Search

Your search keyword '"Yong, Hyo Jeong"' showing total 24 results
Start Over Author "Yong, Hyo Jeong"
24 results on '"Yong, Hyo Jeong"'

2. β-Cell Heterogeneity and Plasticity

Author

Yong, Hyo Jeong, Wang, Yue J., Sutovsky, Peter, Editor-in-Chief, Kmiec, Z., Series Editor, Balboula, Ahmed Z., Series Editor, Golson, Maria L., Series Editor, and Schumann, Sven, Series Editor

Published
2024
Full Text
View/download PDF

13. Is FAM19A5an adipokine? Peripheral FAM19A5in wild-type, FAM19A5knockout, and LacZknockin mice

Author

Kwak, Hoyun, Cho, Eun-Ho, Cho, Eun Bee, Lee, Yoo-Na, Shahapal, Anu, Yong, Hyo Jeong, Reyes-Alcaraz, Arfaxad, Jeong, Yongwoo, Lee, Yerim, Lee, Minhyeok, Ha, Nui, Oh, Sitaek, Lee, Jae Keun, Lee, Won Suk, Kim, Won Kyum, Yoo, Sangjin, Kwon, Soon-Gu, Hwang, Jong-Ik, and Seong, Jae Young

Abstract

FAM19A5is a novel secretory protein expressed primarily in the brain. However, a recent study reported that FAM19A5is an adipocyte-derived adipokine that regulates vascular smooth muscle function through sphingosine-1-phosphate receptor 2 (S1PR2). In our study, we investigated FAM19A5transcript and protein levels in peripheral tissues, including adipose tissues, from wild-type, FAM19A5knockout, and FAM19A5-LacZknockin mice. We found that the FAM19A5transcript levels in the central nervous system were much greater than those in any of the peripheral tissues, including adipose tissues. Furthermore, the FAM19A5protein levels in adipose and reproductive tissues were below detectable limits for Western blot analysis and enzyme-linked immunosorbent assay (ELISA). Additionally, we found that the FAM19A5protein did not interact with S1PR2 in terms of G-protein-mediated signal transduction, β-arrestin recruitment, or ligand-mediated internalization. Taken together, our findings revealed basal levels of FAM19A5transcripts and proteins in peripheral tissues, confirming its primary expression in the central nervous system and lack of significant interaction with S1PR2.

Published
2024
Full Text
View/download PDF

15. Is FAM19A5 an adipokine? Peripheral FAM19A5 in wild-type, FAM19A5 knock-out, and LacZ knock-in mice

Author

Kwak, Hoyun, primary, Cho, Eun-Ho, additional, Cho, Eun Bee, additional, Lee, Yoo-Na, additional, Shahapal, Anu, additional, Yong, Hyo Jeong, additional, Reyes-Alcaraz, Arfaxad, additional, Jeong, Yongwoo, additional, Lee, Yerim, additional, Lee, Minhyeok, additional, Ha, Nui, additional, Oh, Sitaek, additional, Lee, Jae Keun, additional, Lee, Won Suk, additional, Kim, Wonkyum, additional, Hwang, Jong-Ik, additional, and Seong, Jae Young, additional

Published
2020
Full Text
View/download PDF

16. Additional file 1 of CXCR7: a β-arrestin-biased receptor that potentiates cell migration and recruits β-arrestin2 exclusively through Gβγ subunits and GRK2

Author

Nguyen, Huong Thi, Arfaxad Reyes-Alcaraz, Yong, Hyo Jeong, Nguyen, Lan Phuong, Park, Hee-Kyung, Inoue, Asuka, Lee, Cheol Soon, Seong, Jae Young, and Hwang, Jong-Ik

Abstract

Additional File 1: Fig. S1. Ligand-stimulated real-time luciferase activities in HEK293 cells expressing different combinations of NanoBit constructs ofCXCR7 and β-arrestin2. Fig. S2. Ligand-stimulated real-time luciferase activities in HEK293 cells expressing different combinations of NanoBit constructs of β-arrestin2 with CXCR4 or CXCR3. Fig. S3. Receptor internalization assay using NanoBit constructs. (a) Cells expressing receptor-LgBiT and SmBiT-FYVE domain of EEA1 were treated with SDF-1α and the luciferase activities were measured in real-time. (b) Cells expressing receptor-SmBiT and LgBiT-CAAX sequence were used in the NanoBit assay. Fig. S4. Optimization of NanoBit construct combinations of Gb1 and GRKs. a and b showed luciferase activities in cells expressing different combinations of NanoBiT constructs. Fig. S5. Optimization of NanoBit construct combinations of receptor and GRK2. Fig. S6. Generation of cells lacking receptors using CRISPR system. (a) Genomic DNA PCR products from cells established with CRISPRCas9were cloned and sequenced. Red color designates guide RNA target regions. (b) RT-PCR products of either CXCR4 or CXCR7 were compared in wild-type and receptor KO HeLa cell clones. β-actin products were used as the control. (c) Membrane expression of exogenous receptors was not affected by deletion of CXCR4 or CXCR7. HiBiT constructs of the receptors were expressed in wild-type and receptor KO of HEK293 and HeLa cells, and the cells were applied to the HiBiT assay.

Published
2020
Full Text
View/download PDF

19. FAM19A5 Expression During Embryogenesis and in the Adult Traumatic Brain of FAM19A5-LacZ Knock-in Mice

Author

Shahapal, Anu, primary, Cho, Eun Bee, additional, Yong, Hyo Jeong, additional, Jeong, Inyoung, additional, Kwak, Hoyun, additional, Lee, Jae Keun, additional, Kim, Wonkyum, additional, Kim, Bongcheol, additional, Park, Hae-Chul, additional, Lee, Won Suk, additional, Kim, Hyun, additional, Hwang, Jong-Ik, additional, and Seong, Jae Young, additional

Published
2019
Full Text
View/download PDF

20. Spexin-Based Galanin Receptor Type 2 Agonist for Comorbid Mood Disorders and Abnormal Body Weight

Author

Yun, Seongsik, primary, Reyes-Alcaraz, Arfaxad, additional, Lee, Yoo-Na, additional, Yong, Hyo Jeong, additional, Choi, Jeewon, additional, Ham, Byung-Joo, additional, Sohn, Jong-Woo, additional, Kim, Dong-Hoon, additional, Son, Gi Hoon, additional, Kim, Hyun, additional, Kwon, Soon-Gu, additional, Kim, Dong Sik, additional, Kim, Bong Chul, additional, Hwang, Jong-Ik, additional, and Seong, Jae Young, additional

Published
2019
Full Text
View/download PDF

21. Von Hippel-Lindau regulates interleukin-32β stability in ovarian cancer cells

Author

Yong, Hyo Jeong, primary, Park, Jeong Su, additional, Lee Jeong, Ae, additional, Han, Sora, additional, Lee, Sunyi, additional, Ka, Hye In, additional, Sumiyasuren, Buyanravjkh, additional, Joo, Hyun Jeong, additional, So, Su Jeong, additional, Park, Ji Young, additional, Yoon, Do-Young, additional, Lim, Jong-Seok, additional, Lee, Myeong-Seok, additional, Lee, Hee Gu, additional, and Yang, Young, additional

Published
2017
Full Text
View/download PDF

23. Nafamostat mesilate negatively regulates the metastasis of triple-negative breast cancer cells.

Author

Mander, Sunam, You, Dong-Joo, Park, Sumi, Kim, Dong Hwi, Yong, Hyo Jeong, Kim, Dong-Sik, Ahn, Curie, Kim, Yun-Hee, Seong, Jae Young, and Hwang, Jong-Ik

Abstract

Triple-negative breast cancer (TNBC) lacking of oestrogen receptor, progesterone receptor, and epidermal growth factor receptor type 2 is a highly malignant disease which results in a poor prognosis and rare treatment options. Despite the use of conventional chemotherapy for TNBC tumours, resistance and short duration responses limit the treatment efficacy. Therefore, a need exists to develop a new chemotherapy for TNBC. The aim of this study was to examine the anti-cancer effects of nafamostat mesilate (NM), a previously known serine protease inhibitor and highly safe drug on breast cancer cells. Here, we showed that NM significantly inhibits proliferation, migration, and invasion in MDA-MB231 cells, induces G2/M phase cell-cycle arrest, and inhibits the expression of cyclin-dependent kinase 1 (CDK1). Exposure of MDA-MB231 cells to NM also resulted in decreased transcription factor activities accompanied by the regulated phosphorylation of signalling molecules and a decrease in metalloproteinases, the principal modulators of the extracellular environment during cancer progression. Especially, inhibition of TGFβ-stimulated Smad2 phosphorylation and subsequent metastasis-related gene expression, and downregulation of ERK activity may be pivotal mechanisms underlying inhibitory effects of NM on NM inhibits lung metastasis of breast cancer cells and growth of colonized tumours in mice. Taken together, our data revealed that NM inhibits cell growth and metastasis of TNBC cells and indicated that NM is a multi-targeted drug that could be an adjunct therapy for TNBC treatment. [ABSTRACT FROM AUTHOR]

Published
2018
Full Text
View/download PDF

Catalog

Books, media, physical & digital resources
See catalog results