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1. Analysis of the Class 1 Integrons, Carbapenemase Genes and Biofilm Formation Genes Occurrence in Acinetobacter baumannii Clinical Isolates

Author

Xiu Yu, Dai Yueru, Yin Shasha, and Wei Quhao

Subjects
integron, virulence gene, carbapenem, antimicrobial resistance, acinetobacter baumannii, Genetics, QH426-470, Microbiology, QR1-502
Abstract

Acinetobacter baumannii is a non-fermentative Gram-negative bacterium that can cause nosocomial infections in critically ill patients. Carbapenem-resistant A. baumannii (CRAB) has spread rapidly in clinical settings and has become a key concern. The main objective of this study was to identify the distribution of integrons and biofilm-formation-related virulence genes in CRAB isolates. A total of 269 A. baumannii isolates (219 isolates of CRAB and 50 isolates of carbapenem-sensitive A. baumannii (CSAB)) were collected. Carbapenemase genes (blaKPC, blaVIM, blaIMP, blaNDM, and blaOXA-23-like) and biofilm-formation-related virulence genes (abal, bfms, bap, and cusE) were screened with PCR. Class 1 integron was screened with PCR, and common promoters and gene cassette arrays were determined with restriction pattern analysis combined with primer walking sequencing. Whole-genome sequencing was conducted, and data were analyzed for a blaOXA-23-like-negative isolate. All 219 CRAB isolates were negative for blaKPC, blaVIM, blaIMP, and blaNDM, while blaOXA-23-like was detected in 218 isolates. The detection rates for abal, bfms, bap, and cusE in 219 CRAB were 93.15%, 63.93%, 88.13%, and 77.63%, respectively. Class 1 integron was detected in 75 CRAB (34.25%) and in 3 CSAB. The single gene cassette array aacA4-catB8-aadA1 with relatively strong PcH2 promoter was detected in class 1 integrons. The blaOXA-23-like-negative CRAB isolate was revealed to be a new sequence type (Oxford 3272, Pasteur 2520) carrying blaOXA-72, blaOXA-259, and blaADC-26. In conclusion, blaOXA-23-like was the main reason for CRAB’s resistance to carbapenems. A new (Oxford 3272, Pasteur 2520) CRAB sequence type carrying the blaOXA-72, blaOXA-259, and blaADC-26 was reported.

Published
2024
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17. Enabling High-Degree-of-Freedom Thermal Engineering Calculations via Lightweight Machine Learning.

Author

Tian, Yajing, Wang, Yuyang, Yin, Shasha, Lu, Jia, and Hu, Yu

Subjects
STEAM generators, MACHINE learning, THERMAL engineering, SENSITIVITY analysis, COOLANTS
Abstract

U-tube steam generators (UTSGs) are crucial in nuclear power plants, serving as the interface between the primary and secondary coolant loops. UTSGs ensure efficient heat exchange, operational stability, and safety, directly impacting the plant's efficiency and reliability. Existing UTSG models have fixed structures, which can only be used when certain parameters are given as model input. Such constraints hinder their ability to accommodate the diverse operating conditions, where input and output parameters can vary significantly. To address this challenge, we propose a machine learning-based method for developing a high-degree-of-freedom UTSG thermal model. The most notable feature of this approach is its capacity to flexibly interchange input and output parameters. By adopting comprehensive parameter sensitivity analysis, the most efficient method for training dataset generation is determined. Leveraging a lightweight machine learning method, the prediction accuracy for all UTSG parameters is improved to within 2.1%. The flexibility of the proposed machine learning approach ensures that the UTSG model can accommodate any type of parameter input without extensive reconfiguration of the model structure, thereby enhancing its applicability and robustness in real-world applications. [ABSTRACT FROM AUTHOR]

Published
2024
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27. Analysis of the Class 1 Integrons, Carbapenemase Genes and Biofilm Formation Genes Occurrence in Acinetobacter baumanniiClinical Isolates

Author

Xiu, Yu, Dai, Yueru, Yin, Shasha, and Wei, Quhao

Abstract

Acinetobacter baumanniiis a non-fermentative Gram-negative bacterium that can cause nosocomial infections in critically ill patients. Carbapenem-resistant A. baumannii(CRAB) has spread rapidly in clinical settings and has become a key concern. The main objective of this study was to identify the distribution of integrons and biofilm-formation-related virulence genes in CRAB isolates. A total of 269 A. baumanniiisolates (219 isolates of CRAB and 50 isolates of carbapenem-sensitive A. baumannii(CSAB)) were collected. Carbapenemase genes (blaKPC, blaVIM, blaIMP, blaNDM, and blaOXA-23-like) and biofilm-formation-related virulence genes (abal, bfms, bap, and cusE) were screened with PCR. Class 1 integron was screened with PCR, and common promoters and gene cassette arrays were determined with restriction pattern analysis combined with primer walking sequencing. Whole-genome sequencing was conducted, and data were analyzed for a blaOXA-23-like-negative isolate. All 219 CRAB isolates were negative for blaKPC, blaVIM, blaIMP, and blaNDM, while blaOXA-23-likewas detected in 218 isolates. The detection rates for abal, bfms, bap, and cusEin 219 CRAB were 93.15%, 63.93%, 88.13%, and 77.63%, respectively. Class 1 integron was detected in 75 CRAB (34.25%) and in 3 CSAB. The single gene cassette array aacA4-catB8-aadA1with relatively strong PcH2 promoter was detected in class 1 integrons. The blaOXA-23-like-negative CRAB isolate was revealed to be a new sequence type (Oxford 3272, Pasteur 2520) carrying blaOXA-72, blaOXA-259, and blaADC-26. In conclusion, blaOXA-23-likewas the main reason for CRAB’s resistance to carbapenems. A new (Oxford 3272, Pasteur 2520) CRAB sequence type carrying the blaOXA-72, blaOXA-259, and blaADC-26was reported.

Published
2024
Full Text
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