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4. Another advantage of multi-locus variable-number tandem repeat analysis that can putatively subdivide enterohemorrhagic Escherichia coli O157 strains into clades by maximum a posteriori estimation

Author

Hirai, Shinichiro, primary, Yokoyama, Eiji, additional, Ando, Naoshi, additional, Seto, Junji, additional, Hazama, Kyoko, additional, Enomoto, Keigo, additional, Izumiya, Hidemasa, additional, Akeda, Yukihiro, additional, and Ohnishi, Makoto, additional

Published
2023
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5. Alteration of a Shiga toxin-encoding phage associated with a change in toxin production level and disease severity in Escherichia coli

Author

Miyata, Tatsuya, primary, Taniguchi, Itsuki, additional, Nakamura, Keiji, additional, Gotoh, Yasuhiro, additional, Yoshimura, Dai, additional, Itoh, Takehiko, additional, Hirai, Shinichiro, additional, Yokoyama, Eiji, additional, Ohnishi, Makoto, additional, Iyoda, Sunao, additional, Ogura, Yoshitoshi, additional, and Hayashi, Tetsuya, additional

Published
2023
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8. Whole-genome sequence analysis of Shiga toxin-producing Escherichia coli O157 strains isolated from wild deer and boar in Japan

Author

MORITA, Satoshi, SATO, Shingo, MARUYAMA, Soichi, NAGASAKA, Mariko, MURAKAMI, Kou, INADA, Kazuya, UCHIUMI, Masako, YOKOYAMA, Eiji, ASAKURA, Hiroshi, SUGIYAMA, Hiromu, TAKAI, Shinji, MAEDA, Ken, and KABEYA, Hidenori

Subjects
Swine Diseases, endocrine system, shiga toxin-producing Escherichia coli(STEC), Full Paper, Shiga-Toxigenic Escherichia coli, urogenital system, Swine, Deer, Escherichia coli Proteins, boar, Cattle Diseases, Membrane Transport Proteins, Animals, Wild, Japan, game meat, Animals, Cattle, Public Health, Sequence Analysis, Escherichia coli Infections
Abstract

The prevalence of Shiga toxin-producing Escherichia coli O157 (STEC O157) strains in wild deer and boar in Japan was investigated. STEC O157 strains were isolated from 1.9% (9/474) of the wild deer and 0.7% (3/426) of the wild boar examined. Pulsed-field gel electrophoresis (PFGE) analysis classified the wild deer and boar strains into five and three PFGE patterns, respectively. The PFGE pattern of one wild boar strain was similar to that of a cattle strain that had been isolated from a farm in the same area the wild boar was caught, suggesting that a STEC O157 strain may have been transmitted between wild boar and cattle. Clade analysis indicated that, although most of the strains were classified in clade 12, two strains were classified in clade 7. Whole-genome sequence (WGS) analysis indicated that all the strains carried mdfA, a drug resistance gene for macrolide antibiotics, and also pathogenicity-related genes similar to those in the Sakai strain. In conclusion, our study emphasized the importance of food hygiene in processing meat from Japanese wild animals for human consumption.

Published
2021

10. Emergence of Salmonella enterica subsp. enterica serovar Chester in a rural area of Japan

Author

AOKI, Yoshihiro, WATANABE, Yudai, KITAZAWA, Katsuhiko, ANDO, Naoshi, HIRAI, Shinichiro, and YOKOYAMA, Eiji

Subjects
Male, molecular epidemiology, Salmonella infection, Disease Outbreaks, Feces, Dogs, Japan, Salmonella, Animals, Humans, Dog Diseases, bacteremia, Serotyping, Child, Aged, Retrospective Studies, Salmonella Infections, Animal, Infant, Newborn, Infant, emerging infectious disease, Note, Electrophoresis, Gel, Pulsed-Field, Child, Preschool, dog, Salmonella Infections, Female, Public Health
Abstract

In Japan, only one outbreak of Salmonella enterica subsp. enterica serovar Chester (S. Chester) has been confirmed in 1999. We performed a single-center retrospective case review of S. Chester infections that occurred in a rural area of Japan in 2016 (n=8). Case 5 and 6 occurred in twin infants who had contact with a pet dog. The dog's stool culture was positive for S. Chester. Pulsed-field gel electrophoresis and cluster analysis of S. Chester strains revealed that all the isolates appeared to be derived from the same genetic clone. Emergence of Salmonella infection can be overlooked if cases are not reported to health authorities; therefore, core hospitals should play a role to alert the occurrence of public health issue.

Published
2020

12. The global population structure and evolutionary history of the acquisition of major virulence factor-encoding genetic elements in Shiga toxin-producing Escherichia coli O121:H19

Author

Nishida, Ruriko, primary, Nakamura, Keiji, additional, Taniguchi, Itsuki, additional, Murase, Kazunori, additional, Ooka, Tadasuke, additional, Ogura, Yoshitoshi, additional, Gotoh, Yasuhiro, additional, Itoh, Takehiko, additional, Toyoda, Atsushi, additional, Mainil, Jacques Georges, additional, Piérard, Denis, additional, Seto, Kazuko, additional, Harada, Tetsuya, additional, Isobe, Junko, additional, Kimata, Keiko, additional, Etoh, Yoshiki, additional, Hamasaki, Mitsuhiro, additional, Narimatsu, Hiroshi, additional, Yatsuyanagi, Jun, additional, Kameyama, Mitsuhiro, additional, Matsumoto, Yuko, additional, Nagai, Yuhki, additional, Kawase, Jun, additional, Yokoyama, Eiji, additional, Ishikawa, Kazuhiko, additional, Shiomoto, Takayuki, additional, Lee, Kenichi, additional, Kang, Dongchon, additional, Akashi, Koichi, additional, Ohnishi, Makoto, additional, Iyoda, Sunao, additional, and Hayashi, Tetsuya, additional

Published
2021
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18. Whole Genome Analysis Detects the Emergence of a Single Salmonella enterica Serovar Chester Clone in Japan’s Kanto Region

Author

Ando, Naoshi, primary, Sekizuka, Tsuyoshi, additional, Yokoyama, Eiji, additional, Aihara, Yoshiyuki, additional, Konishi, Noriko, additional, Matsumoto, Yuko, additional, Ishida, Kumiko, additional, Nagasawa, Koo, additional, Jourdan-Da Silva, Nathalie, additional, Suzuki, Motoi, additional, Kimura, Hirokazu, additional, Le Hello, Simon, additional, Murakami, Koichi, additional, Kuroda, Makoto, additional, Hirai, Shinichiro, additional, and Fukaya, Setsuko, additional

Published
2021
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19. Actinomyces denticolens as a causative agent of actinomycosis in animals

Author

MURAKAMI, Satoshi, KOBAYASHI, Tomoko, SEKIGAWA, Yuriko, TORII, Yasushi, KANESAKI, Yu, ISHIGE, Taichiro, YOKOYAMA, Eiji, ISHIWATA, Hiroyuki, HAMADA, Moriyuki, and TAMURA, Tomohiko

Subjects
Swine Diseases, Full Paper, Swine, Palatine Tonsil, Actinomyces denticolens, Bacteriology, RNA, Fungal, Actinomycosis, Molecular Typing, “Actinomyces suis” Franke 1973, RNA, Ribosomal, 16S, Actinomyces, Animals, nomenclature, Horse Diseases, Horses
Abstract

The name “Actinomyces suis” was applied to each actinomycete isolate from swine actinomycosis by Grässer in 1962 and Franke in 1973. Nevertheless, this specific species was not included in the “Approved List of Bacterial Name” due to absence of the type cultures. Therefore, “Actinomyces suis” based on the description of Franke 1973 has been considered as “species incertae sedis”. We isolated a number of Actinomyces strains from swine. The representative strains of them was designated as Chiba 101 that was closely similar to the description in “Actinomyces suis” reported by Franke in 1973. Interestingly, it was found that the biological characteristics of these strains were also very similar to those of Actinomyces denticolens. Furthermore, the average nucleotide identity (ANI) value between strain Chiba 101 and the type-strain of Actinomyces denticolens (=DSM 20671T) was found to be 99.95%. Sequences of the housekeeping genes and 16S rRNA gene showed 100% homology. These results strongly suggested that “Actinomyces suis” Franke 1973 is the same species as Actinomyces denticolens. Since actinomycosis caused by Actinomyces denticolens have been demonstrated in horses recently, it is necessary to recognize that Actinomyces denticolens is the pathogenic actinomycetes in broader range of animals.

Published
2018

29. Characterization of Shiga toxin-producing Escherichia coli from feces of sika deer (Cervus nippon) in Japan using PCR binary typing analysis to evaluate their potential human pathogenicity

Author

KABEYA, Hidenori, SATO, Shingo, ODA, Shinya, KAWAMURA, Megumi, NAGASAKA, Mariko, KURANAGA, Masanari, YOKOYAMA, Eiji, HIRAI, Shinichiro, IGUCHI, Atsushi, ISHIHARA, Tomoe, KUROKI, Toshiro, MORITA-ISHIHARA, Tomoko, IYODA, Sunao, TERAJIMA, Jun, OHNISHI, Makoto, and MARUYAMA, Soichi

Subjects
Genetic Markers, Full Paper, Shiga-Toxigenic Escherichia coli, Shiga toxin-producing Escherichia coli (STEC), animal diseases, Deer, biochemical phenomena, metabolism, and nutrition, bacterial infections and mycoses, Polymerase Chain Reaction, wild animal, Feces, fluids and secretions, Japan, Genes, Bacterial, bacteria, Animals, Humans, Public Health, Serotyping
Abstract

This study examined the potential pathogenicity of Shiga toxin-producing Escherichia coli (STEC) in feces of sika deer by PCR binary typing (P-BIT), using 24 selected STEC genes. A total of 31 STEC strains derived from sika deer in 6 prefectures of Japan were O-serotyped and found to be O93 (n=12), O146 (n=5), O176 (n=3), O130 (n=3), O5 (n=2), O7 (n=1), O96 (n=1), O116 (n=1), O141 (n=1), O157 (n=1) and O-untypable (n=1). Of the 31 STEC strains, 13 carried both stx1 and stx2, 5 carried only stx1, and 13 carried one or two variants of stx2. However, no Stx2 production was observed in 3 strains that carried only stx2: the other 28 strains produced the appropriate Stx. P-BIT analysis showed that the 5 O5 strains from two wild deer formed a cluster with human STEC strains, suggesting that the profiles of the presence of the 24 P-BIT genes in the deer strains were significantly similar to those in human strains. All of the other non-O157 STEC strains in this study were classified with strains from food, domestic animals and humans in another cluster. Good sanitary conditions should be used for deer meat processing to avoid STEC contamination, because STEC is prevalent in deer and deer may be a potential source of STEC causing human infections.

Published
2017

35. Genetic characteristics of emerging Salmonella enterica serovar Agona strains isolated from humans in the prior period to occurrence of the serovar shift in broilers

Author

TORII, Yasushi, primary, YOKOYAMA, Eiji, additional, SEKI, Misaki, additional, SHIGEMURA, Hiroaki, additional, ISHIGE, Taichiro, additional, YANAGIMOTO, Keita, additional, UEMATSU, Kosei, additional, ANDO, Naoshi, additional, FUJIMAKI, Tsutomu, additional, and MURAKAMI, Satoshi, additional

Published
2019
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36. Early detection of prostate cancer: results of a prostate specific antigen-based detection program in Japan

Author

Egawa, Shin, Suyama, Kazuho, Ohori, Makoto, Kawakami, Tatuhiro, Kuwao, Sadahito, Hirokado, Kazutaka, Hirano, Seiichiro, Yokoyama, Eiji, Uchida, Toyoaki, and Koshiba, Ken

Subjects
Prostate cancer -- Diagnosis, Prostate-specific antigen -- Measurement, Precancerous conditions -- Analysis, Health
Abstract

Background. Prostate cancer increasingly is becoming a medical problem in Japan. To maximize the chance for cure, extensive effort is being made to detect prostate cancer while confined to the gland.Methods. Determination was made of serum prostate specific antigen (PSA) in the early detection of prostate cancer in an Asian male population aged 55 years or older. Recommendation for biopsy was based solely on this parameter when its value exceeded 2.0 ng/ml in IMx immunoenzymetric assay.Results. Of 1189 men, 150 (12.6%) had elevated values. The proportion of males with serum PSA greater than 4.0 ng/ml was only 3.4%. Of the 99 males who underwent ultrasound-guided biopsies, 16 cancers were detected, a cancer detection rate of 1.3%. Most of these (81.3%) were clinically localized cancers. Seven patients with cancer had serum PSA levels below 4.0 ng/ml including 5 less than 3.0 ng/ml. These tumors would have been overlooked if the conventional cutoff levels of 4.0 ng/ml had been applied. Radical prostatectomy was conducted on 13 patients. All had histologic features of clinically significant cancer and 69.2% were pathologically confined within the gland.Conclusions. Elderly Asian males appear to have lower serum PSA than their Western counterparts. The optimal cutoff of serum PSA for early detection should be examined further in Asian male populations. Differences in the incidence of prostate cancer between ethnic groups may have been overestimated in previous studies owing in part to unawareness by the physician and general public. Though the frequent use of more sophisticated diagnostic modalities will likely disclose greater numbers of prostate cancers in Japan, whether early detection reduces mortality by minimizing risk of death from cancer remains a point to be determined. Cancer 1995; 76:463-72.

Published
1995

38. A disturbance suppression control system for car-mounted and portable optical disk drives

Author

Yokoyama, Eiji, Nagasawa, Masato, and Katayama, Tsuyoshi

Subjects
Magneto-optical disk drives -- Research, Control systems -- Research, Acceleration (Mechanics) -- Analysis, Business, Electronics and electrical industries, Engineering and manufacturing industries
Abstract

This paper presents a new optical drive system incorporating acceleration disturbance suppression control in the focus actuator. The new system uses a disturbance observer based on modern control theory to provide a wide range of detection. As a result, it has achieved a significant improvement in control robustness from conventional systems.

Published
1994

39. A digital VCR deck mechanism using new precision actuators for high density recording

Author

Mori, Kazuo, Tomita, Masami, Shige-eda, Tetsuya, Kokubo, Kazuyuki, Nakagawa, Kunihiko, Nagasawa, Masato, and Yokoyama, Eiji

Subjects
Video tape recorders and recording -- Research, Actuators -- Research, High-density storage -- Research, Business, Electronics and electrical industries, Engineering and manufacturing industries
Abstract

A VCR deck mechanism using high-performance actuators has been developed for high density recording and has realized a track density of 4200 TPI. Concurrently, a new type of tape transport adjustment method has been developed for the VCR deck using thin metal evaporated (ME) tape.

Published
1993

40. Precision motor control system for VCR using disturbance and velocity observer

Author

Nagasawa, Masato, Yokoyama, Eiji, and Ishida, Yoshinobu

Subjects
Video tape recorders and recording -- Research, Control theory -- Analysis, Control theory -- Research, Vibration (Aeronautics) -- Analysis, Business, Electronics and electrical industries, Engineering and manufacturing industries
Abstract

This paper describes new control technology for a VCR motor employing acceleration controller using disturbance and velocity observers based on modern control theory. The results show that this produces a robust system with lower wow - flutter.

Published
1992

44. Prevalence and genetic properties of Salmonella enterica serovar Typhimurium definitive phage type 104 isolated from Rattus norvegicus and Rattus rattus house rats in Yokohama City, Japan

Author

Yokoyama, Eiji, Maruyama, Soichi, Kabeya, Hidenori, Hara, Siro, Sata, Shin, Kuroki, Toshiro, and Yamamoto, Tomoko

Subjects
Nucleotide sequence -- Research, Drug resistance in microorganisms -- Research, Polymerase chain reaction -- Analysis, Salmonella enteritidis -- Genetic aspects, Salmonella enteritidis -- Research, Biological sciences
Abstract

The prevalence and genetic properties of Salmonella enterica serovar Typhimurium definitive phage type 104 (DT104) strains among serovar Typhimurium isolates from house rats are investigated by studying antimicrobial resistance, detection of class I integrons and antimicrobial resistance genes. The results have shown that DT104 strains are dominant in house rat colonies in each building and have suggested that house rats might play an important role in the epidemiology of DT104.

Published
2007

45. Area Control of LCD with Laser Backlight Dynamic Scanning Method

Author

Muneharu Kuwata, Hideki Yoshii, Yokoyama Eiji, Hironobu Yasui, Shuichi Kagawa, Eiji Niikura, Atsushi Michimori, Hiroaki Sugiura, and Satoru Okagaki

Subjects
Materials science, Liquid-crystal display, law, business.industry, Media Technology, Optoelectronics, Electrical and Electronic Engineering, Backlight, Laser, business, Computer Science Applications, law.invention
Published
2010

46. Whole-Genome Sequences of Two Closely Related Bacteria, Actinomyces sp. Strain Chiba101 and Actinomyces denticolens DSM 20671 T

Author

Kanesaki, Yu, primary, Ishige, Taichiro, additional, Sekigawa, Yuriko, additional, Kobayashi, Tomoko, additional, Torii, Yasushi, additional, Yokoyama, Eiji, additional, Ishiwata, Hiroyuki, additional, Hamada, Moriyuki, additional, Tamura, Tomohiko, additional, Azuma, Ryozo, additional, and Murakami, Satoshi, additional

Published
2017
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48. Structures of the mating-type loci of Cordyceps Cordyceps takaomontana

Author

Yokoyama, Eiji, Yamagishi, Kenzo, and Hara, Akira

Subjects
Reproduction -- Research, Fungi -- Physiological aspects, Microbial populations -- Environmental aspects, Microbial populations -- Physiological aspects, Microbiology -- Research, Microbiology -- Environmental aspects, Biological sciences
Abstract

The authors have investigated clavicipitaceous fungus Cordyceps takaomontana. The nucleotide sequence of C. takaomontana mating-type loci has been determined in the effort to study the molecular background of C. takaomontana mating system, and the details are presented.

Published
2003

50. Histone-like protein of Streptomyces lividans

Author

Yokoyama, Eiji, Doi, Katsumi, Kimura, Makoto, Kuhara, Satoru, and Ogata, Seiya

Subjects
Streptomyces lividans, DNA-binding protein, Hup, Histone-like protein of S. lividans (HSl)
Published
1998

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