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Spatial data is becoming increasingly important in forestry resource management and decision making. However, huge amount of accumulated spatial data were not fully utilized due to the difficulty of sharing and integration. A forest spatial data sharing and integration system was developed based on GeoServer. A distributed architecture was adopted to address the data storage in different organizations. WMS and WFS were the main service for data sharing and integration. Applications demonstrate that the system can easily share and integrate various spatial data.

A GIS based decision support system for eucalypt cultivation and management was developed. The system can help farmers make decision in planting suitability, eucalypt variety selection, planting scheme making etc. The system can also make an economic evaluation for the selected variety so that users can make decision to plant or not. The system supports two groups of users which are eucalypt farmers who cultivate eucalypt in a small scale and forest farms who cultivate eucalypt in a relatively large scale.

We have previously defined the anatomy of the neurovascular bundle in the normal and hypospadiac penis. Historical experience suggests that mobilization of the neurovascular bundle is anatomically possible. We attempt to prove whether mobilization of the neurovascular bundle is safe and theoretically sound. Specific questions that will be addressed are does the neurovascular bundle send perforating branches into the corporal bodies; how far lateral does the dissection need to be before nerves are injured and exactly how deep into Buck's fascia must one go.A total of 35 normal human fetal penile specimens, gestational age 8 to 35 weeks, and 3 hypospadiac specimens, 33 to 41 weeks of gestation, were serially sectioned and stained with Mason's trichrome and the neuronal markers PGP 9.5 or S100. Computer reconstruction using commercial software and National Institutes of Health imaging allowed 3-dimensional analysis of the nerves, corporal bodies and glans.Perforating nerves into the erectile bodies were not documented along the dorsal or lateral aspect of the tunica in any of the specimens studied. Only in the area of the crural bodies on the ventral lateral surface were nerves noted to pierce into erectile tissue. The neural network was extensive from the 11 and 1 to the 5 and 7 o'clock positions corresponding to the erectile tissue and urethral spongiosum junction. At this junction minor nerve branches were noted to perforate into the urethral spongiosum. A microscopic plane exists between the neurovascular bundle and tunica of the corporal bodies measuring 20 to 30 micro. in specimens greater than 30 weeks in gestation.Perforating branches from the dorsal lateral neurovascular bundle do not exist based on serial step sectioning and microscopic examination of male genital specimens. Surgically it is possible to elevate the neurovascular bundle but the dissection needs to remain directly on top of the tunica albuginea to prevent neuronal injury. Small perforating branches into the urethral spongiosum may be injured with unknown significance. We continue to advocate plication in the nerve-free zone at the 12 o'clock position for correction of penile curvature.

Endogenous or exogenous testosterone exposure to the fetus during gestation may result in masculinization of the external genitalia. Surgical correction requires a clear understanding of normal female anatomy. We report observations from our anatomical dissections on which we base our approach to reduction clitoroplasty.A total of 14 normal human fetal clitoral specimens at 8 to 24 weeks of gestation were serially sectioned after formalin fixation. Every tenth section was stained with Masson's trichrome, smooth muscle alpha-actin and the neuronal markers PGP 9.5 or S-100. Computer reconstruction using imaging software permitted 3-dimensional analysis of the nerves, corporeal bodies and glans clitoris. These specimens were compared with 2 obtained postnatally at feminizing genitoplasty.The normal fetal clitoris consists of 2 corporeal bodies with a midline septum. The ultrastructure of the female corporeal bodies is similar to that of the male counterpart. The glans clitoris forms a cap on top of the distal end of the narrowed corporeal bodies. There is a midline septum starting on the ventral aspect and extending approximately halfway into the glans. Large bundles of nerves course along the corporeal bodies with the highest density on the dorsal aspect or top. No nerves were noted at the 12 o'clock position, although nerves extend completely around the tunica in a fashion similar to that of the fetal penis. Glans innervation is provided by multiple perforating branches entering at the dorsal junction of the corporeal body and glans. The lowest density of nerves in the glans is on the ventral aspect in juxtaposition to the glans septum. In surgical specimens obtained from patients with congenital adrenal hyperplasia nerves were adjacent to the excised tunica of the corporeal bodies, especially on the lateral aspect.A clear understanding of the anatomy of the human clitoris is important for surgical reconstruction. As in the human penis, nerves in the clitoris form an extensive network around the tunica of the corporeal body with a nerve-free zone at the midline 12 o'clock position. Care should be taken to preserve all nerves. Reduction of the glans clitoris should not violate the extensive innervation that predominates on the dorsal aspect of the glans. The normal clitoris has corporeal bodies that are smaller but analogous to those of the penis. One may consider their function when extensive resection is considered.

Hypospadias is the most common congenital anomaly affecting the penis. Successful repair depends on an accurate understanding of anatomy. We compared the anatomy of hypospadiac and normal fetal penises.A fetal penis at 33 weeks of gestation with distal shaft hypospadias was serially sectioned and compared to 10 normal human fetal specimens at 8 to 32 weeks of gestation. Immunohistochemical localization was performed with S-100 protein and protein gene product 9.5 to localize neurons. Blood vessels were localized by the presence of red corpuscles, and immunohistochemical staining with von Willebrand's factor and factor VIII. Three-dimensional computer reconstructions of the nerves, corporeal bodies, tunica and urethra of the hypospadiac and normal fetal penises were compared.Except at the region of the abnormal urethral spongiosum and glans, the hypospadiac and normal penises showed no difference in neuronal innervation, corpora cavernosa and tunica albuginea architecture and blood supply. The nerves started proximally as 2 well defined bundles under the pubic rami superior and slightly lateral to the urethra. As the 2 crural bodies converged into the corpora cavernosa, the nerves diverged, spreading around the cavernous bodies up to the junction with the urethral spongiosum without remaining at the 11 and 1 o'clock positions. Along the entire shaft of the penis there were no neuronal structures at the 12 o'clock position. The most striking difference was in vascularity. In the hypospadiac penis factor VIII immunostaining revealed huge endothelial lined vascular channels filled with red blood cells. In contrast, the normal penis had well defined small capillaries around the urethra that fanned out into the glans. Vascularity was also extensive under the urethral plate. Nerve distribution in the abnormal glans was also less extensive than in the normal penis.Increased knowledge of normal and hypospadiac penile anatomy with respect to the nerves, corporeal bodies, glans and vascularity is useful for the strategic design of penile reconstructive procedures.

The urinary bladder has two major functions: 1) storage and 2) socially acceptable efficient emptying.1 To achieve this end the bladder has evolved as a highly compliant muscular sac with a specialized epithelial lining able to accommodate extensive variations in internal dimensions while maintaining an impervious luminal surface. Mesenchymal-epithelial interactions play an important role in the differentiation of both the urothelium and the smooth muscle of the bladder.2 These interactions are also important in maintaining homeostasis and in responding to injury and insults in adulthood.3 There is evidence to support the idea that stromal-epithelial interactions also play important roles in both carcinoma of the urinary bladder4,5 and in fibrotic responses to urinary obstruction due to a number of causes.6 Such fibrotic responses lead to the formation of a non-compliant bladder, that is, an organ which is unable to expand sufficiently to maintain the low pressure internal environment required to both store urine and protect the kidneys from damage due to urinary reflux.

During embryogenesis we have previously shown that urothelium is essential for normal bladder growth and development. Urothelial growth may be mediated by peptides of the epidermal growth factor family, since the epidermal growth factor receptor is expressed in bladder urothelium and epidermal growth factor has been shown to induce deoxyribonucleic acid synthesis and migration of urothelial cells in vitro. Bladders from transgenic mice in which the epidermal growth factor receptor gene has been knocked out were used to examine the possible role of epidermal growth factor in bladder growth and development, detrusor neoformation and bladder regeneration.Whole bladders from transgenic knockout mice 0 to 10 days old were surgically implanted into the "subdetrusor" space of adult athymic nude rat hosts. After 10 days the dome of the host rat bladder was resected with the distal half of the transplanted knockout mouse bladder. Augmentation cystoplasty was then performed on the host rat bladder using acellular tissue matrix with a portion of the acellular matrix sutured directly to the transplanted knockout mouse bladder. The animals were sacrificed 2 or 3 weeks postoperatively. To test the ability of knockout bladder tissue to regenerate into the transplanted matrix species specific Hoechst dye was used to determine whether the cells within the acellular matrix were of host (rat) or transplant (knockout mouse) origin. Immunocytochemical analysis was used to assess muscle neoformation. Controls consisted of wild-type mouse bladders from the same litter. Since epidermal growth factor receptor knockout mice usually die in the neonatal period, the role of the epidermal growth factor receptor signaling pathway in long-term muscle development was evaluated by transplanting knockout and wild-type control bladders under the renal capsule of athymic nude mouse hosts. These mice were sacrificed 30 days later and muscle development was assessed using immunocytochemical analysis.Histologically the transplanted acellular tissue matrix in the experimental and control animals appeared the same, containing well differentiated urothelial and smooth muscle cells that had migrated into the transplanted matrix. Staining with species specific Hoechst dye revealed that urothelial and smooth muscle cells transplanted from the knockout and wild-type mouse bladders invaded and regenerated in the transplanted matrix. There was no apparent difference in the amount of knockout or control mouse tissue in the transplanted matrix. Also, the long-term renal capsule transplants revealed no difference in the amount of smooth muscle in the epidermal growth factor receptor knockout and wild-type bladders.Signaling through the epidermal growth factor receptor pathway is not necessary for normal bladder development or bladder regeneration after injury.