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This paper proposes an optimization method for electric vehicle charging station locations considering dynamic charging demand. Firstly, the driving characteristics and charging characteristics of the electric vehicle are obtained based on the driving trajectory of the electric vehicle, and the charging demand is predicted using a Monte Carlo simulation. Then a mathematical model with the goal of minimizing the overall cost is constructed, and the impact on carbon emissions is considered in the model. In order to better solve the location model, an improved whale optimization algorithm based on a hybrid strategy is proposed. Finally, the location problem of Shenzhen electric taxi charging stations is analyzed as an example. The results show that when the number of charging stations is set to 19, the comprehensive cost is the smallest and the energy saving and emission reduction effect is good. The improved whale optimization algorithm also has higher solution accuracy and convergence speed than other classical algorithms.

This study provides a rapid and low-energy method for the preparation of nanocomposite hydrogels with excellent mechanical properties and electrical conductivity.

Compared with the traditional lattice structure, the triply periodic minimal surface (TPMS) structure can avoid stress concentration effectively. Here, it is promising in the fields of lightweight and energy absorption. However, the number of structural parameters and mechanical properties of the TPMS structure is plentiful, and the relationship between them is unclassified. In this paper, for the first time, a unified mathematical model was proposed to establish the relationship between TPMS structural design parameters and mechanical properties. Fifteen primitive models were designed by changing the structural parameters (level-set value C and thickness T) and manufacturing by selective laser melting. The geometric defects and surface quality of the structures were explored by optical microscope and scanning electron microscopy (SEM). The mechanical properties were investigated by quasi-static compression test and finite element simulation. The influence of building direction on structural mechanical behavior (failure mode, stress-strain curve) was studied. The real mechanical properties (Young’s modulus and plateau stress) of the structure could be predicted according to different C and T combinations. Finally, the energy absorption characteristics were explored. The results showed that when the C value is 0.6 in the range of 0–0.6, the energy absorption performance of the structure is at the maximum level.

Background The recently developed biomimetic strategy is one of the mostly effective strategies for improving the theranostic efficacy of diverse nanomedicines, because nanoparticles coated with cell membranes can disguise as “self”, evade the surveillance of the immune system, and accumulate to the tumor sites actively. Results Herein, we utilized mesenchymal stem cell memabranes (MSCs) to coat polymethacrylic acid (PMAA) nanoparticles loaded with Fe(III) and cypate—an derivative of indocyanine green to fabricate Cyp-PMAA-Fe@MSCs, which featured high stability, desirable tumor-accumulation and intriguing photothermal conversion efficiency both in vitro and in vivo for the treatment of lung cancer. After intravenous administration of Cyp-PMAA-Fe@MSCs and Cyp-PMAA-Fe@RBCs (RBCs, red blood cell membranes) separately into tumor-bearing mice, the fluorescence signal in the MSCs group was 21% stronger than that in the RBCs group at the tumor sites in an in vivo fluorescence imaging system. Correspondingly, the T1-weighted magnetic resonance imaging (MRI) signal at the tumor site decreased 30% after intravenous injection of Cyp-PMAA-Fe@MSCs. Importantly, the constructed Cyp-PMAA-Fe@MSCs exhibited strong photothermal hyperthermia effect both in vitro and in vivo when exposed to 808 nm laser irradiation, thus it could be used for photothermal therapy. Furthermore, tumors on mice treated with phototermal therapy and radiotherapy shrank 32% more than those treated with only radiotherapy. Conclusions These results proved that Cyp-PMAA-Fe@MSCs could realize fluorescence/MRI bimodal imaging, while be used in phototermal-therapy-enhanced radiotherapy, providing desirable nanoplatforms for tumor diagnosis and precise treatment of non-small cell lung cancer.

In this study, a simple and eco-friendly method was proposed to efficiently prepare nanocomposite hydrogels with excellent mechanical properties and satisfactory pH response behaviour by frontal polymerization (FP) of DEM in close to 4 minutes. Acrylamide (AM) and choline chloride (ChCl) were used as raw materials to synthesize deep eutectic monomers (DEMs). Nitrogen-doped carbon nanotubes were dispersed in DEMs as fillers, and poly(acrylamide)/nitrogen-doped carbon nanotube (PAM/N-CNT) nanocomposite hydrogels were prepared by FP. The non-covalent interactions between PAM hydrogels and N-CNTs was verified by Fourier infrared spectroscopy. The mechanical properties of PAM/N-CNT nanocomposite hydrogels were investigated, as well as the swelling and pH response properties. The results showed that the compressive strength of PAM hydrogels was significantly enhanced by the addition of N-CNTs due to the hydrophobic interaction of N-CNTs, which also causes sensitive response properties of the PAM hydrogels in acid solution.

Implantable batteries are critical for operation of most implanted electronic devices that are revolutionizing the healthcare field nowadays. However, the employed electrolytes for existing implanted batteries are mainly based on toxic organic solutions, which poses serious potential safety problems when they are implanted into the human body. Furthermore, current implanted batteries are generally bulky and rigid with stiff encapsulations, so they mechanically mismatch soft biological tissues with potential foreign body responses. Here, we present a biocompatible and rechargeable fiber battery that used carbon nanotube hybrid fibers as electrodes and did not require encapsulation to realize much higher softness. The fiber battery can be thus injected into various regions of the body through a mini-invasive syringe and delivered a power density of 78.9 mW cm−3in vivo, which is sufficient to drive various implanted electronic devices. The injectable fiber battery formed stable interfaces with tissues and showed high performances in the brain, heart and subcutis. As a demonstration, it was injected into the subcutis of a mouse to power an implanted sensor for respiration monitoring.

The loading capacity in the axial direction of a bolted thin steel plate was investigated. A refined numerical model of bolt was first constructed and then validated using existing experiment results. Parametrical analysis was performed to reveal the influences of geometric parameters, including the effective depth of the cap nut, the yield strength of the steel plate, the preload of the bolt, and shear force, on the ultimate loading capacity. Then, an analytical method was proposed to predict the ultimate load of the bolted thin steel plate. Results derived using the numerical and analytical methods were compared and the results indicated that the analytical method can accurately predict the pull-through capacity of bolted thin steel plates. The work reported in this paper can provide a simplified calculation method for the loading capacity in the axial direction of a bolt.

Antibody-drug conjugates have shown great promise in active targeting for cancer therapy. The existing chemical techniques for antibody conjugation generally lack efficiency or universality. In this article, a site-specific antibody conjugation was developed by using a mild reaction between a benzoboroxole (BB) functionality and cis-diol moiety of sugar units in the antibody fragment crystallizable region under neutral pH conditions. A BB/PEG/ICG-grafted poly(aspartic acid) comb-like functional polymer was first synthesized and conjugated with transferrin (Tf) to form a transferrin-polymer-drug conjugate [Tf-P(BB)], which showed 120% increase in HepG2 hepatoma (Tf receptor overexpression) cell uptake compared to a nontargeting protein-polymer-drug conjugate [HRP-P(BB)]. The universality of this method was further demonstrated by the enhanced uptake of trastuzumab (anti-Her2 antibody)-polymer-drug conjugates in MCF-7 (295%) and MDA-MB-435S (66.4%) (Her2 positive) cells. The positive charge of the linker had great influence on the targeting ability of the antibody-polymer-drug conjugates. The in vivo studies demonstrated the distinct targeting ability of Tf-P(BB) in the HepG2 xenograft tumor, and the tumor accumulation of the Tf-P(BB) testing group increased by 92% with respect to the control group [HRP-P(BB)]. More significantly, the HepG2 cell uptake amount of the antibody-oriented conjugate [Tf-P'(BB)] was 2.4-fold higher than that of the controlled group [Tf-P'(Hex)]. On the basis of this facile site-specific conjugation method, the conjugates are able to change the antibody species easily against various cancers, while maintaining the antibody integrity and targeting ability.

Fast growth is one of the most important breeding goals for all economic species such as the Pacific oyster (Crassostrea gigas), an aquaculture mollusk with top global production. Although the genetic basis and molecular mechanisms of growth-related traits have been widely investigated in the oyster, the role of DNA methylation involved in growth regulation remains largely unexplored. In this study, we performed a comparative DNA methylome analysis of two selectively bred C. gigas strains with contrasted phenotypes in growth and shell color based on whole-genome bisulfite sequencing (WGBS). Genome-wide profiling of DNA methylation at the single-base resolution revealed that DNA methylations were widely spread across the genome with obvious hotspots, coinciding with the distribution of genes and repetitive elements. Higher methylation levels were observed within genic regions compared with intergenic and promoter regions. Comparative analysis of DNA methylation allowed the identification of 339,604 differentially methylated CpG sites (DMCs) clustering in 27,600 differentially methylated regions (DMRs). Functional annotation analysis identified 11,033 genes from DMRs which were enriched in biological processes including cytoskeleton system, cell cycle, signal transduction, and protein biosynthesis. Integrative analysis of methylome and transcriptome profiles revealed a positive correlation between gene expression and DNA methylation within gene-body regions. Protein-protein interaction (PPI) analysis of differentially expressed and methylated genes allowed for the identification of integrin beta-6 (homolog of human ITGB3) as a hub modulator of the PI3K/Akt signaling pathway that was involved in various growth-related processes. This work provided insights into epigenetic regulation of growth in oysters and will be valuable resources for studying DNA methylation in invertebrates.

This paper focuses on the noise reduction of the cylindrical structure at low frequency (130 Hz-180 Hz). The low-frequency noise response spectrum in the cylindrical cavity is obtained by using the Helmholtz resonator (HR) to reduce the noise peak amplitude. The acoustic simulation software Virtual.Lab is used to establish the finite element model of the cylindrical shell with HR, and to obtain the low-frequency acoustic response in the cylindrical cavity. The simulation model is validated by the experimental results. Then, the influence of installation position, the number of installed resonators and the resonators with different resonance frequencies in the cylindrical cavity are discussed. The results indicate that both the noise reduction band and peak amplitude are increased by installing the HR's on the cylinder shell. The noise reduction of the cylinder shell with HR installed on the upper position is larger than other situations. As the number of resonators increased, the frequency range of the noise reduction in the cylindrical cavity gradually increases, and the noise reduction of the cylinder cavity increases first and then decreases.

Mechanical mismatches between implanted electronics and biological tissues can lead to inaccurate readings and long-term tissue damage. Here, we show that functionalized multi-walled carbon nanotubes twisted into helical fibre bundles that mimic the hierarchical structure of muscle can monitor multiple disease biomarkers in vivo. The flexible fibre bundles are injectable, have a low bending stiffness and display ultralow stress under compression. As proof-of-concept evidence of the sensing capabilities of these fibre bundles, we show that the fibre bundles enable the spatially resolved and real-time monitoring of H2O2 when implanted in tumours in mice, and that they can be integrated with a wireless transmission system on an adhesive skin patch to monitor calcium ions and glucose in the venous blood of cats for 28 d. The versatility of the helical fibre bundles as chemically functionalized electrochemical sensors makes them suitable for multiple sensing applications in biomedicine and healthcare. Functionalized multi-walled carbon nanotubes twisted into helical fibre bundles that mimic the hierarchical structure of muscle can be used for the long-term monitoring of multiple disease biomarkers in vivo.

This paper is devoted to the location selection method for electric vehicle charging stations utilizing the improved immune algorithm. Firstly, the user satisfaction evaluation function of electric vehicles is proposed based on the satisfaction optimization theory. Then, mathematical model of users’ satisfaction is established. Based on the model, a novel optimal algorithm is designed. Because of the improvement of cross and mutation, the proposed strategy is more accurate and convergent than the conventional immune algorithm. Furthermore, the proposed algorithm is implemented based on the immune algorithm, which offers the best site selection for EVs. Finally, Jinan City was used as an example to conduct a simulation experiment.

With the rapid development of electric vehicles, charging stations have become an important part of charging facilities. In this paper, based on the traditional P-median model and the maximum coverage model, a charging station site selection planning model is established. Then a method of location optimization is proposed, which combines the immune algorithm and the voronoi diagram. Finally, in order to obtain the optimal selection, we analyzed the voronoi diagram with the simulation results. This is a new method to optimize the location of charging stations by using advanced algorithms. The feasibility of the method is verified by an example.

The involvement of insulin/insulin-like growth factor signaling (IIS) pathways in the growth regulation of marine invertebrates remains largely unexplored. In this study, we used a fast-growing Pacific oyster (Crassostrea gigas) variety “Haida No.1” as the material with which to unravel the role of IIS systems in growth regulation in oysters. Systematic bioinformatics analyses allowed us to identify major components of the IIS signaling pathway and insulin-like peptide receptor (ILPR)-mediated signaling pathways, including PI3K-AKT, RAS-MAPK, and TOR, in C. gigas. The expression levels of the major genes in IIS and its downstream signaling pathways were significantly higher in “Haida No.1” than in wild oysters, suggesting their involvement in the growth regulation of C. gigas. The expression profiles of IIS and its downstream signaling pathway genes were significantly altered by nutrient abundance and culture temperature. These results suggest that the IIS signaling pathway coupled with the ILPR-mediated signaling pathways orchestrate the regulation of energy metabolism to control growth in Pacific oysters.

The involvement of insulin/insulin-like growth factor signaling (IIS) pathways in the growth regulation of marine invertebrates remains largely unexplored. In this study, we used a fast-growing Pacific oyster (Crassostrea gigas) variety “Haida No.1” as the material with which to unravel the role of IIS systems in growth regulation in oysters. Systematic bioinformatics analyses allowed us to identify major components of the IIS signaling pathway and insulin-like peptide receptor (ILPR)-mediated signaling pathways, including PI3K-AKT, RAS-MAPK, and TOR, in C. gigas. The expression levels of the major genes in IIS and its downstream signaling pathways were significantly higher in “Haida No.1” than in wild oysters, suggesting their involvement in the growth regulation of C. gigas. The expression profiles of IIS and its downstream signaling pathway genes were significantly altered by nutrient abundance and culture temperature. These results suggest that the IIS signaling pathway coupled with the ILPR-mediated signaling pathways orchestrate the regulation of energy metabolism to control growth in Pacific oysters.

With the gradual deep understanding of the tumorigenesis and development process, nanodrug are thought to have great prospects for individualized treatment of tumors. To deliver adequate concentration of active ingredients to targeted tissues, proteins are usually used as carriers to avoid clearance by the immune system. Herein, a new strategy is developed for preparation of the protein-functionalized targeting nanodrugs; different kinds of proteins (albumin, horseradish, transferrin, and ricin) can be quickly loaded in polyacrylic acid nanohydrogels (PAA-NGs) without discrimination within 1 min under the strong driving force of entropy; and the loading efficiency can reach 99% with about 50% loading content. Meanwhile, the activity of the released protein can be well retained. After oriented binding of the targeting agent on the surface of the nanocarriers by a unique and facile technique, the protein-loaded nanodrug exhibits excellent tumor cell uptake and targeting effect. The excellent targeting ability from the oriented binding is further proved by comparing with the non-oriented targeting system. With quick loading of the anti-tumor protein of ricin and oriented binding of transferrin protein (Tf), the targeting nanodrug (PAA-BB@Ricin/Tf) shows a remarkable anti-tumor effect. This study proves a new universal delivery and targeting strategy for improving the nanodelivery system, which has great potentials for clinical application.

Neuroendocrine hormones such as dopamine and insulin/insulin-like peptides play indispensable roles in growth regulation of animals, while the interplay between dopamine and insulin signaling pathways remains largely unknown in invertebrates. In the present study, we showed that tyrosine hydroxylase (TH), the rate-limiting enzyme of dopamine synthesis, was highly expressed in all tissues of the fast-growing oysters, and gradually increased with the development, which indicated the potential role of dopamine in growth regulation. Incubated with dopamine hydrochloride and insulin-like peptide recombinant proteins in vitro induced the expression of TH, suggesting a mutual regulatory relationship between insulin and dopamine signaling. Fasting and re-feeding experiments confirmed the role of TH in food intake regulation, also provide a clue about the potential regulatory relationship between the FoxO and TH. Further luciferase assay experiment confirmed that FoxO was involved in transcriptional regulation of TH gene through binding to its specific promoter region. This work provided insights into the crosstalk between dopamine and insulin signaling in growth control of mollusks.

Transmembrane proteins are involved in an array of stress responses, particularly in thermo-sensation and thermo-regulation. In this study, we performed a genome-wide identification and characterization of the Transient Receptor Potential (TRP) genes in the Pacific oyster (Crassostrea gigas) and investigated their expression profiles after heat stress to identify critical TRPs potentially associated with thermal regulation. A total of 66 TRP genes were identified in the C. gigas, which showed significant gene expansion and tandem duplication. Meta-analysis of the available RNA-Seq data generated from samples after acute heat stress revealed a set of heat-inducible TRPs. Further examination of their expression profiles under chronic heat stress, and comparison between C. gigas and C. angulata, two oyster species with different tolerance levels to heat stress, led to the identification of TRPC3.6, TRPC3.7, and TRPV4.7 as important TRPs involved in thermal regulation in oysters. This work provided valuable information for future studies on the molecular mechanism of TRP mediated thermal tolerance, and identification of diagnostic biomarker for thermal stress in the oysters.

The involvement of insulin/insulin-like growth factor (IIS) signaling pathway in growth regulation of marine invertebrates remains largely unexplored. In this study, we used a fast-growing Pacific oyster (Crassostrea gigas) variety “Haida No.1” as material to unravel the role of IIS system in growth regulation in oysters. Systematic bioinformatics analyses allowed to identify major components of IIS signaling pathway and insulin-like peptide receptor (ILPR) mediated signaling pathways, including PI3K-AKT, RAS-MAPK, and TOR, in C. gigas. Expression levels of the major genes in IIS and its downstream signaling pathways were significantly higher in “Haida No.1” than wild oysters, suggesting their involvement in growth regulation of C. gigas. Expression profiles of IIS and its downstream signaling pathway genes were significantly altered by nutrient abundance and culture temperature. These results suggested that IIS signaling pathway coupled with the ILPR mediated signaling pathways orchestrated energy homeostasis to regulate growth in the Pacific oyster.Research HighlightsILPR, IRS, IGFBPRP, and IGFALS genes were characterized in the C. gigas.Major genes of IIS signaling pathway were highly expressed in fast-growing C. gigas.IIS and downstream pathways participates in energy homeostasis of oysters.ILPR mediated signaling pathways orchestrate growth regulation in oysters.

Transmembrane proteins are involved in an array of stress responses, particularly in thermo-sensation and thermo-regulation. In this study, we performed a genome-wide identification and characterization of the Transient Receptor Potential (TRP) genes in the Pacific oyster (Crassostrea gigas) and investigated their expression profiles after heat stress to identify critical TRPs potentially associated with thermal regulation. A total of 66 TRP genes were identified in the C. gigas, which showed significant gene expansion and tandem duplication. Meta-analysis of the available RNA-Seq data generated from samples after acute heat stress revealed a set of heat-inducible TRPs. Further examination of their expression profiles under chronic heat stress, and comparison between C. gigas and C. angulata, two oyster species with different tolerance levels to heat stress, led to the identification of TRPC3.6, TRPC3.7, and TRPV4.7 as important TRPs involved in thermal regulation in oysters. This work provided valuable information for future studies on the molecular mechanism of TRP mediated thermal tolerance, and identification of diagnostic biomarker for thermal stress in the oysters.

Additional file 1: Figure S1. Structural formula of cypate. Figure S2. Hydrodynamic diameters of PMAA-Fe and Cyp-PMAA-Fe@MSCs and zeta potenials of PMAA-Fe, MSCs and Cyp-PMAA-Fe@MSCs. Figure S3. UV–vis-NIR spectra and typical photos of cypate with various concentrations. Figure S4. Absorbance of cypate with various concentrations at 785 nm. Figure S5. IR images of Cyp-PMAA-Fe@MSCs at 808 nm laser with various powers. Figure S6. Heating and cooling curve of Cyp-PMAA-Fe@MSCs. a The temperature change of Cyp-PMAA-Fe@MSCs response to 808 nm laser on and off in period of 1800s. b Linear regression of time versus –lnθ obtained from the cooling period of NIR laser off. Figure S7. H&E staining of tissue slices from several organs in various groups.

The insulin/insulin-like growth factor signaling (IIS) pathway is well-known in regulation of cell growth and proliferation in vertebrates, while its role in invertebrates such as mollusks remains largely unknown. In this study, we performed an extensive multi-omics data mining and identified four insulin-like peptide genes, including ILP, MIRP3, MIRP3-like and ILP7, in the Pacific oyster, Crassostrea gigas. Their potential roles in growth regulation were further investigated using the selectively bred fast-growing C. gigas variety "Haida No.1". Expression profiling and in situ hybridization of these insulin-like peptides suggested their distinct tissue-specific expression pattern, with dominant expression in the neural enrichment tissues such as labial palp, visceral ganglia, adductor muscle, and digestive gland. The expressions of insulin-like peptides were significantly altered by food abundance in a gene-specific fashion. The expression of ILP was reduced during fasting and increased after re-feeding, the expressions of MIRP3 and ILP7 were generally induced during fasting and down-regulated after re-feeding, while the expression of MIRP3-like was firstly up-regulated and then down-regulated during the fasting and re-feeding process. Furthermore, the expressions of all four insulin-like peptide genes were significantly suppressed at low temperature, in accordance with the growth inhibition. These results indicated that all four insulin-like peptides would play critical but different roles in regulation of growth in the oysters. This work provides valuable information for further investigation on growth regulation mechanism in mollusks and molecular assisted breeding of growth with other production traits in the Pacific oyster.