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14 results on '"Ying-Feng Huang"'

1. Landscapes and mechanisms of CD8+ T cell exhaustion in gastrointestinal cancer

Author

Jia-Tong Ding, Kang-Ping Yang, Hao-Nan Zhou, Ying-Feng Huang, Hui Li, and Zhen Zong

Subjects
T cell exhaustion, gastric cancer, colorectal cancer, immune checkpoints, immunotherapy, Immunologic diseases. Allergy, RC581-607
Abstract

CD8+ T cells, a cytotoxic T lymphocyte, are a key component of the tumor immune system, but they enter a hyporeactive T cell state in long-term chronic inflammation, and how to rescue this depleted state is a key direction of research. Current studies on CD8+ T cell exhaustion have found that the mechanisms responsible for their heterogeneity and differential kinetics may be closely related to transcription factors and epigenetic regulation, which may serve as biomarkers and potential immunotherapeutic targets to guide treatment. Although the importance of T cell exhaustion in tumor immunotherapy cannot be overstated, studies have pointed out that gastric cancer tissues have a better anti-tumor T cell composition compared to other cancer tissues, which may indicate that gastrointestinal cancers have more promising prospects for the development of precision-targeted immunotherapy. Therefore, the present study will focus on the mechanisms involved in the development of CD8+ T cell exhaustion, and then review the landscapes and mechanisms of T cell exhaustion in gastrointestinal cancer as well as clinical applications, which will provide a clear vision for the development of future immunotherapies.

Published
2023
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2. TGF-β Pathways Stratify Colorectal Cancer into Two Subtypes with Distinct Cartilage Oligomeric Matrix Protein (COMP) Expression-Related Characteristics

Author

Jia-Tong Ding, Hao-Nan Zhou, Ying-Feng Huang, Jie Peng, Hao-Yu Huang, Hao Yi, Zhen Zong, and Zhi-Kun Ning

Subjects
colorectal cancer, COMP, TGF-β, Microbiology, QR1-502
Abstract

Background: Colorectal cancers (CRCs) continue to be the leading cause of cancer-related deaths worldwide. The exact landscape of the molecular features of TGF-β pathway-inducing CRCs remains uncharacterized. Methods: Unsupervised hierarchical clustering was performed to stratify samples into two clusters based on the differences in TGF-β pathways. Weighted gene co-expression network analysis was applied to identify the key gene modules mediating the different characteristics between two subtypes. An algorithm integrating the least absolute shrinkage and selection operator (LASSO), XGBoost, and random forest regression was performed to narrow down the candidate genes. Further bioinformatic analyses were performed focusing on COMP-related immune infiltration and functions. Results: The integrated machine learning algorithm identified COMP as the hub gene, which exhibited a significant predictive value for two subtypes with an area under the curve (AUC) value equaling 0.91. Further bioinformatic analysis revealed that COMP was significantly upregulated in various cancers, especially in advanced CRCs, and regulated the immune infiltration, especially M2 macrophages and cancer-associated fibroblasts in CRCs. Conclusions: Comprehensive immune analysis and experimental validation demonstrate that COMP is a reliable signature for subtype prediction. Our results could provide a new point for TGFβ-targeted anticancer drugs and contribute to guiding clinical decision making for CRC patients.

Published
2022
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4. Landscapes and mechanisms of CD8+ T cell exhaustion in gastrointestinal cancer.

Author

Jia-Tong Ding, Kang-Ping Yang, Hao-Nan Zhou, Ying-Feng Huang, Hui Li, and Zhen Zong

Subjects
T-cell exhaustion, GASTROINTESTINAL cancer, CYTOTOXIC T cells, T cells, STOMACH cancer
Abstract

CD8+ T cells, a cytotoxic T lymphocyte, are a key component of the tumor immune system, but they enter a hyporeactive T cell state in long-term chronic inflammation, and how to rescue this depleted state is a key direction of research. Current studies on CD8+ T cell exhaustion have found that the mechanisms responsible for their heterogeneity and differential kinetics may be closely related to transcription factors and epigenetic regulation, which may serve as biomarkers and potential immunotherapeutic targets to guide treatment. Although the importance of T cell exhaustion in tumor immunotherapy cannot be overstated, studies have pointed out that gastric cancer tissues have a better anti-tumor T cell composition compared to other cancer tissues, which may indicate that gastrointestinal cancers have more promising prospects for the development of precision-targeted immunotherapy. Therefore, the present study will focus on the mechanisms involved in the development of CD8+ T cell exhaustion, and then review the landscapes and mechanisms of T cell exhaustion in gastrointestinal cancer as well as clinical applications, which will provide a clear vision for the development of future immunotherapies. [ABSTRACT FROM AUTHOR]

Published
2023
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5. The TGF-β/Smad

Author

Zhong Lin, Yu, Jiang, Liu, Zhi Kun, Ning, Hua Kai, Tian, Xun, Wu, Ying Feng, Huang, Zi Chun, Wu, Zhen, Zong, and Tai Cheng, Zhou

Abstract

To identify Musashi2 as an effective biomarker regulated by the TGF-β/Smad

Published
2022

6. Microarray analysis of altered long non-coding RNA expression profile in liver cancer cells treated by ginsenoside Rh2

Author

Zhi-Bing Hu, Yi-Min Liu, Hong-Xia Xiao, Wei-Wen Chen, Ying-Zi Zhuang, Da-Bin Liu, and Ying-Feng Huang

Subjects
Carcinoma, Hepatocellular, Ginsenosides, Microarray, Pharmaceutical Science, Biology, Real-Time Polymerase Chain Reaction, 01 natural sciences, Analytical Chemistry, law.invention, chemistry.chemical_compound, Downregulation and upregulation, law, Drug Discovery, medicine, Humans, Polymerase chain reaction, Pharmacology, 010405 organic chemistry, Microarray analysis techniques, Liver Neoplasms, Organic Chemistry, Hep G2 Cells, General Medicine, Microarray Analysis, medicine.disease, Long non-coding RNA, 0104 chemical sciences, 010404 medicinal & biomolecular chemistry, Gene Ontology, Complementary and alternative medicine, chemistry, Ginsenoside, Hepatocellular carcinoma, Cancer research, Molecular Medicine, RNA, Long Noncoding, Liver cancer, Signal Transduction
Abstract

Microarray expression profiles of lncRNAs and mRNAs were investigated in HepG2 cells treated with 20 μg/ml ginsenoside Rh2 as well as in ginsenoside Rh2-untreated cells. Microarray analysis showed 618 upregulated lncRNAs and 161 downregulated lncRNAs in HepG2 cells treated with ginsenoside Rh2 compared with the control group. Moreover, three differentially expressed lncRNAs were validated by quantitative real-time polymerase chain reaction (qRT-PCR). This may be beneficial to patients as an anti-cancer treatment and potentially provide novel targets for HCC (hepatocellular carcinoma) therapy.

Published
2018

7. Up-regulation of the tumor promoter Glyoxalase-1 indicates poor prognosis in breast cancer

Author

Hai-Tao, Peng, Jian, Chen, Tian-Yu, Liu, Yan-Qing, Wu, Xiao-Hong, Lin, Yuan-Hui, Lai, and Ying-Feng, Huang

Subjects
Original Article
Abstract

Glyoxalase 1 (Glo1) is an enzyme that plays a role to metabolize and inactivate methylglyoxal. Previous studies also have confirmed that Glo1 is closely related with tumorigenesis, metastasis, and drug-resistant, but its prognostic value in breast cancer has never been explored. In this study, we investigated the expression of Glo1 in breast cancer cell lines and tissues using real-time PCR, western blot and immunohistochemical analysis. We found Glo1 was frequently up-regulated in human breast cancer cells and tissues, and high expression of Glo1 was associated with positive lymph node, lymphovascular invasion, and TNM stage (all P

Published
2017

8. [Full sequence analysis for a null allele of MICA gene (MICA*063N)]

Author

Ying-feng, Huang, Yin, Tan, Shao-yu, Yang, Hong-tu, Luo, and Tai-chen, Zou

Subjects
Base Sequence, Histocompatibility Antigens Class I, Molecular Sequence Data, Codon, Terminator, Humans, Female, Exons, Middle Aged, Sequence Analysis, Alleles
Abstract

To analyze the full nucleotide sequence of a null allele of major histocompatibility complex class I chain-related gene (MICA).A sequence-based typing method was used to determine the nucleotide sequence of the MICA gene. Potential alleles were identified with a computer program.The identified allele has possessed a sequence similar to that of MICA*027 except for a C→T substitution at position 184 in codon 62 (CAG→TAG) of exon 2. As a stop codon, this may result in a truncated protein.A null allele of MICA gene has been identified. The sequence has been submitted to the Genbank nucleotide sequence database (submission No. HWS10011131), which was officially named as MICA*063N by the WHO Nomenclature Committee in October 2010.

Published
2012

9. [Preparation of doxorubicin encapsulated in amphiphilic polysaccharide nanoparticles and anti-hepatocarcinoma effect thereof]

Author

Ying-Feng, Huang, Wei-Li, Gu, Zhi-Hua, Li, Ru-Fu, Chen, Jia-Jia, Zhou, Quan-Bo, Zhou, and Ning, Guo

Subjects
Drug Carriers, Mice, Mice, Inbred BALB C, Carcinoma, Hepatocellular, Doxorubicin, Cell Line, Tumor, Liver Neoplasms, Animals, Humans, Mice, Nude, Nanoparticles
Abstract

To investigate the sustained release rule of doxorubicin/polylactide-grafted dextran copolymer (DOX/DEX-PLA) nanoparticles and the effect thereof in killing hepatocarcinoma cells.DOX/DEX-PLA nanoparticles were prepared by method of emulsificationevaporation of organic solvent. Its morphology was observed by transmission electron microscopy and the encapsulating efficiency of DOX was determined by ultraviolet spectrophotometry. DOX/DEX-PLA was put in a dialysis bag to observe the releasing characteristics of DOX from DOX/DEX-PLA nanoparticles in vitro. Human liver carcinoma cells of the line HepG2 were cultured with DOX/DEX-PLA of different concentrations or the original drug of DOX as control group for 24, 36, or 48 h. MTT method was used to observe the cancer inhibition rate. BALB/c nude mice underwent subcutaneous injection of HepG2 cells at the right scapula and then randomly divided into 4 groups to undergo intravenous injection of DOX/DEX-PLA (excremental group), original drug of DOX (naked drug group), DEX-PLA (nanovector group), or normal saline (blank control group) once every 5 days for 3 times. Twenty-one days later the mice were killed with the tumors taken out to measure the weight to analyze the inhibitory effect against hepatocarcinoma cells.The DOX/DEX-PLA nanoparticles were of round or elliptical shape with the diameter of about 83 nm, and the DOX entrapment efficiency was about 67.1%. The releasing test in vitro manifested a sustained release of over 50% of DOX encapsulated in DOX/DEX-PLA nanoparticles for about 7 days. Both the DOX/DEX-PLA nanoparticles and naked drug DOX inhibited the growth of HepG2 cells with a similar inhibitory rate (51.3% vs 50.7%, P0.05), meanwhile the DEX-PLA nanovector failed to inhibit the HepG2 cells. In-vivo experiment showed an inhibitory rate of DOX/DEX-PLA nanoparticles on hepatocellular carcinoma xenografts of 68.56%, significantly higher than that of the naked drug DOX (48.17%).DOX/DEX-PLA nanoparticles can effectively inhibit the growth of hepatocarcinoma cells.

Published
2009

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