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Aims: This study aimed, through bioinformatics analysis and in vitro experiment validation, to identify the key extracellular proteins of intervertebral disc degeneration (IDD). Methods: The gene expression profile of GSE23130 was downloaded from the Gene Expression Omnibus (GEO) database. Extracellular protein-differentially expressed genes (EP-DEGs) were screened by protein annotation databases, and we used Gene Ontology (GO) and the Kyoto Encyclopedia of Genes and Genomes (KEGG) to analyze the functions and pathways of EP-DEGs. STRING and Cytoscape were used to construct protein-protein interaction (PPI) networks and identify hub EP-DEGs. NetworkAnalyst was used to analyze transcription factors (TFs) and microRNAs (miRNAs) that regulate hub EP-DEGs. A search of the Drug Signatures Database (DSigDB) for hub EP-DEGs revealed multiple drug molecules and drug-target interactions. Results: A total of 56 EP-DEGs were identified in the differential expression analysis. EP-DEGs were enriched in the extracellular structure organization, ageing, collagen-activated signalling pathway, PI3K-Akt signalling pathway, and AGE-RAGE signalling pathway. PPI network analysis showed that the top ten hub EP-DEGs are closely related to IDD. Correlation analysis also demonstrated a significant correlation between the ten hub EP-DEGs (p＜0.05), which were selected to construct TF–gene interaction and TF–miRNA coregulatory networks. In addition, ten candidate drugs were screened for the treatment of IDD. Conclusion: The findings clarify the roles of extracellular proteins in IDD and highlight their potential as promising novel therapeutic targets. Cite this article: Bone Joint Res 2023;12(9):522–535.

Abstract Background While previous genome-wide association studies (GWAS) have identified multiple risk variants for migraine, there is a lack of evidence about how these variants contribute to the development of migraine. We employed an integrative pipeline to efficiently transform genetic associations to identify causal genes for migraine. Methods We conducted a proteome-wide association study (PWAS) by combining data from the migraine GWAS data with proteomic data from the human brain and plasma to identify proteins that may play a role in the risk of developing migraine. We also combined data from GWAS of migraine with a novel joint-tissue imputation (JTI) prediction model of 17 migraine-related human tissues to conduct transcriptome-wide association studies (TWAS) together with the fine mapping method FOCUS to identify disease-associated genes. Results We identified 13 genes in the human brain and plasma proteome that modulate migraine risk by regulating protein abundance. In addition, 62 associated genes not reported in previous migraine TWAS studies were identified by our analysis of migraine using TWAS and fine mapping. Five genes including ICA1L, TREX1, STAT6, UFL1, and B3GNT8 showed significant associations with migraine at both the proteome and transcriptome, these genes are mainly expressed in ependymal cells, neurons, and glial cells, and are potential target genes for prevention of neuronal signaling and inflammatory responses in the pathogenesis of migraine. Conclusions Our proteomic and transcriptome findings have identified disease-associated genes that may give new insights into the pathogenesis and potential therapeutic targets for migraine.

This paper proposed a high-sensitivity phase imaging eddy current magneto-optical (PI-ECMO) system for carbon fiber reinforced polymer (CFRP) defect detection. In contrast to other eddy current-based detection systems, the proposed system employs a fixed position excitation coil while enabling the detection point to move within the detection region. This configuration effectively mitigates the interference caused by the lift-off effect, which is commonly observed in systems with moving excitation coils. Correspondingly, the relationship between the defect characteristics (orientation and position) and the surface vertical magnetic field distribution (amplitude and phase) is studied in detail by theoretical analysis and numerical simulations. Experiments conducted on woven CFRP plates demonstrate that the designed PI-ECMO system is capable of effectively detecting both surface and internal cracks, as well as impact defects. The excitation current is significantly reduced compared with traditional eddy current magneto-optical (ECMO) systems.

Spinal cord injury can lead to severe motor, sensory and autonomic nervous dysfunctions. However, there is currently no effective treatment for spinal cord injury. Neural stem cells and progenitor cells, bone marrow mesenchymal stem cells, olfactory ensheathing cells, umbilical cord blood stem cells, adipose stem cells, hematopoietic stem cells, oligodendrocyte precursor cells, macrophages and Schwann cells have been studied as potential treatments for spinal cord injury. These treatments were mainly performed in animals. However, subtle changes in sensory function, nerve root movement and pain cannot be fully investigated with animal studies. Although these cell types have shown excellent safety and effectiveness in various animal models, sufficient evidence of efficacy for clinical translation is still lacking. Cell transplantation should be combined with tissue engineering scaffolds, local drug delivery systems, postoperative adjuvant therapy and physical rehabilitation training as part of a comprehensive treatment plan to provide the possibility for patients with SCI to return to normal life. This review summarizes and analyzes the clinical trials of cell transplantation therapy in spinal cord injury, with the aim of providing a rational foundation for the development of clinical treatments for spinal cord injury.

ObjectiveThe brain compensation mechanism in postoperative delirium (POD) has not been reported. We uncovered the mechanism by exploring the association between POD and glioma grades, and the relationship between preoperative brain structural and functional compensation with POD in patients with frontal glioma.MethodsA total of 335 adult patients with glioma were included. The multivariable analysis examined the association between tumor grade and POD. Then, 20 patients with left frontal lobe glioma who had presurgical structural and functional MRI data and Montreal Cognitive Assessment (MoCA) in this cohort were analyzed. We measured the gray matter volume (GMV) and functional connectivity (FC) in patients with (n = 8) and without (n = 12) POD and healthy controls (HCs, n = 29) to detect the correlation between the structural and functional alteration and POD.ResultsThe incidence of POD was 37.3%. Multivariable regression revealed that high-grade glioma had approximately six times the odds of POD. Neuroimaging data showed that compared with HC, the patients with left frontal lobe glioma showed significantly increased GMV of the right dorsal lateral prefrontal cortex (DLPFC) in the non-POD group and decreased GMV of right DLPFC in the POD group, and the POD group exhibited significantly decreased FC of right DLPFC, and the non-POD group showed the increasing tendency. Partial correlation analysis showed that GMV in contralesional DLPFC were positively correlated with preoperative neurocognition, and the GMV and FC in contralesional DLPFC were negatively correlated with POD.ConclusionsOur findings suggested that insufficient compensation for injured brain regions involving cognition might be more vulnerable to suffering from POD.

Abstract Background Systematic evaluation of the successful heartbeat recovery rate (HRR) in patients during the platinum ten minutes after cardiac arrest. Methods The databases of CNKI (January 1979–March 2019), Chongqing VIP (January 1989–March 2019), Wanfang (January 1990–March 2019) and Web of Science (1900-May 2020) were searched. To collect the clinical data of patients with cardiac arrest before hospitalization and analyze the cardiopulmonary resuscitation (CPR) at different times. Literature selection and data extraction were carried out by two researchers independently, and the meta package of R software (version 3. 61) was used for analysis. Results A total of 116 papers met the inclusion criteria, including 37,181 patients. Of these patients, 3367 had their heartbeats successfully restored. The results showed a high degree of heterogeneity (χ 2 = 6999.21, P 15 min group. The HRR were 0.417 (0.341–0.496), 0.143 (0.104–0.193), 0.049 (0.034–0.069), and 0.022 (0.009–0.051), respectively. The HRR was higher in the 0 to ≤5 min group than in the 5 to ≤10 min group, the 10 to ≤15 min group and the > 15 min group. There was no difference between the 10 to ≤15 min group and the > 15 min group. (3) When the groups were stratified with the cutoff of 10 min, the ≤10 min group HRR [0.250 (0.202–0.306)] was higher than the > 10 min group rate [0.041 (0.029–0.057)]. (4) The HRR of the telephone guidance group was [0.273 (0.227–0.325)] lower than that of the 0 to ≤5 min group [0.429 (0.347–0.516)] but higher than that of the 5 to ≤10 min group, the 10 to ≤15 min group, and the > 15 min group. (5) The HRR of the witness group [0.325 (0.216–0.458)] was not different from that of the 0 to ≤5 min group, but it was higher than those of the 5 to ≤10 min group, the 10 to ≤15 min group and the > 15 min group. (6) There was no significant difference HRR between the witnessed group, the telephone guidance group and the ≤10 min group. Conclusions (1) The HRR is time-sensitive, and early rescue can improve it. (2) CPR performed within the platinum ten minutes must be executed by the public, and other forces are auxiliary. (3) The concept of peri-cardiac arrest period (PCAP) should be established and improved to guide CPR.

Naoxintong Capsule (NXT), a Chinese medicine, has been widely used for the treatment of cardiovascular diseases in clinic. The present study was designed to clarify the effects of NXT on haemorheology, blood coagulation, hepatic function, antioxidant capacity and inflammatory responses in a rat model of blood stasis syndrome. Rats were placed in ice-cold water for 5 min during the time interval of two adrenaline injections to induce blood circulation disorders after intragastric administration of 10 consecutive days. Blood was collected from abdominal aorta to determine whole blood viscosity (WBV), plasma viscosity (PV), activated partial thromboplastin time (APTT), prothrombin time (PT), fibrinogen (FIB), total antioxidant capacity (T-AOC), superoxide dismutase (SOD), malonaldehyde (MDA), alanine transaminase (ALT), aspartate transaminase (AST), interleukin-1β (IL-1β), interleukin-6 (IL-6), platelet activating factor (PAF). The results showed that NXT could significantly decrease the WBV, PV, FIB and prolong the APTT and PT. Meanwhile, NXT significantly decreased the serum contents of MDA, IL-1β, IL-6, ALT, AST and PAF, while elevating T-AOC and SOD levels. These results indicate that NXT could improve coagulation function, enhance antioxidant capacity and suppress inflammation, which might partly account for its mechanisms of action in preventing and treating cardiovascular diseases.

Context Naoxintong Capsule (NXT), a Chinese medicine, has been widely used for the treatment of coronary heart disease (CHD) in clinics. Objective This study evaluated the cardioprotective effects of NXT alone and in combination with ticagrelor (TIC) and atorvastatin (ATO). Materials and methods Qi deficiency and blood stasis rats were established by 8 weeks high fat diet feeding and 16 days exhaustive swimming and randomly divided into seven groups, that is, NXT (250, 500 and 1000 mg/kg/d), TIC (20 mg/kg/d), ATO (8 mg/kg/d), NXT (500 mg/kg/d)+TIC (20 mg/kg/d) and NXT (500 mg/kg/d)+ATO (8 mg/kg/d) group, with oral administration for 12 weeks. The contents of TC, TG, LDL-C, HDL-C, IL-1β, IL-6, IL-8, TNF-α, AST, ALT, SOD, MDA, CK-MB, LDH, TXA2, PGI2, IgA, IgG, IgM and C3 in serum were measured. Results NXT + TIC group was significantly superior to the TIC group in decreasing the levels of TC (4.34 vs. 5.54), TG (3.37 vs. 4.66), LDL-C (1.21 vs. 1.35), LDH (4919.71vs. 5367.19) and elevating SOD level (248.54 vs. 192.04). NXT + ATO group was significantly superior to the ATO group in decreasing the levels of AST (195.931 vs. 241.63), ALT (71.26 vs. 83.16), LDH (4690.05 vs. 5285.82), TXA2 (133.73 vs. 158.67), IgG (8.08 vs. 9.80), C3 (2.03 vs. 2.35) and elevating the levels of HDL-C (1.19 vs. 0.91), SOD (241.91vs. 209.49). Conclusions The present findings demonstrate that the combined use of NXT with TIC and ATO had better integrated regulating effects than TIC and ATO, respectively. The mechanism of action requires further research.

Moyamoya-like vasculopathy, the “puff of smoke”-like small vessels in the brain, is initially identified in patients with Moyamoya disease (MMD), a rare cerebrovascular disease, and later found in patients with various types of neurological conditions, including Down syndrome, Stroke, and vascular dementia. It is thus of interest to understand how this vasculopathy is developed. Here, we provided evidence for cortical astrocytic neogenin (NEO1) deficiency to be a risk factor for its development. NEO1, a member of deleted in colorectal cancer (DCC) family netrin receptors, was reduced in brain samples of patients with MMD. Astrocytic Neo1-loss resulted in an increase of small blood vessels (BVs) selectively in the cortex. These BVs were dysfunctional, with leaky blood-brain barrier (BBB), thin arteries, and accelerated hyperplasia in veins and capillaries, resembled to the features of moyamoya-like vasculopathy. Additionally, we found that both MMD patient and Neo1 mutant mice exhibited altered gene expression in their cortex in proteins critical for not only angiogenesis [e.g., an increase in vascular endothelial growth factor (VEGFa)], but also axon guidance (e.g., netrin family proteins) and inflammation. In aggregates, these results suggest a critical role of astrocytic NEO1-loss in the development of Moyamoya-like vasculopathy, providing a mouse model for investigating mechanisms of Moyamoya-like vasculopathy.

Abstract Background Vacuolar sorting protein 35 (VPS35), a critical component of retromer, is essential for selective endosome-to-Golgi retrieval of membrane proteins. It is highly expressed in microglial cells, in addition to neurons. We have previously demonstrated microglial VPS35’s functions in preventing hippocampal, but not cortical, microglial activation, and in promoting adult hippocampal neurogenesis. However, microglial VPS35’s role in the cortex in response to ischemic stroke remains largely unclear. Methods We used mice with VPS35 cKO (conditional knockout) in microglial cells and examined and compared their responses to ischemic stroke with control mice. The brain damage, cell death, changes in glial cells and gene expression, and sensorimotor deficits were assessed by a combination of immunohistochemical and immunofluorescence staining, RT-PCR, Western blot, and neurological functional behavior tests. Results We found that microglial VPS35 loss results in an increase of anti-inflammatory microglia in mouse cortex after ischemic stroke. The ischemic stroke-induced brain injury phenotypes, including brain damage, neuronal death, and sensorimotor deficits, were all attenuated by microglial VPS35-deficiency. Further analysis of protein expression changes revealed a reduction in CX3CR1 (CX3C chemokine receptor 1) in microglial VPS35-deficient cortex after ischemic stroke, implicating CX3CR1 as a potential cargo of VPS35 in this event. Conclusion Together, these results reveal an unrecognized function of microglial VPS35 in enhancing ischemic brain injury-induced inflammatory microglia, but suppressing the injury-induced anti-inflammatory microglia. Consequently, microglial VPS35 cKO mice exhibit attenuation of ischemic brain injury response.

Background Malignant pleural effusion (MPE) predicts advanced disease and poor prognosis, and is usually diagnosed by medical thoracoscopy. It remains unclear whether the various representations visualized on thoracoscopy are reliable prognostic factors. The aim of this study was to evaluate prognostic factors for survival in patients who underwent thoracoscopy for MPE. Methods The medical records of consecutive patients with MPE who underwent medical thoracoscopy from 2007 to 2015 at a tertiary hospital were reviewed and theKaplan‐Meier method and Cox regression analysis used to determine prognostic factors. Results A total of 125 patients with non‐small cell lung cancer (NSCLC) were confirmed on tissue biopsy as having pleural metastasis. In NSCLC, factors adversely affecting overall survival (OS) in univariate analysis included extent of pleural carcinomatosis (EPC) score (P = 0.031), grade of adhesions (P = 0.037), costoparietal pleural lesions (P = 0.035) and bloody MPE (P = 0.023); Cox multivariate analysis revealed that EPC score (P = 0.007) and grade of adhesions (P = 0.019) were independent predictors of OS. Conclusions Under traditional chemotherapy, higher EPC score and higher grades of adhesions predicted poor prognosis in advanced NSCLC patients with pleural metastasis. Taking into account these factors may allow doctors to make more accurate predictions and provide individual therapy when treating patients with MPE.

NaoShuanTong capsule (NSTC) has been commonly used to treat blood circulation disorders and neurological dysfunction against cerebral ischemia in China. In this study, using an acute blood stasis rat model, canonical correlation analysis between chemical high performance liquid chromatography (HPLC) fingerprint and in vivo biotests was conducted to discover and identify the bioactive compounds responsible for NSTC’s protective effects on hemorheological disorders. Four independent effect components were extracted from the 12 originally detected parameters. Three bioactive compounds were found to have significant contribution in two or more effect components against hemorheological disorders. Typhaneoside could influence the erythrocyte aggregation and coagulation function. Isorhamnetin-3-O-neohesperidoside had impacts on the erythrocyte aggregation, erythrocyte deformability and platelet aggregation. Benzoyl-oxypaeoniflorin was associated with erythrocyte aggregation, coagulation function and platelet aggregation. The findings could offer useful references for NSTC’s quality control and clinical practice. Abbreviations Adradrenaline ASPaspirin APTTactivated partial thromboplastin time CCAcanonical correlation analysis EAIerythrocyte aggregation index EDIerythrocyte deformability index EEIerythrocyte electrophoresis index HCThaematokrit HPLChigh performance liquid chromatography MPARmaximum platelet aggregation rate NSTCNaoShuanTong Capsule PCAprincipal component analysis PTPollen Typhae PTprothrombin time PVplasma viscosity RCRadix Curcumae RGRhizome Gastrodiae RPRRadix Paeoniae Rubra RRRadix Rhapontici TCMtraditional Chinese medicine WBVwhole blood viscosit

Objective This study was performed to explore the characteristics and outcomes of patients with sepsis accompanied by active cancer who were admitted to the intensive care unit (ICU). Methods The baseline characteristics, infection profiles, and outcomes of patients with sepsis were retrospectively analyzed according to the presence of concomitant active cancer. The association between concomitant active cancer and 28-day mortality was explored. Results Of 23,956 patients with sepsis, 1574 (6.6%) had concomitant active cancer. The most common type was digestive (30.7%). The 28-day mortality ranged from 41.9% to 81.5%. Patients with active cancer had a significantly higher Simplified Acute Physiology Score II and significantly shorter length of ICU stay. Respiratory (32.9%), genitourinary (31.0%), and bloodstream (17.0%) infections were most common. Escherichia coli was the most frequent gram-negative pathogenic bacteria. The 28-day mortality rate was significantly higher in patients with than without active cancer. Concomitant active cancer was associated with increased 28-day mortality in patients with sepsis. Hematological malignancy was associated with a significantly higher risk of death than solid tumors. Conclusions Concomitant active cancer was associated with higher 28-day mortality in patients with sepsis requiring ICU admission. Hematological malignancy was associated with a higher risk of death than solid tumors.

Objective Hyperbaric oxygen (HBO) is an emerging complementary alternative medical approach in glioma treatment. However, its mode of action is unknown, so this was investigated in the present study. Methods We constructed an intracranial glioma model of congenic C57BL/6J mice. Glioma growth under HBO stimulation was assessed by bioluminescent imaging and magnetic resonance imaging. Flow cytometry assessed direct effects of HBO on reactive oxygen species (ROS) signaling of transplanted glioma cells and organs, and quantified mature T cells and subgroups in tumors, the brain, and blood. Results HBO promoted the growth of transplanted GL261-Luc glioma in the intracranial glioma mouse model. ROS signaling of glioma cells and brain cells was significantly downregulated under HBO stimulation, but thymus ROS levels were significantly upregulated. CD3+ T cells were significantly downregulated, while both Ti/Th cells (CD3+CD4+) and Ts/Tc cells (CD3+CD8+) were inhibited in tumors of the HBO group. The percentage of regulatory T cells in Ti/Th (CD3+CD4+) cells was elevated in the tumors and thymuses of the HBO group. Conclusion HBO induced ROS signaling in the thymus, inhibited CD3+ T cell generation, and facilitated malignant glioma cell growth in vivo in the intracranial glioma mouse model.

The observation of the bright pulses and dark square pulses in a graphene-oxide saturable absorber (GOSA) passively mode-locked ytterbium-doped fiber laser has been investigated experimentally. Bright pulses are achieved at a pump power of ~ 200 mW. However, the dark-square-pulse generation starts at a much higher pump power of ~ 450 mW. At the maximum pump power of 600 mW, the dark-square-pulse bunches and harmonic mode locking (HML) can be also obtained by tuning the polarization controller (PC) to different orientations. It is the first demonstration of the bunches and HML of dark square pulses in a GOSA passively mode-locked ytterbium-doped fiber laser with large normal dispersion cavity.

Objective:To evaluate the short-term outcomes of video-assisted thoracic surgery (VATS) for thoracic tumors.Methods:The data of 1,790 consecutive patients were retrospectively reviewed. These patients underwent VATS pulmonary resections, VATS esophagectomies, and VATS resections of mediastinal tumors or biopsies at the Cancer Institute & Hospital, Chinese Academy of Medical Sciences between January 2009 and January 2012.Results:There were 33 patients converted to open thoracotomy (OT, 1.84%). The overall morbidity and mortality rate was 2.79% (50/1790) and 0.28% (5/1790), respectively. The overall hospitalization and chest tube duration were shorter in the VATS lobectomy group (n=949) than in the open thoracotomy (OT) lobectomy group (n=753). There were no significant differences in morbidity rate, mortality rate and operation time between the two groups. In the esophageal cancer patients, no significant difference was found in the number of nodal dissection, chest tube duration, morbidity rate, mortality rate, and hospital length of stay between the VATS esophagectomy group (n=81) and open esophagectomy group (n=81). However, the operation time was longer in the VATS esophagectomy group. In the thymoma patients, there was no significant difference in the chest tube duration, morbidity rate, mortality rate, and hospital length of stay between the VATS thymectomy group (n=41) and open thymectomy group (n=41). However, the operation time was longer in the VATS group. The median tumor size in the VATS thymectomy group was comparable with that in the OT group.Conclusions:In early-stage (I/Ⅱ) non-small cell lung cancer patients who underwent lobectomies, VATS is comparable with the OT approach with similar short-term outcomes. In patients with resectable esophageal cancer, VATS esophagectomy is comparable with OT esophagectomy with similar morbidity and mortality. VATS thymectomy for Masaoka stage I and Ⅱ thymoma is feasible and safe, and tumor size is not contraindicated. Longer follow-ups are needed to determine the oncologic equivalency of VATS lobectomy, esophagectomy, and thymectomy for thymoma vs. OT.

We demonstrate a compact wavelength-tunable and -switchable mode-locked Yb-doped fiber (YDF) laser based on single-walled carbon nanotube (SWCNT) wall paper absorber. Two mechanisms coexist in the fiber cavity. The switchable mode-locked state can be obtained between two wavelengths depending on the fiber-loop-induced cavity birefringence. Because of the intensity-dependent transmission distribution, the proposed fiber laser can be operated in the tunable wavelength mode-locked state from 1025 to 1037 nm. The spectral bandwidth varied from 1.1 to 2.4 nm depending on the operating wavelength and the pump power with pulse duration of hundreds of picoseconds. The average wavelength spacing is 3.6 nm, which can be changed, corresponding to the birefringence of the fiber cavity. Moreover, stable wavelength-tunable mode locking is obtained at room temperature.

Although much is known about the operation of signalized intersections, little or no empirical research has been conducted regarding bicycle capacity at these locations and the correspondent contributory factors. The purpose of this study is to accurately quantify the capacity of bikeway at signalized intersection through a fluid dispersion approach, and ultimately the lane group capacity. Using this total dispersion of bicycle flow, a relationship is also described between bicycle volume per hour and per unit width, signal parameters (length of signal cycle and green time), bicycle flow (arrival rate, density, moving velocity) and geometric intersection distance. Through the videotaping of four intersections that have significant bicycle traffic around Xiaozhai in Xi’an, China, it is ascertained that bicycle capacity varies linearly (but limited by an asymptote domain) associated with the adjustment of these parameters. The analytical results indicate that the impact saturation flow of lane groups containing right-turning vehicles and pedestrian flow at signalized intersections on bicycles is being underestimated. If this is the case, then capacity is being overestimated through the HCM 2000 capacity model and JJ37-90 approach and intersections are not being adequately designed, due to the neglect of conflict nature of mixed traffic arrivals in competing for space.

There is a need to evaluate the safety performance of road intersections in a vehicle platoon environment. For this purpose, this study attempts to quantify intersection related accidents and develop countermeasures to reduce injuries resulting from such accidents, using the selected data of 3520 casualties over the period 1992–2007 in Harbin. Then the distribution characteristics of accident number and rate via a half experience model are statisticed and find that proportionately 89.15% of total records occurred at unsignalized intersections and the injuries and fatalities account for approximately 62.35% at irregular ones. Using techniques of linear regression, several different explanatory models are constructed to identify the factors associated with annual average daily traffic, junction numbers and volume/capacity and thus 3 stages are classified in response to diverse risk level. Moreover, we developed a pedestrian risk factor to quantity the crossing risk around intersection locations, due to pedestrian are more likely to be at-fault in accidents, and 8 roads in Harbin, including Hongqi Str., Nanzhi Rd., etc., are consequently detected with higher crosswalk risk. For safety enhancement approaches, effective measures are proposed to be a combination of facilities improvement, intelligent transportation system application, enforcement and educational programs implemented for both drivers, pedestrians and other users to reduce the overall number of crashes and provide a safer traffic environment.

Discovery and identification of three bioactive compounds affecting endothelial function in Ginkgo biloba Extract (GBE) based on chromatogram-bioactivity correlation analysis. Three portions were separated from GBE via D101 macroporous resin and then re-combined to prepare nine GBE samples. 21 compounds in GBE samples were identified through UFLC-DAD-Q-TOF-MS/MS. Correlation analysis between compounds differences and endothelin-1 (ET-1) in vivo in nine GBE samples was conducted. The analysis results indicated that three bioactive compounds had close relevance to ET-1: Kaempferol-3-O-α-l-glucoside, 3-O-{2-O-{6-O-[P-OH-trans-cinnamoyl]-β-d-glucosyl}-α-rhamnosyl} Quercetin isomers, and 3-O-{2-O-{6-O-[P-OH-trans-cinnamoyl]-β-d-glucosyl}-α-rhamnosyl} Kaempferide. The discovery of bioactive compounds could provide references for the quality control and novel pharmaceuticals development of GRE. The present work proposes a feasible chromatogram-bioactivity correlation based approach to discover the compounds and define their bioactivities for the complex multi-component systems.

This study was performed to investigate the correlation between CT perfusion with acetazolamide challenge and angiogenesis in C6 gliomas.Thirty-two male Sprague-Dawley rats were evaluated. The rats were divided randomly to four groups: eight rats with orthotopically implanted C6 gliomas at 10-days old (Group A), eight rats with gliomas at 14-days old (Group B), eight rats with gliomas at 18-days old (Group C), eight rats with orthotopically injected normal saline served as controls. CT perfusion was performed before and after administration of acetazolamide. Changes in perfusion parameters due to acetazolamide administration were calculated and analyzed.Elevated carbon dioxide partial pressure and decreased pH were found in all 32 rats post acetazolamide challenge (P

Many studies focused on the analysis of effect factors contributing to the crashes and development of crash prediction models have resulted in aggregate researches to quantify the safety effects of geometric and traffic variables and environmental concerns on the expected outcome of fatal, injury and/or property damage losses at specific locations. Crash insight regarding different locations, however, has rarely been performed. Such investigations are useful for at least two reasons. First, there is a priori need to identify high risk sites with respect to crash. Second, it is generally believed that different crash types (e.g. rear-end, angle etc.) are associated with road geometry, the environment and traffic condition, and as a result justifying the inside causes of such crashes helps with understanding and improving the specific ability to make effective countermeasures. Therefore, the objectives of this paper are to (1) demonstrate that different crash types are associated to intersections in different ways and (2) reveal that the statistics of intersection crashes may lead to greater insights considering crash occurrence and countermeasure effectiveness. This paper first divides crashes into 5 categories or types: pedestrian-involved, rear-end, head-on, angle and sideswipe crash types. Based on 3208 crashes collected on the intersections in the city of Harbin during the period of 1992–2008, distribution, overall count and the occurrence of rate features are estimated resulting in two models. The performed analysis reveals that safety improvement factors such as the presence of a signal light set, a traffic monitoring device and ITS measure have a positive association with intersection crash in different ways, suggesting that different traffic control and management aspects may be helpful in identifying specific countermeasures in the overall safety improvement project. Santrauka Daugelis tyrimų, nagrinėjančių efektyvumo veiksnius, padedančius nustatyti susidūrimų prognozavimo modelius, paskatino įvairius tyrimus įvertinti eismo aplinkos sudedamųjų dalių ir jų parametrų įtaką mirties ir (arba) turtinės žalos atvejams konkrečiose vietose. Iki šiol tai buvo retas reiškinys. Tokie tyrimai yra svarbūs bent jau dėl dviejų priežasčių. Pirma, reikia nustatyti padidintos rizikos vietas atsižvelgiant į eismo įvykį. Antra, manoma, kad skirtingos susidūrimo rūšys yra susijusios su kelio geometrija, aplinkos ir eismo sąlygomis ir kaip pasekmė, pateisinanti vidines tokių susidūrimų priežastis, padeda suprasti ir pagerinti konkrečias galimybes imtis atsakomųjų veiksmų. Todėl šio darbo tikslas—parodyti, kad skirtingos susidūrimo rūšys įvairiai susijusios su eismu sankryžose, kad susidūrimų sankryžose statistika gali lemti didesnę įžvalgą, atsižvelgiant į įvykusį eismo įvykį ir atsakomųjų priemonių veiksmingumą. Šiame darbe susidūrimai skirstomi į 5 rūšis: susidūrimai su pėsčiaisiais, įvažiavimas į galinę transporto priemonės dalį, susidūrimas priekinėmis transporto priemonės dalimis, kampinis smūgis ir šoninis smūgis. Pasiskirstymas, bendras skaičius ir susidūrimų dažnis apskaičiuojami pagal du modelius remiantis informacija, surinkta iš 3028 eismo įvykių, įvykusių 1992–2008 m. Charbino miesto (Kinija) sankryžose. Atlikta analizė parodė, kad saugaus gerinimo veiksniai, t. y. šviesoforas, eismo stebėjimo prietaisai ir t.t., turi teigiamą įtaką. Skirtingi eismo kontrolės ir valdymo aspektai gali padėti rasti konkrečias atsakomąsias priemones, įgyvendinant visą saugaus eismo gerinimo projektą. Резюме Проблема безопасности дорожного движения актуальна во всех городах мира. Не является исключением и китайский город Харбин. Авторы исследуют влияние совокупности факторов, возникающих до и после дорожно-транспортного происшествия, для оценки эффективности проекта по безопасному движению на перекрестках в упомянутом городе. Исследуются места увеличенного риска возникновения дорожно-транспортного происшествия. Также принимается во внимание, что различные типы дорожно-транспортных происшествий тесно связаны с геометрическими параметрами дороги, условиями окружающей среды, условиями самого движения и т. д. Целью исследования было показать связь и влияние различных типов дорожно-транспортных происшествий на конкретные ситуации движения на перекрестках. Это необходимо для того, чтобы понять необходимость ответных мер по обеспечению безопасности дорожного движения в потенциально опасных местах. Все дорожно-транспортные происшествия разделены на 5 типов. Далее на основании информации о 3028 дорожно-транспортных происшествий, зарегистрированных в период с 1992 по 2008 гг. на перекрестках города Харбина, представлены статистические результаты исследования. Проведенное исследование показало, что меры по увеличению безопасности дорожного движения (например, светофор, видео наблюдение за движением и т. д.) имели положительное влияние. Различные аспекты контроля за дорожным движением и управления им помогают найти ответные меры по претворению в жизнь проекта по обеспечению безопасности дорожного движения. Reikšminiai žodžiai: susidūrimas, sankryža, susidūrimų dažnis, eismo kontrolė, šviesos signalas, atsakomosios priemonės, saugus eismas Ключевые слова: дорожно-транспортное происшествие, безопасность дорожного движения, перекресток, столкновение, контроль за движением, ответные меры

The asymmetric unit of the title compound, C20H15NO2, contains one half-molecule with the central N atom and two C atoms of the benzene moiety lying on a twofold rotation axis. Weak C—H...O interactions join the molecules together into an infinite three-dimensional network.

The title compound, [Zn(C8H4O4)(C5H8N2)3], has a neutral monomeric structure in which one terephthalate dianion and three 2-ethyl-1H-imidazole ligands coordinate to the ZnII ion in a distorted tetrahedral geometry. The methyl group of one of the ethyl groups is disordered over two positions with occupancies of 0.66 (2) and 0.34 (2). In the crystal structure, molecules are linked into a three-dimensional hydrogen-bonded network by intermolecular N—H...O interactions involving the uncoordinated carboxylate O atoms.

Abstract This paper is concerned with the nonlinear free vibration of a heated micro/nano beam modeled after the nonlocal continuum elasticity theory and Euler-Bernoulli beam theory. The governing partial differential equations are derived from the Hamilton variational principle and von Kármán geometric nonlinearity, in which the effects of the nonlocality and ambient temperature are inclusive. These equations are converted into ordinary forms by employing the Kantorovich method. The solutions of nonlinear free vibration are then sought through the use of shooting method in spatial domain. Numerical results show that the proposed treatment provides excellent accuracy and convergence characteristics. The influences of the aspect ratio, nonlocal parameter and temperature rise parameter on the dimensionless radian frequency are carefully investigated. It is concluded that the nonlocal and temperature rise parameters lead to reductions of the nonlinear vibration frequency, while the influence of the nonlocal effect decreases with an increase in the aspect ratio.