148 results on '"Yixin Jin"'
Search Results
2. Experimental study on the performance of the dielectric barrier discharge technique in the treatment of simulated groundwater with high iron and manganese content
- Author
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Yixin Jin, Xuezhi Li, Lida Ma, Wenjie Tian, Linlin Zhang, and Aonan Liu
- Subjects
dielectric barrier discharge ,discharge power ,groundwater ,high concentrations of iron and manganese ,removal ratio ,Water supply for domestic and industrial purposes ,TD201-500 ,River, lake, and water-supply engineering (General) ,TC401-506 - Abstract
Groundwater with high concentrations of iron and manganese is harmful to the human body and modern industries. Conventional methods of treating iron and manganese have the disadvantage of high costs and complex control processes. A dielectric barrier discharge (DBD) water treatment system was built to remove iron and manganese from groundwater in this paper. Single-factor experiments were used to analyze the effect of discharge power, initial iron and manganese concentrations, gas flow rate, liquid flow rate, initial pH, flocculant and capture agent process parameters on iron and manganese removal rates. It was found that Fe2+ removal rates can reach 97.31% and Mn2+ removal rates can reach 81.42% when the initial concentrations of Fe2+ and Mn2+ are 6 and 3 mg/L, respectively. The use of 10 mg/L NaHCO3 or 5 mg/L Ca(OH)2 increased the removal rate of Fe2+ to 99% but had no effect on the removal rate of Mn2+. It was found that the optimum treatment time for Mn2+ was different for different process parameters, mainly in the range of 9–15 min, which needs to be noted in future production applications. This work shows that DBD can effectively remove iron and manganese ions from groundwater and analyzes the removal mechanism. HIGHLIGHTS The removal of iron and manganese from groundwater was investigated.; The effect of individual factors on the removal of iron and manganese was analyzed.; DBD removed up to 97.31% of Fe2+.; DBD removed up to 81.42% of Mn2+.;
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- 2023
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3. Development of STEAP1 targeting chimeric antigen receptor for adoptive cell therapy against cancer
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Yixin Jin, Kristina Berg Lorvik, Yang Jin, Carole Beck, Adam Sike, Irene Persiconi, Emilie Kvaløy, Fahri Saatcioglu, Claire Dunn, and Jon Amund Kyte
- Subjects
chimeric antigen receptor ,cell therapy ,prostate cancer ,STEAP1 ,cancer immunotherapy ,metastatic cancer mouse model ,Neoplasms. Tumors. Oncology. Including cancer and carcinogens ,RC254-282 - Abstract
Chimeric antigen receptors (CARs) that retarget T cells against CD19 show clinical efficacy against B cell malignancies. Here, we describe the development of a CAR against the six-transmembrane epithelial antigen of prostate-1 (STEAP1), which is expressed in ∼90% of prostate cancers, and subgroups of other malignancies. STEAP1 is an attractive target, as it is associated with tumor invasiveness and progression and only expressed at low levels in normal tissues, apart from the non-vital prostate gland. We identified the antibody coding sequences from a hybridoma and designed a CAR that is efficiently expressed in primary T cells. The T cells acquired the desired anti-STEAP1 specificity, with a polyfunctional response including production of multiple cytokines, proliferation, and the killing of cancer cells. The response was observed for both CD4+ and CD8+ T cells, and against all STEAP1+ target cell lines tested. We evaluated the in vivo CAR T activity in both subcutaneous and metastatic xenograft mouse models of prostate cancer. Here, the CAR T cells infiltrated tumors and significantly inhibited tumor growth and extended survival in a STEAP1-dependent manner. We conclude that the STEAP1 CAR exhibits potent in vitro and in vivo functionality and can be further developed toward potential clinical use.
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- 2022
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4. Comparative Evaluation of STEAP1 Targeting Chimeric Antigen Receptors with Different Costimulatory Domains and Spacers
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Yixin Jin, Claire Dunn, Irene Persiconi, Adam Sike, Gjertrud Skorstad, Carole Beck, and Jon Amund Kyte
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chimeric antigen receptor ,STEAP1 ,costimulatory domain ,CAR spacer ,4-1BB ,CD28 ,Biology (General) ,QH301-705.5 ,Chemistry ,QD1-999 - Abstract
We have developed a chimeric antigen receptor (CAR) against the six-transmembrane epithelial antigen of prostate-1 (STEAP1), which is expressed in prostate cancer, Ewing sarcoma, and other malignancies. In the present study, we investigated the effect of substituting costimulatory domains and spacers in this STEAP1 CAR. We cloned four CAR constructs with either CD28 or 4-1BB costimulatory domains, combined with a CD8a-spacer (sp) or a mutated IgG-spacer. The CAR T-cells were evaluated in short- and long-term in vitro T-cell assays, measuring cytokine production, tumor cell killing, and CAR T-cell expansion and phenotype. A xenograft mouse model of prostate cancer was used for in vivo comparison. All four CAR constructs conferred CD4+ and CD8+ T cells with STEAP1-specific functionality. A CD8sp_41BBz construct and an IgGsp_CD28z construct were selected for a more extensive comparison. The IgGsp_CD28z CAR gave stronger cytokine responses and killing in overnight caspase assays. However, the 41BB-containing CAR mediated more killing (IncuCyte) over one week. Upon six repeated stimulations, the CD8sp_41BBz CAR T cells showed superior expansion and lower expression of exhaustion markers (PD1, LAG3, TIGIT, TIM3, and CD25). In vivo, both the CAR T variants had comparable anti-tumor activity, but persisting CAR T-cells in tumors were only detected for the 41BBz variant. In conclusion, the CD8sp_41BBz STEAP1 CAR T cells had superior expansion and survival in vitro and in vivo, compared to the IgGsp_CD28z counterpart, and a less exhausted phenotype upon repeated antigen exposure. Such persistence may be important for clinical efficacy.
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- 2024
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5. Historical building protection and modern functional renovation practice
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Yixin Jin and shen Hu
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Environmental sciences ,GE1-350 - Abstract
The article focuses on the practice of historical building protection and modern functional renovation, using Rietveld Schröder House is a case study that explores how to effectively integrate the needs of modern life while respecting historical heritage. Research on Rietveld The structural and exterior protection of the Schröder House and the functional transformation of the internal space are analyzed in detail, and the transformation effect is evaluated through questionnaires and quantitative methods. It is expected to demonstrate the balance between preservation and modernization of historic buildings, and how such renovation can enhance the functionality, aesthetic value and socio-cultural benefits of the building.
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- 2024
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6. Phenol-soluble modulin contributes to the dispersal of Staphylococcus epidermidis isolates from catheters
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Yixin Jin, Qichen Wang, Haomin Zhang, Na Zhao, Ziyu Yang, Hua Wang, Min Li, and Qian Liu
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Staphylococcus epidermidis ,catheter ,sterile body fluids ,biofilm ,infection ,Microbiology ,QR1-502 - Abstract
Staphylococcus epidermidis (S. epidermidis), a human commensal, has been implicated in invasive infection in humans due to their ability to form biofilm. It is assumed that when a biofilm is dispersed it will subsequently cause a more severe infection. The clinical significance of S. epidermidis isolated from sterile body fluid (BF) remains unclear, and might be related to dispersal from catheter-associated biofilm infection. To evaluate this relationship, we evaluated S. epidermidis isolates from catheters (CA) or BF in hospitalized patients. Sequence type 2 (ST2) is the most prevalent type isolated from infection sites. Although the specific STs were also observed in isolates from different sites, we observed that the main sequence type was ST2, followed by ST59, among all the 114 isolates from different infection sites. Interestingly, ST2 strains isolated from BF exhibited significantly thicker biofilm than those from CA. The thicker biofilm was due to the higher expression of accumulation-associated protein (aap) but not intercellular adhesion (ica) operon. Moreover, the transcription of PSMδ and PSMε were significantly increased in ST2 strains isolated from BF. Although the bacterial loads on catheters were similar infected by CA- or BF-originated strains in mouse biofilm-associated infection model, we observed a higher CFU in peri-catheter tissues infected by ST2 clones isolated from BF, suggesting that S. epidermidis with thicker biofilm formation might be able to disperse. Taken together, our data suggested that S. epidermidis originated from diverse infection sites exhibited different biofilm forming capacity. The major ST2 clone isolated from BF exhibited thicker biofilm by increasing the expression of Aap. The higher expression of PSM of these strains may contribute to bacteria dispersal from biofilm and the following bacterial spread.
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- 2022
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7. Development of a TGFβ—IL-2/15 Switch Receptor for Use in Adoptive Cell Therapy
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Carole Beck, Nicholas Paul Casey, Irene Persiconi, Neda Nejati Moharrami, Adam Sike, Yixin Jin, and Jon Amund Kyte
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chimeric antigen receptor ,immune suppression ,tumor microenvironment ,transforming growth factor β ,prostate cancer ,switch receptor ,Biology (General) ,QH301-705.5 - Abstract
Therapy employing T cells modified with chimeric antigen receptors (CARs) is effective in hematological malignancies but not yet in solid cancers. CAR T cell activity in solid tumors is limited by immunosuppressive factors, including transforming growth factor β (TGFβ). Here, we describe the development of a switch receptor (SwR), in which the extracellular domains of the TGFβ receptor are fused to the intracellular domains from the IL-2/15 receptor. We evaluated the SwR in tandem with two variants of a CAR that we have developed against STEAP1, a protein highly expressed in prostate cancer. The SwR-CAR T cell activity was assessed against a panel of STEAP1+/− prostate cancer cell lines with or without over-expression of TGFβ, or with added TGFβ, by use of flow cytometry cytokine and killing assays, Luminex cytokine profiling, cell counts, and flow cytometry phenotyping. The results showed that the SwR-CAR constructs improved the functionality of CAR T cells in TGFβ-rich environments, as measured by T cell proliferation and survival, cytokine response, and cytotoxicity. In assays with four repeated target-cell stimulations, the SwR-CAR T cells developed an activated effector memory phenotype with retained STEAP1-specific activity. In conclusion, the SwR confers CAR T cells with potent and durable in vitro functionality in TGFβ-rich environments. The SwR may be used as an add-on construct for CAR T cells or other forms of adoptive cell therapy.
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- 2023
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8. Advanced User Credit Risk Prediction Model using LightGBM, XGBoost and Tabnet with SMOTEENN.
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Chang Yu, Yixin Jin, Qianwen Xing, Ye Zhang, Shaobo Guo, and Shuchen Meng
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- 2024
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9. Fusion 3D object tracking method based on region and point cloud registration.
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Yixin Jin, Jiawei Zhang, Yinhua Liu, Wei Mo, and Hua Chen
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- 2024
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10. Real-Time Summarization of Twitter.
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Yixin Jin, Meiqi Wang, Meng Li, Wenjing Zhou, Yi Shen, and Hao Liu
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- 2024
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11. Online Learning of Multiple Tasks and Their Relationships : Testing on Spam Email Data and EEG Signals Recorded in Construction Fields.
- Author
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Yixin Jin, Wenjing Zhou, Meiqi Wang, Meng Li, Xintao Li, Tianyu Hu, and Xingyuan Bu
- Published
- 2024
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12. Potential factors affecting the success rate of indirect pulp therapy in primary molars with deep caries: a retrospective study.
- Author
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Yue Yu, Siyuan Hao, Yixin Jin, Qiong Zhang, Yan Wang, and Jing Zou
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- 2024
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13. Unsupervised Discovery of Object Landmarks as Structural Representations.
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Yuting Zhang 0001, Yijie Guo, Yixin Jin, Yijun Luo, Zhiyuan He 0003, and Honglak Lee
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- 2018
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14. Supplementary Data from Stress-Mediated Reprogramming of Prostate Cancer One-Carbon Cycle Drives Disease Progression
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Fahri Saatcioglu, Omer F. Kuzu, Yang Jin, Aykut Üren, Partha P. Banerjee, Paul S. Rennie, Ladan Fazli, Havard Emil Danielsen, Wanja Kildal, Bulent Ozpolat, Hamada M. Mokhlis, Nermin Kahraman, Nicolai Sebastian Frengen, Yixin Jin, Martina Tesikova, Marte Livgård, Ke Deng, and Nora Pällmann
- Abstract
Supplementary Materials and Methods
- Published
- 2023
15. Data from Stress-Mediated Reprogramming of Prostate Cancer One-Carbon Cycle Drives Disease Progression
- Author
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Fahri Saatcioglu, Omer F. Kuzu, Yang Jin, Aykut Üren, Partha P. Banerjee, Paul S. Rennie, Ladan Fazli, Havard Emil Danielsen, Wanja Kildal, Bulent Ozpolat, Hamada M. Mokhlis, Nermin Kahraman, Nicolai Sebastian Frengen, Yixin Jin, Martina Tesikova, Marte Livgård, Ke Deng, and Nora Pällmann
- Abstract
One-carbon (1C) metabolism has a key role in metabolic programming with both mitochondrial (m1C) and cytoplasmic (c1C) components. Here we show that activating transcription factor 4 (ATF4) exclusively activates gene expression involved in m1C, but not the c1C cycle in prostate cancer cells. This includes activation of methylenetetrahydrofolate dehydrogenase 2 (MTHFD2) expression, the central player in the m1C cycle. Consistent with the key role of m1C cycle in prostate cancer, MTHFD2 knockdown inhibited prostate cancer cell growth, prostatosphere formation, and growth of patient-derived xenograft organoids. In addition, therapeutic silencing of MTHFD2 by systemically administered nanoliposomal siRNA profoundly inhibited tumor growth in preclinical prostate cancer mouse models. Consistently, MTHFD2 expression is significantly increased in human prostate cancer, and a gene expression signature based on the m1C cycle has significant prognostic value. Furthermore, MTHFD2 expression is coordinately regulated by ATF4 and the oncoprotein c-MYC, which has been implicated in prostate cancer. These data suggest that the m1C cycle is essential for prostate cancer progression and may serve as a novel biomarker and therapeutic target.Significance:These findings demonstrate that the mitochondrial, but not cytoplasmic, one-carbon cycle has a key role in prostate cancer cell growth and survival and may serve as a biomarker and/or therapeutic target.
- Published
- 2023
16. Identification of potential molecular mechanisms and candidate drugs for radiotherapy- and chemotherapy-induced mucositis
- Author
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Siyuan Hao, Yixin Jin, Yue Yu, Jiantao Wang, Jing Zou, and Yan Wang
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Oncology - Published
- 2023
17. DIP: Distance Information Protocol for IDMaps.
- Author
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Yixin Jin, Beichuan Zhang 0001, Vasileios Pappas, Lixia Zhang 0001, and Sugih Jamin
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- 2003
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18. On the Placement of Internet Instrumentation.
- Author
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Sugih Jamin, Cheng Jin 0009, Yixin Jin, Danny Raz, Yuval Shavitt, and Lixia Zhang 0001
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- 2000
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19. An Architecture for a Global Internet Host Distance Estimation Service.
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Paul Francis, Sugih Jamin, Vern Paxson, Lixia Zhang 0001, Daniel F. Gryniewicz, and Yixin Jin
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- 1999
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20. Improved field emission characteristic of carbon nanotubes by an Ag micro-particle intermediation layer.
- Author
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Wenhui Lu, Hang Song, Yixin Jin, Haifeng Zhao, Zhiming Li, Hong Jiang, and Guoqing Miao
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- 2008
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21. Development of STEAP1 targeting chimeric antigen receptor for adoptive cell therapy against cancer
- Author
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Yixin Jin, Kristina Berg Lorvik, Yang Jin, Carole Beck, Adam Sike, Irene Persiconi, Emilie Kvaløy, Fahri Saatcioglu, Claire Dunn, and Jon Amund Kyte
- Subjects
Cancer Research ,Oncology ,Molecular Medicine ,Pharmacology (medical) - Abstract
Chimeric antigen receptors (CARs) that retarget T cells against CD19 show clinical efficacy against B cell malignancies. Here, we describe the development of a CAR against the six-transmembrane epithelial antigen of prostate-1 (STEAP1), which is expressed in ∼90% of prostate cancers, and subgroups of other malignancies. STEAP1 is an attractive target, as it is associated with tumor invasiveness and progression and only expressed at low levels in normal tissues, apart from the non-vital prostate gland. We identified the antibody coding sequences from a hybridoma and designed a CAR that is efficiently expressed in primary T cells. The T cells acquired the desired anti-STEAP1 specificity, with a polyfunctional response including production of multiple cytokines, proliferation, and the killing of cancer cells. The response was observed for both CD4
- Published
- 2021
22. Analysis of the detectivity for triple-layer heterojunction GaSb/GaInAsSb infrared detectors.
- Author
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Tian Yuan, Soo-Jin Chua, and Yixin Jin
- Published
- 2003
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23. IDMaps: a global internet host distance estimation service.
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Paul Francis, Sugih Jamin, Cheng Jin 0009, Yixin Jin, Danny Raz, Yuval Shavitt, and Lixia Zhang 0001
- Published
- 2001
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24. Effect of In content of the buffer layer on crystalline quality and electrical property of In0.82Ga0.18As/InP grown by LP-MOCVD.
- Author
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Tiemin Zhang, Guoqing Miao, Yixin Jin, Jianchun Xie, Hong Jiang, Zhiming Li, and Hang Song
- Published
- 2007
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25. Stress-mediated reprogramming of prostate cancer one-carbon cycle drives disease progression
- Author
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Marte Livgård, Bulent Ozpolat, Nora Pällmann, Partha P. Banerjee, Nicolai Sebastian Frengen, Martina Tesikova, Omer F. Kuzu, Håvard E. Danielsen, Yixin Jin, Ke Deng, Paul S. Rennie, Hamada M. Mokhlis, Nermin Kahraman, Wanja Kildal, Yang Jin, Aykut Üren, Ladan Fazli, and Fahri Saatcioglu
- Subjects
Cancer Research ,Gene knockdown ,Cell growth ,ATF4 ,Activating Transcription Factor 4 ,Biology ,medicine.disease ,Prostate cancer ,Oncology ,Gene expression ,medicine ,Cancer research ,Gene silencing ,Reprogramming - Abstract
One-carbon (1C) metabolism has a key role in metabolic programming with both mitochondrial (m1C) and cytoplasmic (c1C) components. Here we show that activating transcription factor 4 (ATF4) exclusively activates gene expression involved in m1C, but not the c1C cycle in prostate cancer cells. This includes activation of methylenetetrahydrofolate dehydrogenase 2 (MTHFD2) expression, the central player in the m1C cycle. Consistent with the key role of m1C cycle in prostate cancer, MTHFD2 knockdown inhibited prostate cancer cell growth, prostatosphere formation, and growth of patient-derived xenograft organoids. In addition, therapeutic silencing of MTHFD2 by systemically administered nanoliposomal siRNA profoundly inhibited tumor growth in preclinical prostate cancer mouse models. Consistently, MTHFD2 expression is significantly increased in human prostate cancer, and a gene expression signature based on the m1C cycle has significant prognostic value. Furthermore, MTHFD2 expression is coordinately regulated by ATF4 and the oncoprotein c-MYC, which has been implicated in prostate cancer. These data suggest that the m1C cycle is essential for prostate cancer progression and may serve as a novel biomarker and therapeutic target. Significance: These findings demonstrate that the mitochondrial, but not cytoplasmic, one-carbon cycle has a key role in prostate cancer cell growth and survival and may serve as a biomarker and/or therapeutic target.
- Published
- 2021
26. Src/FAK complex phosphorylates cardiac myosin binding protein c (cMyBP-C) in vitro and in vivo
- Author
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Jian Wang, Rui Wang, Xu Peng, Yixin Jin, Carl Tong, Ling Wang, Shenyuan L. Zhang, Yang Liu, and Marriappan Muthuchamy
- Subjects
0303 health sciences ,biology ,Integrin ,Tyrosine phosphorylation ,030204 cardiovascular system & hematology ,3. Good health ,Cell biology ,Focal adhesion ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,chemistry ,Protein kinase domain ,Myosin ,biology.protein ,Phosphorylation ,Signal transduction ,biological phenomena, cell phenomena, and immunity ,030304 developmental biology ,Proto-oncogene tyrosine-protein kinase Src - Abstract
Cardiac myosin binding protein C (cMyBP-C) is a phosphorylation-dependent force regulator and plays an important role in controlling myosin and actin dynamic interaction. Point-mutations of cMyBP-C that interfere with cMyBP-C threonine/serine phosphorylation resulted in hypertrophic cardiomyopathy and cardiac failure. However, it remains largely unknown how cMyBP-C tyrosine phosphorylation is regulated during cardiac hypertrophy and heart failure. Integrins are receptors of extracellular matrix and are the sensors of cardiac mechanical stretch. Focal adhesion kinase (FAK) plays an essential role in integrin-initiated signal transduction and regulates multiple cellular functions in various types of cells including cardiomyocytes. To identify the regulatory mechanism of cMyBP-C tyrosine phosphorylation during cardiac hypertrophy, we examined the effect of FAK on phosphorylation of cMyBP-C. Immunoprecipitation analysis showed that FAK and cMyBP-C are associated within the intact mouse heart. Results from our mutagenesis experiments demonstrated that the FAK kinase domain was required for FAK to associate with cMyBP-C. Our data also documented that the FAK Y397 site is required for FAK and cMyBP-C association. Importantly, overexpression dominant active Src Y527F with FAK significantly enhanced cMyBP-C phosphorylation. Interestingly, overexpression of cMyBP-C inhibited FAK phosphorylation. Taken together, cMyBP-C is one of effectors of Src/FAK complex in cardiomyocyte.
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- 2020
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27. Cdc42 is required for lymphatic branching, maturation and valve formation during embryonic development
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Hongjiang Si, Jian Wang, Shenyuang L. Zhang, Yixin Jin, Xu Peng, Rui Wang, Hailin Chen, David C. Zawieja, Yang Liu, Binu Tharakan, Mariappan Muthuchamy, and Sathish Srinivasan
- Subjects
macromolecular substances ,Biology ,Actin cytoskeleton ,Cell biology ,Focal adhesion ,Endothelial stem cell ,medicine.anatomical_structure ,Vasculogenesis ,Lymphatic system ,Vascular endothelial growth factor C ,Knockout mouse ,Lymphatic vessel ,medicine ,biological phenomena, cell phenomena, and immunity - Abstract
Cdc42, a Ras-related GTPase that regulates the actin cytoskeleton, was recently shown to play an indispensable role in vasculogenesis and endothelial cell (EC) survival. Here, we determined whether Cdc42 also contributes to lymphatic development by generated two different Cdc42 knockout mice lines by crossing Cdc42/flox mice with either vascular endothelial cadherin-Cre (Cdh5α-Cre) mice or Prox1-CreERT2 mice. Our results demonstrated that depleting ECs of Cdc42 expression resulted in embryonic lethality with severe edema. Whole-mount immunofluorescence staining of both knockout embryos showed that the deletion of Cdc42 in ECs impaired lymphatic vessel branching and that the lymphatic lumen size significantly increased. Moreover, we found that the inactivation of Cdc42 compromised mesenteric collecting lymphatic vessel maturation and prevented valve formation. We go on to show that Cdc42 can be activated by vascular endothelial growth factor c (VEGFc) in cultured human dermal lymphatic ECs (HDLECs), and that knocking down Cdc42 expression in these cells using siRNA decreased VEGFc-induced focal adhesion kinase (FAK) phosphorylation. These findings, when taken together with the fact that inactivating FAK plus one allele of Cdc42 in ECs was sufficient to recapitulate the phenotypes of the Cdc42 EC knockout embryos, suggests that Cdc42 and FAK interact genetically during lymphatic development. In addition, Cdc42 and FAK regulated signal transduction is essential for blood and lymphatic vessel separation. Taken together, our data highlights the important role played by Cdc42 in the development of the lymphatic system.
- Published
- 2020
28. Land ecological environment and landscape change in western China based on sensor image classification algorithm and GIS
- Author
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Hao Shen, Yixin Jing, Jihong Dong, and Wanping Pu
- Subjects
Image classification ,Geographic information ,Land ecology ,Landscape change ,Machine learning ,Electric apparatus and materials. Electric circuits. Electric networks ,TK452-454.4 - Abstract
In order to explore the land ecological environment and landscape changes in the west, this paper builds an intelligent evaluation model based on image classification algorithms and geographic information systems. To address the challenges associated with video Geographic Information System (GIS) data retrieval, this study focuses on the organization model for such data and aims to achieve efficient and comprehensive key frame retrieval in video GIS. The research utilizes global features, local features, and deep features present in video GIS data to enhance the retrieval process and improve the quality and comprehensiveness of the retrieved results. A deep neural network is used to extract relevant features from the video GIS data, and hash coding is applied to encode the high-level features derived from the network. This approach enables efficient retrieval based on the extracted features, contributing to more accurate and relevant search results. The findings indicate that the model developed in this study has demonstrated certain practical benefits and can be effectively applied to video GIS data retrieval tasks.
- Published
- 2024
- Full Text
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29. Kinetics of Oxidation of Iodide (I–) and Hypoiodous Acid (HOI) by Peroxymonosulfate (PMS) and Formation of Iodinated Products in the PMS/I–/NOM System
- Author
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Chengchun Jiang, Yuan Gao, Lihong Wang, Juan Li, Yang Zhou, Guanqi Liu, Jun Ma, Chaoting Guan, Yixin Jin, Jin Jiang, Yi Yang, and Su-Yan Pang
- Subjects
chemistry.chemical_classification ,Ecology ,Health, Toxicology and Mutagenesis ,Inorganic chemistry ,Kinetics ,Iodide ,Monoiodoacetic Acid ,chemistry.chemical_element ,02 engineering and technology ,010501 environmental sciences ,021001 nanoscience & nanotechnology ,Iodoform ,Iodine ,01 natural sciences ,Pollution ,Natural organic matter ,Hypoiodous acid ,chemistry.chemical_compound ,Reaction rate constant ,chemistry ,Environmental Chemistry ,0210 nano-technology ,Waste Management and Disposal ,0105 earth and related environmental sciences ,Water Science and Technology - Abstract
In this work, the kinetics of transformation of iodide (I–) and hypoiodous acid (HOI) by peroxymonosulfate (PMS) and potential formation of worrisome iodinated products in the presence of natural organic matter (NOM) were investigated. As the pH increased from 5 to 10, the apparent second-order rate constants of reaction of PMS with I– gradually decreased from 1.01 × 103 to 3.86 × 102 M–1 s–1, while those for HOI increased dramatically from 1.08 × 102 to 7.90 × 104 M–1 s–1. The obtained pH-dependent rate profiles were explained well by the effects of pH-affected speciation of PMS and/or HOI. Considerable amounts of total organic iodine (TOI) could be formed in the PMS/I–/NOM system over a wide pH range. Under similar conditions, the TOI levels formed in the PMS/I–/NOM system were generally higher than those formed in the case of HOCl but much lower than those formed in the case of NH2Cl. Also, specific iodoform (IF) and monoiodoacetic acid (MIAA) were detected in both simulated and authentic waters during...
- Published
- 2017
30. Transformation of Iodide by Carbon Nanotube Activated Peroxydisulfate and Formation of Iodoorganic Compounds in the Presence of Natural Organic Matter
- Author
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Su-Yan Pang, Yixin Jin, Chengchun Jiang, Jin Jiang, Congwei Luo, Chaoting Guan, Juan Li, and Jun Ma
- Subjects
Kinetics ,Inorganic chemistry ,Iodide ,Iodates ,02 engineering and technology ,010501 environmental sciences ,01 natural sciences ,Water Purification ,chemistry.chemical_compound ,Electron transfer ,Peroxydisulfate ,Environmental Chemistry ,Humic acid ,Iodate ,0105 earth and related environmental sciences ,chemistry.chemical_classification ,Nanotubes, Carbon ,Chemistry ,food and beverages ,General Chemistry ,Iodides ,021001 nanoscience & nanotechnology ,Decomposition ,Hypoiodous acid ,0210 nano-technology ,Oxidation-Reduction ,Water Pollutants, Chemical - Abstract
In this study, we interestingly found that peroxydisulfate (PDS) could be activated by a commercial multiwalled carbon nanotube (CNT) material via a nonradical pathway. Iodide (I–) was quickly and almost completely oxidized to hypoiodous acid (HOI) in the PDS/CNT system over the pH range of 5–9, but the further transformation to iodate (IO3–) was negligible. A kinetic model was proposed, which involved the formation of reactive PDS-CNT complexes, and then their decomposition into sulfate anion (SO42–) via inner electron transfer within the complexes or by competitively reacting with I–. Several influencing factors (e.g., PDS and CNT dosages, and solution pH) on I– oxidation kinetics by this system were evaluated. Humic acid (HA) decreased the oxidation kinetics of I–, probably resulting from its inhibitory effect on the interaction between PDS and CNT to form the reactive complexes. Moreover, adsordable organic iodine compounds (AOI) as well as specific iodoform and iodoacetic acid were appreciably produc...
- Published
- 2016
31. Regulation of the unfolded protein response through ATF4 and FAM129A in prostate cancer
- Author
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Erman Akkus, Partha P. Banerjee, Nora Pällmann, Jørgen Sikkeland, Yang Jin, Paul S. Rennie, Nermin Kahraman, Bulent Ozpolat, Hatice Zeynep Nenseth, Martina Tesikova, Manohar Pradhan, Håvard E. Danielsen, Ladan Fazli, Marte Livgård, Yixin Jin, Fahri Saatcioglu, Dogukan Koc, Hamada M. Mokhlis, Aykut Üren, and Omer F. Kuzu
- Subjects
Male ,0301 basic medicine ,Cancer Research ,Biology ,medicine.disease_cause ,Mice ,03 medical and health sciences ,Prostate cancer ,0302 clinical medicine ,Biomarkers, Tumor ,Tumor Cells, Cultured ,Genetics ,medicine ,Animals ,Humans ,Gene silencing ,Molecular Biology ,Cell Proliferation ,Regulation of gene expression ,ATF4 ,Prostatic Neoplasms ,Cancer ,Endoplasmic Reticulum Stress ,medicine.disease ,Activating Transcription Factor 4 ,Neoplasm Proteins ,Gene Expression Regulation, Neoplastic ,HEK293 Cells ,030104 developmental biology ,030220 oncology & carcinogenesis ,Cancer cell ,Unfolded Protein Response ,Unfolded protein response ,Cancer research ,Carcinogenesis ,Signal Transduction - Abstract
Cancer cells exploit many of the cellular adaptive responses to support their survival needs. One such critical pathway in eukaryotic cells is the unfolded protein response (UPR) that is important in normal physiology as well as disease states, including cancer. Since UPR can serve as a lever between survival and death, regulated control of its activity is critical for tumor formation and growth although the underlying mechanisms are poorly understood. Here we show that one of the main transcriptional effectors of UPR, activating transcription factor 4 (ATF4), is essential for prostate cancer (PCa) growth and survival. Using systemic unbiased gene expression and proteomic analyses, we identified a novel direct ATF4 target gene, family with sequence similarity 129 member A (FAM129A), which is critical in mediating ATF4 effects on prostate tumorigenesis. Interestingly, FAM129A regulated both PERK and eIF2a in a feedback loop that differentially channeled the UPR output. ATF4 and FAM129A protein expression is increased in patient PCa samples compared with benign prostate. Importantly, in vivo therapeutic silencing of ATF4-FAM129A axis profoundly inhibited tumor growth in a preclinical PCa model. These data support that one of the canonical UPR branches, through ATF4 and its target gene FAM129A, is required for PCa growth and thus may serve as a novel therapeutic target.
- Published
- 2019
32. Growth of GaxIn1−xAs1−ySby alloys by metalorganic chemical vapor deposition
- Author
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Shuwei, Li, Yixin, Jin, Tianming, Zhou, Baolin, Zhang, Yongqiang, Ning, Hong, Jiang, and Guang, Yuan
- Published
- 1995
- Full Text
- View/download PDF
33. Oxidation of the odorous compound 2,4,6-trichloroanisole by UV activated persulfate: Kinetics, products, and pathways
- Author
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Jin Jiang, Su-Yan Pang, Congwei Luo, Yang Song, Juan Li, Jun Ma, Daoji Wu, Yongze Liu, Chaoting Guan, and Yixin Jin
- Subjects
Environmental Engineering ,2,4,6-Trichloroanisole ,Bicarbonate ,Inorganic chemistry ,Kinetics ,Carbonates ,02 engineering and technology ,010501 environmental sciences ,01 natural sciences ,Chloride ,chemistry.chemical_compound ,Reaction rate constant ,Benzoquinones ,medicine ,Waste Management and Disposal ,0105 earth and related environmental sciences ,Water Science and Technology ,Civil and Structural Engineering ,Sulfates ,Ecological Modeling ,Hydrogen-Ion Concentration ,021001 nanoscience & nanotechnology ,Persulfate ,Phosphate ,Pollution ,chemistry ,Yield (chemistry) ,0210 nano-technology ,Oxidation-Reduction ,Water Pollutants, Chemical ,medicine.drug - Abstract
The transformation efficiency and products of an odorous compound 2,4,6-trichloroanisole (TCA) at the wavelength of 254 nm in the presence of persulfate were investigated for the first time. The effects of water matrix (i.e., natural organic matter (NOM), pH, carbonate/bicarbonate (HCO3(-)/CO3(2-)), and chloride ions (Cl(-))) were evaluated. The second order rate constant of TCA reacting with sulfate radical (SO4(-)) was determined to be (3.72 ± 0.10) × 10(9) M(-1) s(-1). Increasing dosage of persulfate increased the observed pseudo-first-order rate constant for TCA degradation (kobs), and the contribution of SO4(-) to TCA degradation was much higher than that of HO at each experimental condition. Degradation rate of TCA decreased with pH increasing from 4.0 to 9.0, which could be explained by the lower radical scavenging effect of dihydrogen phosphate than hydrogen phosphate in acidic condition (pH
- Published
- 2016
34. Unsupervised Discovery of Object Landmarks as Structural Representations
- Author
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Yixin Jin, Yijun Luo, Yuting Zhang, Yijie Guo, Zhiyuan He, and Honglak Lee
- Subjects
FOS: Computer and information sciences ,Artificial neural network ,business.industry ,Interface (Java) ,Computer science ,Computer Vision and Pattern Recognition (cs.CV) ,Computer Science - Computer Vision and Pattern Recognition ,ComputingMethodologies_IMAGEPROCESSINGANDCOMPUTERVISION ,Learning object ,Pattern recognition ,02 engineering and technology ,Iterative reconstruction ,010501 environmental sciences ,Object (computer science) ,01 natural sciences ,Visualization ,0202 electrical engineering, electronic engineering, information engineering ,020201 artificial intelligence & image processing ,Artificial intelligence ,business ,0105 earth and related environmental sciences - Abstract
Deep neural networks can model images with rich latent representations, but they cannot naturally conceptualize structures of object categories in a human-perceptible way. This paper addresses the problem of learning object structures in an image modeling process without supervision. We propose an autoencoding formulation to discover landmarks as explicit structural representations. The encoding module outputs landmark coordinates, whose validity is ensured by constraints that reflect the necessary properties for landmarks. The decoding module takes the landmarks as a part of the learnable input representations in an end-to-end differentiable framework. Our discovered landmarks are semantically meaningful and more predictive of manually annotated landmarks than those discovered by previous methods. The coordinates of our landmarks are also complementary features to pretrained deep-neural-network representations in recognizing visual attributes. In addition, the proposed method naturally creates an unsupervised, perceptible interface to manipulate object shapes and decode images with controllable structures. The project webpage is at http://ytzhang.net/projects/lmdis-rep, Comment: 48 pages
- Published
- 2018
35. The sensitivity of the yeast, Saccharomyces cerevisiae, to acetic acid is influenced by DOM34 and RPL36A
- Author
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Cindy Leung, Jennifer Yixin Jin, Katayoun Omidi, Thet Naing, Mohsen Hooshyar, Kristina Shostak, Mohan Babu, Myron L. Smith, Sarah Shaikho, Maryam Hajikarimlou, Anna York-Lyon, Rami Megarbane, Houman Moteshareie, Daniel Burnside, Tom Kazmirchuk, Bahram Samanfar, Kama E. Szereszewski, Paula Ludovico, Martin Holcik, Imelda Galván Márquez, Ashkan Golshani, [et al.], and Universidade do Minho
- Subjects
0301 basic medicine ,Saccharomyces cerevisiae ,lcsh:Medicine ,DOM34 ,Acetic acid ,General Biochemistry, Genetics and Molecular Biology ,03 medical and health sciences ,chemistry.chemical_compound ,0302 clinical medicine ,Heat shock protein ,Protein biosynthesis ,Genetics ,HSP ,Molecular Biology ,Science & Technology ,biology ,Gene deletion ,General Neuroscience ,lcsh:R ,DOM34 and RPL36A ,Translation (biology) ,General Medicine ,Cell Biology ,biology.organism_classification ,Yeast ,3. Good health ,030104 developmental biology ,chemistry ,Biochemistry ,Heat shock ,030220 oncology & carcinogenesis ,Chaperone (protein) ,RPL36A ,biology.protein ,Protein expression ,Fermentation ,General Agricultural and Biological Sciences - Abstract
The presence of acetic acid during industrial alcohol fermentation reduces the yield of fermentation by imposing additional stress on the yeast cells. The biology of cellular responses to stress has been a subject of vigorous investigations. Although much has been learned, details of some of these responses remain poorly understood. Members of heat shock chaperone HSP proteins have been linked to acetic acid and heat shock stress responses in yeast. Both acetic acid and heat shock have been identified to trigger different cellular responses including reduction of global protein synthesis and induction of programmed cell death. Yeast HSC82 and HSP82 code for two important heat shock proteins that together account for 1-2% of total cellular proteins. Both proteins have been linked to responses to acetic acid and heat shock. In contrast to the overall rate of protein synthesis which is reduced, the expression of HSC82 and HSP82 is induced in response to acetic acid stress. In the current study we identified two yeast genes DOM34 and RPL36A that are linked to acetic acid and heat shock sensitivity. We investigated the influence of these genes on the expression of HSP proteins. Our observations suggest that Dom34 and RPL36A influence translation in a CAP-independent manner., This work was funded by the Natural Sciences and Engineering Research Council of Canada, NSERC., info:eu-repo/semantics/publishedVersion
- Published
- 2017
36. Basement membrane-related regulators for prediction of prognoses and responses to diverse therapies in hepatocellular carcinoma
- Author
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Ruili Ding, Chuanbing Zhao, Yixin Jing, Rong Chen, and Qingtao Meng
- Subjects
Hepatocellular carcinoma ,BMR ,Basement membrane ,Drug sensitivity ,Immunotherapy ,Internal medicine ,RC31-1245 ,Genetics ,QH426-470 - Abstract
Abstract Background Hepatocellular carcinoma (HCC) remains a global health threat. Finding a novel biomarker for assessing the prognosis and new therapeutic targets is vital to treating this patient population. Our study aimed to explore the contribution of basement membrane-related regulators (BMR) to prognostic assessment and therapeutic response prediction in HCC. Material and methods The RNA sequencing and clinical information of HCC were downloaded from TCGA-LIHC, ICGC-JP, GSE14520, GSE104580, and CCLE datasets. The BMR signature was created by the Least Absolute Shrinkage and Selection Operator (LASSO) algorithm and used to separate HCC patients into low- and high-risk groups. We conducted analyses using various R 4.1.3 software packages to compare prognoses and responses to immunotherapy, transcatheter arterial chemoembolization (TACE), and chemotherapeutic drugs between the groups. Additionally, stemness indices, molecular functions, and somatic mutation analyses were further explored in these subgroups. Results The BMR signature included 3 basement membrane-related genes (CTSA, P3H1, and ADAM9). We revealed that BMR signature was an independent risk contributor to poor prognosis in HCC, and high-risk group patients presented shorter overall survival. We discovered that patients in the high-risk group might be responsive to immunotherapy, while patients in the low-risk group may be susceptible to TACE therapy. Over 300 agents were screened to identify effective drugs for the two subgroups. Conclusion Overall, basement membrane-related regulators represent novel biomarkers in HCC for assessing prognosis, response to immunotherapy, the effectiveness of TACE therapy, and drug susceptibility.
- Published
- 2023
- Full Text
- View/download PDF
37. Inactivation of Cdc42 in neural crest cells causes craniofacial and cardiovascular morphogenesis defects
- Author
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Yixin Jin, Edward Seto, Yang Liu, Wei Yu, Shenyuan L. Zhang, Maria Blazo, Enoch Kuo, Jieli Li, Robert J. Schwartz, and Xu Peng
- Subjects
Male ,Genotype ,Persistent truncus arteriosus ,Cardiovascular Abnormalities ,Morphogenesis ,Bone Morphogenetic Protein 2 ,Thymus Gland ,macromolecular substances ,Biology ,Aortic arches ,Craniofacial Abnormalities ,Mice ,Cell Movement ,Osteogenesis ,Cell polarity ,medicine ,Animals ,Pseudopodia ,Cdc42 ,Craniofacial ,cdc42 GTP-Binding Protein ,GTPase ,Molecular Biology ,Crosses, Genetic ,Cytoskeleton ,Neural crest cell ,Migration ,Mice, Knockout ,Craniofacial malformation ,Neural crest ,Cell Differentiation ,Cell Biology ,Anatomy ,Embryo, Mammalian ,medicine.disease ,Embryonic stem cell ,Actins ,Cell biology ,Enzyme Activation ,Phenotype ,medicine.anatomical_structure ,Neural Crest ,embryonic structures ,Female ,Gene Deletion ,Pharyngeal arch ,Developmental Biology - Abstract
Neural crest cells (NCCs) are physically responsible for craniofacial skeleton formation, pharyngeal arch artery remodeling and cardiac outflow tract septation during vertebrate development. Cdc42 (cell division cycle 42) is a Rho family small GTP-binding protein that works as a molecular switch to regulate cytoskeleton remodeling and the establishment of cell polarity. To investigate the role of Cdc42 in NCCs during embryonic development, we deleted Cdc42 in NCCs by crossing Cdc42 flox mice with Wnt1-cre mice. We found that the inactivation of Cdc42 in NCCs caused embryonic lethality with craniofacial deformities and cardiovascular developmental defects. Specifically, Cdc42 NCC knockout embryos showed fully penetrant cleft lips and short snouts. Alcian Blue and Alizarin Red staining of the cranium exhibited an unfused nasal capsule and palatine in the mutant embryos. India ink intracardiac injection analysis displayed a spectrum of cardiovascular developmental defects, including persistent truncus arteriosus, hypomorphic pulmonary arteries, interrupted aortic arches, and right-sided aortic arches. To explore the underlying mechanisms of Cdc42 in the formation of the great blood vessels, we generated Wnt1Cre-Cdc42-Rosa26 reporter mice. By beta-galactosidase staining, a subpopulation of Cdc42-null NCCs was observed halting in their migration midway from the pharyngeal arches to the conotruncal cushions. Phalloidin staining revealed dispersed, shorter and disoriented stress fibers in Cdc42-null NCCs. Finally, we demonstrated that the inactivation of Cdc42 in NCCs impaired bone morphogenetic protein 2 (BMP2)-induced NCC cytoskeleton remodeling and migration. In summary, our results demonstrate that Cdc42 plays an essential role in NCC migration, and inactivation of Cdc42 in NCCs impairs craniofacial and cardiovascular development in mice.
- Published
- 2013
38. Comparative Research between DAS-VSP and Conventional VSP Data
- Author
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Zhidong Cai, Qinghong Zhang, Gang Yu, Haiying Zhao, Yufeng Zhao, Chen Yuanzhong, and Yixin Jin
- Subjects
010504 meteorology & atmospheric sciences ,Comparative research ,Data mining ,010502 geochemistry & geophysics ,computer.software_genre ,01 natural sciences ,computer ,Geology ,0105 earth and related environmental sciences - Published
- 2016
39. Essential role of Cdc42 in cardiomyocyte proliferation and cell-cell adhesion during heart development
- Author
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Sarah Lu, Jieli Li, Jian Wang, Yixin Jin, Xu Peng, Yang Liu, Vincent VanBuren, David E. Dostal, Shenyuan L. Zhang, and Rui Wang
- Subjects
0301 basic medicine ,Heart Septal Defects, Ventricular ,Myosin light-chain kinase ,macromolecular substances ,CDC42 ,Cell Communication ,Biology ,Sarcomere ,03 medical and health sciences ,Cell Adhesion ,Animals ,Myocytes, Cardiac ,Sarcomere organization ,Cell adhesion ,Heart formation ,cdc42 GTP-Binding Protein ,Molecular Biology ,Cells, Cultured ,beta Catenin ,Cell Proliferation ,Mice, Knockout ,Heart development ,Cell Membrane ,Gene Expression Regulation, Developmental ,Cell migration ,Heart ,Cell Biology ,Cadherins ,Embryo, Mammalian ,Molecular biology ,Cell biology ,Protein Transport ,030104 developmental biology ,Organ Specificity ,Embryo Loss ,Gene Deletion ,Developmental Biology - Abstract
Cdc42 is a member of the Rho GTPase family and functions as a molecular switch in regulating cell migration, proliferation, differentiation and survival. However, the role of Cdc42 in heart development remains largely unknown. To determine the function of Cdc42 in heart formation, we have generated a Cdc42 cardiomyocyte knockout (CCKO) mouse line by crossing Cdc42 flox mice with myosin light chain (MLC) 2a-Cre mice. The inactivation of Cdc42 in embryonic cardiomyocytes induced lethality after embryonic day 12.5. Histological analysis of CCKO embryos showed cardiac developmental defects that included thin ventricular walls and ventricular septum defects. Microarray and real-time PCR data also revealed that the expression level of p21 was significantly increased and cyclin B1 was dramatically decreased, suggesting that Cdc42 is required for cardiomyocyte proliferation. Phosphorylated Histone H3 staining confirmed that the inactivation of Cdc42 inhibited cardiomyocytes proliferation. In addition, transmission electron microscope studies showed disorganized sarcomere structure and disruption of cell-cell contact among cardiomyocytes in CCKO hearts. Accordingly, we found that the distribution of N-cadherin/β-Catenin in CCKO cardiomyocytes was impaired. Taken together, our data indicate that Cdc42 is essential for cardiomyocyte proliferation, sarcomere organization and cell-cell adhesion during heart development.
- Published
- 2016
40. Nuclear Import of Exogenous FGF1 Requires the ER-Protein LRRC59 and the Importins Kpnα1 and Kpnβ1
- Author
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Yixin Jin, Yan Zhen, Camilla Skiple Skjerpen, Antoni Wiedlocha, Ellen Margrethe Haugsten, Sébastien Wälchli, Vigdis Sørensen, and Sjur Olsnes
- Subjects
Cell Biology ,Importin ,Biology ,Biochemistry ,Cell biology ,Cytosolic part ,Structural Biology ,Ran ,Genetics ,Nucleoporin ,Nuclear transport ,Nuclear pore ,Nuclear protein ,Molecular Biology ,Nuclear localization sequence - Abstract
Fibroblast growth factor 1 (FGF1) taken up by cells into endocytic vesicles can be translocated across vesicular membranes into the cytosol and the nucleus where it has a growth regulatory activity. Previously, leucine-rich repeat containing 59 (LRRC59) was identified as an intracellular binding partner of FGF1, but its biological role remained unknown. Here, we show that LRRC59 is strictly required for nuclear import of exogenous FGF1. siRNA-mediated depletion of LRRC59 did not inhibit the translocation of FGF1 into cytosol, but blocked the nuclear import of FGF1. We also found that an nuclear localization sequence (NLS) in FGF1, Ran GTPase, karyopherin-α1 (Kpnα1), and Kpnβ1 were required for nuclear import of FGF1. Nuclear import of exogenous FGF2, which depends on CEP57/Translokin, was independent of LRRC59, but was dependent on Kpnα1 and Kpnβ1, while the nuclear import of FGF1 was independent of CEP57. LRRC59 is a membrane-anchored protein that localizes to the endoplasmic reticulum (ER) and the nuclear envelope (NE). We found that LRRC59 possesses NLS-like sequences in its cytosolic part that can mediate nuclear import of soluble LRRC59 variants, and that the localization of LRRC59 to the NE depends on Kpnβ1. We propose that LRRC59 facilitates transport of cytosolic FGF1 through nuclear pores by interaction with Kpns and movement of LRRC59 along the ER and NE membranes.
- Published
- 2012
41. Abstract 16137: Essential Role of Cdc42 in Cardiomyocyte Proliferation and Cell-cell Adhesion During Heart Development
- Author
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Jieli Li, Yang Liu, Yixin Jin, Rui Wang, Vincent VanBuren, David E Dostal, Shenyuan L Zhang, and Xu Peng
- Subjects
Physiology (medical) ,macromolecular substances ,Cardiology and Cardiovascular Medicine - Abstract
Cdc42 is a member of the Rho GTPase family and functions as a molecular switch in regulating cell migration, proliferation, differentiation and survival. However, the role of Cdc42 in heart development remains largely unknown. To determine the function of Cdc42 in heart formation, we have generated a Cdc42 cardiomyocyte knockout (CCKO) mouse line by crossing Cdc42 flox mice with myosin light chain (MLC) 2a-Cre mice. The inactivation of Cdc42 in embryonic cardiomyocytes induced lethality after embryonic day 12.5. Histological analysis of CCKO embryos showed cardiac developmental defects that included thin ventricular walls and ventricular septum defects. Microarray and real-time PCR data also revealed that the expression level of p21 was significantly increased and cyclin B1 was dramatically decreased, suggesting that Cdc42 is required for cardiomyocyte proliferation. Phosphorylated Histone H3 staining confirmed that the inactivation of Cdc42 inhibited cardiomyocytes proliferation. In addition, transmission electron microscope studies showed disorganized sarcomere structure and disruption of cell-cell contact among cardiomyocytes in CCKO hearts. Accordingly, we found that the distribution of N-cadherin/β-Catenin in CCKO cardiomyocytes was impaired. Taken together, our data indicate that Cdc42 is essential for cardiomyocyte proliferation, sarcomere organization and cell-cell adhesion during heart development.
- Published
- 2015
42. Abstract 15945: Cdc42 is Required for Lymphatic Branching, Maturation and Valve Formation During Development
- Author
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Yixin Jin, Yang Liu, Hailin Chen, Rui Wang, Hongjiang Si, Sathish Srinivasan, Shenyuan Zhang, Mariappan Muthuchamy, David Zawieja, and Xu Peng
- Subjects
Physiology (medical) ,macromolecular substances ,biological phenomena, cell phenomena, and immunity ,Cardiology and Cardiovascular Medicine - Abstract
Cdc42 is a Ras-related GTPase that plays an important role in regulation of actin cytoskeletal architecture. Blocking the ability of Cdc42 to activate its effectors has been shown to inhibit a range of cellular functions including cell polarization, migration, proliferation and differentiation. Consistent with its critical roles in vitro, the inactivation of Cdc42 in mice resulted in embryonic lethality before E6.5. The early embryonic lethal phenotype of Cdc42-null mice has made it not useful for studies on the interesting questions of the roles and mechanisms of Cdc42 in the vascular development. To overcome this problem, we have generated an endothelial cell (EC) specific Cdc42 knockout mouse line by crossing Cdc42/flox mice with vascular endothelial cadherin Cre mice. Our results have demonstrated that the deletion of Cdc42 in ECs resulted in embryonic lethality with severe edema. Whole mount immunofluorescence staining showed mesentery collecting lymphatic vessel maturation and valve formation defects. Interestingly, lymphangiogenesis in the intestine wall was totally disrupted in Cdc42 EC knockout embryos. Moreover, we analyzed the role of Cdc42 in lymphatic vessel formation in the skin. We found that Cdc42 is required for tip cells filopodia formation and the deletion of Cdc42 in endothelial cells impaired lymphatic vessel sprouting, branching and the mutant lymphatic vessels displayed blunt-ended, bulbous lymphatic. We also noted that the size of lymphatic lumen was significantly increased. Taken together, our data suggested that Cdc42 plays an essential role in lymphatic branching, maturation and valve formation during development.
- Published
- 2015
43. Probing into the effect of Auger recombination mechanism on zero bias resistance–area product in In1−xGaxAs detector
- Author
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Fubin Gao, Yuchun Chang, Jingzhi Yin, Bao Shi, Longhai Li, Guotong Du, and Yixin Jin
- Subjects
Recombination velocity ,Auger effect ,Chemistry ,Infrared ,Detector ,Analytical chemistry ,Photodetector ,General Chemistry ,Condensed Matter Physics ,symbols.namesake ,Product (mathematics) ,Materials Chemistry ,symbols ,Figure of merit ,Zero bias ,Atomic physics - Abstract
D * (Detectivity), an important figure of merit for photodetectors, is limited by zero bias resistance–area product ( R 0 A ) . R 0 A is determined by Auger recombination mechanism, depending on the composition, temperature, carrier concentration and other parameters of the photodetectors. To investigate R 0 A of In1−xGaxAs infrared photodetectors, in this paper, theoretical analysis of Auger recombination mechanism was carried out in the room temperature, by taking CCCH, CHHL and CHHS into account. The calculated results show that there are significant influences on R 0 A for various parameters in both p- and n-type regions of the devices. With carrier concentration around 1017 to 1018 cm−3, R 0 A of 108 Ω cm2 (n-region) and 106 Ω cm2 (p-region) are obtained for x = 0.47 , when thickness and surface recombination velocity of the sample are 5 μm and 100 m/s, respectively.
- Published
- 2011
44. Translocation of exogenous FGF1 into cytosol and nucleus is a periodic event independent of receptor kinase activity
- Author
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Malgorzata Zakrzewska, Antoni G Wiedlocha, Vigdis Sørensen, Sjur Olsnes, and Yixin Jin
- Subjects
Cell Nucleus ,biology ,MAP kinase kinase kinase ,Cyclin-dependent kinase 2 ,Active Transport, Cell Nucleus ,Receptor Protein-Tyrosine Kinases ,Cell Biology ,Mitogen-activated protein kinase kinase ,Receptor tyrosine kinase ,Cell biology ,Mice ,Cytosol ,COS Cells ,Chlorocebus aethiops ,NIH 3T3 Cells ,biology.protein ,Animals ,Fibroblast Growth Factor 1 ,Cyclin-dependent kinase 9 ,Receptor, Fibroblast Growth Factor, Type 1 ,Kinase activity ,Platelet-derived growth factor receptor ,Signal Transduction ,Proto-oncogene tyrosine-protein kinase Src - Abstract
Fibroblast growth factor 1 (FGF1) has the property to become translocated from the extracellular space into the cell cytosol and nucleus. Membrane translocation of FGF1 occurs subsequent to endocytic uptake and is strictly FGF-receptor (FGFR) dependent. Here we have investigated the timing of FGF1 translocation in relation to FGFR1 signalling. We found that the translocation of FGF1 is a periodic event that occurs with 24h intervals. Serum-starved cells translocated the growth factor with peak occurrences ~6 h, ~30 h, and ~54 h after the addition of FGF1. The periodic FGF1 translocation was totally independent of the FGFR1 tyrosine kinase activity as it proceeded unchanged when the kinase activity was chemically inhibited or the kinase domain was deleted. Furthermore, FGF1 translocation was not restricted to a particular phase of the cell cycle or dependent on cell cycle progression. The results demonstrate that the FGF1/FGFR1 complex constitutes a signalling module that independently of the receptor tyrosine kinase can convey a signal that initiates a strictly timed and periodic release of endocytosed FGF1 into the cytosol/nucleus.
- Published
- 2011
45. Growth and optical properties of catalyst-free InP nanowires on Si (100) substrates
- Author
-
Hang Song, Hong Jiang, Ligong Zhang, Xiaojuan Sun, Guoqing Miao, Shuzhen Yu, Yixin Jin, Zhiming Li, and Dabing Li
- Subjects
Photoluminescence ,Materials science ,business.industry ,Band gap ,Nanowire ,Chemical vapor deposition ,Condensed Matter Physics ,Atomic and Molecular Physics, and Optics ,Electronic, Optical and Magnetic Materials ,symbols.namesake ,Optics ,symbols ,Optoelectronics ,Metalorganic vapour phase epitaxy ,Vapor–liquid–solid method ,business ,Electronic band structure ,Raman scattering - Abstract
Catalyst-free InP nanowires were grown on Si (1 0 0) substrates by metal-organic chemical vapor deposition. Morphology, crystal structure, photoluminescence, and Raman scattering properties of the nanowires were investigated. Most nanowires are long and straight; the angles between the nanowires and the Si substrate are diverse. The photoluminescence peak shows blue-shift from the band gap energy of bulk InP. Both the blue-shift of photoluminescence peak and the full width at half-maximum of photoluminescence spectrum increase with decreasing nanowires growth temperature. Due to laser-induced heating, the TO and LO phonon peaks of the nanowires reveal downshift and asymmetric broadening compared with those of bulk InP at room temperature.
- Published
- 2010
46. A study of two-step growth and properties of In0.82Ga0.18As on InP
- Author
-
Hong Jiang, Tiemin Zhang, Zhiming Li, Guoqing Miao, Yixin Jin, Shuzhen Yu, and Hang Song
- Subjects
Diffraction ,Morphology (linguistics) ,Materials science ,Phonon ,Scanning electron microscope ,Mechanical Engineering ,Two step ,Analytical chemistry ,Frequency shift ,Condensed Matter Physics ,Stress (mechanics) ,Crystallography ,symbols.namesake ,Mechanics of Materials ,symbols ,General Materials Science ,Raman spectroscopy - Abstract
In 0.82 Ga 0.18 As was grown by LP-MOCVD on InP substrates with the two-step growth technique. It was analyzed that epilayer's growth temperature affected on the crystalline quality, surface morphology, carrier concentration, and mobility of the In 0.82 Ga 0.18 As, which was characterized by X-ray diffraction, scanning electron microscopy, and Hall measurements. The evaluation of stress in In 0.82 Ga 0.18 As was made from frequency shift of the GaAs-like LO phonon of the Raman spectrum.
- Published
- 2009
47. Effect of buffer thickness on properties of In0.8Ga0.2As/InP with two-step growth technique
- Author
-
Hong Jiang, Guoqing Miao, Tiemin Zhang, Yixin Jin, Shuzhen Yu, Hang Song, and Zhiming Li
- Subjects
Electron mobility ,Chemistry ,Scanning electron microscope ,Mechanical Engineering ,Metals and Alloys ,Analytical chemistry ,Substrate (electronics) ,Chemical vapor deposition ,Surface coating ,symbols.namesake ,Mechanics of Materials ,Materials Chemistry ,symbols ,Metalorganic vapour phase epitaxy ,Raman spectroscopy ,Raman scattering - Abstract
In 0.8 Ga 0.2 As was grown by low-pressure metalorganic chemical vapor deposition (LP-MOCVD) on InP(1 0 0) substrate with two-step growth technique. Effect of buffer thickness on crystalline quality, surface morphology, electrical property and stress of In 0.8 Ga 0.2 As epilayer was analyzed, and properties of the In 0.8 Ga 0.2 As epilayer were characterized by X-ray diffraction, scanning electron microscopy, Hall measurements and Raman scattering. The experiments showed that the properties of the In 0.8 Ga 0.2 As epilayer had close relation to the buffer thickness and the optimum buffer thickness was about 100 nm.
- Published
- 2009
48. Effect of buffer layer thickness and epilayer growth temperature on crystalline quality of InAs0.9Sb0.1 grown by MOCVD
- Author
-
Yixin Jin, Jianchun Xie, Tiemin Zhang, Hong Jiang, Shuzhen Yu, Hang Song, Guoqing Miao, and Zhiming Li
- Subjects
Materials science ,Scanning electron microscope ,Mechanical Engineering ,Metals and Alloys ,Chemical vapor deposition ,Layer thickness ,Buffer (optical fiber) ,Metal ,Crystallography ,Compressive strength ,Quality (physics) ,Mechanics of Materials ,visual_art ,Materials Chemistry ,visual_art.visual_art_medium ,Metalorganic vapour phase epitaxy ,Composite material - Abstract
InAs 0.9 Sb 0.1 epilayers are grown on GaAs (001) substrates by metal organic chemical vapor deposition (MOCVD). In order to relax compressive strain caused by lattice mismatch between InAs 0.9 Sb 0.1 and GaAs, we employ a two-step growth method in which low temperature (430 °C) InAs 0.9 Sb 0.1 buffer layers with different thicknesses are introduced into the structure. Effect of the buffer layer thickness and the epilayer's growth temperature on crystalline quality of the epilayer is investigated, respectively. It is clear that there are strip pyramids paralleling with each other on most surface of the samples. The crystalline quality gets well obviously when the buffer layer thickness change from 0 to 50 nm, but it gets worse when the buffer layer thickness increases to 100 nm. It is also shown that the crystalline quality of the epilayer is improved obviously when the epilayer is grown at 500 °C, and it gets worse when the growth temperature decreases or increases.
- Published
- 2008
49. Electrophoresis deposition and field emission characteristics of planar-gate-type electron source with carbon nanotubes
- Author
-
Guoqing Miao, Yixin Jin, Wenhui Lu, Hui zhao, Haifeng Zhao, Zhiming Li, Lianzhen Cao, Hang Song, and Hong Jiang
- Subjects
Working electrode ,Materials science ,business.industry ,Nanotechnology ,Carbon nanotube ,Condensed Matter Physics ,Cathode ,Electronic, Optical and Magnetic Materials ,law.invention ,Anode ,Electrophoretic deposition ,Field electron emission ,Triode ,law ,Electrode ,Optoelectronics ,Electrical and Electronic Engineering ,business - Abstract
An electrophoretic process was developed to selectively assemble carbon nanotubes (CNTs) onto the triode structure and a CNT-based planar-gate-type electron source panel of planar gate stripe was successfully fabricated with the special electrophoretic process. In this process, the CNTs were migrated on cathode electrode in the CNT suspension by an applied voltage between the gate electrode and cathode electrode. The applied voltage was also used to keep the CNTs off adsorbing on the gate electrode. The experiment results show that the CNTs are selectively defined onto cathode electrode and each cathode electrode has the same packing density. In addition, field emission characteristics of the planar-gate-type electron source panel were studied. The anode current densities could be modified from 0 to 216 μA/cm 2 by increasing the gate voltages from 0 to 150 V with anode bias of 1400 V for anode–cathode spacing was 500 μm.
- Published
- 2008
50. Improved field emission characteristic of carbon nanotubes by an Ag micro-particle intermediation layer
- Author
-
Haifeng Zhao, Wenhui Lu, Hong Jiang, Zhiming Li, Hang Song, Guoqing Miao, and Yixin Jin
- Subjects
Materials science ,business.industry ,General Engineering ,Nanotechnology ,Substrate (electronics) ,Carbon nanotube ,law.invention ,Indium tin oxide ,Field electron emission ,Nanoelectronics ,law ,Electric field ,Optoelectronics ,business ,Current density ,Layer (electronics) - Abstract
An efficient way to improve field emission characteristic of carbon nanotubes (CNTs) through an Ag micro-particle intermediation layer is presented. In this way, the intermediation layer is deposited on an indium tin oxide glass substrate by electrochemical method and then the CNTs are covered onto surface of the intermediation layer by electrophoretic method as CNT field emitters. The field emission characteristic of the CNT field emitters with the intermediation layer is significantly improved compared to the one without the intermediation layer, including decreased turn-on electric field from 4.2 to 3.1V/@mm and increased emission current density from 0.224 to 0.912mA/cm^2 at an applied electric field of 6V/@mm. The improved field emission characteristic may be attributed to gibbous surface of the CNT field emitters. This efficient way is much simple, low cost, and suitable for production of large scale CNTs-based field emission cold cathode.
- Published
- 2008
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