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Abstract During follicular development, changes in the composition of the follicular fluid are synchronized with the development of oocytes. Our aim was to screen the key factors affecting oocyte maturation and optimize the in vitro culture protocol by understanding the changes of proteins and metabolites in follicular fluid. Follicles are divided into three groups according to their diameter (small follicle fluid (SFF): 10 mm

Abstract Stimulating bone formation potentially suggests therapeutics for orthopedic diseases including osteoporosis and osteoarthritis. Osteoblasts are key to bone remodeling because they act as the only bone-forming cells. miR-877-5p has a chondrocyte-improving function in osteoarthritis, but its effect on osteoblast differentiation is unknown. Here, miR-877-5p-mediated osteoblast differentiation was studied. Real-time reverse transcriptase-polymerase chain reaction was performed to measure miR-877-5p expression during the osteogenic differentiation of MC3T3-E1 cells. Osteoblast markers, including alkaline phosphatase (ALP), collagen type I a1 chain, and osteopontin, were measured and detected by alizarin red staining and ALP staining. Potential targets of miR-877-5p were predicted from three different algorithms: starBase ( http://starbase.sysu.edu.cn/ ), PITA ( http://genie.weizmann.ac.il/pubs/mir07/mir07_data.html ), and miRanda ( http://www.microrna.org/microrna/home.do ). It was further verified by dual luciferase reporter gene assay. The experimental results found that miR-877-5p was upregulated during the osteogenic differentiation of MC3T3-E1 cells. Overexpression of miR-877-5p promoted osteogenic differentiation, which was characterized by increased cell mineralization, ALP activity, and osteogenesis-related gene expression. Knockdown of miR-877-5p produced the opposite result. Dual luciferase reporter gene assay showed that miR-877-5p directly targeted eukaryotic translation initiation factor 4γ2 (EIF4G2). Overexpression of EIF4G2 inhibited osteogenic differentiation and reversed the promoting effect of overexpression of miR-135-5p on osteogenic differentiation. These results indicate that miR-877-5p might have a therapeutic application related to its promotion of bone formation through targeting EIF4G2.

Abstract Objective Osteogenesis is the key process of bone homeostasis differentiation. Numerous studies have manifested that circular RNA (circRNA) is a critical regulator of osteogenesis. The research was to explore circRNA-mediated mechanisms in osteogenesis. Methods Bone marrow mesenchymal stem cells (BMSCs) were cultured and induced to osteogenic differentiation (OD). Then, oe-circ-FKBP5, oe-NC, si-circ-FKBP5, si-NC, miR-205-5p mimic, mimic NC, miR-205-5p inhibitor, inhibitor NC, sh-RUNX2, or sh-NC were transfected into BMSCs. Alkaline phosphatase (ALP) activity was detected by ALP staining, cell mineralization was detected by alizarin red staining, cell proliferation was detected by CCK-8, and cell apoptosis was detected by flow cytometry. Then, the expression of circ-FKBP5, miR-205-5p, RUNX2 and osteogenic marker genes was detected by RT-qPCR, and the expression of RUNX2 protein was detected by Western blot. Finally, the targeting relationship between miR-205-5p and circ-FKBP5 or RUNX2 was verified by bioinformation website analysis and dual luciferase reporter gene detection. Results Circ-FK501 binding protein 51 (FKBP5) was distinctly elevated during OD of BMSCs. Elevated circ-FKBP5 boosted the proliferation and OD, as well as expression of osteogenic marker genes while reduced apoptosis of BMSCs. Down-regulation of circ-FKBP5 inhibited BMSCs proliferation, OD and osteogenic marker gene expression, and promoted apoptosis of BMSCs. Subsequently, circ-FKBP5 combined with miR-205-5p and constrained miR-205-5p expression. Silenced miR-205-5p boosted proliferation, OD, and expression of osteogenic marker genes and suppressed apoptosis of BMSCs. However, up-regulation of miR-205-5p inhibited BMSC proliferation, OD and osteogenic marker gene expression, and promoted apoptosis. Additionally, miR-205-5p targeted Runt-associated transcription factor 2 (RUNX2). Repression of RUNX2 turned around the effect of circ-FKBP5 overexpression on BMSCs. Conclusion In brief, circ-FKBP5 boosted BMSC proliferation and OD by mediating the miR-205-5p/RUNX2 axis.

To investigate molecular regulation involved in lactation during pregnancy, this study focused on the transcriptomic profiles of mammary tissue from lactating and non-lactating Mongolian mares at the second month of gestation. A total of 4197 differentially expressed genes were identified by comparing mammary tissues from pregnant mares at two different states, including 1974 differentially expressed genes such as the milk protein-related genes a-s1-casein (CSN1S1), k-casein (CSN3), lactalbumin (LALBA), and lactoferrin (LTF), which were highly expressed in the lactating mares group, and overall, these differentially expressed genes were mainly associated with biological processes such as endoplasmic reticulum protein processing, the Toll-like receptor signaling pathway, steroid biosynthesis, cytokine–cytokine receptor interactions, and amino sugar and nucleotide glycolysis. These findings serve as a foundation for investigating the molecular underpinnings of lactation in pregnant equids.

To discover novel antioxidants and anticoagulants for the prevention and treatment of cardiovascular diseases remains ongoing concern. Sagittaria trifolia is a commonly consumed aquatic vegetable with highly nutritious value and wide planting area in China. Polysaccharides are the major bioactive constituents, but little information is available on anticoagulant potential. In this work, a polysaccharide from S. trifolia (PST) was modified by sulfation to produce four sulfated derivatives (SPST-1 ∼ 4), and their antioxidant and anticoagulant potentials were evaluated. Various means of analysis confirmed the success of sulfation on PST. The unmodified PST possessed notable antioxidant and pro-coagulant activities. To some extent, sulfated modification reduced the antioxidant capacity of PST with the increase of degree of substitution (DS), but for the derivative SPST-4 with moderate DS and smallest molecular weight (MW) exerted better antioxidant activity, in particular of scavenging nitrogen-containing radicals. Interestingly, sulfation reversed the innate activity of PST from pro-coagulation to anticoagulation, which highly depended on DS. The derivative SPST-3 with highest DS and moderate MW elicited promising anticoagulant potential, particularly at concentration of 5.00 mg/mL, which was superior to that of heparin (positive control) in the aspect of prolonging thrombin time and prothrombin time. Herein, the present contribution would give evidence that PST and sulfated derivatives SPST-3 and SPST-4 own potentials to be developed as hemostatic, anticoagulant, and antioxidant candidates, respectively.

During equine early gestation, trophectoderm forms chorion tissue, which is composed of two parts that one is covering allantoin, called allantochorion (AC) and another is covering yolk sac, which here we call vitelline-chorion (VC). Given that little is known about the equine trophoblast-derived chorion differentiation at an early stage, we first compared the transcriptome of AC and VC of day 30 equine conceptus based on RNA-sequencing. As a result, we found that compared to VC, there are 484 DEGs, including 305 up- and 179 down-regulated genes in AC. GO and KEGG analysis indicated that up-regulated genes in AC are mainly cell proliferation and cell adhesion-related genes, participating in allantois expansion and allantochorionic-placenta formation; dominant genes in VC are extracellular exosome and other cell adhesion-related genes implicated in direct and indirect conceptus-maternal communication. Additionally, as for the progenitor chorion tissue of equine chorionic gonadotropin secreting endometrium cup—the chorionic girdle (CG), which locates at the junction of the dilating AC and regressing VC, we revealed its unique gene expression pattern and the gene regulation during its further differentiation in vitro. Collectively, this study sheds light on the molecular events regarding the trophoblast differentiation and function at an early stage of the equine preimplantation conceptus.

Abstract Background Exercise intervention can significantly improve physical function and bone strength; however, the effect of exercise on fall-related fractures in older adults remains controversial. This study aimed to assess the effectiveness of exercise intervention on fall-related fractures in older adults by conducting a meta-analysis of randomized controlled trials (RCTs). Methods PubMed, EMBASE, and Cochrane databases were systematically searched for RCTs through November 24, 2019 to investigate the effectiveness of exercise intervention on fall-related fractures in older adults. Pooled relative risk (RR) with 95% confidence interval (CI) was calculated using the random-effects model. Sensitivity, subgroup, and publication bias analyses were also conducted. Results A total of 7704 older adults and 428 fall-related fracture events from 20 RCTs were selected for the final meta-analysis. The follow-up duration across included trials ranged from 6.0 months to 7.0 years. The pooled RR suggested that exercise intervention was associated with a reduced fall-related fracture risk in older adults (RR: 0.74; 95% CI: 0.59–0.92; P = 0.007; I 2 = 12.6%). The pooled conclusion was robust and not affected by any individual trial. Subgroup analysis revealed that the significant effect of exercise intervention on fall-related fractures was mainly detected when the study reported results from both male and female subjects, when it did not report the baseline body mass index, when individuals received both home- and center-based interventions, when the follow-up duration was > 1.0 year, and when it was a high-quality study. Conclusions Regular exercise intervention could prevent fall-related fractures in older adults. Further large-scale RCTs should be conducted to assess the effectiveness of different exercise programs on fall-related fractures at various sites.

Mungbean (Vigna radiata L.), one of the most widely grown edible legumes in Asia, plays important roles in the improvement of agricultural cultivation systems as well as human diets. As an understudied crop, however, most mungbean varieties are characterized by unstable yields, poor disease resistance, and unsuitability for mechanical harvesting, thereby leading to a low production income. We, therefore, developed Jilv 20, a new mungbean cultivar with a dull seed coat, by crossing Bao 942-34 with Weilv 9002-341. After 11 years of selection and evaluation, Jilv 20 has shown wide adaptability, early maturity, high yield, halo blight resistance, and suitability for mechanical harvesting. The growth period of Jilv 20 is 79.5 and 66.7 days in the spring and summer sowing seasons, respectively, and the average plant height is approximately 56.4 cm. In this study, average yields were 1737.9, 1532.3, and 2045.1 kg/hm2 in northern spring-sowing, northern summer-sowing, and southern sites, respectively; these values were respectively 27.83%, 28.48%, and 6.96% higher than those of control cultivar Zhonglv 5, which has been popular in past decades because of its wide adaptability. The average protein and starch contents of Jilv 20 seeds were 25.0% and 49.56%, respectively. Further application and extension of Jilv 20 in China should contribute to mungbean production, breeding, and industrial development.

The Ferredoxin-NADP reductase (FNR) gene plays a significant role in NADPH production, carbon assimilation, antioxidation, and cross-talking between chloroplasts and mitochondria in plants. This study aims to know the functional response of the soybean FNR gene (GmFNR) during a soybean mosaic virus (SMV) infection. For this purpose, we developed the bean pod mottle virus (BPMV)-based gene construct (BPMV-GmFNR) and used it to silence the GmFNR gene in resistant and susceptible lines. The results showed that GmFNR expression decreased to 50% in the susceptible line, compared to 40% in the resistant line. The silencing of GmFNR reduces the photosynthetic capacity and CAT activity of both lines compared to their respective controls. In addition, the H2O2 content increased significantly in the susceptible line, whereas the resistant line did not exhibit any change. Further, an SMV infection in the silencing plants of the susceptible line resulted in serious morphological changes and increased the SMV NIa-protease transcript accumulation compared to its control plants. However, the same impact was not observed in the resistant line. The yeast two-hybrid system, BIFC assay, and quantitative real-time polymerase chain reaction (qRT-PCR) analyses revealed that the GmFNR was interacting with EF1A and coincided with the increased SMV accumulation. The results obtained in this study improve the understanding of the soybean FNR gene response during SMV infection and provide a novel insight into the SMV resistance mechanism.

The incidence rate of breast cancer is high, and endocrine therapy for breast cancer frequently causes bone loss. We conducted a systematic review and meta-analysis to review the effect of TCM on bone loss associated with endocrine therapy in patients with breast cancer. Medline (Ovid), EMBASE, Cochrane Library, Web of Science, China National Knowledge Infrastructure (CNKI), VIP full-text Database, Wanfang Database, Chinese Biomedical Literature data (CBM), relevant Chinese and foreign periodicals, conference papers, degree papers, and supplemented by literature tracing were searched. Data from randomized controlled trials (RCTs) on the effects of TCM on bone loss associated with endocrine therapy in patients with breast cancer were collected according to the inclusion and exclusion criteria from the establishment of each database to May 2022. Twenty-two clinical controlled studies were eventually selected. The results of the meta-analysis showed effectiveness (RR (Risk Ratio): 1.22, 95% CI (confidence interval): 1.10-1.36, I² = 0%), improvement of lumbar bone mineral density (BMD) (WMD (weighted mean difference): 0.07, 95% CI: 0.06-0.08, I² = 23%), improvement of BMD T value (WMD: 0.37, 95% CI: 0.28-0.46, I² = 66%), and improvement of BMD in the femoral neck (WMD: 0.07, 95% CI: 0.05-0.09, I² = 33%). The funnel chart suggested that a publication bias was observed in the literature, which may be explained by the heterogeneity in the study data and the limited number of included examples. Sensitivity analysis was conducted to confirm that the differences among the studies were acceptable. No study reported adverse effects. Our results indicated that TCM is helpful for bone loss associated with endocrine therapy in patients with breast cancer based on the effectiveness, lumbar BMD, BMD T value, and BMD of the femoral neck. The use of TCM is thus safe. [ABSTRACT FROM AUTHOR]

The leaves of soybean cultivar ZheA8901 show various symptoms (necrosis, mosaic, and symptomless) when infected with different strains of soybean mosaic virus (SMV). Based on a proteomic analysis performed with tandem mass tags (TMT), 736 proteins were differentially expressed from soybean samples that showed asymptomatic, mosaic, and necrosis symptoms induced by SMV strains SC3, SC7, and SC15, respectively. Among these, GmGSTU13 and ascorbate peroxidase (APX) were only upregulated in mosaic and symptomless leaves, respectively. The protein level of GmGSTU13 determined by western blot analysis was consistent with TMT analysis, and quantitative reverse transcriptase PCR analysis showed that GmGSTU13 mRNA levels in mosaic plants were 5.26- and 3.75-fold higher than those in necrotic and symptomless plants, respectively. Additionally, the expression of the viral coat protein (CP) gene was increased, and serious mosaic symptoms were observed in GmGSTU13-overexpressing plants inoculated with all three SMV strains. These results showed that GmGSTU13 is associated with the development of SMV-induced mosaic symptoms in soybean and that APX is upregulated in symptomless leaves at both the transcriptional and protein levels. In APX gene-silenced soybean plants, the relative expression of the viral CP gene was 1.50, 7.59, and 1.30 times higher than in positive control plants inoculated with the three SMV strains, suggesting that the upregulation of APX may be associated with lack of symptoms in soybean infected with SMV. This work provides a useful dataset for identifying key proteins responsible for symptom development in soybean infected with different SMV strains. [ABSTRACT FROM AUTHOR]