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The prominent imagery of Menshen (门神door gods) within traditional Chinese culture has led to the development of a variety of cultural symbols, including military door gods, civil door gods, praying door gods, and other related ones, such as stone lions and Shigandang (stone tablets). This article studies the impact of the belief in Door Gods and their worship on Chinese psychology and behaviour on both a conscious and unconscious level. At the conscious level, from its first articulation to its development into a cultural image and related myths and legends, the belief in Door Gods can be said to have gone through four stages: a primitive worship of reproduction in ancient times, animal worship during the Zhou Dynasty, the worship of anthropomorphic gods during the Han Dynasty and the worship of hero gods worship during the Tang Dynasty. This process corresponds to the four specific symbols of ‘peach branch’, ‘tiger/chicken’, ‘Shēn Shū(神荼)’ and ‘Yù Lǜ(郁垒)’ (‘鬼’: the two spirits guarding the entrance of the house), and ‘hero’. On an unconscious level, the psychological symbolism of the belief in Door Gods belief is interpreted through the Door Gods sacrifice and the Fu(复)” hexagram. Closing the door is related to Kun (坤, the receptive, earth), while opening the door is related to Qian (乾, the creative, heaven). Together, Kun and Qian were held to be in a state of continual transition, one changing into the other, which reflects Chinese philosophy’s emphasis on movement. Traditionally, Chinese people held more than 10 kinds of door-related sacrificial activities every year. Although some of these activities have gradually fallen out of use, the traditional custom of pasting door couplets and images of Door Gods to doorways has been preserved. By repeating the ritual every year, the Chinese gain the strength to protect themselves and their family members. Clinical studies of sandplay therapy have found that the image of Door Gods constitutes a ‘patron saint’ on an unconscious level. Door gods guard the boundary between consciousness and unconsciousness (the inner and outer worlds), thereby protecting the spiritual strength of those who supplicate them. This suggests that using their images in a therapeutic context could help individuals to maintain boundaries and protect themselves. The emergence of the Door Gods image can transform the guardian energy hidden at the border between unconsciousness and consciousness, help the clients keep the boundary and protect themselves.

In order to improve the intelligence of the analysis of the health effects of social capital and psychological capital, this paper combines the data simulation model for data processing and makes a detailed analysis of the cloud model-related theories from the aspects of concept, digital characteristics, normal cloud, cloud generator, and so on. Moreover, this paper uses the cloud model to make a fuzzy data classification method, including the identification of knowledge features, the construction of fuzzy cloud model, the calculation of data attribution to the constructed cloud model, and the classification of knowledge and data. In addition, this paper verifies the health effect model of social capital and psychological capital based on the data simulation model proposed in this paper through experiments. The research results show that the intelligent model proposed in this paper has a good effect in the intelligent analysis of the health effects of social capital and psychological capital.

Objective: To investigate the role of ferroptosis in Antineutrophil cytoplasmic antibody (ANCA)-associated vasculitis (AAV) with renal injury. Methods: GSE104954 and GSE108112 were retrieved from the GEO database and concatenated into one dataset. Expression of ferroptosis-related genes (FRGs) was extracted for differential analysis. The ferroptosis signature genes were identified by LASSO regression and SVM-RFE, and their differential expression levels and diagnostic efficacy were verified by independent data sets. The ceRNA (miRNA-TF-mRNA) regulatory network and clinical diagnostic model were constructed respectively. By using consensus clustering, ferroptosis subtypes were identified. ssGSEA and GSVA were employed to assess immune response and pathway activation. Pan-cancer genes were found in TCGA and GTEx. Differential expression of CD44 in was validated by qPCR and immunohistochemistry from HPA database. Results:Twenty-four FRGs were differentially expressed in patients with AAV kidney injury. Furthermore, five ferroptosis signature genes were identified by two machine learning algorithms. Not only were differentially expressed in independent datasets, the clinical diagnostic model constructed by these genes provided reference for clinical decision-making, but also the ceRNA network revealed their complex regulatory mechanisms. Unsupervised clustering analysis discovered two ferroptosis subtypes with distinct gene expression, immunological microenvironment, and biological functioning pathways. Notably, CD44 was found to be closely associated with many immune cells, most immune responses, and HLA genes, as well as prognosis, immune cell infiltration, TMB, and MSI in patients with a variety of tumors, suggesting it may be a potential intervention target for human diseases including AAV renal injury and tumors. Conclusions:Ferroptosis in AAV with renal injury is significantly correlated with the immunological microenvironment. For AAV with renal injury and tumors, CD44 could be a useful intervention target.

This study used the Generalized Anxiety Disorder (GAD-7) and 9 Patients Health Questionnaire (PHQ-9) to investigate the anxiety and depression of 1366 primary school students in Shenzhen after the ...

Horizontal well drills are difficult to operate and require high borehole cleaning operations. The impact of cuttings accumulation on friction and torque cannot be ignored. In the process of horizontal well drilling, it is very easy to form a cuttings bed in the highly inclined eccentric annulus. The formation of the cuttings bed will not only increase the friction torque but also have a greater impact on the annulus flow. Based on the application of gas drilling in horizontal wells, this paper uses laboratory experiments to study the influence of cuttings with different particle sizes and lithology on the friction coefficient between the drill string and the borehole wall of the horizontal well section. In addition, in view of the difficulty in the migration of cuttings in gas drilling horizontal wells and the formation of cuttings deposits, this paper carries out an experimental study on the effect of the cuttings bed on drill pipe coating height and cuttings bed thickness on friction coefficient. Experimental results show that the friction coefficient of gas drilling horizontal wells is about 0.44–0.58. When the coating height of the cuttings on the drill pipe exceeds a certain value (26 mm), the friction coefficient tends to be stable, but its value is as high as about 0.55. When the thickness of the accumulation of cuttings exceeds a certain value (10 mm), the subsidence hindering effect between the particles in the cuttings bed is weakened, so the friction coefficient of the casing section and the open hole section tends to be stable, about 0.53.

Hyaluronan (HA) cross-linking is a conformational state of HA, a covalent complex between HA and heavy chains (HCs) from inter-α-trypsin inhibitor (I-α-I) mediated by tumor necrosis factor-induced protein 6 (TSG6). Cross-linked HA has been identified as a protective factor in physiological and inflammatory conditions. However, the state of HA cross-linking in tumor microenvironment has not been fully elucidated. As a major constituent of the extracellular matrix (ECM), HA is mainly synthesized by cancer-associated fibroblasts (CAFs). Our study aimed to clarify the role of HA cross-linking in breast cancer malignancy. Compared to normal mammary gland tissues, cross-linked HA levels were significantly decreased in breast cancer and associated with tumor malignancy. When NFbs were activated into CAFs, the levels of cross-linked HA and TSG6 were both suppressed. Through upregulating TSG6, CAFs restored the high level of cross-linked HA and significantly inhibited breast cancer malignancy, whereas NFbs promoted the malignancy when the cross-linked HA level was reduced. Furthermore, the inhibitory role of HA cross-linking in tumor malignancy was directly verified using the synthesized HA-HC complex. Collectively, our study found that the deficiency of cross-linked HA induced breast cancer malignancy in a CAF-dependent manner, suggesting that recovering HA cross-linking may be a potential therapeutic strategy.

Although luminal breast cancer cells are typically highly cohesive epithelial cells and have low invasive ability, many eventually develop metastasis. Until now, the underlying mechanisms remain obscure. In this work, we showed that the level of hyaluronic acid synthase 2 (HAS2) was positively correlated with the malignant phenotype of breast cancer cells. Notably, the increased expression of HAS2 promoted the invasive and migratory abilities of luminal breast cancer cells in vitro, followed by a reduced expression of E-cadherin, β-catenin, and ZO-1, and an elevated expression of N-cadherin and vimentin. Furthermore, overexpression of HAS2 promoted while knockdown of HAS2 impeded invadopodia formation, which subsequently increased or decreased the activation of cortactin, Tks5, and metalloproteinases (MMPs). Activation of these invadopodia-related proteins was prevented by inhibition of HAS2 or disruption of HA, which in turn attenuated the increased motility and invasiveness. Further, in vivo study showed that, HAS2 increased tumor growth and the rate of lung metastasis via driving transition to an invasive cell phenotype in SCID mice that were orthotopically transplanted with luminal breast cancer cells. Collectively, our results showed that HAS2 promoted cell invasion by inducing transition to an invasive phenotype and by enhancing invadopodia formation in luminal breast cancer cells, which may provide new mechanistic insights into its role in tumor metastasis.

In this study, 349 Han and 217 Tibetan college students were investigated via the Templer Death Anxiety Scale in order to assess the potential class and influencing factors of death anxiety among them. In addition, Mplus software was used to analyse the latent categories of their death anxiety, and an R3STEP approach was adopted to perform a multinomial logistic regression of its influencing factors. Whilst the results of the former indicated that there are two latent classes, respectively, defined as ‘high death anxiety type’ (Han 65.20%; Tibetan 30.30%) and ‘low death anxiety type’ (Han 34.80%; Tibetan 69.70%), the latter demonstrated that compared with the ‘low death anxiety type’, the occurrence ratio of the ‘high death anxiety type’ was 47.00 and 34.04 percentage points higher with each increase in age. Furthermore, the stress and anxiety of Han and Tibetan college students were found to constitute factors that affect death anxiety. More specifically, the death anxiety of Tibetan college students was determined to be deeply influenced by a belief in the afterlife.

Hyaluronan (HA) is dynamically remodeled in tumor microenvironment (TME) and is reported to be closely related to tumor lymphatic metastasis by inducing lymphangiogenesis. Macrophages are known to be involved in neo-lymphatic vessels formation. However, few studies have investigated the role of HA-mediated TME remodeling on macrophages-dependent lymphangiogenesis. We previously showed that HA could drive macrophages to acquire the M2 phenotype. In this study, we attempt to study the crosstalk between HA in TME and macrophages dependent lymphangiogenesis. First, we found that the abundant assembly of HA in breast cancer tissue was accompanied by increased infiltration of macrophages featured by expressing lymphatic endothelial markers. Then, to further identify the remodeling of HA in regulating macrophage phenotype, we used HA fragments which are usually enriched in TME for this purpose. Our results showed that the reconstructed HA could induce bone marrow-derived macrophages (BMDMs) to express markers of lymphatic endothelium and form tube-like structures, suggesting a novel function of HA from TME on macrophages-dependent lymphangiogenesis. Finally, we found that inhibition of the HA-TLR4 pathway could reduce the ability of BMDMs to exhibit lymphatic endothelial phenotype. Our results provide new insight into tumor microenvironment remodeling and macrophages in breast cancer lymphangiogenesis.

AISI 316L steel is often used in materials applied toward nuclear power but are subjected to pitting corrosion in a marine environment. In this study, (Cr, W, Al, Ti, Si)N multilayer coatings were deposited using multi-arc ion plating on the surface of non-nitrided and nitrided AISI 316L steel. The microstructure and corrosion resistance of four different systems were investigated, namely, (i) untreated AISI 316L steel, (ii) plasma nitrided (PN), (iii) coated on an untreated matrix (coating) only, and (iv) coated on nitrided (hybrid) specimens. The phase structures, morphologies, and compositions of the different specimens were characterized using X-ray diffraction, transmission electron microscope, Atomic Force Microscope, scanning electron microscope, X-ray photoelectron spectroscopy, and energy dispersive x-ray spectroscopy. The results show that a thin CrWAlTiSiN multilayer coating, approximately 2.3 μm in thickness, is deposited on the surface of an ~12 μm nitrided layer. Potentio-dynamic polarization and electrochemical impedance spectroscopy were used to evaluate the assessment of the electrochemical behavior in the natural seawater of China's Yellow Sea. The hybrid specimens exhibited excellent corrosion resistance compared to both the nitrided and coated specimens.

Most luminal breast carcinomas (BrCas) bearing PIK3CA mutations initially respond to phosphoinositide-3-kinase (PI3K)-α inhibitors, but many eventually become resistant. The underlying mechanisms of this resistance remain obscure. In this work, we showed that a CD44high state due to aberrant isoform splicing was acquired from adaptive resistance to a PI3Kα inhibitor (BLY719) in luminal BrCas. Notably, the expression of CD44 was positively correlated with estrogen receptor (ER) activity in PIK3CA-mutant breast cancers, and ER-dependent transcription upon PI3Kα pathway inhibition was in turn mediated by CD44. Furthermore, the interaction of CD44 with the ligand hyaluronan (HA) initiated the Src-ERK signaling cascade, which subsequently maintained AKT and mTOR activity in the presence of a PI3Kα inhibitor. Activation of this pathway was prevented by disruption of the CD44/HA interaction, which in turn restored sensitivity to BLY719. Our results revealed that an ER-CD44-HA signaling circuit that mediates robust compensatory activation of the Src-ERK signaling cascade may contribute to the development of acquired resistance to PI3Kα inhibitors. This study provides new insight into the mechanism of adaptive resistance to PI3Kα inhibition therapy.

Local joint inflammation plays an important role in the pathogenesis of temporomandibular joint (TMJ) osteoarthrosis (TMJOA). Yohimbine, an alpha-2 adrenergic receptor antagonist, possesses anti-inflammatory properties; however, the ability of Yohimbine to protect against TMJOA-associated chondrocyte inflammation remains unclear. We conducted in vitro and in vivo analyses to investigate whether Yohimbine could ameliorate TMJOA-induced chondrocyte inflammation and to elucidate the mechanisms involved. Chondrocytes of TMJOA mice were stimulated with interleukin (IL)-1β or noradrenaline (NE), and the resulting production of inflammation-related factors was evaluated in the presence or absence of Yohimbine. Furthermore, two TMJOA mouse models were treated with Yohimbine and the therapeutic effect was quantified. NE (10-6 M) triggered inflammatory cytokine secretion by TMJ chondrocytes, and Yohimbine suppressed IL-1β- or NE-induced IL-6 upregulation in TMJ chondrocytes with the nuclear factor (NF)-κB pathway inhibition. Yohimbine also ameliorated cartilage destruction in the TMJOA models. Interestingly, αmpT, a tyrosine hydroxylase inhibitor, reversed the effects of Yohimbine by activating the NF-κB pathway. Collectively, these findings show that Yohimbine ameliorated TMJ chondrocyte inflammation and the suppression of NF-κB pathway contributes to this effect.

Interleukin (IL)-1β is a key promotor in the pathogenesis of temporomandibular joint osteoarthritis. Differentiation of stem cells to cartilage is a crucial repair mechanism of articular cartilage damage, and IL-1β has been reported to impede the differentiation by upregulating the secretion of IL-6, an important inflammatory factor. Long non-coding RNAs (lncRNAs) regulate a number of physiological and pathological processes, but whether lncRNA AK094629 contributes to the IL-1β mediated induction of inflammation remains unclear. Therefore, the aim of the present study was to investigate the effect of AK094629 on IL-1β-induced IL-6 expression in synovial-derived mesenchymal stem cells (SMSCs) of the temporomandibular joints. The results of the present study demonstrated that the expression of AK094629 in the synovial tissue of patients with osteoarthritis was positively correlated with IL-1β. In addition, IL-1β upregulated the expression of AK094629 in the SMSCs in vitro, and AK094629 knockdown inhibited the IL-1β mediated upregulation of IL-6. The present study also demonstrated that AK094629 knockdown downregulated the expression of the mitogen-activated protein kinase kinase kinase 4 (MAP3K4), which is upregulated by IL-1β, whereas knockdown of MAP3K4 did not affect the expression of AK094629, but reversed the upregulation of IL-6 in SMSCs. In conclusion, AK094629 knockdown attenuated the expression of IL-1β-regulated IL-6 in the SMSCs of the temporomandibular joint by inhibiting MAP3K4. Therefore, AK094629 may be a potential novel therapeutic target for the treatment of temporomandibular joint osteoarthritis.

Synovium-derived mesenchymal stem cells (SMSCs) can migrate to the site of destroyed condylar cartilage and differentiate into chondrocytes to repair temporomandibular joint (TMJ) damage. Interleukin (IL)-1β-induced IL-6 secretion has been shown to inhibit the chondrogenic potential of SMSCs. The histone deacetylase inhibitor suberoylanilide hydroxamic acid (SAHA) has recently been shown to be closely related to the inflammation induced by IL-1β. However, the relationship between SAHA and IL-6 secretion induced by IL-1β in SMSCs remains unclear. In this study, we evaluated the relationships between IL-1β and IL-6 in synovial specimens from patients with TMD and in model rats with osteoarthritis (OA). We found that IL-1β and IL-6 were positively correlated and that IL-6 expression in SMSCs increased with IL-1β stimulation in vitro. Moreover, microtubule affinity-regulating kinase 4 (MARK4) was significantly upregulated in IL-1β-stimulated SMSCs and in the synovium of rats with OA. MARK4 knockdown inhibited IL-6 secretion and nuclear factor (NF)-κB pathway activation in IL-1β-stimulated SMSCs. SAHA attenuated IL-6 secretion in IL-1β-induced SMSCs through NF-κB pathway inhibition, and MARK4 was also downregulated in SAHA-treated SMSCs. However, inhibition of the NF-κB pathway did not suppress MARK4 expression. Thus, these results showed that SAHA attenuated IL-6 secretion in IL-1β-induced SMSCs through inhibition of the MARK4/NF-κB pathway.

Previous studies have shown that CD44 containing variant exon v6 (CD44v6) is highly expressed in many cancers and is related to tumor metastasis. However, the detailed mechanism of the regulatory pattern of CD44v6 in breast cancer remains unclear. Here, we found that CD44v6 was significantly upregulated in invasive breast cancer cell lines compared with low-invasive breast cancer cell lines. Cell migration and invasion could be suppressed by CD44v6 downregulation. MiRWalk and RNAhybrid software revealed miR-193b-5p as a miRNA targeting CD44v6 by binding to the exon v6 region. We found that the overexpression of miR-193b-5p inhibited the migration and invasion of Hs-578t and BT-549 cells, which could be rescued by restoring the expression of CD44v6. Next, we determined the potential of miR-193b-5p as an in vitro biomarker for breast cancer. Serum samples were obtained from 58 breast cancer patients, 36 patients with benign disease and 58 age-matched cancer-free controls. The results showed that the expression of miR-193b-5p in the serum was significantly lower in breast cancer patients than in controls and could distinguish cancer from cancer-free samples. The area under the receiver operating characteristic curve (ROC) for miR-193b-5p was 0.762(95% confidence interval: 0.674-0.851), which was higher than that of carcinoembryonic antigen (CEA) and cancer antigen 15-3 (CA15-3). Combining miR-193b-5p with CEA or CA15-3 could improve the diagnostic efficiency compared with the CEA and CA15-3 combination. Taken together, our results suggest that miR-193b-5p could function as a tumor-suppressive miRNA by targeting CD44v6 in breast cancer and that serum miR-193b-5p may serve as a biomarker for breast cancer diagnosis.

Objective: To understand the status quo of PICC Catheter-associated Skin Impairment (CASI) in some hospitals at all levels in Jingzhou city, and to explore the risk factors for PICC Catheter-associated Skin Impairment. Methods: From June 2019 to July 2020, a self-made questionnaire for CASI status was used to facilitate the extraction of CASI patients in PICC in some hospitals at all levels in Jingzhou city. Local skin lesions were collected and classified during PICC maintenance, and the damage types, occurrence frequency and related influencing factors of CASI were counted to identify independent risk factors. Results: In this study, there were 173 cases of CASI in PICC’s patients including local infection 56.1% (97/173), exudation at the puncture site 16.8% (29/173), irritant dermatitis 21.4% (37/173), allergic dermatitis 4.6% (8/173), and tension injury 1.2% (2/173). After univariate analysis, there were statistically significant differences between CASI occurrence type and duration of catheter wear, age, underlying disease, skin moisture and hyperhidrosis, PICC maintenance as required, hospital grade maintenance and intermittent chemotherapy (P < 0.05). Then, disordered multiple Logistic regression analysis was performed, and exudation at the puncture site was used as the reference group. The results showed that patients with PICC were more likely to have CASI if they were aged, long catheter time, had diabetes, had moist and sweaty skin, and were in the intermittent stage of chemotherapy. Conclusion: The incidence of CASI is high in patients aged 70 - 79 years, and local infection is the most common. PICC’s patients with diabetes are at high risk of CASI. PICC’s patients with excessive sweating are more likely to have CASI. The intermittent stage of chemotherapy is the excessive CASI; time with tube affects CASI occurrence.

The healthcare staff who supported Wuhan's rescue work were the first batch of cross-regional supporters during the outbreak of the COVID-19 pandemic. To determine the cultural factors that affected their mental health and resilience, as well as the processes that organizations and individuals underwent to transform cultural resources into benefits. This study collected narrative materials in the form of national and individual statements and used Social Ecology of Resilience theory to analyse them. It identified and analysed the cultural factors of resilience in macrosystems, mesosystems, and microsystems according to four themes, namely: cognition, emotion, will, and behaviour. Altogether, it was found that the national voice adopted an optimistic narrative tone reminiscent of that used during the context of war and that the personal or public voice approved of it. The study revealed that the party and government's use of moral narration derived from its heritage culture and its belief in its continuity in new China's culture served as a mechanism of cultural resilience and mobilisation. The above is the experience of the operation of living culture (LC) and cultural heritage (CH) mentioned in Culture Based Development (CBD). In light of this, it was concluded that the predominant form of cultural input during China's national public health crisis has been storytelling with narrative resilience.

Difficulties in collecting, processing, and identifying massive data have slowed research on cutting-edge science and technology hotspots. Promoting these technologies will not be successful without an effective data-driven method to identify cutting-edge technologies. This paper proposes a data-driven model for identifying global cutting-edge science technologies based on SpaCy. In this model, we collected data released by 17 well-known American technology media websites from July 2019 to July 2020 using web crawling with Python. We combine graph-based neural network learning with active learning as the research method in this paper. Next, we introduced a ten-fold cross-check to train the model through machine learning with repeated experiments. The experimental results show that this model performed very well in entity recognition tasks with an F value of 98.11%. The model provides an information source for cutting-edge technology identification. It can promote innovations in cutting-edge technologies through its effective identification and tracking and explore more efficient scientific and technological research work modes.

Background: Resistance to endocrine therapy is a major challenge for estrogen receptor-positive (ER+) breast cancer patients, but the underlying mechanisms remain unclear. Methods: Loss of hyaluronan synthase 2 (Has2) in adaptive resistant cells to tamoxifen and fulvestrant was observed by immunblotting assay. CRISPR/Cas9 technology was used to knock out Has2 in MCF7 cells to verify the effect of Has2 on the expression of ER and Ezrin and Akt and MAPK/ERK signaling routes. We utilized an Ezrin small-interfering RNA and Ezrin inhibitor to inhibit Ezrin expression for evaluating Has2 and ERα expression and the Akt/MAPK signaling cascade upon tamoxifen or fulvestrant treatment.Results: In this work, we showed that a Has2-loss state was acquired from adaptive resistance to tamoxifen and fulvestrant in luminal BrCas. Notably, the adapted loss of Has2 induced acquired resistance to antiestrogens in estrogen receptor (ER)-positive breast cancer cells through up-regulating the expression of Ezrin. Furthermore, we found that the loss of Has2 promoted while the consequent increase of Ezrin inhibited ERα expression/activity through the Akt and MAPK/ERK signaling routes, indicating an opposite effect on ERα expression during the development of antiestrogens-resistance. Inhibition of Ezrin reversed Has2 and ERα expression and the Akt/MAPK signaling cascade upon tamoxifen or fulvestrant, suggesting a Has2-Ezrin-ER negative-feedback loop in governing cellular sensitivity to tamoxifen or fulvestrant in luminal-like breast cancer cells. Finally, Knockdown or inhibition of Ezrin restored sensitivity to antiestrogens, implying that Ezrin could be a potential therapeutic target to tackle endocrine resistance. Conclusions: Taken together, our findings provide a direct relationship between ERα and Has2 implicated in resistance to endocrine therapy and a new insight into how ERα-signaling is regulated upon antiestrogens treatment, suggesting a novel therapeutic target for ER-positive breast cancer.

Yiqing He,1,2 Tao Li3 1Department of Psychology, School of Public Health, Southern Medical University, Guangzhou, People’s Republic of China; 2Institute of Analytical Psychology, City University of Macau, Macau, People’s Republic of China; 3Department of Radiology, Shenzhen Maternity & Child Healthcare Hospital, Shenzhen, People’s Republic of ChinaCorrespondence: Tao LiDepartment of Radiology, Shenzhen Maternity & Child Healthcare Hospital, No. 2004 Hongli Road, Futian District, Shenzhen, 518000, People’s Republic of ChinaEmail 404728242@qq.comBackground: College is a key period for students’ learning and development, as they begin independently to face life’s adversities. The essence of China’s zhongyong culture is to provide a practical way of thinking, a basic principle of the Chinese people. However, empirical research on psychopathology is lacking. The present study investigated Chinese college students to explore and explain the psychological mechanism of depressive symptoms via the zhongyong practical thinking mode.Methods: The study examined the relationship between zhongyong practical thinking and depressive symptoms, coping style, and ruminations of 501 Chinese college students. The statistical software SPSS was used to establish an intermediary model between moderate zhongyong practical thinking and depressive symptoms.Results: Rumination partially mediated the relationship between zhongyong practical thinking and depressive symptoms, but coping style had no mediating effect. Therefore, reducing the negative rumination behaviours of college students may be more conducive to alleviating their depressive symptoms. The chain mediating effect of coping style and rumination was significant. The college students with high levels of zhongyong thinking adopted positive coping styles to cope with various pressures and situations, reducing negative coping and rumination and thereby reducing their depressive symptoms.Discussion: The results of the study could help universities to provide courses and activities that promote students’ mental health from the perspective of zhongyong psychological interventions. The results provide a model for further studies of the influence of zhongyong practical thinking on depressive symptoms amongst college students.Keywords: zhongyong practical thinking, depression symptoms, rumination, coping style, college student mental health

Finding a way to move the economy away from rapid growth and toward high-quality development is essential for China’s economic success. Financial resilience and steady economic growth provide possible paths for high-quality economic development. This paper uses the panel data of 31 provinces and cities in mainland China from 2009 to 2020 to establish a comprehensive evaluation indicator system using the entropy weight method so as to measure the level of high-quality economic development and financial resilience of each province and city. On this basis, combined with the System-GMM estimation method of the dynamic panel system and the intermediary effect model, the impact of financial resilience and stable growth on high-quality economic development is demonstrated. The results show that financial resilience is essential for fostering high-quality economic development, and that steady growth plays a beneficial intermediary position in the connection between financial resilience and high-quality economic development. Furthermore, the impact of financial resilience and steady growth on high-quality economic development has obvious regional, developmental stage, and industrial structure heterogeneity characteristics. Therefore, enhancing financial resilience and boosting steady growth can serve the economy and boost high-quality economic development.

Collective invasion into adjacent tissue is a hallmark of luminal breast cancer, and ~20% of these cases eventually undergo metastasis. How less aggressive luminal-like breast cancer transitions to invasive cancer remains unclear. Our study revealed that CD44hi cancer cells are the leading subpopulation in collectively invading luminal cancer cells and efficiently promote the collective invasion of CD44lo/follower cells. The CD44hi/leader subpopulation showed a specific gene signature of various hybrid epithelial/mesenchymal genes and key functional coregulators of collective invasion, which was distinct from that of CD44lo/follower cells. However, the CD44hi/leader cells, which showed a partial epithelial-mesenchymal transition (EMT) phenotype, readily switched to the CD44lo phenotype along with collective migration and vice versa; this phenomenon was spontaneous and sensitive to the tumor microenvironment. The CD44lo-to-CD44hi conversion was accompanied by a shift in CD44s to CD44v but not a conversion of non-cancer stem cells to cancer stem cells (CSCs). Therefore, the CD44hi leader cells, as currently identified, are not a stable subpopulation in breast tumors. This plasticity and ability to generate CD44hi carcinoma cells with enhanced migratory and invasive behavior might be responsible for the transition from in situ to invasive behavior of luminal-type breast cancer.

Background Hyaluronidases (HAases), enzymes that degrade hyaluronan, have been widely investigated in cancer biology. However, whether HAases serve as tumor promoters or suppressors has been controversial in different cancers, and the exact role of HAases in colorectal cancer (CRC) has not been elucidated. Methods The expression levels of HYAL1, HYAL2, and HYAL3 in cancer and corresponding normal tissues from CRC patients were examined via immunohistochemistry. Then the correlation between HAases levels and pathological characteristics of CRC patients was analyzed. To verify the clinical data, HYAL1 and HYAL2 were downregulated or overexpressed in colon cancer cells LOVO and HCT116 to observe their influences on cell invasion and migration. For the mechanism study, we investigated the effects of HYAL1 and HYAL2 on the expression of matrix metalloproteases (MMPs)/tissue inhibitor of metalloproteases (TIMPs) and distribution of F-actin. Results All the three HAases were abnormally elevated in cancer tissues. Interestingly, HYAL1 and HYAL2, but not HYAL3, were negatively correlated with lymphatic metastasis and TNM stage. When HYAL1 and HYAL2 were knocked down, the invasion and migration abilities of colon cancer cells were accelerated, whereas overexpression of HYAL1 and HYAL2 had the opposite effects. In addition, colon cancer cells with HYAL1 and HYAL2 downregulation showed increased levels of MMP2 and MMP9, decreased levels of TIMP1 and TIMP2, and more intense F-actin stress fibers. Conclusions Our study suggests that HYAL1 and HYAL2 suppress CRC metastasis through regulating MMPs/TIMPs balance and rearranging F-actin distribution, further inhibiting invasion and migration of cancer cells.

The cell surface glycoprotein CD44 displays different active statuses; however, it remains unknown whether the activation process of CD44 is critical for tumor development and progression. The aim of the present study was to investigate whether breast cancer (BCa) cells with different activation states of CD44 show similar or distinct functional characteristics and to further examine the mechanisms regulating CD44 activities. A feature for the ‘activated’ state of CD44 is that it can bind to its principal ligand hyaluronan (HA). The binding of CD44 with HA is usually influenced by CD44 alternative splicing, resulting in multiple CD44 isoforms that determine CD44 activities. Flow cytometry was used to sort BCa cell subsets based on CD44-HA binding abilities (HA−/low vs. HAhigh). Subsequently, cell proliferation and colony formation assays were performed in vitro, and CD44 expression patterns were analyzed via western blotting. The results demonstrated that the CD44 variant isoform 10 (CD44v10) was highly expressed in a HA−/low binding subset of BCa cells, which exhibited a significantly higher proliferation capacity compared with the HAhigh binding subpopulation. Knockdown of CD44v10 isoform in HA−/low binding subpopulation induced an increase in HA binding ability and markedly inhibited proliferation. Furthermore, the mechanistic analysis identified that CD44v10 facilitated cell proliferation via activation of ERK/p38 MAPK and AKT/mTOR signaling. Moreover, the knockdown of CD44v10 expression downregulated the phosphorylation of ERK, AKT and mTOR, while no alteration was observed in p38 phosphorylation. Collectively, the present study identified a subset of fast-growing BCa cells characterized by CD44v10 expression, which may serve as a specific therapeutic target for BCa.

As the foundation of education, early childhood education has become a strategic focus of new curriculum reform in Shenzhen, China. In this study, 350 preschool teachers in Shenzhen were selected as research participants. Data were collected by means of three questionnaires: (1) Preschool Teachers’ Teaching Concept Scale, (2) Preschool Teachers’ Teaching Behavior Scale, and (3) Preschool Teachers’ Teaching Efficacy Scale , There is a significant positive correlation between teaching concept, teaching behavior and teaching efficacy of preschool teachers. Teaching concept and teaching behavior can significantly positively predict teaching efficacy. Therefore, reform of them are key factors in preschool teaching.