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9. Development and Evaluation of the Biological Adjuvant Bivalent Vaccine for Preventing Newcastle Disease and Infectious Bursal Disease

Author

Guo, Xiaochen, Sun, Wenying, Hu, Changhui, Wang, Xiangxiang, Li, Shuang, Qin, Ruihan, Wang, Yixuan, Liu, Jinmiao, Xue, Zhiqiang, Li, Huijuan, Zhang, Tingting, Wang, Wei, Guo, Yunpeng, Yin, Jiechao, Jiang, Ming, Zhao, Han, Wei, Lan, Zhang, Jingmin, and Ren, Guiping

Abstract

Newcastle disease (ND) and infectious bursal disease (IBD) are both one of the most economically important infectious diseases, which cause high infection rate and mortality of chickens and restrict the development of the poultry industry. Currently, vaccination is the most effective method to prevent ND and IBD. In this study, the biological adjuvant bivalent vaccine (rClone30-VP2L-chGM-CSF) was developed for preventing ND and IBD. Furthermore, the immune and protective effects of rClone30-VP2L-chGM-CSF were evaluated in specific-pathogen-free chickens. The experimental results indicated that chickens immunized with rClone30-VP2L-chGM-CSF vaccine significantly improved anti-IBDV and anti-NDV antibody titer; the level of CD4+ T, CD8+ T, B+, MHC-II+, and monocyte/macrophagocyte+ cells; the concentrations of IL-1β, IL-4, IL-17, IFN-α, IFN-β, and IFN-γ; and the splenocyte proliferation rate. Anti-NDV antibody titer reached the theoretical protection value of 4log2 at 7 days after immunization in chickens of rClone30-VP2L-chGM-CSF group; however, which at 14 days after immunization in chickens of rClone30-VP2L and rClone30. These results showed that chickens immunized with rClone30-VP2L-chGM-CSF stimulated stronger immune response than those with the rClone30-VP2L and rClone30. Chickens were challenged with virulent IBDV BC6/85, which were protected in the rClone30-VP2L-chGM-CSF group. Furthermore, IBDV RNA was not measured, and there appeared to be little apoptosis in the bursa of Fabricius in the rClone30-VP2L-chGM-CSF group. Therefore, rClone30-VP2L-chGM-CSF is a promising vaccine candidate against infectious bursal disease virus (IBDV) and newcastle disease virus (NDV), and it provides an idea for developing other poultry vaccines.

Published
2023
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17. A Descriptive Analysis of Computer-Assisted Teaching and Learning in Molecular Biological Education

Author

Li, Guangxing, Yin, Jiechao, and Ren, Yudong

Abstract

The role and importance of computer-assisted teaching and learning in molecular biological-related education and research has been emphasized and pinpointed. In this study, some benefit viewpoints and discussion are provided for applying the computer-assisted teaching and learning more efficiently in the process of knowledge acquisition and information exchange. Meanwhile, several cultural and economic issues related to the role of computers in teaching and learning are addressed.

Published
2006
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18. Development and evaluation of a recombinant VP2 neutralizing epitope antigen vaccine candidate for infectious bursal disease virus

Author

Guo, Xiaochen, primary, Sun, Wenying, additional, Wei, Lan, additional, Wang, Xiangxiang, additional, Zou, Yimeng, additional, Zhang, Yingying, additional, Li, Shuai, additional, Wang, Nan, additional, Jiang, Ming, additional, Zhao, Han, additional, Qu, Enbo, additional, Pang, Yuqing, additional, Yin, Jiechao, additional, and Ren, Guiping, additional

Published
2021
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21. The recombinant Newcastle disease virus Anhinga strain expressing human TRAIL exhibit antitumor effects on a glioma nude mice model

Author

He, Jinjiao, primary, An, Ying, additional, Qi, Jianying, additional, Cui, Lin, additional, Yang, Kai, additional, Liu, Mingyao, additional, Qu, Bo, additional, Yan, Shijun, additional, Yin, Jiechao, additional, Jing, Xiaohui, additional, Dong, Hui, additional, Yu, Qingzhong, additional, Li, Deshan, additional, and Wu, Yunzhou, additional

Published
2020
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23. Optimization of oncolytic effect of Newcastle disease virus Clone30 by selecting sensitive tumor host and constructing more oncolytic viruses

Author

Liu, Tianyan, primary, Zhang, Yu, additional, Cao, Yukai, additional, Jiang, Shan, additional, Sun, Rui, additional, Yin, Jiechao, additional, Gao, Zhenqiu, additional, Ren, Guiping, additional, Wang, Zhenzhong, additional, Yu, Qingzhong, additional, Sui, Guangchao, additional, Sun, Xu, additional, Sun, Wenying, additional, Xiao, Wei, additional, and Li, Deshan, additional

Published
2020
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24. The recombinant Newcastle disease virus Anhinga strain expressing human TRAIL exhibit antitumor effects on a glioma nude mice model.

Author

He, Jinjiao, An, Ying, Qi, Jianying, Cui, Lin, Yang, Kai, Liu, Mingyao, Qu, Bo, Yan, Shijun, Yin, Jiechao, Jing, Xiaohui, Dong, Hui, Yu, Qingzhong, Li, Deshan, and Wu, Yunzhou

Subjects
NEWCASTLE disease virus, GLIOMAS, IMMUNE checkpoint inhibitors, RECOMBINANT viruses, ANIMAL disease models
Abstract

Oncolytic virus therapy is perhaps the next major breakthrough in cancer treatment following the success in immunotherapy using immune checkpoint inhibitors. However, the potential oncolytic ability of the recombinant newcastle disease virus (NDV) Anhinga strain carried with tumor necrosis factor‐related apoptosis inducing ligand (TRAIL) has not been fully explored at present. In the present study, the recombinant NDV/Anh‐TRAIL that secretes soluble TRAIL was constructed and the experiment results suggested NDV/Anh‐TRAIL as a promising candidate for glioma therapy. Growth kinetic and TRAIL secreted quantity of recombinant NDV/Anh‐TRAIL virus were measured. Cytotoxic and cell apoptosis were analyzed for its anti‐glioma therapy in vitro. Nude mice were used for the in vivo evaluation. Both tumor volume and mice behavior after injection were observed. The recombinant virus replicated with the same kinetics as the parental virus and the highest expression of TRAIL (77.8 ng/L) was found at 48 hours. The 3‐(4,5‐dimethylthiazol‐2‐yl)‐2,5‐diphenyltetrazolium bromide, a tetrazole and flow cytometry data revealed that the recombinant NDV/Anh‐TRAIL (56.1 ± 8.2%) virus could induce a more severe apoptosis rate, when compared with the NDV wild type (37.2 ± 7.0%) and mock (7.0 ± 1.8%) groups (P <.01), in U251 cells. Furthermore, in the present animal study, the average tumor volume was smaller in the NDV/Anh‐TRAIL group (97.21 mm3), when compared with the NDV wild type (205.03 mm3, P <.05) and PBS (310.30 mm3, P <.01) groups. Highlights: NDV/Anh‐TRAIL exerts an obvious result in U251 glioma therapeutic effect in vitro and in vivo. Anhinga strain could be an effectual vector for tumor therapy. NDV/Anh‐TRAIL could become a potent candidate for clinical treatment especially for glioma. [ABSTRACT FROM AUTHOR]

Published
2021
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25. Optimization of oncolytic effect of Newcastle disease virus Clone30 by selecting sensitive tumor host and constructing more oncolytic viruses

Author

Liu, Tianyan, Zhang, Yu, Cao, Yukai, Jiang, Shan, Sun, Rui, Yin, Jiechao, Gao, Zhenqiu, Ren, Guiping, Wang, Zhenzhong, Yu, Qingzhong, Sui, Guangchao, Sun, Xu, Sun, Wenying, Xiao, Wei, and Li, Deshan

Abstract

The direct oncolytic effect of Newcastle disease virus (NDV) depends on the following two aspects: the susceptibility of cancer cells to virus infection and the ability of virus itself to lyse cancer cells. First, we investigate the susceptibility of cancer cells to NDV infection, HepG2, MDA-MB-231, and SH-SY5Y cells were susceptible, A549, MCF7, and LoVo cells were less susceptible. To investigate the molecular mechanism responsible for cancer cell susceptibility, transcriptome sequencing was carried out. We found that the levels of alpha-sialic acid acyltransferase were upregulated in MDA-MB-231 cells compared with MCF7 cells, and the interferon was downregulated. Second, to optimize the oncolytic capacity of the wild-type rClone30, a series of chimeric viruses rClone30-Anh(HN), rClone30-Anh(F), and rClone30-Anh(HN-F) were constructed by exchanging the HN gene, F gene or both of non-lytic rClone30 strain with lytic strain Anhinga. rClone30-Anh(F) and rClone30-Anh(HN-F) enhanced the oncolytic effect of the rClone30, and this enhancement is more obvious in the susceptible cells. The oncolytic mechanism of rClone30-Anh(F) was analyzed by transcriptome analyses, in comparison with rClone30, rClone30-Anh(F) upregulated the expression of ATG5, Beclin 1, and MAP1LC3B, thus activating autophagy and promoting the production of syncytia. In conclusion, our study provides a strategy to enhance the oncolytic effect of rClone30.

Published
2021
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26. Neutralizing scFv Antibodies against Infectious Bursal Disease Virus Isolated From a Nlpa-Based Bacterial Display Library

Author

Tianhe Li, Ning Li, Bing Zhou, Tianyuan Zhang, Tingqiao Yu, Mingzhe Sun, Li Deshan, Jing-Zhuang Zhao, Liang-jun Ding, Yin Jiechao, Lei Ma, Liming Xu, Tingting Li, and Xiaochen Guo

Subjects
Bacterial display, General Energy, animal structures, medicine, ScFv Antibodies, Biology, medicine.disease, Virology, Virus, Infectious bursal disease
Abstract

GInfectious bursal disease (IBD) considered as one of the major viral diseases threatening the poultry industry worldwide. The causative agent of the IBD is Infectious bursal disease virus (IBDV) which replicates in developing B lymphocytes in the bursa of Fabricius leading to its destruction and bursal inflammation. In this study, we investigated a technology to produce therapeutic recombinant antibodies against IBDV in bacteria by constructing a bacterial displayed recombinant scFv library from immunized chickens, followed by screening the scFv library by fluorescence activated cell sorting (FACS) with FITC-labeled VP2. Twelve VP2-binding scFv clones with unique sequences were obtained, with overall amino acid homology of 81.53%. The complementarity determining region (CDR) 3 in the heavy chain displayed the lowest homology, while the amino acid sequences in framework regions and CDR2 of both chains and CDR1 of the heavy chain are relatively conserved. Twelve VP2-binding scFv clones were expressed in E.coli and purified through denaturation and denaturation of inclusion bodies. Our ELISA results showed that all scFvs exhibited binding ability and specificity to VP2 and various IBDV strains. In addition, two scFvs showed significant neutralizing activity to IBDV (B-87 strain) as these scFvs inhibited cytopathic effect of chicken embryo fibroblast (DF1) caused by IBDV. In conclusion, our study provides a lead candidate for further development of therapeutic antibodies for IBDV infection.

Published
2017
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30. Recombinant Newcastle disease virus expressing P53 demonstrates promising antitumor efficiency in hepatoma model

Author

An, Ying, primary, Liu, Tianyan, additional, He, Jinjiao, additional, Wu, Hongsong, additional, Chen, Rui, additional, Liu, Yunye, additional, Wu, Yunzhou, additional, Bai, Yin, additional, Guo, Xiaochen, additional, Zheng, Qi, additional, Liu, Chang, additional, Yin, Jiechao, additional, Li, Deshan, additional, and Ren, Guiping, additional

Published
2016
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