11 results on '"Ying-Jing Wang"'
Search Results
2. Delayed metformin treatment improves functional recovery following traumatic brain injury via central AMPK-dependent brain tissue repair
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Jie Guo, Mingsheng Zhang, Bao-Long Niu, Mei-Na Wu, Ying-Jing Wang, Xiang-Ying Jiao, Cai-Hong Yang, Yan Li, Jing Zhao, Yanying Fan, Min Chen, and Yu Li
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Male ,0301 basic medicine ,medicine.medical_specialty ,Traumatic brain injury ,Glial scar ,Mice ,03 medical and health sciences ,0302 clinical medicine ,Internal medicine ,Brain Injuries, Traumatic ,medicine ,Animals ,Hypoglycemic Agents ,Phosphorylation ,Gap-43 protein ,Protein kinase A ,Microglia ,biology ,business.industry ,General Neuroscience ,Adenylate Kinase ,Brain ,AMPK ,Recovery of Function ,medicine.disease ,Adenosine ,Metformin ,Disease Models, Animal ,Neuroprotective Agents ,030104 developmental biology ,medicine.anatomical_structure ,Endocrinology ,Motor Skills ,biology.protein ,business ,030217 neurology & neurosurgery ,medicine.drug - Abstract
Accumulating evidence suggests that chronic metformin posttreatment offers potent neuroreparative effects against acute brain injury. However, in previous studies, metformin was not initially administered beyond 24 h postinjury, and the effects of delayed metformin treatment in traumatic brain injury (TBI) and other types of acute brain injury and the related mechanisms are unclear. To test this, male C57BL/6 mice received once daily metformin treatment (20, 50 or 100 mg/kg/d, i.p.) at day 1–14, day 1–2, day 1–10, day 3–10, day 5–12 or day 5–28 after cryogenic TBI (cTBI). The results showed that 100 mg/kg/d metformin administered at day 1–14 postinjury significantly promoted motor functional recovery in the beam walking and gait tests and reduced the infarct volume. Metformin (100 mg/kg/d) administered at day 1–10 or day 3–10 but not day 1–2 or day 5–12 after cTBI significantly improved motor functional outcomes at day 7 and 14, and reduced the infarct volume at day 14. Interestingly, the therapeutic time window was further expanded when the duration of metformin treatment starting at day 5 postinjury was extended to 2 weeks. Furthermore, compared with cTBI, the administration of metformin at day 3–10 or day 5–28 after cTBI significantly elevated the expression of phosphorylated adenosine monophosphate-activated protein kinase (AMPK) and growth associated protein 43 (an axonal regeneration marker) and the number of vascular branch points and decreased the area of glial scar and the number of amoeboid microglia in the peri-infarct area at day 14 or 28 postinjury. The above beneficial effects of metformin were blocked by the intracerebroventricular injection of the AMPK inhibitor compound C (40 μg/mouse/d). Our data provide the first evidence that metformin has a wide therapeutic time window for at least 5 days after cTBI, during which it can improve functional recovery by promoting tissue repair and inhibiting glial scar formation and microglial activation in a central AMPK-dependent manner.
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- 2020
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3. Contrasting whole-rock and mineral compositions of ore-bearing (Tongchang) and ore-barren (Shilicun) granitic plutons in SW China: Implications for petrogenesis and ore genesis
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Xian-Wu Bi, Ying-Jing Wang, Ming-Liang Huang, Rui Ma, Xin-Song Wang, Leiluo Xu, Ruizhong Hu, Gong Liu, and Yongyong Tang
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010504 meteorology & atmospheric sciences ,Pluton ,Geochemistry ,Geology ,engineering.material ,010502 geochemistry & geophysics ,01 natural sciences ,Ore genesis ,Geochemistry and Petrology ,Magma ,engineering ,Mafic ,Amphibole ,Biotite ,0105 earth and related environmental sciences ,Zircon ,Petrogenesis - Abstract
A common perception is that oxidized magma is critical for the formation of a porphyry Cu ± Mo ± Au ore deposit. We have used an ore-bearing pluton (Tongchang) and an ore-barren pluton (Shilicun) in the western rim of the South China block to test this idea and to determine other important controls on ore genesis. Zircon U Pb ages indicate that the Tongchang and Shilicun plutons were emplaced at 36.3 ± 0.2 Ma and 35.2 ± 0.4 Ma, respectively, broadly coinciding with strike-slip faulting in the region and continental collision that occurred ~300 km to the west. These two plutons are all characterized by significant light REE enrichments and pronounced negative Nb Ta anomalies, similar to the bulk crust. Apatite separates from these two plutons all have elevated initial 87Sr/86Sr (Tongchang, 0.70690 to 0.70796; Shilicun, 0.70703 to 0.70726) and negative eNd(t) (Tongchang, −6.2 to −7.3; Shilicun, −4.5 to −5.8). The mean eHf(t) and δ18O of zircon from these plutons are −1.4 and 6.8‰ for Tongchang, and −0.3 and 6.5‰ for Shilicun. The Sr-Nd-Hf isotope compositions are all within the ranges of the Neoproterozoic mafic arc lower crust in the region. Whole-rock U-Nb-Ta systematics indicate that the Tongchang and Shilicun plutons originated from an amphibole-bearing source and a garnet-bearing source, respectively, implying a shallower and more H2O-rich mafic source for the former than the latter. Quartz trace element data indicate that the Tongchang pluton was emplaced at a shallower depth than the Shilicun pluton. Zircon Ce4+/Ce3+ and whole-rock V/Sc ratios, and apatite SO3 and MnO contents indicate that the parental magmas of both plutons are as oxidized as those of some porphyry Cu deposits in northern Chile, but the parental magma of the Tongchang pluton is less oxidized than that of the Shilicun pluton. Apatite Cl-F-OH systematics and higher abundant hydrous silicate minerals such as amphibole and biotite in the Tongchang pluton than the Shilicun pluton indicate that the parental magma of the Tongchang pluton has higher Cl and H2O content than that of the Shilicun pluton. Our results show that oxidized magma is important but this alone cannot make a porphyry Cu ± Mo ± Au ore deposit. High contents of H2O-Cl in magma and shallow depth of emplacement are also important.
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- 2019
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4. Molecular networking as a dereplication strategy for monitoring metabolites of natural product treated cancer cells
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Cheng-Chih Hsu, Hsin-Hsiang Chung, Yi-Ling Gao, Ko-Chien Chen, Ying-Jing Wang, and Tang-Long Shen
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Biological Products ,Natural product ,Leukemia ,Cordycepin ,biology ,Drug discovery ,Organic Chemistry ,Antineoplastic Agents ,Tandem mass spectrometry ,biology.organism_classification ,Analytical Chemistry ,chemistry.chemical_compound ,Metabolomics ,chemistry ,Biochemistry ,Cell culture ,Tandem Mass Spectrometry ,Cell Line, Tumor ,Cancer cell ,Cordyceps militaris ,Cordyceps ,Drug Discovery ,Metabolome ,Humans ,Spectroscopy - Abstract
Rationale Natural products have been great sources for drug discovery. However, the structures of natural products are diverse and difficult to elucidate. Cordyceps militaris is a parasitic fungus which usually grows on host insects. The metabolites of C. militaris have been reported to act as chemotherapeutic agents. In this study, we aimed for the structural elucidation of specialized metabolites derived from C. militaris, and the metabolic impact in leukemia cells. Methods We describe a liquid chromatography data-dependent mass spectrometric platform combining tandem mass analysis and molecular networking. Leukemia cells treated with C. militaris extract and control groups were visualized in terms of their metabolic profiles using Global Natural Product Social (GNPS) molecular networking. By this method, we were able to elucidate the structures of metabolites from medicinal fungus extracts and cancer cells and then to recognize their changes in a semi-quantitative manner. Results Using C. militaris and leukemia cells as examples, we found that approximately 100 new ion species were present in the treated leukemia cells, suggesting a highly altered metabolic profile. Specifically, based on the tandem mass spectral similarity, we proposed that cordycepin, a key fungus-derived therapeutic agent known for its antitumor activity, was transformed into its methylthio form in leukemia cells. Conclusions The platform described provides an ability to investigate complex molecular interactions of natural products in mammalian cells. By incorporating tandem mass spectrometry and molecular networking, we were able to reveal the chemical modification of crude bioactive compounds, for example potential bioactive compounds which might be modified from cordycepin. We envision that such a mass spectrometry (MS)-based workflow, combined with other metabolomics platforms, would enable much wider applicability to cell biology and be of great potential to pharmacological study as well as drug discovery.
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- 2019
5. Intraplate adakite-like rocks formed by differentiation of mantle-derived mafic magmas: A case study of Eocene intermediate-felsic porphyries in the Machangqing porphyry Cu-Au mining district, SE Tibetan plateau
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Ying-Jing Wang, Xin-Song Wang, Xian-Wu Bi, Yan Tao, and Ting-Guang Lan
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geography ,geography.geographical_feature_category ,Felsic ,Fractional crystallization (geology) ,010504 meteorology & atmospheric sciences ,Geochemistry ,Geology ,010502 geochemistry & geophysics ,01 natural sciences ,Diorite ,Volcanic rock ,Igneous rock ,Adakite ,Phenocryst ,Mafic ,0105 earth and related environmental sciences ,Earth-Surface Processes - Abstract
Intraplate intermediate-felsic adakite-like rocks are widely considered to be generated via partial melting of the mafic lower crust. However, the genesis of phenocrysts of mafic minerals from some adakite-like rocks suggests that they can originate from mantle-derived magmas through assimilation and fractional crystallization (AFC) processes. In this study, we investigated the petrogenesis of three phases of Eocene porphyries, namely quartz diorite porphyry (QDP-I and QDP-II), granodiorite porphyry (GDP), and granite porphyry (GP), exposed in the Machangqing area of the western Yangtze Craton in the southeastern Tibetan plateau. These porphyries were formed at 34.2–35.2 Ma in an intracontinental extension setting and present adakitic affinities of high Sr/Y (50–95) and La/Yb (50–113) ratios. The similar characteristics of these Machangqing porphyries in terms of whole-rock trace elements and Sr–Nd isotopes, as well as in the mineralogy of amphibole phenocrysts and clinopyroxene antecrysts, indicate that these intermediate-felsic porphyries were derived from the same source. The important observation for investigating the parental magma of these intermediate-felsic porphyries is that they have close temporal and spatial correlations with mantle-derived mafic volcanic rocks. These intermediate-felsic porphyries have slightly enriched Sr–Nd isotopic compositions [(87Sr/86Sr)i = 0.7068–0.7077, eNd(t) = −5.1 to −6.6], affected by limited crustal assimilation, as compared to those of contemporaneous mafic volcanic rocks. The high-Al amphibole phenocrysts and clinopyroxene antecrysts in QDP-I have similar rare earth element (REE) patterns with clinopyroxene phenocrysts in the mafic volcanic rocks, which suggest that these rocks originated from the same mantle-derived mafic magma. In addition, the major elements of these intermediate-felsic and mafic igneous rocks in the Machangqing area show fractional crystallization trends with an increasing magma silica component. Simulations of the major and trace elements of these igneous rocks show that the sequence of fractional crystallization of olivine, clinopyroxene, garnet, amphiboles, biotite, feldspar, and apatite with time promoted the evolution of the magma series. As revealed by the calculated physicochemical conditions during crystallization of the phenocrysts, the initial mafic magmas were derived from depths of greater than 64 km through the partial melting of the metasomatic lithosphere mantle induced by Eocene intraplate extension in the western Yangtze Craton. These initial mafic magmas underwent three stages of fractional crystallization at three levels of the thickened crust: the first at the base of the lower crust (49–55 km); followed by fractional crystallization at the middle crust (24–32 km); and finally at the upper crust (4–5 km). These fractional crystallization processes caused the initial mafic magmas to evolve through the intermediate (roughly represented by QDP-I) to the felsic compositions (GDP and GP). Whole-rock and mineral geochemistry evidence demonstrates that fractional crystallization of mantle-derived magmas, with a small amount of crustal assimilation, generated these Eocene intraplate intermediate-felsic adakitic porphyries in the Machangqing area.
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- 2020
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6. Polygalacturonic acid hydrogel with short-chain hyaluronate cross-linker to prevent postoperative adhesion
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Jia-Wei Chen, Hsiu-Hui Peng, Chung-Fan Kuo, Ming-Wei Lee, Ying-Jing Wang, and Tzi-Peng Yang
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Adipic acid ,Polymers and Plastics ,Bioengineering ,Postoperative adhesion ,Biomaterials ,chemistry.chemical_compound ,stomatognathic system ,chemistry ,Galacturonic acid ,Polymer chemistry ,Hyaluronic acid ,Materials Chemistry ,Hydrogel membrane ,Cross linker - Abstract
The purpose of this study was to develop a poly(galacturonic acid) (PGA)-based hydrogel using a short-chain hyaluronate (sHA) cross-linker for medical applications. PGA was grafted with adipic acid dihydrazide (ADH) to yield PGA–ADH, an amine-containing PGA derivative. This PGA–ADH formed a water-insoluble hydrogel by reacting with 1,1′-carbonyldiimidazole (CDI)–grafted sHA (sHA–CDI) in aqueous solution. The sHA–cross-linked PGA hydrogel has a water content of about 94%–97% and compressive modulus of 10.7–26.9 kPa. The in vitro data indicated that the sHA–cross-linked PGA hydrogel is degradable and noncytotoxic, thus suitable for biomedical applications. Animal implant studies showed that the sHA–cross-linked PGA hydrogel membrane exhibited antiadhesion potency, significantly higher than that found in untreated rats and has great potential for future clinical applications.
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- 2011
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7. Ophiocordyceps formosana improves hyperglycemia and depression-like behavior in an STZ-induced diabetic mouse model
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Ko-Chien Chen, Richard Chu, Ying-Jing Wang, Tzu-Wen Hong, Shih-Torng Ding, Lee-Yan Sheen, Satoru Takahashi, Tang-Long Shen, Sheng-Hong Tsai, Wen-Sung Lai, Chao-Wei Huang, Wei-Cheng Chen, Tai-Yuan Chuang, and Ju-Chun Pei
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0301 basic medicine ,Blood Glucose ,Male ,medicine.medical_specialty ,Adipose tissue ,Adipokine ,Ophiocordyceps formosana ,Streptozocin ,Diabetes Mellitus, Experimental ,03 medical and health sciences ,chemistry.chemical_compound ,Mice ,0302 clinical medicine ,Internal medicine ,Diabetes mellitus ,medicine ,Animals ,Glucose tolerance test ,Biological Products ,Insulin insensitivity ,medicine.diagnostic_test ,Cordycepin ,Adiponectin ,Behavior, Animal ,business.industry ,Depression ,Body Weight ,General Medicine ,Streptozotocin ,medicine.disease ,Mice, Inbred C57BL ,030104 developmental biology ,Endocrinology ,Complementary and alternative medicine ,chemistry ,Hyperglycemia ,Hypocreales ,business ,Rosiglitazone ,030217 neurology & neurosurgery ,medicine.drug ,Research Article - Abstract
Background A newly defined Cordyceps species, Ophiocordyceps formosana (O. formosana) has been implicated in multitudinous bioactivities, including lowering glucose and cholesterol levels and modulating the immune system. However, few literatures demonstrate sufficient evidence to support these proposed functions. Although the use of Cordyceps spp. has been previously addressed to improve insulin insensitivity and improve the detrimental symptoms of depression; its mechanistic nature remains unsettled. Herein, we reveal the effects of O. formosana in ameliorating hyperglycemia accompanied with depression. Methods Diabetes was induced in mice by employing streptozotocin(STZ), a chemical that is toxic to insulin-producing β cells of the pancreas. These streptozotocin (STZ)-induced diabetic mice showed combined symptoms of hyperglycemia and depressive behaviors. Twenty-four STZ-induced mice were randomly divided into 3 groups subjected to oral gavage with 100 μL solution of either PBS or 25 mg/mL Ophiocordyceps formosana extract (OFE) or 2 mg/mL rosiglitazone (Rosi, positive control group). Treatments were administered once per day for 28 days. An additional 6 mice without STZ induction were treated with PBS to serve as the control group. Insulin sensitivity was measured by a glucose tolerance test and levels of adiponectin in plasma and adipose tissue were also quantified. Behavioral tests were conducted and levels of monoamines in various brain regions relating to depression were evaluated. Results HPLC analysis uncovered three major constituents, adenosine, D-mannitol and cordycepin, within O. formosana similar to other prestigious medicinal Cordyceps spp.. STZ-induced diabetic mice demonstrated decreased body weight and subcutaneous adipose tissue, while these symptoms were recovered in mice receiving OFE treatment. Moreover, the OFE group displayed improved insulin sensitivity and elevated adiponectin within the plasma and adipose tissue. The anti-depressive effect of OFE was observed in various depression-related behavior tests. Concurrently, neurotransmitters, like 5-HT and dopamine in the frontal cortex, striatum and hippocampus were found to be up-regulated in OFE-treated mice. Conclusions Our findings illustrated, for the first time, the medicinal merits of O. formosana on Type I diabetes and hyperglycemia-induced depression. OFE were found to promote the expression of adiponectin, which is an adipokine involved in insulin sensitivity and hold anti-depressive effects. In addition, OFE administration also displayed altered levels of neurotransmitters in certain brain regions that may have contributed to its anti-depressive effect. Collectively, this current study provided insights to the potential therapeutic effects of O. formosana extracts in regards to hyperglycemia and its depressive complications.
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- 2015
8. Evaluation of an Epitypified Ophiocordyceps formosana (Cordyceps s.l.) for Its Pharmacological Potential
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Tang-Long Shen, Tzu Wen Hong, Yen Wen Wang, Pham Thi Kim Lien, Kenji Irie, Richard Chu, Shih-Torng Ding, S. S. Tzean, Tsai-Kun Li, Tzu-Ho Chou, Yu Ling Tai, Jui Ya Lai, Ying Jing Wang, and Sheng-Hong Tsai
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Antitumor activity ,Ophiocordyceps formosana ,Cordyceps ,Article Subject ,biology ,Traditional medicine ,lcsh:Other systems of medicine ,biology.organism_classification ,lcsh:RZ201-999 ,Complementary and alternative medicine ,Metabolite profiling ,Botany ,Complementary medicine ,Mycelium ,Research Article - Abstract
The substantial merit ofCordyceps s.l.spp. in terms of medicinal benefits is largely appreciated. Nevertheless, only few studies have characterized and examined the clinical complications of the use of health tonics containing these species. Here, we epitypifiedC. formosanaisolates that were collected and characterized asOphiocordyceps formosanabased on morphological characteristics, molecular phylogenetic analyses, and metabolite profiling. Thus, we renamed and transferredC. formosanato the new protologueOphiocordyceps formosana(Kobayasi & Shimizu) Wang, Tsai, Tzean & Shencomb. nov.Additionally, the pharmacological potential ofO. formosanawas evaluated based on the hot-water extract from its mycelium. The relative amounts of the known bioactive ingredients that are unique toCordyceps s.l.species inO. formosanawere found to be similar to the amounts inO. sinensisandC. militaris, indicating the potential applicability ofO. formosanafor pharmacological uses. Additionally, we found thatO. formosanaexhibited antioxidation activitiesin vitroandin vivothat were similar to those ofO. sinensisandC. militaris. Furthermore,O. formosanaalso displayed conspicuously effective antitumor activity compared with the testedCordyceps s.l.species. Intrinsically,O. formosanaexhibited less toxicity than the otherCordycepsspecies. Together, our data suggest that the metabolites ofO. formosanamay play active roles in complementary medicine.
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- 2015
9. Research on enterprise habitual domains evolving theory and its application
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Jin Chen, Ying-Jing Wang, and Lei Ma
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Computer science ,business.industry ,Artificial intelligence ,business - Abstract
For the sake of making the enterprise habitual theory play an important role in practice, it is not enough to research this theory only to the extent of the concept. The law of evolution of this theory should be found out and deeply studied. The hierarchy of enterprise habitual system has been analyzed to prove its self-organization. Based on the synergetics theory, the simplified model of enterprise organization habitual domains (EOHD) has been established in an ideal condition to discuss the solution of its stationary state and then the time-delay characteristics of EOHD evolution have been gained. The results of research have also been used to analyze the enterprise merge & acquisition (M&A) problem.
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- 2005
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10. Ophiocordyceps formosana improves hyperglycemia and depression-like behavior in an STZ-induced diabetic mouse model.
- Author
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Chao-Wei Huang, Tzu-Wen Hong, Ying-Jing Wang, Ko-Chien Chen, Ju-Chun Pei, Tai-Yuan Chuang, Wen-Sung Lai, Sheng-Hong Tsai, Chu, Richard, Wei-Cheng Chen, Lee-Yan Sheen, Satoru Takahashi, Shih-Torng Ding, and Tang-Long Shen
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MENTAL depression ,ANTIDEPRESSANTS ,AMINE analysis ,DOPAMINE analysis ,ADENOSINES ,ADIPOSE tissues ,AMINOGLYCOSIDES ,ANIMAL behavior ,ANIMAL experimentation ,BIOLOGICAL models ,BODY weight ,FUNGI ,GLUCOSE tolerance tests ,HIGH performance liquid chromatography ,HYPERGLYCEMIA ,IMMUNOHISTOCHEMISTRY ,INSULIN resistance ,TYPE 1 diabetes ,MICE ,PROBABILITY theory ,RESEARCH funding ,STATISTICAL sampling ,SEROTONIN ,STATISTICS ,T-test (Statistics) ,DATA analysis ,MANNITOL ,ROSIGLITAZONE ,DATA analysis software ,ADIPONECTIN ,DESCRIPTIVE statistics ,ONE-way analysis of variance - Abstract
Background: A newly defined Cordyceps species, Ophiocordyceps formosana (O. formosana) has been implicated in multitudinous bioactivities, including lowering glucose and cholesterol levels and modulating the immune system. However, few literatures demonstrate sufficient evidence to support these proposed functions. Although the use of Cordyceps spp. has been previously addressed to improve insulin insensitivity and improve the detrimental symptoms of depression; its mechanistic nature remains unsettled. Herein, we reveal the effects of O. formosana in ameliorating hyperglycemia accompanied with depression. Methods: Diabetes was induced in mice by employing streptozotocin(STZ), a chemical that is toxic to insulin-producing β cells of the pancreas. These streptozotocin (STZ)-induced diabetic mice showed combined symptoms of hyperglycemia and depressive behaviors. Twenty-four STZ-induced mice were randomly divided into 3 groups subjected to oral gavage with 100 µL solution of either PBS or 25 mg/mL Ophiocordyceps formosana extract (OFE) or 2 mg/mL rosiglitazone (Rosi, positive control group). Treatments were administered once per day for 28 days. An additional 6 mice without STZ induction were treated with PBS to serve as the control group. Insulin sensitivity was measured by a glucose tolerance test and levels of adiponectin in plasma and adipose tissue were also quantified. Behavioral tests were conducted and levels of monoamines in various brain regions relating to depression were evaluated. Results: HPLC analysis uncovered three major constituents, adenosine, D-mannitol and cordycepin, within O. formosana similar to other prestigious medicinal Cordyceps spp.. STZ-induced diabetic mice demonstrated decreased body weight and subcutaneous adipose tissue, while these symptoms were recovered in mice receiving OFE treatment. Moreover, the OFE group displayed improved insulin sensitivity and elevated adiponectin within the plasma and adipose tissue. The anti-depressive effect of OFE was observed in various depression-related behavior tests. Concurrently, neurotransmitters, like 5-HT and dopamine in the frontal cortex, striatum and hippocampus were found to be up-regulated in OFE-treated mice. Conclusions: Our findings illustrated, for the first time, the medicinal merits of O. formosana on Type I diabetes and hyperglycemia-induced depression. OFE were found to promote the expression of adiponectin, which is an adipokine involved in insulin sensitivity and hold anti-depressive effects. In addition, OFE administration also displayed altered levels of neurotransmitters in certain brain regions that may have contributed to its anti-depressive effect. Collectively, this current study provided insights to the potential therapeutic effects of O. formosana extracts in regards to hyperglycemia and its depressive complications. [ABSTRACT FROM AUTHOR]
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- 2016
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11. Research on enterprise habitual domains evolving theory and its application.
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Lei Ma, Jin Chen, and Ying-Jing Wang
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- 2004
- Full Text
- View/download PDF
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