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The process pump as turbine (PPAT) serves as a crucial component for recovering high-pressure energy from mediums used in chemical and refining processes. Ensuring the long-term safe and stable operation of PPAT in high-temperature and high-pressure environments is essential, with pressure pulsation being one of its most significant external characteristic indicators. This study investigates the evolution of vortex structure distribution and the generation and propagation mechanisms of pressure pulsation in a two-stage PPAT operating in turbine mode. Results indicate that the uniformity of the pressure coefficient (Cp) gradient distribution is poorer in the first stage runner compared to the second stage, with a larger distribution area of high-strength vortices. In the draft tube, vortex strength increases with rising flow rates, and the flow around the circular cylinder on one side gradually develops to both sides. In the two-stage diffusers, the primary source of pressure pulsation is the dynamic and static interference effect between the two impellers and the corresponding diffuser tongue. The interstage interference with a frequency of n*15fn is most pronounced in the inflow runner, gradually weakening along the flow direction, and ultimately disappearing in the draft tube. In addition, more low-frequency signals with a frequency of 0.5fn are captured in the draft tube under large flow conditions, which is mainly generated by the vortex band in the draft tube. The low-frequency pulsation energy is high and the attenuation is slow, which has a great destructive effect on the energy recovery system of the PPAT.

Aiming at the problems of low soil cover and serious wear and tear of transmission parts in the soil lifting device of potato planters, this paper carries out innovative optimization design of its key parts based on TRIZ theory. The discrete element model of the soil model and the multi-body dynamic model of the soil lifting device are established, the upper scraping and lower scraping soil lifting devices are simulated respectively through the method of DEM-MBD coupling, and the working mechanism of the soil lifting device is further explored. The simulation results show that the lower scraping type lifting device has a large conveying capacity and a stable flow rate at the soil outlet. The results of the soil tank performance comparison test show that: the improved soil lifting device has a simple structure, large conveying capacity, stable flow rate, and fast flow speed and is not easily congested. It meets the standard requirements of dryland potato seedling strip mulching planting technology on mulching parameters and is of great significance for improving dryland food production on the Loess Plateau.

To address the escalating demand for power grid load regulation, pumped storage power stations must frequently switch between operational modes. As a key component of such stations, the pump turbine has seen extensive research on its steady-state flow behavior. However, the intricate dynamics of its transient flow have not yet been thoroughly examined. Notably, the no-load condition represents a quintessential transient state, the instability of which poses challenges for grid integration. Under certain extreme conditions, this could result in the impairment of the unit’s elements, interruption of its functioning, and endangerment of the security of the power station’s output as well as the stability of the power network’s operations. Thus, investigating the flow characteristics of pump turbines under no-load conditions is of significant practical importance. This paper focuses on the transient flow characteristics of a Weifang hydro-generator unit under no-load conditions, exploring the internal unsteady flow features and their underlying mechanisms. The study reveals that under no-load conditions, the runner channel is obstructed by a multitude of vortices, disrupting the normal pressure gradient within the runner and resulting in substantial hydraulic losses. Within the draft tube, a substantial reverse flow zone is present, predominantly along the walls. This irregular flow pattern within the tube generates a potent, stochastic pressure fluctuation. In addition to the interference frequencies of dynamic and static origins, the pressure pulsation frequency at each measurement point also encompasses a substantial portion of low-frequency, high-amplitude components.

With the development of global hydropower, the scale of hydropower stations is increasing, and the operating conditions are becoming more complex, so the stable operation of hydropower stations is very important. The vibration of the turbine unit will cause resonance in the powerhouse, and the structural stability of the powerhouse will be affected. Many scholars pay attention to the stability of the turbine unit operation, and there are few studies on the powerhouse of the hydropower station. Therefore, this paper relies on the Weifang Hydropower Station project to study key issues such as the tensile strength of concrete and how to arrange steel bars to increase the structural stability by changing the material properties through FEA. Three schemes are designed to evaluate the safety of the powerhouse structure when the turbine unit is running through the safety factor. Our findings indicate that the stress variation patterns observed on the inner surface of the powerhouse remain consistent across different operating scenarios. Notably, along the spiral line of the worm section, we observed that the stress levels on the vertical loop line decrease gradually with increasing distance from the inlet. Conversely, stress concentrations arise near the inlet and the tongue. Additionally, it has been noted that the likelihood of concrete cracking increases significantly at the tongue region.

To address the issues of the poor filling and clearing efficiency of spoon–chain potato seed dischargers, an optimization design was implemented in this study. Based on the motion characteristics of potatoes during the filling and transport processes, an inclination angle was set for the seed spoon cavity, and a seed-clearing brush was installed at the top of the seed discharger. A DEM-MBD coupled simulation model of the seed discharger was constructed. The working speed of the driving sprocket, the inclination angle of the seed spoon cavity, and the seed holding height were used as experimental factors, while the single-seed qualification rate, missed seed rate, and over-seeding rate were used as evaluation indices to conduct a quadratic regression orthogonal rotation combination experiment. This determined the optimal technical parameter combination for the best working performance. Based on the results of the DEM-MBD coupled simulation experiments, a response surface optimization test was conducted. The results showed that the optimal working performance was achieved when the working speed of the driving sprocket was 43 rpm, the inclination angle of the seed spoon cavity was 15°, and the seed holding height was 0.2 m. Under these conditions, the single-seed qualification rate was 95.28%, the missed seed rate was 0.92%, and the over-seeding rate was 3.80%. Further soil bin tests confirmed that, under the optimal working parameters, the relative deviations of all test indices from the response surface optimization test results were less than 2%. The research results provide new insights for the optimization design of spoon–chain potato seed-metering devices.

This paper introduces a modular, assembled steel beam-column flange connection joint that efficiently connects prefabricated beams and columns using high-strength bolts. It enables the rapid repair of damaged joints after earthquakes by replacing flange connectors and high-strength bolt groups. Four joint specimens with varying thicknesses and lengths of the inner flange sleeve, scaled at a 1:2 ratio, were fabricated to evaluate performance. These specimens were subjected to low circumferential reciprocal loads to investigate damage modes, hysteresis curves, skeleton curves, ductility performance, energy dissipation capacity, and seismic performance, including stiffness degradation. The test and analysis results reveal that the primary failure mode is characterized by bulging of the flange jacket cover, with damage concentrated in the plastic hinge zone at the beam end. The flange connection joint exhibits excellent load-bearing, rotational, and energy dissipation capacities. The ‘secondary strengthening’ feature significantly enhances joint load-bearing capacity, ductility performance, and energy dissipation, increasing overall safety redundancy. Increasing the thickness and length of the flange connector substantially improves seismic performance and enlarges the plastic development area.

In this study, the vibration characteristics of a bullet-loaded recorder’s vibration damping system under various preload conditions are investigated through theoretical analysis, numerical simulations, and experimental verification. The findings indicate that the inclusion of a polyurethane elastomer vibration damping buffer layer between the cartridge and the recorder, along with the application of a specific preload, significantly reduces the amplitude of vibration acceleration transmitted to the recorder’s interior. This, in turn, enhances the overload resistance of the cartridge’s internal circuit. Numerical simulation results and theoretical analysis suggest that increasing the preload on the buffer material between the elastomer and the recorder reduces both the frequency ratio and damping ratio of the damping system. This reduction further decreases the amplitude of vibration transmitted to the recorder. However, excessively high preload generates substantial compressive stress within the recorder under static conditions, intensifying during the projectile’s accelerated movement. As a consequence, deformation and damage occur to the internal circuitry. Therefore, ensuring that the recorder possesses the structural strength necessary to withstand increased preload is crucial. This balancing act improves the recorder’s resistance to shock, vibration, and overload, while also preventing excessive stress-induced damage.

We present an instructional approach to teaching causal inference using Bayesian networks and do-Calculus, which requires less prerequisite knowledge of statistics than existing approaches and can be consistently implemented in beginner to advanced levels courses. Moreover, this approach aims to address the central question in causal inference with an emphasis on probabilistic reasoning and causal assumption. It also reveals the relevance and distinction between causal and statistical inference. Using a freeware tool, we demonstrate our approach with five examples that instructors can use to introduce students at different levels to the conception of causality, motivate them to learn more concepts for causal inference, and demonstrate practical applications of causal inference. We also provide detailed suggestions on using the five examples in the classroom.

The Generation IV Lead-cooled fast reactor (LFR) take the liquid lead or lead-bismuth eutectic alloy (LBE) as the coolant of the primary cooling circuit. Combined with the natural characteristics of lead alloy and the design features of LFR, the system is the simplest and the number of equipment is the least, which reflects the inherent safety characteristics of LFR. The nuclear main coolant pump (MCP) is the only power component and the only rotating component in the primary circuit of the reactor, so the various operating characteristics of the MCP are directly related to the safety of the nuclear reactor. In this paper, various working conditions that may occur in the normal rotation (positive rotating) of the MCP and the corresponding internal flow characteristics are analyzed and studied, including the normal pump condition, the positive-flow braking condition and the negative-flow braking condition. Since the corrosiveness of LBE is proportional to the fluid velocity, the distribution of flow velocity in the pump channel will be the focus of this study. It is found that under the normal pump condition and positive-flow braking conditions, the high velocity region of the impeller domain appears at the inlet and outlet of the blade. At the same radius, the pressure surface is lower than the back surface, and with the increase of flow rate, the flow separation phenomenon is obvious, and the turbulent kinetic energy distribution in impeller and diffuser domain shows obvious near-wall property. Under the negative-flow braking condition, there is obvious flow separation in the impeller channel.

Background and purpose: Free breathing (FB) positron emission tomography (PET) images are routinely used in radiotherapy for lung cancer patients. Respiration-induced artifacts in these images compromise treatment response assessment and obstruct clinical implementation of dose painting and PET-guided radiotherapy. The purpose of this study is to develop a blurry image decomposition (BID) method to correct motion-induced image-reconstruction errors in FB-PETs. Materials and methods: Assuming a blurry PET is represented as an average of multi-phase PETs. A four-dimensional computed-tomography image is deformably registered from the end-inhalation (EI) phase to other phases. With the registration-derived deformation maps, PETs at other phases can be deformed from a PET at the EI phase. To reconstruct the EI-PET, the difference between the blurry PET and the average of the deformed EI-PETs is minimized using a maximum-likelihood expectation–maximization algorithm. The developed method was evaluated with computational and physical phantoms as well as PET/CT images acquired from three patients. Results: The BID method increased the signal-to-noise ratio from 1.88 ± 1.05 to 10.5 ± 3.3 and universal-quality index from 0.72 ± 0.11 to 1.0 for the computational phantoms, and reduced the motion-induced error from 69.9% to 10.9% in the maximum of activity concentration and from 317.5% to 8.7% in the full width at half maximum of the physical PET-phantom. The BID-based corrections increased the maximum standardized-uptake values by 17.7 ± 15.4% and reduced tumor volumes by 12.5 ± 10.4% on average for the three patients. Conclusions: The proposed image-decomposition method reduces respiration-induced errors in PET images and holds potential to improve the quality of radiotherapy for thoracic and abdominal cancer patients.

A 3D reconstruction method using two spherical embeddings to resolve projection angles of the cryo-EM images is shown to improve the initial model reconstruction for single-particle analysis.

The role of sperm membrane proteins DCST1 and DCST2 in gamete fusion is extended to zebrafish in showing that these interdependent proteins are required for sperm-egg binding.

Abstract Lenvatinib is the first target drug approved for advanced hepatocellular carcinoma (HCC). However, the development of drug resistance is common, and the mechanisms of lenvatinib resistance and resistant targets in HCC are poorly understood. By using CRISPR/Cas9 library screening, we screened out two key resistance genes, neurofibromin 1(NF1), and dual specificity phosphatase 9 (DUSP9), as critical drivers for lenvatinib resistance in HCC. With RNAi knockdown and CRISPR/Cas9 knockout models, we further clarified the mechanisms by which NF1 loss reactivates the PI3K/AKT and MAPK/ERK signaling pathways, while DUSP9 loss activates the MAPK/ERK signaling pathways, thereby inactivating FOXO3, followed by degradation of FOXO3, finally induced lenvatinib resistance. We also screened out trametinib, a small molecule pathway inhibitor for MEK, that can be used to reverse resistance induced by NF1 and DUSP9 loss in HCC cells. Trametinib was still able to halt HCC growth even when NF1 was knocked out in mice. Collectively, the findings indicate that NF1 and DUSP9 takes critical role in lenvatinib resistance and may be novel specific targets and predictive markers for lenvatinib resistance in HCC.

Three-dimensional (3D) reconstruction in single-particle cryo-electron microscopy (cryo-EM) is a significant technique for recovering the 3D structure of proteins or other biological macromolecules from their two-dimensional (2D) noisy projection images taken from unknown random directions. Class averaging in single-particle cryo-EM is an important procedure for producing high-quality initial 3D structures, where image alignment is a fundamental step. In this paper, an efficient image alignment algorithm using 2D interpolation in the frequency domain of images is proposed to improve the estimation accuracy of alignment parameters of rotation angles and translational shifts between the two projection images, which can obtain subpixel and subangle accuracy. The proposed algorithm firstly uses the Fourier transform of two projection images to calculate a discrete cross-correlation matrix and then performs the 2D interpolation around the maximum value in the cross-correlation matrix. The alignment parameters are directly determined according to the position of the maximum value in the cross-correlation matrix after interpolation. Furthermore, the proposed image alignment algorithm and a spectral clustering algorithm are used to compute class averages for single-particle 3D reconstruction. The proposed image alignment algorithm is firstly tested on a Lena image and two cryo-EM datasets. Results show that the proposed image alignment algorithm can estimate the alignment parameters accurately and efficiently. The proposed method is also used to reconstruct preliminary 3D structures from a simulated cryo-EM dataset and a real cryo-EM dataset and to compare them with RELION. Experimental results show that the proposed method can obtain more high-quality class averages than RELION and can obtain higher reconstruction resolution than RELION even without iteration.

The reactor coolant pump (RCP) is the only rotating equipment in the primary circuit system of a nuclear power plant and the “heart” of the nuclear reactor. The L formula is defined, and the L/himp is introduced to study the influence of impeller blade type on the performance of the RCP. Twenty groups of models are designed, the concept of arc height ratio is proposed from the perspective of himp and L, and the distribution of internal entropy production within the impeller of the RCP under different Ls and himps of the impeller blade type is analyzed. The results show that when himp remains un-changed and L increases, the low-pressure area at the inlet of the impeller expands while the high-pressure area at the outlet decreases under the design flow or large flow conditions. The smoother blade profile reduces the occurrence of secondary flow phenomena and makes the RCP pressure distribution more uniform. Under design flow and large flow conditions, smaller L/himp and higher himp lead to higher efficiency and head performance. However, higher efficiency and lower head performance can be achieved under small flow conditions with larger L/himp and lower himp.

Lead alloy is used as coolant in Lead-based cooled Fast Reactor (LFR). The natural characteristics of lead alloy are combined with the simple structural design of LFR. This constitutes the inherent safety characteristics of LFR. The main work of this paper is to take the main coolant pump (MCP) in the lead-cooled fast reactor (LFR) as the research object, and to study the flow pattern distribution of the internal flow field under the reverse rotation pump condition, the reverse rotation positive-flow braking condition and the reverse rotation negative-flow braking condition. In this paper, the double-outlet volute type and the space guide vane are selected as the potential designs of the CLEAR-I MCP. In this paper, the CFD method is used to study the potential reverse accident of the MCP. It is found that the highest flow velocity in the impeller appears at the impeller outlet, and the Q-H curves of the two design programs basically coincide. The space guide vane type MCP has better hydraulic performance under the reverse rotation positive-flow condition, the Q-H curves of the two designs gradually separate with increasing flow rate, and the maximum flow velocity inside the space guide vane type MCP is obviously lower than that of the double-outlet volute type. For the reverse rotation test of MCP, only the condition of the forward rotating pump of the main coolant pump is tested and verified. For the simulation of the MCP in LBE medium, it proved that the turbulence model and basic settings selected in the simulation are reliable.

Heterogeneous three-dimensional (3D) reconstruction in single-particle cryo-electron microscopy (cryo-EM) is an important but very challenging technique for recovering the conformational heterogeneity of flexible biological macromolecules such as proteins in different functional states. Heterogeneous projection image classification is a feasible solution to solve the structural heterogeneity problem in single-particle cryo-EM. The majority of heterogeneous projection image classification methods are developed using supervised learning technology or require a large amount of a priori knowledge, such as the orientations or common lines of the projection images, which leads to certain limitations in their practical applications. In this paper, an unsupervised heterogeneous cryo-EM projection image classification algorithm based on autoencoders is proposed, which only needs to know the number of heterogeneous 3D structures in the dataset and does not require any labeling information of the projection images or other a priori knowledge. A simple autoencoder with multi-layer perceptrons trained in iterative mode and a complex autoencoder with residual networks trained in one-pass learning mode are implemented to convert heterogeneous projection images into latent variables. The extracted high-dimensional features are reduced to two dimensions using the uniform manifold approximation and projection dimensionality reduction algorithm, and then clustered using the spectral clustering algorithm. The proposed algorithm is applied to two heterogeneous cryo-EM datasets for heterogeneous 3D reconstruction. Experimental results show that the proposed algorithm can effectively extract category features of heterogeneous projection images and achieve high classification and reconstruction accuracy, indicating that the proposed algorithm is effective for heterogeneous 3D reconstruction in single-particle cryo-EM.

Background: Recent studies highlight the carcinogenesis role of SHC-adaptor protein 1 (SHC1) in cancer initiation, development, and progression. However, its aberrant expression, diagnostic and prognostic value remain unknown in a variety of tumors.Methods: The SHC1 expression profiles were analyzed using GTEx database, TCGA database, Oncomine and CPTAC database. The survival analysis was conducted using GEPIA2, Kaplan-Meier Plotter, UALCAN, and PrognoScan. The diagnostic values of SHC1 were calculated with the “pROC” package in R software. The genetic alteration of SHC1 and mutations were analyzed using cBioPortal. TIMER2 was employed to estimate the correlations between SHC1 expression and tumor-infiltrating immune cells in the TCGA cohort. Enrichment analysis of SHC1 was conducted using the R package “clusterProfiler.”Results: SHC1 was ubiquitously highly expressed and closely associated with worse prognosis of multiple major cancer types (all p < 0.05). Further, SHC1 gene mutations were strongly linked to poor OS and DFS in SKCM (all p < 0.05). An enhanced phosphorylation level of SHC1 at the S139 site was observed in clear cell RCC. Additionally, the results revealed SHC1 expression was strongly linked to TMB, MMRs, MSI, TAMs, DNA methylation, m6A RNA methylation, tumor-associated immune infiltration, and immune checkpoints in multiple cancers (all p < 0.05). In addition, the results of the ROC analysis indicated the SHC1 exhibited strong diagnostic capability for KICH (AUC = 0.92), LIHC (AUC = 0.95), and PAAD (AUC = 0.95). Finally, enrichment analysis indicated that SHC1 may potentially involve in the regulation of numerous signaling pathways in cancer metabolism and protein phosphorylation-related functions.Conclusions: These findings highlight that SHC1 plays an important role in the tumor immune microenvironment, and SHC1 has been identified to have prognostic and diagnostic value in multiple cancers. Thus, SHC1 is a potential target for cancer immunotherapy and effective prognostic and diagnostic biomarker.

Tracking a fast-moving sound source in the air in an acoustic way has rarely been seen in the current literature. The speed of the source in the air is often not negligible compared to the speed of sound in the air. Time-varying propagation distance and noticeable Doppler effect also bring difficulties to traditional tracking methods with the time difference of arrival (TDOA) and direction of arrival (DOA). In this paper, we propose a particle filtering framework with relative Doppler stretch. We utilize the propagation delayed measurement (PDM) model and correct the posterior probability in traditional particle filter with propagation delayed state being a bridge. This method avoids the complex pre-processing of the raw acoustic signals. The simulation results show that the algorithm has an expected performance and is superior to the existing methods. The whole tracking process which starts from raw signals is shown for the first time, and the key factors affecting the pre-processing of the raw signals that are not mentioned in other articles are also discussed in this paper.

Fertilization occurs as the culmination of multi-step complex processes. First, mammalian spermatozoa undergo the acrosome reaction to become fusion-competent. Then, the acrosome-reacted spermatozoa penetrate the zona pellucida and adhere to and finally fuse with the egg plasma membrane. IZUMO1 is the first sperm protein proven to be essential for sperm-egg fusion in mammals, as Izumo1 knockout mouse spermatozoa adhere to but fail to fuse with the oolemma. However, the IZUMO1 function in other species remains largely unknown. Here, we generated Izumo1 knockout rats by CRISPR/Cas9 and found the male rats were infertile. Unlike in mice, Izumo1 knockout rat spermatozoa failed to bind to the oolemma. Further investigation revealed that the acrosome-intact sperm binding conceals a decreased number of the acrosome-reacted sperm bound to the oolemma in Izumo1 knockout mice. Of note, we could not see any apparent defects in the binding of the acrosome-reacted sperm to the oolemma in the mice lacking recently found fusion-indispensable genes, Fimp, Sof1, Spaca6, or Tmem95. Collectively, our data suggest that IZUMO1 is required for the sperm-oolemma binding prior to fusion at least in rat.

The AP1000 reactor coolant pump is a vertical shielded-mixed flow pump, is the most important coolant power supply and energy exchange equipment in nuclear reactor primary circuit system, whose steady-state and transient performance affect the safety of the whole nuclear island. Moreover, safety demonstration of reactor coolant pump is the most important step to judge whether it can be practiced, among which software simulation is the first step of theoretical verification. This paper mainly introduces the fluid-solid coupling simulation method applied to reactor coolant pump, studying the feasibility of simulation results based on workbench fluid-solid coupling technology. The study found that: for the unsteady calculations of the pure liquid media, the average head of the reactor coolant pump with bidirectional fluid-solid coupling decreases to a certain extent. And the coupling result is closer to the real experimental value. The large stress and deformation of rotor under different flow conditions are mainly distributed on impeller and idler, and the stress concentration mainly occurs at the junction of front cover plate and blade outlet. Among the factors that affect the dynamic stress change of rotor, the pressure load takes a dominant position. Keywords: Reactor coolant pump, Bidirectional fluid-solid coupling, Safety demonstration, Steady state condition, Nomenclature

Since the first nuclear reactor first critical, nuclear systems has gone through four generations of history, and the fourth generation nuclear system will be truly realized in the near future. The notions of SVBR and lead-bismuth eutectic alloy coolant put forward by Russia were well received by the international nuclear science community. Lead-bismuth eutectic alloy with the ability of the better neutron economy, the low melting point, the high boiling point, the chemical inertness to water and air and other features, which was considered the most promising coolant for the 4th generation nuclear reactors. This study mainly focuses on the structural design optimization of the 4th-generation reactor coolant pump, including analysis of external characteristics, inner flow, and transient characteristic. It was found that: the reactor coolant pump with a central symmetrical dual-outlet volute structure has better radial-direction balance, the pump without guide vane has better hydraulic performance, and the pump with guide vanes has worse torsional vibration and pressure pulsation. This study serves as experience accumulation and technical support for the development of the 4th generation nuclear energy system. Keywords: LBE, Reactor coolant pump, SVBR, Hydraulic characteristics, Structure design

Introduction Exposure to the fine particulate matter PM2.5 is strongly associated with atherosclerotic diseases, creating considerable public concern. Nevertheless, the mechanisms have not been fully elucidated. We exposed atherosclerosis-prone apoE-deficient mice to PM2.5 to begin investigating these mechanisms. Material and methods Thirty-two 8-week-old male apoE –/– mice were divided to two groups fed with high-fat diet: a control group instilled with 0.9% saline, and an experimental group instilled with PM2.5 (30 mg/kg/day) for 8 weeks. We measured PM2.5 in whole blood by the ICP-MS method, and lipids and inflammatory factors by standard methods. The whole descending arteries were stained with oil red O; Aortic roots were stained with Movat, Sirius Red and immunohistochemical stains for pathological analysis; Brachiocephalic arteries for scanning electron microscopy, the descending arteries for Q-PCR. Echocardiography was used to evaluate cardiac function. Results In PM2.5 group, we observed elevated heavy metal components, consistent with higher amounts of platelets in total blood. The PM2.5 group also had elevated serum inflammatory factor levels. Finally, the PM2.5 group showed larger atherosclerotic plaques (p = 0.0231), higher numbers of lesion macrophages (p = 0.0183), greater injury to endothelial layers with greater adherence of platelets and leukocytes, elevated inflammatory factor levels, the NAD(P)H oxidase subunits p22phox and p47phox (p = 0.0079 and p = 0.0294), the M1/M2 associated markers IL-6, TNF-α (p = 0.0291, p = 0.0286), iNOS, IL-12 (p = 0.0122 and p = 0.0280) and arginase-1, and CD206 (p = 0.0216 and p = 0.0317). Conclusions PM2.5 exposure activated circulating leukocytes, platelets and associated inflammatory factors, contributing to the progression of atherosclerosis in apoE –/– mice.

Ultraconserved RNAs are a class of long non-coding RNAs whose functions are yet to be identified. Here Guo and colleagues show that an ultraconserved RNA uc.372 promotes lipogenesis and lipid accumulation within the hepatocytes by suppressing the maturation of miR-195/miR-4668 that inhibits lipogenic gene expression.

Galeolaria gemineoa is a sessile broadcast-spawning marine invertebrate, whose spermatozoa have been regarded as a sensitive indicator for water quality monitoring. In this study, 10 steps of spermiogenesis have been identified at the ultrastructural level and this differentiation process has been recapitulated in vitro up to the point of spermiogenesis (step 7-9 spermatids). On completion of the second meiosis, newly formed spermatids were detached from the seminiferous epithelium and released to the lumen of each germinal chamber. These spermatids were present in pairs and interconnected by a cytoplasmic bridge throughout the entire spermiogenic process. On the basis of morphological events such as formation of the acrosome, elongation of the flagellum, and condensation of the nucleus, spermiogenesis has been temporally divided into Golgi phase, acrosomal phase and maturation phase. During the Golgi phase, proacrosomal vesicles appeared at the posterior pole of the spermatids and gradually fused into a proacrosomal vacuole. Simultaneously, the distal centriole docked onto the plasma membrane and gave rise to a formative flagellum. The acrosomal phase was characterised by differentiation of the acrosome, condensation of the chromatin and formation of a mitochondrial sheath surrounding the initial portion of the flagellum. During the maturation phase, the fully differentiated acrosome migrated to the anterior pole and excess cytoplasm was extruded from the spermatids in the form of residual bodies. In addition, we successfully induced step 1-3 spermatids to differentiate into the step 7-9 spermatids in both male germinal fluid and 10% foetal bovine serum in RPMI 1640 medium, but failed to replicate this process in female or boiled male germinal fluids. This finding supports our concept that spermatid differentiation in this species is dependent on intrinsic developmental programming and does not require input from accompanying nurse cells.

For the first time, this study has systemically investigated the male reproductive system in a sessile broadcast-spawning marine invertebrate, Galeolaria caespitosa (Polychaeta: Serpulidae), which has significant potential as a bio-indicator species of coastal marine pollution. The abdomen of G. caespitosa was divided by intersegmental septa into over 80 trunk segments. Each segment served as a germinal chamber with a C-shaped gonadal arrangement consisting of several distinct compartments: a seminiferous epithelium (SE) compartment located in the centre of the chamber, with each of its two ends connecting to a nurse cell (NC) compartment and then an efferent duct (ED) compartment. The SE compartment contained a multilayered seminiferous epithelium where spermatogenesis was initiated. Spermatids were released in pairs into the lumen of the SE compartment and then transported to the NC compartment where they underwent spermiogenesis with the support of secretory vesicles released by the nurse cells. Spermatozoa were stored in the ED compartment and subsequently released into the seawater through the vas deferens. Unlike vertebrates where germ cells differentiated in close proximity to the nurse cell population (i.e. Sertoli cells), the spermatogenic cells of G. caespitosa exhibited no direct contact with supporting cells at any spermatogenic stage. This finding suggested that the spermatogenesis in G. caespitosa was more dependent on intrinsic developmental programming than most species. Notwithstanding such differences, there were clear parallels between the male reproductive system of G. caespitosa and mammals, in terms of the structure and function. The independence of spermatogenic cells from supporting cells in G. caespitosa raised the possibility of inducing spermiogenesis in vitro, which would provide a useful tool to dissect the mechanisms underlying this complex cell differentiation process in invertebrates and other higher order animals.

A silver nanoparticle-modified evanescent field optical fiber sensor based on a MEMS microchannel chip has been successfully fabricated. Experimental results show that the sensor response decreases linearly with increasing concentration of analyte. Over a range of methylene blue concentrations from 0 to 0.4 μmol/mL, the sensor response is linear (R = 0.9496). A concentration variation of 0.1 μmol/mL can cause an absorbance change of 0.402 dB. Moreover, the optical responses of the same sensing fiber without decoration and modified with silver nanoparticles have also been compared. It can be observed that the output intensity of the Ag nanoparticle-modified sensor is enhanced and the sensitivity is higher. Meanwhile, the absorbance spectra are found to be more sensitive to concentration changes compared to the spectra of the peak wavelength.

Dysfunction of β-cell is one of major characteristics in the pathogenesis of type 2 diabetes. The combination of obesity and type 2 diabetes, characterized as 'diabesity', is associated with elevated plasma free fatty acids (FFAs). Oxidative stress has been implicated in the pathogenesis of FFA-induced β-cell dysfunction. However, molecular mechanisms linking between reactive oxygen species (ROS) and FFA-induced β-cell dysfunction and apoptosis are less clear. In the present study, we test the hypothesis that NOX2-derived ROS may play a critical role in dysfunction and apoptosis of β-cells induced by FFA. Our results show that palmitate and oleate (0.5 mmol/L, 48 h) induced JNK activation and AKT inhibition which resulted in decreased phosphorylation of FOXO1 following nuclear localization and the nucleocytoplasmic translocation of PDX-1, leading to the reducing of insulin and ultimately dysfunction of pancreatic NIT-1 cells. We also found that palmitate and oleate stimulated apoptosis of NIT-1 cells through p38MAPK, p53 and NF-κB pathway. More interestingly, our data suggest that suppression of NOX2 may restore FFA-induced dysfunction and apoptosis of NIT-1 cells. Our findings provide a new insight of the NOX2 as a potential new therapeutic target for preservation of β-cell mass and function.

In this study, a series of hypervelocity impact tests were carried out based on a two-stage light gas gun, and the sequence spectrum and radiation evolution data of the impact products under different impact conditions were obtained. The diameter of the projectile is 3e5 mm, the impact velocity is 3.13e6.58 km/s, and the chamber pressure is 0.56e990 Pa. The spectrum of ejected debris cloud in the 250e310 nm band were obtained using a transient spectral measurement system and a multi-channel radiometer measurement system. The test results reveal that the flash radiation intensity increases as a power function with the kinetic energy of the impact. Furthermore, the peak value of the line spectrum decreases as the chamber vacuum degree increases, while the radiation width gradually expands. The line spectrum in the spectral characterization curve corresponds to the ejected debris clouds splitting phase, which does not produce significant line spectrum during material fragmentation and is dominated by the continuum spectrum produced by blackbody radiation. There will appear one or three characteristic peaks in the flash radiation time curve, the first and second peaks correspond to the penetration phase and the third peak corresponds to the expansion phase of the ejected debris clouds on the time scale, the first and second peaks are more sensitive to the chamber vacuum degree, and when the pressure is higher than 99 Pa, the first and second characteristic peaks will disappear. The radiant heat attenuation of the flash under different impact conditions is significantly different, the attenuation exponent has a power function relationship with the impact velocity and the chamber vacuum degree, while the attenuation exponent has a linear relationship with the diameter of the projectile, the specific expression of the attenuation exponent is obtained by fitting. The findings from this research can serve as a valuable reference for remote diagnostic technologies based on flash radiation characteristics. [ABSTRACT FROM AUTHOR]