7 results on '"Ying-qun Zhu"'
Search Results
2. Current Status of the Treatment of COPD in China: A Multicenter Prospective Observational Study
- Author
-
Li-Bing Ma, Xin Li, Mei-ling Zhou, Shan Cai, Ping Chen, Jia-Xi Duan, Yuqin Zeng, Yan Chen, Qi-mi Liu, Ying-Qun Zhu, Ming Chen, and Yiyang Zhao
- Subjects
medicine.medical_specialty ,COPD ,biology ,business.industry ,medicine.medical_treatment ,General Medicine ,Lama ,medicine.disease ,biology.organism_classification ,Group B ,03 medical and health sciences ,FEV1/FVC ratio ,0302 clinical medicine ,030228 respiratory system ,Internal medicine ,Oxygen therapy ,medicine ,Observational study ,030212 general & internal medicine ,Positive pressure ventilation ,business ,Lung function - Abstract
Background There is a large gap in the treatments for patients with COPD according to the Global Initiative for COPD (GOLD) recommendations. Determining the situation of therapies in the real world is necessary. This study aimed to characterize the real-world practical therapies of COPD and prognosis of patients after treatment for 1 year. Methods This study was a multicenter prospective observational study performed using a database set up by the Second Xiangya Hospital of Center South University. Detailed usage information for pharmacotherapies and nonpharmacotherapies for patients was collected, as well as the consistency of recommendations and patient adherence. Moreover, the effect of therapies after 1 year was calculated by comparing lung function and symptoms. Results Ultimately, 4,796 patients with COPD from 12 hospitals in China were eligible. LAMA (39.1%), LAMA + LABA/ICS (39.0%) and LABA/ICS (14.4%) were the top three inhalants. We found that 42.7% of Group A patients, 61.6% of Group B patients and 30% of Group C patients were following inappropriate therapy, especially overuse of ICS. Only 3.9% (95% CI 2.4, 5.4) of patients used oxygen therapy, and 1.8% (95% CI 1.5, 2.3) used noninvasive positive pressure ventilation at home. Among these patients, 33.2% had poor adherence. A total of 452 patients completed 1 year of follow-up. After 1 year of treatment, the lung function of FEV1/FVC decreased (P=0.001) and the mMRC score increased (P 0.05). Conclusion This study highlights a significant discrepancy between recommendations for managing patients with COPD in GOLD report, and in real-world clinical practice in China. Over-prescription of ICS and under-prescription of nonpharmacologic therapy were common. The adherence to treatment of patients was poor, and the real-life treatment effectiveness was unsatisfactory. More attention should be paid to the implementation of recommendations and standardized administration of therapies.
- Published
- 2020
- Full Text
- View/download PDF
3. Characteristics of Patients with Chronic Obstructive Pulmonary Disease Exposed to Different Environmental Risk Factors: A Large Cross-Sectional Study
- Author
-
Li-Bing Ma, Xin Li, Jia-Xi Duan, Ying-Qun Zhu, Qi-mi Liu, Mei-ling Zhou, Wei Cheng, Ming Chen, Shan Cai, Ping Chen, Yuqin Zeng, and Yan Chen
- Subjects
Male ,medicine.medical_specialty ,Vital capacity ,Cross-sectional study ,International Journal of Chronic Obstructive Pulmonary Disease ,tobacco ,chronic obstructive pulmonary disease ,Pulmonary Disease, Chronic Obstructive ,03 medical and health sciences ,FEV1/FVC ratio ,0302 clinical medicine ,Risk Factors ,Forced Expiratory Volume ,Smoke ,Internal medicine ,medicine ,Humans ,030212 general & internal medicine ,Respiratory system ,Original Research ,Aged ,COPD ,business.industry ,Smoking ,Confounding ,occupational exposure ,General Medicine ,medicine.disease ,Cross-Sectional Studies ,030228 respiratory system ,biomass smoke ,Cohort ,Female ,Observational study ,business - Abstract
Jia-xi Duan,1,2 Wei Cheng,1,2 Yu-qin Zeng,1,2 Yan Chen,1,2 Shan Cai,1,2 Xin Li,3 Ying-qun Zhu,4 Ming Chen,5 Mei-ling Zhou,6 Li-bing Ma,7 Qi-mi Liu,8 Ping Chen1,2 1Department of Pulmonary and Critical Care Medicine, The Second Xiangya Hospital, Central South University, Changsha, Hunan 410011, People’s Republic of China; 2Research Unit of Respiratory Disease, Central South University, Changsha, Hunan 410011, People’s Republic of China; 3Division 4 of Occupational Diseases, Hunan Prevention and Treatment Institute for Occupational Diseases, Changsha, Hunan 410000, People’s Republic of China; 4Department of Respiratory Medicine, The Third Hospital of Changsha, Changsha, Hunan 410011, People’s Republic of China; 5Department of Respiratory Medicine, The No.1 Traditional Chinese Medicine Hospital in Changde, Changde, Hunan 415000, People’s Republic of China; 6Department of Respiratory Medicine, The First People’s Hospital of Huaihua, Huaihua, Hunan 418000, People’s Republic of China; 7Department of Respiratory Medicine, Affiliated Hospital of Guilin Medical University, Guilin, Guangxi 541000, People’s Republic of China; 8Department of Respiratory and Critical Care Medicine, The Second People’s Hospital of Guilin, Guilin, Guangxi 541000, People’s Republic of ChinaCorrespondence: Ping ChenDepartment of Pulmonary and Critical Care Medicine, The Second Xiangya Hospital, Central South University, 139 Renmin Middle Road, Changsha, Hunan 410011, People’s Republic of ChinaTel +86 731 8529 5248Email pingchen0731@csu.edu.cnPurpose: Tobacco smoking, biomass smoke, and occupational exposure are the main risk factors for chronic obstructive pulmonary disease (COPD). The present study analyzes data on exposure to these factors in a cohort of patients with COPD and assesses their differences in demographic and clinical characteristics.Patients and Methods: The cross-sectional observational study was conducted from November 2016 to December 2019. Inclusion criteria were patients aged over 40 years old with post-bronchodilator forced expiratory volume in 1 second (FEV1)/forced vital capacity (FVC) < 0.7. At baseline, demographic features and exposure history were recorded. Moreover, respiratory symptoms were assessed by the COPD Assessment Test (CAT) and modified Medical Research Council scale (mMRC). A generalized linear mixed model was used to adjust for potential confounders.Results: A total of 5183 patients with COPD were included in the final analysis. The results demonstrate that exposure to tobacco combined with other risk factors resulted in significantly higher CAT scores (16.0 ± 6.7 vs 15.3 ± 6.3, P = 0.003) and more severe dyspnea (patients with mMRC ≥ 2, 71.5% vs 61.6%, P < 0.001) than exposure to tobacco alone. In addition, COPD patients with biomass smoke exposure alone had higher CAT scores than patients with only tobacco or occupational exposure (17.5 ± 6.3 vs 15.3 ± 6.3, and 15.2 ± 6.3, respectively, P < 0.05 for each comparison) and were more likely to be female and older. In addition, COPD patients who suffered from occupational exposure developed more severe dyspnea than those exposed to tobacco alone (70.8% vs 61.6%, P < 0.05), as did those exposed to biomass smoke alone (74.2% vs 61.6%, P < 0.05). This difference remained strong even after adjustment for potential confounders.Conclusion: There are significant demographic and clinical differences among COPD patients with tobacco smoking, biomass smoke, and occupational exposures.Keywords: chronic obstructive pulmonary disease, tobacco, biomass smoke, occupational exposure
- Published
- 2020
4. KLF2 regulates neutrophil migration by modulating CXCR1 and CXCR2 in asthma
- Author
-
Dan-dan Shi, Yu-zhu Li, Zi Wang, Li-ming Zhu, Ai-guo Dai, Jin Huang, Yan-feng Deng, Jing Liu, Xue-chun Lei, Yu-bin Ji, Ying-qun Zhu, Dan Zeng, and Fang-fang Dai
- Subjects
0301 basic medicine ,Male ,Neutrophils ,Guinea Pigs ,Kruppel-Like Transcription Factors ,Biology ,Receptors, Interleukin-8B ,Receptors, Interleukin-8A ,03 medical and health sciences ,Chemokine receptor ,0302 clinical medicine ,Downregulation and upregulation ,Cell Movement ,Animals ,Humans ,CXC chemokine receptors ,Receptor ,Molecular Biology ,Transcription factor ,Aged ,Gene knockdown ,Middle Aged ,Asthma ,respiratory tract diseases ,030104 developmental biology ,030228 respiratory system ,KLF2 ,Immunology ,T cell migration ,Molecular Medicine ,Female - Abstract
Neutrophils are key inflammatory cells in the immunopathogenesis of asthma. Neutrophil migration can be initiated through activation of the CXCR1 and CXCR2 receptors by CXC chemokines, such as IL-8. Although transcription factor KLF2 has been found to maintain T cell migration patterns through repression of several chemokine receptors, whether KLF2 can regulate neutrophil migration via modulation of CXCR1 and CXCR2 is unknown. Here, we aimed to explore the functions of KLF2, CXCR1 and CXCR2 in neutrophil migration in asthma and to establish a regulatory role of KLF2 for CXCR1/2. We demonstrate that with asthma aggravation, the percentages and migration rates of peripheral blood neutrophils gradually increased in asthmatic patients and the guinea pig asthma model. Correspondingly, both the KLF2 mRNA and protein levels in neutrophils were gradually reduced. While CXCR1 and CXCR2 expression was negatively correlated with KLF2. In vitro knockdown of KLF2 dramatically increased the migration of HL-60-drived neutrophil-like cells, which was accompanied by an increase in the CXCR1 and CXCR2 mRNA and protein expression levels. Taken together, our results indicate that decreased KLF2 aggravates asthma progression by promoting neutrophil migration, which is associated with the transcriptional upregulation of CXCR1 and CXCR2. The KLF2 and/or CXCR1/2 expression levels may represent an indicator of asthma severity.
- Published
- 2019
5. Evaluation of Meropenem Regimens Suppressing Emergence of Resistance in Acinetobacter baumannii with Human Simulated Exposure in an In Vitro Intravenous-Infusion Hollow-Fiber Infection Model
- Author
-
Yang Yang, Xian-Jia Zhang, Ying-Qun Zhu, Lin Wang, Wei Liu, Wei-Tao Gong, Bin Xu, and Xin Li
- Subjects
Acinetobacter baumannii ,Drug ,media_common.quotation_subject ,Microbial Sensitivity Tests ,Drug resistance ,Biology ,medicine.disease_cause ,Meropenem ,Microbiology ,Drug Resistance, Multiple, Bacterial ,medicine ,Humans ,Potency ,Experimental Therapeutics ,Pharmacology (medical) ,media_common ,Pharmacology ,Pseudomonas aeruginosa ,bacterial infections and mycoses ,Bactericidal effect ,biology.organism_classification ,In vitro ,Anti-Bacterial Agents ,Infectious Diseases ,Carbapenems ,Thienamycins ,Acinetobacter Infections ,medicine.drug - Abstract
The emergence of resistance to carbapenems in Pseudomonas aeruginosa can be suppressed by optimizing the administration of meropenem. However, whether the same is true for Acinetobacter baumannii is not fully understood. We assessed the bactericidal activity of meropenem and its potency to suppress the emergence of resistance in A. baumannii with human simulated exposure in an in vitro intravenous-infusion hollow-fiber infection model (HFIM). Two clinical strains of carbapenem-susceptible multidrug-resistant A. baumannii (CS-MDRAB), CSRA24 and CSRA91, were used, and their MICs and mutant prevention concentrations (MPCs) were determined. Six meropenem dosage regimens (0.5, 1.0, or 2.0 g given every 8 h [q8h] with a 0.5-h or 3-h infusion for seven consecutive days) were simulated and then evaluated in the HFIM. Both the total population and resistant subpopulations of the two strains were quantified. Drug concentrations were measured by high-performance liquid chromatography. All dosage regimens, except for the lowest dosage (0.5 g for both the 0.5-h and 3-h infusions), showed 3-log CFU/ml bacterial killing. Dosage regimens of 2.0 g with 0.5-h and 3-h infusions exhibited an obvious bactericidal effect and suppressed resistance. Selective amplification of subpopulations with reduced susceptibility to meropenem was suppressed with a percentage of the dosage interval in which meropenem concentrations exceeded the MPC ( T >MPC) of ≥20% or with a ratio of T >MPC to the percentage of the dosage interval in which drug concentrations are within the mutant selection window of ≥0.25. Our in vitro data support the use of a high dosage of meropenem (2.0 g q8h) for the treatment of severe infection caused by CS-MDRAB.
- Published
- 2014
- Full Text
- View/download PDF
6. PRMT6 mediates CSE induced inflammation and apoptosis
- Author
-
Naixing Kang, Xue He, Ping Chen, Yan Chen, Huihui Zeng, and Ying-Qun Zhu
- Subjects
Protein-Arginine N-Methyltransferases ,Arginine ,Immunology ,Down-Regulation ,Inflammation ,Apoptosis ,Biology ,Methylation ,Umbilical vein ,Cell Line ,Histones ,Histone H3 ,Pulmonary Disease, Chronic Obstructive ,Annexin ,parasitic diseases ,Tobacco ,medicine ,Human Umbilical Vein Endothelial Cells ,Immunology and Allergy ,Humans ,Transgenes ,Pharmacology ,Smoking ,Nuclear Proteins ,Molecular biology ,Blot ,Reverse transcription polymerase chain reaction ,embryonic structures ,cardiovascular system ,medicine.symptom - Abstract
Cigarette smoke extract (CSE) induces apoptosis and inflammation, but the mechanism is unknown. Arginine methyltransferase (PRMT6) catalyzes the asymmetric di-methylation of histone H3 arginine 2 (H3R2me2a) to control global level transcription. We hypothesized that PRMT6 mediates CSE induced apoptosis and inflammation through H3R2me2a. The apoptosis after CSE treatment in human umbilical vein endothelial cells (HUVECs) was fully measured with real-time reverse transcription PCR, western blotting and Annexin-V staining. Meanwhile, the inflammation in HUVECs after CSE exposure was detected with real-time reverse transcription PCR, western blotting and ELISA. CSE treatment promoted apoptosis and inflammation in HUVECs, coinciding with the decreased protein abundance of PRMT6. Meanwhile, HUVECs transfected with PRMT6 expressing plasmid inhibited the CSE-induced apoptosis and inflammation. Also, the inhibition of PRMT6 promoted the apoptosis and inflammation in HUVECs induced by CSE. Notably, H3R2me2a was associated with the modulation of PRMT6 in CSE induced apoptosis and inflammation in HUVECs. In conclusion, PRMT6 mediates CSE induced apoptosis and inflammation through H3R2me2a in HUVECs.
- Published
- 2014
7. Dual Effects of Cigarette Smoke Extract on Proliferation of Endothelial Progenitor Cells and the Protective Effect of 5-aza-2′-deoxycytidine on EPCs against the Damage Caused by CSE
- Author
-
Zhi-Hui He, Yue Yang, Ying-Qun Zhu, Ji-Ru Ye, Da Liu, Sheng-Dong He, Yan Chen, and Ping Chen
- Subjects
Time Factors ,Article Subject ,Decitabine ,lcsh:Medicine ,General Biochemistry, Genetics and Molecular Biology ,Flow cytometry ,Andrology ,Neovascularization ,chemistry.chemical_compound ,parasitic diseases ,Medicine ,Animals ,Progenitor cell ,Cell Shape ,Cells, Cultured ,Cell Proliferation ,Endothelial Progenitor Cells ,General Immunology and Microbiology ,medicine.diagnostic_test ,business.industry ,lcsh:R ,Smoking ,General Medicine ,Flow Cytometry ,In vitro ,Mice, Inbred C57BL ,medicine.anatomical_structure ,chemistry ,Cytoprotection ,DNA methylation ,embryonic structures ,cardiovascular system ,Azacitidine ,Deoxycytidine ,Bone marrow ,medicine.symptom ,business ,Biomarkers ,medicine.drug ,circulatory and respiratory physiology ,Research Article - Abstract
Cigarette smoke is a major public health problem associated with multitude of diseases, including pulmonary and vascular diseases. Endothelial progenitor cells (EPCs) contribute to neovascularization and play an important role in the development of these diseases. The effect of CSE on EPCs is seldom studied. The aim of the current study is to observe the effect of CSE on biological behavior of EPCs and, further, to search for potential candidate agent in protection of proliferation of EPCs against the damage caused by CSE exposurein vitro.Methods. The proliferations of EPCs isolated from bone marrow of C57BL/6J mice were assessed by MTT after incubating the EPCs with a series of concentrations of CSE (1.0%, 2.5%, 5.0%, and 10.0%) for different times (3, 6, and 24 hours) as well as with 1.0% CSE in presence of 5-AZA-CdR for 24 hours.Results. The proliferations of EPCs were significantly enhanced after 3 hours of exposure to concentrations of 1.0% and 2.5% CSE but depressed when exposed to concentrations of 5.0% and 10.0% CSE. Furthermore, the 5-AZA-CdR in concentrations of 2.0 μmol/L and 5.0 μmol/L partly protected against the depression of proliferation of EPCs caused by CSE exposure.Conclusions. The CSE showed dual effects on proliferation of EPCs isolated from mice. The 5-AZA-CdR partly protected the proliferation of EPCs against the damage caused by CSE exposurein vitro, suggesting that DNA methylation may be involved in the dysfunction of EPCs induced by CSE.
- Published
- 2014
Catalog
Discovery Service for Jio Institute Digital Library
For full access to our library's resources, please sign in.