flour mass). This addition resulted in a 57.8% increase in noodle hardness, a 55.43% increase in chewiness, a 19.41% rise in water absorption rate, and a 13.28% increase in disulfide bond content. These alterations rendered the noodles more resilient during cooking, reducing their tendency to soften and thus enhancing chewiness and palatability. Incorporating PSAL also reduced cooking loss by 57.79%. Free sulfhydryl groups decreased by 5.1%, and scanning electron microscopy revealed a denser gluten network structure in the noodles, with more complete starch wrapping. This study significantly enhanced noodle quality and provided a new pathway for the application of pepper straw resources in the food industry.

Purpose: Emerging evidences have demonstrated that annexin A13 (ANXA13) is closely related to the occurrence and development of malignant tumors. However, the functions and underlying molecular mechanisms of ANXA13 in Clear cell renal cell carcinoma (ccRCC) have not been defined. Therefore, this study aimed to clarify the potential role of ANXA13 in regulating the proliferation, migration, invasion, cell cycle, and apoptosis of ccRCC cells. Patients and methods: The quantitative real-time PCR (qRT-PCR) and western blotting was performed for detecting the ANXA13 expression in ccRCC tissues at the mRNA and protein levels, respectively. The GEPIA2 databases were used to derive data for analyzing the ANXA13 expression in pan-cancer and ccRCC clinical features. Cell Counting and colony formation assays, as well as flow cytometry, were used to detect cell proliferation, apoptosis, or cell cycle. The wound healing assay was used to evaluate the migration ability of cells, and the Trans-well assay was conducted to determine the cell invasiveness. Results: ANXA13 was upregulated in ccRCC cells and human ccRCC tissues. Furthermore, siANXA13 inhibited ccRCC cell proliferation, migration, invasion and induced cell apoptosis. Conclusion: ANXA13 was upregulated in ccRCC. ANXA13 promotes tumorigenic traits of ccRCC cell lines in vitro. ANXA13 is a potential novel biomarker and a potential therapeutic target in ccRCC.

Inspired by the application of fungicide from natural products, a series of chalcone derivatives containing pyridazine were designed, synthesized, and evaluated for their antifungal activities against nine plant fungi and antibacterial activity against three plant bacteria. The antibacterial results revealed that B8 showed the best activity against Xanthomonas campestris pv. citri (Xac) with an EC50 value of 78.89 μg/mL, superior to Bismerthiazol (EC50 = 86.72 μg/mL). The antifungal bioactivity results showed that some of the compounds had good bioactivity against fungi, such as B4 showed the best bioactivity against Botrytis cinerea (BC) with an EC50 value of 8.91 μg/mL, which was better than the azoxystrobin (EC50 = 20.28 μg/mL). Compounds B4, D2 and D3 showed good biological activity against Rhizoctonia solani (RS), with an EC50 value of 18.10, 20.18 and 20.60 μg/mL, comparable to the azoxystrobin. Antifungal mechanism studies of BC by B4 suggest that B4 disrupts the cell membrane of the mycelium and thus inhibits the growth of the fungus. The above indicates that chalcone derivatives containing pyridazine have the potential to become fungicides.

Abstract Background Tetramethylpyrazine (TTMP) is a flavoring additive that significantly contributes to the formation of flavor compounds in soybean-based fermented foods. Over recent years, the application of TTMP in the food industry and medicine has been widely investigated. In addition, several methods for the industrial-scale production of TTMP, including chemical and biological synthesis, have been proposed. However, there have been few reports on the synthesis of TTMP through amino acid metabolic flux. In this study, we investigated genetic alterations of arginine metabolic flux in solid-state fermentation (SSF) of soybeans with Bacillus subtilis (B.subtilis) BJ3-2 to enhance the TTMP yield. Results SSF of soybeans with BJ3-2 exhibited a strong Chi-flavour (a special flavour of ammonia-containing smelly distinct from natto) at 37 °C and a prominent soy sauce-like aroma at 45 °C. Transcriptome sequencing and RT-qPCR verification showed that the rocF gene was highly expressed at 45 °C but not at 37 °C. Moreover, the fermented soybeans with BJ3-2ΔrocF (a rocF knockout strain in B. subtilis BJ3-2 were obtained by homologous recombination) at 45 °C for 72 h displayed a lighter color and a slightly decreased pH, while exhibiting a higher arginine content (increased by 14%) than that of BJ3-2. However, the ammonia content of fermented soybeans with BJ3-2ΔrocF was 43% lower than that of BJ3-2. Inversely, the NH4 + content in fermented soybeans with BJ3-2ΔrocF was increased by 28% (0.410 mg/kg). Notably, the TTMP content in fermented soybeans with BJ3-2ΔrocF and BJ3-2ΔrocF + Arg (treated with 0.05% arginine) were significantly increased by 8.6% (0.4617 mg/g) and 18.58% (0.504 mg/g) respectively than that of the BJ3-2. Conclusion The present study provides valuable information for understanding the underlying mechanism during the TTMP formation process through arginine metabolic flux.

With the continuous development of agriculture, Agricultural organic waste (AOW) has become the most abundant renewable energy on earth, and it is a hot spot of research in recent years to realize the recycling of AOW to achieve sustainable development of agricultural production. However, lignocellulose, which is difficult to degrade in AOW, greenhouse gas emissions, and pile pathogenic fungi and insect eggs are the biggest obstacles to its return to land use. In response to the above problems researchers promote organic waste recycling by pretreating AOW, controlling composting conditions and adding other substances to achieve green return of AOW to the field and promote the development of agricultural production. This review summarizes the ways of organic waste treatment, factors affecting composting and problems in composting by researchers in recent years, with a view to providing research ideas for future related studies.

Abstract Although soy sauce-like flavor and soybean flavor are two key contributors to the flavor of fermented foods, the key compounds of soy sauce-like flavor and soybean flavor and production mechanisms are still poorly understood and need further investigation. In the present study, we found that the Bacillus subtilis (B. subtilis) BJ3-2 strain has various metabolic properties at different temperatures, and the strain cultured at 37℃ increased the soybean flavor (a special flavor of ammonia-containing smelly distinct from natto) compared with culturing at 45℃ and 53℃. Interestingly, the strain cultured at 45℃ and 53℃ had a higher soy sauce-like flavor than that in 37℃. Moreover, a comparative transcriptome analysis of the strain cultured at 37℃, 45℃, and 53℃ showed transcriptional changes related to secondary metabolites and ABC transporters, which is critical for the amino acid transport and metabolism in B. subtilis. Meanwhile, proteomics and metabolomics profiling showed a marked change in amino acids transport and metabolism. In addition, the metabolic analysis revealed a significant metabolic difference (including sulfur metabolism, glutathione metabolism, nicotinate and nicotinamide metabolism, cysteine and methionine metabolism, and pyrimidine metabolism) in the strain cultured at 45℃ and 53℃ compared to 37℃. To sum, this study used the multi-omics profiling tool to investigate the fermentative strains B. subtilis BJ3-2, thus providing a deeper insight into the mechanism of the formation of soy sauce-like flavor and soybean flavor compounds.

Abstract Background The accuracy of CT and tumour markers in screening lung cancer needs to be improved. Computer-aided diagnosis has been reported to effectively improve the diagnostic accuracy of imaging data, and recent studies have shown that circulating genetically abnormal cell (CAC) has the potential to become a novel marker of lung cancer. The purpose of this research is explore new ways of lung cancer screening. Methods From May 2020 to April 2021, patients with pulmonary nodules who had received CAC examination within one week before surgery or biopsy at First Affiliated Hospital of Zhengzhou University were enrolled. CAC counts, CT scan images, serum tumour marker (CEA, CYFRA21–1, NSE) levels and demographic characteristics of the patients were collected for analysis. CT were uploaded to the Pulmonary Nodules Artificial Intelligence Diagnostic System (PNAIDS) to assess the malignancy probability of nodules. We compared diagnosis based on PNAIDS, CAC, Mayo Clinic Model, tumour markers alone and their combination. The combination models were built through logistic regression, and was compared through the area under (AUC) the ROC curve. Results A total of 93 of 111 patients were included. The AUC of PNAIDS was 0.696, which increased to 0.847 when combined with CAC. The sensitivity (SE), specificity (SP), and positive (PPV) and negative (NPV) predictive values of the combined model were 61.0%, 94.1%, 94.7% and 58.2%, respectively. In addition, we evaluated the diagnostic value of CAC, which showed an AUC of 0.779, an SE of 76.3%, an SP of 64.7%, a PPV of 78.9%, and an NPV of 61.1%, higher than those of any single serum tumour marker and Mayo Clinic Model. The combination of PNAIDS and CAC exhibited significantly higher AUC values than the PNAIDS (P = 0.009) or the CAC (P = 0.047) indicator alone. However, including additional tumour markers did not significantly alter the performance of CAC and PNAIDS. Conclusions CAC had a higher diagnostic value than traditional tumour markers in early-stage lung cancer and a supportive value for PNAIDS in the diagnosis of cancer based on lung nodules. The results of this study offer a new mode of screening for early-stage lung cancer using lung nodules.

In order to investigate whether an end-of-day (EOD) addition of a single light quality could help alleviate high-light stress in a cucumber, cucumber seedlings were subjected to a 9 d period of high-light stress (light intensity was 1300 ± 50 μmol·m−2·s−1) when they were growing to 3 leaves and 1 heart, while the red light (R), blue light (B), green light (G), far-red light (FR), and ultraviolet A (UVA) light were added in the end-of-day period. The present study was conducted to measure antioxidants, chlorophyll content, and its synthetic degradative enzymes and chlorophyll a fluorescence in response to the degree of stress in cucumber seedlings. The experimental results demonstrated that the addition of blue light, UVA light, and green light significantly decreased the SOD and POD activities in the middle of the treatment (6th day) compared to the dark (D) treatment and improved the absorption performance of the PSI reaction centre of the cucumber seedling leaves to a certain extent (PIABS), but the PSII capacity capture ability (TRo/RC) of the three treatments decreased compared to the D treatment. The MDA content of all the treatments had a significant decrease compared to that of the D treatment. The MDA content of all the treatments was significantly lower than that of D, and its FV/FM was increased to different degrees; the chlorophyll degrading enzyme PPH activity was significantly lower than that of the D treatment when a single light quality was added at the EOD period on the 9th d of treatment. In conclusion, cucumber seedlings subjected to short-term high-light stress can be added during the EOD period with a low-light intensity of a single R, G, B, or UVA light.

BackgroundThere is growing evidence demonstrating that the gut microbiota plays a crucial role in multiple endocrine disorders, including diabetic cardiomyopathy (DCM). Research shows that the Chinese herb reduces disease occurrence by regulating gut microbiota. Zuogui Jiangtang Shuxin formula (ZGJTSXF), a Chinese medicinal formula, has been clinically used for treatment of DCM for many years. However, there is still no clear understanding of how ZGJTSXF treatment contributes to the prevention and treatment of DCM through its interaction with gut microbiota and metabolism.MethodsIn this study, mice models of DCM were established, and ZGJTSXF’s therapeutic effects were assessed. Specifically, serum glycolipid, echocardiography, histological staining, myocardial apoptosis rate were assessed. Using 16s rRNA sequencing and high-performance liquid chromatography-tandem mass spectrometry (HPLC-MS/MS), we determined the impact of ZGJTSXF on the structure of gut microbiota and content of its metabolite TMAO. The mechanism of ZGJTSXF action on DCM was analyzed using quantitative real-time PCR and western blots.ResultsWe found that ZGJTSXF significantly ameliorated DCM mice by modulating gut-heart axis: ZGJTSXF administration improved glycolipid levels, heart function, cardiac morphological changes, inhibited cardiomyocytes apoptosis, and regulate the gut microbiota in DCM mice. Specifically, ZGJTSXF treatment reverse the significant changes in the abundance of certain genera closely related to DCM phenotype, including Lactobacillus, Alloprevotella and Alistipes. Furthermore, ZGJTSXF alleviated DCM in mice by blunting TMAO/PERK/FoxO1 signaling pathway genes and proteins.ConclusionZGJTSXF administration could ameliorate DCM mice by remodeling gut microbiota structure, reducing serum TMAO generation and suppressing TMAO/PERK/FoxO1 signaling pathway.

Piezoelectric materials that can effectively convert natural mechanical energy into electrical energy without time and space constraints have been widely applied for energy harvesting and conversion. The piezocomposites with high piezoelectricity and flexibility have shown great promise for renewable electric energy generation that can power implantable and wearable electronics. This minireview aims to summarize the recent progress of the piezocomposites with different composite structures, as well as the role of the theoretical understandings and designs in the development of new piezoelectric nanogenerator materials. Thereinto, the most common composite structural types (0-3, 1-3, and 3-3) have been discussed systematically. Several strategies for high output performance of piezocomposites are also proposed on the basis of current experimental and simulation results. Finally, the review concludes with perspectives on the future design of flexible piezoelectric nanocomposites for energy harvesters.

Non-alcoholic fatty liver disease (NAFLD) pathogenesis is affected by dysbiosis of the gut microbiome and the metabolites it generates. Therefore, restoring the equilibrium between the gut microbiome and the generated metabolites may have therapeutic potential for the syndrome. Zuogui Jiangtang Qinggan Fang (ZGJTQGF) is a Chinese herbal formulation used clinically to treat type 2 diabetic mellitus (T2DM) and fatty liver disease. However, its pharmacological mechanisms have not been well characterized. This work aimed to evaluate the hepatoprotective mechanism of ZGJTQGF in T2DM with NAFLD mice by incorporating gut microbiota, short-chain fatty acids（SCFAs）, and metabolomic analysis, and then to provide strong support for clinical treatment of T2DM with NAFLD. The sequencing of 16 S rRNA revealed that ZGJTQGF therapy modified the composition and abundance of the gut microbiome, raised the level of SCFAs, and restored the intestinal mucosal barrier. The non-targeted metabolomic analysis of liver tissues identified 212 compounds, of which108 were differentially expressed between the HFD and ZGJTQGF groups. Moreover, L-glutamic acid, L-Phenylalanine, Glycine, Taurine, Deoxycholic acid, and citric acid levels were also considerably altered by ZGJTQGF. Our findings suggest that ZGJTQGF ameliorates HFD-induced hepatic steatosis by modulating the gut microbiota composition and its metabolites and boosting the levels of SCFAs. More notably, ZGJTQGF may be a promising medication for preventing and treating NAFLD.

Many pepper cultivars can be raised under artificial lighting in a plant factory. An easily measured parameter is needed to fast predict fruit loading in pepper cultivars. In this study, four pepper cultivars with contrasting manners in growth and fruiting were cultured under three light-emitting diode (LED) spectra in comparison with a sunlight control. It was found that the red-light spectrum (71.7% red-, 13.7% green-, 14.6% blue-lights) increased over 40% of dry mass in fruits, while the green-light spectrum (26.2% red-, 56.4% green-, 17.4% blue-lights) induced no fruiting compared to the control. Only two cultivars responded by fine root morphology, which was characterized as smaller surface-area and fewer tip-number in the blue-light spectrum (7.8% red-, 33.7% green-, 48.5% blue-lights) than in red LED light. Tip-number showed a negative correlation with fruit dry-mass in three cultivars, while fine root diameter increased with dry mass in fruits. In conclusion, fine root tip-number can be used as a predictor of fruit dry-mass in pepper cultivars high in fruit quality or yield. The red-colour light was recommended for raising pepper cultivars in a plant factory with the purpose of greater fruit productivity.

Summary: Phase-field simulation is a powerful tool for understanding lithium metal electrodeposition. This protocol outlines the process of numerically solving the phase-field equations using the MOOSE framework. Here, we describe steps to obtain the spatiotemporal distribution of major physical characteristics such as phase-field, ion concentration, overpotential, and driving force. Such an approach may help to reveal the underlying physics and kinetics of dendrite growth, while also providing design principles for suppressing lithium dendrites.For complete details on the use and execution of this protocol, please refer to Hong and Viswanathan (2018). : Publisher’s note: Undertaking any experimental protocol requires adherence to local institutional guidelines for laboratory safety and ethics.

Abstract Sonodynamic therapy (SDT), presenting spatial and temporal control of ROS generation triggered by ultrasound field, has attracted considerable attention in tumor treatment. However, its therapeutic efficacy is severely hindered by the intrinsic hypoxia of solid tumor and the lack of smart design in material band structure. Here in study, fine α-Fe2O3 nanoparticles armored with Pt nanocrystals (α-Fe2O3@Pt) was investigated as an alternative SDT agent with ingenious bandgap and structural design. The Schottky barrier, due to its unique heterostructure, suppresses the recombination of sono-induced electrons and holes, enabling superior ROS generation. More importantly, the composite nanoparticles may effectively trigger a reoxygenation phenomenon to supply sufficient content of oxygen, favoring the ROS induction under the hypoxic condition and its extra role played for ultrasound imaging. In consequence, α-Fe2O3@Pt appears to enable effective tumor inhibition with imaging guidance, both in vitro and in vivo. This study has therefore demonstrated a highly potential platform for ultrasound-driven tumor theranostic, which may spark a series of further explorations in therapeutic systems with more rational material design. Graphical Abstract

BackgroundIntrahepatic biliary cystadenoma (IBC) is a rare benign cystic tumor of the liver. So far, it has not been comprehensively understood, which causes incorrect diagnosis, treatment confusion, and even inappropriate treatment. Here, we reviewed clinical data of IBC patients in our center, shared our experiences and lessons learned, and improved the level of diagnosis and treatment.MethodsThe clinical data of 10 patients with pathologically diagnosed IBC, admitted to the Department of Hepatobiliary Surgery of the Affiliated Hospital of Guangdong Medical University from January, 2007, to January, 2022 were retrospectively analyzed.Results10 patients underwent surgery and were discharged successfully. Cyst morphology: multiple cysts: 6 cases (6/10), monocular cyst: four cases(4/10). Six patients (6/10) were diagnosed as IBC preoperatively and received hepatectomy. Four patients with monocular cyst IBC underwent intraoperative frozen section examination, except one case showed IBC; the rest were misdiagnosed as simple liver cyst. In three misdiagnosed patients, one underwent open left hepatectomy seven days after the initial operation. The other patient refused to undergo reoperation and required follow-up observation. The last patient could not tolerate hepatectomy due to insufficient residual liver volume and chose follow-up observationConclusionFor IBC, especially monocular IBC, it is easy to be misdiagnosed as simple hepatic cyst, which brings great confusion to clinical treatment. We propose strengthening communication with pathologists to deepen understanding of IBC. Attention should be paid to the cyst wall’s shape and the cyst fluid’s properties during the operation to avoid the missed diagnosis, misdiagnosis, or even improper operation. For suspicious cases, directly choose hepatectomy to avoid reoperation after thoroughly evaluating the patient’s condition.

Light can regulate leaf stomatal development and movement, but the effects of different red-to-blue light mass ratios on leaf stomatal morphology and openness are not fully understood. In this trial, five different red-to-blue light (R:B) ratio treatments were used to study the changes in morphology, photosynthesis, and stomatal-related indexes of cucumber seedlings under fixed light intensity (200 μmol·m−2·s−1). The results showed that the thickness of spongy tissue and stomatal size (SZ) of cucumber seedling leaves decreased, and the photosynthetic potential, stomatal density (SD), maximum stomatal conductance and stomatal responsiveness increased with decreasing R:B content. The experimental results showed that when R:B = is 1:9, cucumber seedlings had the greatest stomatal density and the fastest response rate, and the stomatal opening rate was accelerated with the increase in the proportion of blue light; when R:B = is 3:7, the stomatal conductance was the greatest and the net photosynthetic rate was the highest. This trial provides some implications for changing plant light quality and thus affecting stomatal development and movement.

Zao pepper is a fermented type with a distinct flavor, and Zao pepper-based dishes are popular. However, in spontaneous fermentation (SF), the adverse microorganisms of Zao pepper are uncontrollable and alter the product quality. Lactic acid bacteria (LAB) inhibit the growth of harmful bacteria and endow a sour taste in SF. Therefore, the purpose of this study was to isolate autochthonic LAB from SF and through inoculated fermentation (IF) to improve Zao pepper quality. Four LAB strains were selected for probiotic experiments by sensory evaluation based on the flavor of Zao pepper in vitro. Intriguingly, Lactobacillus plantarum 5-1 showed superior safety and probiotics, with the fastest growth and acid-production rate. Moreover, the nitrite reductase viability of L. plantarum 5-1 reached 4.73 μmol/mL·h. The use of IF via L. plantarum 5-1 increased the total acid content 2-fold and reduced the nitrite content by 0.36-fold compared to SF in Zao pepper. In addition, IF improved the color and texture of Zao pepper. These results indicate that the autochthonic L. plantarum 5-1 improved the quality of Zao pepper, providing a new strategy for enhancing the stability of fermented pepper.

BackgroundPneumonia is an infection of the lungs that is characterized by high morbidity and mortality. The use of machine learning systems to detect respiratory diseases via non-invasive measures such as physical and laboratory parameters is gaining momentum and has been proposed to decrease diagnostic uncertainty associated with bacterial pneumonia. Herein, this study conducted several experiments using eight machine learning models to predict pneumonia based on biomarkers, laboratory parameters, and physical features.MethodsWe perform machine-learning analysis on 535 different patients, each with 45 features. Data normalization to rescale all real-valued features was performed. Since it is a binary problem, we categorized each patient into one class at a time. We designed three experiments to evaluate the models: (1) feature selection techniques to select appropriate features for the models, (2) experiments on the imbalanced original dataset, and (3) experiments on the SMOTE data. We then compared eight machine learning models to evaluate their effectiveness in predicting pneumoniaResultsBiomarkers such as C-reactive protein and procalcitonin demonstrated the most significant discriminating power. Ensemble machine learning models such as RF (accuracy = 92.0%, precision = 91.3%, recall = 96.0%, f1-Score = 93.6%) and XGBoost (accuracy = 90.8%, precision = 92.6%, recall = 92.3%, f1-score = 92.4%) achieved the highest performance accuracy on the original dataset with AUCs of 0.96 and 0.97, respectively. On the SMOTE dataset, RF and XGBoost achieved the highest prediction results with f1-scores of 92.0 and 91.2%, respectively. Also, AUC of 0.97 was achieved for both RF and XGBoost models.ConclusionsOur models showed that in the diagnosis of pneumonia, individual clinical history, laboratory indicators, and symptoms do not have adequate discriminatory power. We can also conclude that the ensemble ML models performed better in this study.

As a condiment with extensive nutritional value, chili is easy to be contaminated by Aspergillus flavus (A. flavus) during field, transportation, and storage. This study aimed to solve the contamination of dried red chili caused by A. flavus by inhibiting the growth of A. flavus and detoxifying aflatoxin B1 (AFB1). In this study, Bacillus subtilis E11 (B. subtilis) screened from 63 candidate antagonistic bacteria exhibited the strongest antifungal ability, which could not only inhibit 64.27% of A. flavus but could also remove 81.34% of AFB1 at 24 h. Notably, scanning electron microscopy (SEM) showed that B. subtilis E11 cells could resist a higher concentration of AFB1, and the fermentation supernatant of B. subtilis E11 could deform the mycelia of A. flavus. After 10 days of coculture with B. subtilis E11 on dried red chili inoculated with A. flavus, the mycelia of A. flavus were almost completely inhibited, and the yield of AFB1 was significantly reduced. Our study first concentrated on the use of B. subtilis as a biocontrol agent for dried red chili, which could not only enrich the resources of microbial strains for controlling A. flavus but also could provide theoretical guidance to prolong the shelf life of dried red chili.

In this study, a target tracking algorithm based on the flower pollination algorithm (FPA) is proposed. This method solves the problem of robust visual target tracking in different complex tracking scenes with the good global and local optimisation ability of the FPA. Meanwhile, with the aim of solving the problem of invalid background feature interference and the loss of effective features caused by the fixed scale of tracking frame in traditional tracking methods, a scale adaptive adjustment model of tracking frame is proposed. Considering that the FPA has good global and local optimization ability at simultaneously, the position update equation of the FPA is introduced as the main optimization method of target tracking. In addition, considering that the traditional FPA is similar to classical swarm intelligence algorithm (such as the particle swarm optimization algorithm), it also faces the problems of a high probability of falling into local extrema, a low efficiency of late convergence speed and a high probability of early maturity. Therefore, this work proposes the GTFPA, an advanced FPA based on the gravitational search algorithm (GSA) and mutation mechanism via a trigonometric function. We qualitatively, quantitatively and statistically compare the proposed method with other classical general tracking methods through two datasets, OTB2015 and VOT2018, which contain hundreds of video sequences and more than ten tracking scenes and can effectively test the success rate, accuracy and stability of the trackers. The results of a large number of tracking experiments in a variety of complex tracking scenarios prove that the proposed GTFPA tracker performs well with regards to efficiency, accuracy and robustness.

Abstract Background Cervicocerebral artery dissection is an important cause of ischemic stroke in young and middle-aged individuals. However, very few studies have compared the differential features between internal carotid artery dissection (ICAD) and vertebral artery dissection (VAD), including both cervical and intracranial artery dissections. We conducted a study to investigate the predisposing factors and radiological features in patients with ICAD or VAD. Methods All cases diagnosed with cervicocerebral artery dissection, ICAD, or VAD were identified through a medical records database, between January 2010 and January 2020. Baseline characteristics, predisposing factors, and radiological features of ICAD versus VAD were compared. Results A total of 140 patients with cervicocerebral artery dissection were included in the study, including 84 patients in the ICAD group and 56 in the VAD group. The mean age of patients in the ICAD and VAD groups was 43.37 ± 14.01 and 41.00 ± 12.98 years old, respectively. Patients with ICAD were more likely to be men compared with VAD (85.71% vs. 67.86%, p = 0.012). The frequency of hypertension, diabetes, smoking, drinking, and cervical trauma did not differ between ICAD and VAD. Dissections of ICAD were more frequently at the extracranial portions of the artery compared with those of VAD (70.24% vs. 44.64%, p = 0.003). In contrast, dissections of VAD were more common in the intracranial artery (55.36% vs. 29.76%, p = 0.003). Radiologically, double lumen (36.90% vs. 19.64%, p = 0.029) and intimal flap (11.90% vs. 1.79%, p = 0.029) were more frequently observed in ICAD than in VAD, and dissecting aneurysms were less frequent (13.10% vs. 26.79%, p = 0.041). Conclusions The distributions of cervical and intracranial artery dissections were different between ICAD and VAD. The frequencies of radiological features detected in patients with ICAD and VAD also differed.

Abstract Piezoelectric nanocomposites with oxide fillers in a polymer matrix combine the merit of high piezoelectric response of the oxides and flexibility as well as biocompatibility of the polymers. Understanding the role of the choice of materials and the filler‐matrix architecture is critical to achieving desired functionality of a composite towards applications in flexible electronics and energy harvest devices. Herein, a high‐throughput phase‐field simulation is conducted to systematically reveal the influence of morphology and spatial orientation of an oxide filler on the piezoelectric, mechanical, and dielectric properties of the piezoelectric nanocomposites. It is discovered that with a constant filler volume fraction, a composite composed of vertical pillars exhibits superior piezoelectric response and electromechanical coupling coefficient as compared to the other geometric configurations. An analytical regression is established from a linear regression‐based machine learning model, which can be employed to predict the performance of nanocomposites filled with oxides with a given set of piezoelectric coefficient, dielectric permittivity, and stiffness. This work not only sheds light on the fundamental mechanism of piezoelectric nanocomposites, but also offers a promising material design strategy for developing high‐performance polymer/inorganic oxide composite‐based wearable electronics.

Background and ObjectivesCraniocervical artery dissection (CAD) is the most common cause of ischemic stroke in young adults. The etiologies of CAD can be classified into three types, such as spontaneous (sCAD), minor traumatic (mtCAD), and genetic origin. Recent studies indicated that clinical presentations and imaging features could guide management and inform prognosis. This retrospective analysis sought to compare the clinical and imaging features of sCAD vs. mtCAD in providing evidence-based advice on medical treatment, functional rehabilitation, secondary stroke prevention, and prognosis, ultimately formulating clinical guidelines in managing CAD.MethodsIn total, 148 patients with CAD were identified from the medical records database and subdivided into sCAD and mtCAD based on the clinical presentations and imaging features. A retrospective comparative analysis was performed according to their clinical presentations and imaging features.ResultsPatients with mtCAD are significantly younger than sCAD with 120 cases of sCAD average aged 43.61 ± 12.75, while 28 cases of mtCAD average aged 35.68 ± 14.54. Patients with mtCAD had more cases of neck pain compared to sCAD. Patients with mtCAD had more cases of CAD at extracranial locations compared to sCAD. Patients with mtCAD had more cases of multiple site dissection compared to sCAD. Double lumen and intramural haematoma are the most common imaging findings with mtCAD patients having statistical significantly more cases of intramural haematoma and long tapering stenosis.ConclusionPatients with mtCAD were presented at a much younger age with symptoms of neck pain compared to sCAD. Patients with mtCAD predominantly presented at extracranial sites with more prominent features of multiple site dissection, intramural haematoma, and long tapering stenosis. These clinical and imaging features can translate into clinical practice guidelines for patients with CAD to improve the optimal functional outcome and reduce both morbidity and mortality.